CN112707014A

CN112707014A - Tumor detection kit

Info

Publication number: CN112707014A
Application number: CN202011510020.5A
Authority: CN
Inventors: 吴培; 张云; 赵爱兵
Original assignee: Anhui Baioujing Medical Technology Co ltd
Current assignee: Anhui Baioujing Medical Technology Co ltd
Priority date: 2020-12-18
Filing date: 2020-12-18
Publication date: 2021-04-27

Abstract

The invention provides a tumor detection kit, which comprises a box body and a box cover, wherein a freezing groove, a bearing plate arranged above the freezing groove, a placing frame and a lifting mechanism for driving the bearing plate to move up and down are arranged in the box body, the placing frame is provided with a test tube positioning through hole, a first flexible pad is arranged on the inner wall of the positioning through hole, and a positioning clamping structure for positioning the bottom end of a test tube is arranged on the bearing plate. Through the setting of elevating system, can assist taking out of reagent pipe, make things convenient for taking of reagent pipe. Through the setting of location joint structure, the reagent pipe of being convenient for puts into the location behind the kit, avoids rocking. The flexible pad that the via hole inner wall set up is fixed a position, makes reagent pipe have the buffering contact effect, avoids producing the contact damage.

Description

Tumor detection kit

Technical Field

The invention relates to the technical field of gene detection, in particular to a tumor detection kit.

Background

The kit is a box for containing chemical reagents for detecting chemical components, drug residues, virus species and the like. Generally, hospitals and pharmaceutical enterprises use the sampling method, and in clinical medical activities and biomedical scientific research and experiment works, a plurality of samples with biological and chemical toxicity are required to be sampled.

The tumor marker is also called tumor marker, and refers to a substance which is characterized in malignant tumor cells, or is produced by malignant tumor cells in an abnormal way, or is produced by the stimulation response of a host to the tumor, and can reflect the occurrence and development of the tumor and monitor the response of the tumor to treatment. Tumor markers are present in tissues, body fluids and excretions of tumor patients and can be detected immunologically, biologically and chemically. Tumor markers are often stored in kits.

The existing tumor marker detection kit is inconvenient for taking a reagent, the tumor marker detection reagent is stored in the kit and is often inconvenient to take, so that the tumor marker detection reagent is damaged, and the existing tumor marker detection kit with the reagent convenient to take is urgently needed to solve the problems.

In addition, when the kit is used, a plurality of reagent bottles cannot be used orderly, so that the kit is inconvenient to use and study, and the kit cannot give consideration to clinically common lung cancer and all common tumors, so that the flexibility of the kit in use is poor.

Disclosure of Invention

The invention aims to solve the technical problems in the prior art. Therefore, the invention provides a tumor detection kit, which aims to facilitate the taking of a reagent tube and has a buffering and positioning effect on the reagent tube.

Based on the above purpose, the invention provides a tumor detection kit, which comprises a box body and a box cover, wherein a freezing groove, a bearing plate arranged above the freezing groove, a placing frame and a lifting mechanism for driving the bearing plate to move up and down are arranged in the box body, the placing frame is provided with a test tube positioning through hole, the inner wall of the positioning through hole is provided with a first flexible pad, and the bearing plate is provided with a positioning clamping structure for positioning the bottom end of a test tube.

The location joint structure is including the positioning channel who locates the loading board up end, positioning channel and test tube bottom joint, and positioning channel and test tube location via hole are corresponding from top to bottom.

Elevating system is including the screw rod lifting unit who locates the loading board both ends, screw rod lifting unit includes turning block, screw rod, nut piece and locates the thread bush at nut piece middle part, the top of screw rod is passed the rack and is connected with the turning block of locating the board up end of placing, the bottom of screw rod is passed the thread bush and is connected with the up end rotation of freezing groove.

An elastic buffer mechanism is arranged between the bearing plate and the freezing groove.

The elastic buffer mechanism is a connecting spring.

The inner wall of the box body is provided with a sponge layer.

And an anti-collision rubber layer is arranged between the sponge layer and the inner wall of the box body.

The inside refrigerating fluid that is equipped with of freezing groove, the freezing groove is made by nylon resistance to compression leak protection complex film.

The refrigerant fluid is a gelatinous mixture, and the gelatinous mixture contains sodium polyacrylate.

Test tube location via hole and location joint structure are a plurality of, and test tube location via hole and location joint mechanism one-to-one, install first mixed liquid reagent bottle in a plurality of test tube location via holes respectively, the mixed liquid reagent bottle of second, PCR reaction solution reagent bottle and joint reaction solution reagent bottle, fragmentation/terminal repair buffer solution reagent bottle, whole genome fragmentation/terminal repair reaction reagent bottle, DNA connects buffer solution reagent bottle, DNA ligase reagent bottle, human genome DNA reagent bottle, hybridization buffer solution reagent bottle, hybridization buffering reinforcing liquid reagent bottle, confining liquid reagent bottle, magnetic bead washing liquid reagent bottle, first washing liquid reagent bottle, second washing liquid reagent bottle, third washing liquid reagent bottle, powerful washing liquid reagent bottle, streptavidin magnetic bead reagent bottle, library amplification primer reagent bottle, MgCl₂The device comprises an ion reagent bottle, an ultrapure water reagent bottle, a first probe reagent bottle and a second probe reagent bottle.

The invention has the beneficial effects that: according to the invention, through the arrangement of the lifting mechanism, the reagent tube can be taken out in an auxiliary manner, so that the reagent tube is convenient to take. Through the setting of location joint structure, the reagent pipe of being convenient for puts into the location behind the kit, avoids rocking. The flexible pad that the via hole inner wall set up is fixed a position, makes reagent pipe have the buffering contact effect, avoids producing the contact damage. The elastic buffer mechanism arranged between the bearing plate and the freezing groove can further play a role in buffering, and meanwhile, the bearing plate on the upper portion is stably supported. Corresponding reagent pipe is placed respectively to a plurality of reagent pipe location via holes for numerous reagent bottles use neatly orderly, thereby make the use of this kit and study simple accurate, the user can select the probe to the lung cancer and the probe to multiple tumour as required simultaneously, makes this kit can compromise common lung cancer and all common tumours clinically, thereby flexibility when making this kit use is stronger.

Drawings

In order to more clearly illustrate the embodiments of the present disclosure or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only one or more embodiments of the present disclosure, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic structural view of example 1 of the present invention;

fig. 2 is a schematic structural view of a rack in embodiment 1 of the present invention;

FIG. 3 is a schematic structural view of example 2 of the present invention;

fig. 4 is a schematic view of a partial structure of a placement frame in embodiment 3 of the present invention.

Labeled as:

1. a box body; 2. a box cover; 3. a freezing tank; 4. a carrier plate; 5. placing a rack; 6. a first flexible mat; 7. positioning the clamping groove; 8. rotating the block; 9. a screw; 10. a nut block; 11. a first sponge layer; 12. a second sponge layer; 13. a connecting spring; 14. mounting grooves; 15. a test strip; 16. a first reagent bottle section; 17. a second reagent bottle section; 18. a third reagent bottle part; 19. a fourth reagent bottle part.

Detailed Description

To make the objects, technical solutions and advantages of the present disclosure more apparent, the present disclosure is further described in detail below with reference to specific embodiments.

It should be noted that technical terms or scientific terms used in the embodiments of the present specification should have a general meaning as understood by those having ordinary skill in the art to which the present disclosure belongs, unless otherwise defined. The use of "first," "second," and similar terms in the embodiments of the specification is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.

As shown in fig. 1 to 3, a tumor detection kit comprises a box body 1 and a box cover 2, wherein a freezing groove 3, a bearing plate 4 and a placing frame 5 which are arranged above the freezing groove 3 and a lifting mechanism for driving the bearing plate 4 to move up and down are arranged in the box body 1, the placing frame 5 is provided with a test tube positioning via hole, a first flexible pad 6 is arranged on the inner wall of the positioning via hole, and a positioning clamping structure for positioning the bottom end of a test tube is arranged on the bearing plate 4. Through the setting of elevating system, can assist taking out of reagent pipe, make things convenient for taking of reagent pipe. Through the setting of location joint structure, the reagent pipe of being convenient for puts into the location behind the kit, avoids rocking. The flexible pad that the via hole inner wall set up is fixed a position, makes reagent pipe have the buffering contact effect, avoids producing the contact damage. The following is detailed by specific examples:

example 1

As shown in fig. 1 and fig. 2, in this embodiment, the positioning and clamping structure includes a positioning slot 7 disposed on the upper end surface of the loading plate 4, the positioning slot 7 is clamped with the bottom end of the test tube, and the positioning slot 7 corresponds to the test tube positioning via hole vertically. The preferred arc draw-in groove with test tube bottom looks adaptation of positioning channel section, positioning channel section through with test tube bottom shape looks adaptation joint, the flexible contact centre gripping of the downthehole flexible pad of cooperation location cross avoids the test tube to appear making a round trip the phenomenon of rocking after the installation, avoids appearing the extrusion damage simultaneously. The first flexible pad can be a soft rubber pad.

Elevating system is including locating the 9 lifting unit of screw rod at 4 both ends of loading board, and 9 lifting unit of screw rod include turning block 8, screw rod 9, nut piece 10 and locate the thread bush at nut piece 10 middle part, and the rack 5 is passed and the turning block 8 of locating the board up end is connected on the top of screw rod 9, the bottom of screw rod 9 is passed the thread bush and is connected with the up end rotation of freezing groove 3. During the setting, the middle division of nut block is equipped with the mounting hole, and the annular side is carved with the internal thread in the mounting hole, and the annular side of screw thread cover can be equipped with the external screw thread, and external screw thread and internal thread mesh mutually, make things convenient for the thread bush to install inside the nut block. When taking tumour marker detection reagent, open the lid, then twist and move the turning block, the turning block drives the screw rod and rotates, because of thread bush and screw rod mesh mutually, the thread bush is installed inside the nut piece for the nut piece is by supreme removal down along the screw rod, and the nut piece removes and drives the loading board rebound, and the spring atress extrusion that makes progress, loading board rebound drive reagent pipe rebound, can realize taking fast of reagent pipe.

In this embodiment, be equipped with test strip mounting groove 14 and colour chip 15 on the rack, install the cellulose nitrate section on the test strip in the test strip mounting groove and locate the filter paper section and the specific antigen section that absorb water of cellulose nitrate section both sides. Wherein, the colorimetric card is used for conveniently comparing detection results; the inner chamber of test strip mounting groove bonds and has the test strip, and the top left side of test strip bonds and has the filter paper section that absorbs water, and the top middle bonding of test strip has the cellulose nitrate section, and the top right side of test strip bonds and has the specificity antigen section, and the test strip is used for bearing the filter paper section that absorbs water, cellulose nitrate and specificity antigen section, and the filter paper section that absorbs water, cellulose nitrate section and specificity antigen section are used for detecting the marker. Through set up a plurality of test strip mounting grooves and test strip on placing the board in the box body, conveniently detect a plurality of markers, through the cooperation of reagent bottle, test strip and colour comparison card, conveniently contrast the testing result, improve detection efficiency.

The inside of the freezing tank 3 is provided with refrigerating fluid, and the freezing tank 3 is made of a nylon compression-resistant leakage-proof composite film. The refrigerant fluid is a gelatinous mixture, and the component of the gelatinous mixture is sodium polyacrylate. Through being equipped with the refrigerating fluid, can avoid the condition that the damage appears because of the high temperature in the loading thing, simultaneously because the freezing groove is made by nylon resistance to compression leak protection complex film, can avoid freezing the damaged condition that leads to the refrigerating fluid in it to appear leaking of groove to be convenient for store and transport.

In order to reduce the influence of the transportation vibration on the interior of the box body, a sponge layer is arranged on the inner wall of the box body 1. Particularly, set up first sponge layer 11 in the bottom of box body, also set up corresponding sponge layer at the circumference lateral wall of box body, set up second sponge layer 12 at the inner wall of lid. Meanwhile, due to the water absorption of the sponge, a certain amount of water can be absorbed, and the phenomenon of liquid leakage in the box body is avoided.

Example 2

As shown in fig. 3, the present embodiment is different from embodiment 1 in that an elastic buffer mechanism is provided between the carrying plate 4 and the freezing tank 3. The elastic buffer mechanism is preferably a connecting spring 13. The elastic buffer mechanism arranged between the bearing plate and the freezing groove can further play a role in buffering, and meanwhile, the bearing plate on the upper portion is stably supported.

Example 3

As shown in fig. 4, the present embodiment is different from embodiment 1 in that the test tube positioning via holes and the positioning clamping structure are multiple, the test tube positioning via holes correspond to the positioning clamping mechanism one by one, and respectively include a first reagent bottle portion 16, a second reagent bottle portion 17, a third reagent bottle portion 18 and a fourth reagent bottle portion 19, and the first mixed solution reagent bottle and the second mixed solution reagent bottle are respectively installed in the test tube positioning via holesThe kit comprises a solution mixing reagent bottle, a PCR reaction solution reagent bottle, a joint reaction solution reagent bottle, a fragmentation/terminal repair buffer solution reagent bottle, a whole genome fragmentation/terminal repair reaction reagent bottle, a DNA connection buffer solution reagent bottle, a DNA ligase reagent bottle, a human genome DNA reagent bottle, a hybridization buffer solution reagent bottle, a hybridization buffer enhancement solution reagent bottle, a sealing solution reagent bottle, a magnetic bead cleaning solution reagent bottle, a first cleaning solution reagent bottle, a second cleaning solution reagent bottle, a third cleaning solution reagent bottle, a powerful cleaning solution reagent bottle, a streptavidin magnetic bead reagent bottle, a library amplification primer reagent bottle, a MgCl2 ion reagent bottle, an ultrapure water reagent bottle, a first probe reagent bottle and a second probe reagent bottle. The first mixed solution reagent bottle, the second mixed solution reagent bottle, the PCR reaction solution reagent bottle and the joint reaction solution reagent bottle are arranged at the first reagent bottle part; the fragmentation/terminal repair buffer solution reagent bottle, the whole genome fragmentation/terminal repair reaction reagent bottle, the DNA connection buffer solution reagent bottle and the DNA ligase reagent bottle are arranged at the second reagent bottle part; human genome DNA reagent bottle, hybridization buffer solution reagent bottle, hybridization buffer enhancement solution reagent bottle, sealing solution reagent bottle, magnetic bead cleaning solution reagent bottle, first cleaning solution reagent bottle, second cleaning solution reagent bottle, third cleaning solution reagent bottle, high-efficiency cleaning solution reagent bottle, streptavidin magnetic bead reagent bottle, library amplification primer reagent bottle, MgCl₂The ion reagent bottle and the ultrapure water reagent bottle are arranged in the third reagent bottle part; the first probe reagent bottle and the second probe reagent bottle are arranged on the fourth reagent bottle part. When the kit is used, a user can carry out one-to-one operation through the specification, so that a plurality of reagent bottles are used orderly, the kit is convenient and easy to use and learn, meanwhile, the user can select probes for lung cancer and probes for various tumors according to needs, the kit can take common lung cancer and all common tumors into consideration clinically, and the kit is high in flexibility when in use.

As a further improvement, an anti-collision rubber layer is arranged between the sponge layer and the inner wall of the box body 1. The sponge layer and the rubber layer on the inner layer of the box body improve the stability of the box body, buffer the impact force caused by collision and have good shock absorption effect.

Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to intimate that the scope of the disclosure, including the claims, is limited to these examples; within the idea of the present disclosure, also technical features in the above embodiments or in different embodiments may be combined, steps may be implemented in any order, and there are many other variations of the different aspects of the embodiments of the present description as described above, which are not provided in detail for the sake of brevity.

The embodiments of the present description are intended to embrace all such alternatives, modifications and variances that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalents, improvements, and the like that may be made within the spirit and principles of the embodiments described herein are intended to be included within the scope of the disclosure.

Claims

1. The utility model provides a tumor detection kit, includes box body and lid, its characterized in that, be equipped with freezing groove in the box body, locate loading board, rack and the elevating system who is used for driving loading board elevating movement of freezing groove top, the rack is equipped with test tube location via hole, location via hole inner wall is equipped with first flexible pad, be equipped with the location joint structure to the test tube bottom location on the loading board.

2. The tumor detection kit according to claim 1, wherein the positioning and clamping structure comprises a positioning slot disposed on the upper end surface of the loading plate, the positioning slot is clamped with the bottom end of the test tube, and the positioning slot corresponds to the positioning through hole of the test tube.

3. The tumor detection kit according to claim 1, wherein the lifting mechanism comprises screw lifting assemblies disposed at two ends of the loading plate, each screw lifting assembly comprises a rotating block, a screw, a nut block and a threaded sleeve disposed in the middle of the nut block, the top end of the screw penetrates through the placement frame and is connected with the rotating block disposed on the upper end face of the placement plate, and the bottom end of the screw penetrates through the threaded sleeve and is rotatably connected with the upper end face of the freezing tank.

4. The kit of claim 1, wherein an elastic buffer mechanism is disposed between the carrier plate and the freezing chamber.

5. The tumor detection kit according to claim 4, wherein the elastic buffer mechanism is a connecting spring.

6. The tumor detection kit according to claim 1, wherein a sponge layer is disposed on the inner wall of the case body.

7. The tumor detection kit according to claim 6, wherein an anti-collision rubber layer is arranged between the sponge layer and the inner wall of the box body.

8. The tumor detection kit according to claim 1, wherein the freezing tank is filled with a refrigerant fluid and is made of a nylon compression-resistant and leak-proof composite film.

9. The tumor detection kit according to claim 8, wherein the refrigerant fluid is a gel-like mixture, and the gel-like mixture comprises sodium polyacrylate.

10. The tumor detection kit according to claim 1, wherein the test tube positioning via holes and the positioning clamping structure are multiple, the test tube positioning via holes correspond to the positioning clamping mechanism one by one, and a first mixed solution reagent bottle, a second mixed solution reagent bottle, a PCR reaction solution reagent bottle, a joint reaction solution reagent bottle, a fragmentation/end repair buffer solution reagent bottle, a whole genome fragmentation/end repair reaction reagent bottle, a DNA connection buffer solution reagent bottle, a DNA ligase reagent bottle, a human genome DNA reagent bottle, a hybridization buffer solution reagent bottle, a hybridization enhancement buffer solution test bottle, are respectively installed in the test tube positioning via holesReagent bottle, sealing liquid reagent bottle, magnetic bead cleaning liquid reagent bottle, first cleaning liquid reagent bottle, second cleaning liquid reagent bottle, third cleaning liquid reagent bottle, powerful cleaning liquid reagent bottle, streptavidin magnetic bead reagent bottle, library amplification primer reagent bottle, MgCl₂The device comprises an ion reagent bottle, an ultrapure water reagent bottle, a first probe reagent bottle and a second probe reagent bottle.