JP2001208760A - Composite biochemical/immunological automatic analyzer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To make blood coagulation and fibrinogenolysis measuring items simultaneously measurable on a rotary reactor for biological/immunological measurement without using any additional special unit. SOLUTION: The composite biochemical/immunological automatic analyzer is enabled to perform other measurement than biochemical and immunological measurement with the rotary reactor 2 at a different reaction time or measurement time. The analyzer is constituted in such a way that detectors 4 of the number corresponding to the reaction time or measurement time which is different from the analysis processing speed of biochemical and immunological measurement are arranged around the reactor 2 mounted with measurement cells 1 of the number corresponding to the analysis processing speed and reaction time as detectors for other measurement and, after the reactor 2 is rotated by one time and the arranging pitch of the detectors 4 is rotated for a prescribed period of time, the reactor 2 is stopped for a fixed period of time and the detectors 4 successively make the other measurement. Therefore, the analyzer can make coagulating time measurement which is shorter than the biochemical and immunological measurement while the analyzer makes the biochemical and immunological measurement.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic biochemical / immuno-analytical system which makes it possible to perform another measurement of reaction time and measurement time different from those of biochemical / immunological measurement in parallel using a rotary reactor. About.

[0002]

2. Description of the Related Art FIG. 4 is a diagram for explaining coagulation time measurement, FIG. 5 is a diagram for explaining an example of calculating a difference between a reaction start point and a reaction end point of measurement data, and FIG. It is a figure for explaining a calculation example.

[0003] Blood clinical test devices are classified into coagulation time measuring devices, biochemical analyzers, immunological analyzers and the like. Among them, the biochemical analyzer and the immunoanalyzer require a long measurement time of 10 minutes or more, but the coagulation time measurement device completes the measurement within 10 seconds to at most 3 minutes. In the measurement of the coagulation time, the time (coagulation time) between the reaction start point and the reaction end point shown in FIG. 4 (C) is calculated.
From the raw data shown in FIG. 4B and FIG.
It is necessary to perform a waveform analysis by a second derivative operation, and thus a temporally high-density data is required. Therefore, in the case of coagulation time measurement, the measurement cell into which the sample and the buffer solution are dispensed is incubated and then fixed and installed in the detector.
After dispensing the coagulation initiation reagent, continuous data can be obtained.

On the other hand, biochemical analyzers and immunological analyzers measure the concentration of a reactant (endpoint method) or measure the reaction rate (rate assay method), as shown in FIG. In order to obtain the answer by calculating the difference between the data at the reaction start point and the data at the reaction end point, or by calculating the slope as shown in FIG. 6, it is possible to perform intermittent measurement with a simple operation and a long time. . Therefore, in the case of biochemical / immunoassay, usually, the measurement cells are evenly arranged on the circumference, and the sample dispenser, reagent dispenser, stirrer, etc. are arranged at certain locations and rotated while rotating. The cell is caused to react, and intermittent measurement is performed when passing through a detector arranged at a fixed position, that is, once per rotation of the rotary reactor.

[0005]

As described above, the coagulation time measuring device and the biochemical / immunological analyzer are completely different from each other in their measurement methods, and thus have not been manufactured as the same device. However, blood coagulation / fibrinolysis (fibrinolysis) measurement items include PT (prothrombin time), APTT
(Activated partial thromboplastin time) and other items for measuring coagulation time, as well as many items for biochemical and immunoassay such as FDP and DD dimer, the diagnosis is made by combining both data. Therefore, it is preferable to acquire data at the same time by the same measuring device.

[0006] Therefore, a certain apparatus is provided with both a rotary reactor and a stationary measuring instrument, and dispenses samples and reagents on the rotary reactor side.
After agitation, incubation, etc., the biochemical / immunological measurement items are measured as they are, and the coagulation time is measured by moving the measuring cell to the stationary measuring device by a robot, and the same measuring device, the same Some have achieved time. However, in such a conventional apparatus, a stationary measuring instrument,
Since a special unit such as a cell mobile robot is added, the size of the apparatus is increased and the price is increased due to the addition, which is not preferable.

[0007]

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a method for measuring blood coagulation and fibrinolysis on a rotary reactor for biochemical and immunoassay without adding a special unit. This allows simultaneous measurement of items.

For this purpose, the present invention relates to a combined biochemical / immune automatic analyzer which makes it possible to perform other measurements of reaction time and measurement time different from those of biochemical / immunological measurement in parallel using a rotary reactor. As a detector for the other measurement around a rotating reactor having a number of measurement cells according to the analysis processing speed and reaction time of biochemical / immunoassay, the analysis processing speed and the different reaction time and After arranging the number of detectors according to the measurement time, rotating the rotating reactor for one rotation and the arrangement pitch of the detectors for a predetermined time, stopping for a certain period of time, the detector stops at the stop position, The number of the measuring cells is larger than the number obtained by multiplying the analysis processing speed of the biochemical / immunoassay by the reaction time, and the different reaction time is added to the analysis processing speed. Multiplied by the measurement time Was the number of detectors, and is characterized in that a number obtained by adding 1 by multiplying the number of pitches of the number of the detector.

[0009]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a diagram showing an embodiment of a combined biochemical / immunoautomatic analyzer according to the present invention, FIG. 2 is a time chart of coagulation measurement data and a reaction operation (protocol), and FIG. And a time chart of a reaction operation (protocol). In the figure, 1 is a measuring cell, 2 is a rotary reactor, 3 is a spectroscopic detector, 4 is an LED
2 shows a detector.

In FIG. 1, a rotary reactor 2 is designed so that it can be used for biochemical / immunological measurement with a reaction time of 15 minutes and a coagulation measurement with a measurement time of 3 minutes. Therefore, the rotating reactor 2 has 91
Measurement cells 1 are held, one spectral detector 3 for biochemical / immunological measurement is arranged, and an LED for coagulation measurement is provided.
Fifteen detectors 4 are arranged for every six pitches. Then, only 7 pitches (LED 1) after the last LED detector 4
5 → LED1), which is one pitch more. The rotation reactor 2 operates so as to rotate one rotation and six pitches for a predetermined time (2 seconds) and then stop for a predetermined time (10 seconds). S (MIX) is a sample dispensing position, and R1 and R
Reference numeral 2 denotes a dispensing position of the first reagent and the second reagent, and R3 denotes a dispensing position when a third reagent is further required.
Re-S is a position where the sample concentration is adjusted as needed.

Next, the operation will be described. First, the rotary reactor 1
Rotates 1 rotation and 6 pitches for a predetermined time (2 seconds),
Thereafter, incubation is performed by stopping for a predetermined time (10 seconds). Paying attention to the measurement cell 2 numbered “1”, the first reagent (such as a buffer solution) is dispensed, the sample is dispensed, and the stirring is performed at a predetermined position of the rotary reactor 1, and the incubation is performed 14 times. LED detector 4 in the second operation
After stopping at the position of (LED1), the second reagent is dispensed and stirred. The coagulation measurement items include the coagulation start reagent (second reagent) dispensed as shown in FIG.
The static measurement is performed by the ED detector 4 for 10 seconds. Furthermore, the rotation reactor 1 rotates 1 rotation and 6 pitches in 2 seconds, and the next LED
Stop before detector 4 (LED2). In this way, the measurement cell 1 containing the sample of the coagulation measurement item is
The rotation of the rotation reactor 1 is stopped for 10 seconds at intervals of 2 seconds, and each LED detector 4 (LEDs 3, 4,...)
, And the coagulation time is measured for 3 minutes while moving the 15 LED detectors 4. On the other hand, in the biochemical / immunological measurement, as shown in FIG. 3, the measurement is performed once every 12 seconds when the light passes through the spectroscopic detector 3, so that necessary data is obtained. Then, since the coagulated sample is usually difficult to wash, the measurement cell 1 is thrown away. Therefore, the measurement cell 1 is replaced by a cell exchanger to prepare for receiving a new sample.

[0012] As described above, a plurality of LEs for coagulation measurement are used in the biochemical / immunoassay rotary reactor as detectors for measuring other reaction times and measurement times different from those for biochemical / immunoassay.
Just by arranging the D detector, the stationary measurement can be performed by the normal operation of the biochemical / immunological measurement. In this case, since a series of data is acquired by a plurality of LED detectors, a difference in detection characteristics deteriorates data accuracy.
It is necessary to adjust and match the characteristics of all LED detectors. In this case, if the detection characteristic has linearity, zero / span adjustment is possible, and it is practical to add a correction operation to raw data by measuring an error blank or cell blank and a high-concentration standard substance. In addition, stationary measurement cannot be performed for 2 seconds when the rotary reactor rotates, but when passing through 15 LED detectors, data is obtained every 0.125 seconds. Can also be interpolated.

Next, a description will be given of the relationship among the number of mounted measurement cells, the analysis processing speed, the reaction time, the coagulation measurement time, and the number of LED detectors of the rotary reactor used in the automatic biochemical / immune analyzer according to the present invention. I do.

First, assuming that the analysis processing speed of biochemical / immunological measurement is V (test / min) and the reaction time is B (min), the number N of measurement cells mounted is as follows: N ≧ BV The time of coagulation measurement is C ( min), the number G of mounted LED detectors is: G = CV, and the pitch P at which the LED detectors are arranged is P ≧ N / G = B / C.

Therefore, when applied to the example of the rotary reactor (FIG. 1), the following is obtained. First, the number of reaction cells that can be held by the rotary reactor is (300 tests / 60 minutes) × 15 minutes = 75 cells from an analysis processing speed of 300 tests / hour and a reaction time of 15 minutes. Looking at this in terms of the test interval (cycle), (60 minutes × 60 seconds) / 300 test = 12 seconds, and the rotary reactor and the dispenser operate every 12 seconds to supply the sample. From the reaction time of 15 minutes, the number of reaction cells that the rotary reactor can hold is (15 minutes × 60 seconds) / 12 seconds = 75. In addition, L for coagulation measurement which can be arranged in a rotary reactor
The number of ED detectors is (300 tests / 60 minutes) × 3 minutes = 15 from the coagulation measurement time of 3 minutes, and the pitch at which the LED detectors are arranged is 75/15 = 5 pitches. However, based on this calculation, simply rotating one rotation and five pitches for a predetermined time, and then operating the rotary reactor so as to stop for a certain period of time, causes the measurement cell and the detector to be displaced every measurement. The positional relationship cannot be shifted. Therefore, in order to shift as described above, it is necessary to set 76, which is one more than the calculated value, as the number of reaction cells. In the case of the above embodiment, the pitch is further increased by one pitch to 6 pitches, and the number of reaction cells is increased to 91 (6 × 15 + 1) by adding one to 90 corresponding to the number of LED detectors. .

It should be noted that the present invention is not limited to the above embodiment, and various modifications are possible. For example, in the above-described embodiment, the analyzer has been described that can simultaneously measure the biochemical / immunological measurement and the coagulation measurement. However, the present invention is not limited to the coagulation measurement items, but the reaction speed is high, and any item requiring continuous measurement is required. If this is the case, simultaneous and continuous measurement can be performed in the same manner. Also, the coagulation measurement was performed for 3 minutes, the biochemistry / immunology measurement was performed for 15 minutes, the processing speed was set to 300 tests / hour, and the measurement was performed using the rotary reactor shown in FIG. 1 under the setting conditions of the two-reagent system. Needless to say, if is changed, it will be changed accordingly. Further, the detector is not limited to the spectral detector or the LED detector, and other detectors can be used as appropriate. Although the number of mounted measurement cells is obtained by adding one to the calculated value, the number of measurement cells may be reduced by one and the last pitch may be reduced by one.

[0017]

As is apparent from the above description, according to the present invention, it is possible to perform another reaction time or another measurement time different from the biochemical / immunoassay in parallel using the rotary reactor. A combined biochemical / immuno-automatic analyzer that is a biochemical / immuno-analytical analytical processing speed and as a detector for other measurements around a rotating reactor having a number of measurement cells according to the reaction time, The number of detectors corresponding to the reaction time and the measurement time different from the analysis processing speed are arranged, and the rotation reactor is rotated once and the arrangement pitch of the detectors is rotated for a predetermined time, and then stopped for a predetermined time, and the stop position is set. Since the other measurement is sequentially performed by the detector in the above, the coagulation time measurement with a shorter measurement time can be simultaneously performed by a plurality of detectors arranged in the rotary reactor while performing the biochemical and immunological measurement. . Also,
The number of measurement cells is larger than the number obtained by multiplying the analysis processing speed of biochemical / immunoassay by the reaction time, and the number obtained by multiplying the analysis processing speed by a different reaction time or measurement time is used as the number of detectors. By multiplying the number by the pitch number and adding 1 to the number, it is possible to perform measurement without waste while shifting the measurement positions one by one.

[Brief description of the drawings]

FIG. 1 is a diagram showing an embodiment of a combined biochemical / immune automatic analyzer according to the present invention.

FIG. 2 is a time chart of coagulation measurement data and a reaction operation (protocol).

FIG. 3 is a time chart of biochemical / immunoassay data and a reaction operation (protocol).

FIG. 4 is a diagram for explaining measurement of a coagulation time.

FIG. 5 is a diagram illustrating a calculation example of a difference between a reaction start point and a reaction end point of measurement data.

FIG. 6 is a diagram illustrating a calculation example of a slope of measurement data.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Measurement cell, 2 ... Rotary reactor, 3 ... Spectroscopic detector, 4 ...
LED detector

Claims (2)

[Claims] 1. A combined biochemical / immuno-automatic analyzer which enables a rotary reactor to perform another reaction time or another measurement time different from the biochemical / immunoassay in parallel. -As a detector for the other measurement around a rotary reactor having the number of measurement cells according to the analysis processing speed and reaction time of the immunoassay, according to the analysis processing speed and the different reaction time or measurement time After arranging the number of detectors and rotating the rotation reactor for one rotation and the arrangement pitch of the detectors for a predetermined time, stop for a predetermined time and sequentially perform another measurement by the detector at the stop position. A combined biochemical / immune automatic analyzer characterized by being configured to perform the same. 2. The number of the measurement cells is larger than the number obtained by multiplying the analysis processing speed of the biochemical / immunoassay by the reaction time, and the number obtained by multiplying the analysis processing speed by the different reaction time or the measurement time is detected. 2. The combined biochemical and immunological automatic analyzer according to claim 1, wherein the number is the number of the detectors, and the number of the detectors is multiplied by the number of pitches and one is added.