CN113303399A - Plant compound extract and preparation method thereof - Google Patents

Abstract

The invention discloses a plant compound extract which can replace antibiotics to ensure the healthy growth of animals. Comprises the following components: 2-10 parts of paris polyphylla, 10-15 parts of lophatherum gracile, 10-15 parts of rhus chinensis, 10-15 parts of pteridophyte, 10-15 parts of houttuynia cordata, 18-20 parts of sweet potato and 25-30 parts of dried kelp.

Description

Plant compound extract and preparation method thereof

Technical Field

The invention relates to the field of plant extracts, in particular to a plant compound extract and a preparation method thereof.

Background

In the cultivation of livestock, aquatic products and the like, antibiotics are widely used in the process of epidemic disease prevention and treatment, so that the immunity of animals is reduced, endogenous infection is caused, and the drug resistance of the animals is enhanced; the environment also causes the quality to be reduced due to the drug residue of antibiotics; meanwhile, the feed also threatens human health and causes negative effects, so that all countries are in the feed for resisting antibiotics and chemosynthetic drugs by appointed laws and regulations.

In the work scheme of animal antibacterial use reduction action trial points (2018-2021) issued in rural agricultural departments, it is pointed out that by reducing the use of additives such as antibacterial in 3 years, the problems of drug residues and animal bacteria resistance should be effectively controlled.

Therefore, those skilled in the art should develop alternative products to replace antibiotics, thereby improving the physical quality of animals and reducing the environmental pollution.

Disclosure of Invention

The invention discloses a plant compound extract which can replace antibiotics to ensure the healthy growth of animals.

The invention provides a plant compound extract, which comprises the following components: 2-10 parts of paris polyphylla, 10-15 parts of lophatherum gracile, 10-15 parts of rhus chinensis, 10-15 parts of pteridophyte, 10-15 parts of houttuynia cordata, 18-20 parts of sweet potato and 25-30 parts of dried kelp.

Preferably, the composition is: 5 parts of paris polyphylla, 10 parts of lophatherum gracile, 15 parts of rhus chinensis, 10 parts of pteridophyte, 10 parts of houttuynia cordata, 20 parts of sweet potato and 30 parts of dried kelp.

Preferably, the sweet potatoes are fresh sweet potatoes.

The invention also provides a preparation method of the plant compound extract, which comprises the following steps:

(1) taking the components of the plant compound extract in the claims 1-3 as raw materials: root of Rhus chinensis Mill, herb of Paris polyphylla Smith, fern, herb of Lophatherum gracile Smith, herba Houttuyniae, sweet potato, and dried herba Zosterae Marinae;

(2) cleaning raw materials, drying in the shade, pulverizing, and mixing;

(3) steaming at 100-;

(4) sterilizing the stock solution, adsorbing with bamboo charcoal for 7-15 days, drying bamboo charcoal in the shade, grinding, separating bamboo charcoal to obtain the final product, and packaging.

Preferably, the bamboo charcoal is massive bamboo charcoal.

Preferably, the kelp is a dried kelp extract, and the extraction method comprises the following steps:

putting the dried kelp raw material into a closed container, completely freezing at the temperature of minus 30-minus 20 ℃, and repeating for 2-3 times when the kelp raw material is completely thawed at the temperature of 25-30 ℃;

drying the kelp: purified water is prepared according to the weight portion ratio of 1:10, and the pH value is 9: heating in water bath to 40-45 deg.C, maintaining the temperature for 1 hr, and stirring for 10-15 min; heating in water bath, maintaining the temperature, and stirring for 3-8 times;

adding alginate lyase and 2% complex enzyme according to 0.1% of the weight of the dry kelp, stirring for 3-8 hours at the temperature of 40-45 ℃ at the speed of 300-4000 r/min, centrifuging for 10-20 minutes at the speed of 3000-4000r/min, and performing solid-liquid separation to obtain a supernatant which is the kelp extract;

wherein the parameter of the alginate lyase is 1000U/mL;

the compound enzyme is pectinase, cellulase and protease, the ratio of the pectinase to the cellulase to the protease is 1:1:1, and the parameter is 2 ten thousand U/mL.

The plant compound extract provided by the invention comprises paris polyphylla, lophatherum gracile, rhus chinensis, fern, houttuynia cordata, sweet potatoes and dried kelp, and the natural extract is obtained by adopting the extraction method, and the components contain various plant polysaccharides, flavonoids, saponins, alkaloids, organic acids, vitamins and natural minerals, so that the extract has double functions of nutrition and medicine, can promote the glycometabolism of the organism, promote the synthesis of proteins and enzymes, increase the antibody titer of the organism and stimulate the gonadal development, and has the functions of sterilization, bacteriostasis, regulation of the immunity of the organism and nonspecific antibiosis.

Furthermore, the plant raw materials are easy to obtain, and the extraction method is easy to popularize and meets the production requirements.

Drawings

FIG. 1 shows the results of a plant compound extract and its preparation method and the effect of the compound plant extract on the expression of immune factors and tight junction proteins in the jejunum (A), duodenum (B) and ileum (C) of weaned pigs in analysis.

Detailed Description

The invention discloses a plant compound extract and a preparation method thereof, which can replace antibiotics to ensure the healthy growth of animals.

The technical solutions in the embodiments of the present invention are described in detail below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a plant compound extract, which comprises the following components: 2-10 parts of paris polyphylla, 10-15 parts of lophatherum gracile, 10-15 parts of rhus chinensis, 10-15 parts of pteridophyte, 10-15 parts of houttuynia cordata, 18-20 parts of sweet potato and 25-30 parts of dried kelp.

Preferably, the composition is: 5 parts of paris polyphylla, 10 parts of lophatherum gracile, 15 parts of rhus chinensis, 10 parts of pteridophyte, 10 parts of houttuynia cordata, 20 parts of sweet potato and 30 parts of dried kelp.

Preferably, the sweet potatoes are fresh sweet potatoes.

The invention also provides a preparation method of the plant compound extract, which comprises the following steps:

(1) taking the components of the plant compound extract in the claims 1-3 as raw materials: root of Rhus chinensis Mill, herb of Paris polyphylla Smith, fern, herb of Lophatherum gracile Smith, herba Houttuyniae, sweet potato, and dried herba Zosterae Marinae;

(2) cleaning raw materials, drying in the shade, pulverizing, and mixing;

(3) steaming at 100-;

(4) sterilizing the stock solution, adsorbing with bamboo charcoal for 7-15 days, drying bamboo charcoal in the shade, grinding, separating bamboo charcoal to obtain the final product, and packaging.

Preferably, the kelp is a dried kelp extract, and the extraction method comprises the following steps:

putting the dried kelp raw material into a closed container, completely freezing at the temperature of minus 30-minus 20 ℃, and repeating for 2-3 times when the kelp raw material is completely thawed at the temperature of 25-30 ℃;

drying the kelp: purified water is prepared according to the weight portion ratio of 1:10, and the pH value is 9: heating in water bath to 40-45 deg.C, maintaining the temperature for 1 hr, and stirring for 10-15 min; heating in water bath, maintaining the temperature, and stirring for 3-8 times;

adding alginate lyase and 2% complex enzyme according to 0.1% of the weight of the dry kelp, stirring for 3-8 hours at the temperature of 40-45 ℃ at the speed of 300-4000 r/min, centrifuging for 10-20 minutes at the speed of 3000-4000r/min, and performing solid-liquid separation to obtain a supernatant which is the kelp extract;

wherein the parameter of the alginate lyase is 1000U/mL;

the compound enzyme is pectinase, cellulase and protease, the ratio of the pectinase to the cellulase to the protease is 1:1:1, and the parameter is 2 ten thousand U/mL.

The extraction method reduces cell wall activity by quick freezing and quick warming, forces the kelp cell to be in a state of easy damage, and directly breaks partial cell products, thereby performing water bath and stirring to effectively improve extraction amount.

The method comprises the following steps:

(1) taking the following components: cleaning Paris polyphylla whole grass, lophatherum gracile whole grass leaves, rhus chinensis roots, ferns, houttuynia cordata, sweet potatoes and dry kelp, drying in the shade in a sterile manner, and crushing the components for later use;

(2) mixing the components according to the parts by weight, steaming at the temperature of 100-120 ℃, and naturally cooling to normal temperature;

(3) adding red yeast rice after steaming, and fermenting for 7-15 days to obtain a fermented semi-finished product;

the weight parts of the addition amount of the red yeast rice are as follows: the components (Paris polyphylla whole grass, lophatherum gracile whole grass leaf, Salicomia Herbacea root, fern, houttuynia cordata, sweet potato and dried kelp): red yeast rice 100: 2-5;

(4) filtering the semi-finished product to remove residues to obtain stock solution, and sterilizing;

(5) adsorbing the sterilized stock solution with bamboo charcoal blocks for 7-15 days, air drying the bamboo charcoal at low temperature, and grinding to obtain bamboo charcoal powder and stock solution mixture;

(6) separating bamboo charcoal to obtain plant compound extract;

wherein, paris polyphylla and the whole grass thereof are plants of paris of liliaceae, and the chemical components of the paris polyphylla are rich in paris polyphylla saponin A, paris polyphylla saponin A, B, paris polyphylla saponin C, D, E, F, G, H, paris polyphylla sterone (parister-one), creatinine (creatinine), amino acid and the like. Has effects in removing toxic materials, resisting cancer, relieving swelling and pain, clearing away heat, arresting convulsion, relieving cough, and relieving asthma.

Experiments prove that the paris polyphylla has the antibacterial effect that the water infusion and the decoction have the inhibition effect on typhoid bacillus, paratyphoid bacillus A, shigella and shigella flexneri in vitro, and the biological water infusion has stronger antibacterial effect than the decoction. The alcohol extract has obvious sedative and analgesic effects.

Paris polyphylla has antibacterial effect to Staphylococcus aureus, hemolytic streptococcus, meningococcus, dysentery bacillus, typhoid bacillus, paratyphoid bacillus, Escherichia coli and Pseudomonas aeruginosa.

It has obvious inhibition effect on dysentery bacillus, serratia marcescens, colibacillus, sensitive and drug-resistant staphylococcus aureus in vitro; it has inhibitory effect on influenza A virus and influenza Asian A virus, and has effect in killing leptospira, and is not suitable for decoction of water with the same concentration. Can inhibit the growth of HeLa tumor strain and L929 tumor strain in vitro; in vivo experiments, the composition is effective on Ehrlich ascites tumor EAC; the total saponins can also inhibit the growth of H22 solid tumor.

The whole herb of paris polyphylla is the part with the strongest effect in paris polyphylla and is the part which is most convenient to prepare.

Lophatherum gracile and the whole herb thereof are perennial herbs of Lophatherum of Gramineae. The stem and the leaf of the plant contain triterpene compounds: arundo donax, imperatorin, taraxasterol and friedelin; the aerial part contains phenolic components, amino acids, organic acids, and saccharides.

The folium Bambusae contains abundant flavonoids, biological polysaccharides and other effective components, such as phenolic acid compounds, anthraquinone compounds, terpene lactone, special amino acids, and trace elements such as active manganese, zinc, and selenium. The functional factors contained in the lophatherum gracile leaves mainly comprise flavone glycoside and coumarin lactone.

The active ingredients of the lophatherum gracile extract comprise flavone, phenolic acid, anthraquinone, lactone, polysaccharide, amino acid, trace elements and the like, and the lophatherum gracile extract has excellent effects of resisting free radicals, resisting oxidation, resisting aging, resisting fatigue, reducing blood fat, preventing cardiovascular and cerebrovascular diseases, protecting liver, expanding capillary vessels, dredging microcirculation, activating brain, promoting memory, improving sleep, resisting cancers, beautifying skin and the like.

The bamboo leaf flavone has small molecular weight and can be absorbed by human body, can effectively remove free radical and ester peroxide and block the synthesis of strong carcinogen N-nitrous acid amine compound, can obviously improve the immunity of the organism, resist diseases, regulate blood pressure, reduce blood fat and improve cerebral ischemia, and simultaneously has high-quality active ingredients and biological effects of resisting fatigue and aging.

The modern pharmacological experiments prove that the lophatherum gracile has the effects of relieving fever, promoting urination, inhibiting bacteria, resisting tumors and increasing blood sugar. Can obviously increase the chloride excretion in urine while promoting urination. The specific antibacterial effect is that the decoction has an inhibitory effect on staphylococcus aureus and hemolytic streptococcus.

Rhus chinensis Mill root is root of Rhus chinensis Mill of Anacardiaceae. Its main functions are clearing away heat and toxic material, removing blood stasis and stopping bleeding. Has effects in expelling pathogenic wind, eliminating dampness, relieving swelling, and softening hard masses. In terms of pharmacology, it has the effects of increasing coronary blood flow, resisting bacteria and viruses.

The main components of the composition comprise: the triterpenes are 9 compounds, and are divided into dammarane type, friedelane type, and lupane type, wherein hemiptera rhus chinensis lactone and hemiptera rhus chinensis acid are separated from bark of Rogowski drumstick. 3-keto-6-beta-hydroxy-oleanane-12-alkene-28-acid, 3-keto-6-beta-hydroxy-oleanane-18-alkene-28-acid, betulin and betulonic acid are also separated from the rhus chinensis root respectively.

The flavonoids comprise 10 compounds, such as quercetin, fisetin, 3', 4', 7-trihydroxyflavone, dihydrofisetin, rhus chinensis chalcone A, rhaponticin, rhus chinensis biflavone A, etc.

The tannin and the phenolic acid are separated from the rhus chinensis to obtain 13 compounds: gallic acid, ethyl gallate, methyl shikimate, 3, 5-dihydroxytoluene, 3-hydroxy-5-methylphenol-1-o- β -D- (6 '-gallic acid) glucofuranoside, 3-hydroxy-5-methylphenol-1-o- β -D- (6' -gallic acid) glucose), 3, 4, 5-trimethoxyphenyl-1-o- β -D-glucopyranose), 2[2, 3 dihydroxy-1- (4-hydroxyphenyl) propyl ] -5-methylbenzene-1, 3-diol, pentagalloyl glucose, rhamnolactone A, and the like.

Polysaccharides and other polysaccharide-like substances are important bioactive substances in animals and plants. The rhus chinensis root polysaccharide mainly comprises galactose, galacturonic acid, glucuronic acid and the like.

The effective components of the rhus chinensis root are as follows: contains tannin 50-70%, and most of tannin 80% in some regions, mainly galloyl glucose, free gallic acid, fat, resin, and starch; and organic acids including malic acid, tartaric acid, citric acid, and the like.

Ferns, also known as ferns, are a group of spore plants with the highest level of evolution. The fern contains various alkaloids, phenols, flavones, zizi and triterpenes, has physiological activity, and has antibacterial and bacteriostatic effects.

The houttuynia cordata is a herbal medicine recorded in Chinese pharmacopoeia, and the character of the herbal medicine is that the stem is in a flat cylinder shape, is twisted, has a length of 20-35 cm and a diameter of 0.2-0.3 cm; the surface is brownish yellow, has a plurality of longitudinal edges and obvious sections, and residual fibrous roots are arranged on the lower sections; is brittle and easy to break.

It is cold and cool in nature and enters lung meridian. It has effects of clearing away heat and toxic materials, relieving swelling, treating sore, inducing diuresis, removing dampness, clearing away heat, relieving dysentery, invigorating stomach, and resolving food stagnation, and can be used for treating lung abscess, pyocutaneous disease, hemorrhoid hematochezia, and spleen and stomach heat accumulation due to excess heat, heat toxin, dampness pathogen, and disease heat. Modern pharmacological experiments show that the product has antibacterial, antiviral, immunity improving, and diuretic effects.

The pharmacology comprises the following steps: in the aspect of enhancing immunity, enhancing phagocytic capacity of WBC and improving serum properdin, and when the medicine is used for treating chronic tracheitis, the synthesized houttuynin can obviously improve the phagocytic capacity of WBC of a patient on staphylococcus albus and obviously improve the serum properdin. Has antibacterial effect, and can inhibit various microorganisms (especially yeast and mold), hemolytic streptococcus, Staphylococcus aureus, Bacillus influenzae, Catalpus, and pneumococcus. It also has effect on Escherichia coli, Bacillus dysenteriae, and Bacillus typhonii, and the synthetic decanoyl acetaldehyde sodium bisulfite adduct is called synthetic houttuynin. In the aspect of virus action, the houttuynia cordata has an inhibition effect on influenza Asia A virus, influenza virus and influenza virus. In the function of diuresis, the blood capillary can be expanded, and the blood flow and urine secretion are increased, so that the function of diuresis is achieved. On the immune system, the houttuynine sodium bisulfite can improve the phagocytic function of white blood cells of chronic tracheitis patients and improve the serum properdin level. In the anti-tumor effect, the mouse is injected with different doses of the new houttuynine sodium bisulfite at different time, the total number of cancer cells, the division index of the cancer cells and the abdominal water volume are all obviously reduced, and the cAMP level in the cancer cells is increased. In the aspect of anti-inflammatory action, the composition can obviously inhibit the thermal denaturation of human gamma-globulin in the presence of Cu + +, also has obvious inhibition effect on dye exudation of abdominal capillary vessels caused by HCA, and can obviously inhibit capillary hyperfunction in the early stage of inflammation.

The sweet potato, also called sweet potato, is rich and rich in nutrition, protein, fat, polysaccharide, phosphorus, calcium, potassium, carotene, vitamin A, vitamin C, vitamin E, vitamin B1, vitamin B2 and 8 kinds of amino acids.

The protein content, carotene content and vitamin A content of the fertilizer are 3-100 times different from those of similar crops.

Dried kelp is prepared by drying fresh kelp in the sun and extracting the extract of kelp by a certain means. The kelp is marine vegetables with rich nutritive value, is rich in algal polysaccharides, proteins, fat, vitamins and mineral substances, also contains growth regulating substances such as cytokinin, auxin and phenols, antibiotic substances, and also contains components such as iodine, potassium, calcium, iron, manganese, titanium and the like which cannot be compared with common terrestrial plants. Alginic acid, a polysaccharide carbohydrate extracted from kelp, is an important active substance.

A herba Zosterae Marinae extract is prepared from leaves of herba Zosterae Marinae of Laminariaceae by breaking cell wall by physical, chemical or biological technique, and extracting intracellular substances. The material contains large amount of seaweed polysaccharide, mannitol, amino acids, vitamins, minerals, phytohormones, betaine, etc. Has antioxidant, anticoagulant, antitumor, antibacterial, blood pressure lowering, and radioprotective effects.

In the aspect of traditional Chinese medicine, the primary kelp is a cold and cool substance, and a large amount of edible primary kelp can cause sweating due to deficiency of human bodies, and is particularly obvious in spleen and stomach, but the primary kelp contains abundant nutrient substances, so that the effective utilization of kelp nutrients is realized by extracting effective substances in the kelp, and meanwhile, the problem of cold and cool primary kelp discussed in the aspect of traditional Chinese medicine can be avoided.

The kelp extract has antioxidant effect, and the ethyl acetate extracted from the kelp extract has higher capability of removing various free radicals than that of an antioxidant 2, 6-di-tert-butyl and is dose-dependent; both the kelp polyphenol and the kelp polysaccharide have antioxidant activity.

The laminarin extract has an anti-tumor effect, and multiple experiments show that different doses of laminarin are used for intragastric administration to mice, and the results show that the laminarin can obviously enhance the T lymphocyte proliferation capacity of normal mice and immunosuppressed mice and can improve the activity of NK cells. The kelp polysaccharide sulfate with different concentrations is used for treating the HeLa cell, and the kelp polysaccharide sulfate can inhibit the growth of human cervical cancer cell lines and can regulate and control the expression of bc-l2 protein and NF-JB p65 to influence the apoptosis of the cervical cancer cell. When the antitumor activity of the crude extract of the kelp polyphenol is measured by an MTT method, the kelp polyphenol has certain inhibition effect on cancer cells such as A549, P388, BEL-7402, Hela and the like.

The kelp extract has antibacterial effect, and has obvious inhibiting effect on Bacillus subtilis, Escherichia coli, Tetracoccus tetragonorrhoeae and Staphylococcus aureus, and the diluted extract has inhibiting effect on strains.

The kelp extract has the function of reducing blood sugar, the polysaccharide substance of the kelp extract can obviously reduce the blood sugar and urea nitrogen of a diabetic mouse, increase the serum calcium and serum insulin content of the diabetic mouse, has obvious recovery function on islet injury caused by alloxan, and the kelp polysaccharide has the functions of reducing blood sugar and protecting islet cells of the diabetic mouse.

The Laminaria japonica extract has radiation protection effect, and laminarin has obvious improvement effect on sexual function of male rats with reproductive system damaged by ionizing radiation, and can shorten erection latent period and straddling latent period of penis. Daily exposure to ultraviolet radiation reduced the levels of malondialdehyde and hydrogen peroxide in the skin tissue of the high dose laminarin mice, and increased the levels of catalase, hydroxyproline, type I collagen mRNA, SO, glutathione peroxidase, and serum NO. Meanwhile, an ultramicro electron microscope shows that the MVEC cell membranes of the group of mice are complete, and the mitochondrial cristae is clear. The laminarin is shown to be capable of enhancing the oxidation resistance of the skin, regulating the synthesis of skin collagen and protecting skin MVEC cells.

The laminaria japonica aresch extract has the functions of resisting fatigue and anoxia, and the test mice fed with laminaria japonica aresch undergo continuous 10-day fatigue-resisting and closed anoxia tests, and the results show that the test mice have obviously prolonged heavy-load swimming time and survival time under normal-pressure anoxia, and obviously raised hemoglobin content, so that the laminaria japonica aresch polysaccharide has better anoxia-resisting effect and antifatigue effect. Through the blood gas analysis of the hypoxic mouse, the laminarin is proved to improve the utilization rate of the hypoxic mouse tissue to oxygen again.

The herba Zosterae Marinae extract has immunoregulation effect, and can be used for regulating immunity of mouse with hypoimmunity.

The kelp extract has anticoagulant and antithrombotic effects, and the laminarin can obviously prolong the blood Activated Partial Thrombin Time (APTT), Prothrombin Time (PT) and whole blood Coagulation Time (CT) of the domestic rabbit, and has obvious dose effect, which indicates that the kelp cell wall polysaccharide has certain anticoagulant effect. The fucoidan sulfate with low molecular weight in herba Zosterae Marinae can inhibit thrombosis by inhibiting tissue factor pathway in extrinsic coagulation pathway. Compared with the time of full occlusion of the blood vessel, the antithrombotic capacity is enhanced along with the increase of the molecular weight of the fucoidan sulfate.

Example 1

The preparation method of the plant compound extract comprises the following steps:

(1) taking the components of the plant compound extract as raw materials: 1500g of rhus chinensis root, 500g of whole herb of seven-leaf and one-branch flowers, 1000g of fern, 1000g of whole herb of lophatherum gracile, 1000g of houttuynia cordata, 2000g of sweet potato and 3000g of dried kelp;

(2) cleaning raw materials, drying in the shade, pulverizing, and mixing;

(3) steaming at 100-;

(4) sterilizing the stock solution, adsorbing with bamboo charcoal for 7-15 days, drying and grinding the bamboo charcoal in the shade, and separating the bamboo charcoal to obtain the final product.

Example 2

Substantially the same as in example 1, except that the step (1):

(1) taking the components of the plant compound extract as raw materials: 1500g of rhus chinensis root, 1000g of paris polyphylla whole plant, 1000g of fern, 1500g of lophatherum gracile whole plant, 1000g of houttuynia cordata, 2000g of sweet potato and 3000g of dried kelp.

The antibiotic (taking zinc oxide as an example) is replaced by the compound extract of the plant.

The problem of diarrhea easily occurs in the creep period of 2 weeks after weaning of piglets is that the milk is taken as the piglet of a nutrition source, the intestinal tract of the piglet is not completely developed in the weaning period, the change of the intake nutrition type, the change of the living environment and the like easily cause the influence of the piglet on nutrition, physiology and psychological stress sources to cause the problems of the type and the function disorder of intestinal tract strains, the barrier function disorder of the intestinal tract and the like, and further cause the production performance of the piglet caused by the diarrhea.

In the prior art, high-content zinc oxide is added into piglet feed to inhibit diarrhea and improve the production performance of piglets.

The health of piglets is affected by using zinc oxide in high dose for a long time, and residual zinc element is discharged along with excrement of piglets, so that environment pollution such as soil is caused, and adverse effects on plants or crops are caused; if zinc enters water resources, it will accumulate in the water supply chain, eventually inducing the generation of drug-resistant bacteria.

The experiment aims to research the influence of the compound plant extract on the diarrhea, the growth performance, the intestinal development and the organism immunity of the piglets in the weaning creep stage instead of zinc oxide.

The experimental method comprises the following steps: 24 first-weaned piglets of 28-day-old binary first-born weaned pigs with the weight of 8.2 +/-1.78 kg are randomly divided into two groups, each group is divided into six repetitions, each repetition is divided into two repetitions, and the test period is 14 days. The control group (CON group) added 2kg/t zinc oxide to the basal diet, and the compound plant extract group (CPE group) added 1kg/t compound plant extract to the basal diet instead of the zinc oxide in the control diet.

The results show that: after the compound plant extract is used for replacing zinc oxide, the weaned piglets do not show significant differences in apparent digestibility, diarrhea rate, growth performance and intestinal mucosa barrier (P is more than 0.05); compared with a CON group, the CPE group remarkably improves the lgA and lgG content (P is less than 0.01) in the serum of the weaned piglets and remarkably reduces the ALP and ALT content (P is less than 0.01); in terms of maintaining the intestinal morphology intact and healthy, the villus height and villus cryptic ratio of duodenum in CPE group were significantly higher than in CON group (P < 0.05).

And (4) conclusion: the results show that the composite plant extract can replace zinc oxide to achieve better effects in the aspects of diarrhea resistance, growth promotion, organism immunity improvement and intestinal mucosa barrier maintenance. Compared with zinc oxide, the compound plant extract with a certain amount is added into the daily ration, so that the immunity of weaned piglets can be improved more obviously, the oxidative stress caused by weaning can be relieved, the integrity and the health degree of intestinal tracts can be maintained, and the economic benefit can be improved.

Results and analysis

Influence of composite plant extract on growth performance of weaned piglets

As shown in the following Table 1, the results of the composite plant extract group and the control group have no significant difference in terms of average daily gain, feed intake, feed-to-feed ratio, diarrhea rate, etc. (P > 0.05). However, the material weight of the composite plant extract group is lower than that of the control group.

TABLE 1 Effect of composite plant extracts on growth Performance and diarrhea Rate of weaned piglets

Second, the influence of the compound plant extract on the apparent digestibility of the nutrient substances of the weaned pigs

As can be seen from table 2, after the composite plant extract is used to replace high-dose zinc oxide, the nutrient apparent digestibility of piglets is not significantly affected, and the crude protein and crude fat digestibility of piglets in the composite plant extract group is only numerically higher than that in the control group. In the content of zinc in the feces, the difference between the two groups is very significant (P < 0.01), and the zinc element discharged by the compound plant extract group is far less than that discharged by the control group.

TABLE 2 Effect of composite plant extracts on apparent digestibility and Zinc emission of weaned piglets

Wherein, the zinc content in the excrement is recorded as zinc element.

Third, the influence of the compound plant extract on the serum biochemical indexes of weaned pigs

As can be seen from Table 3, compared with the control group, the ALP and ALT activities of the weaned piglets in the compound plant extract group are remarkably reduced (P < 0.05), and the AST activity of the weaned piglets in the compound plant extract group is also reduced (P < 0.05 ≦ P < 0.10). The composite plant extract group showed no significant difference (P > 0.05) from the control group in TP and ALP activities.

TABLE 3 Effect of composite plant extracts on serum biochemical indicators of weaned piglets

Fourth, the influence of the compound plant extract on the serum immunity index of the weaned pig

As can be seen from Table 4, in terms of the immune index to the serum of the weaned pig, the contents of lgA and lgG in the serum of the piglet of the compound plant extract group are higher than those of the control group, and the difference reaches a significant level (P < 0.05), while the content of lgM in the serum of the piglet of the compound plant extract group is similar to that of the control group.

TABLE 4 influence of composite plant extracts on serum immune index of weaned piglets

Fifthly, the influence of the composite plant extract on the intestinal form of weaned pigs

As can be seen from Table 5, the intestinal morphology of the ileum of the piglets in the composite plant extract group is not significantly different from that of the control group in terms of the influence on the intestinal morphology of the weaned piglets (P is more than 0.05), and is only numerically superior; in the jejunum, the crypt depth of the piglets of the composite plant extract group is reduced (P is more than or equal to 0.05 and less than 0.10), and the crypt ratio is increased (P is more than or equal to 0.05 and less than 0.10); in duodenum, the crypt depth of piglets in the compound plant extract group was significantly lower than that in the control group (P < 0.05), and the villous cryptic ratio was significantly higher than that in the control group (P < 0.05).

TABLE 5 Effect of composite plant extracts on weaned piglet intestinal morphology

Sixth, the influence of the compound plant extract on the expression of intestinal immune factors and tight junction protein of weaned pigs

The effect of the compound plant extract on the content of immune factors in different intestinal sections of weaned pigs and the expression of tight junction proteins thereof is shown in figure 1 (figure 1 shows the effect of the compound plant extract on the expression of immune factors and tight junction proteins in jejunum (A), duodenum (B) and ileum (C) of the weaned pigs). No significant difference was observed in the change of the contents of the respective immune factors (P > 0.05) in the jejunum, duodenum and ileum, and the contents of type II interferon (gamma-IFN) were low in the jejunum and duodenum of piglets in the composite plant extract group. In the aspect of tight junction protein, the intestinal mucosa zonula occludens-1 (ZO-1), Occludin (Occludin) and transmembrane protein (Claudin) in the intestinal tract of the weaned piglets of the compound plant extract group and the control group have no significant difference (P is more than 0.05), and the expression levels of Claudin in the jejunum, Occludin in the duodenum, ZO-1 and Occludin in the ileum of the weaned piglets of the compound plant extract group are numerically higher than those of the control group.

The above detailed description of the plant compound extract and the preparation method thereof provided by the present invention is provided, and a person skilled in the art may change the concept of the embodiments of the present invention in terms of the specific implementation and the application scope, and in summary, the content of the present description should not be construed as limiting the present invention.

Claims (5)

1. The plant compound extract is characterized by comprising the following components: 2-10 parts of paris polyphylla, 10-15 parts of lophatherum gracile, 10-15 parts of rhus chinensis, 10-15 parts of pteridophyte, 10-15 parts of houttuynia cordata, 18-20 parts of sweet potato and 25-30 parts of dried kelp.

2. The plant compound extract as claimed in claim 1, which is characterized by comprising the following components: 5 parts of paris polyphylla, 10 parts of lophatherum gracile, 15 parts of rhus chinensis, 10 parts of pteridophyte, 10 parts of houttuynia cordata, 20 parts of sweet potato and 30 parts of dried kelp.

3. The plant compound extract as claimed in claim 1, wherein the sweet potato is fresh sweet potato.

4. The preparation method of the plant compound extract is characterized by comprising the following steps:

(1) taking the components of the plant compound extract in the claims 1-3 as raw materials: root of Rhus chinensis Mill, herb of Paris polyphylla Smith, fern, herb of Lophatherum gracile Smith, herba Houttuyniae, sweet potato, and dried herba Zosterae Marinae;

(2) cleaning raw materials, drying in the shade, pulverizing, and mixing;

(3) steaming at 100-;

(4) sterilizing the stock solution, adsorbing with bamboo charcoal for 7-15 days, drying bamboo charcoal in the shade, grinding, separating bamboo charcoal to obtain the final product, and packaging.

5. The preparation method of the plant compound extract as claimed in claim 4, wherein the kelp is a dried kelp extract, and the extraction method comprises:

putting the dried kelp raw material into a closed container, completely freezing at the temperature of minus 30-minus 20 ℃, and repeating for 2-3 times when the kelp raw material is completely thawed at the temperature of 25-30 ℃;

drying the kelp: purified water is prepared according to the weight portion ratio of 1:10, and the pH value is 9: heating in water bath to 40-45 deg.C, maintaining the temperature for 1 hr, and stirring for 10-15 min; heating in water bath, maintaining the temperature, and stirring for 3-8 times;

adding alginate lyase and 2% complex enzyme according to 0.1% of the weight of the dry kelp, stirring for 3-8 hours at the temperature of 40-45 ℃ at the speed of 300-4000 r/min, centrifuging for 10-20 minutes at the speed of 3000-4000r/min, and performing solid-liquid separation to obtain a supernatant which is the kelp extract;

wherein the parameter of the alginate lyase is 1000U/mL;

the compound enzyme is pectinase, cellulase and protease, the ratio of the pectinase to the cellulase to the protease is 1:1:1, and the parameter is 2 ten thousand U/mL.

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