CN113424830B - Application of bactericidal composition containing fluazinam and epoxiconazole in preventing and treating bakanae disease and false smut of rice - Google Patents
Application of bactericidal composition containing fluazinam and epoxiconazole in preventing and treating bakanae disease and false smut of rice Download PDFInfo
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- CN113424830B CN113424830B CN202110764207.6A CN202110764207A CN113424830B CN 113424830 B CN113424830 B CN 113424830B CN 202110764207 A CN202110764207 A CN 202110764207A CN 113424830 B CN113424830 B CN 113424830B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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Abstract
The invention provides an application of a sterilization composition containing fluazinam and epoxiconazole in preventing and treating rice bakanae disease, wherein the sterilization composition contains the fluazinam and the epoxiconazole with the weight ratio of 1:8-16. The invention also provides application of the bactericidal composition containing fluazinam and epoxiconazole in preventing and treating false smut of rice, wherein the bactericidal composition contains the fluazinam and the epoxiconazole with the weight ratio of 1. The bactericidal composition has obvious synergy effect through the toxicity test verification on the bakanae disease of rice and the false smut disease of rice. The composition can exert activity at each stage of disease infection, the using time is more loose, the using times and labor cost are reduced, the defect that a single-dose bactericidal compound is easy to form resistance in the using process is overcome, and the obtained bactericidal composition has the advantages of high efficiency, broad spectrum, durability and the like, and has remarkable control effects on rice bakanae disease, false smut, rice blast and rice sheath blight.
Description
[ technical field ] A method for producing a semiconductor device
The bactericide composition of the present invention has fluazinam and epoxiconazole as effective components and is suitable for preventing rice bakanae disease and false smut. Belongs to the new application of bactericide in the field of pesticide.
[ background of the invention ]
Fluazinam is a dinitroaniline protective bactericide developed by Nippon stone companies, has the advantages of wide bactericidal spectrum, long lasting period, rain wash resistance and low systemic property, and cannot permeate into leaves or roots. Fluazinam was approved in the united states as a low environmental risk product in 2001. The preparation is a mitochondrial oxidative phosphorylation uncoupler for inhibiting the respiration of pathogenic bacteria, can inhibit the infection process of all stages of the pathogenic bacteria such as spore germination, hypha breakthrough, growth, spore formation and the like, and is very effective for preventing and treating diseases caused by sclerotinia, sclerotinia and cladosporium. It is also effective against Botrytis cinerea which is a fungicide of benzimidazoles and dicarboximides. In addition to bactericidal activity, fluazinam also exhibits acaricidal activity against red spiders and the like. Has excellent control effect on clubroot of cruciferae plants and also has good control effect on rice damping-off caused by rhizopus.
Epoxiconazole is a systemic triazole fungicide, and can inhibit synthesis of ergosterol of pathogenic bacteria, inhibit formation of cell walls of pathogenic bacteria, and inhibit invasion of pathogenic bacteria. The epoxiconazole can effectively control diseases, and can improve the self biochemical disease resistance of crops by regulating the activity of enzyme, so that the disease resistance of the crops is greatly enhanced. It has excellent preventing and treating effect on crop diseases, such as damping off, powdery mildew, eyespot, etc. and can prevent and treat sugar beet, peanut, rape, lawn, coffee, rice, fruit tree, etc. It has not only good protective, therapeutic and eradicating activity, but also systemic and better residual activity.
The rice bakanae disease is a fungal seed-borne disease in the global scope, the main pathogenic bacteria are fusarium which mainly damage the overground part of rice, and the occurrence range is mainly distributed in tropical and temperate rice production areas including Asian Africa and North America. In recent years, due to the introduction of high-yield varieties and the increasing planting area, and the wider application of dry-breeding film technology, the incidence rate of rice bakanae disease becomes higher and higher, and the rice bakanae disease becomes an important influence factor which harms rice production. In Asian regions, the yield loss of rice caused by bakanae disease can reach 70 percent at most. The bakanae disease of rice can not only reduce the setting rate of seeds, cause loss to the yield of rice and reduce the quality of rice, but also can generate a plurality of metabolites such as fusaric acid, gibberellin and fumonisin during the disease attack period, thereby causing great harm to the health of human bodies.
False smut is a common fungal disease of rice, which occurs on the ear of rice and is known as rice grain, commonly called powdery mildew, black ball disease or false smut, and the main pathogenic bacterium is Rhizoctonia solani. The rice grain infected with false smut is dark green or black, and yellow green spores are scattered. The rice has the defects of increased rice grain-shrinking rate, reduced thousand-grain weight and affected appearance and yield. In recent years, the rice field has a tendency of outbreak in some rice areas of China, and spreading is aggravated. More seriously, the ustilaginoidea virens can also generate the sclerotium virens and the ustilagin which are harmful to people and livestock, and the ustilagin directly harms the health of people.
Chinese invention patent application CN 102119690A discloses a bactericidal composition of fluazinam and epoxiconazole and application thereof, and discloses that the composition can be used for preventing and treating rice blast and sheath blight. However, the rice blast is a rice disease caused by fungi of deuteromycotina such as botrytis cinerea or pyricularia oryzae, pathogenic bacteria of sheath blight are mainly rhizoctonia solani, the pathogenic bacteria and fusarium or rhizoctonia solani belong to different pathogenic bacteria, the pathogenic mechanisms are different, and no technology for applying the composition to the rice blast or the sheath blight exists at present.
The prevention and treatment of the bakanae disease and the false smut of the rice are very important in agriculture, however, the cost for developing a new medicament is high, and the time consumption is long, so that how to obtain the high-efficiency treatment of the bakanae disease and the false smut of the rice through medicament compounding is particularly important.
[ summary of the invention ]
The invention aims to overcome the defects of the prior art, and provides a sterilization composition with control effect on bakanae disease and false smut of rice, which has the characteristics of prolonging the application period, reducing the dosage, reducing the phytotoxicity, reducing the residue and delaying the drug resistance and drug resistance of fungi.
In order to achieve the above purpose, the present invention provides an application of a bactericidal composition containing fluazinam and epoxiconazole in preventing and treating rice bakanae disease, wherein the bactericidal composition contains the fluazinam and the epoxiconazole in a weight ratio of 1:8-16.
According to a preferred embodiment, the weight ratio of fluazinam to epoxiconazole is 1:8, 2:1 to 4:1 or 16.
Based on the above, the invention also provides an agricultural bactericide for preventing and treating rice bakanae disease, which contains the bactericidal composition containing fluazinam and epoxiconazole.
Preferably, in the present invention, the agricultural fungicide further comprises a carrier and an adjuvant acceptable in agricultural chemicals.
In the present invention, the agricultural fungicide is in the form of a suspension.
On the other hand, the invention also provides application of a sterilization composition containing fluazinam and epoxiconazole in preventing and treating rice false smut, wherein the sterilization composition contains the fluazinam and the epoxiconazole in a weight ratio of 1.
According to another preferred embodiment, the weight ratio of fluazinam to epoxiconazole is 4:1.
The invention also provides an agricultural bactericide for preventing and treating rice false smut, which contains the bactericidal composition containing fluazinam and epoxiconazole.
Preferably, the agricultural fungicide further comprises a carrier and an adjuvant acceptable in pesticides.
In the present invention, the agricultural fungicide is in the form of a suspension.
For the suspending agents, usable are: the dispersant is selected from polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate, TERSPERSE 2425 (alkylnaphthalene sulfonate, available from henckie, usa); the emulsifier is selected from Nongru 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), nongru 2201, span-60 # (common name: sorbitan monostearate), emulsifier T-60 (common name: sorbitan monostearate polyoxyethylene ether), nongru 1601# (common name: phenethylphenol polyoxyethylene polyoxypropylene ether), TERSPERSE 4894 (product of Hensmei company in USA), FS3000 (phosphate anionic surfactant); the wetting agent is selected from alkylphenol polyoxyethylene polyether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, naphthalene sulfonate, TERSPERSE (made by Hensman, USA) and SXC (sulfonic acid type anionic surfactant); the thickener is selected from xanthan gum, polyvinyl alcohol, magnesium aluminum silicate, and bentonite; the preservative is selected from benzoic acid, sodium benzoate, BIT (1,2-benzisothiazolin-3-one); the antifreeze is selected from ethylene glycol, propylene glycol, glycerol, and urea.
The bactericidal composition is verified by a toxicity test on the rice bakanae disease, when the mass ratio of the fluazinam to the epoxiconazole is 1-8. On the other hand, the pesticide composition is verified by a toxicity test on false smut, when the mass ratio of the fluazinam to the epoxiconazole is 1-16.
In terms of the application period, the optimal application time of fluazinam is before or at the initial stage of disease occurrence, but the fluazinam should be used with caution in the seedling stage of crops, otherwise the growth of crops can be affected, and high temperature or strong light should be avoided when the fluazinam is applied. And the epoxiconazole has special effects of preventing and treating rice sheath blight, rice blast, false smut, sheath rot, smut, various middle and later period syndromes and the like when being applied in the breaking and heading period and after flowering. The composition can exert activity in each stage of disease infection, has more loose use time, reduces the application times and labor cost, can effectively control diseases, and can improve the self biochemical disease resistance of crops by regulating the activity of enzyme so as to greatly enhance the disease resistance of the crops.
The fluazinam and the epoxiconazole are compounded, the defect that a single-dose bactericidal compound is easy to form resistance in the using process is overcome, and the obtained bactericidal composition has the advantages of high efficiency, broad spectrum, durability and the like, and has remarkable control effects on rice bakanae disease, false smut, rice blast and rice sheath blight.
[ detailed description ] embodiments
The following examples serve to explain, without limitation, where the techniques of the present invention are put.
In the present invention, unless otherwise specified, the mentioned proportions are mass ratios and% are mass percentages.
Example 1 indoor bioassay test
After the effective inhibition concentration range of each medicament is determined by pre-test for indoor toxicity measurement of fluazinam and epoxiconazole on rice bakanae disease and ustilaginoidea virens in different proportions, each medicament is respectively treated with 5 doses, and the treatment of a solvent is used as a reference. The method is carried out according to 'indoor pesticide bioassay test standard bactericide', cultured rice bakanae bacteria and ustilaginoidea virens strains are respectively cultured for 7d and 28d at 25 ℃ by using a sterilization puncher with the diameter of 0.5cm under aseptic conditions to punch bacterium dishes at the edges of bacterial colonies, the hypha surfaces face upwards, the diameters of the bacterial colonies are measured by adopting a cross method, and the inhibition rate of the medicament on the growth of the hypha is calculated by comparing with a control. The Synergistic Ratio (SR) of different compounding ratios of the medicaments is determined, wherein SR <0.5 is antagonistic action, SR <0.5 and less than or equal to 1.5 are additive action, and SR >1.5 is synergistic action, and then field experiments are carried out on the basis.
1. Fluazinam and epoxiconazole compounded indoor toxicity determination method for rice bakanae disease
Test subjects: bacterial bakanae of rice
And (3) experimental design: the effective inhibition concentration ranges of the fluazinam and epoxiconazole technical products and the mixture of the fluazinam and the epoxiconazole technical products with different proportions are determined through preliminary experiments.
The virulence assay results are shown in table 1.
TABLE 1 indoor toxicity test results of fluazinam and epoxiconazole with different ratios for rice bakanae disease
As can be seen from table 1, the compound ratio of fluazinam to epoxiconazole is in the range of 1. Further performing field pesticide effect test under the compounding condition to evaluate the field practical application effect of the pesticide composition on the rice bakanae disease.
2. Fluazinam and epoxiconazole compounded indoor toxicity determination method for rice false smut germs
Test subjects: ustilaginoidea virens (Fr.) Kuntze
And (3) experimental design: the effective inhibition concentration ranges of the fluazinam and epoxiconazole technical products and the mixture of the fluazinam and the epoxiconazole technical products with different proportions are determined through preliminary experiments.
The virulence assay results are shown in table 2.
TABLE 2 indoor toxicity test results of fluazinam and epoxiconazole with different ratios for ustilaginoidea virens
As can be seen from Table 2, the compound ratio of fluazinam to epoxiconazole is 1-16, and the additive effect is shown in the range of 1. Further performing field efficacy test under the compounding condition to evaluate the field practical application effect of the compound on rice false smut.
Example 2 preparation of a 9% concentration of a fluazinam and epoxiconazole suspension seed coating agent (ratio of active ingredients 1:8)
Weighing 1% fluazinam technical, 8% epoxiconazole technical, 5% farm milk 1601, 2% bentonite, 0.3% xanthan gum, 5% ethylene glycol and 0.2% polyvinyl alcohol blending modification film forming agent, and adding deionized water to 100%. According to the formula proportion, water is used as a medium, the active ingredients, the bentonite, the emulsifier and part of water are fully mixed, stirred and then sanded, and then the film-forming agent, the thickening agent, the antifreezing agent and the balance of water are sequentially added into a preparation kettle and fully mixed to obtain the suspended seed coating preparation product.
Example 3 preparation of a suspension of fluazinam and epoxiconazole having a concentration of 50% (effective ingredient ratio 4:1)
Weighing 40% of fluazinam technical, 10% of epoxiconazole technical, 6% of phosphate ester emulsifier, 0.2% of polysiloxane defoamer, 0.3% of xanthan gum and deionized water to 100%. According to the formula proportion, water is used as a medium, the metered components are added into a high-shear emulsifying machine for shearing for 30min, and then a sand mill is used for sanding to obtain the suspending agent.
Example 4 field efficacy test
1. Efficacy test of fluazinam, epoxiconazole and compound agent thereof for preventing and treating rice bakanae disease
And (3) test treatment: the test medicament is prepared from 50% of fluazinam suspending agent, 12.5% of epoxiconazole suspending agent and the seed coating agent prepared in example 2, and the dosage of the effective components is set as the dosage of the test medicament, and clear water is set for comparison.
The test mode is as follows: the above-mentioned chemicals are respectively used for soaking rice seeds for 48 hr, and its temperature is 25 deg.C, then the germination can be accelerated to make rice seeds appear white. The seedling raising mode is dry seedling raising, and the seedling is transplanted to a field after the seedling age is five leaves. And investigating the plant disease rate and calculating the control effect in the seedling stage and the filling stage.
The test results are shown in table 3:
TABLE 3 field test of pesticide effect of seed soaking treatment of fluazinam and epoxiconazole for preventing and treating bakanae disease of rice
As can be seen from Table 3, the control effect of the fluazinam and the epoxiconazole is higher when the compound ratio is 1:8 than when each medicament is used independently. Therefore, the compound pesticide has better prevention effect on the bakanae disease of rice, can improve pesticide effect, reduce cost and prolong the service life of the pesticide, and has excellent economic value.
2. Drug effect test of fluazinam, epoxiconazole and compound agent thereof for preventing and treating rice false smut
And (3) test treatment: the test medicament dosage is the dosage of the effective components, the treatment is respectively 50 percent of fluazinam suspending agent, 12.5 percent of epoxiconazole suspending agent and the suspending agent in the embodiment 3, and clear water is set for comparison.
The test mode is as follows: each cell area is 100m 2 The pesticide is sprayed on the whole plant at the early stage of rice spike pregnancy until the pesticide amount is dripped on the rice leaf surface and the rice straw. The ear disease rate, disease index and prevention and treatment effect are investigated after 21 days.
Grading standard of rice false smut disease: grade 0 is no disease; grade 1 is 1 rice koji per ear; grade 2 is 2 rice koji per ear; grade 3 is 3-5 rice koji per ear; grade 4 is 6-9 rice koji per ear; grade 5 is more than 10 rice koji per ear. The calculation formula is as follows:
the test results are shown in Table 4:
TABLE 4 field test of the efficacy of fluazinam and epoxiconazole on false smut of rice
As shown in Table 4, when the compound ratio of fluazinam and epoxiconazole is 4:1, the control effect on rice false smut is higher than that when each medicament is used independently. Therefore, the compound medicament has better control effect on the false smut of rice, can improve the pesticide effect, reduce the cost and delay the resistance, and has important production significance.
In conclusion, the fluazinam and the epoxiconazole are compounded, the defect that a single-agent bactericidal compound is easy to form resistance in the using process is overcome, and the obtained bactericidal composition has the advantages of high efficiency, broad spectrum, durability and the like, and has obvious control effects on rice bakanae disease, false smut, rice blast and rice sheath blight.
Claims (2)
1. The application of the sterilization composition containing fluazinam and epoxiconazole in preventing and treating the rice bakanae disease comprises 1:8, 2:1, 4:1 or 16 in weight ratio.
2. The application of the bactericidal composition containing fluazinam and epoxiconazole in preventing and treating rice false smut comprises 4:1 of fluazinam and epoxiconazole in weight ratio.
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| CN202110764207.6A CN113424830B (en) | 2021-07-06 | 2021-07-06 | Application of bactericidal composition containing fluazinam and epoxiconazole in preventing and treating bakanae disease and false smut of rice |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102119690B (en) * | 2010-01-08 | 2013-01-23 | 南京华洲药业有限公司 | Bactericidal composition containing fluazinam and epoxiconazole and application of same |
| CN101984822B (en) * | 2010-08-06 | 2012-12-12 | 东莞市瑞德丰生物科技有限公司 | Germicide composition |
| CN102273442A (en) * | 2011-09-13 | 2011-12-14 | 广西田园生化股份有限公司 | Ultralow volume liquid containing epoxiconazole |
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