CN216594763U - A sample dish that is used for biochemical analyzer of multiple project detection - Google Patents

Abstract

The technical scheme of the invention discloses a sample disc of a biochemical analyzer for detecting various items, which comprises an outer disc, an inner disc, a middle disc and a driving device, wherein the outer disc is provided with a plurality of grooves; the inner disc is of a circular structure, the outer disc and the middle disc are of annular structures, the middle disc is fixed between the inner disc and the outer disc, the inner disc and the middle disc are concentrically arranged, and the output end of the driving device is connected with the inner disc; 40 reagent cups are uniformly distributed on the inner disc and the middle disc respectively, the reagent cups of the middle disc and the reagent cups of the inner disc are aligned along the radial direction, and 48 sample cups are uniformly distributed on the outer disc; the reagent cups of the inner disc, the reagent cups of the middle disc and the sample cups of the outer disc are divided into 8 groups, the first sample cup of each group, the first reagent cup of the middle disc and the first reagent cup of the inner disc form a positioning origin, and the positioning origin of each group is coincided with a sampling motion curve through the rotation of the sample disc; 8 groups of different detection items can be carried out at one time, and the absorbance detection is carried out in time after the reaction of each group of samples is finished, so that the detection result is accurate.

Description

Sample disc of biochemical analyzer for detecting various items

Technical Field

The invention belongs to the technical field of biochemical analysis automatic detection equipment, and particularly relates to a sample disc of a biochemical analyzer for detecting various items.

Background

The technology of the full-automatic biochemical analyzer on the market at present is mature, detection is carried out through reaction of a sample and a reagent, and detection methods are generally divided into three types: end-point methods, rate methods, and two-point methods. The end-point method is that after a period of reaction, the reaction is complete, and all substrates are converted into products; the degree of change in absorbance is proportional to the concentration of the analyte. The velocity method is to find the linearity of a linear detection area, and utilize the least square method to fit a straight line and calculate the velocity. The two-point method is to subtract the absorbance at the sampling starting point from the absorbance at the sampling ending point to obtain the absorbance difference, and the absorbance difference is directly divided by the time for calculation without linear degree detection. The above three methods can be completed in about ten minutes, so that the biochemical analyzer on the market is basically designed to mechanically set a reaction time of 10 minutes for each reagent, which is determined according to the maximum incubation time of 10 minutes for most reagents. For example, a 200-speed biochemical instrument is on the market, wherein the 200-speed instrument realizes the test of 200 samples per hour, and the data is obtained, the reaction time of each batch of samples is set to be 10 minutes, and the detection of a plurality of samples is carried out in each batch. Although each reagent can be ensured to react fully, many reagents do not need 10 minutes of reaction time, for example, an albumin reagent only needs 2 minutes to complete the reaction, and the reagents are divided into single reagents and double reagents, and some items need to be added with a first reagent before detection to eliminate interference, so that the time change is larger.

Patent CN 1093639C discloses an automatic analysis method and apparatus using a plurality of reagents, in which a reaction disk having a plurality of reaction vessels arranged therein is repeatedly rotated 1 cycle by one pitch and stopped to receive a sample, so that reactions with different reaction times can coexist in the same row of reaction vessels. However, when the reaction disk is rotated, the absorbances of all the reaction containers in the light beam passing through the photometer are measured, that is, the absorbances of all the reaction containers are measured every cycle (1 cycle is 18 seconds), and the photometer consumes much energy, and is required to be frequently replaced, which is expensive.

In addition, patent CN 201654030U discloses a reagent sample tray for a full-automatic biochemical analyzer, wherein a sample fixing plate is provided with a round hole for placing a sample cup, a spacer is fixed on the reagent fixing plate, and at least 3 reagent bottles can be placed in the radial direction of the spacer; however, the sample cups and the reagent bottles are aligned in the radial direction, the number of the sample cups is small, and the number of the reagent bottles is large, so that the positions of a plurality of reagent bottles are free, and the space arrangement is not reasonable. Patent CN 205844162U discloses a biochemical analyzer and a sample system thereof, wherein the connecting lines of the sample positions arranged on the inner disc are distributed in two concentric circles along the radial direction of the inner disc, and the connecting lines of the sample positions arranged on the outer disc are distributed in three concentric circles along the radial direction of the outer disc, but the sample positions of the inner disc and the outer disc are not aligned in the radial direction. And the sample disc and the sample system of the 2 patents do not consider the position relation between the sample and the reagent, during detection, the sample disc rotates, the reagent is added after the sample is added, the reaction time set by the biochemical analyzer is the same, and only one detection item can be carried out at one time.

Disclosure of Invention

In order to solve the technical problem, the invention provides a sample disc of a biochemical analyzer for detecting various items, wherein reagent cups of an inner disc, reagent cups of a middle disc and sample cups of an outer disc are equally divided into 8 groups, each group is provided with a positioning origin, a sampling needle can conveniently sample from the positioning origin of each group, different types of samples are detected on each group, and absorbance detection is timely carried out after the reaction of each group of samples is finished.

The technical scheme of the invention is as follows: a sample tray of a biochemical analyzer for detecting a plurality of items comprises an outer tray, an inner tray, a middle tray and a driving device; the inner disc is of a circular structure, the outer disc and the middle disc are of annular structures, the middle disc is fixed between the inner disc and the outer disc, the inner disc and the middle disc are concentrically arranged, and the output end of the driving device is connected with the inner disc; 40 reagent cups are uniformly distributed on the inner disc and the middle disc respectively, the reagent cups of the middle disc and the reagent cups of the inner disc are aligned along the radial direction, and 48 sample cups are uniformly distributed on the outer disc; the reagent cups of the inner disc, the reagent cups of the middle disc and the sample cups of the outer disc are equally divided into 8 groups, the first sample cup of each group, the first reagent cup of the middle disc and the first reagent cup of the inner disc form a positioning origin, and the positioning origin and the sampling motion curve of each group coincide through the rotation of the sample disc.

Furthermore, the reagent cup in the positioning origin is arranged in the clockwise direction of the sample cup, the reagent cup and the sample cup of the middle disc are adjacent, and the included angle between the connecting line of the reagent cup of the inner disc and the center of the concentric circle and the connecting line of the reagent cup of the middle disc and the center of the concentric circle is 18 degrees.

Furthermore, the outer disc is provided with 40 clamping grooves, the middle disc is clamped on the clamping grooves, and the clamping grooves correspond to the reagent cups.

Further, the inner disc and the middle disc are clamped and fixed.

Further, 40 sample cups are uniformly distributed on the middle disc.

A detection method of a biochemical analyzer for detecting various items comprises the following specific steps:

s1, adding samples into the sample cups, adding detection reagents into the reagent cups, dividing the reagent cups of the inner tray, the reagent cups of the middle tray and the sample cups of the outer tray into 8 groups, wherein the items for detection in the sample cups and the reagent cups in different groups are different, and at most 6 samples and 10 reagents are placed in each group; when the sample disc is at the initial position, sampling is carried out from the positioning origin of the first group;

s2, driving a sampling needle to rotate by a mechanical arm of the full-automatic biochemical analyzer, enabling the sampling needle to move along a sampling motion curve, and sampling by the sampling needle along the sample cups and the reagent cups at the first group of positioning original points; after the sampling needle samples, the mechanical arm rotates to drive the sampling needle to move to the upper part of the reaction tank, and the sample and the detection reagent are placed into the reaction tank for reaction; rotating the outer disk, sampling the samples in the other sample cups of the first group, rotating the inner disk and the middle disk, and sampling the detection reagents in the other reagent cups of the first group; after the sampling of one group of sample cups and reagent cups is finished, sampling of the next group of sample cups and reagent cups is carried out;

s3, after the reaction of the sample and the detection reagent in the reaction tank is completed, moving the reaction product in the reaction tank to a cleaning position, cleaning the reaction product, and detecting the absorbance; after the reaction products of one group of samples are cleaned and subjected to absorbance detection, cleaning and absorbance detection are carried out on the reaction products of the other group of samples; and sequentially adding the sample and the detection reagent with gradually increased reaction time into the sample cups and the reagent cups of the first group to the eighth group.

Further, in S3, when the reaction between the current group of samples and the detection reagent is not completed, and when the reaction between the later group of samples and the detection reagent is completed, the reaction product of the later group of samples is first cleaned and absorbance-detected, and after the reaction between the current group of samples and the detection reagent is completed, the reaction product of the former group of samples is moved to a cleaning position, cleaned, and then absorbance-detected.

Further, in S2, the current group of samples needs to react with two detection reagents, the samples and the first detection reagent are sampled, mixed in a reaction cell for incubation, during the incubation period, the next group of samples are sampled until the incubation time of the previous group of samples is over, the sample tray is rotated until the sampling motion curves of the reagent cups of the previous group and the sampling needles coincide, the sampling needles sample the second detection reagent in the reagent cups, and the samples are placed in the reaction cell to react with the incubated samples and the first reagent.

By adopting the technical scheme, the invention has the following beneficial effects:

(1) according to the invention, the reagent cups of the inner disc, the reagent cups of the middle disc and the sample cups of the outer disc are uniformly divided into 8 groups, the first sample cup of each group, the first reagent cup of the middle disc and the first reagent cup of the inner disc form a positioning origin, so that a sampling needle can sample from the positioning origin of each group, and different types of samples of each group are detected, so that the full-automatic biochemical analyzer can perform 8 groups of different detection items at one time, the detection time of each group of detection items is different, and the absorbance detection is performed in time after the reaction of each group of samples is completed, namely when the absorbance detection is performed on a reaction product of a current group of samples, the samples of the latter group are still in the reaction process with the detection reagents, the detection result is accurate, the structure is simple, and the operation is convenient; and each group sets up 1 location initial point, has simplified the software algorithm through machinery, very big reduction the sample dish when rotatory location confusion and operating personnel's misoperation.

(2) A sample is placed in the sample cup on the outer ring of the sample disc, and a detection reagent is placed in the reagent cup on the inner ring, so that the space is greatly saved; and 48 sample cups on the outer disc, 40 reagent cups on the inner disc and the middle disc respectively increase the number of the sample cups, so that the number of the detected samples is increased, and the number of the samples and the detected reagents is matched properly.

(3) Inner disc, well dish and outer dish fixed connection drive inner disc, well dish and outer dish synchronous revolution through a drive arrangement, and set up the location initial point through every group, can reduce because the inaccurate two detection errors that cause of location to and reduce software program error, improved the rate of accuracy that detects and accelerate the time that detects.

(4) Sampling is started from the positioning origin point from each group of sample cups and reagent cups, so that accurate sampling of each group of samples and detection reagents is guaranteed, detection items of each group of samples and detection reagents are different, reaction time is different, cleaning and absorbance detection can be performed in time after reaction is completed, detection is not required to be performed after 10 minutes, accelerated sample addition is realized, detection time is saved, cell necrosis and reagent aging caused by overlong reaction time are prevented, and detection efficiency and accuracy of detection results are improved; and accurate positioning in the detection process can be realized according to different reaction time of each detection item.

Drawings

FIG. 1 is a schematic structural view of example 1;

FIG. 2 is a schematic diagram of the grouping structure of reagent cups and sample cups;

FIG. 3 is a schematic structural view of the outer plate;

fig. 4 is a schematic structural view of embodiment 2.

In the figure, an outer disc 1, an inner disc 2, a middle disc 3, a reagent cup 4, a sample cup 5, a driving device 6, a positioning origin 7, a sampling needle 8, a mechanical arm 9, a sampling motion curve 10, a reaction tank 11, a cleaning position 12 and a clamping groove 13.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

Example 1

Referring to fig. 1 to 4, a sample tray of a biochemical analyzer for multi-item testing includes an outer tray 1, an inner tray 2, an intermediate tray 3, and a driving device 6; the inner disc 2 is of a circular structure, the outer disc 1 and the middle disc 3 are of annular structures, the middle disc 3 is fixed between the inner disc 2 and the outer disc 1, the inner disc 2 and the middle disc 3 are concentrically arranged, and the output end of the driving device 6 is connected with the inner disc 2; 40 reagent cups 4 are respectively and uniformly distributed on the inner disc 2 and the middle disc 3, the reagent cups 4 of the middle disc 3 and the reagent cups 4 of the inner disc 2 are aligned along the radial direction, and 48 sample cups 5 are uniformly distributed on the outer disc 1; the reagent cups 4 of the inner tray 2, the reagent cups 4 of the middle tray 3 and the sample cups 5 of the outer tray 1 are equally divided into 8 groups, the first sample cup 5 of each group, the first reagent cup 4 of the middle tray 3 and the first reagent cup 4 of the inner tray 2 are positioned on the sampling needle 8 to form a positioning origin 7, and the positioning origin 7 and the sampling motion curve 10 of each group coincide through the rotation of the sample tray.

One end of the sampling needle 8 is fixed on the mechanical arm 9, the other end of the sampling needle makes circular rotation motion to form a sampling motion curve 10, the sampling motion curve 10 is superposed with the motion curve of the mechanical arm 9, and the sampling needle 8 samples in any sample cup 5 and reagent cup 5 on the sampling motion curve 10. The reagent cups 4 of the inner tray 2, the reagent cups 4 of the middle tray 3 and the sample cups 5 of the outer tray 1 are divided into 8 groups, and each group is formed by 45 degrees.

The driving device 6 comprises a motor and a gear assembly, the output end of the motor is connected with the gear assembly, the gear assembly is connected with the inner disc 2, when the motor rotates, the gear assembly is driven to rotate, the gear assembly drives the inner disc 2 to rotate, and finally the middle disc 3, the outer disc 1 and the inner disc 2 rotate synchronously.

Furthermore, the reagent cups 4 in the positioning origin 7 are arranged in the clockwise direction of the sample cups 5, the reagent cups 4 of the middle disc 3 are adjacent to the sample cups 5, and an included angle between a connecting line between the reagent cups 4 of the inner disc 2 and the centers of the concentric circles and a connecting line between the reagent cups 4 of the middle disc 3 and the centers of the concentric circles is 18 degrees.

48 sample cups 5 are arranged on the outer disc 1, 40 reagent cups 4 are respectively arranged on the inner disc 2 and the middle disc 3, the included angle between the connecting line of the centers of the reagent cups 4 of the inner disc 2 and the concentric circles and the connecting line of the centers of the reagent cups 4 of the middle disc 3 and the concentric circles is 18 degrees, the number of the sample cups 5 and the number of the reagent cups 4 limit that the first sample cup 5 and the first reagent cup 4 of each group are positioned on the positioning origin, and along with the rotation of the sample disc, the positioning origin 7 of each group is coincided with the sampling motion curve 10. Reasonable layout, high space utilization degree and reasonable quantity setting of detection samples and detection reagents.

Furthermore, the outer disc 1 is provided with clamping grooves 12, the middle disc 3 is clamped on the clamping grooves 13, the number of the clamping grooves 13 is 40, and the clamping grooves 13 correspond to the reagent cups 4.

Further, the inner disc 2 and the middle disc 3 are clamped and fixed. The inner disc 2, the outer disc 1 and the middle disc 3 are simply and stably installed.

Further, 40 sample cups 5 are uniformly distributed on the central disc 3.

The middle disc 3 is provided with a sample cup 5 or a reagent cup 4 which can be arranged according to the actual test requirements such as the number of samples, the type and the number of detection reagents and the like; when the reagent cups 4 are arranged on the middle disc 3, only 6 samples can be detected by each group of sample cups 5 and reagent cups 4, and the number of detection reagents is large; when the sample cups 5 are arranged on the middle disc 3, 10 samples can be detected by each group of sample cups 5 and reagent cups 4, and the number of detection reagents is small.

In the use of the full-automatic biochemical analyzer, the inner disc 2 is driven to rotate through the driving device 6, the middle disc 3 and the inner disc 2 are connected fixedly, the middle disc 3 is connected with the clamping groove of the outer disc 1 in a clamped mode, therefore, when the inner disc 2 rotates, the middle disc 3 and the outer disc 1 can be driven to rotate synchronously with the inner disc 2, the inner disc 2 can be controlled only by using one driving device 6, the middle disc 3 and the outer disc 1 rotate synchronously, the cost is reduced, and the installation difficulty and the error are reduced. Well dish 3 and 2 joint of interior dish, outer dish 1 and 3 joint of well dish mechanically limit the angular relation of interior dish 2, well dish 3 and outer dish 1, and the position is made mistakes when preventing to rotate. When biochemical analysis detection is started, a photoelectric switch of the biochemical analyzer senses that the sample tray is located at an initial position, so that the sampling motion curve 10 of the sampling needle 8 is exactly coincided with the positioning original points 7 of the reagent cups 4 of the first group of the inner tray 2, the reagent cups 4 of the middle tray 3 and the sample cups 5 of the outer tray 1, and sampling errors are prevented.

A detection method of a biochemical analyzer for detecting various items comprises the following specific steps:

s1, adding a sample into the sample cup 5, adding a detection reagent into the reagent cup 4, dividing the reagent cup 4 of the inner tray 2, the reagent cup 4 of the middle tray 3 and the sample cup 5 of the outer tray 1 into 8 groups, wherein the sample cup 5 and the reagent cup 4 in different groups have different detection projects, and each group is provided with at most 6 samples and 10 reagents; when the sample tray is in the initial position, sampling is carried out from the positioning origin 7 of the first group;

the items which can be detected by the full-automatic biochemical analyzer are divided into 8 groups, and the items comprise protein, hormone, glycometabolism, cardiovascular disease detection, serum non-protein nitrogen-containing compounds, hemoglobin, bile acid, blood ammonia, inorganic ions and serum enzyme, wherein the detection items are different, the used detection reagents are different, and the reaction time of the sample and the detection reagents is different.

In addition, after the reaction of one detection item is completed, the absorbance detection is performed in time, that is, when the detection item with long reaction time continues to react, the detection item with short reaction time starts the absorbance detection, so that after the detection item with the longest reaction time completes the reaction, other detection items complete the absorbance detection, and only the absorbance detection needs to be performed on the detection item, thereby saving the detection time. The existing biochemical analyzer detects the absorbance of all reaction products after all samples are reacted, and thus, the time is long.

S2, the mechanical arm 9 of the full-automatic biochemical analyzer drives the sampling needle 8 to rotate, the sampling needle 8 moves along a sampling motion curve 10, and the sampling needle 8 samples along the sample cup 5 and the reagent cup 4 of the first group of positioning original points 7; after the sampling needle 8 takes a sample, the mechanical arm 9 rotates to drive the sampling needle 8 to move to the upper part of the reaction tank 11, and the sample and the detection reagent are put into the reaction tank 11 for reaction; rotating the outer disc 1, the sampling needle 8 samples the samples in the other sample cups 5 of the first group, rotating the inner disc 2 and the middle disc 3, and the sampling needle 8 samples the detection reagents in the other reagent cups 4 of the first group; after the sampling of one group of sample cups 5 and reagent cups 4 is completed, the next group of sample cups 5 and reagent cups 4 is sampled;

the mechanical arm 9 of the full-automatic biochemical analyzer drives the sampling needle 8 to rotate, the sampling needle 8 moves along a sampling motion curve 10, the sampling needle 8 samples a sample of a positioning origin 7 in the first group, then the sample disc rotates in the clockwise direction, the sampling needle samples the 2 nd sample in the first group at the moment, the sample disc rotates in the clockwise direction again, and the sampling needle 8 sequentially samples the 3 rd sample to the 6 th sample; the sample tray is then rotated counter-clockwise back to the initial position and the sampling needle 8 takes a sample of detection reagent from the reagent cup 4 positioned at the origin, and then by rotation of the sample tray the sampling needle 8 takes a sample of detection reagent from the other reagent cup 4. And after the sampling of the first group is finished, the sample disc rotates clockwise, the positioning origin 7 of the second group is superposed with the sampling motion curve 10, the sampling process of the first group is repeated, the sampling of the second group is finished, the processes are circulated again, and the sampling of the third group to the eighth group is finished.

Sample dish is at the uniform velocity rotatory among the current biochemical analyzer, and sampling needle 8 carries out the application of sample at the uniform velocity, and in this embodiment 1, through the setting of adding order and adding time, realizes the acceleration application of sample to the sample of different groups and detect reagent.

S3, after the reaction of the sample and the detection reagent in the reaction tank 11 is completed, moving the reaction product in the reaction tank 11 to the cleaning position 12, cleaning the reaction product, and detecting the absorbance; after the reaction products of one group of samples are cleaned and subjected to absorbance detection, cleaning and absorbance detection are carried out on the reaction products of the other group of samples; the sample cups 5 and reagent cups 4 of the first to eighth groups are sequentially filled with the sample and the detection reagent with gradually increasing reaction time.

Further, in S3, when the reaction between the current group of samples and the detection reagent is not completed, and when the reaction between the later group of samples and the detection reagent is completed, the reaction product of the later group of samples is first cleaned and subjected to absorbance detection, and when the reaction between the current group of samples and the detection reagent is completed, the reaction product of the previous group of samples is moved to the cleaning position 12, and is cleaned, and then subjected to absorbance detection.

Further, in S2, the current group of samples needs to react with two detection reagents, the samples and the first detection reagent are sampled, mixed in the reaction tank 11 for incubation, during incubation, the next group of samples are sampled until the incubation time of the previous group of samples is over, the sample tray is rotated until the sample motion curve 10 of the reagent cup 4 of the previous group coincides with the sampling motion curve 10 of the sampling needle 8, and the sampling needle 8 samples the second detection reagent in the reagent cup 4 and places the second detection reagent in the reaction tank 11 to react with the incubated samples and the first reagent.

Claims (5)

1. A sample tray for a biochemical analyzer for detecting a plurality of items, characterized in that: comprises an outer disc, an inner disc, a middle disc and a driving device; the inner disc is of a circular structure, the outer disc and the middle disc are of annular structures, the middle disc is fixed between the inner disc and the outer disc, the inner disc and the middle disc are concentrically arranged, and the output end of the driving device is connected with the inner disc; 40 reagent cups are uniformly distributed on the inner disc and the middle disc respectively, the reagent cups of the middle disc and the reagent cups of the inner disc are aligned along the radial direction, and 48 sample cups are uniformly distributed on the outer disc; the reagent cups of the inner disc, the reagent cups of the middle disc and the sample cups of the outer disc are equally divided into 8 groups, the first sample cup of each group, the first reagent cup of the middle disc and the first reagent cup of the inner disc form a positioning origin, and the positioning origin and the sampling motion curve of each group coincide through the rotation of the sample disc.

2. A sample tray for a biochemical analyzer for use in detection of a plurality of items according to claim 1, characterized in that: the reagent cup in the positioning origin is arranged in the clockwise direction of the sample cup, the reagent cup and the sample cup of the middle disc are adjacent, and the included angle between the connecting line of the reagent cup of the inner disc and the center of the concentric circle and the connecting line of the reagent cup of the middle disc and the center of the concentric circle is 18 degrees.

3. A sample tray for a biochemical analyzer for use in detection of a plurality of items according to claim 1, characterized in that: the outer disc is provided with 40 clamping grooves, the middle disc is clamped on the clamping grooves, and the clamping grooves correspond to the reagent cups.

4. A sample tray for a biochemical analyzer for use in detection of a plurality of items according to claim 1, characterized in that: the inner disc is fixedly clamped with the middle disc.

5. The sample tray for biochemical analyzer for multi-item test according to any one of claims 1 to 4, wherein: 40 sample cups are uniformly distributed on the middle disc.

Images