CN113457211A

CN113457211A - A purify device fast for pregnant horse serum gonadotropin

Info

Publication number: CN113457211A
Application number: CN202110798541.3A
Authority: CN
Inventors: 董建宝; 朱伟; 高楠楠; 索佳佳; 杜燕; 樊书杉; 袁东芳
Original assignee: Shandong Vocational Animal Science and Veterinary College
Current assignee: Shandong Mingzhou Wuma Biotechnology Co Ltd
Priority date: 2021-07-15
Filing date: 2021-07-15
Publication date: 2021-10-01
Anticipated expiration: 2041-07-15
Also published as: CN113457211B

Abstract

The invention belongs to the technical field of pregnant mare serum gonadotropin purification, and discloses a rapid purification device for pregnant mare serum gonadotropin, which comprises a mixing bin, a cooling bin, a belt pulley transmission mechanism, a rotary cylinder and a funnel, wherein the rotary cylinder is in transmission connection with the belt pulley transmission mechanism, and the top of the mixing bin is covered with a top cover. According to the invention, alcohol is injected into the rotary cylinder and the inner cylinder, and the belt pulley transmission mechanism drives the rotary cylinder and the release cylinder to continuously rotate, so that under the condition that the inner cylinder is completely filled with the alcohol, a second through hole on the surface of the inner cylinder is overlapped with a first through hole on the surface of the outer cylinder by utilizing a centrifugal trend, so that the inner spaces of the inner cylinder and the cooling bin are communicated, the alcohol can be synchronously and uniformly released at each part, the speed of the released position is influenced by the rotating speed, the phenomenon that the local concentration is too high due to the fact that the alcohol is added at the same part is avoided, and the phenomenon that the pregnant mare serum gonadotropin loses activity is avoided.

Description

A purify device fast for pregnant horse serum gonadotropin

Technical Field

The invention belongs to the technical field of pregnant mare serum gonadotropin purification, and particularly relates to a rapid purification device for pregnant mare serum gonadotropin.

Background

Pregnant Mare Serum Gonadotropin (PMSG) is glycoprotein, can be extracted from pregnant mare plasma, can mainly aim at target cell receptors of ovaries and testicles in animals, can promote the development, maturity, ovulation and luteal generation of follicles, can promote the formation of sperms and the development of supporting cells when male animals act on the seminal tubules of the testicles, is widely applied to the treatment of infertility of livestock and the promotion of oestrus of the animals, and plays an indispensable role in the aspect of protecting endangered animals and promoting the reproduction of the animals.

The extraction and purification of pregnant mare serum gonadotropin are mainly to extract from mare serum during pregnant mare, other impure proteins in serum need to be removed in the purification, generally, metaphosphoric acid is selected to adsorb impure proteins industrially, and alcohol is used for precipitation, in the process, the using amount of alcohol is large, and the purity of alcohol is high, and reaches ninety-five percent, so that when the alcohol is mixed with pregnant mare serum, heat can be released, pregnant mare serum gonadotropin is a heat-sensitive glycoprotein, and the temperature is slightly high, and the activity can be lost, so the existing means for adding alcohol is to refrigerate the alcohol at minus fifteen degrees or so, then use, and need to slowly inject and continuously stir the serum, firstly, the hormone activity loss caused by the overhigh local alcohol concentration is avoided, and the heat production activity loss caused by the excessive alcohol added at one time is avoided, therefore, the speed of mixing and adding the sediment is very slow, the speed is slower because no professional equipment is arranged in a laboratory, the mode of dripping alcohol is used, and the stirring is continued, so the operation is not only complicated, but also the production efficiency and the working progress are greatly influenced in industrial production or laboratory preparation, therefore, the invention provides a novel purification device which is mainly used for accelerating the speed of adding and mixing the alcohol, maintaining the hormone activity and improving the working efficiency.

Disclosure of Invention

The invention aims to provide a device for quickly purifying pregnant mare serum gonadotropin, which solves the problems in the background technology.

In order to achieve the above purpose, the invention provides the following technical scheme: a quick purification device for pregnant mare serum gonadotropin comprises a mixing bin, a cooling bin, a belt pulley transmission mechanism, a rotating drum and a funnel, wherein the rotating drum is in transmission connection with the belt pulley transmission mechanism, the top of the mixing bin is also covered with a top cover, the funnel is fixedly installed at the top of the top cover, a bottom output pipe of the funnel is downwards inserted into the rotating drum but is not in contact with the rotating drum, the mixing bin is placed in the middle of an inner cavity of the cooling bin, the rotating drum downwards extends into the inner cavity of the mixing bin, the outer surface of the bottom of the rotating drum is fixedly communicated with seven release drums at equal intervals from top to bottom, the bottom of the rotating drum is sealed and does not flow, the seven release drums are all positioned in the inner cavity of the mixing bin, each release drum comprises an outer drum and a first through hole formed in the surface of the outer drum, an inner drum is sleeved in the inner cavity of the outer drum, a second through hole is formed in the surface of the inner drum, and the second through hole is staggered with the first through hole in an initial state, the inner cavity of the inner barrel is internally provided with a balance weight ball, the front end opening of the outer barrel is provided with a bottom plug, the inner cavity of the outer barrel is also internally provided with a spring, two ends of the spring are respectively and elastically supported on the inner barrel and the bottom plug, and when the spring is stressed by the inner barrel to reach the maximum compression amount, the second through hole is coincided and aligned with the first through hole and the inner cavity of the inner barrel is communicated with the external environment of the outer barrel through the first through hole and the second through hole.

Preferably, the inner wall laminating contact of piston and urceolus is installed to the tip opening part of inner tube, the surface of urceolus still is equipped with the stirring leaf, all seted up the fretwork groove on end plug and the piston, the fretwork groove of end plug is for the exterior space of intercommunication spring place space and urceolus, avoids having the unable discharge of here air that the sealing brought, still receives atmospheric pressure's negative effects when leading to inner tube compression spring.

Preferably, the stiffness coefficients of the springs in the seven release cylinders from top to bottom are gradually decreased, so that the liquid level is raised by the raw materials, the alcohol and other solution moderators in the mixing bin along with the injection of the alcohol, and the release cylinder located relatively below bears the water pressure of a deeper water level, so that the pressure from the outside borne by the inner cylinder located below is greater due to the combined action of the water pressure and the springs, and therefore, the elastic force of the springs on the inner cylinder located below needs to be reduced, so that the total pressure borne by the inner cylinder is negatively compensated.

Preferably, the inner cavity wall of the cooling bin is provided with a limiting ring, the top of the limiting ring is provided with a first blocking net, the inner ring of the first blocking net is sleeved on the outer surface of the mixing bin, an annular columnar cavity is formed between the inner cavity of the cooling bin and the outer wall of the mixing bin and is divided into an upper annular columnar cavity and a lower annular columnar cavity through the first blocking net, because the density of the first blocking net is less than that of water, when the water submerges the first blocking net, ice blocks can float along with the first blocking net, this can result in the water at the bottom of the lower annular cylindrical cavity being at a slightly higher temperature due to insufficient contact with the ice, the water pumped by the water pump for cooling is pumped from the bottom of the lower annular columnar cavity, so that the ice blocks are blocked in the lower annular columnar cavity by a first blocking net, and the water pumped by the water pump is cooled.

Preferably, an ice-water mixture is filled in the lower annular cylindrical cavity, an ice-water feeding column is fixedly installed on the outer wall of the cooling bin, the bottom of the ice-water feeding column is communicated with the lower annular cylindrical cavity of the cooling bin, workers can directly feed ice blocks into the opening at the top of the ice-water feeding column and inject water into the ice blocks, the ice blocks and the water can enter the lower annular cylindrical cavity, and new ice blocks can be added into the lower annular cylindrical cavity when the ice blocks and the water need to be added later.

Preferably, a water pump is arranged outside the cooling bin, the lower annular columnar cavity of the cooling bin is communicated with the water pump through a connecting pipe, a second blocking net is arranged inside the connecting pipe and is used for blocking ice blocks to avoid pipeline blockage caused by pumping the ice blocks, the water pump is communicated with a water separator arranged at the top of the mixing bin through a communicating pipe and pumps water in the lower annular columnar cavity into an inner cavity of the water separator, a circle of water outlets are formed in the bottom of the water separator at equal intervals, water flowing out from the water outlets can flow onto the surface of the cooling bin and then flow down along the surface of the cooling bin, and due to the fact that water flows are diffused on a curved surface and a flat plate, even if only the position where the water outlets are formed at the bottom of the water separator can leak water, the water flowing out from each water outlet flows down along the surface of the cooling bin, the coalescence is still formed due to the diffusion phenomenon, so that the outer surface of the cooling chamber is completely covered.

Preferably, the top edge of the mixing bin is designed to be a round angle, water in the inner cavity of the water separator flows down to the round angle of the outer edge of the mixing bin through the water outlet and flows down along the outer surface of the mixing bin, the bottom of the inner ring of the water separator is further provided with a snap ring which is clamped on the inner side of the top of the mixing bin, the round angle design can prevent water from splashing after falling onto the mixing bin, the flowing of the water is more natural and smooth, the situation that splashing water possibly falls into the inner cavity of the mixing bin to cause impure supernatant liquid to be finally prepared is avoided, the heat generated on the mixing bin can be directly taken away by the flowing water flow, and the heat exchange speed between the raw material and the outside in the mixing bin is accelerated.

Preferably, the surface of a rotatory section of thick bamboo still is equipped with the spiral leaf, and the spiral leaf can play the effect of an auxiliary mixing, and as shown in the figure, when a rotatory section of thick bamboo drove spiral leaf anticlockwise rotation, the spiral leaf can upwards stir the raw materials in the mixing bin inner chamber to accelerate the diffusion rate of inside alcohol in the raw materials.

Preferably, the number of the first through holes formed in the surface of the outer cylinder is at least five and at most eight, the opening directions of the first through holes are different, the number of the second through holes formed in the inner cylinder is equal to the number of the first through holes, namely, each first through hole needs one second through hole with the same opening direction to coincide with the first through hole, so that the inner cavity of the inner cylinder and the outer cylinder are communicated, the opening directions of the first through holes are different, and the purpose of diffusing the alcohol discharged from the inner cavity of the inner cylinder to all directions of the inner cavity of the mixing bin is realized.

Preferably, the top of top cap still is equipped with a feeding column and No. two feeding columns, the bottom opening of a feeding column and No. two feeding columns all faces the inner chamber of mixing the storehouse, can directly pour into raw materials and other reagent into the inner chamber of mixing the storehouse from a feeding column and No. two feeding columns.

The invention has the following beneficial effects:

1. according to the invention, alcohol is injected into the rotary cylinder and the inner cylinder, and the belt pulley transmission mechanism drives the rotary cylinder and the release cylinder to continuously rotate, so that under the condition that the inner cylinder is completely filled with the alcohol, a second through hole on the surface of the inner cylinder is overlapped with a first through hole on the surface of the outer cylinder by utilizing a centrifugal trend, so that the inner spaces of the inner cylinder and the cooling bin are communicated, the alcohol can be synchronously and uniformly released at each part, the speed of the released position is influenced by the rotating speed, the phenomenon that the local concentration is too high due to the fact that the alcohol is added at the same part is avoided, and the phenomenon that the pregnant mare serum gonadotropin loses activity is avoided.

2. According to the invention, in the synchronous rotation process of the rotary cylinder, the release cylinder and the spiral blade, the serum and the alcohol are mixed and stirred simultaneously, so that the diffusion and mixing speed of the alcohol is increased, the faster the rotation speed is, the stirring speed is in direct proportion to the release speed of the alcohol, and the faster the release speed of the alcohol is, the faster the stirring and mixing speed is, so that the adaptation of the alcohol release speed and the mixing speed is automatically realized, and the working efficiency is improved.

3. The heat exchange is effectively carried out in real time through the cooling water circulation consisting of the connecting pipe, the water pump and the water separator, and in the economic view, the heat exchange is carried out on the surface of the mixing bin in the upper annular cylindrical cavity by adopting a method of separating the upper annular cylindrical cavity from the lower annular cylindrical cavity, so that the full water submergence is not needed, the full surface covering can be carried out, the heat exchange speed is further accelerated by the flowing water, the temperature of the cooling water can be maintained in real time through the constructed cooling water circulation, the absorption of heat emitted when alcohol is mixed is accelerated, the active cooling is realized, the low-temperature environment in the cooling bin is maintained, and the activity of the pregnant mare serum gonadotropin is maintained.

Drawings

FIG. 1 is a schematic view of the internal structure of the cooling chamber of the present invention;

FIG. 2 is a schematic external view of the present invention;

FIG. 3 is a surface profile of a spin basket according to the present invention;

FIG. 4 is a sectional view showing the inner structure of the release cartridge according to the present invention;

FIG. 5 is an exploded view of the release cartridge of the present invention;

FIG. 6 is a schematic view of the inclined underside of the water separator of the present invention;

FIG. 7 is a schematic view of the structure of a spacing ring and a first blocking net of the present invention;

FIG. 8 is a partial cross-sectional view of a water knockout vessel and a mixing bin of the present invention;

FIG. 9 is an enlarged view of portion A of FIG. 1 in accordance with the present invention;

fig. 10 is an enlarged view of the portion B of fig. 8 according to the present invention.

In the figure: 1. a mixing bin; 2. a cooling bin; 21. a limiting ring; 3. a belt pulley transmission mechanism; 4. a rotary drum; 5. a release cartridge; 51. an outer cylinder; 52. a first through hole; 53. an inner barrel; 54. a second through hole; 55. a counterweight ball; 56. a spring; 57. a bottom plug; 58. a piston; 59. stirring blades; 6. helical leaves; 7. a water separator; 71. a snap ring; 72. a water outlet; 8. a first barrier net; 9. feeding ice water into the column; 10. a connecting pipe; 101. a second barrier net; 11. a water pump; 12. a top cover; 121. a first feed column; 122. a second feed column; 13. a funnel.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1 to 10, in the embodiment of the present invention, a rapid purification apparatus for pregnant mare serum gonadotropin comprises a mixing chamber 1, a cooling chamber 2, a belt pulley transmission mechanism 3, a rotary drum 4 and a funnel 13, wherein the rotary drum 4 is in transmission connection with the belt pulley transmission mechanism 3, the top of the mixing chamber 1 is further covered with a top cover 12, the funnel 13 is fixedly installed at the top of the top cover 12, a bottom output pipe of the funnel 13 is inserted downwards into the rotary drum 4 but is not in contact with the rotary drum 4, the mixing chamber 1 is placed in the middle of an inner cavity of the cooling chamber 2, the rotary drum 4 extends downwards into the inner cavity of the mixing chamber 1, seven release drums 5 are fixedly communicated with the outer surface of the bottom of the rotary drum 4 at equal intervals from top to bottom, the bottom of the rotary drum 4 is sealed and is not communicated, the seven release drums 5 are all located in the inner cavity of the mixing chamber 1, the release drums 5 comprises an outer cylinder 51 and a first through hole 52 opened on the surface of the outer cylinder 51, an inner cylinder 53 is sleeved in an inner cavity of the outer cylinder 51, a second through hole 54 is formed in the surface of the inner cylinder 53, the second through hole 54 and the first through hole 52 are dislocated in an initial state, a counterweight ball 55 is placed in the inner cavity of the inner cylinder 53, a bottom plug 57 is installed at an opening at the front end of the outer cylinder 51, a spring 56 is further arranged in the inner cavity of the outer cylinder 51, two ends of the spring 56 are respectively and elastically supported on the inner cylinder 53 and the bottom plug 57, when the spring 56 is subjected to the pressure of the inner cylinder 53 to reach the maximum compression amount, the second through hole 54 is aligned with the first through hole 52 in a superposition mode, and the inner cavity of the inner cylinder 53 is communicated with the outer environment of the outer cylinder 51 through the first through hole 52 and the second through hole 54.

Wherein, the tip opening part of inner tube 53 installs piston 58 and the inner wall laminating contact of urceolus 51, and the surface of urceolus 51 still is equipped with stirring leaf 59, has all seted up the fretwork groove on end plug 57 and the piston 58, and the fretwork groove of end plug 57 is for the outer space of intercommunication spring 56 place space and urceolus 51, avoids having sealed here air that brings and can't discharge, still receives the negative effects of atmospheric pressure when leading to inner tube 53 compression spring 56.

The stiffness coefficient of the spring 56 in the seven release cylinders 5 decreases in sequence from top to bottom, so that the liquid level rises with the injection of alcohol due to the raw material, alcohol and other solution moderators in the mixing bin 1, and the release cylinder 5 located relatively below bears the water pressure of a deeper water level, so that the pressure from the outside borne by the inner cylinder 53 located below is greater due to the combined action of the water pressure and the spring 56, and therefore, the elastic force of the spring 56 on the inner cylinder 53 below needs to be reduced, so that the total pressure borne by the inner cylinder 53 is negatively compensated.

Wherein, the inner cavity wall of the cooling bin 2 is provided with a spacing ring 21, the top of the spacing ring 21 is provided with a first blocking net 8, the inner ring of the first blocking net 8 is sleeved on the outer surface of the mixing bin 1, an annular columnar cavity is formed between the inner cavity of the cooling bin 2 and the outer wall of the mixing bin 1 and is divided into an upper annular columnar cavity and a lower annular columnar cavity by the first blocking net 8, because the density of the first barrier net 8 is less than that of water, when the water submerges the first barrier net 8, ice blocks can float along with the water, this can result in the water at the bottom of the lower annular cylindrical cavity being at a slightly higher temperature due to insufficient contact with the ice, the water pumped by the water pump 11 for cooling is pumped from the bottom of the lower annular cylindrical cavity, so that the ice blocks need to be blocked in the lower annular cylindrical cavity by the first blocking net 8, and the water pumped by the water pump 11 is cooled.

Wherein, be equipped with ice water mixture in the lower annular columnar cavity, the outer wall fixed mounting of cooling bin 2 has frozen water feeding column 9 and the bottom of frozen water feeding column 9 and the lower annular columnar cavity intercommunication of cooling bin 2, and the staff can directly drop the ice-cube at frozen water feeding column 9's open-top department to the water injection, ice-cube and water can enter into lower annular columnar cavity inside, including follow-up need add new ice-cube in the lower annular columnar cavity and all can add here.

Wherein, the outside of the cooling bin 2 is provided with a water pump 11, the lower annular columnar cavity of the cooling bin 2 is communicated with the water pump 11 through a connecting pipe 10, the inside of the connecting pipe 10 is provided with a second separation net 101, the second separation net 101 is used for blocking ice cubes and avoiding the phenomenon of pipeline blockage caused by the fact that the ice cubes are pumped away, the water pump 11 is communicated with a water separator 7 arranged at the top of the mixing bin 1 through a communicating pipe and pumps the water in the lower annular columnar cavity into the inner cavity of the water separator 7, the bottom of the water separator 7 is provided with a circle of water outlets 72 at equal intervals, the water flow flowing out of the water outlets 72 flows onto the surface of the cooling bin 2 and then flows down along the surface of the cooling bin 2, and due to the phenomenon that the water flow is diffused on a curved surface and a flat plate, even if only the position of the water outlet 72 is arranged at the bottom of the water separator 7 can leak water, but after the water flow flowing out of each water outlet 72 flows down along the surface of the cooling bin 2, the coalescence is still formed due to the diffusion phenomenon, so that the outer surface of the cooling compartment 2 is completely covered.

Wherein, the top edge of mixing storehouse 1 is the fillet design, water in the inner chamber of water knockout drum 7 flows down to the fillet of mixing storehouse 1 outside edge and flows down along the surface of mixing storehouse 1 through outlet 72, the inner ring bottom of water knockout drum 7 still is equipped with block in the inboard snap ring 71 in mixing storehouse 1 top, the fillet design can be avoided water to take place the sputtering after falling on mixing storehouse 1, make the flow of water more natural level and smooth, it is impure to avoid flying the water that splashes probably to fall to the supernatant that leads to finally making in the inner chamber of mixing storehouse 1, the rivers that flow down can directly take away the heat that produces on mixing storehouse 1, raw materials and external heat exchange rate in the mixing storehouse 1 with higher speed.

Wherein, the surface of a rotatory section of thick bamboo 4 still is equipped with spiral leaf 6, and spiral leaf 6 can play the effect of an auxiliary stirring, as shown in fig. 3, when a rotatory section of thick bamboo 4 drove 6 counter-clockwise turning of spiral leaf, spiral leaf 6 can upwards stir the raw materials in 1 inner chambers of mixing bunker to accelerate the diffusion velocity of inside alcohol in the raw materials.

The number of the first through holes 52 formed in the surface of the outer cylinder 51 is at least five and at most eight, the opening directions of the first through holes 52 are different, the number of the second through holes 54 formed in the inner cylinder 53 is equal to the number of the first through holes 52, namely, each first through hole 52 needs one second through hole 54 with the same opening direction to coincide with the first through hole, the inner cavity of the inner cylinder 53 is communicated with the outer space of the outer cylinder 51, the opening directions of the first through holes 52 are different, and the purpose of diffusing the alcohol discharged from the inner cavity of the inner cylinder 53 to all directions of the inner cavity of the mixing bin 1 is achieved.

Wherein, the top of top cap 12 still is equipped with a feeding column 121 and a feeding column 122 No. two, and the bottom opening of a feeding column 121 and a feeding column 122 No. two all faces the inner chamber of mixing bin 1, can directly pour into raw materials and other reagents in the inner chamber of mixing bin 1 from a feeding column 121 and a feeding column 122 No. two.

The working principle and the using process are as follows:

putting ice blocks into an opening at the top of the ice water feeding column 9, then injecting water to enable an ice water mixture to fill the lower annular cylindrical cavity, starting the water pump 11 to take water from the lower shape-changing cylindrical cavity, sending the water into the water distributor 7, sleeping to flow out along the water outlet 72 and cover the outer surface of the cooling bin 2, cooling the cooling bin 2, and finally enabling cold water to pass through the first blocking net 8 along the surface of the cooling bin 2 and return to the lower annular cylindrical cavity again to be cooled again to form cooling water circulation;

injecting pregnant mare serum raw materials into the mixing bin 1, injecting metaphosphoric acid for adsorption, then injecting chilled ninety-five percent concentration alcohol into the rotating drum 4 from the hopper 13, controlling the belt pulley transmission mechanism 3 to drive the rotating drum 4 to rotate, wherein the alcohol enters the rotating drum 4 and is thrown into the inner cavity of the inner drum 53 due to centrifugal tendency, and as the rotating drum 4 continuously rotates, the counterweight ball 55 is pressed against the end part of the inner drum 53 due to centrifugal tendency and pushes the inner drum 53 outwards, the inner drum 53 moves and compresses the spring 56, and the inner drum 53 enables the second through hole 54 and the first through hole 52 to coincide in the moving process, the inner drum 53 is communicated with the outer space of the outer drum 51, namely, the alcohol can flow into the pregnant mare serum of the cooling bin 2 through the second through hole 54 and the first through hole 52, and as the rotating drum 4 continuously rotates, the stirring blades 59 on the surfaces of the releasing drum 5 body, the outer drum 51 and the spiral blades 6 on the surfaces of the rotating drum 4 are continuously and continuously Stirring the alcohol and the serum during the rotation to enable the alcohol to be quickly diffused in the serum, wherein the orientations of the first through holes 52 are different, and the directions of the output of the alcohol are different, so that the alcohol is not added in one place of the serum, and the output port of the alcohol can be continuously changed along with the rotation, the problem that the local concentration of the alcohol is too high is solved, and the quick and uniform diffusion of the alcohol is realized through the real-time stirring;

the cooling water circulation formed by the connecting pipe 10, the water pump 11 and the water separator 7 can effectively carry out heat exchange in real time, and in the economic view, the method of separating the upper annular columnar cavity from the lower annular columnar cavity is adopted, so that the surface of the mixing bin 1 in the upper annular columnar cavity can carry out heat exchange through flowing water, the purpose of not submerging the mixing bin with the whole surface can be achieved, the flowing water further accelerates the heat exchange speed, and the constructed cooling water circulation can maintain the temperature of the cooling water in real time.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a quick purification device for pregnant horse serum gonadotropin, includes mixing storehouse (1), cooling bin (2), belt pulley drive mechanism (3), rotatory section of thick bamboo (4) and funnel (13), rotatory section of thick bamboo (4) are connected with belt pulley drive mechanism (3) transmission, the top of mixing storehouse (1) is still covered and is closed top cap (12), funnel (13) fixed mounting insert in rotatory section of thick bamboo (4) down but with rotatory section of thick bamboo (4) contactless in the top of top cap (12) and the bottom output tube of funnel (13), mix storehouse (1) and place in the inner chamber middle part of cooling storehouse (2) and rotatory section of thick bamboo (4) downwardly extending to the inner chamber of mixing storehouse (1), its characterized in that: the outer surface of the bottom of the rotating cylinder (4) is fixedly communicated with seven releasing cylinders (5) at equal intervals from top to bottom, the bottom of the rotating cylinder (4) is sealed and does not flow, the seven releasing cylinders (5) are located in the inner cavity of the mixing bin (1), each releasing cylinder (5) comprises an outer cylinder (51) and a first through hole (52) formed in the surface of the outer cylinder (51), an inner cylinder (53) is sleeved in the inner cavity of the outer cylinder (51), a second through hole (54) is formed in the surface of the inner cylinder (53), the second through hole (54) and the first through hole (52) are staggered in an initial state, a counterweight ball (55) is placed in the inner cavity of the inner cylinder (53), a bottom plug (57) is installed at an opening at the front end of the outer cylinder (51), a spring (56) is further arranged in the inner cavity of the outer cylinder (51), and two ends of the spring (56) are respectively and elastically supported on the inner cylinder (53) and the bottom plug (57), when the spring (56) is pressed by the inner cylinder (53) to reach the maximum compression amount, the second through hole (54) is overlapped and aligned with the first through hole (52) and the inner cavity of the inner cylinder (53) is communicated with the outer environment of the outer cylinder (51) through the first through hole (52) and the second through hole (54).

2. The device for rapidly purifying pregnant mare serum gonadotropin as claimed in claim 1, wherein: the inner wall laminating contact of piston (58) and urceolus (51) is installed to the tip opening part of inner tube (53), the surface of urceolus (51) still is equipped with stirring leaf (59), all seted up the fretwork groove on end stopper (57) and piston (58).

3. The device for rapidly purifying pregnant mare serum gonadotropin as claimed in claim 1, wherein: the stiffness coefficients of the springs (56) in the seven release barrels (5) are decreased from top to bottom in sequence.

4. The device for rapidly purifying pregnant mare serum gonadotropin as claimed in claim 1, wherein: the inner chamber wall of cooling storehouse (2) is equipped with spacing ring (21), settle the top of spacing ring (21) has a barrier net (8), the surface that mixes storehouse (1) is located to the inner ring cover of a barrier net (8), form annular column cavity and this annular column cavity through a barrier net (8) and be separated into annular column cavity and lower annular column cavity two parts between the inner chamber of cooling storehouse (2) and the outer wall of mixing storehouse (1).

5. The device for rapidly purifying pregnant mare serum gonadotropins as claimed in claim 4, wherein: an ice water mixture is filled in the lower annular columnar cavity, an ice water feeding column (9) is fixedly installed on the outer wall of the cooling bin (2), and the bottom of the ice water feeding column (9) is communicated with the lower annular columnar cavity of the cooling bin (2).

6. The device for rapidly purifying pregnant mare serum gonadotropin as claimed in claim 1, wherein: the outside in cooling bin (2) is equipped with water pump (11), the lower annular column cavity in cooling bin (2) passes through connecting pipe (10) and water pump (11) intercommunication, there are No. two in the inside of connecting pipe (10) to block net (101), water pump (11) through communicating pipe with install in water knockout drum (7) the intercommunication at mixed storehouse (1) top and will descend the water pump in the annular column cavity to deliver to the inner chamber of water knockout drum (7), round outlet (72) have been seted up to the bottom equidistance of water knockout drum (7).

7. The device for rapidly purifying pregnant mare serum gonadotropins as claimed in claim 6, wherein: the edge of the top of the mixing bin (1) is designed to be a round angle, water in the inner cavity of the water separator (7) flows down to the round angle of the outer edge of the mixing bin (1) through the water outlet (72) and flows down along the outer surface of the mixing bin (1), and a snap ring (71) clamped on the inner side of the top of the mixing bin (1) is further arranged at the bottom of the inner ring of the water separator (7).

8. The device for rapidly purifying pregnant mare serum gonadotropin as claimed in claim 1, wherein: the outer surface of the rotary cylinder (4) is also provided with spiral blades (6).

9. The device for rapidly purifying pregnant mare serum gonadotropin as claimed in claim 2, wherein: the number of the first through holes (52) formed in the surface of the outer cylinder (51) is at least five and at most eight, the opening directions of the first through holes (52) are different, and the number of the second through holes (54) formed in the inner cylinder (53) is equal to the number of the first through holes (52).

10. The device for rapidly purifying pregnant mare serum gonadotropin as claimed in claim 1, wherein: the top of top cap (12) still is equipped with a feeding column (121) and No. two feeding columns (122), the bottom opening of a feeding column (121) and No. two feeding columns (122) all is towards the inner chamber of mixing storehouse (1).