CN113304076A

CN113304076A - Composition and preparation for brightening skin color and preparation method and application thereof

Info

Publication number: CN113304076A
Application number: CN202110558401.9A
Authority: CN
Inventors: 张立国; 刘艳君; 潘宇
Original assignee: Harbin Beixing Pharmaceutical Co ltd
Current assignee: Harbin Fuerjia Technology Co ltd
Priority date: 2021-05-21
Filing date: 2021-05-21
Publication date: 2021-08-27
Anticipated expiration: 2041-05-21
Also published as: CN113304076B

Abstract

The invention provides the use of glucosyl rutin and bioflavonoids in any of 1) inhibiting the discoloration of astaxanthin; 2) the inactivation of astaxanthin is inhibited; 3) protecting astaxanthin; 4) the shelf life of cosmetics containing astaxanthin is prolonged. Accelerated experiments prove that glucosyl rutin and bioflavonoid can be synergized under a specific ratio, and astaxanthin is protected from being stable and fadeless at relatively high temperature and under illumination for 90 days at most. Under the general environment, the stable state of more than 2 years can be achieved. The glucosyl rutin and the bioflavonoid are natural plant extracts, and experiments prove that the glucosyl rutin and the bioflavonoid can be used for stabilizing natural pigments and enhancing the photostability of the astaxanthin when being added into a cosmetic formula, and in addition, the glucosyl rutin and the bioflavonoid can protect skin damage caused by UV and inhibit skin protein glycosylation to resist skin aging.

Description

Composition and preparation for brightening skin color and preparation method and application thereof

Technical Field

The invention relates to the research field of haematococcus pluvialis, and in particular relates to a composition and a preparation for brightening skin color, and a preparation method and application thereof.

Background

The human body is continuously contacted with the outside, free radicals are generated in the human body due to factors such as human respiration, working pressure, light pollution, air pollution and the like, and the condition that the human skin is aged is mostly related to the superscript of the free radicals of the human body. The antioxidant cosmetics can effectively remove excessive free radicals, keep the skin elasticity and delay the skin aging, which is an important research and development direction of modern cosmetic enterprises. Astaxanthin is used in cosmetics to eliminate free radicals and brighten skin. Astaxanthin is a natural carotenoid, belongs to a terpene unsaturated compound, contains a conjugated polyene structure in a molecular structure, is extremely unstable in chemical property, and is easily damaged by the action of substances such as light, heat, oxides, free radicals and the like to influence the service life of the astaxanthin.

Astaxanthin in haematococcus pluvialis is one kind of carotenoid, namely 3,3 '-dihydroxy-4, 4' -diketone-beta, beta '-carotene, the structure is mainly 3S-3' S type, the astaxanthin is a terpene unsaturated compound, two beta-ionone rings and 11 conjugated double bonds are arranged in the molecular structure, and the special molecular structure of the multi-conjugated double bond terpene unsaturated compound has stronger activity, is easy to lose and accept electrons, and generates an oxidation free radical reaction and a lipid peroxidation reaction. The oxidation free radical reaction and the lipid peroxidation play important roles in the metabolism process in human cells, and the oxidation free radical reaction and the lipid peroxidation are in a coordinated and dynamic balance state under normal conditions, so that a plurality of physiological and biochemical reactions and immune reactions in vivo are maintained. Lipid peroxidation occurs when there is an imbalance between coordination and homeostasis. The natural astaxanthin has effects of inhibiting or reducing lipid peroxidation, and can protect phosphatidylcholine lipid from oxidation. Also the highest-grade products of carotenoid synthesis found so far, have strong antioxidant properties in nature. The special molecular structure of natural astaxanthin extracted from Haematococcus pluvialis can penetrate through the outer wall of human cell, directly remove oxygen free radical in cell, enhance cell regeneration capability, maintain human body function balance, reduce accumulation of senescent cell, and protect cell and DNA health from inside to outside.

The astaxanthin is generally protected by physical means such as a method of keeping out of the sun and avoiding high temperature; chemical means such as protection by addition of chemical sunscreens. The physical mode has special requirements on the packaging, storage and transportation of the product, the cost investment of the product can be correspondingly increased, the chemical sun-screening agents such as benzotriazole sodium styrene-butadiene phenolsulfonate, 4-methylbenzylidene camphor, ethylhexyl triazone and the like can accelerate skin aging and skin irritation due to free radical risk when being irradiated by light while protecting the product, and some chemical sun-screening agents have pollution risk to water and are not beneficial to environmental protection.

Disclosure of Invention

Therefore, the technical problem to be solved by the present invention is to overcome the defect that haematococcus pluvialis is easily discolored (wherein astaxanthin is easily inactivated) when added into cosmetics in the prior art, thereby providing the use of glucosyl rutin and bioflavonoids for inhibiting the discoloration of astaxanthin, a composition for lightening skin color, and a preparation for lightening skin color.

The application of glucosyl rutin and bioflavonoid in any one of the following applications,

1) the fading of astaxanthin is inhibited;

2) the inactivation of astaxanthin is inhibited;

3) protecting astaxanthin;

4) the shelf life of cosmetics containing astaxanthin is prolonged.

Optionally, the mass part ratio of glucosyl rutin to bioflavonoid is 1 (0.5-1.5); optionally, 1:1.

A composition for lightening skin color comprises component 1, wherein component 1 comprises Haematococcus pluvialis extract, glucosylrutin, and bioflavonoids.

Optionally, the mass part ratio of the haematococcus pluvialis extract, glucosyl rutin and bioflavonoid is 1 (0.8-1.5) to 0.5-1.5.

Optionally, the mass part ratio of the haematococcus pluvialis extract, glucosyl rutin and bioflavonoid is 1:0.8:0.5, 1:1:0.8, 1:1:1, 1:1.5:1 or 1:1.5: 1.5.

Optionally, the composition further comprises component 2; the component 2 comprises yeast/rice fermentation product filtrate and bacillus/soybean fermentation product extract.

Optionally, the mass part ratio of the yeast/rice fermentation product filtrate to the bacillus/soybean fermentation product extract is 2: 1.

optionally, the mass part ratio of the component 1 to the component 2 is 1 (1-12); optionally, the mass part ratio of the component 1 to the component 2 is 1: 10.

An external preparation for lightening skin color, comprising a composition for lightening skin color; optionally, the skin care product also comprises any one or more of a humectant, an antioxidant, a skin conditioner, a chelating agent, a solvent, a thickener, an emollient and an emulsifier; optionally, the humectant is selected from any one or more of butanediol, 1, 2-pentanediol, glycerin acrylate/acrylic acid copolymer, acetyl chitosamine, hydrolyzed sodium hyaluronate, betaine, beta-glucan and ethylhexylglycerin; the antioxidant is selected from one or more of tranexamic acid, ceramide 3 and vitamin E acetate; the skin conditioner is selected from one or more of glycosphingolipids, extract of Tremella fuciformis fruiting body, and glycosphingolipids; the chelating agent is EDTA disodium; the solvent is water; the thickening agent is acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer; the emollient is jojoba wax PEG-120 esters; the emulsifier is selected from one or more of polyacrylamide, C13-14 isoparaffin, laureth-7, and PEG-40 hydrogenated castor oil; optionally, the formulation is a serum, a mask or a lotion.

The use of the above composition, or the above formulation, in any one or more of the following; 1) improving dullness; 2) improving dark yellow; 3) improving black spots; 4) lightening the skin color; 5) lightening and color-sinking; 6) repairing skin.

Specific mask formulations are shown in the following table.

TABLE 1 Haematococcus pluvialis mask prescription (total amount 1000g)

Preparation method of haematococcus pluvialis mask

1. Dissolving the EDTA disodium in the A into water, adding the rest components, and fully stirring until the solution is clear and transparent;

2. dissolving glucosyl rutin and bioflavonoid in the B into water, stirring uniformly, adding other components, and stirring uniformly for later use;

3. dispersing the extract of the tremella fuciformis in the C in water, and stirring to dissolve the extract to fully swell the extract for later use;

4. dispersing the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer in the D into water, stirring and dissolving to fully swell the polymer for later use;

5. b, C, D is added into the A, after being fully stirred and mixed evenly, 1mol/L sodium hydroxide is added to adjust the pH value to 5.8 to 6.4, the residual water quantity (adjusted according to the actual required supplementary quantity) is supplemented to the total quantity of the prescription, and the mixture is fully mixed evenly;

6. and (5) vacuum exhausting and filling according to actual needs.

The specific essence formula is shown in the following table.

TABLE 2 Haematococcus pluvialis essence recipe (total amount is 1000g)

The preparation method of the haematococcus pluvialis essence comprises the following steps:

3. dispersing the sodium hyaluronate in the C in water, stirring and dissolving to fully swell the sodium hyaluronate for later use;

5. b, C, D, E is added into A, homogenized for 3-5 minutes, added with 1mol/L sodium hydroxide to adjust pH to 5.8-6.4, and the residual water amount (adjusted according to the actual required supplement amount) is supplemented to the total amount of the prescription, and fully and uniformly mixed;

6. and (5) vacuum exhausting and filling according to actual needs.

Specifically, the emulsion formulations are shown in the following table.

TABLE 3 Haematococcus pluvialis emulsion formula (total amount is 1000g)

The preparation method of the emulsion comprises the following steps:

3. stirring and mixing the components in the step C uniformly for later use;

4. pre-dispersing ceramide 3 in D in water, and stirring to obtain uniform milky white solution (which can be dissolved in water bath at below 60 deg.C);

5. b, C is added into A, after homogenizing for 5-10 minutes, D is added, and then homogenizing is carried out for 3-5 minutes, the residual water amount is supplemented (the supplement amount is adjusted according to the actual requirement) to the total amount of the prescription, and the mixture is fully and uniformly mixed; 6. vacuum exhausting, aging for 4-6 hr, and filling as required.

The technical scheme of the invention has the following advantages:

1. the invention provides application of glucosyl rutin and bioflavonoid in any one of the following aspects, which can inhibit fading of astaxanthin; the inactivation of astaxanthin is inhibited; protecting astaxanthin; the shelf life of cosmetics containing astaxanthin is prolonged. Accelerated experiments prove that glucosyl rutin and bioflavonoid can be synergized under a specific ratio, and astaxanthin is protected from being stable and fadeless at relatively high temperature and under illumination for 90 days at most. Under the general environment, the stable state of more than 2 years can be achieved. The glucosyl rutin and the bioflavonoid are natural plant extracts, and experiments prove that the glucosyl rutin and the bioflavonoid can be used for stabilizing natural pigments and enhancing the photostability of the astaxanthin when being added into a cosmetic formula, and in addition, the glucosyl rutin and the bioflavonoid can protect skin damage caused by UV and inhibit skin protein glycosylation to resist skin aging.

2. The invention provides an external preparation for brightening skin color, which can obviously improve dull and dark yellow skin, improve black spots and improve skin brightness.

Detailed Description

The following examples are provided to further understand the present invention, not to limit the scope of the present invention, but to provide the best mode, not to limit the content and the protection scope of the present invention, and any product similar or similar to the present invention, which is obtained by combining the present invention with other prior art features, falls within the protection scope of the present invention.

The examples do not show the specific experimental steps or conditions, and can be performed according to the conventional experimental steps described in the literature in the field. The reagents or instruments used are not indicated by manufacturers, and are all conventional reagent products which can be obtained commercially.

Example 1 Effect test of Haematococcus pluvialis composition on skin lightening

First, the protective effect of glucosyl rutin and bioflavonoid on haematococcus pluvialis extract is investigated

Astaxanthin in Haematococcus pluvialis extract has red color when it has antioxidant activity, and when the astaxanthin is destroyed by external temperature, illumination, etc., it loses original red color and turns into colorless transparent state.

Based on the property of astaxanthin, this example performed a set of accelerated tests to examine the protective effect of adding glucosyl rutin and bioflavonoids on astaxanthin in Haematococcus pluvialis extract at 37 ℃. + -. 2 ℃ under light conditions, wherein the test observation time is 5 days, 10 days, 15 days, 30 days, 60 days, and 90 days, respectively, and the test results are shown in Table 4 below:

TABLE 4 accelerated test results of the protective effect of glucosylrutin and bioflavonoids on H.pluvialis extracts

As can be seen from Table 4, glucosylrutin and bioflavonoids have protective effect on astaxanthin in Haematococcus pluvialis extract. After 90 days of accelerated test investigation, when the haematococcus pluvialis extract in the formula: glucosyl rutin: when the ratio of the bioflavonoids reaches 1:1:1, the glucosyl rutin and the bioflavonoids can protect the astaxanthin from being stable and not fading at relatively high temperature and under illumination. This example also considers the protective effect of higher proportion of glucosylrutin and bioflavonoids on Haematococcus pluvialis extract, and the test result is also that astaxanthin can be stably protected, but considering the cost of raw materials in practical application, this example finally selects Haematococcus pluvialis extract: glucosyl rutin: the ratio of bioflavonoids is 1:1: 1.

And secondly, a declared evaluation test is carried out on the effects of brightening the skin color, lightening the color and settling and repairing the skin by considering the proportion of the component 1 (haematococcus pluvialis extract, glucosylrutin and bioflavonoids) to the component 2 (yeast/rice fermentation product filtrate, bacillus/soybean fermentation product extract). The formula ratio of component 1 (haematococcus pluvialis extract + glucosylrutin + bioflavonoids 1:1:1) to component 2 (mass ratio of rice fermentation product filtrate + soybean fermentation product extract is 2:1) is shown in table 5. The yeast/rice fermentation product filtrate and the bacillus/soybean fermentation product are products of Huaxi organism company.

TABLE 5 different proportions of component 1 (Haematococcus pluvialis extract + glucosylrutin + bioflavonoids) and component 2 (Yeast/Rice fermentation product filtrate + Bacillus/Soybean fermentation product)

Note: the proportions are mass ratios.

In the embodiment, 60 volunteers of 30-50 years old have dull, dark yellow and black spots on the skin, and are randomly divided into 6 groups of 10 persons for continuous trial for 30 days, and the trial effect of each experimental group is evaluated in the aspects of improving the dull, dark yellow and black spots on the skin. The test results are shown in Table 6.

TABLE 6 test results of different ratios

The test results in the table show that the composition of the haematococcus pluvialis extract, the glucosylrutin, the bioflavonoid, the rice fermentation product filtrate and the soybean fermentation product extract can improve dull, dark yellow and dark spots of the skin, and when the ratio of the (haematococcus pluvialis extract, the glucosylrutin and the bioflavonoid) to the (rice fermentation product filtrate and the soybean fermentation product extract) reaches 1:10, the dull and dark yellow of the skin can be obviously improved, and the skin brightness is improved; with the increase of the proportion of the rice fermentation product filtrate to the soybean fermentation product extract, although the effect of improving skin black spots is improved, the effect of the rice fermentation product filtrate to the soybean fermentation product extract is not good in proportion of 1:10 in other aspects, the odor of the rice fermentation product filtrate and the soybean fermentation product extract is not good, and after the addition amount is increased, the odor of the product is influenced, so that the experience feeling of consumers is reduced.

Example 2 Haematococcus pluvialis mask formula and preparation process

The haematococcus pluvialis mask is formulated as shown in table 7 below.

TABLE 7 Haematococcus pluvialis mask prescription (1000g)

Preparation process

1. Dissolving the EDTA disodium in the A into water, adding the rest components in the A, and fully stirring until the solution is clear and transparent;

2. dissolving glucosyl rutin and bioflavonoid in B in water, stirring well, adding other components in B, and stirring well for use;

5. b, C, D is added into A, after fully stirring and mixing evenly, 1mol/L sodium hydroxide is added to adjust the pH value to 6.0, the residual water amount (adjusted according to the actual required supplement amount) is supplemented to the total amount of the prescription, and the mixture is fully mixed evenly.

6. And (5) vacuum exhausting and filling according to actual needs.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.

Claims

1. The application of glucosyl rutin and bioflavonoid in any one of the following applications,

1) the fading of astaxanthin is inhibited;

2) the inactivation of astaxanthin is inhibited;

3) protecting astaxanthin;

4) the shelf life of cosmetics containing astaxanthin is prolonged.

2. The application of claim 1, wherein the ratio of the glucosyl rutin to the bioflavonoid is 1 (0.5-1.5); optionally, 1:1.

3. A composition for lightening skin color comprises component 1, wherein component 1 comprises Haematococcus pluvialis extract, glucosylrutin, and bioflavonoids.

4. The composition as claimed in claim 3, wherein the weight ratio of the haematococcus pluvialis extract, glucosyl rutin and bioflavonoid is 1 (0.8-1.5) to (0.5-1.5).

5. The composition according to claim 3 or 4, wherein the ratio of the haematococcus pluvialis extract, glucosyl rutin and bioflavonoid in parts by weight is 1:0.8:0.5, 1:1:0.8, 1:1:1, 1:1.5:1 or 1:1.5: 1.5.

6. The composition of any one of claims 3-5, wherein the composition further comprises component 2; the component 2 comprises yeast/rice fermentation product filtrate and bacillus/soybean fermentation product extract.

7. The composition according to any one of claims 3 to 6, wherein the ratio of yeast/rice fermentation product filtrate to bacillus/soybean fermentation product extract in parts by mass is 2: 1.

8. the composition according to any one of claims 2 to 7, wherein the mass part ratio of the component 1 to the component 2 is 1 (1-12); optionally, the mass part ratio of the component 1 to the component 2 is 1: 10.

9. An external preparation for lightening skin color, comprising the composition for lightening skin color of any one of claims 3 to 8; optionally, the skin care product also comprises any one or more of a humectant, an antioxidant, a skin conditioner, a chelating agent, a solvent, a thickener, an emollient and an emulsifier; optionally, the humectant is selected from any one or more of butanediol, 1, 2-pentanediol, glycerin acrylate/acrylic acid copolymer, acetyl chitosamine, hydrolyzed sodium hyaluronate, betaine, beta-glucan and ethylhexylglycerin; the antioxidant is selected from one or more of tranexamic acid, ceramide 3 and vitamin E acetate; the skin conditioner is selected from one or more of glycosphingolipids, extract of Tremella fuciformis fruiting body, and glycosphingolipids; the chelating agent is EDTA disodium; the solvent is water; the thickening agent is acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer; the emollient is jojoba wax PEG-120 esters; the emulsifier is selected from one or more of polyacrylamide, C13-14 isoparaffin, laureth-7, and PEG-40 hydrogenated castor oil; optionally, the formulation is a serum, a mask or a lotion.

10. Use of a composition according to any one of claims 3 to 8, or a formulation according to claim 9, in any one or more of the following;

1) improving dullness;

2) improving dark yellow;

3) improving black spots;

4) lightening the skin color;

5) lightening and color-sinking;

6) repairing skin.