CN1133275A - Znzyme transformed organic fertilizer - Google Patents
Znzyme transformed organic fertilizer Download PDFInfo
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- CN1133275A CN1133275A CN95119430A CN95119430A CN1133275A CN 1133275 A CN1133275 A CN 1133275A CN 95119430 A CN95119430 A CN 95119430A CN 95119430 A CN95119430 A CN 95119430A CN 1133275 A CN1133275 A CN 1133275A
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- bone
- znzyme
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The present invention relates to an organic fertilizer richly containing phosphorus, calcium, nitrogen and trace elements. Its preparation method is as follows: the phosphorus and calcium are dissolved out from animal bone by using high-pressure steam refluxing process, then the waste materials richly containing protein, such as animal hoof, horn, etc. are added in the obtained solution, then the above-mentioned materials are heated under the acidic condition so as to make the protein hydrolyze, and the protein is hydrolyzed into water-soluble protein, and then the aspergillus oryzae is added so as to convert the water-soluble protein into amino acid, and then the trace elements necessary for plant and human body are added, so that the invented fertilizer can be obtained.
Description
The present invention relates to a kind of fertilizer, more particularly, relate to a kind of fertilizer that assorted waste materials such as animal bone and hoof, angle, hair, feather make by enzymatic conversion and preparation method thereof that utilizes.
Existing fertilizer, for example bone meal, cake fertilizer etc. all will impose in the soil, under certain condition through spontaneous fermentation, make protein transduction turn to amino acid, then could be by plant absorbing, thereby amount of application is big, it is slow to take effect.German Patent DT2756739 discloses a kind of method of making feed and fertilizer, and this method is to utilize the usefulness acid such as processing process wastewater of animal bone, waste material and meat product or alkali to be hydrolyzed to cause protein denaturation, as feed and fertilizer.But this fertilizer, owing to albumen is not converted into amino acid, so still there is the big and slower problem that takes effect of amount of application, cooperate necessary trace element because this fertilizer is provided with in addition, thereby make the plant of local cultivation and be that the humans and animals of main food is easily suffered from certain class and lacked disorderly disease with these plants.
The objective of the invention is to provide a kind of is main raw material with the animal body waste material, processes and be combined with Znzyme transformed organic fertilizer of necessary trace element and preparation method thereof by enzymatic conversion.
The invention provides a kind of Znzyme transformed organic fertilizer, it is characterized in that, it is made by following method:
With 100 animal bone of amount part (preferred ox bone and/or pig bone) fragmentations again, broken bone is put into high press-steamed pot, feed 4-5kg/cm
2The saturation steam of (definitely) pressure keeping vexed jar the steaming more than 15 minutes under this pressure, stops logical steam, is cooled to 1kg/cm
2(definitely) is below the pressure, from jar, discharge condensation and steam bone water, so repeatedly for several times, steam bone water be as the criterion (grease that preferably will steam waterborne layer of bone is removed) with the condensation of collecting the 20-30 weight part, the pH value that will steam bone water with acid (being preferably sulfuric acid and/or phosphoric acid) transfers to 2-3.5, steaming bone water after the acid adjustment is transferred in the autoclave, is selected from one or more mixture in the hoof, angle, hair, feather of animal, keeping 1.05-1.2kg/cm toward wherein adding the 7-15 weight part
2Under (definitely) pressure heating hydrolysis 4-6 hour, cool off, take the dish out of the pot and the elimination throw out, with alkali (being preferably sodium hydroxide, yellow soda ash or lime) filtrate is neutralized to PH4-5, pastly then wherein add the 0.005-0.01 weight part and include 5 * 10
8The aspergillus oryzae of individual/above thalline of g (preservation of institute of microbiology of the Chinese Academy of Sciences, preserving number AS.3.951) culture stirs, filter, in filtrate, add each 0.05-0.1 weight part of copper sulfate, zinc sulfate, manganous sulfate, ferrous sulfate and ammonium molybdate, stir the packing finished product.
At length explain the present invention below.
Said in the present invention animal bone can be various animal bone, but wherein is preferably ox bone or pig bone.To produce the extraction efficiency of steaming bone water step in order improving, this animal bone fragmentation, its granularity should not to be had strict restriction, generally be advisable with 10-30cm.Producing the main purpose of steaming bone water is phosphorus and the calcium that extracts in the animal bone.Because therefore difficult stripping from animal bone of phosphorus and calcium adopts higher vapor pressure (4-5kg/cm
2Be equivalent to about 145 °-150 ℃).Water vapor plays a kind of backflow leaching effect in high press-steamed pot, just the phosphorus in the animal bone and calcium are extracted when it under high pressure is condensed into water and ask.After the vapour source of high press-steamed pot is closed,,, pressure approaches 1kg/cm when being reduced to owing to a jar interior water vapor condensation becomes water that pressure is reduced rapidly
2Can steam bone water from the discharging of the lower end of jar when (being normal pressure), usually will vexed repeatedly steaming and the operation of draining can collect the steaming bone water that is equivalent to animal bone weight 20-30% for 3-5 time 4.The animal bone residue that has extracted behind phosphorus and the calcium can be made into bone meal as uses such as feeds.At some grease of the steaming bone of collecting layer waterborne, this part grease can not removed, but preferably is removed, and the operation to follow-up is more favourable like this.
The used raw material of hydrolysing step is hoof, angle, hair or feather of animal or their mixture, its objective is these insoluble proteins are become soluble proteins, just nitrogen is wherein become the water miscible composition that fertilizer efficiency is arranged.Hydrolysing step should carry out under the condition of acid (PH2-3.5, preferred 2.5).The acid that is used to regulate pH value is preferably sulfuric acid and phosphoric acid, and itself has fertilizer efficiency phosphoric acid, but its price is more expensive.The chlorine root of hydrochloric acid is unfavorable to the growth of plant, so preferably need not.The temperature of hydrolysis should be between a little more than 100 ℃ to 106 ℃, so adopt 1.05-1.2kg/cm
2Vapor pressure, when temperature was higher than 107 ℃, protein was easy to decompose, thereby caused the loss of nitrogen, so should not adopt.
After the hoof of animal, angle, hair, feather etc. are transformed into water miscible albumen, still be not easy to be absorbed rapidly by plant, therefore also to be translated into very easily by the amino acid of plant absorbing with microorganism.The inventor has found that through after the long term studies a kind of microbial preparation of aspergillus oryzae by name is very effective, and this kind microorganism is in all preservations of Chinese Academy of Sciences's microbe research, and its preserving number is AS.3.951.This aspergillus oryzae preparation commodity are produced by Heilongjiang Province's biological study, and each city and other each big cities, the whole nation are all on sale in the Heilongjiang Province.
Said aspergillus oryzae can be directly used in enzymatic conversion reaction, but from considering economically, when scale operation Znzyme transformed organic fertilizer of the present invention, preferably the aspergillus oryzae bought is re-used after through three grades of enlarged culturing.In the present invention, three grades of suitable cultural methods are as follows: the first step and the second stage are cultivated and are all used conventional peptone liquid nutrient medium, and the primary condition of cultivation is PH6-7; Temperature 28-32 ℃, 48 hours time.Substratum when the third stage is cultivated is a solid medium, raw material is soya-bean cake 70% (weight)+wheat bran 30% (weight), adding water to water content is 50-60%, cook, after the cooling pH value is transferred to 6-7, inoculum size is 25-35g/100kg, culture temperature keeps 36-38 ℃, the green hair of expression mould will appear here in about 24 hours of time, but thalline reaches 500,000,000/g (5 * 10 during preferably with microscopy
8Individual/as to be as the criterion more than g).Adopting solid medium when the third stage is cultivated mainly is for the ease of transportation and storage.But when adopting solid medium, said enzymatic conversion process in carrying out the present invention and after adding aspergillus oryzae, residual throw out should being removed by filter to guarantee that acquisition is suitable for directly spraying the liquid product of usefulness.If necessary, behind the vitriol that adds various required trace elements, refilter once.Promptly can be used as the finished product then sells.
Compare with the various fertilizers of prior art, the beneficial effect of Znzyme transformed organic fertilizer of the present invention is: the fertilizer efficiency height, easy to use, instant effect, do not need any conversion can be directly by plant absorbing, do not contain any tethelin one class chemosynthesis material, therefore can not produce any bad side effect human body.And replenished trace element and organic substance by crop, improved the quality of root, stem, leaf and the fruit of crop, can replenish the various trace elements of needed by human body, HUMAN HEALTH has been highly profitable.Therefore the present invention is referred to as the fertilizer of a new generation.
The using method of Znzyme transformed organic fertilizer of the present invention is very simple, and the length of general visual crop growth period sprays in the phase in whole growth and to get final product for 2-4 time.For example, for cucumber, can respectively spray once at branch phase, flower early stage and young fruit period, every mu of consumption is 100 milliliters (stostes) when spraying, and carries out necessary dilution before spraying, and generally is preferably dilution 400-500 and carries out foliage-spray after doubly.Because this fertilizer only is sprayed onto on the blade face, therefore do not cause any waste, very high by the specific absorption of plant.
Enumerate embodiment and application examples below and further explain the present invention.
Embodiment 1
With 4 tons of ox bone crusher in crushing is particle below the 20mm, and these broken ox bones are packed in the high press-steamed pot, feeds 4.5kg/cm
2The saturation steam of (definitely) pressure keeping vexed jar the steaming 15 minutes under this pressure, stops logical steam, and is to be cooled to 1kg/cm
2From jar, discharge condensation during (definitely) pressure and steam the about 200kg of bone water, carry out so repeatedly 4 times, collect about 800kg altogether and steam bone water, the pH value that will steam bone water with industrial sulphuric acid transfers to 2.5 then, steaming bone water after the acid adjustment is transferred in the autoclave, toward the hoof and the assorted waste materials such as hair, angle that wherein add 300kg pig, ox, then at 1.2kg/cm
2Heating hydrolysis is 5 hours under (definitely) pressure, and is to be cooled to 1kg/cm
2Take the dish out of the pot behind (definitely) pressure, at this moment can be observed the assorted waste material dissolvings fully basically such as hoof, hair, angle of pig, the ox of adding, this shows that protein wherein is transformed into water-soluble protein fully.Remove by filter a spot of insoluble sludge.Obtain 1010kg filtrate this moment altogether approximately, with sodium hydroxide this filtrate is neutralized to PH5, then toward three grades of cultures that wherein add the 250g aspergillus oryzae (5.5 hundred million of interior mycetomes/g), stir, filter, in filtrate, add each 3kg of copper sulfate, zinc sulfate, manganous sulfate, ferrous sulfate and ammonium molybdate, stir, remove by filter small amount of precipitate, the packing liquid end product.
Application examples
In many areas many kinds crop has been carried out repeated test through units such as Northeast Agricultural University, Heilongjiang Institute of Agricultural Sciences, clay fertilizer station, Heilongjiang Province, Harbin City's agricultural technology extension master station, Guangan City, Sichuan Province Institute for New Technologies, test-results shows, Znzyme transformed organic fertilizer of the present invention all has significant volume increase to all kinds of farm crop and improves the effect of quality, and it is as follows that the present will be used for like product (blade face treasured, spray treasured, growth promotor for crop) the institute the results list that the obtains contrast of test statistics result and the prior art of cucumber crop:
The effect of increasing production contrast of the various fertilizer of table 1
Annotate: carry out foliage-spray after various fertilizer dilute 500 times all before use.
From the data of table 1 as can be seen, the effect of increasing production of Znzyme transformed organic fertilizer of the present invention is apparently higher than the foliar applied fertilizer of prior art.What should be noted that especially is that Znzyme transformed organic fertilizer of the present invention does not contain any artificial synthetic organic compound, does not particularly contain harmful ring compound and organic class tethelin etc., therefore human body is not had any disadvantageous effect.
Claims (5)
1, a kind of Znzyme transformed organic fertilizer is characterized in that, it is made by following method:
With the animal bone fragmentation of 100 weight parts, broken bone is put into high press-steamed pot, feed 4-5kg/cm
2The saturation steam of (definitely) pressure keeping vexed jar the steaming more than 15 minutes under this pressure, stops logical steam, is cooled to 1kg/cm
2(definitely) is below the pressure, from jar, discharge condensation and steam bone water, so repeatedly for several times, steaming bone water with the condensation of collecting the 20-30 weight part is as the criterion, the pH value that will steam bone water with acid transfers to 2-3.5, steaming bone water after the acid adjustment is transferred in the autoclave, is selected from one or more mixture in the hoof, angle, hair, feather of animal, keeping 1.05-1.2kg/cm toward wherein adding the 7-15 weight part
2Under (definitely) pressure heating hydrolysis 4-6 hour, cool off, take the dish out of the pot and the elimination throw out, with alkali filtrate is neutralized to PH4-5, include 5 * 10 toward wherein adding the 0.005-0.01 weight part then
8The aspergillus oryzae of individual/above thalline of g (preservation of institute of microbiology of the Chinese Academy of Sciences, preserving number AS.3.951) culture stirs, filter, in filtrate, add each 0.05-0.1 weight part of copper sulfate, zinc sulfate, manganous sulfate, ferrous sulfate and ammonium molybdate, stir the packing finished product.
2, Znzyme transformed organic fertilizer as claimed in claim 1 is characterized in that, wherein said animal bone is ox bone and/or pig bone.
3, Znzyme transformed organic fertilizer as claimed in claim 1 is characterized in that, wherein used acid is sulfuric acid or phosphoric acid when transferring pH value.
4, Znzyme transformed organic fertilizer as claimed in claim 1 is characterized in that, wherein used alkali is sodium hydroxide, yellow soda ash or lime when transferring pH value.
5, as each described Znzyme transformed organic fertilizer of claim 1-4, it is characterized in that, wherein before regulating the pH value that steams bone water, will steam the grease of bone layer waterborne earlier and remove with acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95119430A CN1039639C (en) | 1995-12-29 | 1995-12-29 | Znzyme transformed organic fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95119430A CN1039639C (en) | 1995-12-29 | 1995-12-29 | Znzyme transformed organic fertilizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1133275A true CN1133275A (en) | 1996-10-16 |
| CN1039639C CN1039639C (en) | 1998-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95119430A Expired - Fee Related CN1039639C (en) | 1995-12-29 | 1995-12-29 | Znzyme transformed organic fertilizer |
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| CN (1) | CN1039639C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100371437C (en) * | 2005-02-01 | 2008-02-27 | 湛江海洋大学 | Process for preparing lichem bacillus strain for producing composite amino acid and culture amino acid liquid fertilizer |
| CN102276303A (en) * | 2011-06-16 | 2011-12-14 | 沈保华 | Plant nutrient |
| CN101117300B (en) * | 2007-07-27 | 2012-12-12 | 吴国梁 | Amino acid nitrogen biological organic composite fertilizer and production process thereof |
| CN104003766A (en) * | 2014-06-23 | 2014-08-27 | 蒋常德 | Method for producing amino acid fertilizer by means of waste water and meat and bone meal generated by livestock harmless treatment |
| CN105175158A (en) * | 2015-10-19 | 2015-12-23 | 丁玉琴 | Preparation method for amino-acid agricultural compounded fertilizer |
| CN106187478A (en) * | 2016-07-13 | 2016-12-07 | 石欣 | A kind of fertilizer synergist and the concentrated fertilizer containing this fertilizer synergist |
| CN111170767A (en) * | 2020-03-10 | 2020-05-19 | 滨州市京阳生物肥业有限公司 | Production method of ox bone amino acid organic fertilizer |
-
1995
- 1995-12-29 CN CN95119430A patent/CN1039639C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100371437C (en) * | 2005-02-01 | 2008-02-27 | 湛江海洋大学 | Process for preparing lichem bacillus strain for producing composite amino acid and culture amino acid liquid fertilizer |
| CN101117300B (en) * | 2007-07-27 | 2012-12-12 | 吴国梁 | Amino acid nitrogen biological organic composite fertilizer and production process thereof |
| CN102276303A (en) * | 2011-06-16 | 2011-12-14 | 沈保华 | Plant nutrient |
| CN104003766A (en) * | 2014-06-23 | 2014-08-27 | 蒋常德 | Method for producing amino acid fertilizer by means of waste water and meat and bone meal generated by livestock harmless treatment |
| CN104003766B (en) * | 2014-06-23 | 2016-01-27 | 蒋常德 | The waste water of livestock and poultry harmless treatment and meat meal tankage is utilized to produce the method for amino acid fertilizer |
| CN105175158A (en) * | 2015-10-19 | 2015-12-23 | 丁玉琴 | Preparation method for amino-acid agricultural compounded fertilizer |
| CN106187478A (en) * | 2016-07-13 | 2016-12-07 | 石欣 | A kind of fertilizer synergist and the concentrated fertilizer containing this fertilizer synergist |
| CN111170767A (en) * | 2020-03-10 | 2020-05-19 | 滨州市京阳生物肥业有限公司 | Production method of ox bone amino acid organic fertilizer |
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| Publication number | Publication date |
|---|---|
| CN1039639C (en) | 1998-09-02 |
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