CN113310980A - Method for improving reading accuracy of biochemical analyzer - Google Patents
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- CN113310980A CN113310980A CN202110745773.2A CN202110745773A CN113310980A CN 113310980 A CN113310980 A CN 113310980A CN 202110745773 A CN202110745773 A CN 202110745773A CN 113310980 A CN113310980 A CN 113310980A
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- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0162—Arrangements or apparatus for facilitating the optical investigation using microprocessors for control of a sequence of operations, e.g. test, powering, switching, processing
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Abstract
The invention relates to the technical field of biochemical analyzers, in particular to a method for improving reading accuracy of a biochemical analyzer, which comprises the following steps: s1, initializing the position of the reaction disc; s2, the upper computer sends the pulse number N of the motor needing to move and records the complete pulse number of at least one external tooth of the reaction disc and the potential change of the external tooth optical coupler; s3: analyzing the pulse number Nx and the pulse number Ny corresponding to any one of the two adjacent outer teeth according to the data recorded in S2 to obtain the deviation pulse number N1 of the two adjacent outer teeth; s4, the upper computer orders the reaction disk to return to the initial position again and finishes the offset pulse number N1Then the reaction disc continues to rotate, and the number A of pulses corresponding to the stable value of each cuvette is analyzed1,A2，···AmRecording the number of pulses when the corresponding external tooth optical coupler output signal is changed from high to low as M1，M2,···Mm(ii) a S5 comparing each cuvetteThe corresponding set pulse number Am‑MmThe readings are taken to improve reading accuracy.
Description
Technical Field
The invention relates to the technical field of biochemical analyzers, in particular to a method for improving reading accuracy of a biochemical analyzer.
Background
The biochemical analyzer is one of the important instruments commonly used for clinical diagnosis, and is used for measuring various biochemical indexes such as hemoglobin, cholesterol, myoliver, transaminase, glucose, inorganic phosphorus and the like by analyzing blood and other body fluids, most of the biochemical analyzers work based on the principle of photoelectric colorimetry, a reaction disc is driven to rotate by a motor, and by means of the matching of external teeth of the reaction disc and a photoelectric coupler, a reading module is used for reading a sample in a cuvette to obtain data and transmitting the data to an upper computer for analysis, and in principle, the reading module needs to read the central position of the cuvette, and the reading value at the central position of the cuvette is the most stable value, so that the reading accuracy can be ensured, but the biochemical analyzer in the prior art has inevitable errors due to the external tooth processing of the reaction disc and the installation of the external tooth photoelectric coupler, the result in practice can not make reading module stop in the positive center of cell and carry out the reading for every cell of reading, and the accuracy of reading data direct relation to the accuracy of whole equipment, so all need debug the reading point of every cell in the initial point, the termination point and the motion process when equipment was moved before every equipment was put into use, the debugging needs a large amount of time of wasting, and work load is huge, simultaneously because the opto-coupler triggers by mistake and the reading accuracy difference that the error of reading position caused also remains to be improved.
Disclosure of Invention
The invention provides a method for improving reading accuracy of a biochemical analyzer aiming at the defects in the prior art, and aims to solve the technical problem of poor reading accuracy of the biochemical analyzer in the prior art caused by processing errors and easy false triggering.
The technical scheme for solving the technical problems is as follows: a method for improving reading accuracy of a biochemical analyzer comprises the following steps: s1, the upper computer sends an instruction to initialize the position of the reaction disc;
s2, the upper computer sends the pulse number N of the motor needing to move, the motor receives the command to move the pulse number N, the complete pulse number of at least one external tooth of the reaction disc and the potential change of the optical coupler are recorded in the moving process, and the recorded data are stored;
s3: analyzing the number of pulses Nx when the potential of the external tooth optocoupler changes from high to low and the number of pulses Ny when the potential of the external tooth optocoupler changes from low to high corresponding to any external tooth according to the number of complete pulses of the external teeth and the potential change of the external tooth optocoupler described in S2 to obtain the number of deviation pulses N1 of the external teeth, and storing the number of deviation pulses, wherein N is>400 (N), Ny>Nx,N1＝(Nx+Ny)/2；
S4, the upper computer orders the reaction disk to return to the initial position again and finishes the offset pulse number N1The upper computer controls the reading module to read the point positions of each cuvette, so that the reading values of a plurality of point positions of each cuvette and the corresponding external tooth optical coupler height change values are obtained, and the pulse number value A corresponding to the stable value of each cuvette is analyzed1,A2，···AmRecording the number of pulses of the corresponding external tooth optocoupler output signal from low to high as M1，M2,···MmIf A ism>MmIf not, the step is restarted and the storage is carried out;
s5, the reading module sets the pulse number A according to the corresponding setting of each cuvette in the rotating process of the reaction discm-MmA reading is taken.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, the method for initializing the reaction tray position in step S1 includes: whether the potential change of internal tooth on utilizing the reaction dish and internal tooth opto-coupler judges to be in the initial position, and internal tooth and reaction dish synchronous rotation on the reaction dish when the internal tooth moves to internal tooth opto-coupler department, internal tooth opto-coupler potential can change, and the reaction dish stops this moment, and this position is the initial position of reaction dish promptly, if the internal tooth opto-coupler does not change, then the motor continues the rotation.
Further, the full pulse number of the external teeth in step S2 refers to the corresponding pulse number of the motor from the initialization position to the time when the external teeth enter and completely pass through the external tooth optical coupler.
Further, in step S2, an oscilloscope is used to record the number of complete pulses of the external teeth and the potential change of the external-tooth optical coupler.
Further, the point locations of the cuvette in step S4 correspond to pulses of the motor, that is, each point location of the cuvette corresponds to each pulse value of the motor.
Further, the method for selecting the stable value of the cuvette in step S4 includes: and selecting a plurality of pulse values with the most stable change of the reading value to average.
Further, when the output signal of the external tooth optical coupler in step S4 changes from low to high, the change of the output potential of the external tooth optical coupler is generated at the moment when the external tooth completely passes through the external tooth optical coupler.
Further, in step S4, when each cuvette stops, the pulse difference B corresponding to the change of the output signal of the optical coupler from high to low is recorded at the same time1,B2,···BmWhen the cuvette stops in step S5, recording a pulse difference B when the output signal of the external tooth optical coupler at that time changes from high to low to stopn1Comparison Bn1Whether or not to react with BmIf the comparison result is consistent with the comparison result, the reading operation is ended, otherwise, the reading operation of all the cuvettes is carried out again in the step S5, and when the cuvette stops in the step S5, the pulse difference value B from the high to the stop of the output signal of the corresponding external tooth optical coupler at the moment is recordedn2Comparison Bn2Whether or not to react with BmAnd if so, ending the reading operation, otherwise, resuming the step S4.
The invention has the beneficial effects that: the rotating position of the reaction disc is determined, so that the triggering frequency of the external tooth optical coupler is ensured, accurate reading of the reading module at the central position of the cuvette is realized, the precision requirement on the external tooth processing of the reaction disc and the installation error requirement of the external tooth optical coupler are reduced, the debugging time of a large amount of time for the starting point and the ending point of the reaction disc and the reading point in the movement process before the biochemical analyzer in the prior art is put into use is saved, and the accuracy of reading data is improved; whether the phenomenon of false triggering exists or not is judged by comparing the numerical value of the reading with the pulse numerical value of the outer teeth of the reaction disc corresponding to the stopped cuvette, and the accuracy of the reading data is further improved.
Drawings
FIG. 1 is a schematic top perspective view of a reading device according to the present invention;
FIG. 2 is a perspective view of a reading device according to the present invention from a bottom perspective;
FIG. 3 is a bottom view of the reaction tray and cuvette tray assembled;
FIG. 4 is a perspective view of the reaction tray and cuvette tray assembled;
FIG. 5 is a perspective view of the reaction tray and cuvette tray assembled;
FIG. 6 is a diagram illustrating relative relationship between pulse values according to an embodiment;
FIG. 7 is a flow chart of the method of the present invention.
In the figure, 1, a reaction disc, 2, a cuvette disc, 3, an internal tooth optical coupler, 4, a reading module, 5, an external tooth, 6, a cuvette, 7, an internal tooth and 8, an external tooth optical coupler are arranged.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
A method for improving reading accuracy of a biochemical analyzer is disclosed, referring to attached drawings 1-5, the biochemical analyzer has a corresponding structure of a reaction disk 1, a cuvette disk 2 fixedly mounted on the reaction disk 1, a groove-shaped internal tooth optical coupler 3 mounted below the reaction disk 1 and a reading module 4 mounted on the side of the cuvette disk 2, the bottom surface of the reaction disk 1 is provided with an internal tooth 7 and a plurality of external teeth 5 arranged circumferentially, the cuvette disk 2 is provided with a plurality of cuvettes 6 corresponding to the external teeth 5 of the reaction disk 1 in number, the internal tooth 7 and the external tooth 5 of the reaction disk 1 can enable the internal tooth optical coupler 3 to generate potential change, the positions of the cuvettes 6 correspond to the positions of the external teeth 5 of the reaction disk 1, specifically, connecting lines between the center of each cuvette 6 and the center of the reaction disk 1 are both located between the two external teeth 5 of the reaction disk 1, a motor for driving the reaction disk 1 to rotate for 400 pulses, the reaction disc 1 rotates from the center of one outer tooth 5 to the center of its adjacent outer tooth 5.
The method for reading the biochemical analyzer comprises the following steps: referring to fig. 6 and 7, in step S1, the upper computer sends an instruction, the position of the reaction disk 1 is initialized, specifically, whether the reaction disk is in the initialized position is judged by using the potential change of the internal teeth 7 on the reaction disk 1 and the internal teeth optical coupler 3, the internal teeth 7 on the reaction disk 1 and the reaction disk 1 rotate synchronously, when the internal teeth 7 move to the internal teeth optical coupler 3, the potential of the internal teeth optical coupler 3 changes, at this time, the reaction disk 1 stops, the position is the initialized position of the reaction disk 1, and if the internal teeth optical coupler 3 does not change, the motor continues to rotate;
s2, the upper computer sends a pulse number N of the motor needing to move, wherein N is set to be 700, the motor receives a command to move with the pulse number N being 700, in the clockwise rotation process of the reaction disc 1, the reaction disc 1 is provided with a complete external tooth 5 which passes through the external tooth optical coupler 8, an oscilloscope is adopted to record the complete pulse number of the external tooth 5 of the reaction disc 1 and the potential change of the external tooth optical coupler 8, and recorded data are stored, wherein the complete pulse number of the external tooth 5 refers to the pulse number corresponding to the motor when the external tooth 5 enters and completely passes through the external tooth optical coupler 8 from an initialization position;
s3: analyzing the number Nx of pulses when the potential of the external tooth optocoupler 8 corresponding to any one external tooth 5 changes from high to low and the number Ny of pulses when the potential of the external tooth optocoupler 8 corresponding to the external tooth 5 changes from low to high according to the number of full pulses of the external teeth 5 and the potential change of the external tooth optocoupler 8 described in S2 to obtain the number N1 of deviation pulses of the external teeth 5, and storing the number N of deviation pulsesy>Nx,N1＝(Nx+Ny)/2；
S4 referring to FIG. 6, the host computer commands the reaction tray 1 to return to the initialization position again and completes the number N of offset pulses1The external tooth optical coupler 8 is located at the center of the external teeth 5, the reaction disc 1 continues to rotate, the upper computer controls the reading module 4 to read the point positions of each cuvette 6, the reading values of a plurality of point positions of each cuvette 6 and the corresponding external tooth optical coupler 8 height change values are obtained, and the pulse number value A corresponding to the stable value of each cuvette 6 is analyzed1,A2，···AmRecording the number of pulses when the corresponding output signal of the external tooth optical coupler 8 is changed from low to high as M1，M2,···MmIf A ism>MmIf the result is positive, continuing, otherwise, restarting the step, and simultaneously recording the pulse difference value B when the output signal of each cuvette 6 stops and the corresponding external tooth optical coupler 8 changes from high to low to stop1,B2,···BmAnd storing the data, wherein the point location of the cuvette 6 corresponds to the pulse of the motor, that is, each point location of the cuvette 6 corresponds to each pulse value of the motor, and the method for selecting the stable value of the cuvette 6 comprises the following steps: selecting 16 pulse values with the most stable reading value change to obtain an average value, wherein when the output signal of the external tooth optical coupler 8 is changed from low to high, the output signal of the external tooth optical coupler 8 refers to the change of the output potential of the instant optical coupler of which the external tooth 5 completely passes through the external tooth optical coupler 8, and when the output signal of the external tooth optical coupler 8 is changed from high to low, the output signal of the instant optical coupler of which the external tooth 5 enters the external tooth optical coupler 8 is changed;
s5, reading module 4 sets the pulse number A according to the corresponding setting of each cuvette 6 in the rotating process of reaction disc 1m-MmTaking readings, i.e. when the motor is rotating from the initial position AmAfter a pulse, the external tooth optical coupler 8 is triggered, and when the rotation is continued (A)m-Mm) After each pulse, the reading module 4 reads, the obtained reading is an accurate reading value, after the reading is finished, the cuvette stops, and the pulse difference B from the high level to the low level of the output signal of the corresponding external tooth optical coupler 8 before the recording stops is recordedn1Comparison Bn1Whether or not to react with BmAnd if they are consistent, the reading operation is ended,otherwise, the step S5 is executed again to read all the cuvettes 6, and when the cuvette 6 is stopped in the step S5, the pulse difference B from the high to the low of the output signal of the corresponding external tooth optical coupler 8 before the stop is recordedn2Comparison Bn2Whether or not to react with BmAnd if so, ending the reading operation, otherwise, resuming the step S4.
According to the invention, the rotating position of the reaction disc 1 is determined, and the triggering frequency of the external tooth optical coupler 8 is ensured, so that the reading module 4 can accurately read the central position of the cuvette 6, the precision requirement on the processing of the external teeth 5 of the reaction disc 1 and the installation error requirement of the external tooth optical coupler 8 are reduced, the debugging time of a biochemical analyzer in the prior art for consuming a large amount of time for the starting point, the ending point and the reading point in the movement process of the reaction disc 1 before being put into use is saved, and the accuracy of reading data is improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A method for improving reading accuracy of a biochemical analyzer is characterized in that: the method comprises the following steps: s1, the upper computer sends an instruction to initialize the position of the reaction disc;
s2, the upper computer sends the pulse number N of the motor needing to move, the motor receives the command to move the pulse number N, in the moving process, the complete pulse number of at least one external tooth of the reaction disc and the potential change of the external tooth optical coupler are recorded, and the recorded data are stored;
s3: analyzing the number of pulses Nx when the potential of the external tooth optocoupler changes from high to low and the number of pulses Ny when the potential of the external tooth optocoupler changes from low to high corresponding to any external tooth according to the number of complete pulses of the external teeth and the potential change of the external tooth optocoupler described in S2 to obtain the number of deviation pulses N1 of the external teeth, and storing the number of deviation pulses, wherein N is>400 (N), Ny>Nx,N1＝(Nx+Ny)/2；
S4, the upper computer orders the reaction disk to return to the initial position again and finishes the offset pulse number N1The upper computer controls the reading module to read the point positions of each cuvette, so that the reading values of a plurality of point positions of each cuvette and the corresponding external tooth optical coupler height change values are obtained, and the pulse number value A corresponding to the stable value of each cuvette is analyzed1,A2，···AmRecording the number of pulses of the corresponding external tooth optocoupler output signal from low to high as M1，M2,···MmIf A ism>MmIf not, the step is restarted and the storage is carried out;
s5, the reading module sets the pulse number A according to the corresponding setting of each cuvette in the rotating process of the reaction discm-MmA reading is taken.
2. A method of improving the accuracy of biochemical analyzer readings according to claim 1, wherein: the method for initializing the position of the reaction tray in step S1 includes: whether the potential change of internal tooth on utilizing the reaction dish and internal tooth opto-coupler judges to be in the initial position, and internal tooth and reaction dish synchronous rotation on the reaction dish when the internal tooth moves to internal tooth opto-coupler department, internal tooth opto-coupler potential can change, and the reaction dish stops this moment, and this position is the initial position of reaction dish promptly, if the internal tooth opto-coupler does not change, then the motor continues the rotation.
3. A method of improving the accuracy of biochemical analyzer readings according to claim 2, wherein: the full pulse number of the external teeth in the step S2 refers to the corresponding pulse number of the motor from the initialization position to the time when the external teeth enter and completely pass through the external tooth optical coupler.
4. A method of improving the accuracy of biochemical analyzer readings according to claim 3, wherein: and in the step S2, recording the complete pulse number of the external teeth and the potential change of the external tooth optical coupler by using an oscilloscope.
5. The method of claim 4, wherein the biochemical analyzer comprises: the point locations of the cuvette in step S4 correspond to pulses of the motor, that is, each point location of the cuvette corresponds to each pulse value of the motor.
6. The method of claim 5, wherein the biochemical analyzer comprises: the method for selecting the stable value of the cuvette in the step S4 comprises the following steps: and selecting a plurality of pulse values with the most stable change of the reading value to average.
7. The method of claim 6, wherein the biochemical analyzer comprises: when the output signal of the external tooth optical coupler in the step S4 changes from low to high, the change of the output potential of the external tooth optical coupler is indicated at the moment when the external tooth completely passes through the external tooth optical coupler.
8. The method of claim 7, wherein the biochemical analyzer comprises: in step S4, when each cuvette stops, the pulse difference B between the output signal of the external tooth optical coupler corresponding to each cuvette before the cuvette stops changing from high to low is recorded1,B2,···BmWhen the cuvette stops in step S5, recording a pulse difference B when the output signal of the external tooth optical coupler at that time changes from high to low to stopn1Comparison Bn1Whether or not to react with BmIf the comparison result is consistent with the comparison result, the reading operation is ended, otherwise, the reading operation of all the cuvettes is carried out again in the step S5, and when the cuvette is stopped in the step S5, the pulse difference value B from the high to the low of the corresponding external tooth optocoupler output signal before the stop to the stop is recordedn2Comparison Bn2Whether or not to react with BmAnd if so, ending the reading operation, otherwise, resuming the step S4.
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