CN113475322A - Cutting seedling method for improving propagation coefficient of daylily by utilizing cytokinin - Google Patents
Cutting seedling method for improving propagation coefficient of daylily by utilizing cytokinin Download PDFInfo
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- CN113475322A CN113475322A CN202110581485.8A CN202110581485A CN113475322A CN 113475322 A CN113475322 A CN 113475322A CN 202110581485 A CN202110581485 A CN 202110581485A CN 113475322 A CN113475322 A CN 113475322A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention belongs to the technical field of short-stem seedling culture of day lily, and relates to a cutting seedling culture method for improving the propagation coefficient of the day lily by utilizing cytokinin, which breaks the apical dominance, utilizes a matrix to culture seedlings, sprays 6-BA solution with a certain concentration range on the short-stem of the day lily to promote the germination of main buds and hidden buds on the short-stem, ensures that the propagation coefficient reaches 2.12-2.30, and can grow seedlings and transplant after 35 days of seedling culture by utilizing the method; the method has the advantages of easy operation steps and low cost, is favorable for improving the improved variety expanding speed of the day lily, reducing diseases caused by cutting wound surfaces and keeping the stability of the genetic nature of vegetative propagation.
Description
Technical Field
The invention belongs to the technical field of short-stem seedling culture of day lily, and relates to a cutting seedling culture method for improving the propagation coefficient of day lily by utilizing cytokinin.
Background
Daylily (Hemerocallis sp.), also known as golden needle, is a perennial herb originating in temperate regions of asia and europe. The daylily has ornamental and edible values and is cultivated in the south and the north of China. The unique soil and climate conditions are very suitable for the growth of the day lily, and become one of the main production areas of the day lily in China.
The sexual propagation technology adopted by the great homology day lily seedlings is a seed propagation mode, but the seed germination rate is low, the offspring differentiation coefficient is large, the growth period of the day lily is longer, the childhood period is long, and the rapid propagation of the day lily is not facilitated. At present, the plant division propagation mode is commonly used for the vegetative propagation of the day lily, but the propagation rate of the method is low, the stem rot disease is easy to spread, and the method is not beneficial to the rapid propagation of fine varieties; with the development of science and technology, tissue culture is another daylily asexual propagation technology, but the method has high operation difficulty and high cost, and is not beneficial to wide popularization and use; at the present stage, the scape and the shortened stem of the flower can be used as materials for cuttage seedling raising of the day lily, but the scape is limited by time for obtaining materials, the rate of emergence is lower, and the success rate is not high; the shortened stem cuttage is mainly based on the propagation of buds on the roots of the homoeomorpha hemsleyana, but the technical requirement is high due to the fact that positions are difficult to distinguish by naked eyes before hidden buds germinate, and due to the fact that bud blocks/pieces are large in wound surface and small in nutrient, roots are easy to be injured, diseases are infected, and the seedling quality is affected.
Therefore, the development of a low-cost and simple-operation-management method for expanding improved variety of daylily is urgently needed, and the method is helpful for the continuous development and expansion of daylily industry.
The technical scheme of the prior art is as follows:
the cutting propagation method of the shortened stem is based on the position of a bud body (main bud, side bud or hidden bud) on a day lily bud slice, and can be divided into bud block propagation, slice propagation and bud division propagation.
The above-mentioned first prior art has the following disadvantages:
the hidden buds are difficult to distinguish before germination, so that the technical requirement is high, and the bud blocks/pieces are large in wound surface and small in nutrient body due to segmentation, so that roots are easy to hurt and diseases are easy to infect, and the seedling quality is influenced.
Disclosure of Invention
The invention provides a cutting seedling method for improving the propagation coefficient of daylily seedlings by spraying cytokinin, which is simple to operate, low in cost, short in time, high in seedling survival rate and propagation coefficient, and the specific technical scheme is as follows:
a cutting seedling method for improving the propagation coefficient of day lily by using cytokinin comprises the following steps:
step 1: selecting short stems of the day lily with complete buds and no diseases for later use; fully mixing turf, vermiculite and perlite to serve as a matrix;
step 2: filling the substrate into a seedling pot;
and step 3: cuttage, specifically:
placing the short stems of the daylily into the center of a seedling pot, filling the mixed substrate around the short stems, and thoroughly watering the substrate with water;
and 4, step 4: hormone treatment, in particular:
treating the short stalk of the day lily by cytokinin;
and 5: cuttage management, specifically:
watering at intervals of 2-3 days during the cutting seedling raising period, and keeping the humidity of the matrix, wherein the watering amount is 50 mL/seedling raising pot each time;
step 6: expanding propagation again, specifically:
after 20 days of cutting seedling raising, taking out the cutting seedling, separating the main bud and the side bud along the vertical direction between the main bud and the side bud which germinate by using a cutter, and ensuring that the separated main bud and the side bud both have partial shortened stems and fleshy roots;
and 7: and (3) cutting again, specifically:
cutting the separated main bud and the side bud again, and repeating the step 3;
and 8: and (5) carrying out cuttage management again: the operation is the same as the step 5;
and step 9: transplanting: after the cutting seedlings grow new leaves and new roots, the cutting seedlings can be directly taken out from the seedling pot for transplanting.
On the basis of the technical scheme, the turf, the vermiculite and the perlite in the step 1 are fully mixed according to the volume ratio of 2:1: 1.
On the basis of the technical scheme, the filling height of the substrate filled into the seedling raising pot in the step 2 is as follows: the length of the short stalk of the day lily is subtracted by 7cm to ensure that the short stalk is just positioned after the cutting seedling is put in: the distance between the seedling pot and the inner bottom of the seedling pot is 7 cm.
On the basis of the technical scheme, when the short stems of the day lily are placed in the positive center of the seedling pot, the short stems of the cutting seedlings are ensured to be positioned: the distance between the seedling pot and the inner bottom of the seedling pot is 7 cm.
On the basis of the technical scheme, the specific steps of the step 4 are as follows:
and spraying 6-BA solution on the short stems of the daylily.
On the basis of the technical scheme, the spraying concentration of the 6-BA solution in the step 4 is 100-150 mg/L.
On the basis of the technical scheme, the spraying method of the 6-BA solution in the step 4 comprises the following steps: spraying the mixture once every two days for 5 times.
On the basis of the technical scheme, in the cuttage management period in the step 5, the temperature of the cuttage environment is above 10 ℃;
geothermal wire heating may be employed to reduce extreme low temperature damage.
On the basis of the technical scheme, the cutter in the step 6 is a sterilized cutter.
On the basis of the technical scheme, the cutter in the step 6 is soaked in the carbendazim solution diluted by 1000 times for half an hour.
The invention has the following beneficial technical effects:
according to the cuttage seedling raising method for breaking the apical dominance by using cytokinin and improving the daylily seedling propagation coefficient, provided by the invention, the substrate is used for raising seedlings, the 6-BA solution with a certain concentration range is sprayed on the short stems of the daylily, the germination of main buds and hidden buds (lateral buds after the hidden buds germinate) on the short stems is promoted, the propagation coefficient reaches 2.12-2.30, and the seedlings can be grown for 35 days by using the method, so that the seedlings can be transplanted in a seedling forming manner; the method has the advantages of easy operation steps and low cost, is favorable for improving the improved variety expanding speed of the day lily, reducing diseases caused by cutting wound surfaces and keeping the stability of the genetic nature of vegetative propagation.
Detailed Description
The following describes the embodiments of the present invention in further detail with reference to examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
Example 1
A cutting seedling method for improving the propagation coefficient of day lily by using cytokinin comprises the following steps:
step 1: selecting short stems of the day lily with complete buds and no diseases for later use; fully mixing the grass carbon, the vermiculite and the perlite according to the volume ratio of 2:1:1 to obtain a matrix;
step 2: filling the substrate into a seedling pot; the filling height of the substrate filled into the seedling raising pot is as follows: subtracting the length of the short stems of the daylily from the length of 7cm to ensure that the short stems are positioned 7cm in the seedling pot after the cuttage seedlings are placed;
and step 3: cuttage, specifically:
placing the short stems of the daylily into the center of a seedling pot, filling the mixed substrate around the short stems, and thoroughly watering the substrate with water;
and 4, step 4: hormone treatment, in particular:
spraying a 6-BA solution with the concentration of 100mg/L on the short stems of the day lily, wherein the method comprises the following steps: spraying the mixture once every two days for 5 times;
and 5: cuttage management, specifically:
watering at intervals of 2-3 days during the cutting seedling raising period, and keeping the humidity of the matrix, wherein the watering amount is 50 mL/seedling raising pot each time; the temperature of the cuttage environment is above 10 ℃, and the geothermal heat line heating can be adopted to reduce the extreme low temperature damage.
Step 6: expanding propagation again, specifically:
after 20 days of cutting seedling raising, taking out the cutting seedling, soaking a cutter in a carbendazim solution diluted by 1000 times for half an hour, separating the main bud and the side bud in the vertical direction between the main bud and the side bud which germinate by using the sterilized cutter, and ensuring that the main bud and the side bud after separation have partial shortened stems and fleshy roots;
and 7: and (3) cutting again, specifically:
cutting the separated main bud and the side bud again, and repeating the step 3;
and 8: and (5) carrying out cuttage management again: the operation is the same as the step 5;
and step 9: transplanting: after the cutting seedlings grow new leaves and new roots, the cutting seedlings can be directly taken out from the seedling pot for transplanting.
The method test of this example was carried out using short, disease-free stems of daylily with consistent size, and the results of the experiment are shown in table 1, day lily cutting seedling with cytokinin in example 1, and the results of the experiment are compared with those of day lily cutting seedling with no 6-BA solution sprayed and with 100 mg/L6-BA solution sprayed, and the results are repeated 3 times for each treatment, one for each 5 plants
| No spraying of 6-BA solution | Spraying 100 mg/L6-BA solution | ||
| 10 days | Number of lateral buds | 0.067 | 1.3 |
| 20 days | Number of seedlings (propagation coefficient) | 1.067 | 2.3 |
| 35 days | Aerial biomass | 15.38g | 20.09g |
As can be seen from the comparison in Table 1, the method disclosed by the invention adopts 100 mg/L6-BA solution to spray the shortened stems of the daylily for 5 times and cut the germinated buds, the biomass of the overground parts of the cutting seedlings produced by the method is higher than that of the cutting seedlings which are not sprayed with hormone, and the propagation coefficient of the daylily is increased to 2.3.
Example 2
A cutting seedling method for improving the propagation coefficient of day lily by using cytokinin comprises the following steps:
step 1: selecting short stems of the day lily with complete buds and no diseases for later use; fully mixing the grass carbon, the vermiculite and the perlite according to the volume ratio of 2:1:1 to obtain a matrix;
step 2: filling the substrate into a seedling pot; the filling height of the substrate filled into the seedling raising pot is as follows: subtracting the length of the short stems of the daylily from the length of 7cm to ensure that the short stems are positioned 7cm in the seedling pot after the cuttage seedlings are placed;
and step 3: cuttage, specifically:
placing the short stems of the daylily into the center of a seedling pot, filling the mixed substrate around the short stems, and thoroughly watering the substrate with water;
and 4, step 4: hormone treatment, in particular:
spraying a 6-BA solution with the concentration of 150mg/L on the short stems of the day lily, wherein the method comprises the following steps: spraying the mixture once every two days for 5 times;
and 5: cuttage management, specifically:
watering at intervals of 2-3 days during the cutting seedling raising period, and keeping the humidity of the matrix, wherein the watering amount is 50 mL/seedling raising pot each time; the temperature of the cuttage environment is above 10 ℃, and the geothermal heat line heating can be adopted to reduce the extreme low temperature damage.
Step 6: expanding propagation again, specifically:
after 20 days of cutting seedling raising, taking out the cutting seedling, soaking a cutter in a carbendazim solution diluted by 1000 times for half an hour, separating the main bud and the side bud in the vertical direction between the main bud and the side bud which germinate by using the sterilized cutter, and ensuring that the main bud and the side bud after separation have partial shortened stems and fleshy roots;
and 7: and (3) cutting again, specifically:
cutting the separated main bud and the side bud again, and repeating the step 3;
and 8: and (5) carrying out cuttage management again: the operation is the same as the step 5;
and step 9: transplanting: after the cutting seedlings grow new leaves and new roots, the cutting seedlings can be directly taken out from the seedling pot for transplanting.
The method test of this example was performed using short, disease-free stems of daylily with consistent size, and the results of the test are shown in the following table, comparing the spraying of 6-BA solution and the spraying of 150 mg/L6-BA solution, each treatment was repeated 3 times for 5 plants,
TABLE 2 comparison of the results of the experiments for the cuttage and the seedling culture of day lily using cytokinin in example 2
| No spraying of 6-BA solution | Spraying 150 mg/L6-BA solution | ||
| 10 days | Number of lateral buds | 0.067 | 1.12 |
| 20 days | Number of seedlings (propagation coefficient) | 1.067 | 2.12 |
| 35 days | Aerial biomass | 15.38g | 14.58g |
As shown in the comparison in the table 2, the 150 mg/L6-BA solution is adopted to spray the shortened stems of the homoeopolydatin day lily for 5 times, and the germinated buds are cut and cut, the biomass of the overground parts of the cutting seedlings produced by the method is the same as that of the cutting seedlings which are not sprayed with the hormone, and the propagation coefficient of the day lily is improved to 2.12.
The invention is not limited to the foregoing embodiments. The invention extends to any novel feature or any novel combination of features presented in this specification, and to any novel method or process steps or any novel combination of steps presented.
Those not described in detail in this specification are within the knowledge of those skilled in the art.
Claims (10)
1. A cutting seedling method for improving the propagation coefficient of day lily by using cytokinin is characterized by comprising the following steps:
step 1: selecting short stems of the day lily with complete buds and no diseases for later use; mixing turf, vermiculite and perlite to serve as a matrix;
step 2: filling the substrate into a seedling pot;
and step 3: cuttage, specifically:
placing the short stems of the daylily into the center of a seedling pot, filling the mixed substrate around the short stems, and thoroughly watering the substrate with water;
and 4, step 4: hormone treatment, in particular:
treating the short stalk of the day lily by cytokinin;
and 5: cuttage management, specifically:
watering at intervals of 2-3 days during the cutting seedling raising period, and keeping the humidity of the matrix, wherein the watering amount is 50 mL/seedling raising pot each time;
step 6: expanding propagation again, specifically:
after 20 days of cutting seedling raising, taking out the cutting seedling, separating the main bud and the side bud along the vertical direction between the main bud and the side bud which germinate by using a cutter, and ensuring that the main bud and the side bud after separation are provided with shortened stems and fleshy roots;
and 7: and (3) cutting again, specifically:
cutting the separated main bud and the side bud again, and repeating the step 3;
and 8: and (5) carrying out cuttage management again: the operation is the same as the step 5;
and step 9: transplanting: and when the cutting seedlings grow new leaves and new roots, directly taking out the cutting seedlings from the seedling pot for transplanting.
2. The cutting seedling method for improving the propagation coefficient of daylily by using cytokinin as claimed in claim 1, characterized in that: and 1, mixing the turf, the vermiculite and the perlite according to a volume ratio of 2:1: 1.
3. The cutting seedling method for improving the propagation coefficient of daylily by using cytokinin as claimed in claim 1, characterized in that: and 2, filling the substrate into the seedling raising pot at a filling height of: the length of the short stem of daylily minus the length of the short stem is 7 cm.
4. The cutting seedling method for improving the propagation coefficient of daylily by using cytokinin as claimed in claim 3, characterized in that: when the short stems of the day lily are placed in the positive center of the seedling pot, the short stems of the cutting seedlings are ensured to be positioned: the distance between the seedling pot and the inner bottom of the seedling pot is 7 cm.
5. The cutting seedling method for improving the propagation coefficient of daylily by using cytokinin as claimed in claim 1, characterized in that: the specific steps of the step 4 are as follows:
and spraying 6-BA solution on the short stems of the daylily.
6. The cutting seedling method for improving the propagation coefficient of daylily by using cytokinin as claimed in claim 5, characterized in that: the spraying concentration of the 6-BA solution in the step 4 is 100-150 mg/L.
7. The cutting seedling method for improving the propagation coefficient of daylily by using cytokinin as claimed in claim 6, wherein: the spraying method of the 6-BA solution in the step 4 comprises the following steps: spraying the mixture once every two days for 5 times.
8. The cutting seedling method for improving the propagation coefficient of daylily by using cytokinin as claimed in claim 1, characterized in that: during the cuttage management period in the step 5, the temperature of the cuttage environment is above 10 ℃;
hot wire heating is used to reduce extreme low temperature damage.
9. The cutting seedling method for improving the propagation coefficient of daylily by using cytokinin as claimed in claim 1, characterized in that: and 6, the cutter is a sterilized cutter.
10. The cutting seedling method for improving the propagation coefficient of daylily by using cytokinin as claimed in claim 9, characterized in that: and 6, soaking the cutter in the carbendazim solution diluted by 1000 times for half an hour.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114190251A (en) * | 2021-12-03 | 2022-03-18 | 云南农业大学 | Induction method for promoting proliferation of babysbreath lateral buds |
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Application publication date: 20211008 |