CN104801537A - Electric-microbial combined remediation method for heavy metal contaminated soil - Google Patents
Electric-microbial combined remediation method for heavy metal contaminated soil Download PDFInfo
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- CN104801537A CN104801537A CN201510163849.5A CN201510163849A CN104801537A CN 104801537 A CN104801537 A CN 104801537A CN 201510163849 A CN201510163849 A CN 201510163849A CN 104801537 A CN104801537 A CN 104801537A
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Abstract
The invention relates to an electric-microbial combined remediation method and an electric-microbial combined remediation device for heavy metal contaminated soil. The electric-microbial combined remediation device comprises a pretreatment system, a direct current stabilized power supply, an electric electrode and an aftertreatment system; a cathode and anode electrolytic tank is arranged on the electric-microbial combined remediation device, the electrode is directly inserted into soil, so that the technology is relatively close to reality, and the direct current stabilized power supply is directly connected with a graphite plate in the electrode; an electric pretreatment system comprises indigenous microorganism cultivation and acclimation. The electric-microbial soil remediation technology can effectively and quickly solve the problems that the conventional soil remediation technology is high in processing cost and can easily cause secondary pollution.
Description
Technical field
The present invention relates to heavy-metal contaminated soil electronic-microorganism coupling treatment technology and device thereof, belong to technical field of soil remediation.
Background technology
In the improvement process of Chinese Urbanization and city habitat environment, because industrial structure upgrading, city layout adjustment and polluting such as to disturb residents at the enterprises remove and be retained in the industrial pollution place of urban area, surrounding enviroment and residents ' health are seriously threatened.According to statistics, 2001-2008, closing down, suspending operations, merging with other plants, changing the products or moving the plants to other places enterprise of China number rapidly increases to 22488 by 6611, and sum reaches more than 100,000.2008, Beijing, Tianjin, Shanghai, these 4 closing down, suspending operations, merging with other plants, changing the products or moving the plants to other places enterprise of municipality directly under the Central Government numbers of Chongqing accounted for 0.68%, 0.50%, 2.78%, 2.66% of industrial enterprise's number then respectively.A large amount of industrial sites ruins, and the industrial ruins constantly occurred with urbanization propelling and industry restructuring in the future, especially the heavy polluted industry of developed regions leaves over industrial sites, be China city must faced by society, economy and ecological environment problem.
Contaminated site recovery technique determines that contaminated site repairs the key link of success or failure.At present, although develop more contaminated soil remediation method, portion of techniques is not suitable for using in industrial pollution place because of quadratic risk function, repairing efficiency and other restrictive conditions.Application has been limited as easily produced bioxin in burning process; The limitation that phytoremediation technology repairs the degree of depth and repairing efficiency because of it is seldom applied to industrial pollution place.Therefore, at present for the reparation of urban industry contaminated site, there is the developing direction that two important: 1, develop recovery technique that is efficient, cheap, green and low energy consumption.As the pollution abatement by the generating process of nature existence itself of microorganism remediation technology, and in repair process the not structure of spoiled soil and ecological functions, do not produce secondary pollution, there is the feature of cheapness " green " low energy consumption.2, apply the integrated solution of multiple recovery technique and administer soil pollution, as associating microorganism/plant and technology of Electrodynamics Renovation.
Microorganism remediation technology is that microorganism heavy metal element has certain adaptation and tolerance, organise in process at heavy metal in soil, edaphon heavy metal has absorption and suction-operated, or change environmental condition by microorganism, thus change metallic element form and reduce the toxicity of metallic element, the transfer ability of enhancing metallic element.Komarova etc. have studied bacillus megaterium (Bacillus megatherium) to the suction-operated of lead, nickel, the cobalt aqueous solution, result shows absorption by microorganism heavy metal or suction-operated, in soil, the nickel of solvable condition is converted into weak obligate ADSORPTION STATE or organically combines state nickel, decreases the toxicity of nickel in soil.Microorganism defines a series of vivo and vitro resistance patience mechanism in these heavy metal environment; comprise isolation mech isolation test in protective mechanism, cell, active efflux system mechanism, enzyme detoxification mechanism and biological methylation, volatilization, redox carry out biology conversion; according to the microbiological mining technology of characteristic development formation, the treatment technology, Techniques of Desulfurization for Burning Coal etc. of heavy metal wastewater thereby of mentioned microorganism; be applied in production practices, and achieve good economy, society and environmental benefit.Therefore, this also makes the improvement utilizing microorganism to carry out heavy-metal contaminated soil become possibility.
Electric driving method has efficiently, fast, be suitable for cohesive soil and can not produce the advantages such as large disturbance to environment, receives extensive concern.Electric repairing technique, as one initiatively recovery technique, can obtain good repairing effect.Electric driving method and permeable reactive wall (PRB) coupling can by PRB(as various active materials such as Zero-valent Iron, active carbon, flyash, zeolites) adsorb fixing by electrokinetic migration to the pollutant at the two poles of the earth, save the pollutant extraction system of dynamic electricity, optimize electrokinetic system repairing performance.
Summary of the invention
The object of this invention is to provide a kind of heavy-metal contaminated soil electronic-microbial association restorative procedure.
1, heavy metal contaminants soil is electronic-a microbial association restorative procedure, it is characterized in that having following process and step:
A the specific microorganism fungus kind of () chosen in advance, chooses contaminated soil, experimental analysis heavy metal in soil content after field sampling, and wherein heavy metal cadmium concentration is the highest, reaches 100mg/kg, and choosing cadmium is target heavy metal; Cultivated by contaminated soil sieve bacterium, find that Acidithiobacillus thiooxidans is dominant bacteria and carries out tolerance domestication; Screening indigenous microorganism also carries out heavy metal tolerance domestication to it, and investigates the activation effect of microorganism heavy metal; After microorganism fungus kind is incubated in fluid nutrient medium, by the microorganism species filtered out cultivate breeding be made into microbial inoculum after save backup;
B () processes in electric device and electrolytic cell; Add above-mentioned special microbial inoculum in experiment pedotheque, pedotheque is placed in the electrolytic cell of perspex transparent cell body, microorganism is carried out to the cultivation of about 15 days, make flora activate heavy metal; Then adopt unidirectional electrode to run, under voltage gradient is 1V/cm condition, carries out energising 10 days; Remove soil in sample region after energising terminates, and then obtain the soil removing heavy metal cadmium.
2, a kind of heavy-metal contaminated soil is electronic-and microbial association restorative procedure device used is common conventional electrolytic cell assembly; Include reactive tank, anode, negative electrode, dc source; Electric device and cell reaction groove adopt pmma material, and electrode material adopts graphite electrode material, is provided with electrode groove, fixed electrode in electrolytic cell; Pending pedotheque is placed with in groove.
Accompanying drawing explanation
Fig. 1 is composite electric of the present invention-microorganism remediation device schematic diagram.
Detailed description of the invention
In the present embodiment, adopt composite electric-microorganism remediation device, see accompanying drawing 1, it comprises dc source, graphite electrode plate and lucite reactive tank; Reactive tank length is 200 mm, width 90 mm, and 1.5 kg pedotheques are laid in cell reaction groove by battery lead plate thickness 4 mm, highly about 50 mm, and in experiment, electrolytic cell pedotheque is artificially contaminated soil, wherein cadmium (Cd
2+) pollutant levels are 100 mg/kg.Dc source is connected with battery lead plate.In the present embodiment, to Shanghai City Golconda south, major region factory samples with leaving over, and find that in contaminated soil sample, cadmium metal content is the highest, reach 100 mg/kg, choosing cadmium is target heavy metal.From soil, screen microorganism, Acidithiobacillus thiooxidans is dominant bacteria, and has activation to target contaminant cadmium (Cd), and be positioned in culture medium after enriching and purifying, its constituent is: 0.2g (NH
4)
2sO
4.0.5g MgSO
4.CaCl
2.KH
2PO
4。feSO
4; Bacterium liquid is added in pedotheque and cultivates 15 days.Microorganism cultivates the voltage gradient energising terminating rear employing 1V/cm, and conduction time is 10 days.Cadmium in Soil (the Cd of rear side
2+) to reduce to 62.5 mg/kg clearances be 37.5% to concentration.Be used alone electric repairing technique in contrast test, clearance is only 19.3%.
Claims (2)
1. heavy metal contaminants soil is electronic-a microbial association restorative procedure, it is characterized in that having following process and step:
A the specific microorganism fungus kind of () chosen in advance, chooses contaminated soil, experimental analysis heavy metal in soil content after field sampling, and wherein heavy metal cadmium concentration is the highest, reaches 100mg/kg, and choosing cadmium is target heavy metal; Cultivated by contaminated soil sieve bacterium, find that Acidithiobacillus thiooxidans is dominant bacteria and carries out tolerance domestication; Screening indigenous microorganism also carries out heavy metal tolerance domestication to it, and investigates the activation effect of microorganism heavy metal; After microorganism fungus kind is incubated in fluid nutrient medium, by the microorganism species filtered out cultivate breeding be made into microbial inoculum after save backup;
B () processes in electric device and electrolytic cell; Add above-mentioned special microbial inoculum in experiment pedotheque, pedotheque is placed in the electrolytic cell of perspex transparent cell body, microorganism is carried out to the cultivation of about 15 days, make flora activate heavy metal; Then adopt unidirectional electrode to run, under voltage gradient is 1V/cm condition, carries out energising 10 days; Remove soil in sample region after energising terminates, and then obtain the soil removing heavy metal cadmium.
2. a heavy-metal contaminated soil is electronic-and microbial association restorative procedure device used is common conventional electrolytic cell assembly; Include reactive tank, anode, negative electrode, dc source; Electric device and cell reaction groove adopt pmma material, and electrode material adopts graphite electrode material, is provided with electrode groove, fixed electrode in electrolytic cell; Pending pedotheque is placed with in groove.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105290100A (en) * | 2015-11-23 | 2016-02-03 | 河海大学 | Method for remedying facility continuous cropping acidized soil through microorganism-electric combination |
| CN105396871A (en) * | 2015-12-21 | 2016-03-16 | 中国科学院沈阳应用生态研究所 | High-efficiency synergetic remediation method for hydrocarbon contaminated soil |
| CN105424773A (en) * | 2015-11-05 | 2016-03-23 | 中国石油天然气股份有限公司 | Electrolytic tank for soil corrosion electrochemical experiment |
| CN105466751A (en) * | 2016-01-04 | 2016-04-06 | 核工业北京地质研究院 | Device for gathering metal ions in soil and application method of device |
| CN105880276A (en) * | 2016-06-27 | 2016-08-24 | 上海大学 | Repair method of cadmium and lead polluted soil and electric-microorganism united permeable reaction wall repair device |
| CN106140804A (en) * | 2016-07-18 | 2016-11-23 | 中国科学院沈阳应用生态研究所 | One utilizes Dissolved Organic Matter to improve the electronic repairing method of microorganism of organic polluted soil |
| CN106190131A (en) * | 2016-07-18 | 2016-12-07 | 中国科学院沈阳应用生态研究所 | A kind of add electrolyte preparations and replenishment method thereof for electronic microorganism remediation |
| CN106881345A (en) * | 2017-03-14 | 2017-06-23 | 上海大学 | The EK PRB combined remediation methods and its device of heavy metal Cr (VI) contaminated soil |
| CN107030101A (en) * | 2017-03-28 | 2017-08-11 | 上海大学 | A kind of method of utilization microbial association EK PRB restoration of soil polluted by heavy metal |
| CN107413837A (en) * | 2017-09-04 | 2017-12-01 | 西南石油大学 | Device and the application of oil heavy-metal composite pollution soil are repaired with electronic microbial association |
| CN108865880A (en) * | 2018-06-25 | 2018-11-23 | 中南林业科技大学 | A kind of screening has the device and method of the electric heavy metal resistance microorganism for moving ability |
| CN109759437A (en) * | 2019-01-21 | 2019-05-17 | 胡鹏程 | A kind of chemical pollution soil remediation construction method and constructing device |
| CN112658028A (en) * | 2020-12-30 | 2021-04-16 | 森特士兴集团股份有限公司 | Electrochemical-microbial coupling repair system and application thereof |
| CN112692051A (en) * | 2021-01-25 | 2021-04-23 | 明志科技大学 | Method and equipment for removing pollutants by using beneficial microorganism mixed bacterial liquid |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424773A (en) * | 2015-11-05 | 2016-03-23 | 中国石油天然气股份有限公司 | Electrolytic tank for soil corrosion electrochemical experiment |
| CN105290100A (en) * | 2015-11-23 | 2016-02-03 | 河海大学 | Method for remedying facility continuous cropping acidized soil through microorganism-electric combination |
| CN105290100B (en) * | 2015-11-23 | 2018-08-21 | 河海大学 | A kind of method of the electronic joint restoration facilities continuous cropping acidified soil of microorganism- |
| CN105396871A (en) * | 2015-12-21 | 2016-03-16 | 中国科学院沈阳应用生态研究所 | High-efficiency synergetic remediation method for hydrocarbon contaminated soil |
| CN105466751A (en) * | 2016-01-04 | 2016-04-06 | 核工业北京地质研究院 | Device for gathering metal ions in soil and application method of device |
| CN105880276A (en) * | 2016-06-27 | 2016-08-24 | 上海大学 | Repair method of cadmium and lead polluted soil and electric-microorganism united permeable reaction wall repair device |
| CN106190131A (en) * | 2016-07-18 | 2016-12-07 | 中国科学院沈阳应用生态研究所 | A kind of add electrolyte preparations and replenishment method thereof for electronic microorganism remediation |
| CN106140804A (en) * | 2016-07-18 | 2016-11-23 | 中国科学院沈阳应用生态研究所 | One utilizes Dissolved Organic Matter to improve the electronic repairing method of microorganism of organic polluted soil |
| CN106881345A (en) * | 2017-03-14 | 2017-06-23 | 上海大学 | The EK PRB combined remediation methods and its device of heavy metal Cr (VI) contaminated soil |
| CN107030101A (en) * | 2017-03-28 | 2017-08-11 | 上海大学 | A kind of method of utilization microbial association EK PRB restoration of soil polluted by heavy metal |
| CN107413837A (en) * | 2017-09-04 | 2017-12-01 | 西南石油大学 | Device and the application of oil heavy-metal composite pollution soil are repaired with electronic microbial association |
| CN108865880A (en) * | 2018-06-25 | 2018-11-23 | 中南林业科技大学 | A kind of screening has the device and method of the electric heavy metal resistance microorganism for moving ability |
| CN109759437A (en) * | 2019-01-21 | 2019-05-17 | 胡鹏程 | A kind of chemical pollution soil remediation construction method and constructing device |
| CN112658028A (en) * | 2020-12-30 | 2021-04-16 | 森特士兴集团股份有限公司 | Electrochemical-microbial coupling repair system and application thereof |
| CN112692051A (en) * | 2021-01-25 | 2021-04-23 | 明志科技大学 | Method and equipment for removing pollutants by using beneficial microorganism mixed bacterial liquid |
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