CN113406048B - Fluorescence excitation type detection pen - Google Patents
Fluorescence excitation type detection pen Download PDFInfo
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- CN113406048B CN113406048B CN202110653463.8A CN202110653463A CN113406048B CN 113406048 B CN113406048 B CN 113406048B CN 202110653463 A CN202110653463 A CN 202110653463A CN 113406048 B CN113406048 B CN 113406048B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
Abstract
The invention relates to a fluorescence excitation type detection pen, which is characterized in that a light source with preset wavelength is arranged near a detection window in the detection pen, a reagent strip with higher sensitivity, better specificity and wider detection linearity is selected to replace a colloidal gold product, and under the condition that the concentration of fluorescent substances in a detected substance is smaller, the fluorescent substances with low concentration can be excited to emit fluorescence through the irradiation of the light source, so that the fluorescence excitation type detection pen can be directly observed by human eyes, and the detection pen has higher detection precision, wider detectable concentration range and better universality.
Description
Technical Field
The invention belongs to the technical field of medical appliances, and relates to a fluorescence excitation type detection pen.
Background
The fluorescent substances on the test paper in the traditional test pen must reach a certain concentration to be visible to naked eyes. Therefore, the analyte needs to reach a certain concentration to cause the fluorescent substance to react to generate fluorescence. The existing detection pen or detection test paper on the market mainly uses a colloidal gold method, when the detection pen or the detection test paper is used in occasions such as early pregnancy, the result cannot be accurately detected once the HCG concentration is lower than a certain degree, and the colloidal gold has no anti-interference capability and other fluorescent materials such as common fluorescent microspheres, quantum dot fluorescent microspheres and the like.
The reagent products adopting the colloidal gold method are mostly used for judging results by visual inspection of colors by naked eyes, and the judgment of the color identification has subjectivity to a certain extent, particularly, the judgment of the results near a critical value can cause interpretation trouble to customers, and the fluorescent reagent has higher sensitivity, compared with the current colloidal gold products, the sensitivity is higher, the judgment of the detection results near the critical value is more advantageous, the reflection signal is more excellent, and the color identification is replaced by fluorescence, so that the judgment of the color identification is easier for naked eyes.
Chinese patent document CN107356767a discloses a luteinizing hormone test strip analyzer, which comprises a reagent card and a detection pen; the detection pen comprises a shell, a shading cavity arranged in the shell and a photoelectric detection system for judging the color depth, wherein an insertion port is formed in the shell of the detection pen, and when the reagent card is inserted into the detection pen through the insertion port, the detection window is correspondingly inserted into the shading cavity. The photoelectric detection system is used for collecting reflected light in the color shade change process of the test strip, converting an optical signal into a digital electric signal, automatically judging and obtaining a detection result according to threshold comparison, so that the accuracy and the measurement efficiency of the measurement result are improved, non-contact detection is realized, pollution to a detection pen is avoided, meanwhile, the detection pen can be recycled through the split design of the reagent card and the detection pen, and the detection cost is saved.
Chinese patent document CN102033129a discloses a test pen for detecting pathogenic microorganisms, comprising blood filtering paper, colloidal gold pad, nitrocellulose membrane, water absorbing filter paper and PVC base plate; the colloidal gold pad is coated with a monoclonal antibody of mouse anti-human INF-gamma, namely a colloidal gold compound, the nitrocellulose membrane is provided with a test line and a control line, the test line is coated with IFN-gamma, the control line is coated with a goat anti-mouse IgG polyclonal antibody, after a sample is dripped into a water absorption rod, IFN-gamma in the sample moves onto the colloidal gold pad along with the solution and is combined with the gold-labeled IFN-gamma monoclonal antibody in the sample to form an antigen-antibody compound, and if the IFN-gamma in the sample is insufficient, the gold-labeled IFN-gamma monoclonal antibody can be combined with antigen IFN-gamma again, the monoclonal antibodies can be combined with IFN-gamma at a T line to make the T line red along with the movement of the solution to the T line; conversely, if IFN-gamma in the sample is in excess such that the gold-labeled IFN-gamma monoclonal antibodies are no longer able to bind the antigen IFN-gamma, these monoclonal antibodies will not bind IFN-gamma at the T-line as the solution moves to the T-line, which will appear in the absence of color. The gold-labeled IFN-gamma monoclonal antibody which is not combined with the T line continuously moves to the C line along with the solution, and the C line is red due to the combination of the gold-labeled IFN-gamma monoclonal antibody and the goat anti-mouse polyclonal antibody at the C line, which suggests that the mouse anti-human IFN-gamma monoclonal antibody can be recognized by the goat anti-mouse polyclonal antibody, but the nitrocellulose membrane adopted in the structure also can be recognized by judging the color change to obtain the detection result, and has the requirement on the concentration of the detected object in the sample, and once the concentration of the detected object is too low, the color change of the nitrocellulose membrane can be difficult for naked eyes to observe.
Disclosure of Invention
Aiming at the problems in the prior art, the invention discloses a fluorescence excitation type detection pen, wherein a light source with preset wavelength is arranged near a detection window in the detection pen, and a reagent strip with higher sensitivity, better specificity and wider detection linearity is selected to replace a colloidal gold product.
The technical scheme adopted by the invention for solving the technical problems is as follows: a fluorescence excitation type detection pen, comprising:
the detection shell is integrally divided into a shell body, a shell head and a shell tail, wherein the detection shell comprises a first shell and a second shell, the first shell and the second shell are detachably connected, and an accommodating space is arranged between the first shell and the second shell; the first shell is provided with a detection window corresponding to the shell body, and the first shell is provided with a collection hole corresponding to the shell head;
the test paper assembly comprises a water absorption part, a detection part, a collection part and a bearing substrate, wherein the bearing substrate is of a strip-shaped plate structure, one part of the bearing substrate is positioned at the shell part of the detection shell, and the other part of the bearing substrate is positioned at the shell head part of the detection shell; the water absorption part is adhered to the surface of the bearing substrate and is positioned at the shell part of the detection shell; the collecting part is adhered to the surface of the bearing substrate and is positioned at the shell head part of the detection shell; the detection part is adhered to the bearing substrate between the water absorption part and the acquisition part, and corresponds to the detection window at the fixed position of the bearing substrate;
the collecting part comprises a filter paper strip, and the filter paper strip is adhered to the surface of the bearing substrate; the water absorption part comprises a plurality of layers of water absorption paper strips, the water absorption paper strips are laminated and adhered into a whole, and the adhered water absorption paper strips are connected with the bearing substrate; the detection part comprises a chromatographic membrane, one end of the chromatographic membrane extends into the lower surface of the lowest water absorption strip in the water absorption part, the other end of the chromatographic membrane is connected with a filter paper strip of the acquisition part, a connecting sheet is arranged at the connection position of the chromatographic membrane and the filter paper strip, and the connecting sheet reinforces the connection position of the chromatographic membrane and the filter paper strip; a dust shielding baffle is arranged on the upper surface of at least one layer of the water absorption paper strips, one end of the dust shielding baffle is adhered to the water absorption paper strips, and the other end of the dust shielding baffle extends to the position right above the chromatographic membrane;
the light source assembly is arranged on the detection window of the detection shell, faces the detection part of the test paper assembly, and is used for providing illumination for the detection part.
Further, the light source component adopts LED lamp beads which disperse ultraviolet light, the LED lamp beads are positioned at the narrow side positions of the detection window, and the LED lamp beads are relatively fixed at the narrow side positions of the detection window.
Further, the light source assembly adopts an LED lamp strip which diverges ultraviolet light, the LED lamp strip is arranged at the edge of the detection window, and the LED lamp strip is fixed around the edge of the detection window.
Further, a switch assembly and a power supply assembly are arranged on the first shell, the switch assembly is positioned on the outer surface of the tail part of the first shell, and the switch assembly is used for controlling the opening and closing of the light source assembly; the power supply assembly is located on the inner surface of the tail portion of the first shell, and is electrically connected with the light source assembly and used for providing power for the light source assembly.
Further, the inner surface of the first housing is provided with a wire groove for accommodating the connecting wire, one end of the wire groove extends to the position of the power supply assembly, and the other end of the wire groove extends to the position of the light source assembly.
Further, the inner surface of the first shell is provided with a plurality of pairs of fixing columns along the length direction; the inner surface of the second shell is provided with a plurality of pairs of fixing holes along the length direction; when the first shell and the second shell are connected into a whole, the distribution positions of the fixing columns on the first shell correspond to the distribution positions of the fixing holes on the second shell one by one; each fixing column is connected with the fixing hole at the corresponding position in an interference fit mode.
Further, the collection hole on the first shell is in an inverted cone shape, the opening of the collection hole faces the outer surface of the shell head, and the minimum diameter of the collection hole is smaller than the width of the collection part.
Further, an arc-shaped concave surface is arranged in the first shell corresponding to the shell tail surface, and an arc-shaped concave surface is arranged in the second shell corresponding to the shell tail surface; the arc concave surfaces on the first shell and the second shell jointly form a first holding part of the detection pen, and the first holding part is used for holding the detection pen when collecting the reagent.
Further, a second holding part is arranged at the connecting position of the shell body and the shell tail in the detection shell, and the second holding part is used for holding when observing the detection part of the test paper component in the detection pen.
Further, a plurality of supporting ribs are arranged on the shell part of the second shell, and the supporting ribs are used for supporting and fixing the water absorbing part of the test paper assembly; the shell body part of the first shell is provided with a plurality of compression columns, and the compression columns are used for applying compression force to the water absorption part of the test paper assembly.
Compared with the prior art, the invention has the following advantages:
1) According to the detection pen, the light source with the preset wavelength is arranged near the detection window, the reagent strip with higher sensitivity, better specificity and wider detection linearity is manufactured by adopting the fluorescent material to replace a colloidal gold product, and under the condition that the concentration of the fluorescent material in the detected material is smaller, the fluorescent material with low concentration can be excited to emit fluorescence through the irradiation of the light source, so that the detection pen can be directly observed by human eyes, the detection precision is higher, the detectable concentration range is wider, and the universality is better.
2) The detection pen adopts a detachable connection mode, wherein the first shell and the second shell are connected and fixed into a whole through the interference fit of the fixing column and the fixing hole, when the first shell and the second shell are connected into a whole, the connection positions of the first shell and the second shell are kept sealed, the test paper assembly is fixed in the detection shell, when the test paper assembly needs to be replaced, the first shell and the second shell can be detached, the used test paper assembly is taken out and replaced by a new test paper assembly, so that the detection pen can be reused, and the connection mode of the two shells is simple, and the disassembly and the installation are very convenient.
3) According to the detection pen, the detection shell structure is arranged, so that when the test paper assembly is arranged in the detection shell, the shell head part and the shell body part of the detection shell can be kept separated, and the shell body part of the detection shell is kept in a dry state in the detection process by arranging the drying agent at the shell tail part, so that water molecules evaporated from the water absorbing part in the test paper assembly can be kept by the drying agent, electronic elements in the detection pen are prevented from being corroded by the water molecules, and the service life of the detection pen is prolonged.
Drawings
FIG. 1 is a schematic diagram of a test pen according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view showing the internal structure of a test pen according to an embodiment of the present invention;
FIG. 3 is an exploded view of a test pen in accordance with an embodiment of the present invention;
FIG. 4 is a schematic view of the structure of the first housing according to the embodiment of the present invention;
FIG. 5 is an enlarged schematic view of the position A of FIG. 4;
FIG. 6 is a schematic view of the structure of the second housing according to the embodiment of the present invention;
FIG. 7 is a schematic diagram of a test paper assembly according to an embodiment of the invention.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
Example 1:
as shown in fig. 1-7, in this embodiment, a fluorescence excitation type detection pen is disclosed, including: the test paper assembly comprises a detection shell, a test paper assembly 3, a light source assembly 11 and a pen cap 5.
Specifically, referring to fig. 1, the detection housing is integrally divided into a housing ii, a housing head iii and a housing tail i, wherein the detection housing includes a first housing 1 and a second housing 2, the first housing 1 and the second housing 2 are detachably connected, and an accommodating space is provided between the first housing 1 and the second housing 2; the first housing 1 is provided with a detection window 9 corresponding to the housing ii, the first housing 1 is provided with a collection hole 10 corresponding to the housing iii, it should be noted that the collection hole 10 on the first housing 1 is in an inverted cone shape, the opening of the collection hole 10 faces the outer surface of the housing iii, the minimum diameter of the collection hole 10 is smaller than the width of the collection portion 303, when the sample liquid is instilled on the collection hole 10, the sample liquid can be kept in contact with the collection portion 303 only, the sample liquid is prevented from flowing to the slit inside the housing, the pen cap 5 is partially nested with the housing iii of the detection housing, and the purpose of the pen cap 5 is to keep the collection hole 10 in the detection pen clean.
In detail, referring to fig. 7, the test paper assembly 3 includes a water absorbing portion 301, a detecting portion 302, a collecting portion 303, and a carrying substrate 306, where the carrying substrate 306 is in a strip-shaped plate structure, the carrying substrate 306 is mainly used to provide a carrying platform for other components in the test paper assembly 3, a part of the carrying substrate 306 is located in a shell ii portion of the detecting housing, and another part of the carrying substrate 306 extends to a shell iii portion of the detecting housing; the water absorbing part 301 is adhered to the surface of the bearing substrate 306, the water absorbing part 301 is positioned at the shell II part of the detection shell, and the water absorbing part 301 is used for absorbing the water of the detection part 302 so as to further maintain the osmotic pressure of the sample liquid in the chromatographic permeation process of the detection part 302; the collecting part 303 is adhered to the surface of the bearing substrate 306, the collecting part 303 is positioned at the shell head III part of the detection shell, one end of the collecting part 303 extends to the position of the collecting hole 10, and when the sample liquid is dripped from the collecting hole 10, the sample liquid can be accurately dripped on the collecting part 303; the detection part 302 is adhered to the bearing substrate 306 between the water absorbing part 301 and the collecting part 303, and the detection part 302 corresponds to the detection window 9 at the fixed position of the bearing substrate 306, and the detection part 302 is a main detection part 302 piece in the test paper assembly 3 and is made of a material with good chromatographic performance and high permeability.
In more detail, the collecting portion 303 includes a filter paper strip, and the filter paper strip is adhered to the surface of the carrier substrate 306; the water absorbing part 301 comprises a plurality of layers of water absorbing paper strips, the water absorbing paper strips are laminated and adhered into a whole, and the adhered water absorbing paper strips are connected with the bearing substrate 306; the detection part 302 comprises a chromatographic membrane, one end of the chromatographic membrane extends into the lower surface of the lowest water absorption paper strip in the water absorption part 301, the other end of the chromatographic membrane is connected with a filter paper strip of the collection part 303, a connecting sheet 304 is arranged at the connection position of the chromatographic membrane and the filter paper strip, and the connecting sheet 304 reinforces the connection position of the chromatographic membrane and the filter paper strip; the upper surface of at least one layer of absorbent paper strip is provided with a dust shielding separation blade 305, one end of the dust shielding separation blade 305 is adhered to the absorbent paper strip, and the other end of the dust shielding separation blade 305 extends to the position right above the chromatographic membrane.
The filter paper strip of the collecting part 303 in the test paper assembly 3 can adsorb sample liquid in the detection process, and as the filter paper is made of fiber, the surface of the filter paper is provided with numerous small holes for liquid particles to pass through, and solid particles with larger volume can not pass through, when the filter paper strip in the detection pen stretches into the sample liquid, the filter paper strip can perform preliminary filtration on the sample liquid entering the detection pen collecting hole 10 to remove large-particle impurities; the filtered sample liquid flows onto the chromatographic membrane of the detection part 302 through innumerable small holes in the filter paper, the detected substances in the sample liquid are separated by chromatography on the chromatographic membrane, the residual liquid continuously permeates into the water absorption paper strip of the water absorption part 301, and the detected substances continuously accumulate on the chromatographic membrane by separation through the sample liquid continuously passing through the filter paper strip, the chromatographic membrane and the water absorption paper strip. The dust shielding separation blade 305 arranged above the chromatographic membrane can keep the chromatographic membrane clean, and prevent external dust from entering from the detection window 9 to pollute the chromatographic membrane and influence the detection result.
Further, as shown in fig. 4 and 5, the light source assembly 11 is mounted on the detection window 9 of the detection housing, the light source assembly 11 faces the detection portion 302 of the test paper assembly 3, and the light source assembly 11 is used for providing illumination to the detection portion 302. In more detail, the light source assembly 11 adopts an LED lamp bead that diverges ultraviolet light, the LED lamp bead is located at the narrow side position of the detection window 9, and the LED lamp bead is relatively fixed at the narrow side position of the detection window 9. Or alternatively, the light source assembly 11 also adopts an LED lamp strip for dispersing ultraviolet light, the LED lamp strip is arranged at the edge of the detection window 9, and the LED lamp strip is fixed around the edge of the detection window 9
The light source component 11 is a light source with a specific wavelength, and the wavelength of the light source in this embodiment is preferably 365-395 nm, and the light source is mainly used for providing illumination to the detection portion 302, and the detected substance in the sample solution is irradiated by the ultraviolet light source under the condition of low concentration, so as to excite the color of the detected substance in the detection portion 302, and the ultraviolet light can excite the fluorescent reagent with quantum dots of the detection portion on the test paper component, so that the detection result is clearly displayed, further ensuring that the detection pen can still accurately detect under the condition of low concentration, improving the detectable range of the detection pen, and improving the accuracy of the detection result.
In more detail, the first housing 1 is provided with a switch assembly 8 and a power supply assembly 4, the power supply assembly 4 is located at a housing tail i portion of the detection housing, the power supply assembly 4 is located at an inner surface of the first housing tail i portion, the power supply assembly 4 is electrically connected with the light source assembly 11, the power supply assembly 4 is used for providing specific power for the light source assembly 11, the housing tail portion of the first housing 1 is provided with a battery fixing portion 102, the battery fixing portion 102 is provided with an electrode plate, and the second housing is also provided with a fixing portion 203 which is against the battery; the power supply assembly 4 is electrically connected with the light source assembly 11; the switch 8 is located at the tail I part of the detection shell, and the switch component 8 is used for controlling the on-off of the light source component 11. The light control component 8, the power supply component 4 and the light source component 11 can be conducted through connecting wires. Further, the inner surface of the first housing 1 is provided with a wire groove 104 for accommodating the connecting wire, one end of the wire groove 104 extends to the position of the power supply assembly 4, and the other end of the wire groove 104 extends to the position of the light source assembly 11.
Further, in the detection pen, the first housing 1 and the second housing 2 are detachable, specifically, as shown in fig. 4 and 6, the inner surface of the first housing 1 is provided with a plurality of pairs of fixing posts 101 along the length direction; the inner surface of the second housing 2 is provided with a plurality of pairs of fixing holes 201 along the length direction; when the first casing 1 and the second casing 2 are connected into a whole, the distribution positions of the fixing columns 101 on the first casing 1 are in one-to-one correspondence with the distribution positions of the fixing holes 201 on the second casing 2; each fixing column 101 is connected with the fixing hole 201 at the corresponding position in an interference fit manner. Through above-mentioned fixed knot constructs, can make the detection casing conveniently assemble and dismantle, the test paper subassembly 3 of later stage of being convenient for change, reuse detects the pen.
In addition, two structures for holding are arranged in the detection pen, specifically, as shown in fig. 3, an arc concave surface is arranged on the surface of the first shell 1 corresponding to the shell tail i, and an arc concave surface is arranged on the surface of the second shell 2 corresponding to the shell tail i; the arc concave surfaces on the first shell 1 and the second shell 2 jointly form a first holding part 6 of the detection pen, and the first holding part 6 is used for holding the detection pen when collecting the reagent. And the connection position of the shell II and the shell tail I in the detection shell is provided with a second holding part 7, and the second holding part 7 is used for holding when observing the detection part 302 of the test paper component 3 in the detection pen. The two holding parts can be changed according to different occasions in the use process of the detection pen, for example, when the sample liquid is collected, the first holding part 6 can be held, so that the detection pen stretches into a container containing the sample liquid, and when the detection window 9 of the detection pen is observed, the second holding part 7 is held, and the detection window 9 is kept perpendicular to the observation line.
Further, as shown in fig. 4 and 6, the casing ii of the second casing 2 is provided with a plurality of supporting ribs 203, and the supporting ribs 203 are used for supporting and fixing the water absorbing portion 301 of the test paper assembly 3; the shell II part of the first shell 1 is provided with a plurality of compression columns 103, the compression columns 103 are used for applying compression force to the water absorbing part 301 of the test paper assembly 3, and the support ribs 203 and the compression columns 103 can fix the water absorbing part 301 of the test paper assembly 3 to prevent the test paper assembly 3 from shaking.
In addition, the tail I part of the detection shell is provided with an annular fixing protrusion (not shown), and specifically, the tail I part of the first shell 1 is provided with a fixing protrusion I, the tail I part of the second shell 2 is provided with a fixing protrusion II, and the fixing protrusion I and the fixing protrusion II form a complete fixing protrusion for accommodating a drying agent. The drying agent is used for absorbing moisture contained in air of the shell body and the shell tail part in the detection shell, and keeping the inside of the detection shell dry.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention are included in the protection scope of the present invention.
Claims (6)
1. A fluorescence excitation type detection pen, characterized by comprising:
the detection shell is integrally divided into a shell body, a shell head and a shell tail, wherein the detection shell comprises a first shell and a second shell, the first shell and the second shell are detachably connected, and an accommodating space is arranged between the first shell and the second shell; the first shell is provided with a detection window corresponding to the shell body, and the first shell is provided with a collection hole corresponding to the shell head;
the test paper assembly comprises a water absorption part, a detection part, a collection part and a bearing substrate, wherein the bearing substrate is of a strip-shaped plate structure, one part of the bearing substrate is positioned at the shell part of the detection shell, and the other part of the bearing substrate is positioned at the shell head part of the detection shell; the water absorption part is adhered to the surface of the bearing substrate and is positioned at the shell part of the detection shell; the collecting part is adhered to the surface of the bearing substrate and is positioned at the shell head part of the detection shell; the detection part is adhered to the bearing substrate between the water absorption part and the acquisition part, and corresponds to the detection window at the fixed position of the bearing substrate;
the collecting part comprises a filter paper strip, and the filter paper strip is adhered to the surface of the bearing substrate; the water absorption part comprises a plurality of layers of water absorption paper strips, the water absorption paper strips are laminated and adhered into a whole, and the adhered water absorption paper strips are connected with the bearing substrate; the detection part comprises a chromatographic membrane, one end of the chromatographic membrane extends into the lower surface of the lowest water absorption strip in the water absorption part, the other end of the chromatographic membrane is connected with a filter paper strip of the acquisition part, a connecting sheet is arranged at the connection position of the chromatographic membrane and the filter paper strip, and the connecting sheet reinforces the connection position of the chromatographic membrane and the filter paper strip; a dust shielding baffle is arranged on the upper surface of at least one layer of the water absorption paper strips, one end of the dust shielding baffle is adhered to the water absorption paper strips, and the other end of the dust shielding baffle extends to the position right above the chromatographic membrane;
the light source assembly is arranged on the detection window of the detection shell, faces the detection part of the test paper assembly and is used for providing illumination for the detection part;
the first shell is provided with a switch assembly and a power supply assembly, the switch assembly is positioned on the outer surface of the tail part of the first shell, and the switch assembly is used for controlling the opening and closing of the light source assembly; the power supply assembly is positioned on the inner surface of the tail part of the first shell, is electrically connected with the light source assembly and is used for providing power for the light source assembly;
the LED lamp strip or the LED lamp beads which emit ultraviolet light are arranged at the edge of the detection window, the LED lamp strip is fixed around the edge of the detection window, the LED lamp beads are positioned at the narrow edge of the detection window, and the LED lamp beads are relatively fixed at the narrow edge of the detection window;
the shell part of the second shell is provided with a plurality of supporting ribs, and the supporting ribs are used for supporting and fixing the water absorbing part of the test paper assembly; the shell part of the first shell is provided with a plurality of compression columns which are used for applying compression force to the water absorbing part of the test paper assembly;
and a drying agent is arranged in the shell tail.
2. The test pen of claim 1, wherein: the internal surface of first casing is equipped with the metallic channel that is used for holding connecting wire, the one end of metallic channel extends to power supply subassembly position, and the other end of metallic channel extends to light source subassembly position.
3. The test pen of claim 1, wherein: the inner surface of the first shell is provided with a plurality of pairs of fixing columns along the length direction; the inner surface of the second shell is provided with a plurality of pairs of fixing holes along the length direction; when the first shell and the second shell are connected into a whole, the distribution positions of the fixing columns on the first shell correspond to the distribution positions of the fixing holes on the second shell one by one; each fixing column is connected with the fixing hole at the corresponding position in an interference fit mode.
4. The test pen of claim 1, wherein: the collection hole on the first casing is the back taper, just the opening of collection hole is towards the surface of shell head, the minimum diameter of collection hole is less than the width of collection portion.
5. The test pen of claim 1, wherein: an arc-shaped concave surface is arranged in the first shell corresponding to the shell tail surface, and an arc-shaped concave surface is arranged on the shell tail surface in the second shell; the arc concave surfaces on the first shell and the second shell jointly form a first holding part of the detection pen, and the first holding part is used for holding the detection pen when collecting the reagent.
6. The test pen of claim 1, wherein: the connecting position of the shell body and the shell tail in the detection shell is provided with a second holding part, and the second holding part is used for holding when observing the detection part of the test paper component in the detection pen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110653463.8A CN113406048B (en) | 2021-06-11 | 2021-06-11 | Fluorescence excitation type detection pen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110653463.8A CN113406048B (en) | 2021-06-11 | 2021-06-11 | Fluorescence excitation type detection pen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113406048A CN113406048A (en) | 2021-09-17 |
| CN113406048B true CN113406048B (en) | 2023-04-25 |
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| JP3738357B2 (en) * | 2002-08-05 | 2006-01-25 | 株式会社創成電子 | Portable sample analyzer |
| CN1645146A (en) * | 2005-02-03 | 2005-07-27 | 厦门大学 | Immune chromatography with fluorescent rare earth nanometer particle as marker and detecting testing paper strip |
| CN1866012A (en) * | 2006-06-02 | 2006-11-22 | 上海新波生物技术有限公司 | Quantitative and quick immune detection method and special apparatus therefor |
| JP4360652B2 (en) * | 2007-06-08 | 2009-11-11 | 古河電気工業株式会社 | Labeled silica nanoparticles for immunochromatography reagent, immunochromatography reagent, test strip for immunochromatography using the same, and fluorescence detection system for immunochromatography |
| CN101493460B (en) * | 2009-02-25 | 2012-06-27 | 江西中德生物工程有限公司 | Method for producing fluorescent microballoons immune chromatography test paper stripe and quantitative determination method |
| JP5367490B2 (en) * | 2009-07-29 | 2013-12-11 | 古河電気工業株式会社 | Test strip for immunochromatography |
| CN102023210A (en) * | 2009-09-21 | 2011-04-20 | 北京中德大地食品安全技术开发有限责任公司 | Fluorescent microsphere immunochromatography detection card for detecting enrofloxacin and preparation method thereof |
| CN103197074B (en) * | 2013-04-22 | 2015-11-25 | 北京润博福得生物科技发展有限公司 | Based on the immunochromatography quantitative detecting reagent of near-infrared fluorescent Nano microsphere label |
| CN108254562B (en) * | 2016-12-28 | 2019-11-08 | 广州瑞博奥生物科技有限公司 | Detect time-resolved fluoroimmunoassay chromatograph test strip, the kit and preparation method thereof of MYO |
| CN207423976U (en) * | 2017-05-02 | 2018-05-29 | 网易（杭州）网络有限公司 | Test strips and detection device |
| CN108802407A (en) * | 2018-05-29 | 2018-11-13 | 郑州左安检测科技有限公司 | A kind of test strips and its preparation method and application method of detection morphine |
| CN110275017A (en) * | 2019-07-25 | 2019-09-24 | 烟台简单生物技术有限公司 | A kind of one-stop detection pen of fluorescence and detector bar |
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