CN113305146A - Organic contaminated soil bioremediation device and application thereof - Google Patents
Organic contaminated soil bioremediation device and application thereof Download PDFInfo
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- CN113305146A CN113305146A CN202110689995.7A CN202110689995A CN113305146A CN 113305146 A CN113305146 A CN 113305146A CN 202110689995 A CN202110689995 A CN 202110689995A CN 113305146 A CN113305146 A CN 113305146A
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Abstract
The invention belongs to the technical field of organic contaminated soil remediation, and particularly relates to an organic contaminated soil bioremediation device and application thereof. The device comprises a soil biodegradation chamber, a gas collection chamber, an electrolysis chamber and an electric control chamber, wherein the gas collection chamber is communicated with the soil biodegradation chamber and the electrolysis chamber, and the electrolysis chamber is used for generating electrolyzed water reaction and outputting current; the electric control chamber is used for controlling the electrolytic water reaction and current output in the electrolytic chamber; the current and the biological stimulation are jointly used in the soil biodegradation chamber to strengthen the biodegradation process of the organic pollutants. The invention can realize the double strengthening process of the metabolic activity of functional microorganisms in the soil bioremediation room, has the characteristics of skid-mounted design, flexibility and multiple purposes, and has good application value and prospect for polluted soil remediation.
Description
Technical Field
The invention belongs to the technical field of organic contaminated soil remediation, and particularly relates to an organic contaminated soil bioremediation device and application thereof.
Background
Organic contaminated soil is receiving increasing attention as a by-product in the development of the chemical industry. The existence of organic polluted soil limits the land utilization of original sites of enterprises to a certain extent, particularly, under the condition of gradual development of urbanization, chemical enterprises in urban areas move out of the way, and the legacy sites are urgently required to be developed and reused. However, the location of the site and the residential environment around the site determine that large-scale outward transportation and disposal of the contaminated soil is not easy, and has a great secondary pollution hazard. Therefore, the skid-mounted mobile disposal equipment is adopted for in-situ ex-situ disposal of the polluted site, and the skid-mounted mobile disposal equipment is a feasible and convenient disposal method.
The existing skid-mounted soil remediation equipment mainly adopts a soil cleaning process, a chemical oxidation process and a thermal desorption treatment process, and the polluted soil is remediated through a physical or chemical treatment technology. However, the disposal of the cleaning solution in the soil cleaning process is a subsequent troublesome disposal work, especially in the remediation project of organic contaminated sites in urban areas, and the disposal of the cleaning solution becomes a burden that increases the disposal cost of the contaminated soil; on the background of large ecological environment requirements of carbon peak and carbon neutralization, greenhouse gases continuously discharged in the thermal desorption treatment process will limit wider application of the technology in the future, and meanwhile, higher energy consumption load and treatment cost of the greenhouse gases are also one of adverse factors limiting further large-scale application of the technology. In contrast, bioremediation is valued again by environmental workers by virtue of its economical and friendly process characteristics. However, the defects of long period and unstable effect of microbial repair are still negative factors in engineering application. And the in-situ repair period is longer, and the ex-situ repair also relates to the problem of secondary pollution risk of outward transportation. Therefore, the development of an ectopic treatment device with good bioremediation efficiency and stabilization effect is urgently needed, so that a complete set of high-efficiency and low-consumption bioremediation equipment is formed on the basis of a bioremediation technology, and the low-carbon treatment requirement of soil remediation engineering is met.
The existing engineering application mode of the bioremediation technology mainly takes a biological pile remediation process as a main mode, and performs biodegradation reinforcement through methods such as aeration, oxygen supply and the like; however, under an open system, the remediation effect is still uncertain, and the technology can only realize the aerobic degradation process of organic pollutants, but has difficulty in realizing the disposal requirement for the aspect with the anaerobic degradation requirement, such as halogenated hydrocarbon. Therefore, the development requirement of the skid-mounted bioremediation device is met, the biostimulation strengthening and electric strengthening repairing processes are integrated based on the existing microorganism repairing strengthening mode, and the multifunctional device which is efficient and stable and gives consideration to aerobic degradation and anaerobic degradation is developed, so that feasible, reliable and low-carbon bioremediation device equipment is provided for engineering application of the organic contaminated soil repairing industry.
Disclosure of Invention
In view of the above problems, the present invention aims to provide a bioremediation device for organic contaminated soil and applications thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
an organic contaminated soil bioremediation device comprises a soil biodegradation chamber, a gas collection chamber, an electrolysis chamber and an electric control chamber, wherein the gas collection chamber is communicated with the soil biodegradation chamber and the electrolysis chamber, and the electrolysis chamber is used for generating electrolyzed water reaction and current output; the electric control chamber is used for controlling the electrolytic water reaction and current output in the electrolytic chamber; the current and the biological stimulation are jointly used in the soil biodegradation chamber to strengthen the biodegradation process of the organic pollutants.
Two air collecting chambers are respectively arranged on two sides of the soil biodegradation chamber, and the air collecting chambers are communicated with the electrolysis chamber through air guide pipes; the inner wall of the gas collection chamber is provided with a plurality of gas injection holes communicated with the soil biodegradation chamber.
The two gas collection chambers are communicated through a plurality of gas distribution pipes arranged in the soil biodegradation chamber, and a plurality of gas distribution holes are distributed on the side wall of each gas distribution pipe along the axial direction;
and the upper surface of the gas collection chamber is provided with a pressure gauge and a pressure limiting valve.
The electrolytic chamber comprises an anode electrolytic chamber and a cathode electrolytic chamber, electrodes are arranged in the anode electrolytic chamber and the cathode electrolytic chamber, and the electrodes are connected with the electric control chamber through leads;
the anode electrolysis chamber and the cathode electrolysis chamber are respectively communicated with the two gas collection chambers through the gas guide pipe, and the gas guide pipe is provided with a gas valve.
The anode electrolysis chamber and the cathode electrolysis chamber are communicated with the soil biodegradation chamber through a salt bridge, and the salt bridge is used for communicating a conductive circuit;
the anode electrolysis chamber is provided with a liquid injection port A and a liquid discharge port A; the cathode electrolysis chamber is provided with a liquid injection port B and a liquid discharge port B.
The side walls of the two sides of the electrolytic chamber are respectively provided with a box body support, and the bottom of the electrolytic chamber is provided with a foot rest for supporting skid-mounted equipment.
The electric control chamber is arranged between the two gas collecting chambers, a current shunt module and a power supply control system are arranged in the electric control chamber, and the current shunt module is connected with a lead electrolyte connector on the side wall of the electric control chamber through a lead and is connected with electrolyte in the electrolytic chamber to form a current path;
and the power supply control system is connected with the electrodes in the anode electrolysis chamber and the cathode electrolysis chamber through leads.
The outer wall of the electric control chamber is provided with an electrical parameter display screen and a current control valve, the electrical parameter display screen respectively displays power supply voltage, total current intensity in the electrolysis chamber and shunt current intensity flowing through the current shunt module, and the current control valve is used for directly regulating and controlling the shunt current intensity flowing through the current shunt module.
The application of the organic contaminated soil bioremediation device is to produce O by electrolyzing water in an electrolysis chamber2And H2Respectively outputting the organic pollutants to a soil biodegradation chamber, and performing aerobic biodegradation or anaerobic biodegradation of the organic pollutants as an electron acceptor or an electron donor according to biodegradation treatment process conditions to realize a treatment effect of biostimulation reinforcement; meanwhile, the salt bridge is used as a current path between the electrolysis chamber and the soil biodegradation chamber, and direct current flowing through soil in the soil biodegradation chamber plays a role in enhancing the propagation and metabolic activity of microorganisms, so that a current strengthening process is formed.
Controlling the current intensity flowing through the soil in the soil biodegradation chamber to be 50-300 mA;
o supplied from the electrolysis chamber to the soil biodegradation chamber2And H2The content of the (D) can be adjusted by regulating and controlling the current shunt module to adjust the current intensity of a trunk circuit in the device, so that O is realized2And H2The regulation and control method of (1) can be based on the establishment of O2And H2The dosing model of (a), namely:
wherein I is the current intensity of the main circuit, t is the electrifying time, and N iseFor each coulomb corresponding to the number of electrons, η is the effective electron transfer efficiency, i.e. for the production of O2And H2Electron transfer part of (2), NAIs an Avogastron constant; under the condition of determining the current intensity I of the target trunk circuit, the pair is realized
And
the quantitative output of (2).
The invention has the advantages and beneficial effects that:
1) the organic contaminated soil bioremediation device developed by the invention can realize the double strengthening process of the metabolic activity of functional microorganisms in a soil bioremediation room, namely the electric strengthening process under the condition of a direct-current weak electric field and the biostimulation strengthening of the process of generating oxygen and hydrogen by electrolysis, and the double strengthening effect has the coupled correlation characteristic, so that the utilization efficiency and the metabolic capacity of the microorganisms to nutrients and organic contaminated substrates are improved by electric strengthening, and the consumption demand on an electron donor or an electron acceptor is improved; while biostimulation enhancement provides just more H2Or O2As an electron donor or an electron acceptor, thereby meeting the requirements of functional microorganisms on the electron acceptor and the electron donor;
2) the organic contaminated soil bioremediation device developed by the invention realizes the effective current intensity under the condition of a weak electric field through the current shunt module so as to meet the requirement of electric biological enhancement, and simultaneously electrolyzes water through the main current so as to meet the requirement of H2And O2The required amount of (c);
3) the organic contaminated soil bioremediation device developed by the invention is connected with the soil biodegradation chamber and the electrolysis chamber through the salt bridge, so that the extremely acidic or alkaline state at the electrode in the existing electric enhanced biodegradation treatment is avoided, and a good soil pH micro-ecological environment is provided for functional microbial degradation in the soil bio-chamber;
4) the bioremediation device for the organic contaminated soil developed by the invention can utilize the device sealing characteristic thereof in the application process, and combines the supply of an electron acceptor and an electron donor to realize the anaerobic biodegradation and aerobic biological metabolism of organic pollutants in the contaminated soil, and can perform alternate anaerobic and aerobic treatment, thereby achieving the purpose of deep degradation and mineralization of organic pollutants which are difficult to degrade;
5) the organic contaminated soil bioremediation device developed by the invention passes through the established O in the application process2And H2The supply quantity regulation and control model supplies the electron acceptor and the electron donor which meet the degradation requirement of the organic pollution substrate, thereby realizing reasonable O by regulating the current intensity of the main circuit2And H2And (4) preparation.
Drawings
FIG. 1 is a schematic perspective view of an apparatus for bioremediation of organic contaminated soil according to an embodiment of the present invention;
FIG. 2 is a front view of the bioremediation device for organically-polluted soil according to an embodiment of the present invention;
FIG. 3 is a top view of FIG. 2;
FIG. 4 is a schematic front view of a plenum in an embodiment of the invention;
FIG. 5 is a schematic cross-sectional view of an air distribution tube in an embodiment of the present invention;
FIG. 6 is a diagram illustrating the effect of the bioremediation device for organic contaminated soil in remediation of petroleum contaminated soil according to the embodiment of the present invention;
fig. 7 is a diagram illustrating the effect of the bioremediation device for remediating 1,2, 3-trichloropropane contaminated soil according to the embodiment of the present invention.
In the figure: the device comprises a soil biodegradation chamber 1, a gas collection chamber 2, a gas injection hole 3, a gas distribution pipe 4, a pressure gauge 5, a pressure limiting valve 6, a liquid supply port 7, a gas guide pipe 8, a salt bridge 9, a gas valve 10, an electrode 11, a wire 12, an electrolysis chamber 13, an electric control chamber 14, an electrical parameter display screen 15, a current control valve 16, a current shunting module 17, a power control system 18, a salt bridge connector 19, a gas guide port 20, a wire electrolyte connector 21, a box support 22, a skid-mounted equipment foot rest 23, a gas distribution hole 24, a liquid injection port A25, a liquid injection port B26, a liquid discharge port A27 and a liquid discharge port B28.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
As shown in fig. 1-3, the bioremediation device for organic contaminated soil provided by the present invention comprises a soil biodegradation chamber 1, a gas collection chamber 2, an electrolysis chamber 13 and an electric control chamber 14, wherein the gas collection chamber 2 is communicated with the soil biodegradation chamber 1 and the electrolysis chamber 13, and the electrolysis chamber 13 is used for generating electrolyzed water reaction and outputting current; the electric control chamber 14 is used for controlling the electrolyzed water reaction and the current output in the electrolysis chamber 13; the current and the biological stimulation are jointly used for strengthening the biodegradation process of the organic pollutants in the soil biodegradation chamber 1. That is to say, the electric control chamber 14 controls the electrolyzed water reaction and the current output in the electrolytic chamber 13, so that the current and the biological stimulation in the soil biodegradation chamber 1 jointly strengthen the biodegradation process of the organic pollutants, and the ectopic remediation treatment of the organic polluted soil is efficiently completed.
In the embodiment of the invention, a soil biodegradation chamber 1 is positioned above an electrolysis chamber 13, two gas collection chambers 2 are respectively arranged at two sides of the soil biodegradation chamber 1, and the gas collection chambers 2 are communicated with the electrolysis chamber 13 through gas guide pipes 8; the inner wall of the gas collection chamber 2 is provided with a plurality of gas injection holes 3 which are communicated with the soil biodegradation chamber 1, as shown in figure 4.
Further, the two gas collecting chambers 2 are communicated through a plurality of gas distribution pipes 4 arranged in the soil biodegradation chamber 1, and a plurality of gas distribution holes 24 are distributed on the side wall of each gas distribution pipe 4 along the axial direction, as shown in fig. 5;
specifically, two parallel gas guide pipes 8 are arranged on the surface of one side of the gas collection chamber 2, which is back to the soil biodegradation chamber 1, each gas guide pipe 8 is respectively connected with the gas collection chamber 2 through an upper interface, a middle interface and a lower interface, nine gas injection holes 3 are arranged on the surface of one side, which faces the soil biodegradation chamber 1, at equal intervals, the gas collection chambers 2 on the two sides are communicated through four gas distribution pipes 4, gas distribution holes 24 are arranged on each cross section of each gas distribution pipe at equal included angles in the six directions, the distance between the gas distribution holes 24 is 3-5 cm, and the pore diameter of each gas distribution hole 24 is 5-8 mm; the upper surface of the gas collection chamber 2 at one side is provided with a pressure gauge 5 and a pressure limiting valve 6; the outer surfaces of the gas collection chambers 2 at the two sides are respectively provided with a liquid supply port 7 which leads to the soil biodegradation chamber 1, and the liquid supply ports 7 pass through the gas collection chambers 2 and are completely isolated from the gas collection chambers 2; the soil biodegradation chamber 1 can be opened towards two side walls and the upper surface in an extending way and is used for loading and unloading soil materials, the contact edges of the side walls are wrapped by rubber, and the closed state of the soil biodegradation chamber 1 is guaranteed when the device is operated.
As shown in fig. 1-2, in the embodiment of the present invention, the electrolytic chamber 13 includes an anodic electrolytic chamber and a cathodic electrolytic chamber, both the anodic electrolytic chamber and the cathodic electrolytic chamber are provided with electrodes 11, and the electrodes 11 are connected with the electric control chamber 14 through wires 12; the anode electrolysis chamber and the cathode electrolysis chamber are respectively communicated with the two gas collecting chambers 2 through gas guide tubes 8, and gas valves 10 are arranged on the gas guide tubes 8.
Further, the anode electrolysis chamber and the cathode electrolysis chamber are communicated with the soil biodegradation chamber 1 through a salt bridge 9, and the salt bridge 9 is used for communicating a conductive circuit;
furthermore, the anode electrolysis chamber and the cathode electrolysis chamber are both provided with a liquid injection port and a liquid discharge port.
In the embodiment of the invention, the electric control chamber 14 is arranged between the two air collecting chambers 2, the electric control chamber 14 is internally provided with a current shunt module 17 and a power supply control system 18, and the current shunt module 17 is connected with a lead electrolyte connecting port 21 on the side wall of the electric control chamber 14 through a lead and is connected with electrolyte in the electrolytic chamber 13 to form a current path. The current shunting module 17 is used for regulating and controlling the total current and the shunt current in the electrolytic chamber 13 so as to control the current in the soil biodegradation chamber 1; the power control system 18 is connected to the electrodes 11 in the anolyte and catholyte chambers by wires 12 and provides power to the entire apparatus. The power control system 18 can output direct current, and the periodic switching between the positive electrode and the negative electrode is realized by setting the pole inverting period.
Further, an electrical parameter display screen 15 and a current control valve 16 are arranged on the outer wall of the electric control chamber 14, the electrical parameter display screen 15 respectively displays power supply voltage, total current intensity in the electrolytic chamber 13 and shunt current intensity flowing through the current shunt module 17, and the current control valve 16 is used for directly regulating and controlling the shunt current intensity flowing through the current shunt module 17.
Specifically, the electrode 11 is columnar, the electrode 11 is arranged on the bottom surface of the electrolytic chamber 13, and the electrode is replaced through the outer wall of the electrolytic chamber 13 which can be opened or closed; the electrode 11 is connected to a power supply control system 18 through a lead 12 which passes through the electrolytic chamber 13 and is separated from the electric control chamber 14; the upper part of the outer wall of the anode electrolysis chamber is provided with a liquid injection port A25, and the lower part is provided with a liquid discharge port A27; the upper part of the outer wall of the cathode electrolysis chamber is provided with a liquid injection port B26, the lower part is provided with a liquid discharge port B28, and both the liquid injection port and the liquid discharge port can be closed or opened; the outer walls of the two electrolysis chambers 13 are provided with gas guide ports 20, the gas guide ports 20 are connected with gas guide pipes 8 and communicated with the gas collection chamber 2, the gas guide pipes 8 are provided with gas valves 10 near the gas guide ports 20, and the gas flow is regulated and controlled by opening or closing the gas valves 10; meanwhile, the outer walls of the two electrolysis chambers 13 are also provided with salt bridge connecting ports 19, and the salt bridges 9 are respectively communicated with the soil biodegradation chamber 1 and the electrolysis chambers 13 through the salt bridge connecting ports 19 and are used for communicating a conductive circuit. In the embodiment, three groups of salt bridges 9 are respectively arranged on the outer walls of the electrolysis chambers 13 at two sides; the side walls of both sides of the electrolytic chamber 13 are respectively provided with a box body support 22 of the device, and the bottom of the electrolytic chamber 13 is provided with a foot rest 23 for supporting skid-mounted equipment.
The salt bridge 9 forming the current path between the electrolysis chamber 13 and the soil biodegradation chamber 1 can be a saturated potassium sulfate bridge, but is not limited to a saturated potassium sulfate matrix, and is selected according to the fact that the electrolytic discharge activity of the negative ions and the positive ions of the selected matrix is lower than H+And OH-The discharge activity of (3) is preferable; the electrolyte solution used in the electrolytic cell 13 is selected based on the fact that the electrolytic discharge activity of the anions and cations of the selected electrolyte material is lower than that of H+And OH-The discharge activity of (3) is preferable.
The bioremediation device for the organic contaminated soil provided by the invention has double strengthening functions of electric field strengthening and biostimulation strengthening, is suitable for skid-mounted devices for bioremediation of the organic contaminated soil under aerobic and anaerobic conditions, and has good ectopic efficient remediation application potential of the organic contaminated soil.
The organic contaminated soil bioremediation device of the above embodiment is applied by generating O by electrolyzing water in the electrolysis chamber 132And H2Respectively outputting the organic pollutants into the soil biodegradation chamber 1, and performing aerobic biodegradation or anaerobic biodegradation of the organic pollutants according to biodegradation treatment process conditions as an electron acceptor or an electron donor to realize a treatment effect of biostimulation reinforcement; meanwhile, the salt bridge 9 is used as a current path between the electrolysis chamber 13 and the soil biodegradation chamber 1, and direct current flowing through the soil in the soil biodegradation chamber 1 plays a role in enhancing the propagation and metabolic activity of microorganisms, so that a current strengthening process is formed. Further, current reinforcement and biostimulation reinforcement are carried out simultaneously, so that the reproductive metabolic activity of the functional microorganisms is enhanced, the content requirement on an electron acceptor or an electron donor is increased, the requirement is met through biostimulation supply, the degradation capability of the functional microorganisms is enhanced to a greater extent, and the optimal reinforcement effect is realized.
Furthermore, the current intensity flowing through the soil in the soil biodegradation chamber 1 is controlled within the range of 50-300 mA, so that the strengthening effect of the microbial degradation activity is effectively achieved; the electrolysis chamber 13 supplies O into the soil biodegradation chamber 1 as an electron acceptor or electron donor according to the biodegradation treatment process condition2And H2The content of (2) can be adjusted by regulating and controlling the current shunt module 17 to adjust the current intensity of the trunk circuit in the device, so as to realize O2And H2Based on the characteristic requirement of meeting the biological metabolism rate of the organic pollutants.
The current intensity of a trunk circuit in the device is adjusted by regulating and controlling the current shunt module 17, so that O is realized2And H2The regulation and control method of (1) can be based on the establishment of O2And H2The dosing model of (a), namely:
wherein, I is the main circuit current intensity (A), t is the electrifying time(s), NeThe corresponding number of electrons is 6.25 multiplied by 10 for each coulomb18Eta is the effective electron transfer efficiency, i.e. for the production of O2And H2Electron transfer part of (2), NAIs Avogastro constant 6.02 x 1023(ii) a Under the condition of determining the current intensity I of the target trunk circuit, the pair is realized
And
the quantitative output of (2).
The treatment of the organic contaminated site by using the organic contaminated soil bioremediation device provided by the invention is described in detail with reference to the embodiment.
Example one
Ectopic bioremediation treatment for petroleum-contaminated soil in certain oil field area
The petroleum polluted soil is obtained from the ground crude oil polluted soil which is omitted in the peripheral operation process of a well mouth of a certain oil field area, and the polluted soil is treated by adopting the enhanced bioremediation device provided by the invention. Bacillus cereus W1(CN105602869B), Halomonas xianhensis (CN 107828684B) and Bacillus licheniformis (CN 107828684B) which are suitable for electric remediation are selected as petroleum hydrocarbon degradation functional bacteria agents for enhanced biodegradation. Selecting the three thalli stored in a slant culture medium (beef extract peptone culture medium), inoculating the three thalli to the beef extract peptone culture medium, and carrying out shaking culture at the temperature of 30 ℃ and at the speed of 180rpm for 48-72 h until the thalli reach OD600And (5) finishing the culture when the concentration reaches more than 2.0, and keeping the bacterial liquid for later use.
The used inorganic salt liquid culture medium comprises the following components: 1.0g/L NaCl, 0.3g/L MgSO4，1.5g/L NH4NO3，1.5g/L K2HPO4，0.5g/L KH2PO4，0.02g/L CaCl2，0.02g/L FeSO41L of distilled water, pH 7.0-7.5, and sterilizing at 121 ℃ for 20min for later use.
Firstly, impurity removal pretreatment is carried out on petroleum polluted soil, impurity garbage and large gravel are sieved out, then an Allu hopper is used for crushing materials, the polluted soil is crushed into a particle size state below 2mm, the prepared bacterial liquid is mixed into the polluted soil, and the final concentration of the mixed bacterial microorganisms reaches 5.1 x 108CFU/g dry soil, and simultaneously, the water content in the soil reaches the level of 20-25%. And transferring the contaminated soil mixed with the microbial inoculum to a soil biodegradation chamber 1 of the enhanced bioremediation device, closing a top sealing cover, and starting enhanced remediation treatment. The volume of soil in the device in this example reaches 0.48m3(1.0 m.times.0.8 m.times.0.6 m), about 0.72t contaminated soil, the initial oil content of the contaminated soil reached 4.25% (w/w), and for the purpose of comparing the superiority of the treatment effect of the enhanced bioremediation device (EK-BIO-Eh), a general electrokinetic remediation treatment group (EK-BIO) and a control group (CK) without treatment were set, and the parameter settings of each treatment group are shown in Table 1. In the EK-BIO-Eh treatment group, the power supply voltage is set to be 28V through the electrical parameter display screen 15 in the electric control room 14, the inverse polarity period of the voltage polarity is 2h, and based on the soil property and the regulated water content characteristic, the electric enhancement current intensity flowing through the soil biodegradation room and displayed on the electrical parameter display screen 15 through data collected by a current sensor after the device is electrified is 80-120 mA; the salt bridge used in the device is saturated K2SO4Agar salt bridges for the preparation of O by electrolysis of water2Has a total current intensity of 90A, using O2The daily O is estimated by the dosing model of2The supply amount is 20.19mol/d, and the requirement of the amount of the electron acceptor required by daily biodegradation of petroleum hydrocarbon is met; in addition, water is supplied from the liquid supply port 7 once every 20 days according to the actual soil water content requirement, and soil samples are collected once every 10 days to measure the residual amount of petroleum hydrocarbon.
After 100 days of restoration treatment, the residual amount of petroleum hydrocarbon changes, as shown in fig. 6, the degradation rate of petroleum hydrocarbon in EK-BIO treatment group reaches 43.54 +/-1.15%, while the degradation rate of petroleum hydrocarbon in soil restored by the reinforced biological device reaches 56.53 +/-4.3%, and the degradation rate is improved by about 13%. Therefore, on the basis of the electric enhanced microbial remediation (EK-BIO), the supply of the electron acceptor further enhances the microbial metabolism efficiency, and obtains better enhanced bioremediation effect of the petroleum-polluted soil.
TABLE 1
Example two
Ectopic bioremediation treatment for 1,2, 3-trichloroethane polluted soil in certain chemical plant area
Removing impurities from 1,2, 3-trichloroethane polluted soil of a chemical plant area according to the method of the embodiment I, crushing and screening, preparing a microbial inoculum by utilizing Acinetobacter sp.JWDH2-5 according to the method, mixing the microbial inoculum into the polluted soil containing 1,2, 3-trichloroethane with the concentration of 35mg/kg, and enabling the abundance of microorganisms in the soil to reach 6.2 x 108CFU/g dry soil. Loading the same amount of soil as that in the first embodiment into the enhanced biological device, regulating and controlling the total current intensity to 32A through the current splitting module 17, and performing enhanced biological remediation treatment by adopting an anaerobic/aerobic alternative treatment process mode, namely: firstly, carrying out anaerobic stage restoration, when the accumulated anaerobic restoration time reaches 20d, converting into aerobic restoration time with the aerobic restoration period being 20d, then converting into the anaerobic restoration time with the restoration time being 20d, and finally converting into aerobic restoration time with the total restoration time being 80 d. In the operation process of the device, when the device is subjected to anaerobic treatment, the gas valve 10 on the gas guide pipe 8 is connected with the electrolytic chamber 13 with the anode, and the gas valve 10 on the cathode side is opened, so that the soil biodegradation chamber 1 is filled with H2To finishFor the dechlorination of 1,2, 3-trichloropropane, in this stage H2The amount of supply of (A) is about 14.22 mol/d; in the aerobic repair stage, the anode side gas valve 10 is opened, and the cathode side gas valve 10 is closed, so that oxygen is supplied and filled in the soil biodegradation chamber 1, and the process from complete degradation of the intermediate product after dechlorination of 1,2, 3-trichloropropane to mineralization is completed, wherein in the stage, O is generated2The amount of (2) supplied was 7.12 mol/d. In the electric treatment process, because the electrode reversing treatment is carried out in the same way, the electrode reversing period is also 2 hours, and along with the electrode reversing, the anode side and the cathode side gas valve 10 automatically open and close along with the switching of the electrode direction, so as to ensure the anaerobic or aerobic state in the determined soil biodegradation chamber 1. In the 80d treatment process, the pH ranges of the polluted soil in the soil biodegradation chamber 1 at all positions are 6.71-7.13, so that the operation process of the device maintains good soil acid-base conditions for functional microorganisms. In the aerobic mineralization process, the oxygen is accompanied by O2For continuous supply of CO2The pressure limiting valve 6 is promoted to be automatically opened and closed randomly, and the maximum pressure in the soil biodegradation chamber 1 is kept to be not higher than 1.5 atm. In the soil remediation process, sampling a soil sample every 10 days, and determining the residual amounts of 1,2, 3-trichloropropane and dechlorinated chloropropene.
As shown in fig. 7, as the anaerobic and aerobic treatment processes continue alternately, the content of 1,2, 3-trichloropropane shows a significant decrease trend in the two anaerobic stages, and the content of chloropropane shows a significant increase trend in the two corresponding stages; in the aerobic stage, the content of the 1,2, 3-trichloropropane is also reduced, but the speed is slower, and the content of the chloropropane is obviously reduced, which shows the efficient mineralization process of the functional microorganisms in the aerobic stage. The experimental procedure was also set with conventional electrokinetic enhanced microbial treatment (Ge) to compare with the enhanced bioremediation device treatment (Eh) procedure. The result shows that under the condition that the variation trends of the contents of the 1,2, 3-trichloropropane and the chloropropane are similar, the degradation rate of the 1,2, 3-trichloropropane in the Eh treatment group reaches 1.5 times of that of the Ge group, and the residual amount of the chloropropane is only about 1/4 of the Ge group, so that the bioremediation device for the organic contaminated soil, provided by the invention, has a good contaminated soil treatment effect for the organic pollutants such as chlorohydrocarbon and the like which need anaerobic conditions and aerobic conditions for thorough mineralization and removal, and therefore has good market popularization and application prospects.
The invention provides an organic contaminated soil bioremediation device based on electric field enhanced biostimulation, which comprises a soil biodegradation chamber, an electrolysis chamber, an electric control chamber and air guide and conductive pipeline accessories, wherein the electric control chamber outputs current to the interior of the device, and the salt bridge conduction is utilized to realize the process of enhancing the biodegradation of organic pollutants in the soil biodegradation chamber; water electrolysis by regulating main circuit current by current division module to generate O2And H2Realize the indoor O of soil biodegradation through an air duct2And H2Supplying to realize the strengthening constitution of the biostimulation; by using O2And H2The yield estimation model combines the biodegradation characteristics of the polluted substrate to optimize the yield2And H2The supply amount is increased, so that the anaerobic or aerobic high-efficiency biodegradation of organic pollutants in the polluted soil is realized by the device. The device is designed in a skid-mounted manner, has the characteristics of flexibility and multiple purposes, and has good contaminated soil remediation application value and prospect.
The above description is only an embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, improvement, extension, etc. made within the spirit and principle of the present invention are included in the protection scope of the present invention.
Claims (10)
1. The organic contaminated soil bioremediation device is characterized by comprising a soil biodegradation chamber (1), a gas collection chamber (2), an electrolysis chamber (13) and an electric control chamber (14), wherein the gas collection chamber (2) is communicated with the soil biodegradation chamber (1) and the electrolysis chamber (13), and the electrolysis chamber (13) is used for generating electrolyzed water reaction and outputting current; the electric control chamber (14) is used for controlling the electrolyzed water reaction and the current output in the electrolysis chamber (13); the current and the biological stimulation are used together in the soil biodegradation chamber (1) to strengthen the biodegradation process of the organic pollutants.
2. The bioremediation device of organically-polluted soil according to claim 1, wherein two of the collection chambers (2) are respectively provided at both sides of the soil biodegradation chamber (1), and the collection chambers (2) are communicated with the electrolysis chamber (13) through gas ducts (8); the inner wall of the gas collection chamber (2) is provided with a plurality of gas injection holes (3) communicated with the soil biodegradation chamber (1).
3. The bioremediation device for organically-polluted soil according to claim 2, wherein the two air collection chambers (2) are communicated by a plurality of air distribution pipes (4) disposed in the soil biodegradation chamber (1), and a plurality of air distribution holes (24) are axially disposed on a side wall of each air distribution pipe (4);
and a pressure gauge (5) and a pressure limiting valve (6) are arranged on the upper surface of the gas collection chamber (2).
4. The bioremediation device for organically-polluted soil according to claim 2, wherein the electrolysis chamber (13) comprises an anode electrolysis chamber and a cathode electrolysis chamber, each of the anode electrolysis chamber and the cathode electrolysis chamber is provided with an electrode (11), and the electrodes (11) are connected with the electric control chamber (14) through a lead (12);
the anode electrolysis chamber and the cathode electrolysis chamber are respectively communicated with the two gas collection chambers (2) through the gas guide pipes (8), and gas valves (10) are arranged on the gas guide pipes (8).
5. The bioremediation device of organically contaminated soil according to claim 4, wherein said anodic electrolytic chamber and said cathodic electrolytic chamber are in communication with said soil biodegradation chamber (1) through a salt bridge (9), said salt bridge (9) being for conductive circuit communication;
the anode electrolysis chamber is provided with a liquid injection port A (25) and a liquid discharge port A (27); the cathode electrolytic chamber is provided with a liquid injection port B (26) and a liquid discharge port B (28).
6. The bioremediation device for organic contaminated soil according to claim 4, wherein box supports (22) are respectively provided on the side walls of both sides of the electrolysis chamber (13), and a skid-mounted equipment support foot stand (23) is provided at the bottom of the electrolysis chamber (13).
7. The bioremediation device for organically-polluted soil according to claim 4, wherein the electric control chamber (14) is disposed between the two air collection chambers (2), a current distribution module (17) and a power control system (18) are disposed in the electric control chamber (14), and the current distribution module (17) is connected to a lead electrolyte connection port (21) on a side wall of the electric control chamber (14) through a lead and is connected to an electrolyte in the electrolysis chamber (13) to form a current path;
the power supply control system (18) is connected with the electrodes (11) in the anode electrolysis chamber and the cathode electrolysis chamber through leads (12).
8. The organic contaminated soil bioremediation device of claim 7, wherein an electrical parameter display screen (15) and a current control valve (16) are installed on an outer wall of the electric control chamber (14), the electrical parameter display screen (15) displays a power supply voltage, an overall current intensity in the electrolysis chamber (13) and a shunt current intensity flowing through the current shunt module (17), respectively, and the current control valve (16) is used for directly regulating the shunt current intensity flowing through the current shunt module (17).
9. Use of the bioremediation device for organically contaminated soil according to any one of claims 1-8, wherein O generated by electrolyzing water in the electrolysis chamber (13)2And H2Respectively outputting the organic pollutants into the soil biodegradation chamber (1), and performing aerobic biodegradation or anaerobic biodegradation of the organic pollutants according to biodegradation treatment process conditions as an electron acceptor or an electron donor to realize biostimulation enhanced treatment effect; meanwhile, the salt bridge (9) is used as a current path between the electrolysis chamber (13) and the soil biodegradation chamber (1), and direct current flowing through the soil in the soil biodegradation chamber (1) plays a role in enhancing the propagation and metabolic activity of microorganisms, so that a current strengthening process is formed.
10. The application of the bioremediation device for organically-polluted soil according to claim 9, wherein the intensity of the current flowing through the soil in the soil biodegradation chamber (1) is controlled to be 50 to 300 mA;
o supplied from the electrolysis chamber (13) to the soil biodegradation chamber (1)2And H2The content of the (D) can be adjusted by regulating and controlling the current shunt module (17) to adjust the current intensity of a main circuit in the device, so as to realize O2And H2The regulation and control method of (1) can be based on the establishment of O2And H2The dosing model of (a), namely:
wherein I is the current intensity of the main circuit, t is the electrifying time, and N iseFor each coulomb corresponding to the number of electrons, η is the effective electron transfer efficiency, i.e. for the production of O2And H2Electron transfer part of (2), NAIs an Avogastron constant; under the condition of determining the current intensity I of the target trunk circuit, the pair is realized
And
the quantitative output of (2).
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