CN113439764A - Composition for green prevention and control of angelica sinensis mouth disease and method and application thereof - Google Patents

Composition for green prevention and control of angelica sinensis mouth disease and method and application thereof Download PDF

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CN113439764A
CN113439764A CN202110959717.9A CN202110959717A CN113439764A CN 113439764 A CN113439764 A CN 113439764A CN 202110959717 A CN202110959717 A CN 202110959717A CN 113439764 A CN113439764 A CN 113439764A
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CN113439764B (en
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米永伟
龚成文
邵武平
谢志军
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Institute Of Economic Crops And Beer Material In Gansu Academy Of Agricultural Sciences Traditional Chinese Medicine Research Institute Gansu Academy Of Agricultural Sciences
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Institute Of Economic Crops And Beer Material In Gansu Academy Of Agricultural Sciences Traditional Chinese Medicine Research Institute Gansu Academy Of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/02—Amines; Quaternary ammonium compounds
    • A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00—Agricultural use
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2107/00—Impermeabilisation
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Wood Science & Technology (AREA)
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  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Inorganic Chemistry (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Soil Sciences (AREA)
  • Materials Engineering (AREA)
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Abstract

The invention relates to application of a composition consisting of a microbial agent and an organic material in green prevention and control of angelica numb-mouthed diseases, which is characterized by comprising the microbial agent and the organic material, wherein the mass ratio of the microbial agent to the organic material is 3:1-8:1, the microbial agent is trichoderma and bacillus subtilis, and the organic material comprises polyphenol and organic matters. The composition is a green biological preparation, is safe, has no toxic or side effect, is small in application amount, quick in effect, low in cost and suitable for field irrigation or foliage spraying compared with chemical reagents. The green protection method has no damage to soil environment, and the planted angelica has high yield, good quality, safety and no toxicity, is suitable for safely taking Chinese medicinal materials, and can be mixed with most common pesticides for use.

Description

Composition for green prevention and control of angelica sinensis mouth disease and method and application thereof
Technical Field
The invention relates to the field of biology, and in particular relates to application of a composition consisting of a microbial agent and an organic material in green prevention and control of angelica numb-mouthed diseases and a method thereof.
Background
The Chinese angelica rough mouth disease mainly occurs in the Chinese angelica medicament forming period, diseased plants have no obvious symptoms on the ground, the disease mainly occurs at roots, the appearance of the roots has no symptom expression in the early infection stage, but the longitudinal section of the disease can be seen as a brown bran rot longitudinal section, after the disease develops, the epidermis of the roots is longitudinally cracked into yellow brown cracks, the depth is about 1-2mm, and the whole cortex tissue of the roots is dry and rotten in severe cases. According to experimental research, the incidence rate of the leprosy reaches more than 80 percent and the yield is only 6390kg/hm in the plots planted with the angelica in successive years2The yield of the special Chinese angelica is only about 5 percent, the yield of the first-class Chinese angelica is only about 28 percent, and the yield of the second-class, the third-class and the equal-class Chinese angelica is as high as 67.0 percent. Therefore, the Chinese angelica root tea can thoroughly and radically cure the disease of the mouth and is an important measure for solving the problems of yield reduction and quality reduction of the Chinese angelica.
The existing medicament for preventing and treating the mouth disease of the angelica sinensis mainly comprises chemical medicaments, is more in variety, has the problems of abuse, overdose, excessive pesticide residue, low prevention effect and the like, causes harm to human health in the use process, and also causes great pollution to angelica sinensis medicinal materials and the environment. Therefore, there is a need to develop safer and more effective green biologics to replace chemical pesticides.
Disclosure of Invention
The invention aims to provide application of a composition consisting of a microbial agent and organic materials in green prevention and control of the radix angelicae sinensis rough mouth disease, wherein the ratio of the microbial agent to the organic materials is 3:1-8:1, the microbial agent comprises trichoderma and bacillus subtilis, and the organic materials comprise polyphenol and organic matters.
According to the preferred technical scheme, the ratio of the microbial agent to the organic material is 25:6-25: 4.
According to the preferable technical scheme, the mass ratio of the trichoderma to the bacillus subtilis is 2:1-4: 1.
According to the preferable technical scheme, the trichoderma and the bacillus subtilis are liquid microbial inoculums.
According to the preferable technical scheme, the number of effective viable bacteria in the microbial agent is not less than 5 hundred million/g, and preferably not less than 10 hundred million/g.
According to the preferable technical scheme of the invention, the content of polyphenol in the organic material is not less than 5%, preferably not less than 10%.
According to the preferable technical scheme of the invention, the organic matter content in the organic material is not less than 10%, preferably not less than 35%.
In the preferred technical scheme of the invention, the organic material also contains N, P2O5、k2O，N、P2O5、k2The total content of O is not less than 5%, preferably not less than 8%.
According to the preferable technical scheme, the organic material also comprises an organic plant extract, the content of the organic plant extract is not less than 30%, preferably not less than 36%, and the content of polyphenol in the organic plant extract is not less than 5%, preferably not less than 10%.
In a preferred embodiment of the present invention, the organic plant extract is extracted from the plant by using a neutral organic solvent, water or steam.
According to the preferable technical scheme, the organic material is a water-soluble substance (or has water solubility), and the pH value is 7.5-9.5.
In the preferable technical scheme of the invention, the organic material is a water-soluble substance (or has water solubility), wherein the content of organic matters is not less than 10 percent, N, P2O5、k2The total content of O is not less than 6%, the content of water insoluble substance is not more than 2%, and the content of polyphenol is not less than 10%.
According to the preferable technical scheme, the organic material is a water-soluble substance (or has water solubility), the organic fertilizer is a water-soluble substance, wherein the content of organic matters is not less than 16%, the content of total nitrogen is not less than 2.5%, the content of total phosphorus is not less than 0.5%, the content of total potassium is not less than 3%, and the content of polyphenol is not less than 5%.
According to the preferable technical scheme, the organic material is any one or combination of ganle, phente and dikanghai Ann.
According to the preferable technical scheme, the composition is used in a mode that firstly, the composition is measured according to 1160-.
According to the preferable technical scheme of the invention, the foliar spraying frequency is 2-3 times, and the foliar spraying frequency is 1 time every 10-15 days.
Unless otherwise indicated, when the present invention relates to percentages between liquids, said percentages are volume/volume percentages; the invention relates to the percentage between liquid and solid, said percentage being volume/weight percentage; the invention relates to the percentages between solid and liquid, said percentages being weight/volume percentages; the balance being weight/weight percent.
Unless otherwise indicated, the present invention employs the following methods for detection:
1. the polyphenol content determination method comprises the following steps: TAHFIA 005-2018 spectrophotometry for determining total polyphenol content in plant extract and its product
2. Nitrogen, phosphorus, potassium content: NY/T1977-2010 determination of total nitrogen, phosphorus and potassium contents of water-soluble fertilizer
3. Organic matter content: NY/T1976-2010 determination of organic matter content of water-soluble fertilizer
Compared with the prior art, the invention has the following beneficial technical effects:
1. according to the invention, the microbial agent and the polyphenol substances in the organic material are utilized, on one hand, the nematode is effectively avoided, and on the other hand, the plant root system is stimulated to further grow, so that the growth and the propagation of the nematode are limited by strengthening the plant root system and directly influencing the habit of the nematode, and the effect of reducing the morbidity of the leprosy is achieved. Meanwhile, the compound can be synthesized with metal ions to take effect, the circulation from nitrogen to mineral nitrogen is influenced, the fixation of nitrogen is increased, the utilization rate of phosphorus is improved, and the loss of nutrient substances is reduced.
2. The composition can improve the soil aggregate structure and water retention capacity, promote the growth and reproduction of beneficial microorganisms, and simultaneously promote the growth of plants, photosynthesis and improvement of root system activity, thereby improving disease resistance and stress resistance.
3. The composition disclosed by the invention can be used for adjusting the pH value of soil, inhibiting soil acidification and rapid loss of nitrogen elements, solidifying heavy metals, improving soil organic matters, reducing the toxicity of soil chemical residues, reducing the conductivity and salt damage of soil and soil biodiversity, and simultaneously realizing disease resistance, yield increase and income increase.
4. The composition is a green biological preparation, is safe, has no toxic or side effect, is small in application amount, quick in effect, low in cost and suitable for field irrigation or foliage spraying compared with chemical reagents. The green protection method has no damage to soil, and the planted angelica has high yield, good quality, safety and no toxicity, is suitable for the safety of the traditional Chinese medicinal materials, and can be mixed with most of common pesticides for use.
Drawings
FIG. 1 comparison of disease rates of Angelica sinensis in examples 1-2 and control group
FIG. 2 comparison of the yields of Angelica sinensis in the practical group 1-2 and the control group
FIG. 3 comparison of the branch ratios of Angelica sinensis in the examples 1-2 and the control group
FIG. 4 crude radix Angelicae sinensis comparisons of examples 1-2 and control
FIG. 5 Angelica root-weight comparison of examples 1-2 and control
FIG. 6 comparison of the main root lengths of Dang Gui in the examples 1-2 and the control group
Detailed Description
EXAMPLE 1 preparation of a composition according to the invention
The composition comprises a microbial agent and an organic material, wherein the mass ratio of the microbial agent to the organic material is 25: 4.
The microbial agent is obtained by compounding a liquid microbial agent trichoderma and a liquid microbial agent bacillus subtilis according to the mass ratio of 2:1, wherein the effective viable count of the liquid microbial agent trichoderma is more than or equal to 5 hundred million/g, and the effective viable count of the liquid microbial agent bacillus subtilis is more than or equal to 5 hundred million/g.
The organic material was Ganle, purchased from Suzhou Fumei plant protectant, Inc. The polyphenol content in Ganle is 6.5%, the organic matter content is greater than or equal to 10%, N, P2O5、k2The total content of O is more than or equal to 6 percent.
Example 2 preparation of a composition according to the invention
The composition comprises a microbial agent and an organic material, wherein the mass ratio of the microbial agent to the organic material is 25: 6.
The microbial agent is obtained by compounding a liquid microbial agent trichoderma and a liquid microbial agent bacillus subtilis according to the mass ratio of 2:1, wherein the effective viable count of the liquid microbial agent trichoderma is more than or equal to 5 hundred million/g, and the effective viable count of the liquid microbial agent bacillus subtilis is more than or equal to 5 hundred million/g.
The organic material is phente, and is purchased from chemical engineering construction limited of China. The content of organic wood extract in the phentermine is 36% (wherein the content of polyphenol is 30%), and the content of organic carbon is 20%.
Test examples
1 general description of test region
The test is carried out in Wunancun Chuanxiang, Gansu Ming county, and the soil type is Heikou soil. The altitude of a test area is 2560m, the climate is high, cold and damp, the temperature difference between day and night is large, the average annual temperature is 4.8 ℃, the frost-free period is about 105d, the average annual precipitation is about 589.5mm, and the rain season is mainly concentrated in 5-9 months and accounts for 82.6% of the annual precipitation.
2 design of the experiment
The first stubble of the experimental plot is Dang Gui. Experimental design the experimental design was performed in groups 1-2 and the control group for a total of 3 treatments (see table 1 for details), which were repeated 3 times. The test totals 9 cells, the area is 30.0m2The method adopts a film side planting method, the film width is 35cm, the row spacing is 40cm, the plant spacing is 15cm, the planting density is 11112 plants/mu, and the seedling number is 7800 plants/mu. Gently cutting off aerial parts of radix Angelicae sinensis in autumn after the aerial parts are withered and yellow, and randomly selecting 3m for each cell2Taking 3 points (1m multiplied by 3m) as sample prescriptions, digging out Chinese angelica plants in each sample prescription by using a three-tooth steel fork, recording the total plant number of medicinal materials in a plot, removing impurity and residual stems, weighing, randomly extracting 20 complete Chinese angelica medicinal materials, respectively measuring indexes such as root length, main root length, root thickness, fresh weight of single root, branch number and the like, and counting the disease condition.
TABLE 1
Figure BDA0003221779040000071
4 results and analysis
The disease statistics show that the compositions of examples 1-2 all had a certain degree of control effect on the numb mouth disease of angelica, compared to the control group (fig. 1).
The angelica yield results show that the compositions of examples 1-2 all have a certain degree of promotion effect on the angelica yield compared with the control group (fig. 2).
The quality characteristics of the angelica sinensis medicinal materials show that compared with the control group, the compositions in the examples 1-2 have certain improvement effects on the quality characteristics of the medicinal materials such as main root length, branch number, rhizome thickness and single root weight of the angelica sinensis (figures 3-6).
The above description of the specific embodiments of the present invention is not intended to limit the present invention, and those skilled in the art may make various changes and modifications according to the present invention without departing from the spirit of the present invention, which is defined in the appended claims.

Claims (10)

1. The application of a composition consisting of a microbial agent and organic materials in preventing and treating the numb mouth disease of angelica is characterized in that the ratio of the microbial agent to the organic materials is 3:1-8:1, the microbial agent comprises trichoderma and bacillus subtilis, and the organic materials comprise polyphenol and organic matters.
2. The use as claimed in claim 1, wherein the ratio of microbial agent to organic material is from 25:6 to 25: 4.
3. The use according to any one of claims 1 to 2, wherein the mass ratio of the trichoderma to the bacillus subtilis is 2:1-4:1, the trichoderma and the bacillus subtilis are preferably liquid microbial agents, and the number of effective viable bacteria in the microbial agents is not less than 5 hundred million/g, and is preferably not less than 10 hundred million/g.
4. Use according to any one of claims 1 to 3, wherein the organic material has a polyphenol content of not less than 5%, preferably not less than 10%, and the organic material has an organic content of not less than 10%, preferably not less than 35%, and the organic material further comprises N, P2O5、k2O，N、P2O5、k2The total content of O is not less than 5%, preferably not less than 8%.
5. The use as claimed in any one of claims 1 to 4, wherein the organic material further comprises an organic plant extract, the organic plant extract being present in an amount of not less than 30%, preferably not less than 36%, and wherein the organic plant extract contains not less than 5%, preferably not less than 10% polyphenols.
6. The use according to any one of claims 1 to 5, wherein the organic plant extract is extracted from the plant using a neutral organic solvent, water or steam.
7. The use according to any one of claims 1 to 6, wherein the organic material is a water-soluble material having a pH of 7.5 to 9.5.
8. The use according to any one of claims 1 to 7, wherein the organic material is a water soluble material having an organic content of not less than 10%, N, P2O5、k2The total content of O is not less than 6%, the content of water insoluble substance is not more than 2%, and the content of polyphenol is not less than 10%.
9. The use according to any one of claims 1 to 8, wherein the organic material is a water soluble material having an organic content of not less than 16%, a total nitrogen content of not less than 2.5%, a total phosphorus content of not less than 0.5%, a total potassium content of not less than 3%, and a polyphenol content of not less than 5%.
10. The use as claimed in any one of claims 1 to 9, wherein the composition is used by firstly measuring the composition at 1160-1240 ml/mu, diluting 500 times, then performing root drenching treatment during transplanting angelica seedlings, secondly measuring the composition at 580-620 ml/mu after transplanting angelica seedlings for 40-60 days, diluting 500 times, then performing foliar spraying of angelica, preferably the foliar spraying frequency is 2-3 times, and spraying 1 time every 10-15 days.
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