CN113444628A - Tissue block cleaning device and cleaning method thereof - Google Patents

Tissue block cleaning device and cleaning method thereof Download PDF

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Publication number
CN113444628A
CN113444628A CN202110588542.5A CN202110588542A CN113444628A CN 113444628 A CN113444628 A CN 113444628A CN 202110588542 A CN202110588542 A CN 202110588542A CN 113444628 A CN113444628 A CN 113444628A
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liquid
cleaning
filter screen
tube
turbidity
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CN113444628B (en
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杨鵾
乔聃
陶小东
王树恒
周红
汪新艳
张安英
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University of Electronic Science and Technology of China
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University of Electronic Science and Technology of China
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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    • C12M23/02—Form or structure of the vessel
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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Abstract

The invention relates to the technical field of cleaning devices, in particular to a tissue block cleaning device and a cleaning method thereof.

Description

Tissue block cleaning device and cleaning method thereof
Technical Field
The invention relates to the technical field of cleaning devices, in particular to a tissue mass cleaning device and a cleaning method thereof.
Background
In present common experimental procedure, organize the piece and wash and mostly adopt manual abluent method, be about to organize the piece and put into the centrifuging tube, add the washing liquid, absorb the air with the help of the liquid-transfering gun and pour into and blow the washing liquid and the repetitive operation who soaks the tissue piece to the realization is to the cleanness of organizing the piece, and long-time manual operation can cause the cost of labor higher, and efficiency on the low side, is unfavorable for going on of synchronous experiment.
Disclosure of Invention
It is an object of the present invention to provide a tissue mass washing device that ameliorates the above problems. In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
on the one hand, this application provides an organize piece belt cleaning device, including baffle, fixture and wiper mechanism, fixture sets up the lower surface of baffle, fixture is used for the centre gripping centrifuging tube, the wiper mechanism sets up the top of baffle, wiper mechanism is used for wasing organize the piece in the centrifuging tube.
Preferably, the cleaning mechanism includes a liquid supply mechanism, a sterilization mechanism, a separation mechanism and a liquid discharge mechanism, the liquid supply mechanism is used for conveying a cleaning liquid into the centrifuge tube, the sterilization mechanism is used for mixing the cleaning liquid and the tissue mass in the centrifuge tube, the separation mechanism is used for separating the cleaning liquid and the tissue mass from each other in the centrifuge tube, and the liquid discharge mechanism is used for extracting the cleaning liquid in the centrifuge tube.
Preferably, the liquid supply mechanism includes second liquid collecting bucket, first water pump and feed liquor pipe, the second liquid collecting bucket pass through the hose with the water inlet of first water pump is connected, the delivery port of first water pump pass through the hose with the feed liquor union coupling, the feed liquor pipe sets up the mouth of pipe top of centrifuging tube.
Preferably, separating mechanism includes flexible motor, hollow tube and filter screen, flexible motor flexible end with the top of hollow tube is connected, the bottom downwardly extending of hollow tube extends in the centrifuge tube, the bottom of hollow tube with filter screen fixed connection, be provided with the slip opening that is used for preventing that the intake pipe from producing the maloperation on the lateral wall of hollow tube, be provided with on the filter screen and be used for preventing the intake pipe produces the through-hole of maloperation.
Preferably, degerming mechanism includes air pump and intake pipe, the gas outlet of air pump pass through the hose with intake-tube connection, the intake pipe is the L type, the vertical section setting of intake pipe is in the hollow tube, just the through-hole of setting on the filter screen is passed to the bottom of vertical section, and downwardly extending extremely the inner chamber bottom of centrifuging tube, the horizontal segment of intake pipe passes the setting and is in slip opening on the hollow tube with the top of vertical section is connected, just the intake pipe with the relative position of centrifuging tube is fixed.
Preferably, a plurality of air outlet holes are arranged on the side wall of the vertical section of the centrifugal tube.
Preferably, drainage mechanism includes first liquid collecting bucket, second water pump and imbibition gravity ball, first liquid collecting bucket with the delivery port of second water pump passes through the hose connection, the water inlet of second water pump with imbibition gravity ball passes through the hose connection, imbibition gravity ball sets up the upper surface at the filter screen, and follows the filter screen removes.
Preferably, an optical detection mechanism is arranged on the lower surface of the clamping mechanism and used for detecting the turbidity of the cleaning liquid in the centrifugal pipe.
Preferably, a sterilization mechanism is arranged above the partition board and is used for sterilizing and disinfecting the area above the partition board.
Preferably, the upper surface of baffle is provided with the box, wiper mechanism sets up in the last box, be provided with the window on the lateral wall of going up the box, be provided with control module on the lateral wall of going up the box, control module is used for controlling wiper mechanism washs the tissue piece in the centrifuge tube.
Preferably, the control module comprises a processing unit, a display unit, an adjusting unit and a detection unit;
the processing unit is used for receiving and processing the monitoring numerical value sent by the detection unit and sending a corresponding control instruction to the cleaning mechanism according to the monitoring numerical value, and the control instruction is used for controlling the cleaning mechanism to clean the tissue block in the centrifugal tube;
the display unit is used for displaying the air inlet intensity, the air inlet frequency, the number of cycles to be cleaned and the operation duration;
the adjusting unit comprises an emergency interruption button, a disinfection button, an adjusting button, an execution button and a pipeline cleaning button;
the detection unit comprises a second hydraulic sensor arranged at the bottom of the second liquid collecting barrel, a first hydraulic sensor arranged at the bottom of the first liquid collecting barrel, a flow sensor arranged on the first water pump and an optical sensor arranged below the partition plate.
In a second aspect, the present application provides a cleaning method based on the tissue mass cleaning device, including:
screwing a clamping knob on the clamping mechanism to enable two arc clamping plates on the clamping mechanism to move away from each other, so that a clamping hole on the clamping mechanism is enlarged, and when the diameter of the clamping hole is larger than the diameter of a pipe orifice of a centrifugal pipe, stopping screwing the clamping knob;
placing the centrifuge tube filled with the tissue block to be cleaned in the clamping hole, and screwing the clamping knob to enable the two arc clamping plates on the clamping mechanism to move towards each other so as to clamp the outer wall of the centrifuge tube, and stopping screwing the clamping knob when the centrifuge tube is fixed on the two arc clamping plates;
selecting the single washing liquid consumption corresponding to the current specification of the centrifugal tube through an adjusting button, and then clicking an executing button to enable the cleaning mechanism to execute cleaning operation until the cleaning mechanism stops running, wherein at the moment, a display unit displays cleaning completion information;
an operator twists a clamping knob on the clamping mechanism to enable two arc clamping plates on the clamping mechanism to loosen the centrifugal tube, and then the centrifugal tube is taken out.
Preferably, clicking the execution button to enable the cleaning mechanism to execute the cleaning operation until the cleaning mechanism stops operating includes:
resetting operation: starting a telescopic motor to enable the telescopic motor to drive a hollow pipe to move up and down, and further enable the hollow pipe to drive a filter screen to reach an initial preset height, wherein the filter screen is located at the middle-upper part of the centrifuge pipe, and the initial preset height is the highest station of the filter screen;
liquid feeding operation: starting a first water pump, enabling the first water pump to convey cleaning liquid in a second liquid collecting barrel to a liquid inlet pipe through a hose, enabling the cleaning liquid to enter the centrifugal pipe, and stopping running the first water pump when a flow sensor detects that the cleaning liquid dropping into the centrifugal pipe reaches preset single-time cleaning liquid consumption;
and (3) sterilization operation: starting an air pump, leading the air pump to introduce air into an air inlet pipe, further leading the tissue blocks in the centrifugal pipe to be fully mixed with the cleaning fluid under the action of air bubble floating, and stopping running the air pump after the air introduction time reaches a first preset time;
and (3) filtering operation: starting the telescopic motor to enable the telescopic motor to drive the hollow pipe to move downwards so as to enable the filter screen to move downwards, stopping running the telescopic motor when the filter screen reaches a preset low position, and separating the tissue block from the cleaning solution, wherein the preset low position is the lowest station of the filter screen;
detection operation: starting an optical sensor, enabling the optical sensor to detect the current turbidity of the cleaning solution above the tissue block, uploading the current turbidity to a processing unit, and recording the current turbidity as a first turbidity;
liquid drainage operation: starting a second water pump, pumping the cleaning solution above the filter screen into a first liquid collecting barrel through a liquid suction gravity ball and the hose by the second water pump, simultaneously starting the optical sensor to detect the turbidity of the centrifuge tube above the filter screen, and stopping running the second water pump when the turbidity is smaller than or equal to a third turbidity, wherein the third turbidity is the turbidity of the centrifuge tube when the cleaning solution is not above the filter screen;
executing the reset operation;
the processing unit compares the first turbidity with a first preset turbidity, and if the first turbidity is greater than or equal to the first preset turbidity, the liquid feeding operation, the sterilizing operation, the filtering operation, the detecting operation, the liquid discharging operation and the resetting operation are sequentially and repeatedly executed;
and if the first turbidity is smaller than the first preset turbidity, sending a finishing instruction to the display unit, and then starting the sterilization mechanism, wherein the finishing instruction is an instruction for enabling the display unit to display text information prompting an operator to finish cleaning.
The invention has the beneficial effects that:
according to the invention, the tissue block is automatically cleaned by the cleaning mechanism, so that the existing pure manual cleaning mode is replaced, the labor cost is greatly reduced, and the experiment efficiency is improved.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the embodiments of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a first state diagram of a tissue mass washing device according to an embodiment of the present invention.
FIG. 2 is a second state diagram of the tissue mass cleaning device according to the embodiment of the present invention.
Fig. 3 is a schematic structural diagram of a clamping mechanism according to an embodiment of the invention.
Fig. 4 is an assembly view of a clamping mechanism and a centrifuge tube according to an embodiment of the invention.
Fig. 5 is a schematic structural diagram of a tissue mass cleaning device according to an embodiment of the present invention.
The labels in the figure are: 11-a first liquid collecting barrel, 12-a second liquid collecting barrel, 13-a telescopic motor, 14-a first water pump, 15-a second water pump, 16-a liquid inlet pipe, 17-an air inlet pipe, 18-a liquid absorption gravity ball, 19-a filter screen, 110-a hollow pipe, 111-a sliding opening, 2-a partition plate, 3-a clamping mechanism, 4-an optical sensor, 5-a centrifugal pipe, 6-an ultraviolet lamp, 7-an upper box body, 71-a window and 8-a control module.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures. Meanwhile, in the description of the present invention, the terms "first", "second", and the like are used only for distinguishing the description, and are not to be construed as indicating or implying relative importance.
Example 1
As shown in fig. 1-5, this embodiment provides a tissue mass belt cleaning device, including baffle 2, fixture 3 and wiper mechanism, fixture 3 sets up the lower surface of baffle 2, fixture 3 is used for centre gripping centrifuging tube 5, the wiper mechanism sets up the top of baffle 2, wiper mechanism is used for wasing the tissue mass in the centrifuging tube 5.
The cleaning mechanism comprises a liquid supply mechanism, a sterilization mechanism, a separation mechanism and a liquid discharge mechanism;
the liquid supply mechanism comprises a second liquid collecting barrel 12, a first water pump 14 and a liquid inlet pipe 16, a second pressure sensor is further arranged at the bottom of the second liquid collecting barrel 12 and used for detecting whether cleaning liquid in the second liquid collecting barrel 12 is sufficient or not, and if the pressure value of the second pressure sensor is lower than the lowest threshold value, the operation of the cleaning mechanism is stopped;
meanwhile, a flow meter is arranged on the first water pump 14 and is used for detecting the amount of the cleaning liquid which is conveyed into the centrifuge tube 5 by the first water pump 14 in a single time, so that the control unit can accurately control the start-stop time of the first water pump 14;
meanwhile, the liquid inlet pipe 16 should be located above the centrifuge pipe 5, and the highest water level of the cleaning liquid dropped into the centrifuge pipe 5 should be lower than the bottom of the liquid inlet pipe 16 to prevent the liquid inlet pipe 16 from being polluted, and meanwhile, the bottom of the liquid inlet pipe 16 should be located above the highest station of the filter screen 19 to prevent the bottom of the liquid inlet pipe 16 from contacting the filter screen 19 and further polluting the liquid inlet pipe 16;
when the filter screen 19 is at the highest working position, the liquid level of the cleaning liquid should be lower than the filter screen 19, so that the gas in the gas inlet pipe 17 can be discharged from the centrifuge tube 5; because the washing liquid is comparatively thick, if when the degerming operation, filter screen 19 soaks in the washing liquid, the bubble of filter screen 19 below this moment can be after passing through filter screen 19, produces a large amount of bubbles, and then probably leads to the washing liquid to spill over centrifuging tube 5.
The degerming mechanism comprises an air pump and an air inlet pipe 17, and the separating mechanism comprises a telescopic motor 13, a hollow pipe 110 and a filter screen 19; the vertical section of the air inlet pipe 17 is arranged in the hollow pipe 110, the horizontal section of the air inlet pipe 17 penetrates through a sliding opening 111 on the side wall of the hollow pipe 110, and the sliding opening 111 can enable the position of the air inlet pipe 17 to be always kept unchanged when the hollow pipe 110 drives the filter screen 19 to move up and down;
as shown in fig. 2, when initial state, filter screen 19 is located the well upper portion of centrifuging tube 5, this moment filter screen 19 is located highest station, this moment the most of the vertical section of intake pipe 17 is located filter screen 19's below, through the air pump with intake pipe 17 to let in mist in centrifuging tube 5, preferably, be provided with a plurality of air vents on the lateral wall of the vertical section of intake pipe 17, make tissue piece and the washing liquid in centrifuging tube 5 can mix more fully.
In a specific embodiment of the present disclosure, the cleaning mechanism includes a liquid supply mechanism, a sterilization mechanism, a separation mechanism, and a liquid discharge mechanism, the liquid supply mechanism is configured to deliver a cleaning liquid into the centrifuge tube 5, the sterilization mechanism is configured to mix the cleaning liquid and the tissue block in the centrifuge tube 5, the separation mechanism is configured to separate the cleaning liquid and the tissue block in the centrifuge tube 5 from each other, and the liquid discharge mechanism is configured to extract the cleaning liquid in the centrifuge tube 5;
next, the liquid discharge mechanism includes a first liquid collecting bucket 11, a second water pump 15 and a liquid suction gravity ball 18, as shown in fig. 1, after the tissue mass is thoroughly mixed with the cleansing fluid under the action of the high-velocity air flow passing through said air inlet duct 17, the sieve 19 is moved downwards by the hollow tube 110 to the position shown in the figure, where the tissue mass is collected below the sieve 19, the washing liquid is collected above the sieve 19, then the second water pump 15 is started to pump out the cleaning liquid, at the moment, the pumping-out state of the cleaning liquid is monitored in real time through the optical sensor 4, i.e. the optical sensor 4 detects the current turbidity of the washing liquid above the tissue mass, and if the current turbidity is less than or equal to a third turbidity, when the cleaning liquid above the filter screen 19 is judged to be completely discharged out of the centrifuge tube 5, the second water pump 15 is stopped to operate;
secondly, the optical sensor comprises a first gear and a second gear, the first gear is used for detecting the turbidity of a section of liquid column of the cleaning liquid in the test tube and above the filter screen 19, the focus point of the optical sensor is a section of liquid column with a preset height, the second gear is used for detecting the turbidity of a section of liquid column with a height of 1-2 centimeters above the upper surface of the filter screen 19, the first gear is used for executing the detection operation, and the second gear is used for executing the liquid discharge operation.
Meanwhile, the optical sensor 4 is also used for monitoring the turbidity of the cleaning liquid after each mixed cleaning, further judging whether the cleaning of the tissue block reaches the preset cleanliness, and further judging whether the cleaning is required to be repeated again until the cleanliness of the tissue block reaches the standard;
meanwhile, the bottom of the first liquid collecting barrel 11 is also provided with a first pressure sensor for detecting the amount of the cleaning waste liquid in the first liquid collecting barrel 11, and if the pressure value of the first pressure sensor is greater than the highest threshold value, the operation of the cleaning mechanism is stopped.
According to the specific embodiment of the disclosure, the tissue block is automatically cleaned by the cleaning mechanism, so that the existing pure manual cleaning mode is replaced, the labor cost is greatly reduced, and the experiment efficiency is improved.
In one embodiment of the present disclosure, the liquid supply mechanism includes a second liquid collecting barrel 12, a first water pump 14 and a liquid inlet pipe 16, the second liquid collecting barrel 12 is connected to a water inlet of the first water pump 14 through a hose, a water outlet of the first water pump 14 is connected to the liquid inlet pipe 16 through a hose, and the liquid inlet pipe 16 is disposed above a nozzle of the centrifugal tube 5; the highest water level of the cleaning liquid dripped into the centrifugal tube 5 is lower than the bottom of the liquid inlet tube 16, so that the liquid inlet tube 16 is prevented from being polluted; meanwhile, a flow meter is arranged on the first water pump 14 and used for detecting the amount of the cleaning liquid which is conveyed by the first water pump 14 into the centrifugal tube 5 in a single time, and therefore the control unit can accurately control the starting and stopping time of the first water pump 14.
In one embodiment of the present disclosure, the separating mechanism comprises a telescopic motor 13, a hollow tube 110 and a filter screen 19, the telescopic end of the telescopic motor 13 is connected with the top of the hollow tube 110, the bottom of the hollow tube 110 extends downwards into the centrifuge tube 5, the bottom of the hollow tube 110 is fixedly connected with the filter screen 19, the side wall of the hollow tube 110 is provided with a sliding opening 111 for preventing the air inlet pipe 17 from being operated by mistake, the filter screen 19 is provided with a through hole for preventing the air inlet pipe 17 from generating misoperation, the vertical section of the air inlet pipe 17 is arranged in the hollow pipe 110, the horizontal section of the air inlet pipe 17 passes through the sliding opening 111 on the side wall of the hollow pipe 110, the position of the air inlet pipe 17 can be kept unchanged by the sliding opening 111 when the hollow pipe 110 drives the filter screen 19 to move up and down.
In a specific embodiment of the present disclosure, the sterilization mechanism includes an air pump and an air inlet pipe 17, an air outlet of the air pump is connected to the air inlet pipe 17 through a hose, the air inlet pipe 17 is L-shaped, a vertical section of the air inlet pipe 17 is disposed in the hollow pipe 110, a bottom of the vertical section passes through a through hole disposed on the filter screen 19 and extends downward to a bottom of an inner cavity of the centrifuge tube 5, a horizontal section of the air inlet pipe 17 passes through a sliding opening disposed on the hollow pipe 110 and is connected to a top of the vertical section, and a relative position of the air inlet pipe 17 and the centrifuge tube 5 is fixed; the vertical section of the air inlet pipe 17 is arranged in the hollow pipe 110, the horizontal section of the air inlet pipe 17 penetrates through a sliding opening 111 on the side wall of the hollow pipe 110, and the sliding opening 111 can enable the position of the air inlet pipe 17 to be always kept unchanged when the hollow pipe 110 drives the filter screen 19 to move up and down;
as shown in fig. 2, when initial state, filter screen 19 is located the well upper portion of centrifuging tube 5, this moment filter screen 19 is located highest station, this moment the most of the vertical section of intake pipe 17 is located filter screen 19's below, through the air pump with intake pipe 17 to let in mist in centrifuging tube 5, preferably, be provided with a plurality of air vents on the lateral wall of the vertical section of intake pipe 17, make tissue piece and the washing liquid in centrifuging tube 5 can mix more fully.
In a specific embodiment of this disclosure, be provided with a plurality of ventholes on the lateral wall of the vertical section of centrifuging tube 5, make tissue piece and the washing liquid in centrifuging tube 5 can mix more fully.
In a specific embodiment of this disclosure, drainage mechanism includes first liquid collecting bucket 11, second water pump 15 and imbibition gravity ball 18, first liquid collecting bucket 11 with the delivery port of second water pump 15 passes through the hose connection, the water inlet of second water pump 15 with imbibition gravity ball 18 passes through the hose connection, imbibition gravity ball 18 sets up the upper surface at filter screen 19, and follows filter screen 19 removes.
In one embodiment of the present disclosure, an optical detection mechanism is disposed on a lower surface of the clamping mechanism 3, and the optical detection mechanism is used for detecting the turbidity of the cleaning solution in the centrifugal tube 5.
As shown in fig. 1, after the tissue mass and the cleaning solution are sufficiently mixed by the high-speed airflow passing through the air inlet pipe 17, the filter screen 19 moves down to the position shown in the figure by the hollow pipe 110, the tissue mass is concentrated below the filter screen 19, the cleaning solution is concentrated above the filter screen 19, then the second water pump 15 is started to pump out the cleaning solution, at this time, the pumping-out state of the cleaning solution is monitored in real time by the optical sensor 4, that is, the optical sensor 4 detects the current turbidity of the cleaning solution located above the tissue mass, and if the current turbidity is less than or equal to the third turbidity, it is determined that all the cleaning solution located above the filter screen 19 has been discharged out of the centrifuge tube 5, and then the second water pump 15 is stopped.
Meanwhile, the optical sensor 4 is also used for monitoring the turbidity of the cleaning liquid after each mixed cleaning, further judging whether the cleaning of the tissue block reaches the preset cleanliness, and further judging whether the cleaning is required to be repeated again until the cleanliness of the tissue block reaches the standard;
meanwhile, the bottom of the first liquid collecting barrel 11 is also provided with a first pressure sensor for detecting the amount of the cleaning waste liquid in the first liquid collecting barrel 11, and if the pressure value of the second pressure sensor is greater than the highest threshold value, the operation of the cleaning mechanism is stopped.
In one embodiment of the present disclosure, a sterilization mechanism is disposed above the partition board 2, and the sterilization mechanism is used for sterilizing and disinfecting the area above the partition board 2, and preferably, the sterilization mechanism may be an ultraviolet lamp 6.
In a specific embodiment of this disclosure, the upper surface of baffle 2 is provided with box 7, wiper mechanism sets up in going up box 7, be provided with window 71 on going up the lateral wall of box 7, be provided with control module 8 on going up the lateral wall of box 7, control module 8 is used for control wiper mechanism washs tissue piece in the centrifuging tube 5.
In one embodiment of the present disclosure, the control module 8 includes a processing unit, a display unit, an adjusting unit, and a detecting unit;
the processing unit is used for receiving and processing the monitoring numerical value sent by the detection unit and sending a corresponding control instruction to the cleaning mechanism according to the monitoring numerical value, and the control instruction is used for controlling the cleaning mechanism to clean the tissue blocks in the centrifuge tube 5;
the display unit is used for displaying the air inlet intensity, the air inlet frequency, the number of cycles to be cleaned and the operation duration;
the adjusting unit comprises an emergency interruption button, a disinfection button, an adjusting button, an execution button and a pipeline cleaning button;
the pipeline cleaning button is used for starting the cleaning mechanism to clean the centrifuge tube 5 which is newly put in, and the emergency interruption button is used for forcibly stopping the operation of the tissue block cleaning device.
The detection unit comprises a second hydraulic sensor arranged at the bottom of the second liquid collecting barrel 12, a first hydraulic sensor arranged at the bottom of the first liquid collecting barrel 11, a flow sensor arranged on the first water pump 14 and an optical sensor 4 arranged below the partition plate 2.
Example 2
Based on the tissue mass cleaning device in embodiment 1, this embodiment provides a cleaning method based on the tissue mass cleaning device, including:
s1, screwing a clamping knob 31 on a clamping mechanism 3 to enable two arc clamping plates 32 on the clamping mechanism 3 to move away from each other, so that a clamping hole on the clamping mechanism 3 is enlarged, and when the diameter of the clamping hole is larger than the diameter of a pipe orifice of a centrifugal pipe 5, stopping screwing the clamping knob 31;
s2, placing the centrifuge tube 5 filled with the tissue block to be cleaned in the clamping hole, and screwing the clamping knob 31 to enable the two arc clamping plates 32 on the clamping mechanism 3 to move towards each other so as to clamp the outer wall of the centrifuge tube 5, and stopping screwing the clamping knob 31 when the centrifuge tube 5 is fixed on the two arc clamping plates 32;
s3, selecting the single washing liquid consumption corresponding to the current specification of the centrifugal tube 5 through an adjusting button, clicking an executing button to enable the cleaning mechanism to execute cleaning operation until the cleaning mechanism stops running, and displaying cleaning completion information by a display unit at the moment;
s4, an operator twists the clamping knob 31 on the clamping mechanism 3 to enable the two arc clamping plates 32 on the clamping mechanism 3 to loosen the centrifugal tube 5, and then the centrifugal tube 5 is taken out.
In a specific embodiment of the present disclosure, the step S3 includes:
step S31, resetting operation: starting a telescopic motor 13, enabling the telescopic motor 13 to drive a hollow tube 110 to move up and down, further enabling the hollow tube 110 to drive a filter screen 19 to reach an initial preset height, enabling the filter screen 19 to be located at the middle-upper part of the centrifugal tube 5, and enabling the filter screen 19 to be located at the highest station;
step S32, liquid inlet operation: starting a first water pump 14, enabling the first water pump 14 to convey the cleaning liquid in the second liquid collecting barrel 12 to a liquid inlet pipe 16 through a hose, enabling the cleaning liquid to enter the centrifugal tube 5, and stopping running the first water pump 14 when a flow sensor detects that the cleaning liquid dropping into the centrifugal tube 5 reaches a preset single cleaning liquid consumption amount;
step S33, sterilization operation: starting an air pump, leading the air pump to introduce air into an air inlet pipe 17, further leading the tissue blocks in the centrifugal pipe 5 and the cleaning liquid to be fully mixed under the action of air bubble floating, and stopping running the air pump after the air introducing time reaches a first preset time;
wherein, in the degerming operation further comprising:
s331, starting an air pump, introducing an initial constant-pressure air flow into the centrifugal tube 5, and stopping introducing the initial constant-pressure air flow after a second preset time period;
s332, detecting the current turbidity of the cleaning solution in the centrifuge tube 5 through the optical sensor 4, and recording as a fifth turbidity;
s333, selecting a preset air inlet intensity and an air inlet frequency corresponding to the fifth turbidity according to the fifth turbidity;
and S334, introducing airflow into the centrifugal tube 5 according to the preset air inlet strength and the preset air inlet frequency. Step S34, filtering operation: starting the telescopic motor 13, enabling the telescopic motor 13 to drive the hollow tube 110 to move downwards, further enabling the filter screen 19 to move downwards, and stopping running the telescopic motor 13 when the filter screen 19 reaches a preset low position, wherein the tissue blocks are separated from the cleaning solution; step S35, detection operation: starting an optical sensor 4, enabling the optical sensor 4 to detect the current turbidity of the cleaning solution above the tissue block, uploading the current turbidity to a processing unit, and recording the current turbidity as a first turbidity;
step S36, liquid drainage operation: starting a second water pump 15, wherein the second water pump 15 pumps the cleaning solution above the filter screen 19 into a first liquid collecting barrel 11 through a liquid suction gravity ball 18 and the hose, and simultaneously starting the optical sensor 4 to detect the turbidity of the centrifugal tube 5 above the filter screen 19, and when the turbidity is less than or equal to a third turbidity, stopping running the second water pump 15, wherein the third turbidity is the turbidity of the centrifugal tube 5 when the cleaning solution is not above the filter screen 19;
next, in an embodiment of the present disclosure, the step s36 of performing a liquid discharge operation may further include: setting a second flow meter on the second water pump 15, presetting a drainage threshold value in the control unit, starting the second water pump 15 when the drainage operation is executed, detecting the drainage quantity through the second flow meter, and stopping running the second water pump 15 if the drainage quantity is greater than or equal to the drainage threshold value;
s37, executing the reset operation;
s38, the processing unit compares the first turbidity with a first preset turbidity, and if the first turbidity is greater than or equal to the first preset turbidity, the liquid feeding operation, the sterilizing operation, the filtering operation, the detecting operation, the liquid discharging operation and the resetting operation are sequentially and repeatedly executed;
and S39, if the first turbidity is smaller than the first preset turbidity, sending a completion instruction to the display unit, and then starting a sterilization operation, wherein the completion instruction is an instruction for enabling the display unit to display text information prompting an operator to clean the finished product, preferably, the starting of the sterilization operation is that the operator presses the sterilization button to start the ultraviolet lamp 6 to sterilize the cleaning mechanism, and the starting of the sterilization operation is started after the operator takes out the centrifuge tube 5.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (10)

1. A tissue mass washing device, comprising:
a partition plate (2);
the clamping mechanism (3) is arranged on the lower surface of the partition plate (2), and the clamping mechanism (3) is used for clamping a centrifugal tube (5); and
the cleaning mechanism is arranged above the partition plate (2) and used for cleaning tissue blocks in the centrifugal tube (5).
2. The tissue mass washing device of claim 1, wherein: the wiper mechanism is including supplying liquid mechanism, degerming mechanism, separating mechanism and flowing back mechanism, supply liquid mechanism be used for to carry the washing liquid in centrifuging tube (5), degerming mechanism is used for making have the fungus mucus on the tissue piece break away from tissue piece, separating mechanism is used for making in centrifuging tube (5) the washing liquid with tissue piece alternate segregation, flowing back mechanism is used for discharging in centrifuging tube (5) the washing liquid.
3. The tissue mass washing device of claim 2, wherein: the liquid supply mechanism comprises a second liquid collecting barrel (12), a first water pump (14) and a liquid inlet pipe (16), the second liquid collecting barrel (12) is connected with a water inlet of the first water pump (14) through a hose, a water outlet of the first water pump (14) is connected with the liquid inlet pipe (16) through a hose, and the liquid inlet pipe (16) is arranged above a pipe orifice of the centrifugal pipe (5).
4. The tissue mass washing device of claim 2, wherein: separating mechanism is including flexible motor (13), hollow tube (110) and filter screen (19), the flexible end of flexible motor (13) with the top of hollow tube (110) is connected, the bottom downwardly extending of hollow tube (110) to in centrifuging tube (5), the bottom of hollow tube (110) with filter screen (19) fixed connection, be provided with on the lateral wall of hollow tube (110) and be used for preventing intake pipe (17) from producing slip opening (111) of maloperation, be provided with on filter screen (19) and be used for preventing intake pipe (17) produces the through-hole of maloperation.
5. The tissue mass washing device of claim 2, wherein: degerming mechanism includes air pump and intake pipe (17), the gas outlet of air pump pass through the hose with intake pipe (17) are connected, intake pipe (17) are the L type, the vertical section setting of intake pipe (17) is in hollow tube (110), just the through-hole of setting on filter screen (19) is passed to the bottom of vertical section, and downwardly extending extremely the inner chamber bottom of centrifuging tube (5), the horizontal segment of intake pipe (17) passes the setting and is in slip opening on hollow tube (110) with the top of vertical section is connected, just intake pipe (17) with the relative position of centrifuging tube (5) is fixed.
6. The tissue mass washing device of claim 2, wherein: the liquid discharge mechanism comprises a first liquid collecting barrel (11), a second water pump (15) and a liquid suction gravity ball (18), the first liquid collecting barrel (11) is connected with a water outlet of the second water pump (15) through a hose, a water inlet of the second water pump (15) is connected with the liquid suction gravity ball (18) through a hose, and the liquid suction gravity ball (18) is arranged on the upper surface of a filter screen (19) and follows the filter screen (19) to move.
7. The tissue mass washing device of claim 1, wherein: the upper surface of baffle (2) is provided with box (7), wiper mechanism sets up go up in box (7), it is provided with window (71) on the lateral wall of box (7) to go up, be provided with control module (8) on the lateral wall of last box (7), control module (8) are used for control wiper mechanism washs tissue piece in centrifuging tube (5).
8. The tissue mass washing device of claim 7, wherein: the control module (8) comprises a processing unit, a display unit, an adjusting unit and a detection unit;
the processing unit is used for receiving and processing the monitoring numerical value sent by the detection unit and sending a corresponding control instruction to the cleaning mechanism according to the monitoring numerical value, and the control instruction is used for controlling the cleaning mechanism to clean the tissue blocks in the centrifuge tube (5);
the display unit is used for displaying the air inlet intensity, the air inlet frequency, the number of cycles to be cleaned and the operation duration;
the adjusting unit comprises an emergency interruption button, a disinfection button, an adjusting button, an execution button and a pipeline cleaning button;
the detection unit comprises a second hydraulic sensor arranged at the bottom of the second liquid collecting barrel (12), a first hydraulic sensor arranged at the bottom of the first liquid collecting barrel (11), a flow sensor arranged on the first water pump (14) and an optical sensor (4) arranged below the partition plate (2).
9. A method of cleaning a tissue mass cleaning device according to any one of claims 1 to 8, comprising:
screwing a clamping knob (31) on the clamping mechanism (3) to enable two arc clamping plates (32) on the clamping mechanism (3) to move away from each other, so that a clamping hole on the clamping mechanism (3) is enlarged, and when the diameter of the clamping hole is larger than the diameter of a pipe orifice of the centrifugal pipe (5), stopping screwing the clamping knob (31);
placing the centrifuge tube (5) filled with the tissue block to be cleaned in the clamping hole, screwing the clamping knob (31), moving the two arc clamping plates (32) on the clamping mechanism (3) towards each other, further clamping the outer wall of the centrifuge tube (5), and stopping screwing the clamping knob (31) when the centrifuge tube (5) is fixed on the two arc clamping plates (32);
selecting the single washing liquid consumption corresponding to the current specification of the centrifugal tube (5) through an adjusting button, and then clicking an executing button to enable the cleaning mechanism to execute the cleaning operation until the cleaning mechanism stops running, wherein at the moment, a display unit displays cleaning completion information;
an operator twists a clamping knob (31) on the clamping mechanism (3), so that two arc clamping plates (32) on the clamping mechanism (3) loosen the centrifugal tube (5), and then take out the centrifugal tube (5).
10. The cleaning method of claim 9, wherein clicking the execute button causes the cleaning mechanism to perform a cleaning operation until the cleaning mechanism stops operating, comprises:
resetting operation: starting a telescopic motor (13), enabling the telescopic motor (13) to drive a hollow pipe (110) to move up and down, further enabling the hollow pipe (110) to drive a filter screen (19) to reach an initial preset height, and enabling the filter screen (19) to be located at the middle-upper part of the centrifugal pipe (5), wherein the initial preset height is the highest station of the filter screen (19);
liquid feeding operation: starting a first water pump (14), enabling the first water pump (14) to convey the cleaning liquid in the second liquid collecting barrel (12) to a liquid inlet pipe (16) through a hose, further enabling the cleaning liquid to enter the centrifugal pipe (5), and stopping running the first water pump (14) when a flow sensor detects that the cleaning liquid dropping into the centrifugal pipe (5) reaches the preset single-time cleaning liquid consumption;
and (3) sterilization operation: starting an air pump, leading the air pump to introduce air into an air inlet pipe (17), further leading impurities on tissue blocks in the centrifugal pipe (5) to be peeled off under the flow liquid impact formed by the cleaning liquid, and stopping running the air pump after the ventilation time reaches a first preset time;
and (3) filtering operation: starting the telescopic motor (13), enabling the telescopic motor (13) to drive the hollow pipe (110) to move downwards, further enabling the filter screen (19) to move downwards, stopping running the telescopic motor (13) when the filter screen (19) reaches a preset low position, and separating the tissue block from the cleaning solution at the moment, wherein the preset low position is the lowest station of the filter screen (19);
detection operation: starting an optical sensor (4), enabling the optical sensor (4) to detect the current turbidity of the cleaning fluid above the tissue block, uploading the current turbidity to a processing unit, and recording the current turbidity as a first turbidity;
liquid drainage operation: starting a second water pump (15), wherein the second water pump (15) pumps the cleaning liquid above the filter screen (19) into a first liquid collecting barrel (11) through a liquid suction gravity ball (18) and the hose, and simultaneously starting the optical sensor (4) to detect the turbidity of the centrifugal tube (5) above the filter screen (19), and when the turbidity is smaller than or equal to a third turbidity, stopping running the second water pump (15), wherein the third turbidity is the turbidity of the centrifugal tube (5) when the cleaning liquid is not above the filter screen (19);
executing the reset operation;
the processing unit compares the first turbidity with a first preset turbidity, and if the first turbidity is greater than or equal to the first preset turbidity, the liquid feeding operation, the sterilizing operation, the filtering operation, the detecting operation, the liquid discharging operation and the resetting operation are sequentially and repeatedly executed;
and if the first turbidity is smaller than the first preset turbidity, sending a finishing instruction to the display unit, then starting the sterilization mechanism, and sending a finishing instruction to the display unit, wherein the finishing instruction is an instruction for enabling the display unit to display text information for prompting an operator to finish cleaning.
CN202110588542.5A 2021-05-28 2021-05-28 Tissue block cleaning device and cleaning method thereof Active CN113444628B (en)

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