CN113412247B - Agricultural preparation containing polyglutamic acid and amino-oligosaccharin - Google Patents
Agricultural preparation containing polyglutamic acid and amino-oligosaccharin Download PDFInfo
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- CN113412247B CN113412247B CN201980088901.1A CN201980088901A CN113412247B CN 113412247 B CN113412247 B CN 113412247B CN 201980088901 A CN201980088901 A CN 201980088901A CN 113412247 B CN113412247 B CN 113412247B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
An agricultural preparation containing polyglutamic acid and amino-oligosaccharin is disclosed, which is a solution type preparation, and the active ingredients comprise 5-35 g/L polyglutamic acid, 8-15 g/L amino-oligosaccharin, 0.5-1.5 g/L abelmoschus manihot gum, 80-120 g/L organic matter, medium trace elements chelated zinc and chelated calcium. The agricultural preparation can kill pathogenic bacteria, improve crop stress resistance, increase yield, improve quality, improve fertilizer utilization rate, remould soil granular structure, cooperate fertilizer to increase efficiency and cooperate pesticide to increase efficiency by adjusting the proportion of organic matters, chelated zinc, chelated calcium, polyglutamic acid, amino-oligosaccharin and abelmoschus manihot gum. In addition, the polyglutamic acid is prepared by microbial fermentation, is rich in amino acid, vitamins and other nutritional ingredients, and can enhance the growth promoting effect of crops when being used as a biostimulant.
Description
Technical Field
The invention belongs to the field of agriculture, and particularly relates to an agricultural preparation containing polyglutamic acid and amino-oligosaccharin (also called chitosan oligosaccharide).
Background
China is a big agricultural country, farmland soil is the basis of agricultural development, and a large amount of fertilizer is used, so that outstanding contribution is made to agricultural development and grain yield increase. The use of fertilizers also presents a number of negative problems. The utilization rate of the fertilizer is low, and the phenomena of soil hardening, soil-borne diseases, salinization, water body pollution and the like threaten the sustainable development of agriculture to a great extent, and the development of a novel agricultural preparation which is efficient, ecological, specialized, simple and convenient and multifunctional is an inevitable trend and a development trend of modern agriculture and a prospect product of the sustainable development of future agriculture. The existing fertilizer has the problems of low utilization rate, large using amount, soil eutrophication and unfavorable growth of crops.
Disclosure of Invention
The invention aims to solve the technical problem of providing an agricultural preparation containing polyglutamic acid and amino-oligosaccharin, which can improve the utilization rate of fertilizer and effectively improve the yield of crops aiming at the defects of the prior art.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
an agricultural preparation containing polyglutamic acid and amino-oligosaccharin, which is a solution type preparation, the solvent is water, and the active ingredients with the following concentrations are contained:
the preferable range of the formula is as follows:
wherein, the polyglutamic acid is any one or the combination of a plurality of gamma-polyglutamic acid pure products, gamma-polyglutamic acid fermentation liquor or gamma-polyglutamic acid fermentation liquor spray drying powder; wherein the molecular weight of the polyglutamic acid is 5000-100000 Da, preferably 50000E100000Da. The polyglutamic acid of the present invention can be prepared by the prior art or purchased directly from the market. Gamma-polyglutamic acid is a kind of homo-polyamino acid produced by microbial fermentation, and contains terminal amine and multiple COO - Ca in group, soil and fertilizer 2+ 、Mg 2+ 、Zn 2+ The polyglutamic acid can be directly bonded with the carboxyl site and the amido site of the polyglutamic acid to form water-soluble and stable polyglutamate, and the water-soluble and stable polyglutamate can slowly provide nutrition for the growth of crops. Meanwhile, the special polycarboxyl structure has super-strong water absorption and retention capacity, can reduce the loss of water and fertilizer in soil and can remold a soil aggregate mechanism. Improve the photosynthesis efficiency and the stress resistance of crops, increase the yield and improve the quality of crops.
The amino-oligosaccharin is any one or the combination of two of solution and powder, and the polymerization degree of the amino-oligosaccharin is 4-6. The amino-oligosaccharins according to the present invention may be either self-made according to the prior art or purchased directly from the market. The amino-oligosaccharin (chitosan oligosaccharide) is oligosaccharide with D-glucosamine connected by beta-1, 4 glycosidic bond, and is prepared by degrading chitosan with chitin, or by extracting with microorganism fermentation. The amino-oligosaccharin (agricultural-grade chitosan oligosaccharide) can inhibit the growth of some pathogenic bacteria, influence fungal spore germination, induce hyphal morphological variation, spore biochemical change and the like. Can excite the gene in the plant body, produce chitinase, glucanase, preservative, PR protein and the like with disease-resistant function, has the function of activating cells, is beneficial to the recovery of the damaged plant, promotes the roots and the seedlings, enhances the stress resistance of crops, and promotes the growth and the development of the plants. The amino-oligosaccharin solution has the functions of killing virus, bacteria and fungi. The application has the characteristics of trace (PPM level), high efficiency, low cost, no pollution and the like.
The abelmoschus manihot gum is powder, is prepared from roots and stems of abelmoschus manihot through sorting, cleaning, drying, chopping, grinding, sieving and preparing, and can be directly purchased from the market. The abelmoschus manihot gum has good water retention and thickening effects, and keeps stable performance of the agricultural preparation.
The organic matter is any one or combination of biochemical fulvic acid and glucose.
The biochemical fulvic acid is prepared by concentrating supernatant of saccharomycete fermented molasses liquid. The biochemical fulvic acid has the characteristics of improving soil, increasing the utilization rate of fertilizer, stimulating plant growth, improving the quality of agricultural products, enhancing the stress resistance of crops and the like.
When the organic matter is a combination of two substances, namely, biochemical fulvic acid and glucose, the proportion of the two substances is not particularly limited in the invention, as long as the organic matter contains the two substances.
The components of polyglutamic acid, amino-oligosaccharin, abelmoschus manihot gum, biochemical fulvic acid, chelated zinc and chelated calcium have a synergistic effect, and the effects of agricultural preparations can be changed by adjusting the proportion of the biochemical fulvic acid, the chelated zinc and the chelated calcium to the polyglutamic acid, the amino-oligosaccharin and the abelmoschus manihot gum, for example, the growth of leaf vegetables can be improved by increasing the concentration of the chelated zinc, and the melon and fruit crops with the concentration of the chelated calcium are more prominent in performance.
The preparation method of the agricultural preparation containing polyglutamic acid and amino-oligosaccharin comprises the following steps: mixing polyglutamic acid, amino-oligosaccharin, abelmoschus manihot gum, organic matter, chelated zinc, chelated calcium and the like.
Wherein, if the organic matter contains the biochemical fulvic acid, the biochemical fulvic acid is added after other raw materials are mixed.
The application of the agricultural preparation containing polyglutamic acid and amino-oligosaccharin as fertilizer is also within the protection scope of the invention.
The fertilizer is applied to field crops and economic crops in a flushing or spraying mode, the flushing application amount is 10-800 g/mu, the spraying amount is 5-400 g/mu, and the application time is the whole growth period of the crops.
The field crop may be, for example, wheat, rice or corn.
The commercial crop can be fruits or vegetables and the like.
Has the beneficial effects that:
1. the agricultural preparation improves the utilization rate of the fertilizer and effectively improves the crop yield by adjusting the proportion of the biochemical fulvic acid, the chelated zinc, the chelated calcium, the polyglutamic acid, the amino-oligosaccharin and the abelmoschus manihot gum.
2. The fermentation liquor of the polyglutamic acid is rich in amino acid, vitamin and other nutrient components besides the polyglutamic acid, can promote the absorption of trace elements of crops, and can improve the growth promoting effect of fertilizers on the crops and effectively improve the quality of the crops by using the components as biostimulant.
3. The polyglutamic acid, the amino-oligosaccharin, the abelmoschus manihot gum and the biochemical fulvic acid in the agricultural preparation formula are all biological products, can be completely biodegraded in soil, are harmless to human bodies and soil organisms, and have no pollution to soil environment.
Detailed Description
The invention will be better understood from the following examples.
In the following examples, some of the components were obtained by the following methods.
Preparation of polyglutamic acid: treating polyglutamic acid fermentation liquor, adding concentrated sulfuric acid to adjust the pH value to about 3.5, heating to 70-90 ℃, preserving heat for 0.5-1.5 hours, then adjusting the pH value to 5.0-8.0 by using sodium hydroxide, selecting diatomite and active carbon to be combined, and carrying out adsorption treatment on the treatment liquid to obtain the polyglutamic acid fermentation liquor with the molecular weight of 5000-100000 Da;
preparation of amino-oligosaccharin: adding the crude enzyme preparation prepared by fermenting escherichia coli into the chitosan solution, and reacting for 6-12 hours at 40-60 ℃. The reaction mixture is subjected to enzyme deactivation and activation to obtain a concentrated solution of the amino-oligosaccharide, and the concentrated solution is subjected to separation and purification through ultrafiltration, nanofiltration and the like to obtain an amino-oligosaccharide solution with the polymerization degree of 4-6;
preparing biochemical fulvic acid: fermenting the molasses liquid by saccharomycetes, culturing to obtain a supernatant, and concentrating the supernatant by 4-5 times to obtain the biochemical fulvic acid, namely preparing the biochemical fulvic acid.
Example 1:
the agricultural preparation containing polyglutamic acid and amino-oligosaccharin is prepared by mixing polyglutamic acid, amino-oligosaccharin, abelmoschus manihot gum, water and biochemical fulvic acid. Wherein the concentration of polyglutamic acid (molecular weight of 50000 Da) is 5g/L, the concentration of amino-oligosaccharin (polymerization degree of 4) is 8g/L, the concentration of abelmoschus manihot gum is 0.5g/L, and the concentration of biochemical fulvic acid is 80g/L.
Example 2:
the agricultural preparation containing polyglutamic acid and amino-oligosaccharin is prepared by mixing polyglutamic acid, amino-oligosaccharin, abelmoschus manihot gum, chelated zinc, chelated calcium, water and biochemical fulvic acid. Wherein the concentration of polyglutamic acid (molecular weight is 80000 Da) is 20g/L, the concentration of amino-oligosaccharin (polymerization degree is 5) is 10g/L, the concentration of abelmoschus manihot gum is 1g/L, the concentration of biochemical fulvic acid is 100g/L, the concentration of chelated zinc is 5g/L, and the concentration of chelated calcium is 6g/L.
Example 3:
the agricultural preparation containing polyglutamic acid and amino-oligosaccharin is prepared by mixing polyglutamic acid, amino-oligosaccharin, abelmoschus manihot gum, chelated zinc, chelated calcium, water, glucose and biochemical fulvic acid. Wherein the concentration of polyglutamic acid (molecular weight is 100000 Da) is 35g/L, the concentration of amino-oligosaccharin (polymerization degree is 6) is 15g/L, the concentration of abelmoschus manihot gum is 1.5g/L, the concentration of glucose is 20g/L, the concentration of biochemical fulvic acid is 100g/L, the concentration of chelated zinc is 10g/L, and the concentration of chelated calcium is 10g/L.
Example 4: the influence of the agricultural preparation on the yield structure of the pakchoi
In order to investigate the influence of the agricultural preparation of the invention on the yield structure of the pakchoi, 3 treatments are designed in the experiment:
treatment 1: no fertilizer is applied;
and (3) treatment 2: applying organic fertilizer and compound fertilizer (15-15-15);
and (3) treatment: applying an organic fertilizer, a compound fertilizer (15-15-15) and an agricultural preparation containing gamma-polyglutamic acid and amino-oligosaccharin (prepared in example 1);
in this embodiment, the organic fertilizer is a conventional organic fertilizer (N + P) on the market 2 O 5 +K 2 O is more than or equal to 5 percent, organic matter is more than or equal to 45 percent), and the compound fertilizer (15-15-15) is a conventional compound fertilizer in the market.
This example is a pakchoi plot experiment,the variety is four-season pakchoi, the test is repeated for 3 times, 9 cells are counted, and the cell area is 5m multiplied by 4m =20m 2 And the cells are randomly distributed. Treatment 1 did not apply fertilizer. And treating 2 mu of land, applying 300kg of organic fertilizer and 20kg of compound fertilizer as base fertilizer, and ploughing the soil after applying the fertilizer. Treating 3 the base fertilizer, applying the same as treating 2, and spraying an agricultural preparation containing polyglutamic acid and amino-oligosaccharin with the dosage of 5L/mu.
The test pakchoi was randomly sampled, and indicators such as plant height, root length, single plant weight and the like were selected for investigation, as shown in table 1.
TABLE 1 Effect of agricultural formulations on the yield structure of Brassica rapa
As can be seen from Table 1, the plant height of treatment 3 was increased by 7.2cm as compared with treatment 1 and by 2.1cm as compared with treatment 2; the root length of the treatment 3 is increased by 3cm compared with the treatment 1 and increased by 1cm compared with the treatment 2; the weight of the individual plant was increased by 42.7g in treatment 3 and 14.1g in treatment 2. The embodiment shows that the agricultural preparation containing polyglutamic acid and amino-oligosaccharin has good promotion effect on growth of the crop of the Chinese cabbage, and the yield increasing effect is obvious.
Example 5: effect of the agricultural formulations of the present invention on tomato yield structure
To investigate the effect of the agricultural formulation of the present invention on the tomato yield structure, the experiment designed 3 treatments:
treatment 1: no fertilizer is applied;
and (3) treatment 2: applying organic fertilizer and compound fertilizer (18-18-18);
and (3) treatment: applying an organic fertilizer, a compound fertilizer (18-18-18) and an agricultural preparation containing polyglutamic acid and amino-oligosaccharin (prepared in example 2);
in this embodiment, the organic fertilizer is a conventional organic fertilizer (N + P) on the market 2 O 5 +K 2 O is more than or equal to 5 percent, organic matter is more than or equal to 45 percent), and the compound fertilizer (18-18-18) is a conventional compound fertilizer in the market.
This example is a tomato plot experiment of speciesAnd 5m x 4m =20m cell area, namely, 5 times of repeated experiments are carried out for 3 times, and 9 cells are counted 2 And the cells are randomly distributed. Treatment 1 did not apply fertilizer. And treating 2 mu of land, applying 500kg of organic fertilizer and 50kg of compound fertilizer as base fertilizer, and ploughing the soil after applying the fertilizer. And 3, performing flushing application on an agricultural preparation containing polyglutamic acid and amino-oligosaccharin once every 7-10 days after the tomatoes are transplanted, performing flushing application for 3 times in the whole growth period, wherein the using amount is 5L/mu, and performing base fertilizer application in the same way as in 2.
The test tomatoes were sampled randomly, and indexes such as sugar content and individual plant fruit weight were selected for examination, as shown in table 2.
TABLE 2 Effect of application of agricultural formulations on tomato sugar content, weight of individual fruits
As is clear from Table 2, the sugar content in treatment 3 was increased by 0.88% as compared with treatment 1 and by 0.55% as compared with treatment 2; the weight of the individual fruit was increased by 569.7g compared with treatment 1 and 296.2g compared with treatment 2. According to the embodiment, the agricultural preparation containing polyglutamic acid and amino-oligosaccharin has the advantages that the taste quality of the tomato is improved, the yield increasing effect is obvious, the tomato is robust in the whole growth period, and diseases are not easily infected.
Example 6: effect of the agricultural formulations of the present invention on Potato yield Structure
To investigate the effect of the agricultural formulation of the present invention on potato yield structure, 3 treatments were designed experimentally:
treatment 1: no fertilizer is applied;
and (3) treatment 2: applying organic fertilizer and compound fertilizer (15-15-15);
and (3) treatment: applying an organic fertilizer, a compound fertilizer (15-15-15) and an agricultural preparation containing polyglutamic acid and amino-oligosaccharin (prepared in example 3);
the organic fertilizer described in this embodiment is a conventional organic fertilizer (N + P) on the market 2 O 5 +K 2 O is more than or equal to 5 percent, organic matter is more than or equal to 45 percent), and the compound fertilizer (15-15-15) is a conventional compound fertilizer in the market.
This example is a potato cell test, the variety is wannon No. 4, the test is repeated 3 times, 9 cells are counted, and the cell area is 6 mx 4m =24m 2 Each cell is randomly distributed. Each cell is randomly distributed. Treatment 1 did not apply fertilizer. 800kg of organic fertilizer and 50kg of compound fertilizer are applied to 2 mu of potato field planting, soil is ploughed after the fertilizer is applied, dimethomorph is used for preventing epidemic disease during potato field planting, and macroelement water-soluble fertilizer is additionally applied in the full-bloom stage and the expanding stage, and the using amount is 10 kg/mu. When 3 potatoes are treated for field planting, an agricultural preparation of polyglutamic acid and amino-oligosaccharin is used, the dosage is 10L/mu, the full-bloom stage and the expansion stage are applied by matching with macroelement water-soluble fertilizer, the dosage is 5L/mu, the agricultural preparation is applied for 3 times in the whole growth period, and the other applications are the same as the treatment 2. The test potatoes were randomly sampled and selected for examination as shown in table 3.
TABLE 3 Effect of the application of the agricultural formulation on the weight and height of the potato plants
As can be seen from Table 3, the weight of the potato plant of treatment 3 was increased by 188.3g as compared with treatment 1 and by 68.0g as compared with treatment 2; the plant height of the individual plant 3 was increased by 20.2cm compared with that of treatment 1 and by 5.8cm compared with that of treatment 2. The present example shows that the agricultural preparation containing polyglutamic acid and amino-oligosaccharin of the present invention has an excellent disease prevention effect on potatoes, good plant growth, and an obvious potato yield increase effect.
Example 7: effect of the agricultural formulations of the present invention on cucumber yield Structure
In order to investigate the influence of the agricultural formulation of the present invention on the cucumber yield structure, 3 treatments were designed for the experiment:
treatment 1: no fertilizer is applied;
and (3) treatment 2: applying organic fertilizer and compound fertilizer (17-17-17);
and (3) treatment: applying an organic fertilizer, a compound fertilizer (17-17-17) and an agricultural preparation containing polyglutamic acid and amino-oligosaccharin (prepared in example 2);
in the embodiment, the organic fertilizer is conventional organic fertilizer (N + P) on the market 2 O 5 +K 2 O is more than or equal to 5 percent, organic matter is more than or equal to 45 percent), and the compound fertilizer (17-17-17) is a conventional compound fertilizer in the market.
This example is a cucumber cell test, the variety is jin you No. 5, the test is repeated 3 times, the total number is 9 cells, and the cell area is 5m × 4m =20m 2 And the cells are randomly distributed. Treatment 1 did not apply fertilizer. And treating 2 mu of land, applying 1000kg of organic fertilizer and 50kg of compound fertilizer as base fertilizer, and ploughing the soil after applying the fertilizer. And 3, performing flushing application on an agricultural preparation containing polyglutamic acid and amino-oligosaccharin once every 7-10 days after the cucumber is transplanted, performing flushing application for 3 times in the whole growth period, wherein the using amount is 10L/mu, and performing base fertilizer application in the same way as in the step 2. The test cucumber was sampled randomly, and the individual cucumber weight and cucumber length indices were selected for examination, as shown in table 4.
TABLE 4 Effect of the application of agricultural formulations on cucumber yield structure
As can be seen from Table 4, the weight of the individual fruit of treatment 3 was increased 375.4g as compared with treatment 1 and 169.4g as compared with treatment 2; the melon growth treatment 3 is increased by 12cm compared with the treatment 1 and is increased by 4.3cm compared with the treatment 2. According to the embodiment, the agricultural preparation containing polyglutamic acid and amino-oligosaccharin has an obvious effect of increasing the yield of cucumber, good fruit type and commodity, leaves of cucumber plants are dark green and thick, the plants grow vigorously, plant diseases and insect pests such as root-knot nematodes are obviously reduced in the whole growth period, the picking period can be prolonged by more than 15 days, and the yield can be further improved.
Example 8: influence of agricultural preparation, single component, two components and three components on yield structure of pakchoi
The influence of the agricultural preparation, the single component, the two components and the three components on the yield structure of the pakchoi is inspected, and in other plot arrangement tests, 16 treatments are designed:
treatment 1: conventional fertilization;
and (3) treatment 2: applying fertilizers conventionally, and spraying agricultural preparations (prepared in example 1) containing gamma-polyglutamic acid and amino-oligosaccharin, wherein the dosage per mu is 5L;
and (3) treatment: applying fertilizers conventionally, spraying 5g/L polyglutamic acid, and using 5L per mu;
and (4) treatment: applying fertilizer conventionally, spraying 8g/L amino-oligosaccharin, and using 5L per mu;
and (4) treatment 5: applying fertilizer conventionally, spraying 0.5g/L of abelmoschus manihot gum, and using 5L per mu;
and (6) treatment: applying fertilizer conventionally, and spraying 80g/L biochemical fulvic acid with 5L dosage per mu;
and (7) treatment: applying fertilizers conventionally, namely applying 5g/L polyglutamic acid and 8g/L amino-oligosaccharin with the dosage of 5L per mu;
and (4) treatment 8: applying fertilizers conventionally, namely applying 8g/L amino-oligosaccharin and 0.5g/L abelmoschus manihot gum by water, wherein the dosage per mu is 5L;
and (9) processing: applying fertilizers conventionally, namely applying 0.5g/L of abelmoschus manihot gum and 5g/L of polyglutamic acid with the dosage of 5L per mu;
and (4) processing 10: applying the fertilizer conventionally, namely applying 80g/L of biochemical fulvic acid and 5g/L of polyglutamic acid by flushing, wherein the dosage per mu is 5L;
and (3) treatment 11: applying fertilizers conventionally, namely applying 0.5g/L of abelmoschus manihot gum and 80g/L of biochemical fulvic acid with the dosage of 5L per mu;
and (4) treatment 12: applying the fertilizer conventionally, namely applying 80g/L of biochemical fulvic acid and 8g/L of amino-oligosaccharin by flushing, wherein the dosage per mu is 5L;
and (4) treatment: applying 5g/L polyglutamic acid, 8g/L amino-oligosaccharin and 0.5g/L abelmoschus manihot gum by conventional fertilization, wherein the dosage per mu is 5L;
and (4) processing: applying 8g/L amino-oligosaccharin, 5g/L polyglutamic acid and 80g/L biochemical fulvic acid by conventional fertilization, wherein the dosage per mu is 5L;
and (4) processing: applying fertilizer conventionally, namely applying 0.5g/L of abelmoschus manihot gum, 80g/L of biochemical fulvic acid and 5g/L of polyglutamic acid with the dosage of 5L per mu;
and (3) treatment 16: applying the fertilizer by a conventional method, wherein 80g/L of biochemical fulvic acid, 0.5g/L of abelmoschus manihot gum and 8g/L of amino-oligosaccharin are applied by water, and the dosage per mu is 5L.
The conventional fertilization is to apply 300kg of organic fertilizer and 20kg of compound fertilizer (15-15-15) as base fertilizer, wherein the molecular weight of polyglutamic acid used in the treatment is 50000Da, and the polymerization degree of amino-oligosaccharin is 4.
This example is a small-area test of Chinese cabbage, the variety is four season Chinese cabbage, the test is designed for 3 times of repetition, 48 cells are counted, and the cell area is 2m × 3m =6m 2 And the cells are randomly distributed.
The test pakchoi was sampled randomly and the indexes such as plant height, root length, individual plant weight, etc. were selected for investigation as shown in table 5.
TABLE 5 Effect of the agricultural formulation, single ingredient, two ingredients and three ingredients on the yield structure of pakchoi
As can be seen from Table 5, the plant height of treatment 2 was increased by 8.9cm as compared with treatment 1, the root length was increased by 3.4cm as compared with treatment 2 as compared with treatment 1, and the weight of each plant was increased by 39.0g as compared with treatment 2; treatment 2 performed better than the other treatments. From the overall data, compared with the single component, the two components and the three components in the formula components, the agricultural preparation containing polyglutamic acid and amino-oligosaccharin has better performance on plant height and root length of the pakchoi, and has obvious yield increasing effect. The four components of polyglutamic acid, amino-oligosaccharin, biochemical fulvic acid and abelmoschus manihot gum have good synergistic effect.
The present invention provides a method and a device for preparing agricultural formulations containing polyglutamic acid and amino-oligosaccharins, and the method and the device for implementing the technical scheme are many, and the above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and embellishments can be made without departing from the principle of the present invention, and these modifications and embellishments should also be regarded as the protection scope of the present invention. All the components not specified in the present embodiment can be realized by the prior art.
Claims (3)
1. An agricultural formulation comprising polyglutamic acid and amino-oligosaccharins, characterized in that it is a solution-type formulation, the solvent is water, and it contains the following active ingredients in the following concentrations:
5 to 35g/L of polyglutamic acid,
8 to 15g/L of amino-oligosaccharin,
0.5 to 1.5g/L of abelmoschus manihot gum,
the content of the organic matter is 80 to 120g/L,
0 to 10g/L of chelated zinc,
0 to 10g/L of chelated calcium;
the polyglutamic acid is any one or combination of more of a gamma-polyglutamic acid pure product, a gamma-polyglutamate pure product, gamma-polyglutamic acid fermentation liquor or gamma-polyglutamic acid fermentation liquor spray drying powder;
the molecular weight of the polyglutamic acid is 5000 to 100000Da;
the polymerization degree of the amino-oligosaccharin is 4 to 6;
the organic matter is any one or combination of two of biochemical fulvic acid and glucose;
the biochemical fulvic acid is prepared by concentrating supernatant of saccharomycete fermented molasses liquid;
the pH value of the agricultural preparation is 5.0 to 7.0, and the water-insoluble substance is less than or equal to 20 g/L.
2. Use of an agricultural formulation comprising polyglutamic acid and an amino-oligosaccharin according to claim 1 as a fertilizer.
3. The application of the fertilizer as claimed in claim 2, wherein the fertilizer is applied to field crops and economic crops in a mode of flushing or spraying, the flushing application rate is 10-800 g/mu, the spraying dosage is 5-400 g/mu, and the application time is the whole growth period of the crops.
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| CN2019101446756 | 2019-02-27 | ||
| PCT/CN2019/105620 WO2020173079A1 (en) | 2019-02-27 | 2019-09-12 | Agricultural preparation containing polyglutamic acid and amino-oligosaccharin |
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| CN109824430A (en) * | 2019-02-27 | 2019-05-31 | 南京轩凯生物科技有限公司 | A kind of Agrotechnical formulation containing polyglutamic acid and amino-oligosaccharide |
| CN110194705A (en) * | 2019-07-02 | 2019-09-03 | 轩凯生物科技(滁州)有限公司 | A kind of diammonium phosphate compound fertilizer and the preparation method and application thereof |
| CN110903144A (en) * | 2019-12-02 | 2020-03-24 | 黄河三角洲京博化工研究院有限公司 | Polyglutamic acid chelated medium element preparation and preparation method thereof |
| CN111205136A (en) * | 2020-03-09 | 2020-05-29 | 杜得信 | Soil conditioner containing sodium glutamate and amino-oligosaccharin |
| CN111742929A (en) * | 2020-08-07 | 2020-10-09 | 南京轩凯生物科技有限公司 | Synergistic insecticide and application thereof |
| CN112194523A (en) * | 2020-09-24 | 2021-01-08 | 石家庄农信生物科技集团有限公司 | Plant immune preparation containing fulvic acid and chitosan oligosaccharide |
| CN112919960A (en) * | 2020-12-31 | 2021-06-08 | 河北省农林科学院农业资源环境研究所 | High-efficiency functional fertilizer for tomato in seedling stage and preparation process thereof |
| CN113816788A (en) * | 2021-08-11 | 2021-12-21 | 安徽朴茂农业科技有限公司 | Powdered biological film fertilizer water retaining agent |
| CN115745709A (en) * | 2022-12-27 | 2023-03-07 | 河南帮丰农业科技有限公司 | Compound fertilizer and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104945142A (en) * | 2015-06-29 | 2015-09-30 | 山东卫康生物医药科技有限公司 | Special chitosan oligosaccharide containing multi-trace-element foliar fertilizer for leaf vegetables and preparation method thereof |
| CN107235765A (en) * | 2017-06-09 | 2017-10-10 | 谢媛 | One plant growth regulators |
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| CN103896672A (en) * | 2012-12-29 | 2014-07-02 | 中国科学院沈阳应用生态研究所 | Polyion synergia stabilized urea ammonium nitrogen fertilizer and preparation method thereof |
| CN105230661B (en) * | 2015-10-10 | 2018-05-25 | 党永富 | Crop functional aid and preparation method thereof |
| CN107162823A (en) * | 2017-07-19 | 2017-09-15 | 山东美吉多生态农业科技有限公司 | A kind of preparation and application of poly-aspartate Liquid Fertilizer |
| CN109824430A (en) * | 2019-02-27 | 2019-05-31 | 南京轩凯生物科技有限公司 | A kind of Agrotechnical formulation containing polyglutamic acid and amino-oligosaccharide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104945142A (en) * | 2015-06-29 | 2015-09-30 | 山东卫康生物医药科技有限公司 | Special chitosan oligosaccharide containing multi-trace-element foliar fertilizer for leaf vegetables and preparation method thereof |
| CN107235765A (en) * | 2017-06-09 | 2017-10-10 | 谢媛 | One plant growth regulators |
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| CN113412247A (en) | 2021-09-17 |
| WO2020173079A1 (en) | 2020-09-03 |
| CN109824430A (en) | 2019-05-31 |
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