CN113349226A

CN113349226A - Application of rose oil in pest control

Info

Publication number: CN113349226A
Application number: CN202110579249.2A
Authority: CN
Inventors: 程东美; 周易; 何俊烺; 何景超
Original assignee: Zhongkai University of Agriculture and Engineering
Current assignee: Zhongkai University of Agriculture and Engineering
Priority date: 2021-05-26
Filing date: 2021-05-26
Publication date: 2021-09-07

Abstract

The invention relates to the technical field of pesticides, in particular to application of rose hip oil in pest control, and provides application of rose hip oil in dissolving pest wax secretion in pest control of scale insects, wherein the rose hip oil can effectively dissolve the wax secretion of the scale insects; the invention provides a pesticide preparation, which comprises rose hip oil and a pesticide, can effectively improve the penetrability of the pesticide on pests, improve the ductility and the adhesive force of the pesticide and improve the insecticidal effect of the unit pesticide; the invention is an application of a pesticide preparation, and the pesticide preparation has excellent effect on preventing and controlling scale insects; the invention provides a preparation method of an insecticide preparation, which has the advantage of easy operation.

Description

Application of rose oil in pest control

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to application of rose hip oil in pest control.

Background

The plant essential oil is a secondary metabolite, exists in different tissues of plants, is in a liquid state at normal temperature, is volatile, is insoluble in water, and is soluble in an organic solvent. Plant essential oils are widely used as essences and spices in the perfume and food industries, the essence of which is known for repelling insects. The main component of the plant essential oil is a haploid hemiterpene compound with high volatility, and the worry about residue problem is reduced due to the high volatility of the plant essential oil.

However, plant essential oils are used for controlling pests in pest repelling applications only by repelling them, and they are not effective in killing pests, so that the use of insecticides for killing pests is still an important way in pest control.

The pesticide is mainly aqueous solution, however, the pesticide has limitations in controlling the coccid pests: the scale insects can generate various waxy secretions (hereinafter referred to as waxy secretions), the waxy secretions wrap the bodies of the scale insects, the waxy secretions can repel aqueous solution when the scale insects are prevented and controlled, the scale insects are immobile for a long time, and the scale insects can not easily contact pesticides attached to branches and leaves. Therefore, the prevention and control of the coccid pests at present mainly depend on natural prevention and control of natural enemies, including ladybug family, phlebotomidae family, parasitic wasps and the like. However, because of the enormous number of coccid pests, it is obvious that effective control can be achieved by means of natural enemies.

Disclosure of Invention

The first purpose of the invention is to avoid the defects in the prior art and provide the application of the rose hip oil in dissolving insect wax secretion in preventing and controlling scale insects.

Provides an application of rose hip oil in dissolving wax secretion in preventing and treating scale insects.

Further, the scale insect is Egypt icerya purchasi.

The application of the rose hip oil in preventing and treating scale insects for dissolving pest wax secretion has the beneficial effects that:

the rose hip oil contains linoleic acid and linolenic acid capable of dissolving organic matters, and the linoleic acid and the linolenic acid have obvious dissolving effect on the body surface wax of the Egyptian icerya, can reduce or eliminate the protection effect of wax secretion such as scale shells and the like on scale insects, increase the contact surface and the dosage of an insecticide and the scale insects, and obviously improve the action speed and the prevention and treatment effect of the insecticide.

The second object of the present invention is to provide an insecticide which is effective in improving the penetration of an insecticide into pests, improving the ductility and adhesion of an insecticide, and improving the insecticidal effect per unit of insecticide.

The second purpose of the invention is realized by the following technical scheme:

an insecticide preparation is provided, which comprises rose hip oil and insecticide.

Further, the insecticide is matrine.

Further, the mass ratio of the matrine to the rose hip oil is 1: 10-1: 500.

further, the mass ratio of the matrine to the rose hip oil is 1: 50.

when the mass ratio of the matrine to the rose hip oil is 1: at 50 deg.C, the rose-hip oil is used in minimum amount, but the best insecticidal effect can be obtained.

The insecticide preparation has the beneficial effects that:

(1) the rose hip oil can dissolve wax secretion outside the body wall of pests, increases the penetration of pesticide on the body wall of the pests, and improves the pesticidal effect of the pesticide.

(2) After the rose hip oil and the insecticide are mixed, the surface tension of the insecticide liquid can be reduced, the drying time can be shortened, the adhesive force of the insecticide on the surface of a plant and the surface of a pest is improved, the use of a chemical solution is facilitated, and the control effect is improved.

(3) The rose hip oil can improve the extension diameter of the insecticide, is beneficial to the insecticide to diffuse on the surface of the Erypris pelagi, and can enable the active ingredients to better contact with insects and improve the control effect of the insecticide.

(4) The rose fruit oil can improve the maximum retention of the pesticide, improve the adhesion of the pesticide on the surfaces of leaves and pests, and reduce the waste caused by the falling of the pesticide liquid in the using process, thereby reducing the pesticide consumption, reducing the pesticide residue, relieving the environmental pressure, effectively delaying the generation of the drug resistance of the pests, reducing the control cost, and having good economic benefit and ecological benefit.

(5) The linoleic acid and the linolenic acid of the rose fruit oil are insoluble in water, and the rose fruit oil and the effective components of the pesticide have good synergistic interaction effect but no cross resistance, so that the insecticidal effect of the pesticide can be effectively maintained.

The third purpose of the invention is to provide the application of the insecticide preparation, and the insecticide preparation has excellent effect on preventing and controlling the scale insects by adopting the application of the insecticide preparation in preventing and controlling the scale insects.

The fourth purpose of the invention is to provide a preparation method of the insecticidal preparation, and the preparation method comprises the following steps of mixing the rose hip oil and the insecticidal preparation.

Drawings

The invention is further illustrated by means of the attached drawings, but the embodiments in the drawings do not constitute any limitation to the invention, and for a person skilled in the art, other drawings can be derived on the basis of the following drawings without inventive effort.

FIG. 1 is a graph showing the dissolution effect of the different solutions of experiment 1 on the wax secretion of Erigerus pela.

FIG. 2 is a graph showing the results of the experiment 5, which shows the ratio of matrine to pass through the wax secretion of Erianthus pelagicus after the treatment of rosehip oil.

FIG. 3 is a graph of pest mortality data for different mass ratios of matrine to the rose hip oil for pest control.

Detailed Description

The invention is further described with reference to the following examples.

Example 1

The embodiment discloses an insecticide preparation, which comprises rose hip oil and an insecticide, wherein the insecticide is an insecticide raw material which can be directly purchased in the market. Preferably, the pesticide is matrine. In practical application, the rose hip oil can be combined with other pesticides for use, so as to achieve the effect of killing insects and enhancing the effect.

Wherein the mass ratio of the matrine to the rose hip oil is 1: 50.

treating pests by adopting matrine and the rose hip oil with different mass ratios to obtain a data table shown in figure 3, wherein when the mass ratio of the matrine to the rose hip oil is 1: at 50 hours, the best insecticidal effect can be obtained with the least dosage of the rose hip oil, and the mass ratio of the insecticidal effect to the matrine to the rose hip oil is 1: 100 or 1: the difference of the mortality rate of the pests is not obvious when the pest is 500 hours, and the weight ratio of the matrine to the rose hip oil is 1: 50 can not only save the using amount of the rose oil, but also achieve the best insecticidal effect.

The preparation method of the insecticidal preparation of the embodiment comprises the following steps: mixing the palmarosa oil and the pesticide.

Example 2

The difference between the present embodiment and embodiment 1 is that the mass ratio of matrine to rose hip oil in the present embodiment is 1: 10.

example 3

The difference between the present embodiment and embodiment 1 is that the mass ratio of matrine to rose hip oil in the present embodiment is 1: 500.

example 4

The difference between the present embodiment and embodiment 1 is that the mass ratio of matrine to rose hip oil in the present embodiment is 1: 60.

performance testing

In order to clearly illustrate the technical effects of the invention, the following experiments take Erythium gracile as an experimental object, and other scale insects can be used in the practical use.

Description of the materials

A test insect source: the Ergyptian icerya is collected from the Magnolia garden of the southern agricultural university of Guangzhou, China and the Macaranga tanarius, and female adults with consistent health conditions and sizes are selected for testing.

Essential oil for testing: rose hip oil was purchased from Jiangxi Shenshen botanical spices, Inc.

Test insecticide: matrine (> 98%) was purchased from Shanghai Allantin Biotechnology Ltd.

Experimental methods

Experiment 1: determination of dissolution of wax secretion of Erythrophlomis gracilis by rose hip oil

And 5 mu L of rose hip oil is taken by an adjustable pipette and dropped on the middle back of the chest of the Egyptian icerya. The dissolution of the essential oil on the wax secretions was observed and photographed. The control group was treated with equal volume of ethanol and water. Drying treated insects for 2h in vacuum, selecting appropriate test insects under a stereoscope, spraying gold for 2min under a vacuum condition, and observing under a scanning electron microscope.

Through experiment 1, a dissolving effect diagram of wax secretion of Erysia pela 5min after different solutions are treated is obtained, wherein numbers a-c in the diagram 1 are photos of water, ethanol and rosehip oil treatment in sequence, and numbers d-f are scanning electron microscope images of water, ethanol and rosehip oil treatment in sequence.

As can be seen from the figure 1, the wax secretion of the Egyptian icerya scales has a repulsive effect on water, the ethanol does not dissolve the wax secretion, and the rose hip oil has a good dissolving effect on the wax secretion, wherein after the rose hip oil is treated for 5min, the wax secretion on the body surface of the test insect disappears, and an orange red insect body can be seen, and the result shows that the rose hip oil has a dissolving effect on the wax secretion on the body surface of the Egyptian icerya scales. The wax threads on the surface of the worm body after being treated by water and ethanol are densely distributed on the surface of the worm body when being observed under a scanning electron microscope, and the wax threads on the surface of the worm body after being treated by the rose hip oil are dissolved. The result shows that the rose hip oil has good dissolving effect on wax secretion of Erypnia elapidium.

Experiment 2: determination of contact toxicity of rose hip oil on female adult Lecanicillium lecanii

The contact toxicity of the rose hip oil on female adults of Elapidae blepharismacorus was determined by a drop method. The rose hip oil is prepared into 5 series of concentrations (1250mg/L, 2500mg/L, 5000mg/L, 10000mg/L and 20000mg/L) by ethanol, and the prepared rose hip oil ethanol solution is respectively dripped on the back of the middle chest of female adults of Erysia pelagica with a pipette, and the ethanol is used as a contrast.

Each group is provided with 3 replicates, 10 test insects are placed in each culture dish, and 5 mu L of each test insect is dripped. The treated test insects with leaves are placed in a culture dish paved with wet filter paper, and the death result of the test insects is checked every 12 hours.

Criteria for death: the standard is that the test insects are immobile when the brush pen is touched.

The data shown in Table 1 are obtained, Table 1 is as follows:

TABLE 1 contact killing Activity of Rose hip oil on female adults of Eleutherococcus aegypti

As shown in Table 1, the contact activity of the rose hip oil on female adults of Erysia pelykii increases with the concentration of the rose hip oil, and the insecticidal activity is enhanced with the increase of time. The 72h corrected mortality at a treatment concentration of 20000mg/L was 77.78%. This indicates that the insecticidal activity of the rose hip oil on Erythium amansii is not strong, and the contact killing effect is not obvious when the rose hip oil is used alone as an insecticide.

Experiment 3: determination of contact toxicity of matrine to female Egyptian Lepidium

The contact toxicity of matrine to female adult Lecanicillium lecanii is determined by a drop method. The matrine is prepared into 5 series of concentrations (5mg/L, 10mg/L, 20mg/L, 40mg/L and 80mg/L) by ethanol, and matrine solution with each concentration is respectively dropped on the back of the middle chest of female adult Lecanicillium lecanii by a pipette, and ethanol is used as a reference.

The data shown in Table 2 are obtained, Table 2 is as follows:

TABLE 2 contact killing activity of matrine on female Egyptian Blackermum

As shown in Table 2, the contact activity of matrine on female imagoes of Erythrophlia gracilis increases with the increase of the concentration of the rose hip oil, and the insecticidal activity increases with the increase of time. The 72h corrected mortality rates at concentrations of 80mg/L and 160mg/L were 61.11% and 74.07%, respectively, since matrine does not poison the respective pests directly and completely, but controlling pest biota numbers does not seriously affect the production and reproduction of the plant population.

Experiment 4: determination of contact toxicity of rose hip oil and matrine in combination on female adult Egyptian Blasty scales

The contact toxicity of the combination of the rosehip oil and the matrine on female adults of the Erysia pelykii is determined by adopting a dropping method. Mixing 5 matrine solutions (5mg/L, 10mg/L, 20mg/L, 40mg/L, 80mg/L) with matrine and rosehip oil of 1: adding 50% of rose oil. The matrine solution with each concentration was dropped on the dorsal surface of the middle breast of female adult Lecanicillium lecanii with a pipette gun, and matrine treatment was used as a control.

The data shown in tables 3 and 4 were obtained, and tables 3 and 4 were as follows:

TABLE 3 contact killing activity of rose hip oil and matrine in combination on female Egyptian Blasty scales

TABLE 4 contact killing activity of rose hip oil and matrine in combination on female Egyptian Blasty scales

As shown in Table 3, when the matrine is used together with the rose hip oil, the death rate after 72h correction is 94.44% when the concentration of the matrine is 80mg/L, and the effect is obviously improved compared with the single use of the matrine. As is evident from Table 4, LC was observed when matrine alone was used₅₀60.72mg/L, LC after adding rose hip oil₅₀Is 13.68mg/L, which shows that the rose hip oil improves the prevention and treatment effect of the matrine on Egyptian icerya and improves the drug effect of the matrine.

Experiment 5: determination of matrine penetration ratio of Egyptian Blastus wax secretion after rose hip oil treatment

The test insects were spotted with 5. mu.L of 40mg/L matrine, matrine + RO (1: 50, c/c), and 50 test insects were treated each for penetration test. Eluting with 70% ethanol for 30s, repeating for 3 times, taking out polypide, centrifuging at 15000r/min for 10min, collecting supernatant, freeze drying, adding 1ml acetonitrile, ultrasonic dissolving, and filtering with 0.22 μm filter membrane. The content of matrine was analyzed by high performance liquid chromatography using a Nippon Shimadzu LC-20A variable wavelength UV-visible detector. Eclipse XDB-C18 column (150mm 4.6mm,5 μm); mobile phase: acetonitrile-pbs (0.2mmol) (20: 80, v/v); flow rate: 1.0mL min-1; column temperature: 30, of a nitrogen-containing gas; detection wavelength: 210 nm; injection amount: 20 μ l, 3 replicates per treatment.

As shown in FIG. 2, the penetration rate of matrine on the wax secretion of Erythium gracilis increased significantly after the addition of rosehip oil, and the penetration rate also increased with the increase of time. The penetration rate of the matrine used alone is 6.92%, and the penetration rate reaches 33.64% at the maximum after 4h of adding the rose hip oil, which is improved by 26%.

Experiment 6: determination of physical Properties of solution after treatment with Rose hip oil

Surface tension: the rose hip oil was diluted with ethanol to 5 concentrations (1250mg/L, 2500mg/L, 5000mg/L, 10000mg/L, 20000mg/L) and the surface tension was measured by a surface tension meter, and each treatment was repeated 3 times.

Drying time: diluting the Rosa rugosa Thunb fruit oil with ethanol to 5 series concentrations (1250mg/L, 2500mg/L, 5000mg/L, 10000mg/L, 20000mg/L), transferring 1 μ L of the liquid to be tested with a pipette onto the surface of the insect, observing the drying time of the solution, and repeating the treatment 3 times.

Maximum retention: diluting the rose hip oil with ethanol to 5 series concentrations (1250mg/L, 2500mg/L, 5000mg/L, 10000mg/L, 20000mg/L), and cutting certain amount of Magnolia leaf. At the same position of the blade, the blade was cut into two pieces 2 cm long and 4 cm wide with a scalpel. The blade was held with tweezers, weighed (1/10000) (W) on an electronic balance, and then held vertically in the liquid for 10s with tweezers. The leaves were quickly pulled out of the liquid surface and suspended vertically. When the drop no longer flowed, it was weighed (W1) and the maximum leaf retention R was calculated, each treatment being repeated 3 times, the formula for R being as follows:

R＝(W₁-W)/2S (S is leaf area)

Expanding the diameter: diluting the Rosa rugosa Thunb fruit oil with ethanol to 5 series concentrations (1250mg/L, 2500mg/L, 5000mg/L, 10000mg/L, 20000mg/L), transferring 1 μ L of the solution to be measured with a pipette, placing on a clean glass slide, measuring the maximum and minimum diameters of the solution, and repeating the treatment for 3 times.

The data shown in Table 5 are obtained, Table 5 being as follows:

TABLE 5 physical Properties of the solution after treatment with Rose hip oil

As shown in table 5, the results showed that as the concentration of rosehip oil increased, the drying time and surface tension of the drug solution decreased, and the spreading diameter and maximum retention of the drug solution gradually increased. The results show that rose hip oil can shorten drying time, reduce surface tension, and increase expanded diameter and maximum retention. The reduction of the surface tension enhances the adhesive force of the liquid medicine, improves the adhesive force of the liquid medicine to the wax secretion and the leaves of the Egyptian icerya, is beneficial to the use of chemical solution, and improves the prevention and treatment effect. The increase of the expanded diameter is beneficial to the diffusion of the liquid medicine on the surface of the Erythrophlomis gracilis, the effective components can better contact with insects, and the prevention and treatment effect of the matrine is improved. The maximum retention improves the adhesion of the liquid medicine on the surface of the blade, and reduces the waste caused by the sliding of the liquid medicine in the use process. When the spraying effect is poor, the control effect is generally improved by increasing the concentration of the medicament. This not only pollutes the environment, but also causes damage to non-target organisms. The rose fruit oil is added, so that the efficiency of the liquid medicine can be greatly improved. Can reduce the use of pesticides and improve the control effect. The method is environment-friendly to the maximum extent, and promotes the development of chemical pest control to the safe direction.

Conclusion

In conclusion, the rose hip oil has a good dissolving effect on wax secretion of the Egyptian icerya, has a good synergistic effect on contact killing of the Egyptian icerya when being combined with the matrine, increases penetration of the matrine on the wax secretion of the Egyptian icerya, and is probably one of the reasons for the synergy of the rose hip oil on the matrine. The rose fruit oil can also well improve the physical properties of the liquid medicine and improve the utilization rate. The pesticide dosage is reduced, the pesticide residue is reduced, the environmental pressure is relieved, the generation of the drug resistance of pests can be effectively delayed, and the prevention and control cost is reduced.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. An application of rose hip oil in preventing and treating the wax secretion of scale insects is disclosed.

2. The use of rose hip oil for dissolving wax secretions in preventing and treating scale insects according to claim 1, wherein the wax secretions comprise: the scale insect is Egyptian icerya.

3. An insecticidal formulation characterized by: comprises rose oil and pesticide.

4. The pesticidal formulation of claim 3, wherein: the pesticide is matrine.

5. The pesticidal formulation of claim 4, wherein: the mass ratio of the matrine to the rose hip oil is 1: 10-1: 500.

6. the pesticidal formulation of claim 4, wherein: the mass ratio of the matrine to the rose hip oil is 1: 50.

7. the application of a pesticide preparation is characterized in that: use of the pesticidal agent according to any one of claims 3 to 6 for controlling coccid pests.

8. A preparation method of a pesticide preparation is characterized by comprising the following steps: the preparation of the pesticidal formulation of any one of claims 3 to 6, comprising the step of mixing the rose-hip oil with the pesticidal agent.