Cell refrigerating box for long-distance transportation
Technical Field
The invention relates to the field of cell preservation devices, in particular to a cell refrigerating box for long-distance transportation.
Background
Stem cells are a type of pluripotent cells with self-replicating capacity (self-rejuvenating). Under certain conditions, it can differentiate into a variety of functional cells. The stem cells are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells. The stem cells are classified into three categories according to their developmental potential: totipotent Stem Cells (TSC), pluripotent stem cells (pluripotentstem cells) and unipotent stem cells (multipotent stem cells). Stem cells (StemCell) are an insufficiently differentiated, yet immature cell with the potential to regenerate various tissues, organs and the human body, and are known in the medical community as "universal cells".
Current stem cell refrigerated transport case, it itself only plays cold-stored and carry out the effect of the splendid attire transportation of being convenient for to the stem cell thing, but transport incasement portion is not provided with any shock attenuation protective structure, lacks raw materials protectiveness to lead to the stem cell thing to carry and easily take place the breakage when transporting, lead to its inside stem cell to spill over and cause the necrotic phenomenon of stem cell, cause the economic loss of certain degree to the user.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems in the prior art, the invention aims to provide a cell refrigerating box for long-distance transportation, which can realize that a sealing mechanism carries out floating clamping on a cell preservation box in the transportation process, when strong impact is applied, liquid nitrogen is released, after a buffer ring is broken, a released protective liquid covers a culture disc and is cooled and condensed by the liquid nitrogen, the culture disc is coated and sealed by the solidified cooling liquid, and the device is damaged, so that the released liquid nitrogen can still ensure long-time cooling and sealing of the cell preservation box after leakage, and long-time storage of cells is ensured.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A cell fridge for long-distance transport, includes the fridge main part, it has sealing door to articulate on the lateral wall of fridge main part, the one end joint that the sealing door was kept away from to the fridge main part has C type frame, it has the storage hole to cut in the fridge main part, a plurality of evenly distributed's cell preservation box has been placed in the storage hole, fixedly connected with a plurality of buffer rings with cell preservation box assorted on the inner wall in storage hole, buffer ring is located between two adjacent cell preservation boxes, C type frame includes the mount main part, the upper and lower both ends of mount main part have all been cut the mounting hole, the joint has the jam in the mounting hole, the lower extreme fixedly connected with pressure column of jam, the downside of pressure column is equipped with the splint that floats, be connected with compression spring between splint and the jam, compression spring cup joints on the pressure column, fixedly connected with pressure vessel in the mount main part, the buffering ring includes the diaphragm, a pair of curb plate of fixedly connected with on the diaphragm, be connected with the shunt tubes between pressure vessel and the diaphragm, install a plurality of evenly distributed's shower nozzle on the diaphragm, can realize that sealing mechanism floats the centre gripping to the cell preservation box in the transportation, when receiving strong impact, release liquid nitrogen makes the buffering ring break the back, and the protection liquid of its release covers the culture dish to be condensed by the liquid nitrogen cooling, make the culture dish by the coolant cladding of solidifying and sealed, and the device is impaired and still can guarantee the long-time cooling of cell preservation box after the liquid nitrogen that leads to the release reveals and seal, guarantees the long-time storage of cell.
Furthermore, the transverse plate and the side plate are both made of flexible PVC materials, a brittle glass tube is installed in the side plate, low-temperature ethanol is stored in the brittle glass tube, a plurality of uniformly distributed liquid discharge holes are formed in the side plate, and the side plate can be extruded and broken to release the low-temperature ethanol to cover the cell preservation box for disinfection and low-temperature protection.
Further, the cell preservation box includes the culture dish of a pair of mutual joint, and is a pair of be connected with the sealing ring that prevents frostbite between the culture dish, the cladding has the freeze-proof membrane on the surface of cell preservation box, it has a plurality of honeycomb grooves to cut on the freeze-proof membrane, guarantees that the cell preservation box has sufficient leakproofness.
Furthermore, the high-pressure tank is made of heat-insulating metal materials, a pressure pump is connected between the high-pressure tank and the shunt pipe, and a pair of buffers matched with the pressure pump are installed in the refrigerator body, so that the pressure pump can be protected when the refrigerator is impacted.
Furthermore, a plurality of elastic clamping balls are fixedly connected to the outer wall of the clamping plug, a plurality of semicircular clamping grooves matched with the elastic clamping balls are formed in the inner wall of the mounting hole, and the clamping plug is convenient to mount and dismount.
Furthermore, the bottom fixedly connected with pressure sensor of card stopper, the splint that floats with the relative one end fixedly connected with of card stopper and pressure sensor assorted pressure post, when the device receives the impact, on the splint that floats with pressure sensor and pressure post laminating for a long time to this makes pressure sensor send signal for the force pump, makes the force pump work and exports the liquid nitrogen in the high-pressure tank.
Furthermore, the refrigerator main body is made of materials, and a heat insulation layer is paved on the inner wall of the storage hole.
Furthermore, one end of the compression spring, which is far away from the pressure column, is fixedly connected with a heat insulation plate matched with the cell preservation box, and an elastic fiber buffer layer is paved on the heat insulation plate.
Furthermore, fixedly connected with control panel on the surface of refrigerator main part, the last controller that is connected with of control panel, high-pressure pump and pressure sensor all with controller signal connection.
A cell refrigerator for long-distance transportation comprises the following using methods:
s1, firstly, opening the sealing door, and then clamping the cell preservation box between a pair of buffer rings;
s2, after the sealing door is closed, the control panel is used for controlling the high-pressure pump to inject liquid nitrogen in the high-pressure tank into the storage hole, so that the upper side and the lower side of the cell preservation box are both coated by the liquid nitrogen;
s3, when the device is strongly vibrated in the using process, the pressure column on the compression spring is contacted with the pressure sensor, so that the controller controls the high-pressure pump to extract and inject liquid nitrogen phase in the high-pressure tank into the storage hole after receiving the feedback signal;
s4, when the device is further shocked or impacted, the compression spring applies strong pressure to the cell preservation boxes, so that the buffer ring between two adjacent cell preservation boxes is under strong extrusion force, the brittle glass in the side plate is broken, and after the liquid nitrogen in the device fails, the low-temperature ethanol flow in the side plate is released and covers the cell preservation boxes.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
(1) this scheme can realize that sealing mechanism floats the centre gripping to the cell preservation box in the transportation, when receiving strong impact, releases the liquid nitrogen, makes the buffer ring fracture back, and the protection liquid of its release covers the culture dish to be condensed by the liquid nitrogen cooling, make the culture dish by the coolant cladding of solidifying and sealed, and the device is impaired and still can guarantee the long-time cooling of cell preservation box after the liquid nitrogen that leads to the release is revealed and seal, guarantees the long-time storage of cell.
(2) The transverse plate and the side plate are both made of flexible PVC materials, the side plate is internally provided with a brittle glass tube, low-temperature ethanol is stored in the brittle glass tube, the side plate is provided with a plurality of uniformly distributed liquid discharge holes, and the side plate can release the low-temperature ethanol to cover the cell storage box for disinfection and low-temperature protection after being extruded and broken.
(3) Cell preservation box includes the culture dish of a pair of mutual joint, is connected with the sealing ring that prevents frostbite between a pair of culture dish, and the cladding has the frost-resistant membrane on cell preservation box's the surface, and it has a plurality of honeycomb recesses to cut on the frost-resistant membrane, guarantees that cell preservation box has sufficient leakproofness.
(4) The high-pressure tank is made of heat-insulating metal material, a pressure pump is connected between the high-pressure tank and the shunt pipe, and a pair of buffers matched with the pressure pump are arranged in the refrigerator main body, so that the pressure pump can be protected when the refrigerator is impacted.
(5) Bottom fixedly connected with pressure sensor of card stopper, the splint that floats and the relative one end fixedly connected with of card stopper and pressure sensor assorted pressure column, fixedly connected with control panel on the surface of refrigerator main part, the last controller that is connected with of control panel, high-pressure pump and pressure sensor all with controller signal connection, when the device receives the impact, on the splint that floats with pressure sensor and the laminating of pressure column for a long time to this makes pressure sensor send the signal for the force pump, make the force pump work export the liquid nitrogen in the high-pressure tank.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a cross-sectional view of the present invention;
FIG. 3 is a schematic view of the structure at A in FIG. 2;
FIG. 4 is a top view of the present invention;
FIG. 5 is a top cross-sectional view of the present invention;
fig. 6 is a partial sectional structure diagram of the side plate of the present invention.
The reference numbers in the figures illustrate:
1 refrigerator body, 2C type frame, 201 fixed frame body, 202 blocking plug, 203 pressure column, 204 floating clamp plate, 205 compression spring, 3 cell preservation box, 4 buffer ring, 401 horizontal plate, 402 side plate, 5 shunt tubes, 6 high pressure tank.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the connection can be direct connection or indirect connection through an intermediate medium, and can be communication inside the model adapting element. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1:
referring to fig. 1-3, a cell refrigerator for long distance transportation comprises a refrigerator body 1, a sealing door hinged to the side wall of the refrigerator body 1, a C-shaped frame 2 fastened to one end of the refrigerator body 1 away from the sealing door, a storage hole drilled in the refrigerator body 1, the refrigerator body 1 made of a material, and a thermal insulation layer laid on the inner wall of the storage hole. The outer surface of the refrigerator main body 1 is fixedly connected with a control panel, the control panel is connected with a controller, and the high-pressure pump and the pressure sensor are in signal connection with the controller. Placed a plurality of evenly distributed's cell preservation box 3 in the holding hole, fixedly connected with is a plurality of on the inner wall in holding hole with cell preservation box 3 assorted buffering ring 4, buffering ring 4 is located between two adjacent cell preservation boxes 3, cell preservation box 3 includes the culture dish of a pair of mutual joint, be connected with the sealing ring that prevents frostbite between a pair of culture dish, the cladding has the freeze-proof membrane on cell preservation box 3's the surface, it has a plurality of cellular recesses to cut on the freeze-proof membrane, guarantee that cell preservation box 3 has sufficient leakproofness.
Referring to fig. 2-6, the C-shaped frame 2 includes a main frame 201, mounting holes are drilled at upper and lower ends of the main frame 201, a clamping plug 202 is clamped in the mounting holes, a pressure column 203 is fixedly connected to a lower end of the clamping plug 202, a floating clamp plate 204 is disposed at a lower side of the pressure column 203, a compression spring 205 is connected between the floating clamp plate 204 and the clamping plug 202, the compression spring 205 is sleeved on the pressure column 203, a high pressure tank 6 is fixedly connected to the main frame 201, the high pressure tank 6 is made of a heat insulation metal material, a pressure pump is connected between the high pressure tank 6 and a flow dividing pipe 5, a pair of buffers matched with the pressure pump are installed in the main body 1 of the refrigerator to protect the pressure pump when the device is impacted, the buffer ring 4 includes a horizontal plate 401, a pair of side plates 402 is fixedly connected to the horizontal plate 401, the flow dividing pipe 5 is connected between the high pressure tank 6 and the horizontal plate 401, a plurality of uniformly distributed nozzles are installed on the horizontal plate 401, the transverse plate 401 and the side plate 402 are both made of flexible PVC materials, a brittle glass tube is mounted in the side plate 402 and stores low-temperature ethanol, a plurality of uniformly distributed liquid discharge holes are drilled in the side plate 402, and the purpose that the low-temperature ethanol can be released to cover the cell preservation box 3 for disinfection and low-temperature protection after the side plate 402 is crushed by extrusion can be achieved.
Referring to fig. 2-5, a plurality of elastic locking balls are fixedly connected to the outer wall of the locking plug 202, and a plurality of semicircular locking grooves matched with the elastic locking balls are formed in the inner wall of the mounting hole, so that the locking plug 202 can be conveniently mounted and dismounted. The bottom fixedly connected with pressure sensor of card stopper 202, the one end fixedly connected with that the splint 204 floats is relative with card stopper 202 with pressure sensor assorted pressure column, when the device receives the impact, on the splint 204 floats with pressure sensor and pressure column laminating for a long time to this makes pressure sensor send the signal for the force pump, makes the force pump work and exports the liquid nitrogen in the high-pressure tank 6. One end of the compression spring 205 far away from the pressure column 203 is fixedly connected with a heat insulation plate matched with the cell preservation box 3, and an elastic fiber buffer layer is paved on the heat insulation plate.
A cell refrigerator for long-distance transportation comprises the following using methods:
s1, firstly, the sealing door is opened, and then the cell preservation box 3 is clamped between the pair of buffer rings 4;
s2, after the sealing door is closed, the control panel is used for controlling the high-pressure pump to inject liquid nitrogen in the high-pressure tank 6 into the storage hole, so that the upper side and the lower side of the cell preservation box 3 are both coated by the liquid nitrogen;
s3, in the using process, after the device is subjected to strong vibration, the pressure column on the compression spring 205 is contacted with the pressure sensor, so that the controller controls the high-pressure pump to extract and inject liquid nitrogen phase storage holes in the high-pressure tank 6 after receiving a feedback signal;
s4, when the device is further shocked or impacted, the compression spring 205 applies strong pressure to the cell preservation boxes 3, so that the buffer ring 4 between two adjacent cell preservation boxes 3 is subjected to strong extrusion force, so that the brittle glass in the side plate 402 is broken, and after the liquid nitrogen in the device is invalid, the low-temperature ethanol flow in the side plate 402 is released and covers the cell preservation boxes 3.
This scheme main part is by a plurality of cell preservation box 3 at the incasement, the centre gripping that floats to cell preservation box 3 of splint 204 is moved on C type frame 2 in the transportation, buffer space has between two adjacent cell preservation box 3, the device is when receiving strong impact, release liquid nitrogen, make the back of buffer ring 4 breaking, the alcohol of its release covers cell preservation box 3, and condense by the liquid nitrogen cooling, make cell preservation box 3 by the alcohol cladding of solidifying and sealed, alcohol still disinfects the cell preservation box 3 outside when carrying out low temperature protection to the cell preservation box 3 outside, prevent to permeate pollutant pollution cell preservation box 3 in the device, and the device is impaired and still can guarantee the long-time cooling of cell preservation box 3 after the liquid nitrogen that leads to releasing is revealed and is sealed, guarantee the long-time storage of cell.
The foregoing is only a preferred embodiment of the present invention; the scope of the invention is not limited thereto. Any person skilled in the art should be able to cover the technical scope of the present invention by equivalent or modified solutions and modifications within the technical scope of the present invention.