CN113412890A - Preparation method of grape caulerpa extract and application of grape caulerpa extract in formula feed for ornamental fish - Google Patents
Preparation method of grape caulerpa extract and application of grape caulerpa extract in formula feed for ornamental fish Download PDFInfo
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Abstract
The invention discloses a preparation method of a grape fern algae extract and application of the grape fern algae extract in formula feed for ornamental fishes, which comprises the following steps: step one, cleaning and soaking; step two, drying at high temperature; step three, crushing and sieving; step four, primary extraction; step five, centrifugal layering; step six, separating and storing; step seven, secondary extraction; step eight, weighing raw materials; step nine, mixing and homogenizing; step ten, spray drying; step eleven, puffing and granulating; step twelve, packaging and warehousing, the method increases the permeation between the solvent and the extract through cavitation, shearing and heat effects of ultrasonic waves, is favorable for improving the extraction efficiency, performs secondary extraction on the extracted residues, is favorable for realizing thorough extraction, reduces resource waste, prepares the fish feed by taking the caulerpa longipedicellata extract as a formula, is favorable for improving the immune function of fish, improves the survival rate, effectively prevents the oxidation of fat in the feed, and promotes the healing of fish wounds.
Description
Technical Field
The invention relates to the technical field of feed production, in particular to a preparation method of a grape caulerpa extract and application of the grape caulerpa extract in formula feed for aquarium fishes.
Background
Caulerpa lentillifera belongs to Caulerpa of Chlorophyta, is mainly distributed in Japan, Indonesia, Vietnam and the like, and is introduced into China for cultivation in large quantities in recent years. Its appearance is glittering and translucent and plump like grape, so that it is called sea grape, it is an edible green algae with rich nutrients, it contains several nutrient substances, and has the functions of resisting cancer, resisting tumor, resisting oxidation, raising immunity, activating brain cell, purifying blood and beautifying face, 80% of long-stem grape fern algae is formed from algal polysaccharide, collagen and dietary fibre, and its in vivo detected 16 amino acids are 11 amino acids necessary for human body, and 7 amino acids are important components of protein.
The nutritional value of the caulerpa lentillifera is extremely high, especially the protein content is considerable, how to extract the nutrient substances in the caulerpa lentillifera is always the key point of research, the existing preparation method of the caulerpa lentillifera extract wastes time and labor in the extraction process, the working efficiency is reduced, various reagents are adopted for extraction, the cost is high, the extraction is not thorough, the resource waste is easily caused, the application range of the caulerpa lentillifera extract is very wide, the application is also realized in the production aspect of ornamental fish feed, the existing ornamental fish feed is easy to scatter and swell when entering water, the nutritional value is low, and the quality of the fish feed is reduced.
Disclosure of Invention
The invention aims to provide a preparation method of a grape fern algae extract and application of the grape fern algae extract in formula feed for ornamental fishes, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the preparation method of the grape fern extract and the application of the grape fern extract in the formula feed for the aquarium fishes comprise the following steps: step one, cleaning and soaking; step two, drying at high temperature; step three, crushing and sieving; step four, primary extraction; step five, centrifugal layering; step six, separating and storing; step seven, secondary extraction; step eight, weighing raw materials; step nine, mixing and homogenizing; step ten, spray drying; step eleven, puffing and granulating; step twelve, packaging and warehousing;
firstly, taking a proper amount of caulerpa lentillifera, then cleaning dust and impurities on the surface of the caulerpa lentillifera by using distilled water, then soaking the caulerpa lentillifera by using the distilled water, taking out the caulerpa lentillifera after soaking, and sucking water by using filter paper for later use;
in the second step, the caulerpa lentillifera which is dried in the first step is put into a drying box for drying, is taken out after being dried to constant weight, and is cooled to room temperature for later use;
in the third step, the dried caulerpa lentillifera obtained in the second step is put into a grinder to be ground, and the ground caulerpa lentillifera is sieved by a 60-mesh sieve, so that uniform and fine caulerpa lentillifera powder is left for later use;
in the fourth step, the caulerpa lentillifera powder prepared in the third step is put into a glass flask, then the extracting solution is poured into the glass flask, and then the glass flask is subjected to ultrasonic-assisted extraction by utilizing ultrasonic waves;
in the fifth step, after the extraction in the fourth step is finished, putting the glass flask into a centrifuge for centrifugal layering, separating the extract and impurities, centrifuging and standing for later use;
in the sixth step, a dropper is used for sucking out the supernatant in the glass flask after standing and layering in the fifth step, and then the supernatant is put into a glass bottle after cleaning and disinfection for standby;
in the seventh step, taking a proper amount of extracting solution, pouring the extracting solution into the glass flask after the supernatant is taken out in the sixth step again, then repeating the processes in the fourth step and the fifth step, carrying out secondary extraction, carrying out centrifugal layering, sucking the supernatant by using a dropper again after the secondary extraction and the centrifugal layering are finished, and adding the supernatant into the glass flask for later use, thereby finishing the preparation of the grape fern algae extract;
in the eighth step, respectively weighing 40-50 parts by weight of carbohydrate, 10-15 parts by weight of bean cake powder, 0.5-1 part by weight of stabilizer, 0.1-0.5 part by weight of emulsifier, 0.3-0.5 part by weight of vitamin powder, 1-1.5 parts by weight of bone meal and 0.05-0.1 part by weight of edible pigment, and then weighing 15-20 parts by weight of the fiddlehead extract prepared in the seventh step;
sequentially putting the carbohydrate, the bean cake powder, the stabilizer, the emulsifier, the vitamin powder, the bone meal, the edible pigment and the caulerpa vinifera extract weighed in the step eight into a high-pressure homogenizer for mixing and homogenizing, and preparing into homogenate for later use after mixing;
in the above step ten, the homogenate prepared in the step nine is put into a spray dryer for spray drying, and raw material particles are prepared for standby after drying;
in the eleventh step, the raw material particles after the spray drying in the tenth step are put into a single-screw extrusion puffing machine for extrusion puffing, and then the raw material particles are added into a granulator for granulation to prepare fish feed particles;
and a twelfth step of vacuum-packaging the fish feed particles prepared in the eleventh step by using a vacuum packaging machine, and then delivering the fish feed particles into a warehouse for storage.
Preferably, in the step one, the soaking time is 5-10 min.
Preferably, in the second step, the temperature of the drying oven is 105-.
Preferably, in the third step, the rotation speed of the pulverizer is 1500-.
Preferably, in the fourth step, the extracting solution is water or ethanol, and the liquid-to-material ratio of the extracting solution to the caulerpa lentillifera powder is 30 mL/g.
Preferably, in the fourth step, the ultrasonic power is 50-55W, the ultrasonic temperature is 60-65 ℃, and the ultrasonic time is 11-13 min.
Preferably, in the step eight, the carbohydrate is one or a mixture of corn flour, barley flour and sweet potato flour.
Preferably, in the ninth step, the working pressure of the high-pressure homogenizer is 30-40MPa, the working temperature is 65-70 ℃, and the working time is 30-40 min.
Preferably, in the step ten, the temperature of the spray dryer is 160-170 ℃.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention increases the penetration between the solvent and the extract by the action force of cavitation, shearing, thermal effect and the like of ultrasonic waves, is beneficial to improving the extraction efficiency and shortening the extraction time, and adopts a physical mode to assist the extraction, thereby reducing the dosage of chemical reagents and reducing the extraction cost;
2. according to the invention, the extracted residues are subjected to secondary extraction, so that the extraction degree is improved, thorough extraction is facilitated, resource waste is reduced, and the extraction yield is increased;
3. the invention takes the caulerpa longipedicellata extract as the raw material to prepare the fish feed, which is beneficial to improving the immunologic function of fish, improving the survival rate, effectively preventing the oxidation of fat in the feed, promoting the wound healing of the fish, having the detoxification function and enhancing the resistance function of the fish to the adverse water environment.
Drawings
FIG. 1 is a flow chart of the method of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, an embodiment of the present invention:
example 1:
the preparation method of the grape fern extract and the application of the grape fern extract in the formula feed for the aquarium fishes comprise the following steps: step one, cleaning and soaking; step two, drying at high temperature; step three, crushing and sieving; step four, primary extraction; step five, centrifugal layering; step six, separating and storing; step seven, secondary extraction; step eight, weighing raw materials; step nine, mixing and homogenizing; step ten, spray drying; step eleven, puffing and granulating; step twelve, packaging and warehousing;
firstly, taking a proper amount of caulerpa lentillifera, then cleaning dust and impurities on the surface of the caulerpa lentillifera by using distilled water, then soaking the caulerpa lentillifera by using the distilled water for 10min, taking out the caulerpa lentillifera after soaking, and sucking water by using filter paper for later use;
in the second step, the caulerpa lentillifera which is dried in the first step is put into a drying oven for drying until the weight is constant, the caulerpa lentillifera is taken out, the temperature of the drying oven is 105 ℃, the drying time is 2.5 hours, and the caulerpa lentillifera is cooled to room temperature for later use;
in the third step, the dried caulerpa lentillifera obtained in the second step is put into a pulverizer to be pulverized, and the pulverized caulerpa lentillifera is sieved by a 60-mesh sieve, wherein the rotation speed of the pulverizer is 2000r/min, the pulverizing time is 8min, and uniform and fine caulerpa lentillifera powder is left for later use;
in the fourth step, the caulerpa lentillifera powder prepared in the third step is put into a glass flask, then the extracting solution is poured into the glass flask, the extracting solution is water or ethanol, the liquid-material ratio of the extracting solution to the caulerpa lentillifera powder is 30mL/g, and then the glass flask is subjected to ultrasonic-assisted extraction by utilizing ultrasonic waves, wherein the ultrasonic power is 50W, the ultrasonic temperature is 60 ℃, and the ultrasonic time is 11 min;
in the fifth step, after the extraction in the fourth step is finished, putting the glass flask into a centrifuge for centrifugal layering, separating the extract and impurities, centrifuging and standing for later use;
in the sixth step, a dropper is used for sucking out the supernatant in the glass flask after standing and layering in the fifth step, and then the supernatant is put into a glass bottle after cleaning and disinfection for standby;
in the seventh step, taking a proper amount of extracting solution, pouring the extracting solution into the glass flask after the supernatant is taken out in the sixth step again, then repeating the processes in the fourth step and the fifth step, carrying out secondary extraction, carrying out centrifugal layering, sucking the supernatant by using a dropper again after the secondary extraction and the centrifugal layering are finished, and adding the supernatant into the glass flask for later use, thereby finishing the preparation of the grape fern algae extract;
in the eighth step, respectively weighing 40 parts of carbohydrate, 10 parts of bean cake powder, 0.5 part of stabilizer, 0.1 part of emulsifier, 0.3 part of vitamin powder, 1 part of bone meal and 0.05 part of edible pigment according to parts by weight, and then weighing 15 parts of the fiddlehead fern algae extract prepared in the seventh step, wherein the carbohydrate is one or a mixture of corn flour, barley flour and sweet potato flour;
sequentially putting the carbohydrate, the bean cake powder, the stabilizer, the emulsifier, the vitamin powder, the bone meal, the edible pigment and the grape fern algae extract weighed in the step eight into a high-pressure homogenizer for mixing and homogenizing, wherein the working pressure of the high-pressure homogenizer is 40MPa, the working temperature is 65 ℃, the working time is 30min, and the homogenate is prepared for later use after the mixing is finished;
in the tenth step, the homogenate prepared in the ninth step is put into a spray dryer for spray drying, the temperature of the spray dryer is 165 ℃, and raw material particles are prepared for standby after drying;
in the eleventh step, the raw material particles after the spray drying in the tenth step are put into a single-screw extrusion puffing machine for extrusion puffing, and then the raw material particles are added into a granulator for granulation to prepare fish feed particles;
and a twelfth step of vacuum-packaging the fish feed particles prepared in the eleventh step by using a vacuum packaging machine, and then delivering the fish feed particles into a warehouse for storage.
Example 2:
the preparation method of the grape fern extract and the application of the grape fern extract in the formula feed for the aquarium fishes comprise the following steps: step one, cleaning and soaking; step two, drying at high temperature; step three, crushing and sieving; step four, primary extraction; step five, centrifugal layering; step six, separating and storing; step seven, secondary extraction; step eight, weighing raw materials; step nine, mixing and homogenizing; step ten, spray drying; step eleven, puffing and granulating; step twelve, packaging and warehousing;
firstly, taking a proper amount of caulerpa lentillifera, then cleaning dust and impurities on the surface of the caulerpa lentillifera by using distilled water, then soaking the caulerpa lentillifera by using the distilled water for 10min, taking out the caulerpa lentillifera after soaking, and sucking water by using filter paper for later use;
in the second step, the caulerpa lentillifera which is dried in the first step is put into a drying oven for drying until the weight is constant, the caulerpa lentillifera is taken out, the temperature of the drying oven is 105 ℃, the drying time is 2.5 hours, and the caulerpa lentillifera is cooled to room temperature for later use;
in the third step, the dried caulerpa lentillifera obtained in the second step is put into a pulverizer to be pulverized, and the pulverized caulerpa lentillifera is sieved by a 60-mesh sieve, wherein the rotation speed of the pulverizer is 2000r/min, the pulverizing time is 8min, and uniform and fine caulerpa lentillifera powder is left for later use;
in the fourth step, the caulerpa lentillifera powder prepared in the third step is put into a glass flask, then the extracting solution is poured into the glass flask, the extracting solution is water or ethanol, the liquid-material ratio of the extracting solution to the caulerpa lentillifera powder is 30mL/g, and then the glass flask is subjected to ultrasonic-assisted extraction by utilizing ultrasonic waves, wherein the ultrasonic power is 50W, the ultrasonic temperature is 60 ℃, and the ultrasonic time is 11 min;
in the fifth step, after the extraction in the fourth step is finished, putting the glass flask into a centrifuge for centrifugal layering, separating the extract and impurities, centrifuging and standing for later use;
in the sixth step, a dropper is used for sucking out the supernatant in the glass flask after standing and layering in the fifth step, and then the supernatant is put into a glass bottle after cleaning and disinfection for standby;
in the seventh step, taking a proper amount of extracting solution, pouring the extracting solution into the glass flask after the supernatant is taken out in the sixth step again, then repeating the processes in the fourth step and the fifth step, carrying out secondary extraction, carrying out centrifugal layering, sucking the supernatant by using a dropper again after the secondary extraction and the centrifugal layering are finished, and adding the supernatant into the glass flask for later use, thereby finishing the preparation of the grape fern algae extract;
in the eighth step, respectively weighing 45 parts of carbohydrate, 12 parts of bean cake powder, 0.7 part of stabilizer, 0.15 part of emulsifier, 0.3 part of vitamin powder, 1.2 parts of bone meal and 0.06 part of edible pigment according to parts by weight, and then weighing 18 parts of the fiddlehead fern algae extract prepared in the seventh step, wherein the carbohydrate is one or a mixture of corn flour, barley flour and sweet potato flour;
sequentially putting the carbohydrate, the bean cake powder, the stabilizer, the emulsifier, the vitamin powder, the bone meal, the edible pigment and the grape fern algae extract weighed in the step eight into a high-pressure homogenizer for mixing and homogenizing, wherein the working pressure of the high-pressure homogenizer is 40MPa, the working temperature is 65 ℃, the working time is 30min, and the homogenate is prepared for later use after the mixing is finished;
in the tenth step, the homogenate prepared in the ninth step is put into a spray dryer for spray drying, the temperature of the spray dryer is 165 ℃, and raw material particles are prepared for standby after drying;
in the eleventh step, the raw material particles after the spray drying in the tenth step are put into a single-screw extrusion puffing machine for extrusion puffing, and then the raw material particles are added into a granulator for granulation to prepare fish feed particles;
and a twelfth step of vacuum-packaging the fish feed particles prepared in the eleventh step by using a vacuum packaging machine, and then delivering the fish feed particles into a warehouse for storage.
Example 3:
the preparation method of the grape fern extract and the application of the grape fern extract in the formula feed for the aquarium fishes comprise the following steps: step one, cleaning and soaking; step two, drying at high temperature; step three, crushing and sieving; step four, primary extraction; step five, centrifugal layering; step six, separating and storing; step seven, secondary extraction; step eight, weighing raw materials; step nine, mixing and homogenizing; step ten, spray drying; step eleven, puffing and granulating; step twelve, packaging and warehousing;
firstly, taking a proper amount of caulerpa lentillifera, then cleaning dust and impurities on the surface of the caulerpa lentillifera by using distilled water, then soaking the caulerpa lentillifera by using the distilled water for 10min, taking out the caulerpa lentillifera after soaking, and sucking water by using filter paper for later use;
in the second step, the caulerpa lentillifera which is dried in the first step is put into a drying oven for drying until the weight is constant, the caulerpa lentillifera is taken out, the temperature of the drying oven is 105 ℃, the drying time is 2.5 hours, and the caulerpa lentillifera is cooled to room temperature for later use;
in the third step, the dried caulerpa lentillifera obtained in the second step is put into a pulverizer to be pulverized, and the pulverized caulerpa lentillifera is sieved by a 60-mesh sieve, wherein the rotation speed of the pulverizer is 2000r/min, the pulverizing time is 8min, and uniform and fine caulerpa lentillifera powder is left for later use;
in the fourth step, the caulerpa lentillifera powder prepared in the third step is put into a glass flask, then the extracting solution is poured into the glass flask, the extracting solution is water or ethanol, the liquid-material ratio of the extracting solution to the caulerpa lentillifera powder is 30mL/g, and then the glass flask is subjected to ultrasonic-assisted extraction by utilizing ultrasonic waves, wherein the ultrasonic power is 50W, the ultrasonic temperature is 60 ℃, and the ultrasonic time is 11 min;
in the fifth step, after the extraction in the fourth step is finished, putting the glass flask into a centrifuge for centrifugal layering, separating the extract and impurities, centrifuging and standing for later use;
in the sixth step, a dropper is used for sucking out the supernatant in the glass flask after standing and layering in the fifth step, and then the supernatant is put into a glass bottle after cleaning and disinfection for standby;
in the seventh step, taking a proper amount of extracting solution, pouring the extracting solution into the glass flask after the supernatant is taken out in the sixth step again, then repeating the processes in the fourth step and the fifth step, carrying out secondary extraction, carrying out centrifugal layering, sucking the supernatant by using a dropper again after the secondary extraction and the centrifugal layering are finished, and adding the supernatant into the glass flask for later use, thereby finishing the preparation of the grape fern algae extract;
in the eighth step, 50 parts of carbohydrate, 15 parts of bean cake powder, 1 part of stabilizer, 0.5 part of emulsifier, 0.5 part of vitamin powder, 1.5 parts of bone meal and 0.1 part of edible pigment are respectively weighed according to the parts by weight, and then 20 parts of the fiddlehead fern algae extract prepared in the seventh step is weighed, wherein the carbohydrate is one or a mixture of corn flour, barley flour and sweet potato flour;
sequentially putting the carbohydrate, the bean cake powder, the stabilizer, the emulsifier, the vitamin powder, the bone meal, the edible pigment and the grape fern algae extract weighed in the step eight into a high-pressure homogenizer for mixing and homogenizing, wherein the working pressure of the high-pressure homogenizer is 40MPa, the working temperature is 65 ℃, the working time is 30min, and the homogenate is prepared for later use after the mixing is finished;
in the tenth step, the homogenate prepared in the ninth step is put into a spray dryer for spray drying, the temperature of the spray dryer is 165 ℃, and raw material particles are prepared for standby after drying;
in the eleventh step, the raw material particles after the spray drying in the tenth step are put into a single-screw extrusion puffing machine for extrusion puffing, and then the raw material particles are added into a granulator for granulation to prepare fish feed particles;
and a twelfth step of vacuum-packaging the fish feed particles prepared in the eleventh step by using a vacuum packaging machine, and then delivering the fish feed particles into a warehouse for storage.
The properties of the examples are compared in the following table:
based on the above, the invention has the advantages that the invention utilizes the cavitation, shearing and heat effect of ultrasonic waves to increase the permeation between the solvent and the extract, accelerates the extraction speed, greatly increases the extraction efficiency, adopts a physical mode to assist the extraction, reduces the dosage of chemical reagents, is favorable for saving the extraction cost, simultaneously carries out secondary extraction on the residue after the primary extraction, is favorable for improving the extraction degree and the extraction effect, greatly reduces the waste of resources, utilizes the caulerpa viticola extract as the raw material to prepare the fish feed, is favorable for improving the immunologic function of fish, improves the survival rate, effectively prevents the oxidation of fat in the feed, promotes the healing of fish wounds, has the detoxification function, enhances the resistance of fish to the adverse water environment, and has high stability in water, is not easy to dissolve and swell so as to avoid excessive pollution caused by releasing nutrition into water, and is beneficial to environmental protection.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (9)
1. The preparation method of the grape fern extract and the application of the grape fern extract in the formula feed for the aquarium fishes comprise the following steps: step one, cleaning and soaking; step two, drying at high temperature; step three, crushing and sieving; step four, primary extraction; step five, centrifugal layering; step six, separating and storing; step seven, secondary extraction; step eight, weighing raw materials; step nine, mixing and homogenizing; step ten, spray drying; step eleven, puffing and granulating; step twelve, packaging and warehousing; the method is characterized in that:
firstly, taking a proper amount of caulerpa lentillifera, then cleaning dust and impurities on the surface of the caulerpa lentillifera by using distilled water, then soaking the caulerpa lentillifera by using the distilled water, taking out the caulerpa lentillifera after soaking, and sucking water by using filter paper for later use;
in the second step, the caulerpa lentillifera which is dried in the first step is put into a drying box for drying, is taken out after being dried to constant weight, and is cooled to room temperature for later use;
in the third step, the dried caulerpa lentillifera obtained in the second step is put into a grinder to be ground, and the ground caulerpa lentillifera is sieved by a 60-mesh sieve, so that uniform and fine caulerpa lentillifera powder is left for later use;
in the fourth step, the caulerpa lentillifera powder prepared in the third step is put into a glass flask, then the extracting solution is poured into the glass flask, and then the glass flask is subjected to ultrasonic-assisted extraction by utilizing ultrasonic waves;
in the fifth step, after the extraction in the fourth step is finished, putting the glass flask into a centrifuge for centrifugal layering, separating the extract and impurities, centrifuging and standing for later use;
in the sixth step, a dropper is used for sucking out the supernatant in the glass flask after standing and layering in the fifth step, and then the supernatant is put into a glass bottle after cleaning and disinfection for standby;
in the seventh step, taking a proper amount of extracting solution, pouring the extracting solution into the glass flask after the supernatant is taken out in the sixth step again, then repeating the processes in the fourth step and the fifth step, carrying out secondary extraction, carrying out centrifugal layering, sucking the supernatant by using a dropper again after the secondary extraction and the centrifugal layering are finished, and adding the supernatant into the glass flask for later use, thereby finishing the preparation of the grape fern algae extract;
in the eighth step, respectively weighing 40-50 parts by weight of carbohydrate, 10-15 parts by weight of bean cake powder, 0.5-1 part by weight of stabilizer, 0.1-0.5 part by weight of emulsifier, 0.3-0.5 part by weight of vitamin powder, 1-1.5 parts by weight of bone meal and 0.05-0.1 part by weight of edible pigment, and then weighing 15-20 parts by weight of the fiddlehead extract prepared in the seventh step;
sequentially putting the carbohydrate, the bean cake powder, the stabilizer, the emulsifier, the vitamin powder, the bone meal, the edible pigment and the caulerpa vinifera extract weighed in the step eight into a high-pressure homogenizer for mixing and homogenizing, and preparing into homogenate for later use after mixing;
in the above step ten, the homogenate prepared in the step nine is put into a spray dryer for spray drying, and raw material particles are prepared for standby after drying;
in the eleventh step, the raw material particles after the spray drying in the tenth step are put into a single-screw extrusion puffing machine for extrusion puffing, and then the raw material particles are added into a granulator for granulation to prepare fish feed particles;
and a twelfth step of vacuum-packaging the fish feed particles prepared in the eleventh step by using a vacuum packaging machine, and then delivering the fish feed particles into a warehouse for storage.
2. The method for preparing grape fern algae extract and the application thereof in the formula feed for ornamental fish according to claim 1, wherein the method comprises the following steps: in the first step, the soaking time is 5-10 min.
3. The method for preparing grape fern algae extract and the application thereof in the formula feed for ornamental fish according to claim 1, wherein the method comprises the following steps: in the second step, the temperature of the drying box is 105-110 ℃, and the drying time is 2-3 h.
4. The method for preparing grape fern algae extract and the application thereof in the formula feed for ornamental fish according to claim 1, wherein the method comprises the following steps: in the third step, the rotating speed of the pulverizer is 1500-2000r/min, and the pulverizing time is 5-8 min.
5. The method for preparing grape fern algae extract and the application thereof in the formula feed for ornamental fish according to claim 1, wherein the method comprises the following steps: in the fourth step, the extracting solution is water or ethanol, and the liquid-material ratio of the extracting solution to the caulerpa lentillifera powder is 30 mL/g.
6. The method for preparing grape fern algae extract and the application thereof in the formula feed for ornamental fish according to claim 1, wherein the method comprises the following steps: in the fourth step, the ultrasonic power is 50-55W, the ultrasonic temperature is 60-65 ℃, and the ultrasonic time is 11-13 min.
7. The method for preparing grape fern algae extract and the application thereof in the formula feed for ornamental fish according to claim 1, wherein the method comprises the following steps: in the step eight, the carbohydrate is one or a mixture of corn flour, barley flour and sweet potato flour.
8. The method for preparing grape fern algae extract and the application thereof in the formula feed for ornamental fish according to claim 1, wherein the method comprises the following steps: in the ninth step, the working pressure of the high-pressure homogenizer is 30-40MPa, the working temperature is 65-70 ℃, and the working time is 30-40 min.
9. The method for preparing grape fern algae extract and the application thereof in the formula feed for ornamental fish according to claim 1, wherein the method comprises the following steps: in the step ten, the temperature of the spray dryer is 160-170 ℃.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960563A (en) * | 2012-11-15 | 2013-03-13 | 江西金龟王实业有限责任公司 | Aquarium fish feed |
| CN104397449A (en) * | 2014-12-11 | 2015-03-11 | 青岛德润电池材料有限公司 | Nutritious feed for aquarium fish and preparation method for the feed |
| CN107173636A (en) * | 2017-06-07 | 2017-09-19 | 天津市晨辉饲料有限公司 | A kind of fancy carp feed and preparation method thereof |
| CN109833334A (en) * | 2018-12-13 | 2019-06-04 | 香港科技大学 | The preparation method and application of long shoot grape caulerpa extract |
| AU2020102960A4 (en) * | 2020-10-22 | 2021-01-21 | Yellow Sea Fisheries Research Institute, Chinese Academy Of Fishery Sciences | A Breeding method of polyculture of Panulirus ornatus and Caulerpa lentillifera |
-
2021
- 2021-06-25 CN CN202110710256.1A patent/CN113412890A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960563A (en) * | 2012-11-15 | 2013-03-13 | 江西金龟王实业有限责任公司 | Aquarium fish feed |
| CN104397449A (en) * | 2014-12-11 | 2015-03-11 | 青岛德润电池材料有限公司 | Nutritious feed for aquarium fish and preparation method for the feed |
| CN107173636A (en) * | 2017-06-07 | 2017-09-19 | 天津市晨辉饲料有限公司 | A kind of fancy carp feed and preparation method thereof |
| CN109833334A (en) * | 2018-12-13 | 2019-06-04 | 香港科技大学 | The preparation method and application of long shoot grape caulerpa extract |
| AU2020102960A4 (en) * | 2020-10-22 | 2021-01-21 | Yellow Sea Fisheries Research Institute, Chinese Academy Of Fishery Sciences | A Breeding method of polyculture of Panulirus ornatus and Caulerpa lentillifera |
Non-Patent Citations (3)
| Title |
|---|
| WF YAP等: "Decoding Antioxidant and Antibacterial Potentials of Malaysian Green Seaweeds: Caulerpa racemosa and Caulerpa lentillifera", 《ANTIBIOTICS》, vol. 8, no. 3, 31 December 2019 (2019-12-31), pages 1 - 18 * |
| 张媚健等: "海南长茎葡萄蕨藻营养成分分析及其免疫刺激活性研究", 《食品科技》, no. 05, 31 December 2019 (2019-12-31), pages 90 - 96 * |
| 曾广厅等: "观赏鱼的营养与饲料配制", 《饲料广角》, no. 15, 31 December 2004 (2004-12-31), pages 40 - 42 * |
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