CN111685104B - Stem cell low-temperature storage device - Google Patents
Stem cell low-temperature storage device Download PDFInfo
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- CN111685104B CN111685104B CN202010652483.9A CN202010652483A CN111685104B CN 111685104 B CN111685104 B CN 111685104B CN 202010652483 A CN202010652483 A CN 202010652483A CN 111685104 B CN111685104 B CN 111685104B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
- A01N1/0257—Stationary or portable vessels generating cryogenic temperatures
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Abstract
The invention discloses a stem cell low-temperature preservation device in the field of stem cell preservation, which comprises a heat preservation outer cylinder and a storage inner cylinder, wherein the top end of the inner wall of the heat preservation outer cylinder is provided with connecting threads, the top end of the inner wall of the heat preservation outer cylinder is connected with a threaded closing part through the connecting threads, the center of the bottom end of the inner cavity of the heat preservation outer cylinder is fixedly provided with a connecting rod, the outer side wall of the heat preservation outer cylinder is provided with an inlet and an outlet, the inlet is internally provided with an inlet pipe, the outlet is internally provided with an outlet pipe, the inner wall of the heat preservation outer cylinder is symmetrically provided with two groups of chutes, the top ends and the bottom ends of the chutes are respectively provided with a bearing, an adjusting screw rod is rotatably connected between the bearings, the inner part of each chute is slidably connected with a slide, the inside of the closed bearing rotates to form a rotating rod, and the bottom end of the rotating rod is fixedly connected with the top end of the adjusting screw rod.
Description
Technical Field
The invention relates to the field of stem cell preservation, in particular to a stem cell low-temperature preservation device.
Background
Stem cells are a type of pluripotent cells having a self-renewal ability, which can be differentiated into a variety of pluripotent cells under certain conditions, and are a type of primitive cells having self-replication and multipotent differentiation potential, i.e., stem cells that maintain an unoriented differentiation state and have a proliferation ability, which can be differentiated into a variety of functional cells or tissue organs under suitable conditions or given suitable signals, and are classified into embryonic stem cells and adult stem cells according to the stage of development in which the stem cells are present. The stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent stem cells. Stem cells are insufficiently differentiated and immature cells, have potential functions of regenerating various tissues, organs and human bodies, and are called universal cells in the medical field. Stem cells require a low temperature environment for storage. The currently more common method of cryopreservation is vitrification, during which a sample of cells suspended in a vitrification preservation medium is exposed to cryogenic fluids or other freezing media, such as liquid nitrogen, either by direct invasion or indirectly.
At present, chinese patent application No. CN201921099903.4 discloses a stem cell cryopreservation apparatus, which includes: the device comprises a heat-preserving barrel and a storage barrel, wherein a fixed groove is formed in the heat-preserving barrel, the storage barrel is installed in the fixed groove, mounting holes are formed in two side walls of the heat-preserving barrel, a liquid nitrogen groove is formed in the heat-preserving barrel, the liquid nitrogen groove is U-shaped, two ends of the liquid nitrogen groove are respectively communicated with the mounting holes, threads are arranged on the inner wall of each mounting hole, and a hole plug is connected to the inner portion of each mounting; the top of the storage barrel is provided with a first limiting plate and a second handle, the second handle is vertically fixed on the top surface of the first limiting plate, the inner wall of the heat-preservation barrel is symmetrically provided with mounting grooves, springs are fixed in the mounting grooves, clamping blocks are mounted at the tail ends of the springs, and inwards-recessed clamping grooves are formed in opposite surfaces of the clamping blocks. The device places the storage barrel with the stem cells inside for heat preservation, and has small integral volume and light weight, thereby being convenient for carrying; the shaking generated by storing the barrel in the moving process can be reduced, and the barrel is always attached to the inner wall of the heat-preserving barrel. However, when the device is used for stem cell cryopreservation, the cooling liquid nitrogen is separated from the storage barrel and is not in direct contact with the storage barrel, so that the time for achieving the required low temperature is long. Based on the above, the invention designs a stem cell cryopreservation device.
Disclosure of Invention
The invention aims to provide a stem cell cryopreservation device, which aims to solve the problem that the time for achieving the required low temperature is long because the device is used for cryopreservation of stem cells, and cooling liquid nitrogen is separated from a storage barrel and is not in direct contact with the storage barrel.
In order to achieve the purpose, the invention provides the following technical scheme:
a stem cell cryopreservation device comprises a heat-preservation outer barrel and a storage inner barrel, wherein connecting threads are arranged at the top end of the inner wall of the heat-preservation outer barrel, the top end of the inner wall of the heat-preservation outer barrel is connected with a thread sealing part through the connecting threads, a handle is fixed at the center of the top end of the thread sealing part, a connecting rod is fixed at the center of the bottom end of an inner cavity of the heat-preservation outer barrel, an inlet and an outlet are arranged on the outer side wall of the heat-preservation outer barrel, an inlet pipe is arranged in the inlet, an outlet pipe is arranged in the outlet, two groups of sliding grooves are symmetrically arranged on the inner wall of the heat-preservation outer barrel, bearings are arranged at the top end and the bottom end of each sliding groove, an adjusting screw rod is rotatably connected between the bearings, a sliding block is slidably connected, the bottom center of the storage inner cylinder is provided with a connecting hole matched with the connecting rod, auxiliary strips are symmetrically fixed at the top end of the side wall of the storage inner cylinder, a closed rubber plug is plugged at the top end of the storage inner cylinder, a closed bearing is embedded at the top end of the heat-preservation outer cylinder, a rotating rod is rotated inside the closed bearing, and the bottom end of the rotating rod is fixedly connected with the top end of the adjusting screw rod.
Preferably, the height of the threaded closing part is equal to that of the connecting thread, the inlet and the outlet are both arranged at the bottom of the side wall of the heat-preservation outer barrel, the horizontal plane of the bottom of the inlet is slightly higher than the horizontal plane of the bottom of the inner cavity of the heat-preservation outer barrel, and the horizontal plane of the bottom of the outlet is slightly lower than the horizontal plane of the bottom of the inner cavity of the heat-preservation outer barrel.
Preferably, the length of the sliding groove is greater than half of the height of the inner cavity of the heat-preservation outer barrel, and the vertical distance from the top end of the sliding groove to the top end of the heat-preservation outer barrel is equal to the height of the connecting thread.
Preferably, the length of the adjusting screw is equal to that of the sliding groove, and the threads on the surface of the adjusting screw are arranged on the part, located between the upper and lower groups of bearings, of the surface wall of the adjusting screw.
Preferably, the end face of the sliding block is an arc face matched with the arc of the inner wall of the heat-preservation outer barrel, and the top end and the bottom end of the limiting sleeve disk are flush with the top end and the bottom end of the sliding block respectively.
Preferably, the horizontal plane of the bottom end of the liquid level sensor and the horizontal plane of the bottom end of the limiting sleeve disc are the same horizontal plane, the height of the connecting hole is equal to that of the connecting rod, and the top end of the auxiliary strip is flush with the top end of the storage inner cylinder.
Preferably, the length of the rotating rod is larger than the vertical distance from the top end of the sliding groove to the top end of the heat-preservation outer barrel, and the diameter of the rotating rod is smaller than that of the adjusting screw.
Compared with the prior art, the invention has the beneficial effects that: through the arrangement of the sliding groove, the sliding block, the adjusting screw rod and the rotating rod, the rotating rod can be used for driving the adjusting screw rod to rotate, and the sliding block is driven to move in the sliding groove under the threaded fit of the adjusting screw rod and the sliding block, so that the limiting sleeve disc can move, the position of the limiting sleeve disc can be adjusted, and the device is suitable for placing the storage inner cylinders with various heights; the storage inner cylinder can be stably placed in the heat-insulation outer cylinder by the aid of the insertion fit of the connecting rod and the connecting hole and the combined action of the connecting rod and the elastic layer in the limiting sleeve disc; through setting up into pipe and exit tube, the cooling liquid nitrogen gets into in the heat preservation urceolus through advancing the pipe and directly contacts with depositing the inner tube for it reaches the time of required microthermal to deposit the inner tube and reduces.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings described below are only some examples of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic cross-sectional view of the present invention;
FIG. 3 is a schematic view of the internal structure of the heat-insulating outer cylinder of the present invention;
FIG. 4 is a schematic view of the external structure of the heat-insulating outer cylinder of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1-heat preservation outer cylinder, 2-storage inner cylinder, 3-connecting thread, 4-thread sealing piece, 5-handle, 6-connecting rod, 7-inlet, 8-inlet pipe, 9-outlet, 10-outlet pipe, 11-chute, 12-bearing, 13-adjusting screw, 14-sliding block, 15-limiting sleeve disc, 16-elastic layer, 17-liquid level sensor, 18-connecting hole, 19-auxiliary strip, 20-sealing rubber plug, 21-sealing bearing and 22-rotating rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the examples of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution:
as shown in figures 1 and 2, a stem cell cryopreservation device comprises a heat-preservation outer cylinder 1 and a storage inner cylinder 2, wherein the top end of the inner wall of the heat-preservation outer cylinder 1 is provided with a connecting thread 3, the top end of the inner wall of the heat-preservation outer cylinder 1 is in threaded connection with a threaded closing part 4 through the connecting thread 3, the height of the threaded closing part 4 is equal to that of the connecting thread 3, the center of the top end of the threaded closing part 4 is fixedly provided with a handle 5, the center of the bottom end of an inner cavity of the heat-preservation outer cylinder 1 is fixedly provided with a connecting rod 6, the outer side wall of the heat-preservation outer cylinder 1 is provided with an inlet 7 and an outlet 9, the inlet 7 and the outlet 9 are both arranged at the bottom end of the side wall of the heat-preservation outer cylinder 1, the bottom end of the inlet 7 is slightly higher, two groups of sliding grooves 11 are symmetrically formed in the inner wall of the heat-preservation outer barrel 1, the length of each sliding groove 11 is larger than half of the height of the inner cavity of the heat-preservation outer barrel 1, and the vertical distance from the top end of each sliding groove 11 to the top end of the heat-preservation outer barrel 1 is equal to the height of the connecting thread 3;
as shown in fig. 2, 3 and 4, bearings 12 are arranged at the top end and the bottom end of a sliding chute 11, an adjusting screw 13 is rotatably connected between the bearings 12, the length of the adjusting screw 13 is equal to that of the sliding chute 11, threads on the surface of the adjusting screw 13 are arranged on the part of the surface wall of the adjusting screw 13, which is positioned between the upper and the lower groups of bearings 12, a sliding block 14 is slidably connected inside the sliding chute 11, the end surface of the sliding block 14 is an arc surface matched with the arc of the inner wall of the heat-insulating outer cylinder 1, the threads of the adjusting screw 13 penetrate through the sliding block 14, a position-limiting sleeve disk 15 is fixed on the side surface of the sliding block 14, the top end and the bottom end of the position-limiting sleeve disk 15 are respectively flush with the top end and the bottom end of the sliding block 14, an elastic layer 16 is arranged on the inner wall of the position-limiting sleeve disk 15, a liquid level sensor 17 is embedded at the bottom, the height that highly equals connecting rod 6 of connecting hole 18, it is fixed with supplementary strip 19 to deposit 2 lateral wall top symmetries of inner tube, 19 tops of supplementary strip and deposit 2 top parallel and level of inner tube, it is equipped with sealed plug 20 to deposit 2 top stopper of inner tube, 1 top of heat preservation urceolus is inlayed and is equipped with sealed bearing 21, the inside rotation of sealed bearing 21 has bull stick 22, bull stick 22 bottom and adjusting screw 13 top are fixed continuous, the length of bull stick 22 is greater than the perpendicular distance on 11 tops of spout to 1 top of heat preservation urceolus, the diameter of bull stick 22 is less than adjusting screw 13's diameter.
One specific application of this embodiment is: according to the height of the storage inner cylinder 2 to be placed, the rotating rod 22 is rotated to drive the adjusting screw 13 to rotate, the sliding block 14 is driven to move under the threaded matching of the adjusting screw 13 and the sliding block 14, so that the position of the limiting sleeve disc 15 is adjusted, the height of the limiting sleeve disc is adjusted, the storage inner cylinder 2 filled with stem cells is placed into the heat-preservation outer cylinder 1, the storage inner cylinder 2 is positioned by the inserting matching of the connecting rod 6 and the connecting hole 18 in combination with the elastic layer 16, the storage inner cylinder 2 is stably placed in the heat-preservation outer cylinder 1, then the heat-preservation outer cylinder 1 is sealed by the matching of the connecting thread 3 and the threaded sealing part 4, finally, cooling liquid nitrogen is input into the heat-preservation outer cylinder 1 through the external cooling liquid nitrogen input device of the inlet pipe 8, liquid level control is performed by the liquid level sensor 17, the storage inner cylinder 2 is directly contacted with the cooling liquid nitrogen for cooling, the screw closure 4 is opened and the storage inner container 2 is removed by means of the auxiliary strip 19.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (4)
1. The utility model provides a stem cell cryopreservation device, includes heat preservation urceolus (1) and deposits inner tube (2), its characterized in that: the heat-insulation outer barrel is characterized in that a connecting thread (3) is formed in the top end of the inner wall of the heat-insulation outer barrel (1), the top end of the inner wall of the heat-insulation outer barrel (1) is connected with a thread sealing part (4) through the connecting thread (3), a handle (5) is fixed at the center of the top end of the thread sealing part (4), a connecting rod (6) is fixed at the center of the bottom end of the inner cavity of the heat-insulation outer barrel (1), an inlet (7) and an outlet (9) are formed in the outer side wall of the heat-insulation outer barrel (1), an inlet pipe (8) is installed in the inlet (7), an outlet pipe (10) is installed in the outlet (9), two groups of sliding chutes (11) are symmetrically formed in the inner wall of the heat-insulation outer barrel (1), bearings (12) are arranged at the, the adjusting screw (13) penetrates through the sliding block (14) in a threaded manner, a limiting sleeve disc (15) is fixed on the side face of the sliding block (14), an elastic layer (16) is arranged on the inner wall of the limiting sleeve disc (15), a liquid level sensor (17) is embedded at the bottom end of the limiting sleeve disc (15), a connecting hole (18) matched with the connecting rod (6) is formed in the center of the bottom end of the storing inner cylinder (2), auxiliary strips (19) are symmetrically fixed at the top end of the side wall of the storing inner cylinder (2), a sealing rubber plug (20) is plugged at the top end of the storing inner cylinder (2), a sealing bearing (21) is embedded at the top end of the heat-insulating outer cylinder (1), a rotating rod (22) is rotatably arranged in the sealing bearing (21), and the bottom end of the rotating rod;
the height of the threaded sealing part (4) is equal to that of the connecting thread (3), the inlet (7) and the outlet (9) are both arranged at the bottom end of the side wall of the heat-preservation outer cylinder (1), the horizontal plane of the bottom end of the inlet (7) is slightly higher than the horizontal plane of the bottom end of the inner cavity of the heat-preservation outer cylinder (1), and the horizontal plane of the bottom end of the outlet (9) is slightly lower than the horizontal plane of the bottom end of the inner cavity of the heat-preservation outer cylinder (1);
the end face of the sliding block (14) is an arc face matched with the arc of the inner wall of the heat-preservation outer cylinder (1), and the top end and the bottom end of the limiting sleeve disc (15) are respectively flush with the top end and the bottom end of the sliding block (14);
the horizontal plane of the bottom end of the liquid level sensor (17) and the horizontal plane of the bottom end of the limiting sleeve disc (15) are the same horizontal plane, the height of the connecting hole (18) is equal to that of the connecting rod (6), and the top end of the auxiliary strip (19) is flush with the top end of the storage inner cylinder (2).
2. The stem cell cryopreservation apparatus of claim 1, wherein: the length of the sliding groove (11) is greater than half of the height of the inner cavity of the heat-preservation outer barrel (1), and the vertical distance from the top end of the sliding groove (11) to the top end of the heat-preservation outer barrel (1) is equal to the height of the connecting thread (3).
3. The stem cell cryopreservation apparatus of claim 1, wherein: the length of the adjusting screw rod (13) is equal to that of the sliding chute (11), and threads on the surface of the adjusting screw rod (13) are arranged on the surface wall of the adjusting screw rod (13) and are positioned on an upper group of bearings and a lower group of bearings (C)
12) The section in between.
4. The stem cell cryopreservation apparatus of claim 1, wherein: the length of the rotating rod (22) is greater than the vertical distance from the top end of the sliding groove (11) to the top end of the heat-preservation outer cylinder (1), and the diameter of the rotating rod (22) is smaller than that of the adjusting screw rod (13).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010652483.9A CN111685104B (en) | 2020-07-08 | 2020-07-08 | Stem cell low-temperature storage device |
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| CN202010652483.9A CN111685104B (en) | 2020-07-08 | 2020-07-08 | Stem cell low-temperature storage device |
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| CN111685104B true CN111685104B (en) | 2021-02-19 |
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| CN112354575B (en) * | 2020-11-18 | 2022-04-01 | 珠海市康利莱医疗器械有限公司 | Production device and process suitable for heparin anticoagulant tube |
| CN113089726A (en) * | 2021-03-11 | 2021-07-09 | 浙江司邦齐环境科技有限公司 | Anti-collapse pipe fitting type inspection well for soft geology and installation method |
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