CN113277895B - Composite microbial organic fertilizer and preparation method thereof - Google Patents
Composite microbial organic fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN113277895B CN113277895B CN202110467615.5A CN202110467615A CN113277895B CN 113277895 B CN113277895 B CN 113277895B CN 202110467615 A CN202110467615 A CN 202110467615A CN 113277895 B CN113277895 B CN 113277895B
- Authority
- CN
- China
- Prior art keywords
- organic fertilizer
- compost
- fertilizer
- microbial
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/10—Fertilisers containing plant vitamins or hormones
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/10—Addition or removal of substances other than water or air to or from the material during the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/60—Heating or cooling during the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/80—Separation, elimination or disposal of harmful substances during the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/90—Apparatus therefor
- C05F17/964—Constructional parts, e.g. floors, covers or doors
- C05F17/971—Constructional parts, e.g. floors, covers or doors for feeding or discharging materials to be treated; for feeding or discharging other material
- C05F17/979—Constructional parts, e.g. floors, covers or doors for feeding or discharging materials to be treated; for feeding or discharging other material the other material being gaseous
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/90—Apparatus therefor
- C05F17/964—Constructional parts, e.g. floors, covers or doors
- C05F17/971—Constructional parts, e.g. floors, covers or doors for feeding or discharging materials to be treated; for feeding or discharging other material
- C05F17/986—Constructional parts, e.g. floors, covers or doors for feeding or discharging materials to be treated; for feeding or discharging other material the other material being liquid
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to the technical field of compound microbial organic fertilizers, in particular to a compound microbial organic fertilizer and a preparation method thereof, which comprises the steps of microbial culture, material mixing, compost fermentation, pile turning and liquid supplementing, and airing and storage; because the organic fertilizer is proliferated by harmful microorganisms, the proliferation of the added microbial agent is limited, the time for the compost to enter a fermentation state is difficult to master, and the effect of the compound microbial organic fertilizer is weakened; therefore, the water bath layer is formed at the bottom of the material tray through the expanded perlite arranged in the inorganic fertilizer, the heating effect of the material tray on the bottom surface area of the organic fertilizer is balanced, the water storage performance of the expanded perlite is utilized to intercept moisture evaporated from the bottom in the heating process of the material tray, the water content of the organic fertilizer is kept, the dehydration of the organic fertilizer caused by the heating and temperature rise of the material tray is avoided, the metabolic activity of microorganisms in the organic fertilizer is maintained, and the application effect of the compound microbial organic fertilizer is improved.
Description
Technical Field
The invention relates to the technical field of compound microbial organic fertilizers, in particular to a compound microbial organic fertilizer and a preparation method thereof.
Background
The compound microbial fertilizer is a living microbial product which is formed by compounding specific microbes and nutrient substances, can provide, maintain and improve plant nutrition, improves the yield of agricultural products and the quality of the agricultural products, has the functions of adding microbial strains and inorganic fertilizer components compared with a common microbial organic fertilizer, and can reduce the damage to a soil ecosystem and improve the soil structure.
At present, the compound microbial organic fertilizer used by agricultural planting and farmers is usually applied by adding wastes for planting crops and breeding livestock and cooperating with added microbial agents to carry out composting fermentation to synthesize organic fertilizer, but the organic fertilizer needs longer time to enter the composting state, because the microorganisms carried in the organic fertilizer and the microbial agent added and cultured together consume the nutrients in the organic fertilizer, the proliferation of the microorganisms is in a competitive relationship, meanwhile, the temperature rise caused by the proliferation of microorganisms carried by the organic fertilizer makes the temperature value detected in the composting process difficult to feed back the temperature rise caused by the proliferation of the added microbial agent, so that the proliferation of the microbial agent makes the compost enter a fermentation state, the nutrient content decomposed by the microbial agent in the compost is reduced, the effect of the compound microbial organic fertilizer is weakened, and the application effect on crops is influenced finally.
For example, a Chinese patent with the application number of CN201510316480.7 discloses a compound microbial organic fertilizer and a preparation method thereof, and the compound microbial organic fertilizer is prepared by the following steps: drying radix Puerariae residue to water content of 20-30%, pulverizing to obtain powdered radix Puerariae residue, mixing Lentinus Edodes culture medium waste and powdered radix Puerariae residue uniformly to obtain mixture I, spraying 1-2% ferment bacteria water solution onto the mixture, and standing at 55-65 deg.C for 7-10 days to obtain mixture II; wherein the using amount and the weight ratio of the powdery kudzu root residue, the mushroom culture medium waste and the ferment bacteria are as follows: 200, 300, 400, 600, 1; adding Bacillus megaterium (Bacillus megaterium) thallus into the mixture II, mixing well, performing solid fermentation at 20-30 deg.C for 2-3 days, and turning over once to obtain mixture III; the using amount of the bacillus megaterium is 2-3% of the weight of the mushroom culture medium waste. And air-drying the mixture III to obtain the compound microbial organic fertilizer. The experimental result shows that the technical scheme not only solves the problem of reusing the waste produced in agricultural product processing, but also can greatly improve the cotton yield and reduce the incidence rate of cotton wilt when being used as a cotton fertilizer compared with the prior fertilizer; however, the technical scheme does not solve the problem that in the composting process, the organic fertilizer is proliferated by harmful microorganisms, so that the proliferation of the added microbial agent is limited, and the final application effect of the compound microbial organic fertilizer is influenced.
In view of the above, the invention provides a compound microbial organic fertilizer and a preparation method thereof, and solves the technical problems.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides a compound microbial organic fertilizer and a preparation method thereof.
The compound microbial organic fertilizer comprises 40-90 parts of organic fertilizer, 10-15 parts of inorganic fertilizer and 10-35 parts of microbial agent, wherein the organic fertilizer is loaded into an electric heating tray after being sterilized by flowing steam for 2 hours, and then the microbial agent is added for culture; the organic fertilizer comprises livestock manure, crop straws and vegetable cakes; the livestock manure accounts for 65-75% of the livestock manure, and the rest is the livestock manure; the addition amount of the crop straws is 20-30% of the weight of the livestock manure, and the crop straws are processed into particles in a shape of powder; the vegetable cake is mixed into the organic fertilizer after being soaked in water for 5-7 min; the inorganic fertilizer also comprises expanded perlite, the particle size of the expanded perlite is 4-7mm, and the expanded perlite is stacked at the bottom of the material tray to have the thickness of 24-32 mm; the microbial agent comprises bacillus, mould and cellulose; the bacillus and the mould are directly added into the organic fertilizer in the material tray and participate in the fermentation of the organic fertilizer; the cellulose bacteria are finally added into a microbial organic fertilizer;
in the prior art, microorganisms carried by organic fertilizers and microbial agents added and cultured in the organic fertilizers consume nutrients in the organic fertilizers together, so that the proliferation among the microorganisms is in a competitive relationship, and meanwhile, the temperature rise formed by the proliferation of the microorganisms carried by the organic fertilizers makes the temperature value detected in the composting process difficult to feed back the temperature rise formed by the proliferation of the added microbial agents, so that the proliferation of the microbial agents makes the compost enter a fermentation state, the nutrient content decomposed by the microbial agents in the compost is reduced, the effect of the compound microbial organic fertilizer is weakened, and the application effect on crops is influenced finally;
therefore, the invention loads the organic fertilizer into the electric heating material tray, controls the environmental temperature of the microbial agent by heating the material tray, promotes the temperature of the organic fertilizer to rise to the temperature range suitable for the proliferation of bacillus and mould fungi, and simultaneously plays a role in inactivating the normal-temperature breeding strains in the livestock manure, so that the added microbial agent can be conveniently and rapidly proliferated, the organic fertilizer enters the compost fermentation state, a water bath layer is formed at the bottom of the material tray by pre-filling the water at the bottom of the material tray and matching with the expanded perlite arranged in the inorganic fertilizer, the heating effect of the material tray is balanced in the bottom area of the organic fertilizer, the condition that the normal-temperature breeding strains in the organic fertilizer are not completely inactivated is avoided, the proliferation of the added microbial agent is ensured, the organic fertilizer enters the fermentation state, and the water storage performance of the expanded perlite is also utilized, the water content evaporated from the bottom in the heating process of the material tray is intercepted, the water content of the organic fertilizer is kept, the dehydration of the organic fertilizer caused by the heating and temperature rise of the material tray is avoided, the metabolic activity of microorganisms in the organic fertilizer is maintained, and therefore the application effect of the compound microbial organic fertilizer is improved.
The invention relates to a preparation method of a composite microbial organic fertilizer, which is suitable for the composite microbial organic fertilizer and comprises the following steps:
s1, microbial culture: adding 10 parts by weight of organic fertilizer into a tray, wherein tap water with the depth of 1-2cm is filled in the tray in advance, adding 2 parts by weight of microbial inoculum into the tray after the tray is irradiated by sunlight for 2 hours, then adding 0.04 parts by weight of vitamin B and vitamin C particles into the tray every 12 hours, and continuously fermenting for 72 hours;
s2, mixing materials: in the process of adding the organic fertilizer in S1, the livestock manure is layered through the crushed crop straws, then the vegetable cake is thrown in each layer of the layered livestock manure, the quantity of the thrown vegetable cake is controlled to be gradually increased from the bottom layer of the livestock manure, and the height of the fertilizer accumulated in the material tray is maintained at 0.6-0.9 m;
s3, composting and fermenting: after the fertilizer in S2 is piled up, ventilating holes on the compost every 8-12h, measuring the temperature of the holes of the compost by using a thermometer, and covering the surface of the compost with a sunshade net and a plastic film when continuous illumination and raining occur in the weather in the process to keep the compost in the range of the temperature and humidity state;
s4, pile-turning and liquid supplementing: turning the compost in S3 after 5-8 days of fermentation and temperature decrease in the jacks, supplementing nutrient solution of microbial agent, performing the process once a day for 2-5 days, and finally adding inorganic fertilizer;
s5, airing and storing: and (4) turning the compost in the S4, standing the compost for two to three weeks to achieve a rotten state, opening the compost in a sunny day to evaporate water to be reduced to below 25%, mixing the compost with the inorganic fertilizer, and storing the compost in a cool place.
Preferably, in the microbial cultivation process, the temperature of the organic fertilizer is controlled to be 40-75 ℃ by heating the material tray, and the heating state of the material tray intermittently runs in a cycle of 20-40 min: in the culture process of the microbial inoculum in the organic fertilizer, the material disc is circularly heated, so that the proliferation process of the microbial inoculum in the organic fertilizer is in an intermittent high-activity state, the proliferation speed of microorganisms is further controlled, the nutrient in the organic fertilizer is prevented from being exhausted after the microorganisms are exponentially proliferated, the microorganism in the organic fertilizer is prevented from being inactivated in the subsequent untimely pile-turning liquid supplementing process, the proliferation state of the microbial inoculum in the organic fertilizer is maintained, the composting fermentation process of the organic fertilizer is further controlled, and the preparation effect of the compound microbial organic fertilizer is improved.
Preferably, in the compost fermentation process, after the hole inserting operation is carried out, a water absorbing paper strip is inserted into the hole inserting operation, the water absorbing paper strip is wound into a cylindrical shape, and the top of the water absorbing paper strip protrudes out of the surface of the compost; in the process of composting fermentation, water stored in the expanded perlite at the bottom of the material tray can be conducted out along gaps among the organic fertilizers along with the gradually increased temperature and the reduced water content of the organic fertilizers in the fermentation state, the water content of the organic fertilizers is maintained, the water absorbing strips extending into the composting insertion holes are utilized, the capillary effect of the contact water between the bottom of the water absorbing strips and the expanded perlite is utilized, the water content in the compost is reflected, when the water absorbing strips protrude out of the surface of the compost and do not appear spread water marks, water is supplemented through the edge part of the material tray, the supplemented water amount is controlled not to overflow the height of the expanded perlite, the water required in the composting process of the organic fertilizers is maintained, and the preparation effect of the composite microbial organic fertilizers is maintained.
Preferably, the middle part of the water absorbing paper strip is also provided with a cutting seam, the water absorbing paper piece unfolded at the cutting seam faces to the outside organic fertilizer, and a hollow structure is formed on the cylinder surface of the water absorbing paper strip; the actual condition of organic fertilizer water content among the compost fermentation process, through the joint-cutting of seting up on the paper slip face of absorbing water, the regional contact of the paper slip that absorbs water that makes joint-cutting department expand with organic fertilizer, then take out the paper slip that absorbs water in the jack, observe the water content that the water mark state of joint-cutting department obtained organic fertilizer, when the regional water content of organic fertilizer is lower, utilize the capillary effect of moisture transmission on the paper slip that absorbs water, conduct the water conduction of charging tray bottom expanded perlite to the organic fertilizer of joint-cutting department in, the water content state of organic fertilizer department is maintained, thereby compound microbial organic fertilizer's preparation effect has been stabilized.
Preferably, the temperature change of each reaction stage in the compost fermentation process comprises the following steps:
i. a heating stage: in the initial stage of the organic fertilizer accumulation on the material tray, the metabolic activity of microorganisms mainly comprises added spores and molds, and the compost enters a fermentation state under the heating action of the material tray, organic substances are rapidly proliferated and decomposed under aerobic conditions, and the temperature of the compost is raised to 40-50 ℃ by heat generation;
ii. And (3) high-temperature stage: along with the increased temperature in the step i, the organic fertilizer replaces the dominant action of mesophilic microorganisms in the organic fertilizer, the temperature of the compost is increased to 55-65 ℃ within the following 2-5 days, the temperature is increased, the pectin substances in the organic fertilizer are decomposed, the heat of the compost is continuously increased to 70-80 ℃, the thermophilic microorganisms in the organic fertilizer are also in a death state, and the compost in a fermentation state is in a decomposition state;
iii, cooling stage: when the compost in the decomposed state in the step ii lasts for a period of time, the remaining lignin which is not decomposed becomes humus, the activity of microorganisms is weakened due to the depletion of the decomposition of organic nutrients, and the process of turning and replenishing liquid is directly carried out after the temperature of the compost is detected to be reduced to below 38 ℃ within 5 days;
iv, decomposing and fertilizer preserving stage: after the compost height is observed to be reduced by 20%, the compost is compacted to be in an anaerobic state, and the effect of decomposing organic fertilizers by microorganisms is reduced, so that the fertilizer preservation effect is achieved.
The invention has the following beneficial effects:
1. according to the invention, the expanded perlite arranged in the inorganic fertilizer is used for forming the water bath layer at the bottom of the material tray, so that the heating effect of the material tray on the bottom surface area of the organic fertilizer is balanced, and the water storage performance of the expanded perlite is utilized for intercepting moisture evaporated from the bottom in the heating process of the material tray, so that the water content of the organic fertilizer is maintained, the dehydration of the organic fertilizer caused by the heating temperature rise of the material tray is avoided, and the metabolic activity of microorganisms in the organic fertilizer is maintained.
2. According to the invention, the material tray is heated circularly, so that the proliferation process of the microbial inoculum in the organic fertilizer is in an intermittent high-activity state, the proliferation speed of the microorganisms is further controlled, the microorganisms are prevented from being exhausted after exponential proliferation, the microorganisms in the organic fertilizer are prevented from being inactivated in the subsequent untimely pile-turning liquid supplementing process, the proliferation state of the microbial inoculum in the organic fertilizer is maintained, and the composting fermentation process of the organic fertilizer is further controlled.
3. According to the invention, the water content in the compost is reflected by the capillary effect of the contact moisture of the bottom of the water absorbing paper strips extending into the compost insertion holes and the expanded perlite, when the water absorbing paper strips protrude out of the surface of the compost and no spreading water stain appears, the water is supplemented through the edge part of the charging tray, the height of the expanded perlite is controlled, and the moisture required by the organic fertilizer composting process is maintained.
Drawings
The invention is further described below with reference to the drawings and the embodiments.
FIG. 1 is a flow chart of a method for preparing a compound microbial organic fertilizer in the invention;
FIG. 2 is a flow chart showing the temperature change in each reaction stage in the compost fermentation process of the present invention;
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in figures 1-2, the compound microbial organic fertilizer comprises 40-90 parts of organic fertilizer, 10-15 parts of inorganic fertilizer and 10-35 parts of microbial agent, wherein the organic fertilizer is loaded into an electric heating tray after being sterilized by flowing steam for 2 hours, and then the microbial agent is added for culture; the organic fertilizer comprises livestock manure, crop straws and vegetable cakes; the livestock manure accounts for 65-75% of the livestock manure, and the rest is the livestock manure; the addition amount of the crop straws is 20-30% of the weight of the livestock manure, and the crop straws are processed into particles in a shape of powder; the vegetable cake is mixed into the organic fertilizer after being soaked in water for 5-7 min; the inorganic fertilizer also comprises expanded perlite, the particle size of the expanded perlite is 4-7mm, and the expanded perlite is stacked at the bottom of the material tray to have the thickness of 24-32 mm; the microbial agent comprises bacillus, mould and cellulose; the bacillus and the mould are directly added into the organic fertilizer in the material tray and participate in the fermentation of the organic fertilizer; the cellulose bacteria are finally added into a microbial organic fertilizer;
in the prior art, microorganisms carried by organic fertilizers and microbial agents added and cultured in the organic fertilizers consume nutrients in the organic fertilizers together, so that the proliferation among the microorganisms is in a competitive relationship, and meanwhile, the temperature rise formed by the proliferation of the microorganisms carried by the organic fertilizers makes the temperature value detected in the composting process difficult to feed back the temperature rise formed by the proliferation of the added microbial agents, so that the proliferation of the microbial agents makes the compost enter a fermentation state, the nutrient content decomposed by the microbial agents in the compost is reduced, the effect of the compound microbial organic fertilizer is weakened, and the application effect on crops is influenced finally;
therefore, the organic fertilizer is loaded into the electric heating material tray, the environment temperature of the microbial agent is controlled by heating the material tray, the temperature of the organic fertilizer is promoted to rise to the temperature range suitable for the proliferation of bacillus and mould, and simultaneously, the microbial agent in the livestock manure is inactivated at normal temperature, so that the added microbial agent is rapidly proliferated, the organic fertilizer enters a compost fermentation state, a water bath layer is formed at the bottom of the material tray by pre-filling water at the bottom of the material tray and matching with expanded perlite arranged in the inorganic fertilizer, the heating effect of the material tray on the bottom area of the organic fertilizer is balanced, the condition that the normal-temperature propagating strains in the organic fertilizer are not completely inactivated is avoided, the proliferation of the added microbial agent is ensured, the organic fertilizer enters the fermentation state, and the water storage performance of the expanded perlite is also utilized, the water content evaporated from the bottom in the heating process of the material tray is intercepted, the water content of the organic fertilizer is kept, the dehydration of the organic fertilizer caused by the heating and temperature rise of the material tray is avoided, the metabolic activity of microorganisms in the organic fertilizer is maintained, and therefore the application effect of the compound microbial organic fertilizer is improved.
The invention relates to a preparation method of a composite microbial organic fertilizer, which is suitable for the composite microbial organic fertilizer and comprises the following steps:
s1, microbial culture: adding 10 parts by weight of organic fertilizer into a tray, wherein tap water with the depth of 1-2cm is filled in the tray in advance, adding 2 parts by weight of microbial inoculum into the tray after the tray is irradiated by sunlight for 2 hours, then adding 0.04 parts by weight of vitamin B and vitamin C particles into the tray every 12 hours, and continuously fermenting for 72 hours;
s2, mixing materials: in the organic fertilizer adding process of S1, stacking the livestock manure layer by layer through the crushed crop straws, then throwing the paraquat in each layer of the stacked livestock manure, controlling the amount of the thrown paraquat to gradually increase from the bottom layer of the livestock manure, and maintaining the height of the fertilizer stacked in the material tray at 0.6-0.9 m;
s3, composting and fermenting: after the fertilizer in S2 is piled up, ventilating holes on the compost every 8-12h, measuring the temperature of the holes of the compost by using a thermometer, and covering the surface of the compost with a sunshade net and a plastic film when continuous illumination and raining occur in the weather in the process to keep the compost in the range of the temperature and humidity state;
s4, pile-turning and liquid supplementing: turning over the compost in S3 after fermenting for 5-8 days and the temperature detected in the jack is reduced, supplementing nutrient solution of microbial agent, and adding inorganic fertilizer once a day for 2-5 days;
s5, airing and storing: and (4) turning the compost in the S4, standing the compost for two to three weeks to achieve a thoroughly decomposed state, opening the compost in a sunny day to reduce the water evaporation to below 25%, mixing the compost with an inorganic fertilizer, and storing the compost in a cool place.
In one embodiment of the present invention, in the microorganism culturing step, the temperature of the organic fertilizer is controlled to 40 to 75 ℃ by heating the tray, and the heating state of the tray is intermittently operated for 20 to 40 min: in the culture process of the microbial inoculum in the organic fertilizer, the material disc is circularly heated, so that the proliferation process of the microbial inoculum in the organic fertilizer is in an intermittent high-activity state, the proliferation speed of microorganisms is further controlled, the nutrient in the organic fertilizer is prevented from being exhausted after the microorganisms are exponentially proliferated, the microorganism in the organic fertilizer is prevented from being inactivated in the subsequent untimely pile-turning liquid supplementing process, the proliferation state of the microbial inoculum in the organic fertilizer is maintained, the composting fermentation process of the organic fertilizer is further controlled, and the preparation effect of the compound microbial organic fertilizer is improved.
In the compost fermentation process, after the hole inserting operation is carried out, a water absorbing paper strip is inserted into the compost fermentation process, the water absorbing paper strip is wound into a cylindrical shape, and the top of the water absorbing paper strip protrudes out of the surface of the compost; in the process of composting fermentation, water stored in the expanded perlite at the bottom of the material tray can be conducted out along gaps among the organic fertilizers along with the gradually increased temperature and the reduced water content of the organic fertilizers in the fermentation state, the water content of the organic fertilizers is maintained, the water absorbing strips extending into the composting insertion holes are utilized, the capillary effect of the contact water between the bottom of the water absorbing strips and the expanded perlite is utilized, the water content in the compost is reflected, when the water absorbing strips protrude out of the surface of the compost and do not appear spread water marks, water is supplemented through the edge part of the material tray, the supplemented water amount is controlled not to overflow the height of the expanded perlite, the water required in the composting process of the organic fertilizers is maintained, and the preparation effect of the composite microbial organic fertilizers is maintained.
As an embodiment of the invention, the middle part of the water absorbing paper strip is also provided with a cutting seam, the water absorbing paper sheet expanded at the cutting seam faces to the organic fertilizer outside, and a hollow structure is formed on the cylinder surface of the water absorbing paper strip; the actual condition of organic fertilizer water content among the compost fermentation process, through the joint-cutting of seting up on the paper slip face of absorbing water, the regional contact of the paper slip that absorbs water that makes joint-cutting department expand with organic fertilizer, then take out the paper slip that absorbs water in the jack, observe the water content that the water mark state of joint-cutting department obtained organic fertilizer, when the regional water content of organic fertilizer is lower, utilize the capillary effect of moisture transmission on the paper slip that absorbs water, conduct the water conduction of charging tray bottom expanded perlite to the organic fertilizer of joint-cutting department in, the water content state of organic fertilizer department is maintained, thereby compound microbial organic fertilizer's preparation effect has been stabilized.
In one embodiment of the present invention, the temperature change in each reaction stage in the compost fermentation process comprises the following steps:
i. a heating stage: in the initial stage of the organic fertilizer accumulation on the material tray, the metabolic activity of microorganisms mainly comprises added spores and molds, and the compost enters a fermentation state under the heating action of the material tray, organic substances are rapidly proliferated and decomposed under aerobic conditions, and the temperature of the compost is raised to 40-50 ℃ by heat generation;
ii. And (3) high-temperature stage: along with the increased temperature in the step i, the organic fertilizer replaces the dominant action of mesophilic microorganisms in the organic fertilizer, the temperature of the compost is increased to 55-65 ℃ within the following 2-5 days, the temperature is increased, the pectin substances in the organic fertilizer are decomposed, the heat of the compost is continuously increased to 70-80 ℃, the thermophilic microorganisms in the organic fertilizer are also in a death state, and the compost in a fermentation state is in a decomposition state;
iii, cooling stage: after the compost in the decomposed state in the step ii lasts for a period of time, remaining lignin which is not decomposed into humus, weakening the activity of microorganisms due to the depletion of the decomposition of organic nutrients, and directly performing a process of turning and replenishing liquid after detecting that the temperature of the compost is reduced to below 38 ℃ within 5 days;
iv, decomposing and fertilizer preserving stage: after the compost height is observed to be reduced by 20%, the compost is compacted to be in an anaerobic state, and the effect of decomposing organic fertilizers by microorganisms is reduced, so that the fertilizer preservation effect is achieved.
The specific working process is as follows:
the organic fertilizer is loaded into the electric heating material tray, the environmental temperature of the microbial agent is controlled by heating the material tray, the temperature of the organic fertilizer is promoted to rise to the temperature range suitable for proliferation of bacillus and mould, meanwhile, the inactivation effect is realized on normal-temperature breeding strains in the livestock manure, the added microbial agent is convenient to rapidly proliferate, the organic fertilizer enters a compost fermentation state, a water bath layer is formed at the bottom of the material tray through the water filled in the bottom of the material tray in advance and matched with expanded perlite arranged in the inorganic fertilizer, the heating effect of the material tray is balanced in the bottom area of the organic fertilizer, the condition that the normal-temperature breeding strains in the organic fertilizer are not completely inactivated is avoided, the proliferation of the added microbial agent is ensured, the organic fertilizer in the compost enters the fermentation state, the water storage performance of the expanded perlite is also utilized, the moisture evaporated from the bottom in the heating process of the material tray is separated, the water content of the organic fertilizer is kept, the dehydration of the organic fertilizer caused by the heating temperature rise of the material tray is avoided, and the metabolic activity of microorganisms in the organic fertilizer is maintained; the material tray is heated circularly, so that the proliferation process of the microbial inoculum in the organic fertilizer is in an intermittent high-activity state, the proliferation speed of the microorganisms is controlled, the microorganisms are prevented from being exponentially proliferated and exhausted, the microorganisms in the organic fertilizer are prevented from being inactivated in the subsequent untimely pile-turning liquid supplementing process, the proliferation state of the microbial inoculum in the organic fertilizer is maintained, and the composting fermentation process of the organic fertilizer is controlled; the water content in the compost is reflected by the capillary effect of the contact moisture of the bottom of the water absorbing paper strips extending into the compost jacks and the expanded perlite, when the water absorbing paper strips protrude out of the surface of the compost and no spreading water stain appears, water is supplemented through the edge part of the material tray, the supplemented water quantity is controlled not to exceed the height of the expanded perlite, and the moisture required in the organic fertilizer composting process is maintained; through the joint-cutting of seting up on the paper slip face of absorbing water, make the regional contact of the paper slip of absorbing water that the joint-cutting department expanded with organic fertilizer, then take out the paper slip of absorbing water in the jack, observe the water mark state of joint-cutting department and obtain the water content of organic fertilizer, when the water content in organic fertilizer region is lower, utilize the capillary effect of moisture transmission on the paper slip of absorbing water, in the organic fertilizer of water transfer to joint-cutting department, maintain the water content state of organic fertilizer department.
To verify the practical application effect of the present invention, the following experiments were performed:
providing a composite microbial organic fertilizer preparation method for farmers, respectively detecting various parameters of compost in the composite microbial organic fertilizer preparation method by adopting the composite microbial organic fertilizer preparation method, the composite microbial organic fertilizer preparation method without adding expanded perlite, the composite microbial organic fertilizer preparation method without adopting absorbent paper strips and the composite microbial organic fertilizer preparation method adopted by the farmers in the past, and recording data of the parameters are as shown in the following table;
experiment group 1, the compound microbial organic fertilizer of the invention is used:
experiment group 2, the compound microbial organic fertilizer of the invention is used and expanded perlite is not added:
experiment group 3, the composite microbial organic fertilizer of the invention is used and the detection is carried out without adopting absorbent paper strips:
experiment group 4, compound microbial organic fertilizer used by farmers in the past:
according to the experimental group, during the production process of different preparation methods of the compound microbial organic fertilizer, the composting parameter performance has different detection data;
in the first experimental group, by using the preparation method of the compound microbial organic fertilizer, the data of the compound microbial organic fertilizer performance is best, and the retention capacity of the organic matter content of the compost is best;
in the second experimental group, the preparation method of the compound microbial organic fertilizer is used, expanded perlite is not added, the water content in the early stage of composting is low, the temperature of compost fermentation and storage is high, the activity of ions in the compost is reduced due to the low water content, the conductivity is reduced, the metabolic activity of microbes is reduced, the organic matter content in the early stage composting is high, and after water is supplemented in the middle stage of composting, the microbes are rapidly proliferated to accumulate and consume the organic matter content, so that the final organic matter content is low;
in the third experimental group, the composite microbial organic fertilizer preparation method is used, water absorbing paper strips are not adopted for detection, the water content in compost is low in the later period of the compost due to the fact that the water absorbing paper strips are not used for detection, the temperature for decomposing and accumulating the compost is high, the activity of ions in the compost is reduced due to low water, the final conductivity is reduced, and in the early period of the compost, under the state of high water content, a large amount of organic matters are consumed due to rapid proliferation of microorganisms, and under the state of insufficient water in the subsequent period, extra organic matters are also consumed in the slow death process of a large amount of microorganisms, so that the final organic matter content is low;
in the experiment group IV, the detection parameters of the compost detected by the method for preparing the compound microbial organic fertilizer adopted by farmers in the past are similar to those of the experiment group I, and although the conductivity performance is better, the content of organic matters in the compost is lower, so that the application effect of the compound microbial organic fertilizer is influenced;
in conclusion, the preparation method of the compound microbial organic fertilizer disclosed by the invention can be found out, the organic matter content in the compound microbial organic fertilizer is improved, and the time for composting to enter a fermentation process is also reduced, so that the use effect of the preparation method of the compound microbial organic fertilizer is improved.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (3)
1. A preparation method of a compound microbial organic fertilizer is characterized by comprising the following steps: the fertilizer comprises 40-90 parts of organic fertilizer, 10-15 parts of inorganic fertilizer and 10-35 parts of microbial agent, and is characterized in that: after the organic fertilizer is sterilized by flowing steam for 2 hours, loading the organic fertilizer into an electric heating tray, and then adding a microbial agent for culture; the organic fertilizer comprises livestock manure, crop straws and vegetable cakes; the livestock manure accounts for 65-75% of the livestock manure, and the rest is the livestock manure; the addition amount of the crop straws is 20-30% of the weight of the livestock manure, and the crop straws are processed into particles in a shape of powder; the vegetable cake is mixed into the organic fertilizer after being soaked in water for 5-7 min; the inorganic fertilizer also comprises expanded perlite, the particle size of the expanded perlite is 4-7mm, and the expanded perlite is stacked at the bottom of the material tray to have the thickness of 24-32 mm; the microbial agent comprises bacillus, mould and cellulose; the bacillus and the mould are directly added into the organic fertilizer in the material tray and participate in the fermentation of the organic fertilizer; the cellulose bacteria are finally added into the microbial organic fertilizer, and the preparation method comprises the following steps:
s1, microbial culture: adding 10 parts by weight of organic fertilizer into a tray, wherein tap water with the depth of 1-2cm is filled in the tray in advance, adding 2 parts by weight of microbial inoculum into the tray after the tray is irradiated by sunlight for 2 hours, then adding 0.04 parts by weight of vitamin B and vitamin C particles into the tray every 12 hours, and continuously fermenting for 72 hours;
s2, mixing materials: in the process of adding the organic fertilizer in S1, the livestock manure is layered through the crushed crop straws, then the vegetable cake is thrown in each layer of the layered livestock manure, the quantity of the thrown vegetable cake is controlled to be gradually increased from the bottom layer of the livestock manure, and the height of the fertilizer accumulated in the material tray is maintained at 0.6-0.9 m;
s3, composting and fermenting: after the fertilizer in S2 is piled up, ventilating holes on the compost every 8-12h, measuring the temperature of the holes of the compost by using a thermometer, and covering the surface of the compost with a sunshade net and a plastic film when continuous illumination and raining occur in the weather in the process to keep the compost in the range of the temperature and humidity state;
s4, pile-turning and liquid supplementing: turning over the compost in S3 after fermenting for 5-8 days and the temperature detected in the jack is reduced, supplementing nutrient solution of microbial agent, and adding inorganic fertilizer once a day for 2-5 days;
s5, airing and storing: turning the compost in S4, standing the compost for two to three weeks to achieve a rotten state, opening the compost in a sunny day to evaporate water to reduce the water content to below 25%, mixing the compost with an inorganic fertilizer, and storing the compost in a cool place;
in the compost fermentation procedure, after the operation of inserting holes is carried out, water absorbing paper strips are inserted into the holes, the water absorbing paper strips are wound into a cylindrical shape, and the tops of the water absorbing paper strips protrude out of the surface of the compost;
and the middle part of the water absorbing paper strip is also provided with a cutting seam, the water absorbing paper piece unfolded at the cutting seam faces to the outside organic fertilizer, and a hollow structure is formed on the cylinder surface of the water absorbing paper strip.
2. The preparation method of the compound microbial organic fertilizer as claimed in claim 1, wherein the preparation method comprises the following steps: in the microbial culture process, the temperature of the organic fertilizer is controlled to be 40-75 ℃ by heating the material tray, and the heating state of the material tray is operated intermittently in a period of 20-40 min.
3. The preparation method of the compound microbial organic fertilizer as claimed in claim 1, wherein the preparation method comprises the following steps: the temperature change of each reaction stage in the compost fermentation process comprises the following steps:
i. a heating stage: in the initial stage of the organic fertilizer accumulation on the material tray, the metabolic activity of microorganisms mainly comprises added spores and molds, and the compost enters a fermentation state under the heating action of the material tray, organic substances are rapidly proliferated and decomposed under aerobic conditions, and the temperature of the compost is raised to 40-50 ℃ by heat generation;
ii. And (3) high-temperature stage: along with the increased temperature in the step i, the organic fertilizer replaces the dominant action of mesophilic microorganisms in the organic fertilizer, the temperature of the compost is increased to 55-65 ℃ within the following 2-5 days, the temperature is increased, the pectin substances in the organic fertilizer are decomposed, the heat of the compost is continuously increased to 70-80 ℃, the thermophilic microorganisms in the organic fertilizer are also in a death state, and the compost in a fermentation state is in a decomposition state;
iii, cooling stage: when the compost in the decomposed state in the step ii lasts for a period of time, the remaining lignin which is not decomposed becomes humus, the activity of microorganisms is weakened due to the depletion of the decomposition of organic nutrients, and the process of turning and replenishing liquid is directly carried out after the temperature of the compost is detected to be reduced to below 38 ℃ within 5 days;
iv, decomposing and fertilizer preserving stage: after the compost height is observed to be reduced by 20%, the compost is compacted to be in an anaerobic state, and the effect of decomposing organic fertilizers by microorganisms is reduced, so that the fertilizer preservation effect is achieved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110467615.5A CN113277895B (en) | 2021-04-28 | 2021-04-28 | Composite microbial organic fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110467615.5A CN113277895B (en) | 2021-04-28 | 2021-04-28 | Composite microbial organic fertilizer and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113277895A CN113277895A (en) | 2021-08-20 |
| CN113277895B true CN113277895B (en) | 2022-05-06 |
Family
ID=77277588
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110467615.5A Active CN113277895B (en) | 2021-04-28 | 2021-04-28 | Composite microbial organic fertilizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113277895B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928160A (en) * | 2010-06-24 | 2010-12-29 | 安徽省农业科学院土壤肥料研究所 | Method for composting straws in compost frame under high temperature |
| CN103524182A (en) * | 2013-10-23 | 2014-01-22 | 青岛嘉禾丰肥业有限公司 | Method for preparing fertilizer for promoting root system growth |
| CN105294339A (en) * | 2015-11-06 | 2016-02-03 | 遵义金素贸易有限公司道真鸿梁分公司 | Slow release fertilizer used for Chinese prickly ash |
| CN106008071A (en) * | 2016-05-16 | 2016-10-12 | 林志仓 | Fertilizer with expanded perlite as carrier and preparation method and application thereof |
| CN106083313A (en) * | 2016-06-08 | 2016-11-09 | 杨子正 | A kind of composite microbiological fertilizer and preparation method thereof |
| CN107162830A (en) * | 2017-07-28 | 2017-09-15 | 眉山市金威特化肥有限公司 | A kind of humic absolute acid stability compound fertilizer and preparation method thereof |
| CN107445727A (en) * | 2017-09-22 | 2017-12-08 | 安徽亚泰天然植物科技有限公司 | A kind of plantation fertilizer for improving peanut grease content and preparation method |
| CN111559927A (en) * | 2020-05-08 | 2020-08-21 | 江南大学 | Method for preparing organic fertilizer by fermenting pig manure |
| CN111777452A (en) * | 2020-06-28 | 2020-10-16 | 中国农业大学 | Sheep manure composting method |
| CN112794763A (en) * | 2021-01-14 | 2021-05-14 | 唐世强 | Method for preparing bio-organic fertilizer by using corn steep liquor concentrated solution |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101702995B (en) * | 2009-11-20 | 2011-01-05 | 宁夏农林科学院 | Method for producing vegetable cultivation medium and seedling culturing medium by using caragana microphylla |
| CN111039703A (en) * | 2019-12-30 | 2020-04-21 | 北京市新海园林工程有限公司 | Garden waste composting process and composting product prepared by same |
-
2021
- 2021-04-28 CN CN202110467615.5A patent/CN113277895B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928160A (en) * | 2010-06-24 | 2010-12-29 | 安徽省农业科学院土壤肥料研究所 | Method for composting straws in compost frame under high temperature |
| CN103524182A (en) * | 2013-10-23 | 2014-01-22 | 青岛嘉禾丰肥业有限公司 | Method for preparing fertilizer for promoting root system growth |
| CN105294339A (en) * | 2015-11-06 | 2016-02-03 | 遵义金素贸易有限公司道真鸿梁分公司 | Slow release fertilizer used for Chinese prickly ash |
| CN106008071A (en) * | 2016-05-16 | 2016-10-12 | 林志仓 | Fertilizer with expanded perlite as carrier and preparation method and application thereof |
| CN106083313A (en) * | 2016-06-08 | 2016-11-09 | 杨子正 | A kind of composite microbiological fertilizer and preparation method thereof |
| CN107162830A (en) * | 2017-07-28 | 2017-09-15 | 眉山市金威特化肥有限公司 | A kind of humic absolute acid stability compound fertilizer and preparation method thereof |
| CN107445727A (en) * | 2017-09-22 | 2017-12-08 | 安徽亚泰天然植物科技有限公司 | A kind of plantation fertilizer for improving peanut grease content and preparation method |
| CN111559927A (en) * | 2020-05-08 | 2020-08-21 | 江南大学 | Method for preparing organic fertilizer by fermenting pig manure |
| CN111777452A (en) * | 2020-06-28 | 2020-10-16 | 中国农业大学 | Sheep manure composting method |
| CN112794763A (en) * | 2021-01-14 | 2021-05-14 | 唐世强 | Method for preparing bio-organic fertilizer by using corn steep liquor concentrated solution |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113277895A (en) | 2021-08-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Meena et al. | Composting: phases and factors responsible for efficient and improved composting | |
| CN102173928B (en) | Wood non-woven fabric container nursery biological-type medium | |
| CN104725085A (en) | Solanaceous vegetable seedling medium prepared by taking vegetable waste compost as fertilizer source and preparation method of solanaceous vegetable seedling medium | |
| CN109437989B (en) | Method for preparing culture medium by using pecan shells as raw materials | |
| CN104844382B (en) | A kind of organic composite fertilizer of suitable asparagus lettuce growth | |
| CN102409013B (en) | Organic material inoculant and preparation method thereof | |
| CN104788244A (en) | Organic fertilizer and preparation method thereof | |
| CN105948846A (en) | Organic fertilizer special for potted flowers | |
| CN103172421A (en) | Treatment method of fruit/vegetable waste | |
| CN106278482A (en) | A kind of potato primary stock seedling medium and preparation method | |
| CN105316249A (en) | Bacillus subtilis, microbial agent and application of bacillus subtilis and microbial agent to fermentation compost maturity | |
| CN104177138B (en) | Biological bacterial fertilizer prepared through solid fermentation technology and application of biological bacterial fertilizer | |
| CN113508739A (en) | Organic nutritional type culture medium and preparation method thereof | |
| CN104671859A (en) | Method for producing mushroom dreg fertilizer by using medium wastes after cultivating edible mushrooms with crop straws | |
| CN109055262A (en) | A kind of complex micro organism fungicide and preparation method thereof for feces of livestock and poultry low temperature compost fermentation | |
| CN106258975A (en) | A kind of potato primary stock production method | |
| EP2828225B1 (en) | Method for composting spent mushroom compost | |
| CN107867950A (en) | A kind of biological organic fertilizer prepared by waste edible fungus bacteria stick | |
| CN112166731B (en) | Method for improving physical and chemical properties of soil | |
| CN103951479B (en) | Fertilizer and preparation method thereof | |
| CN113277895B (en) | Composite microbial organic fertilizer and preparation method thereof | |
| CN109879682B (en) | Method for deep fermentation of organic fertilizer by internal ventilation, heat preservation and moisture preservation | |
| CN106171517A (en) | The cultivation matrix of a kind of Dictyophora echino-volvata Zane and cultural method thereof | |
| CN110903120A (en) | Production method of organic-inorganic compound fertilizer taking plant straws as raw materials | |
| CN106083295A (en) | A kind of organic substrate utilized altogether based on Fructus Lycopersici esculenti straw and vinegar and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220419 Address after: 150000 No. 1, printing and dyeing street, Xiangfang District, Harbin, Heilongjiang Province Applicant after: HARBIN OASIS STAR BIOTECHNOLOGY Co.,Ltd. Address before: 239400 Room 501, building 2, xiangzhangyuan community, No. 599 Zhaoxin Road, Mingguang City, Chuzhou City, Anhui Province Applicant before: Wei Huaqiang |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |