CN113406048A - Fluorescence excitation type detection pen - Google Patents
Fluorescence excitation type detection pen Download PDFInfo
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- CN113406048A CN113406048A CN202110653463.8A CN202110653463A CN113406048A CN 113406048 A CN113406048 A CN 113406048A CN 202110653463 A CN202110653463 A CN 202110653463A CN 113406048 A CN113406048 A CN 113406048A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- General Health & Medical Sciences (AREA)
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- General Physics & Mathematics (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to a fluorescence excitation type detection pen, which is characterized in that a light source with a preset wavelength is arranged near a detection window in the detection pen, a reagent strip with higher sensitivity, better specificity and wider detection linearity is made of a fluorescent material to replace a colloidal gold product, and a low-concentration fluorescent material can be excited to emit fluorescence by the irradiation of the light source under the condition that the concentration of the fluorescent material in a detected material is smaller, so that human eyes can directly observe the fluorescent material, the detection pen has higher detection precision, the detectable concentration range is wider, and the universality is better.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and relates to a fluorescence excitation type detection pen.
Background
The fluorescent substance on the test paper of the traditional detection pen must reach a certain concentration to be visible to the naked eye. Therefore, the analyte needs to reach a certain concentration to cause the fluorescent substance to react and generate fluorescence. At present, a detection pen or detection test paper on the market mainly adopts a colloidal gold method as a main part, when the detection pen or the detection test paper is used in occasions such as the early pregnancy, once the HCG concentration is lower than a certain degree, the result cannot be accurately measured, and the anti-interference capability of colloidal gold is inferior to that of other fluorescent materials, such as common fluorescent microspheres and quantum dot fluorescent microspheres.
The reagent product adopting the colloidal gold method mostly performs result judgment by visually observing the color with naked eyes, the identification and judgment of the color have subjectivity to a certain degree, particularly, the result judgment near a critical value can cause interpretation trouble for a client, and the fluorescent reagent has higher sensitivity, has higher sensitivity compared with the current colloidal gold product, judges a detection result near the critical value, has more advantages and better reflected signal, replaces color identification with fluorescence, and is easier to perform interpretation for the naked eye identification.
Chinese patent document CN107356767A discloses a test strip analyzer for luteinizing hormone, which includes a reagent card and a test pen; the detection pen comprises a shell, a shading cavity arranged in the shell and a photoelectric detection system used for judging the color depth, wherein an insertion opening is formed in the shell of the detection pen, and when a reagent card is inserted into the detection pen through the insertion opening, a detection window is correspondingly inserted into the shading cavity. It gathers the reflection light of test paper strip colour shade change in-process through photoelectric detection system, and convert light signal into digital signal of telecommunication, obtain the testing result according to threshold value comparison automatic judgement, thereby improve the precision and the measurement of measuring result, and non-contact detection has been realized, avoid the pollution to the test pen, and simultaneously, through with reagent card and the design of test pen components of a whole that can function independently, make the test pen can recycle, detection cost has been practiced thrift, but the test card that this structure adopted discerns through contrast colour change and judges the testing result, it passes through the reflection light of photoelectric detection system identification test card colour shade change in-process, and then contrast and obtain the result, this structure easily receives external environment light influence, interference killing feature is relatively weak.
Chinese patent document CN102033129A discloses a test pen for detecting pathogenic microorganisms, which comprises blood filtering paper, a colloidal gold pad, a nitrocellulose membrane, absorbent filter paper and a PVC base plate; the colloidal gold pad is coated with a colloidal gold compound which is a mouse anti-human INF-gamma monoclonal antibody, the nitrocellulose membrane is provided with a test line and a control line, the test line is coated with IFN-gamma, the control line is coated with goat anti-mouse IgG polyclonal antibody, after a sample is dripped to a water absorption rod, the IFN-gamma in the sample moves to the colloidal gold pad along with the solution and is combined with the gold-labeled IFN-gamma monoclonal antibody to form an antigen-antibody compound, if the IFN-gamma in the sample is not enough, the gold-labeled IFN-gamma monoclonal antibody can be combined with the antigen IFN-gamma again, and the monoclonal antibodies can be combined with the IFN-gamma at the T line when moving to the T line along with the solution to make the T line red; conversely, if there is an excess of IFN- γ in the sample such that the gold-labeled IFN- γ monoclonal antibodies can no longer bind to the antigen IFN- γ, these monoclonal antibodies will not bind to IFN- γ at the T-line as the solution moves to the T-line, and no color will appear in the T-line. The gold-labeled IFN-gamma monoclonal antibody which is not combined with the T line continuously moves to the C line along with the solution, and is combined with the goat anti-mouse polyclonal antibody at the C line to make the C line red, so that the mouse anti-human IFN-gamma monoclonal antibody can be identified by the goat anti-mouse polyclonal antibody, but a nitrocellulose membrane adopted in the structure is also identified by judging color change to obtain a detection result, the nitrocellulose membrane has requirements on the concentration of a detected object in a sample, and once the concentration of the detected object is too low, the change color of the nitrocellulose membrane is difficult to visually observe by naked eyes.
Disclosure of Invention
Aiming at the problems in the prior art, the invention discloses a fluorescence excitation type detection pen, which is characterized in that a light source with a preset wavelength is arranged near a detection window in the detection pen, a fluorescent material is selected to manufacture a reagent strip with higher sensitivity, better specificity and wider detection linearity to replace a colloidal gold product, and a low-concentration fluorescent material can be excited to emit fluorescence by the irradiation of the light source under the condition that the concentration of the fluorescent material in a detected material is smaller, so that human eyes can directly observe the fluorescent material, the detection pen has higher detection precision, wider detectable concentration range and better universality.
The technical scheme adopted by the invention for solving the technical problems is as follows: a fluorescence excitation-type test pen, comprising:
the detection shell is integrally divided into a shell body, a shell head and a shell tail, wherein the detection shell comprises a first shell and a second shell which are detachably connected, and an accommodating space is arranged between the first shell and the second shell; the first shell is provided with a detection window corresponding to the shell body, and the first shell is provided with a collection hole corresponding to the shell head;
the test paper assembly comprises a water absorbing part, a detecting part, a collecting part and a bearing substrate, wherein the bearing substrate is of a strip plate structure, one part of the bearing substrate is positioned on the shell body part of the detecting shell, and the other part of the bearing substrate is positioned on the shell head part of the detecting shell; the water absorption part is adhered to the surface of the bearing substrate and is positioned on the shell body part of the detection shell; the acquisition part is adhered to the surface of the bearing substrate and is positioned on the shell head part of the detection shell; the detection part is bonded on the bearing substrate between the water absorption part and the acquisition part and corresponds to the detection window at the fixed position of the bearing substrate;
the collecting part comprises a filter paper strip which is adhered to the surface of the bearing substrate; the water absorption part comprises a plurality of layers of water absorption paper strips, the water absorption paper strips are laminated and adhered into a whole, and the water absorption paper strips adhered into a whole are connected with the bearing substrate; the detection part comprises a chromatographic membrane, one end of the chromatographic membrane extends into the lower surface of the water absorption part, which is positioned at the bottommost water absorption paper strip, the other end of the chromatographic membrane is connected with the filter paper strip of the acquisition part, and a connecting sheet is arranged at the connecting position of the chromatographic membrane and the filter paper strip and used for reinforcing the connecting part of the chromatographic membrane and the filter paper strip; a dust shielding baffle is arranged on the upper surface of at least one layer of water-absorbing paper strips, one end of the dust shielding baffle is bonded with the water-absorbing paper strips, and the other end of the dust shielding baffle extends to the position right above the chromatographic membrane;
the light source assembly is arranged on the detection window of the detection shell, faces to the detection part of the test paper assembly and is used for providing illumination for the detection part.
Further, the light source component adopts LED lamp beads for dispersing ultraviolet light, the LED lamp beads are located at the narrow side positions of the detection windows, and the LED lamp beads are relatively fixed at the narrow side positions of the detection windows.
Further, the light source assembly adopts an LED lamp strip for emitting ultraviolet light, the LED lamp strip is arranged at the edge of the detection window, and the LED lamp strip is fixed around the edge of the detection window.
Furthermore, a switch assembly and a power supply assembly are arranged on the first shell, the switch assembly is positioned on the outer surface of the tail part of the first shell, and the switch assembly is used for controlling the on-off of the light source assembly; the power supply assembly is located on the inner surface of the tail part of the first shell and electrically connected with the light source assembly, and the power supply assembly is used for supplying electric power to the light source assembly.
Furthermore, the inner surface of the first shell is provided with a wire groove for accommodating a connecting wire, one end of the wire groove extends to the position of the power supply assembly, and the other end of the wire groove extends to the position of the light source assembly.
Furthermore, a plurality of pairs of fixing columns are arranged on the inner surface of the first shell along the length direction; the inner surface of the second shell is provided with a plurality of pairs of fixing holes along the length direction; when the first shell and the second shell are connected into a whole, the distribution positions of the fixing columns on the first shell correspond to the distribution positions of the fixing holes on the second shell one by one; and each fixing column is connected with the fixing hole at the corresponding position in an interference fit mode.
Further, gather the hole on the first casing and be the back taper, just gather the opening in hole and towards the surface of shell head, the minimum diameter of gathering the hole is less than the width of gathering the portion.
Further, an arc-shaped concave surface is arranged on the surface, corresponding to the shell tail, of the first shell, and an arc-shaped concave surface is arranged on the surface, corresponding to the shell tail, of the second shell; arc concave surface on first casing and the second casing constitutes the first portion of holding of detecting the pen jointly, first portion of holding is used for detecting to hold when pen gathers reagent and holds.
Furthermore, the connecting position of the shell body and the shell tail in the detection shell is provided with a second holding part, and the second holding part is used for holding when observing the detection part of the test paper component in the detection pen.
Furthermore, a plurality of support ribs are arranged on the shell body part of the second shell and used for supporting and fixing the water absorption part of the test paper component; the shell body part of the first shell is provided with a plurality of compression columns, and the compression columns are used for applying compression force to the water absorption part of the test paper assembly.
Compared with the prior art, the invention has the following advantages:
1) according to the detection pen, the light source with the preset wavelength is arranged near the detection window, the reagent strip with higher sensitivity, better specificity and wider detection linearity is made of the fluorescent material to replace a colloidal gold product, and the fluorescent material with low concentration can be excited to emit fluorescence by irradiation of the light source under the condition that the concentration of the fluorescent material in the detected substance is smaller, so that human eyes can directly observe the reagent strip, the detection accuracy of the detection pen is higher, the detectable concentration range is wider, and the universality is better.
2) The test pen adopts a detachable connection mode, wherein the first shell and the second shell are fixedly connected with the fixing hole into a whole through the fixed column in an interference fit manner, when the first shell and the second shell are connected into a whole, the connection position of the first shell and the second shell is kept sealed, the test paper component is fixed in the test shell, and when the test paper component needs to be replaced, the used test paper component can be taken out and replaced by a new test paper component after the first shell and the second shell are detached, so that the test pen can be repeatedly utilized.
3) According to the detection pen, the detection shell structure is arranged, so that when the test paper assembly is arranged in the detection shell, the shell head part and the shell body part of the detection shell can be kept separated, the drying agent is arranged at the shell tail part, the shell body part of the detection shell is kept in a dry state in the detection process, water molecules evaporated from a water absorption part in the test paper assembly can be kept by the drying agent, the water molecules are prevented from corroding electronic elements in the detection pen, and the service life of the detection pen is prolonged.
Drawings
FIG. 1 is a schematic structural diagram of a test pen according to an embodiment of the present invention;
FIG. 2 is a sectional view showing the internal structure of the test pen in the embodiment of the present invention;
FIG. 3 is an exploded view of a test pen in an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a first housing in an embodiment of the invention;
FIG. 5 is an enlarged schematic view of position A of FIG. 4;
FIG. 6 is a schematic structural view of a second housing in an embodiment of the invention;
FIG. 7 is a schematic diagram of the structure of a test strip assembly according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Example 1:
as shown in fig. 1 to 7, the present embodiment discloses a fluorescence excitation type detection pen, which includes: the test paper comprises a detection shell, a test paper component 3, a light source component 11 and a pen cap 5.
Specifically, as shown in fig. 1, the detection shell is integrally divided into a shell body ii, a shell head iii and a shell tail i, wherein the detection shell includes a first shell 1 and a second shell 2, the first shell 1 and the second shell 2 are detachably connected, and an accommodating space is arranged between the first shell 1 and the second shell 2; wherein it is equipped with detection window 9 to correspond II positions of shell body in the first casing 1, it is equipped with collection hole 10 to correspond III positions of shell head in the first casing 1, must notice that, collection hole 10 on the first casing 1 is the back taper, just the opening of gathering hole 10 is towards the surface of shell head III, the minimum diameter of gathering hole 10 is less than the width of collection portion 303, when sample liquid instills on gathering hole 10, can keep sample liquid only to contact with collection portion 303, avoids sample liquid to flow to the inside gap of casing, cap 5 then is nested with the shell head III part of detecting the casing, cap 5's purpose is for keeping collection hole 10 clean in the detection pen.
In detail, referring to fig. 7, the test strip assembly 3 includes a water absorbing portion 301, a detecting portion 302, a collecting portion 303 and a carrying substrate 306, wherein the carrying substrate 306 is a strip-shaped plate structure, the carrying substrate 306 is mainly used for providing a carrying platform for other components in the test strip assembly 3, a part of the carrying substrate 306 is located at a housing ii portion of the detecting housing, and the other part of the carrying substrate 306 extends to a housing head iii portion of the detecting housing; the water absorbing part 301 is adhered to the surface of the bearing substrate 306, the water absorbing part 301 is positioned at the shell body II of the detection shell, and the water absorbing part 301 is used for absorbing water of the detection part 302 so as to keep the osmotic pressure of the sample liquid in the chromatography and osmosis process of the detection part 302; the collecting part 303 is adhered to the surface of the bearing substrate 306, the collecting part 303 is positioned at the shell head III part of the detection shell, one end of the collecting part 303 extends to the position of the collecting hole 10, and when sample liquid is dripped from the collecting hole 10, the sample liquid can be accurately dripped on the collecting part 303; the detection part 302 is adhered to the bearing substrate 306 between the water absorption part 301 and the collection part 303, the detection part 302 corresponds to the detection window 9 at the fixed position of the bearing substrate 306, and the detection part 302 is a main detection part 302 in the test paper component 3 and is made of a material with good chromatographic performance and high permeability.
In more detail, the collection portion 303 includes a filter paper strip, and the filter paper strip is adhered to the surface of the carrier substrate 306; the water absorption part 301 comprises a plurality of layers of water absorption paper strips, the water absorption paper strips are laminated and adhered into a whole, and the water absorption paper strips adhered into a whole are connected with the bearing substrate 306; the detection part 302 comprises a chromatographic membrane, one end of the chromatographic membrane extends into the lower surface of the water absorption part 301 which is positioned at the bottommost layer of the water absorption paper strip, the other end of the chromatographic membrane is connected with the filter paper strip of the acquisition part 303, a connecting sheet 304 is arranged at the connecting position of the chromatographic membrane and the filter paper strip, and the connecting sheet 304 is used for reinforcing the connecting position of the chromatographic membrane and the filter paper strip; be equipped with on the upper surface of at least one deck absorbent note and hide dirt separation blade 305, the one end and the absorbent note bonding of dirt separation blade 305 hide, the other end that hides dirt separation blade 305 extends to directly over the chromatographic membrane.
The filter paper strip of the collecting part 303 in the test paper assembly 3 can adsorb sample liquid in the detection process, because the filter paper is mostly made of fibers, countless small holes are formed in the surface of the filter paper and can be used for liquid particles to pass through, and solid particles with larger volume cannot pass through, when the filter paper strip in the detection pen extends into the sample liquid, the filter paper strip can preliminarily filter the sample liquid entering the collecting hole 10 of the detection pen, and large-particle impurities are removed; the filtered sample liquid flows to the chromatographic membrane of the detection part 302 through countless small holes in the filter paper, the detected substances in the sample liquid are chromatographically separated on the chromatographic membrane, the residual liquid continuously permeates into the water absorption paper strips of the water absorption part 301, and the detected substances are continuously separated and accumulated on the chromatographic membrane through the sample liquid continuously passing through the filter paper strips, the chromatographic membrane and the water absorption paper strips. The dust shielding baffle 305 arranged above the chromatographic membrane can keep the chromatographic membrane clean, and prevent external dust from entering the chromatographic membrane through the detection window 9 to pollute the chromatographic membrane and influence the detection result.
Further, as shown in fig. 4 and 5, the light source assembly 11 is mounted on the detection window 9 of the detection housing, the light source assembly 11 faces the detection portion 302 of the test strip assembly 3, and the light source assembly 11 is used for providing light to the detection portion 302. More in detail, light source subassembly 11 adopts the LED lamp pearl of dispersing the ultraviolet light, LED lamp pearl is located the narrow limit position of detecting window 9, just LED lamp pearl relatively fixed in the narrow limit position of detecting window 9. Or alternatively, the light source assembly 11 is also provided with an LED strip for emitting ultraviolet light, the LED strip is arranged at the edge of the detection window 9, and the LED strip is fixed around the edge of the detection window 9
The light source assembly 11 is a light source with a specific wavelength, in this embodiment, the wavelength of the light source is preferably 365-.
More specifically, the first casing 1 is provided with a switch assembly 8 and a power supply assembly 4, the power supply assembly 4 is located at a casing tail i part of the detection casing, the power supply assembly 4 is located on an inner surface of the casing tail i part of the first casing, the power supply assembly 4 is electrically connected with the light source assembly 11, the power supply assembly 4 is specifically used for supplying electric power to the light source assembly 11, the casing tail part of the first casing 1 is provided with a battery fixing part 102, an electrode plate is arranged in the battery fixing part 102, and a fixing part 203 which is abutted against the battery is also arranged in the second casing; the power supply assembly 4 is electrically connected with the light source assembly 11; switch 8 is then located the shell tail I part of detecting the casing, switch module 8 is used for controlling the switching of light source subassembly 11. The light control assembly 8, the power supply assembly 4 and the light source assembly 11 can be conducted through connecting wires. Further, the inner surface of the first housing 1 is provided with a wire groove 104 for accommodating a connecting wire, one end of the wire groove 104 extends to the position of the power supply assembly 4, and the other end of the wire groove 104 extends to the position of the light source assembly 11.
Further, the first casing 1 and the second casing 2 of the detection pen are detachable, and specifically, as shown in fig. 4 and 6, a plurality of pairs of fixing posts 101 are arranged on the inner surface of the first casing 1 along the length direction; a plurality of pairs of fixing holes 201 are formed in the inner surface of the second shell 2 along the length direction; when the first shell 1 and the second shell 2 are connected into a whole, the distribution positions of the fixing columns 101 on the first shell 1 correspond to the distribution positions of the fixing holes 201 on the second shell 2 one by one; each fixing column 101 is connected with the fixing hole 201 at the corresponding position in an interference fit mode. Through above-mentioned fixed knot constructs, can make the detection casing conveniently assemble and dismantle, the later stage of being convenient for is changed test paper subassembly 3, and reuse detects the pen.
In addition, the detection pen is provided with two holding structures, specifically, as shown in fig. 3, an arc-shaped concave surface is arranged on the surface of the first shell 1 corresponding to the shell tail i, and an arc-shaped concave surface is arranged on the surface of the shell tail i in the second shell 2; arc concave surface on first casing 1 and the second casing 2 constitutes the first portion 6 of holding of examining the pen jointly, first portion 6 of holding is held when being used for examining the pen and gathering reagent. And the connected position of casing II and casing tail I in the detection casing is equipped with the second and holds portion 7 of taking, the second is held and is held when taking 7 and be arranged in observing the detection portion 302 of test paper subassembly 3 in the detection pen. The two holding parts can be changed according to different occasions in the using process of the detection pen, for example, when sample liquid is collected, the first holding part 6 can be held to enable the detection pen to extend into a container containing the sample liquid, and when a detection window 9 of the detection pen is observed, the second holding part 7 can be held to keep the detection window 9 vertical to an observation sight line.
Further, as shown in fig. 4 and 6, a plurality of support ribs 203 are disposed on the casing ii of the second housing 2, and the support ribs 203 are used for supporting and fixing the water absorbing portion 301 of the test paper assembly 3; the second part of the shell body of the first shell 1 is provided with a plurality of pressing columns 103, the pressing columns 103 are used for applying pressing force to the water absorbing part 301 of the test paper assembly 3, and the water absorbing part 301 of the test paper assembly 3 can be fixed by the support ribs 203 and the pressing columns 103, so that the test paper assembly 3 is prevented from shaking.
In addition, I part of the shell tail of the detection shell is provided with a fixing protrusion (not shown) in an annular shape, specifically, the I part of the shell tail in the first shell 1 is provided with a first fixing protrusion, the I part of the shell tail in the second shell 2 is provided with a second fixing protrusion, and the first fixing protrusion and the second fixing protrusion form a complete fixing protrusion for accommodating a drying agent. The drying agent is used for absorbing moisture contained in air at the shell body and the shell tail part in the detection shell and keeping the interior of the detection shell dry, and because the interior of the detection shell is provided with the electronic element, the drying agent is easy to be corroded by water molecules to cause corrosion short circuit.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.
Claims (10)
1. A fluorescence excitation type detection pen, comprising:
the detection shell is integrally divided into a shell body, a shell head and a shell tail, wherein the detection shell comprises a first shell and a second shell which are detachably connected, and an accommodating space is arranged between the first shell and the second shell; the first shell is provided with a detection window corresponding to the shell body, and the first shell is provided with a collection hole corresponding to the shell head;
the test paper assembly comprises a water absorbing part, a detecting part, a collecting part and a bearing substrate, wherein the bearing substrate is of a strip plate structure, one part of the bearing substrate is positioned on the shell body part of the detecting shell, and the other part of the bearing substrate is positioned on the shell head part of the detecting shell; the water absorption part is adhered to the surface of the bearing substrate and is positioned on the shell body part of the detection shell; the acquisition part is adhered to the surface of the bearing substrate and is positioned on the shell head part of the detection shell; the detection part is bonded on the bearing substrate between the water absorption part and the acquisition part and corresponds to the detection window at the fixed position of the bearing substrate;
the collecting part comprises a filter paper strip which is adhered to the surface of the bearing substrate; the water absorption part comprises a plurality of layers of water absorption paper strips, the water absorption paper strips are laminated and adhered into a whole, and the water absorption paper strips adhered into a whole are connected with the bearing substrate; the detection part comprises a chromatographic membrane, one end of the chromatographic membrane extends into the lower surface of the water absorption part, which is positioned at the bottommost water absorption paper strip, the other end of the chromatographic membrane is connected with the filter paper strip of the acquisition part, and a connecting sheet is arranged at the connecting position of the chromatographic membrane and the filter paper strip and used for reinforcing the connecting part of the chromatographic membrane and the filter paper strip; a dust shielding baffle is arranged on the upper surface of at least one layer of water-absorbing paper strips, one end of the dust shielding baffle is bonded with the water-absorbing paper strips, and the other end of the dust shielding baffle extends to the position right above the chromatographic membrane;
the light source assembly is arranged on the detection window of the detection shell, faces to the detection part of the test paper assembly and is used for providing illumination for the detection part.
2. The test pen according to claim 1, wherein: the light source assembly adopts LED lamp beads for dispersing ultraviolet light, the LED lamp beads are located at the narrow side positions of the detection windows, and the LED lamp beads are relatively fixed at the narrow side positions of the detection windows.
3. The test pen according to claim 1, wherein: the light source component adopts an LED lamp strip which diffuses ultraviolet light, the LED lamp strip is arranged at the edge of the detection window, and the LED lamp strip is fixed around the edge of the detection window.
4. The test pen according to claim 1, wherein: the first shell is provided with a switch assembly and a power supply assembly, the switch assembly is positioned on the outer surface of the tail part of the first shell, and the switch assembly is used for controlling the on-off of the light source assembly; the power supply assembly is located on the inner surface of the tail part of the first shell and electrically connected with the light source assembly, and the power supply assembly is used for supplying electric power to the light source assembly.
5. The test pen according to claim 4, wherein: the inner surface of the first shell is provided with a wire groove for accommodating a connecting wire, one end of the wire groove extends to the position of the power supply assembly, and the other end of the wire groove extends to the position of the light source assembly.
6. The test pen according to claim 1, wherein: the inner surface of the first shell is provided with a plurality of pairs of fixing columns along the length direction; the inner surface of the second shell is provided with a plurality of pairs of fixing holes along the length direction; when the first shell and the second shell are connected into a whole, the distribution positions of the fixing columns on the first shell correspond to the distribution positions of the fixing holes on the second shell one by one; and each fixing column is connected with the fixing hole at the corresponding position in an interference fit mode.
7. The test pen according to claim 1, wherein: the collection hole on the first casing is the back taper, just the opening of gathering the hole is towards the surface of shell head, the minimum diameter of gathering the hole is less than the width of gathering the portion.
8. The test pen according to claim 1, wherein: an arc-shaped concave surface is arranged on the surface of the shell tail in the first shell, and an arc-shaped concave surface is arranged on the surface of the shell tail in the second shell; arc concave surface on first casing and the second casing constitutes the first portion of holding of detecting the pen jointly, first portion of holding is used for detecting to hold when pen gathers reagent and holds.
9. The test pen according to claim 1, wherein: the connection position of shell body and shell tail is equipped with the second and holds the portion of taking in detecting the casing, the second holds the portion of taking and is used for holding when observing the detection portion that detects test paper subassembly in the pen and holds.
10. The test pen according to claim 1, wherein: the shell body part of the second shell is provided with a plurality of supporting ribs, and the supporting ribs are used for supporting and fixing the water absorption part of the test paper component; the shell body part of the first shell is provided with a plurality of compression columns, and the compression columns are used for applying compression force to the water absorption part of the test paper assembly.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004151082A (en) * | 2002-08-05 | 2004-05-27 | Sousei Denshi:Kk | Mobile specimen analyzing apparatus and mobile urine analyzing apparatus |
| CN1645146A (en) * | 2005-02-03 | 2005-07-27 | 厦门大学 | Immune chromatography with fluorescent rare earth nanometer particle as marker and detecting testing paper strip |
| CN1866012A (en) * | 2006-06-02 | 2006-11-22 | 上海新波生物技术有限公司 | Quantitative and quick immune detection method and special apparatus therefor |
| US20090017561A1 (en) * | 2007-06-08 | 2009-01-15 | The Furukawa Electric Co., Ltd. | Labelled silica nanoparticles for immunochromatographic reagent, immunochromatographic reagent, immunochromatographic test strip using the same, and immunochromatographic fluorescence-detecting system or radiation-detecting system |
| CN101493460A (en) * | 2009-02-25 | 2009-07-29 | 江西中德生物工程有限公司 | Method for producing fluorescent microballoons immune chromatography test paper stripe and quantitative determination method |
| JP2011027693A (en) * | 2009-07-29 | 2011-02-10 | Furukawa Electric Co Ltd:The | Test strip for immuno-chromatography |
| CN102023210A (en) * | 2009-09-21 | 2011-04-20 | 北京中德大地食品安全技术开发有限责任公司 | Fluorescent microsphere immunochromatography detection card for detecting enrofloxacin and preparation method thereof |
| CN103197074A (en) * | 2013-04-22 | 2013-07-10 | 北京润博福得生物科技发展有限公司 | Immunochromatography quantitative determination reagent based on near infrared fluorescence nanoparticle markers |
| CN207423976U (en) * | 2017-05-02 | 2018-05-29 | 网易（杭州）网络有限公司 | Test strips and detection device |
| WO2018120855A1 (en) * | 2016-12-28 | 2018-07-05 | 广州瑞博奥生物科技有限公司 | Time-resolved fluorescent immunochromatographic test strip and kit for detecting myo, and preparation method therefor |
| CN108802407A (en) * | 2018-05-29 | 2018-11-13 | 郑州左安检测科技有限公司 | A kind of test strips and its preparation method and application method of detection morphine |
| CN110275017A (en) * | 2019-07-25 | 2019-09-24 | 烟台简单生物技术有限公司 | A kind of one-stop detection pen of fluorescence and detector bar |
-
2021
- 2021-06-11 CN CN202110653463.8A patent/CN113406048B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004151082A (en) * | 2002-08-05 | 2004-05-27 | Sousei Denshi:Kk | Mobile specimen analyzing apparatus and mobile urine analyzing apparatus |
| CN1645146A (en) * | 2005-02-03 | 2005-07-27 | 厦门大学 | Immune chromatography with fluorescent rare earth nanometer particle as marker and detecting testing paper strip |
| CN1866012A (en) * | 2006-06-02 | 2006-11-22 | 上海新波生物技术有限公司 | Quantitative and quick immune detection method and special apparatus therefor |
| US20090017561A1 (en) * | 2007-06-08 | 2009-01-15 | The Furukawa Electric Co., Ltd. | Labelled silica nanoparticles for immunochromatographic reagent, immunochromatographic reagent, immunochromatographic test strip using the same, and immunochromatographic fluorescence-detecting system or radiation-detecting system |
| CN101493460A (en) * | 2009-02-25 | 2009-07-29 | 江西中德生物工程有限公司 | Method for producing fluorescent microballoons immune chromatography test paper stripe and quantitative determination method |
| JP2011027693A (en) * | 2009-07-29 | 2011-02-10 | Furukawa Electric Co Ltd:The | Test strip for immuno-chromatography |
| CN102023210A (en) * | 2009-09-21 | 2011-04-20 | 北京中德大地食品安全技术开发有限责任公司 | Fluorescent microsphere immunochromatography detection card for detecting enrofloxacin and preparation method thereof |
| CN103197074A (en) * | 2013-04-22 | 2013-07-10 | 北京润博福得生物科技发展有限公司 | Immunochromatography quantitative determination reagent based on near infrared fluorescence nanoparticle markers |
| WO2018120855A1 (en) * | 2016-12-28 | 2018-07-05 | 广州瑞博奥生物科技有限公司 | Time-resolved fluorescent immunochromatographic test strip and kit for detecting myo, and preparation method therefor |
| CN207423976U (en) * | 2017-05-02 | 2018-05-29 | 网易（杭州）网络有限公司 | Test strips and detection device |
| CN108802407A (en) * | 2018-05-29 | 2018-11-13 | 郑州左安检测科技有限公司 | A kind of test strips and its preparation method and application method of detection morphine |
| CN110275017A (en) * | 2019-07-25 | 2019-09-24 | 烟台简单生物技术有限公司 | A kind of one-stop detection pen of fluorescence and detector bar |
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|---|---|
| CN113406048B (en) | 2023-04-25 |
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