CN113335731A

CN113335731A - Method and kit for detecting specific genes of aspergillus fumigatus and pneumocystis

Info

Publication number: CN113335731A
Application number: CN202110757775.3A
Authority: CN
Inventors: 倪剑锋; 翁毅; 史俊颖; 杨实; 刘春燕; 孙莎莎; 童惠姗; 邹昭敏
Original assignee: GENEINN BIOTECHNOLOGY (NINGBO) CO Ltd
Current assignee: GENEINN BIOTECHNOLOGY (NINGBO) CO Ltd
Priority date: 2021-07-05
Filing date: 2021-07-05
Publication date: 2021-09-03
Anticipated expiration: 2041-07-05
Also published as: CN113335731B

Abstract

The invention relates to the technical field of biological monitoring, and particularly discloses a method and a kit for detecting specific genes of aspergillus fumigatus and pneumocystis; the reagent box comprises a reagent box body and a reagent box cover, wherein the reagent box cover is rotatably connected to an opening at the upper end of the reagent box body, a first partition plate which is vertically arranged is arranged inside the reagent box body, and the first partition plate divides the inside of the reagent box body into a sampling container placing area and an auxiliary tool placing area; according to the kit disclosed by the invention, the inside of the kit is divided into regions through the plurality of partition plates, so that corresponding appliances and liquid medicine can be conveniently and quickly selected during PCR gene detection, the detection process of specific genes of aspergillus fumigatus and pneumocystis is quicker and more convenient, meanwhile, the sampling needle is more conveniently taken through the structural design of the needle storage box body, the structural design is novel, and the using effect is excellent.

Description

Method and kit for detecting specific genes of aspergillus fumigatus and pneumocystis

Technical Field

The invention relates to the technical field of biological monitoring, and particularly discloses a method and a kit for detecting specific genes of aspergillus fumigatus and pneumocystis.

Background

Aspergillus fumigatus is a conditional pathogenic fungus widely existing in natural environment, and the produced gas is transmitted to respiratory tract terminals and causes diseases related to Aspergillus sensitization. Pneumocystis generally refers to pneumocystis disease, which is a chronic mycosis of the lung caused by schericornia and one of the common deep mycoses. The most common approach for gene monitoring of both of the above fungi that cause lung disease is the detection of specific genes by PCR kits. PCR is a biological polymerase chain reaction, is a biological technology for amplifying and amplifying specific DNA fragments, and has an extremely important significance in clinical rapid judgment of bacterial infectious diseases and the like.

Present PCR detect reagent box function is comparatively single, can only detect one or two kinds of samples to can not save the articles for use relevant with the testing process, its reagent box is accomodate the back to the sample utensil and is aroused easily in the transportation and rock moreover, leads to the sample to reveal.

The utility model with the application number of 201821019348.5 discloses a novel PCR detection kit, which comprises a kit body and a kit cover, wherein the kit cover comprises a cardboard, a dyeing contrast chart and a hasp, the kit body comprises a storage box, a needle storage box, a container box, a clapboard A and a clapboard B, the storage box comprises a placing box, a second liner and a cover plate, both sides of one end of the cover plate are provided with cylindrical plugs, the plugs are embedded in round holes on the storage box, the container box and the needle storage box are separated by the clapboard B, the container box is internally provided with a first liner, eight placing holes are arranged in the first liner, the lower side of each placing hole is also pasted with a label A, a drawer is arranged in the needle storage box, the upper end of the drawer is embedded with a magnetic sheet B, one end which is contacted with an upper port of the drawer is internally provided with a magnetic block B, both sides of the drawer are provided with sliding grooves, the sliding grooves are connected with cams, and a convex ring is arranged on, the manifold characteristics of function, but put in the recess that stores up the needle box at actual testing in-process sampling needle, because the thinner difficult groove of following of sampling needle takes out fast, do not have cushioning effect when still PCR detect reagent box preserves the sample test tube in addition, can't carry out the storage of corresponding temperature according to the actual demand to the sample of gathering. Therefore, aiming at the defects of the existing PCR detection kit, the method and the kit for detecting the specific genes of aspergillus fumigatus and pneumocystis are designed, wherein the sampling needle is convenient to take, and the collected sample test tube can be buffered.

Disclosure of Invention

The invention aims to design a method and a kit for detecting specific genes of aspergillus fumigatus and pneumocystis, which are convenient for taking a sampling needle and can buffer a collected sample test tube, aiming at the defects of the existing PCR detection kit.

The invention is realized by the following technical scheme:

a kit for detecting specific genes of aspergillus fumigatus and pneumocystis comprises a reagent box body and a reagent box cover, wherein the reagent box cover is rotatably connected to an opening at the upper end of the reagent box body;

the bottom of the inner cavity of the sampling container placing area is provided with a horizontally arranged second clapboard, the lower end of the inner cavity of the sampling container placing area is divided into a sampling needle placing area by the second clapboard, the front side surface of the reagent box body positioned at the position of the sampling needle placing area is provided with a strip drawing opening, a needle storage box body for placing a sampling needle is arranged in the strip drawing opening, the upper surface of the needle storage box body is provided with a plurality of transverse strip accommodating grooves at intervals, the strip accommodating grooves are arranged on the upper surface of the needle storage box body at equal intervals and in parallel, each strip accommodating groove is provided with a needle body mounting strip, the upper surface of the needle body mounting strip is provided with a sampling needle placing clamping groove, the outer end of the sampling needle placing clamping groove is sealed and the inner end is opened, the upper surface of the needle storage box body positioned at the inner side of each strip accommodating groove is provided with a needle head clamping groove connected with the sampling needle placing clamping groove, the needle body mounting bar is characterized in that the front side and the rear side of the middle section of the needle body mounting bar are connected with protruding shafts, the needle body mounting bar is rotatably connected into a strip-shaped containing groove through the protruding shafts, the lower surface of the inner end of the needle body mounting bar is connected with a first spring, the lower end of the first spring is connected with the bottom wall of the strip-shaped containing groove, the lower surface of the front end of the needle body mounting bar is connected with an L-shaped pressing strip, an inner groove for containing the L-shaped pressing strip is formed in the front of the strip-shaped containing groove, an inserting column extending into the inner groove is inserted into the upper surface of a needle storage box body on the front side of each strip-shaped containing groove, the lower end of the inserting column is abutted against the L-shaped pressing strip, the top end of the inserting column is connected with a pressing block, and a second spring is sleeved on the inserting column between the pressing block and the upper surface of the needle storage box body;

a test tube socket is arranged in a sampling container placing area above the second partition plate, a plurality of test tube jacks are arranged on the test tube socket in a rectangular array manner, a test tube fixing partition plate is arranged in the sampling container placing area above the test tube socket, test tube sockets corresponding to the test tube jacks are arranged on the test tube fixing partition plate, a plurality of third springs are connected to the left end and the right end of the lower surface of the test tube fixing partition plate, horizontal supporting strips are connected to the lower ends of the third springs, vertically arranged elastic strips are connected to the lower surfaces of the test tube fixing partition plates on the front side and the rear side of each horizontal supporting strip, a right-angled trapezoid-shaped limiting insertion block is connected to the lower end of each elastic strip, horizontal fixing strips are fixedly connected to the inner wall of the outer end of the sampling container placing area above the test tube socket and the first partition plate, and each horizontal fixing strip is arranged under the corresponding horizontal supporting strip, the front end and the rear end of each horizontal fixing strip are provided with jacks capable of being inserted into the limiting insertion blocks;

the inside of reagent lid all is provided with dyeing contrast hardboard, the lower extreme of dyeing contrast hardboard is connected with horizontal bull stick, dyeing contrast hardboard passes through horizontal bull stick and rotates the inside of connecting at the reagent lid, still be provided with the dyeing contrast picture on the dyeing contrast hardboard.

As a further arrangement of the scheme, the reagent box body and the reagent box cover are rotatably connected through a hinge, and a buckle assembly is further arranged between the reagent box body and the reagent box cover.

As a further arrangement of the scheme, the front side face of the reagent box body is fixedly connected with a U-shaped rotating seat, and a first handle is rotatably connected in the U-shaped rotating seat.

As a further arrangement of the above scheme, a second handle is connected to the outer end face of the needle storage box body.

As a further arrangement of the above scheme, the inner end face of the needle storage box body is connected with a first magnetic stripe, the inner wall of the sampling needle placing area is connected with a second magnetic stripe corresponding to the first magnetic stripe, and the first magnetic stripe and the second magnetic stripe are arranged in a magnetic opposite manner.

As a further arrangement of the scheme, the upper surface of the needle storage box body positioned at the outer side of each strip-shaped accommodating groove is provided with a first label sticker.

As the further setting of above-mentioned scheme, be located every the test tube fixed stop upper surface of test tube socket side all is provided with the second label subsides.

As the further setting of above-mentioned scheme, every all be provided with the rubber circle in the test tube socket.

As the further setting of above-mentioned scheme, be located be provided with resistance heating piece in the test tube socket of test tube jack below, appurtenance places district inner chamber lower extreme and is provided with third insulating barrier, be provided with the battery that is used for resistance heating piece power supply in the inner chamber of third insulating barrier below.

A detection method using the kit comprises the following steps:

1) opening the reagent box cover, and taking out the injector from the auxiliary tool placing area;

2) using dNTP reaction solution, a primer probe, Taq polymerase and non-nucleic acid water in a test tube container taken by different injectors, and simultaneously taking a treated sample by using the injectors;

3) injecting the sample into a reaction test tube, injecting the used dNTP reaction solution, a primer probe, Taq polymerase and non-nucleic acid water into the reaction test tube, and heating to 90-96 ℃ for reaction for a period of time;

4) and after the reaction is finished, dipping a small amount of reaction liquid by using a sampling needle in the needle storage box body, directly spotting the reaction liquid on agar gel test paper, performing electrophoresis for 20min at a voltage of 120V, performing EB (electron beam) dyeing observation, comparing the dyeing run-through with a dyeing contrast chart to obtain a conclusion, and recording the conclusion on the basis.

Compared with the prior art, the invention has the following advantages:

1) the kit disclosed by the invention divides the interior of the kit into regions through the plurality of partition plates, and can conveniently and quickly select corresponding appliances and liquid medicine during PCR gene detection, so that the process of detecting the specific genes of aspergillus fumigatus and pneumocystis is quicker and more convenient.

2) According to the invention, the needle storage box body is provided with the plurality of strip-shaped accommodating grooves, the needle body mounting strip similar to a lever is rotatably connected in each strip-shaped accommodating groove, the tail end of the sampling needle is clamped into the sampling needle placing clamping groove when the sampling needle is placed, the needle head is placed in the needle head clamping groove, when the sampling needle with the corresponding specification is used for sampling after the reaction is finished, the pressing block can be directly pressed down, then the inner end of the needle body mounting strip is tilted through the abutting action, the needle head of the sampling needle is rotated out of the needle head clamping groove after the needle body mounting strip is tilted, a worker can directly hold the needle head to pull out the needle head from the sampling needle placing clamping groove, the whole structure design enables the sampling needle to be more convenient to take, the structure design is novel, and the using effect is excellent.

3) The lower end of a test tube container is inserted into a test tube jack on a test tube socket, and the upper end of the test tube container is inserted into a test tube jack of a test tube fixing partition plate, so that each test tube container can be clamped and fixed, and the test tube container can shake in the kit in the carrying and transporting process; in addition, due to the arrangement of the third spring, the horizontal supporting strip and the horizontal fixing strip, the test tube inserting device can play a certain buffering role on the inserted test tube, can effectively prevent the damage of the inner test tube container when the test tube container is impacted, and is higher in safety performance.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic perspective view of the present invention when deployed;

FIG. 2 is a schematic perspective view of the present invention when closed;

FIG. 3 is a schematic view of the internal plan structure of the present invention;

FIG. 4 is a schematic perspective view of the needle storage case of the present invention;

FIG. 5 is a schematic perspective view of the needle body mounting bar, the pressing bar and the first spring of the present invention;

FIG. 6 is a schematic view of the inner planar structure of the needle body mounting bar of the present invention;

FIG. 7 is an enlarged view of the structure of FIG. 7 at B in accordance with the present invention;

FIG. 8 is a schematic view of a first-angle three-dimensional structure of a test tube fixing partition plate, an elastic strip and the like in the present invention;

FIG. 9 is a schematic view of a second-angle three-dimensional structure of the test tube fixing partition plate, the elastic strip and the like in the present invention;

FIG. 10 is an enlarged view of the structure of FIG. 2 at A according to the present invention;

FIG. 11 is a flow chart of the present invention using the kit for monitoring.

Wherein:

1-reagent box body, 101-reagent box body, 102-second clapboard, 103-strip-shaped drawing opening, 104-test tube socket, 1041-test tube jack, 105-test tube fixing clapboard, 1051-test tube jack, 106-third spring, 107-horizontal supporting strip, 108-elastic strip, 109-limit insert block, 110-horizontal fixing strip, 111-U-shaped rotating seat, 112-first handle, 113-second label sticker, 114-rubber ring, 115-resistance heating block, 116-third insulating clapboard and 117-storage battery;

2-reagent box cover, 201-dyed contrast cardboard, 202-horizontal rotating rod, 203-dyed contrast picture;

3-needle storage box body, 301-strip-shaped accommodating groove, 3011-needle head clamping groove, 302-needle body mounting strip, 3021-sampling needle placing clamping groove, 303-first spring, 304-pressing strip, 305-inner groove, 306-inserting column, 307-pressing block, 308-second spring, 309-second handle, 310-first magnetic strip, 311-second magnetic strip, 312-first label sticker;

100-a sampling container placing area, 200-an auxiliary tool placing area and 300-a sampling needle placing area.

Detailed Description

In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It should be understood that the data so used may be interchanged under appropriate circumstances such that embodiments of the application described herein may be used. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover non-exclusive inclusions, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The following will describe the kit for detecting specific genes of Aspergillus fumigatus and pneumocystis disclosed in the present application in detail with reference to the accompanying drawings 1-10 and examples.

Example 1

This embodiment 1 discloses a detect kit of aspergillus fumigatus and pneumocystis specific gene, refer to fig. 1, fig. 2 and fig. 3, its main part includes reagent box body 1 and reagent lid 2, and reagent lid 2 rotates through the hinge and connects at the upper end opening part of reagent box body 1 to still be provided with buckle subassembly (not drawn in the figure) and between reagent box body 1 and reagent lid 2, can lock closed reagent box body 1 and reagent lid 2 through the buckle subassembly that sets up. Meanwhile, in order to facilitate carrying of the whole reagent kit, a U-shaped rotating seat 111 is fixedly connected to the front side face of the reagent kit body 1, and a first handle 112 is rotatably connected to the U-shaped rotating seat 111.

Referring to fig. 3, a first partition plate 101 is vertically disposed inside the reagent cartridge body 1, the first partition plate 101 divides the inside of the reagent cartridge body 1 into a sampling vessel placing area 100 and an auxiliary tool placing area 200, and a plurality of syringes for taking a liquid are placed in the auxiliary tool placing area 200. Meanwhile, a second partition plate 102 which is horizontally arranged is arranged at the bottom of the inner cavity of the sampling container placing area 100, and the second partition plate 102 divides the lower end of the inner cavity of the sampling container placing area 100 into a sampling needle placing area 300. The front side surface of the reagent box body 1 positioned at the position of the sampling needle placing area 300 is provided with a strip-shaped drawing opening 103, and a needle storage box body 3 used for placing a sampling needle is arranged in the strip-shaped drawing opening 301.

Referring to fig. 4, 5 and 6, a plurality of transverse strip-shaped receiving grooves 301 are formed on the upper surface of the needle storage box body 3 at intervals, and the strip-shaped receiving grooves 301 are arranged on the upper surface of the needle storage box body 3 at equal intervals and in parallel. All be provided with needle body mounting bar 302 in groove 301 is accomodate to every bar, the upper surface of needle body mounting bar 302 has been seted up the sampling needle and has been placed draw-in groove 3021 to the outer end that draw-in groove 3021 was placed to the sampling needle seals the setting, inner opening sets up, is seted up the syringe needle draw-in groove 3011 that the draw-in groove 3021 linked up mutually with the sampling needle is placed to the upper surface that stores up needle box 3 that lies in every bar and accomodate the groove 301 inboard. When the sampling needle is placed in the needle storage box body 3, the needle end is placed in the sampling needle placing clamping groove 3021, and the needle head is placed in the needle head clamping groove 3011.

The side all is connected with protruding axle 3022 around the interlude of needle body mounting bar 302, and needle body mounting bar 302 rotates through protruding axle 3022 to be connected in the groove 301 is accomodate to the bar to be connected with first spring 303 at the inner lower surface of needle body mounting bar 302, the lower extreme of first spring 303 is connected with the bottom wall of the groove 301 is accomodate to the bar. Referring to fig. 7, an L-shaped bead 304 is attached to the lower surface of the front end of the needle body mounting bar 302, and an inner groove 305 for accommodating the L-shaped bead 304 is provided in front of the bar-shaped receiving groove 301. An insertion column 306 extending into the inner groove 305 is inserted into the upper surface of the needle storage box body 3 positioned at the front side of each strip-shaped accommodating groove 301, the lower end of the insertion column 306 is abutted to the L-shaped pressing strip 304, the top end of the insertion column 306 is connected with a pressing block 307, and a second spring 308 is sleeved on the insertion column 306 positioned between the pressing block 307 and the upper surface of the needle storage box body 3. When taking a sample and sampling a reaction liquid, only the pressing block 307 corresponding to the needle body mounting bar 302 needs to be pressed down, the pressing bar 304 is pressed down through the inserting column 306, the inner end of the whole needle body mounting bar 302 is tilted through the lever action, and then the needle head of the sampling needle is taken out from the sampling needle placing clamping groove 3021 at the tilting position. In addition, a second handle 309 is connected to the outer end surface of the needle magazine 3 in order to facilitate the entire needle magazine 3 to be pulled out from the strip-shaped pull-out opening 103.

Referring to fig. 3, 8 and 9, a plurality of test tube sockets 1041 are formed in a rectangular array on the test tube sockets 104 in the sampling vessel placement area 100 above the second partition plate 102. A test tube fixing partition plate 105 is arranged in the sampling container placing area 100 above the test tube socket 104, a test tube socket 105 corresponding to each test tube socket 1041 is arranged on the test tube fixing partition plate 105, and a rubber ring 114 is arranged in each test tube socket 1051. The left end and the right end of the lower surface of the test tube fixing partition plate 105 are both connected with a plurality of third springs 106, and the lower ends of the third springs 106 are both connected with horizontal supporting strips 107. The test tube fixed partition 105 lower surface that is located every level and holds in the palm strip 107 front and back both sides all is connected with the elastic strip 108 of vertical setting, and the lower extreme of every elastic strip 108 is connected with the spacing inserted block 109 of right angle trapezoidal form. Equal fixedly connected with horizontal fixed strip 110 on being located the sample container of test tube socket 104 top and placing district 100 outer end inner wall and first baffle 101 to every horizontal fixed strip 110 all sets up under corresponding horizontal support strip 107, and the jack that can insert spacing inserted block 109 (can refer to fig. 10) has all been seted up at both ends around every horizontal fixed strip 110. When placing the test tube container in sample container placing area 100 directly insert the test tube through test tube socket 1051 and fix in test tube jack 1041 can, then use the stopcock to seal the test tube open end. The above-mentioned elastic strip 108 of this embodiment, spacing inserted block 109 and horizontal fixed strip 110 set up can be convenient for take off test tube fixed partition 105 is whole, and the effect between third spring 106, horizontal support strip 107 and the horizontal fixed strip 110 can play certain cushioning effect to the test tube after inserting.

Referring to fig. 2 and 3, a dyeing contrast cardboard 201 is arranged inside the reagent box cover 2, a horizontal rotating rod 202 is connected to the lower end of the dyeing contrast cardboard 201, the dyeing contrast cardboard 201 is rotatably connected inside the reagent box cover 2 through the horizontal rotating rod 202, and a dyeing contrast chart 203 is further arranged on the dyeing contrast cardboard 201.

Example 2

Embodiment 2 discloses a kit for detecting specific genes of aspergillus fumigatus and pneumocystis based on improvement of embodiment 1, the same as embodiment 1 is not described again, the difference refers to fig. 3 and fig. 4, the embodiment further comprises a first magnetic stripe 310 connected to the inner end face of the needle storage box body 3, a second magnetic stripe 311 corresponding to the first magnetic stripe 310 is connected to the inner wall of the sampling needle placement area 300, the first magnetic stripe 310 and the second magnetic stripe 311 are arranged in opposite magnetic directions, and the needle storage box body 3 can be stably fixed in the sampling needle placement area 300 and is not easy to slip out through mutual adsorption between the two magnetic stripes.

Referring to fig. 4 and 8, the present embodiment 2 further includes a first label 312 disposed on the upper surface of the needle storage box 3 outside each strip-shaped receiving groove 301. A second label 113 is also arranged on the upper surface of the test tube fixing partition plate 105 beside each test tube socket 1051. Through first label subsides and second label subsides can make clear and definite marks to test tube container built-in liquid and sampling needle specification, make things convenient for follow-up solution and the sampling needle who uses correspondence between according to the label subsides.

Finally, referring to fig. 3, in this embodiment 2, a resistance heating block 115 is further disposed in the test tube socket 104 located below the test tube jack 1041, a third insulating partition 116 is disposed at the lower end of the inner cavity of the auxiliary tool placing area 200, a storage battery 117 for supplying power to the resistance heating block 115 is disposed in the inner cavity below the third insulating partition 116, and the temperature of the solution in the test tube container can be preheated through the setting of the resistance heating block 115, so that the solution can be taken conveniently and then has a certain temperature, and the time for subsequent reaction heating is reduced.

In addition, the invention also discloses a method for detecting the specific genes of aspergillus fumigatus and pneumocystis by using the kit disclosed in the embodiment 2 (refer to the attached figure 11), which comprises the following steps:

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a detect kit of aspergillus fumigatus and pneumocystis specific gene, includes reagent box body (1) and reagent lid (2), reagent lid (2) rotate to be connected at the upper end opening part of reagent box body (1), its characterized in that, the inside of reagent box body (1) is provided with first baffle (101) of vertical arrangement, first baffle (101) divide into the inside of reagent box body (1) that the sample vessel placed district (100) and appurtenance and place district (200).

2. The kit for detecting the specific genes of aspergillus fumigatus and pneumocystis according to claim 1, wherein a second partition board (102) horizontally arranged is arranged at the bottom of the inner cavity of the sampling container placing area (100), the lower end of the inner cavity of the sampling container placing area (100) is divided into a sampling needle placing area (300) by the second partition board (102), a strip-shaped drawing opening (103) is arranged at the front side surface of the reagent box body (1) at the position of the sampling needle placing area (300), a needle storage box body (3) for placing a sampling needle is arranged in the strip-shaped drawing opening (301), a plurality of transverse strip-shaped accommodating grooves (301) are arranged at intervals on the upper surface of the needle storage box body (3), the strip-shaped accommodating grooves (301) are arranged at equal intervals and in parallel on the upper surface of the needle storage box body (3), and a needle body mounting strip (302) is arranged in each strip-shaped accommodating groove (301), the upper surface of the needle body mounting bar (302) is provided with a sampling needle placing clamping groove (3021), the outer end of the sampling needle placing clamping groove (3021) is sealed and arranged, the inner end of the sampling needle placing clamping groove is opened, the upper surface of the needle storage box body (3) positioned at the inner side of each strip accommodating groove (301) is provided with a needle clamping groove (3011) connected with the sampling needle placing clamping groove (3021), the front side and the rear side of the middle section of the needle body mounting bar (302) are both connected with a convex shaft (3022), the needle body mounting bar (302) is rotatably connected in the strip accommodating groove (301) through the convex shaft (3022), the lower surface of the inner end of the needle body mounting bar (302) is connected with a first spring (303), the lower end of the first spring (303) is connected with the bottom wall of the strip accommodating groove (301), the lower surface of the front end of the needle body mounting bar (302) is connected with an L-shaped pressing strip (304), the front of the strip accommodating groove (301) is provided with an inner groove (305) for accommodating the L-shaped pressing strip (304), an inserting column (306) extending into the inner groove (305) is inserted into the upper surface of the needle storage box body (3) positioned on the front side of each strip-shaped accommodating groove (301), the lower end of the inserting column (306) is abutted to the L-shaped pressing strip (304), the top end of the inserting column (306) is connected with a pressing block (307), and a second spring (308) is sleeved on the inserting column (306) positioned between the pressing block (307) and the upper surface of the needle storage box body (3);

a test tube socket (104) is arranged in a sampling container placing area (100) above the second partition plate (102), a plurality of test tube jacks (1041) are formed in the test tube socket (104) in a rectangular array mode, a test tube fixing partition plate (105) is arranged in the sampling container placing area (100) above the test tube socket (104), test tube sockets (1051) corresponding to each test tube jack (1041) are formed in the test tube fixing partition plate (105), the left end and the right end of the lower surface of the test tube fixing partition plate (105) are connected with a plurality of third springs (106), the lower ends of the third springs (106) are connected with horizontal supporting strips (107), the lower surfaces of the test tube fixing partition plates (105) on the front side and the rear side of each horizontal supporting strip (107) are connected with vertically arranged elastic strips (108), and the lower end of each elastic strip (108) is connected with a right-angle trapezoidal limiting insert block (109), horizontal fixing strips (110) are fixedly connected to the inner wall of the outer end of the sampling container placing area (100) above the test tube socket (104) and the first partition plate (101), each horizontal fixing strip (110) is arranged right below the corresponding horizontal supporting strip (107), and jacks capable of being inserted into the limiting insertion blocks (109) are formed in the front end and the rear end of each horizontal fixing strip (110);

a dyeing contrast hard board (201) is arranged inside the reagent box cover (2), the lower end of the dyeing contrast hard board (201) is connected with a horizontal rotating rod (202), the dyeing contrast hard board (201) is rotatably connected inside the reagent box cover (2) through the horizontal rotating rod (202), and a dyeing contrast picture (203) is further arranged on the dyeing contrast hard board (201); the reagent box body (1) and the reagent box cover (2) are connected in a rotating mode through a hinge, and a buckle assembly is further arranged between the reagent box body (1) and the reagent box cover (2).

3. The kit for detecting specific genes of aspergillus fumigatus and pneumocystis according to claim 2, characterized in that a U-shaped rotating seat (111) is fixedly connected to the front side surface of the reagent box body (1), and a first handle (112) is rotatably connected to the U-shaped rotating seat (111).

4. The kit for detecting specific genes of aspergillus fumigatus and pneumocystis according to claim 2, wherein the outer end surface of the needle storage box body (3) is connected with a second handle (309).

5. The kit for detecting specific genes of aspergillus fumigatus and pneumocystis according to claim 2, wherein the inner end surface of the needle storage box body (3) is connected with a first magnetic stripe (310), the inner wall of the sampling needle placing area (300) is connected with a second magnetic stripe (311) corresponding to the first magnetic stripe (310), and the first magnetic stripe (310) and the second magnetic stripe (311) are arranged in a magnetic opposite manner.

6. The kit for detecting specific genes of aspergillus fumigatus and pneumocystis according to claim 2, wherein the upper surface of the needle storage box body (3) positioned at the outer side of each strip-shaped storage groove (301) is provided with a first label sticker (312).

7. The kit for detecting specific genes of aspergillus fumigatus and pneumocystis according to claim 2, wherein a second label (113) is disposed on the upper surface of the tube fixing partition (105) beside each tube insertion opening (1051).

8. The kit for detecting specific genes of aspergillus fumigatus and pneumocystis according to claim 2, wherein a rubber ring (114) is arranged in each test tube socket (1051).

9. The kit for detecting the specific genes of aspergillus fumigatus and pneumocystis according to claim 2, wherein a resistance heating block (115) is arranged in the test tube socket (104) below the test tube jack (1041), a third insulating partition plate (116) is arranged at the lower end of the inner cavity of the auxiliary tool placing area (200), and a storage battery (117) for supplying power to the resistance heating block (115) is arranged in the inner cavity below the third insulating partition plate (116).

10. An assay method using the kit according to any one of claims 2 to 9, comprising the steps of: