CN113265400A - 一种合成Headful包装型噬菌体的方法 - Google Patents
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Abstract
本发明提供用于合成headful包装型噬菌体的多核苷酸。本发明还提供包含所述多核苷酸的载体和细胞，以及使用本发明的多核苷酸或载体合成Headful包装型噬菌体的方法。
Description
技术领域
本发明涉及生物工程领域。具体而言，本发明涉及一种合成Headful包装型噬菌体的方法。
背景技术
噬菌体是指能够感染细菌并在其宿主体内复制和增殖的病毒。噬菌体对宿主具有极强的专一性，因此被广泛用于抗菌治疗。尤其是随着抗生素的滥用导致耐药病原菌的出现，应用噬菌体治疗耐药病原菌引起越来越多的关注。但是，病原菌会对天然噬菌体产生抗性，并且天然噬菌体可能编码毒力因子或毒素。因此，对天然噬菌体进行修饰有助于获得具备理想性能的合成噬菌体。
噬菌体合成包括两个步骤，分别是噬菌体基因组的组装和合成基因组的重启。在目前报道的噬菌体合成工作中基因组的组装技术主要包括Gibson Assembly和酵母转化相关的重组(TAR)克隆。
Gibson Assembly可以在体外将几个(如3-6个)DNA片段组装成环状的噬菌体基因组。但用Gibson Assembly组装噬菌体DNA，当噬菌体基因组较大(如超过20kb)时，则需要使用细菌宿主进行噬菌体DNA片段的克隆。而很多含有噬菌体遗传元件的DNA 片段编码对细菌宿主有毒的产物，不能用大肠杆菌(E.coli)等克隆宿主进行稳定克隆和增殖，限制了Gibson Assembly在噬菌体合成中的应用。
噬菌体的毒力基因在酵母中不表达，因此以酵母为宿主对噬菌体DNA片段进行克隆，可以克服噬菌体DNA对细菌宿主毒性的问题。酵母转化相关的重组(TAR)克隆是利用酵母细胞强大的同源重组能力将多个具有同源末端的噬菌体基因组片段高效地组装到酵母人工染色体(YAC)上，得到含有噬菌体基因组的重组质粒。
噬菌体基因组的重启是指从噬菌体的基因组产生有活性的子代噬菌体的过程。目前报道的噬菌体基因组的重启策略包括转化细菌宿主重启和基于无细胞体系的重启。
绝大多数噬菌体颗粒中包裹的是线性的基因组DNA，所述线性基因组DNA的末端主要有三种结构，粘性末端、末端重复(DTR)和末端冗余。具体而言，具备上述三种基因组结构特征的噬菌体基因组在复制过程先发生环化，然后复制形成基因组首尾相连的线性串联体DNA，再将线性串联体DNA进行切割并包装到噬菌体颗粒中，形成子代噬菌体。
基因组具有粘性末端或DTR的噬菌体(如lambda、T3和T7等)在包装过程中，对所述线性串联体DNA进行切割的位置是确定的，因此其子代噬菌体颗粒中包裹的基因组具有确定的基因组序列(包括基因组末端结构的序列和长度)，且每个噬菌体颗粒包裹的基因组DNA是同质的DNA分子(一个单位长度基因组)，即基因组数据库(如Genbank) 中列出的基因组序列与其噬菌体颗粒包裹的DNA分子序列一致。目前报道的通过酵母 TAR克隆合成的噬菌体均为这种具有确定基因组序列的噬菌体。
基因组具有末端冗余的噬菌体在包装(headful包装)过程中，对所述线性串联体DNA 进行切割的位置是不确定的，因此其子代噬菌体颗粒中包裹的基因组DNA是异质DNA分子(一个单位长度基因组DNA+不确定的冗余序列)。因此，基因组数据库(如Genbank) 中列出的Headful包装型噬菌体的基因组序列仅为其一个单位长度的基因组DNA序列，而不包含其末端冗余序列。由于Headful包装类型噬菌体颗粒中的DNA分子的异质性，难以确定基因组的末端结构和长度，导致难以实现该类型噬菌体基因组的合成与克隆。例如，目前报道的基于酵母TAR克隆合成DTR噬菌体的合成策略并不能解决Headful 包装型噬菌体的合成问题。
因此，有需要开发一种合成Headful包装型噬菌体的方法，例如，开发一种包含headful包装型噬菌体的基因组的构建体，使其能够在细菌中成功完成基因组重启。
发明内容
本发明多核苷酸，包含第一片段和与所述第一片段的3’端连接第二片段，其中所述第一片段的核苷酸序列是环状重排的Headful包装型噬菌体的单位长度的基因组序列，且其中所述第二片段的核苷酸序列是所述第一片段核苷酸序列的核苷酸1到n，n为至少40。在一些实施方案中，n为至少60。
在一些实施方案中，所述基因组是野生型基因组。在一些实施方案中，所述第一片段的核苷酸序列由SEQ ID NO:13-16之一的核苷酸序列组成。
在一些实施方案中，所述单位长度的基因组是经修饰的基因组。在一些实施方案中，与野生型基因组相比，所述经修饰的基因组包含一或多个核苷酸的插入、缺失、取代或添加。在一些实施方案中，所述第一片段的核苷酸序列是SEQ ID NO:13-16之一的核苷酸序列的经修饰的变体。
本发明还提供一种载体，包含本发明的多核苷酸。
本发明还提供一种细胞，如酵母细胞，包含本发明的多核苷酸或载体。
本发明还提供一种合成Headful包装型噬菌体的方法，包括用本发明的多核苷酸或载体转化所述细菌的步骤。
在一些实施方案中，所述细菌是所述噬菌体的天然宿主。
在一些实施方案中，所述方法还包括在酵母中基于转化相关的重组(TAR)组装所述核酸构建体的步骤。在一些实施方案中，所述酵母是酿酒酵母(Saccharomycescerevisiae)。在一些实施方案中，所述核酸构建体是酵母-细菌穿梭载体。
附图说明
图1显示基于酵母TAR克隆合成Headful包装型噬菌体的方法示意图。
图2A和2B显示构建pYEP-Ⅱ的PCR验证结果。图2A：接口1的菌落PCR电泳结果；图2B：接口2的菌落PCR电泳结果。泳道M：DL 2,000DNA标记；泳道1-8：转化子1-8；泳道N：阴性对照。
图3显示质粒pYEP-Ⅱ及其XhoI和NcoI消化产物电泳结果。泳道M：DL 15,000 DNA标记；泳道1和3：转化子1和3的质粒电泳结果；泳道2和4：转化子1和3的质粒酶切产物电泳结果。
图4显示pYEP-Ⅱ-S4-60-T酵母转化子的PCR接口验证结果。泳道M：DL 1,000DNA标记；泳道1-7：酵母转化子的PCR产物；泳道N：酵母MYA 3666的PCR产物(阴性对照)。
图5显示pRSII313-S4-60-T酵母转化子的PCR接口验证结果。泳道M：DL 2,000 DNA标记；泳道1-8：酵母转化子的PCR产物。
图6显示pYEP-Ⅱ-S4-60-H酵母转化子的PCR接口验证结果。泳道M：DL 2,000 DNA标记；泳道1-8：酵母转化子的PCR产物。
图7显示pRSII313-S4-60-H酵母转化子的PCR接口验证结果。泳道M：DL 2,000 DNA标记；泳道1-8：酵母转化子的PCR产物。
图8显示pYEP-Ⅱ-S4-60-I酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-8：酵母转化子的PCR产物。
图9显示pRSII313-S4-60-I酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-8：酵母转化子的PCR产物。
图10A显示pYEP-Ⅱ-S4-60-T在大肠杆菌中重启后的噬斑检测结果；图10B显示pRSII313-S4-60-T在大肠杆菌中重启后的噬斑检测结果。
图11A显示pYEP-Ⅱ-S4-60-H在大肠杆菌中重启后的噬斑检测结果；图11B显示pRSII313-S4-60-H在大肠杆菌中重启后的噬斑检测结果。
图12A显示pYEP-Ⅱ-S4-60-I在大肠杆菌中重启后的噬斑检测结果；图12B显示pRSII313-S4-60-I在大肠杆菌中重启后的噬斑检测结果。
图13显示pYEP-Ⅱ-S4-60-T在铜绿假单胞菌(Pseudomonas aeruginosa)ATCC9027 中重启后的噬斑检测结果。
图14显示RFP编码序列插入噬菌体合成基因组的示意图。
图15显示pYEP-Ⅱ-S4-60-RFP-T-1酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-16：酵母转化子的PCR产物。
图16显示pYEP-Ⅱ-S4-60-RFP-T-2酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-16：酵母转化子的PCR产物。
图17显示pRSII313-S4-60-RFP-T酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-8：酵母转化子的PCR产物。
图18显示pYEP-Ⅱ-S4-60-RFP-H酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-8：酵母转化子的PCR产物。
图19显示pRSII313-S4-60-RFP-H酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-8：酵母转化子的PCR产物。
图20显示pYEP-Ⅱ-S4-60-RFP-I酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-8：酵母转化子的PCR产物。
图21显示pRSII313-S4-60-RFP-I酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-8：酵母转化子的PCR产物。
图22A显示pYEP-Ⅱ-S4-60-RFP-T-1在大肠杆菌中重启后的噬斑检测结果；图22B显示pYEP-Ⅱ-S4-60-RFP-T-2在大肠杆菌中重启后的噬斑检测结果；图22C显示 pRSII313-S4-60-RFP-T在大肠杆菌中重启后的噬斑检测结果。
图23A显示pYEP-Ⅱ-S4-60-RFP-H在大肠杆菌中重启后的噬斑检测结果；图23B 显示pRSII313-S4-60-RFP-H在大肠杆菌中重启后的噬斑检测结果。
图24A显示pYEP-Ⅱ-S4-60-RFP-I在大肠杆菌中重启后的噬斑检测结果；图24B显示pRSII313-S4-60-RFP-I在大肠杆菌中重启后的噬斑检测结果。
图25A显示RFP序列插入合成噬菌体ORF11下游的验证结果；图25B显示RFP 序列插入合成噬菌体ORF42下游的验证结果。泳道M：DL 2,000DNA标记；泳道1： PCR模板来自野生型噬菌体S4；泳道2：PCR模板来自合成噬菌体S4-RFP-1；泳道3： PCR模板来自合成噬菌体S4-RFP-2；泳道4：PCR模板来自合成噬菌体S4-RFP-3。
图26显示基因组酶切验证结果。泳道M：l kb Extend DNA ladder；泳道1-5：分别为经EcoRI酶切的野生型噬菌体S4以及合成噬菌体S4-Syn、S4-RFP-1、S4-RFP-2和 S4-RFP-3的基因组；泳道6-10：分别为经Eco105I酶切的野生型噬菌体S4以及合成噬菌体S4-Syn、S4-RFP-1、S4-RFP-2和S4-RFP-3的基因组。
图27显示合成噬菌体和野生型噬菌体S4分别与宿主共培养后，培养物的相对光单位(RLU)与时间的关系。
图28显示合成噬菌体与野生型噬菌体S4分别与宿主共培养后，培养物的OD600 与时间的关系。
图29显示pYEP-Ⅱ-S4-0-T酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-16：酵母转化子的PCR产物；泳道N：酵母MYA 3666的菌落PCR产物(阴性对照)。
图30显示pRSII313-S4-0-T的酵母转化子的PCR接口验证结果。泳道M：DL 2,000DNA标记；泳道1-16：酵母转化子的PCR产物。
发明详述
尽管将结合以下所列举的实施方案描述本发明，但是应当理解，它们并不旨在将本发明限制于那些实施方案。相反，本发明旨在覆盖可包括在如权利要求所限定的本发明的范围内的所有替代、修改和等同形式。本领域技术人员将认识到许多与本文描述的方法或材料相似或等同的方法和材料可用于实施本发明。本发明不限于所描述的方法和材料。如果所引用的文献、专利和类似材料中的一个或多个与本申请不同或相矛盾，包括但不限于所定义的术语、术语用法、所描述的技术等，则以本申请为准。除非另有定义，否则本文使用的所有技术和科学术语具有与本发明所属领域的普通技术人员通常理解的相同含义。尽管与本文描述的那些类似或等同的方法和材料可以用于本发明的实践或测试中，但是下面描述了合适的方法和材料。本文提及的所有出版物、专利申请、专利和其他参考文献通过引用整体并入本文。
一、定义
如本文所用，术语“噬菌体”通常是指感染细菌的病毒，具有严格的宿主特异性，并且能够引起宿主菌的裂解。
如本文所用，术语“Headful包装型噬菌体”是指在噬菌体颗粒中的核酸不仅包含单位长度的噬菌体基因组，还包含冗余序列的噬菌体，例如T4、P22和P1噬菌体。
如本文所用，术语“多核苷酸”或者“核酸分子”包括DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)及使用核苷酸类似物产生的DNA或RNA的类似物。所述核酸分子可以是单链或双链的，优选双链DNA。所述核酸的合成可以使用核苷酸类似物或衍生物(例如肌苷或硫代磷酸核苷酸)。这种核苷酸可以用于，例如，制备具有改变的碱基配对能力或者增加的核酸酶抗性的核酸。
如本文所用，术语“编码”是指多核苷酸直接指定其蛋白质产物的氨基酸序列。编码序列的边界一般由“开放读框(ORF)”确定，所述开放读框通常以ATG起始密码子或另外的起始密码子如GTG和TTG开始，以终止密码子如TAA、TAG和TGA结束。所述编码序列可以是DNA、cDNA或重组核苷酸序列。
如本文所用，术语“杂交”是在给定的严格杂交和洗涤条件下，彼此至少大约90％、优选至少大约95％、更优选至少大约96％、更优选至少98％同源的核苷酸序列通常保持彼此杂交。
本领域技术人员知道各种用于杂交的条件，如严格杂交条件和高度严格杂交条件。参见，例如，Sambrook et al.,1989,Molecular Cloning,A Laboratory Manual,ColdSpring Harbor Press,N.Y.；和Ausubel et al.(eds.),1995,Current Protocols inMolecular Biology, John Wiley&Sons,N.Y.。
如本文所用，“核苷酸相同性百分比”或者“核苷酸序列相同性百分比”是指比较两个核酸的核苷酸，当最佳比对时，所述两个多肽具有大约指定的相同核苷酸百分比。例如，“95％的核苷酸相同性”是指比较两个核酸的核苷酸，当最佳比对时，所述两个核酸有95％的核苷酸相同。
对于本发明，为确定两个核酸序列的相同性百分比，以最佳比较为目的比对序列(例如在第一个核酸序列中可导入缺口，以与第二个氨基酸或核酸序列进行最佳比对)。然后比较在相应核苷酸位置的核苷酸。当第一个序列中的位置在第二个序列中相应位置由相同核苷酸占据时，则这些分子在这个位置是相同的。两个序列之间的相同性百分比是所述序列共有的相同位置的数量的函数(即相同性百分比＝相同位置的数量/位置(即重叠位置)的总数量×100)。优选地，这两个序列是相同长度的。本领域技术人员知晓，可以使用不同的计算机程序确定两个序列之间的相同性。
如本文所用，术语噬菌体基因组的“变体”是指与参考噬菌体基因组不同但保留必要特性的多核苷酸。通常，差异是有限的，使得参考基因组和变体的序列总体上非常相似，并且在许多区域是相同的。变体和参考基因组的核苷酸序列可以通过一个或多个修饰(例如，取代，添加和/或缺失)而不同。
如本文所用，术语“环状重排”涉及对初始核酸进行如下设计：将初始核酸的5’端与3’端连接形成环状分子，并在环状分子中的某一位置打开环状分子形成线性分子。
如本文所用，术语“打开位点”是指环状重排的过程中，所生成的线性分子的5’端与3’端的位置。
打开位点可以与连接位点相同或不同。如果在不同于连接位点的位置打开(切割或断裂)所述环状分子，形成新的线性核酸，其具有与初始核酸不同的5’端与3’端；如果在连接位点打开环状分子，则产生与初始核酸相同的核酸。环状重排还包括产生本文所述的环状重排的线性分子的任何过程。通常，环状重排的分子可以是直接构建的线性分子，而不真正经历环化和打开的步骤。当在不同于连接位点的位置打开所述环状分子时，打开位点由位于初始核酸的5’端与3’端之间的一对核苷酸的位置指定，这些核苷酸成为新的线性分子的5’端与3’端。
如本文所用，“野生型”噬菌体是指天然存在的噬菌体，或与天然存在的噬菌体具有相同性质的合成噬菌体。野生型Headful包装型噬菌体的单位长度的基因组可以从网络数据库(如NCBI)获得。
如本文所用，术语“修饰”是指对多核苷酸序列的修饰，包括但不限于，取代、删除、插入和/或添加一或多个核苷酸或氨基酸。修饰也包括不改变多核苷酸或多肽的序列的化学修饰，如多核苷酸的甲基化等。在本文中，经修饰的变体也包括如上所述的环状重排的变体。
二、多核苷酸
本发明提供一种用于合成Headful包装型噬菌体的多核苷酸。
现有技术已知(亦如本申请实施例所证明的)，使用酵母TAR克隆制备的仅包含Headful包装型噬菌体单位长度的基因组的载体不能在宿主菌中成功实现基因组重启。发明人出人意料地发现，在环状重排的Headful包装型噬菌体的单位长度的基因组3’端连接额外的冗余序列，可以在细菌中成功实现基因组重启。
因此，在一些实施方案中，本发明的多核苷酸包含第一片段和与所述第一片段的3’端连接第二片段，其中所述第一片段的核苷酸序列是环状重排的Headful包装型噬菌体的单位长度的基因组序列，且其中所述第二片段的核苷酸序列是所述第一片段核苷酸序列的核苷酸1到n，n为至少40。在一些实施方案中，n为至少60。
在一些实施方案中，所述第二片段的长度小于所述噬菌体的野生型噬菌体颗粒中包装的基因组的冗余序列的长度。本领域技术人员知晓，冗余序列的长度随噬菌体的不同而改变，并且对于给定的噬菌体，冗余序列的长度通常是已知的。例如，在T4、P22和 P1噬菌体中，冗余序列的长度分别是其单位长度的基因组的2％、4％和12％，约900、 1,800和5,400bp。在一些实施方案中，所述第二片段的长度比野生型噬菌体的冗余序列的长度少100bp、200bp、300bp、400bp、500bp、600bp、700bp、800bp、900bp、 1,000bp、1,500bp、2,000bp、3,000bp、4,000bp、5,000bp或更多。
在一些实施方案中，n为40-5000、40-4000、40-3000、40-2000、40-1500、40-1000、40-800、40-600、40-400、40-200、40-100、40-80、60-5000、60-4000、60-3000、60-2000、 60-1500、60-1000、60-800、60-600、60-400、60-200、60-100或60-80。在一些实施方案中，n为40、50、60、70、80、90、100或更多。例如，n为60。
在一些实施方案中，在设计环状重排的单位长度的基因组时，单位长度的基因组的 5’端与3’端直接连接以形成环状分子。
在一些实施方案中，在设计环状重排的单位长度的基因组时，可以选择ORF中的位点作为打开位点。在一些实施方案中，在设计环状重排的单位长度的基因组时，可以选择ORF之间的位点作为打开位点。本领域技术人员会理解，缺失噬菌体基因组中的部分序列，例如非编码和/或非调控序列，可以不影响噬菌体的复制、包装或功能。因此，所述环状重排的单位长度的基因组的5’端与3’端可以是单位长度的基因组中相邻或不相邻的核苷酸。
在一些实施方案中，所述构建体用于合成野生型噬菌体，即所述基因组是野生型基因组。在一些实施方案中，所述噬菌体是vB_PaeS_SCUT-S4。在一些实施方案中，所述第一片段的核苷酸序列由SEQ ID NO:13的核苷酸序列组成。在一些实施方案中，在设计环状重排的单位长度的基因组时，所述打开位点在vB_PaeS_SCUT-S4的ORF45和 ORF46之间、ORF35和ORF36之间或ORF17和ORF18之间。在一些实施方案中，所述打开位点在SEQ ID NO:13的核苷酸37,952和37,953之间、32,385和32,386之间或 11,857和11,858之间。在一些实施方案中，所述第一片段的核苷酸序列由SEQ ID NO: 14-16之一的核苷酸序列组成。
在一些实施方案中，所述第二核苷酸序列包含SEQ ID NO:13-16之一的核苷酸1-n，其中n为至少40或至少60。在一些实施方案中，n为40-5000、40-4000、40-3000、40-2000、40-1500、40-1000、40-800、40-600、40-400、40-200、40-100、40-80、60-5000、60-4000、 60-3000、60-2000、60-1500、60-1000、60-800、60-600、60-400、60-200、60-100或60-80。在一些实施方案中，n为40、50、60、70、80、90、100或更多，例如，n为60。
为了克服细菌宿主对噬菌体的抗性，和/或消除或弱化噬菌体的毒性，可以对噬菌体基因组进行修饰。因此，在一些实施方案中，所述单位长度的基因组是经修饰的基因组。在一些实施方案中，野生型基因组相比，所述经修饰的基因组包含插入、缺失、取代或添加一或多个核苷酸。所述修饰包括但不限于缺失毒力基因的全部或部分、引入取代以弱化毒力基因、插入T-DNA以沉默毒力基因和在抗性相关的基因中引入取代。还可以修饰基因组的非编码区。在一些实施方案中，可以修饰基因组的调控区以调节基因表达。
本领域技术人员会理解，在非编码非调控区进行修饰通常不会影响噬菌体的性质和 /或功能。因此，在一些实施方案中，在噬菌体基因组的非编码非调控区引入修饰。
在一些实施方案中，所述经修饰的基因组与野生型基因组至少70％、80％、90％、95％、96％、97％、98％或99％相同。
在一些实施方案中，所述第一片段的核苷酸序列是SEQ ID NO:13-16之一的核苷酸序列的经修饰的单位长度的基因组。在一些实施方案中，第一片段的核苷酸序列与SEQID NO:13-16之一至少70％、80％、90％、95％、96％、97％、98％或99％相同。
在一些实施方案中，所述第一片段的核苷酸序列是SEQ ID NO:14，且所述第二片段的核苷酸序列是SEQ ID NO:14的核苷酸1-60。在一些实施方案中，所述第一片段的核苷酸序列是SEQ ID NO:15，且所述第二片段的核苷酸序列是SEQ ID NO:15的核苷酸1-60。在一些实施方案中，所述第一片段的核苷酸序列是SEQ ID NO:16，且所述第二片段的核苷酸序列是SEQ ID NO:16的核苷酸1-60。
本领域技术人员会理解，本发明的多核苷酸涵盖单链多核苷酸、双链多核苷酸，例如，如上所定义的多核苷酸的正义链，其中所述第二片段的5’末端与第一片段的3’末端连接；或者与其互补(例如可以与所述正义链杂交，优选在严格条件下杂交)的反义链，其中所述第一片段的反义链的5’末端与所述第二片段的反义链的3’末端连接。
三、载体、细胞和合成Headful包装型噬菌体的方法
为了构建、保存、扩增本发明的多核苷酸，以及用本发明的多核苷酸合成噬菌体，通常需要载体和细胞，如中间宿主细胞。
因此，本发明还提供包含本发明的多核苷酸的载体以及包含所述多核苷酸或载体的细胞，如酵母细胞。
Headful包装型噬菌体的基因组通常超过40kb。因此，本发明的载体是大容量的载体，例如酵母人工染色体(YAC)或细菌人工染色体(BAC)，例如包含酵母复制筛选元件的BAC。
本发明还提供一种合成Headful包装型噬菌体的方法，包括用本发明的多核苷酸或载体转化细菌的步骤。转化细菌的方法是本领域熟知的，例如，热休克法和电转化法。在一些实施方案中，本发明的方法包括用本发明的载体电转化所述噬菌体的宿主细菌的步骤。
在一些实施方案中，所述方法还包括在酵母中基于转化相关的重组(TAR)组装所述多核苷酸的步骤。在一些实施方案中，用于组装所述多核苷酸的载体是YAC或含有酵母复制筛选元件的BAC，所述载体包含酵母的中心粒序列(CEN)、自主复制起始位点 (ARS)和筛选标记(如HIS3、URA3和LEU2)。在一些实施方案中，所述载体是酵母-细菌穿梭载体。
在一些实施方案中，本发明的方法包括
i)在酵母中基于转化相关的重组(TAR)组装多核苷酸，包含第一片段和与所述第一片段的3’端连接第二片段，其中所述第一片段的核苷酸序列是环状重排的Headful包装型噬菌体的单位长度的基因组序列，且其中所述第二片段的核苷酸序列是所述第一片段核苷酸序列的核苷酸1到n，n为至少40或60；
ii)从酵母分离包含所述多核苷酸的载体；和
iii)用所述载体转化细菌细胞进行噬菌体基因组重启。
vB_PaeS_SCUT-S4噬菌体可以感染来自埃希氏菌属(Escherichia，如大肠杆菌)和假单胞菌属(Pseudomonas，如铜绿假单胞菌)的细菌。因此，在一些实施方案中，本发明的方法包括用本发明的多核苷酸或载体转化来自埃希氏菌属或假单胞菌属的细菌，例如大肠杆菌或铜绿假单胞菌，以进行基因组重启，其中所述第一片段的核苷酸序列由SEQ ID NO:13-16之一的核苷酸序列组成，或者与SEQ ID NO:13-16之一至少70％、80％、90％、 95％、96％、97％、98％或99％相同。
在一些实施方案中，本发明的方法包括：
i)在酵母中基于转化相关的重组(TAR)组装多核苷酸，包含第一片段和与所述第一片段的3’端连接第二片段，所述第一片段的核苷酸序列是SEQ ID NO:14，且所述第二片段的核苷酸序列是SEQ ID NO:14的核苷酸1-60；
ii)从酵母分离包含所述多核苷酸的载体；和
iii)用所述载体转化细菌细胞进行噬菌体基因组重启。
在一些实施方案中，本发明的方法包括：
i)在酵母中基于转化相关的重组(TAR)组装多核苷酸，包含第一片段和与所述第一片段的3’端连接第二片段，所述第一片段的核苷酸序列是SEQ ID NO:15，且所述第二片段的核苷酸序列是SEQ ID NO:15的核苷酸1-60；
ii)从酵母分离包含所述多核苷酸的载体；和
iii)用所述载体转化细菌细胞进行噬菌体基因组重启。
在一些实施方案中，本发明的方法包括：
i)在酵母中基于转化相关的重组(TAR)组装多核苷酸，包含第一片段和与所述第一片段的3’端连接第二片段，所述第一片段的核苷酸序列是SEQ ID NO:16，且所述第二片段的核苷酸序列是SEQ ID NO:16的核苷酸1-60；
ii)从酵母分离包含所述多核苷酸的载体；和
iii)用所述载体转化细菌细胞进行噬菌体基因组重启。
可以使用可商购的载体骨架组装本发明的多核苷酸，本发明的载体包含所述载体骨架和本发明的多核苷酸。在一些实施方案中，所述载体骨架是pRSII313(Addgene， #35449)。
也可以根据以上列举的元件构建新的载体骨架来组装本发明的多核苷酸。例如，通过将酵母复制筛选元件CEN-ARS-HIS3引入BAC中以构建所述载体骨架。在一些实施方案中，所述载体骨架包含SEQ ID NO:22的核苷酸序列。
在一些实施方案中，所述酵母是酿酒酵母。在一些实施方案中，基于营养缺陷型标记筛选阳性克隆，其中营养缺陷型标记包括但不局限于HIS3、URA3和LEU2。在一些实施方案中，所述酵母是酿酒酵母ATCC MYA 3666(获自广东省微生物菌种保藏中心)，所述营养缺陷型标记是HIS3。
实施例
通过以下实施例，本领域技术人员会更清楚地理解本发明。应理解，实施例只是用于说明，而非限制本发明的范围。如无特别说明，本发明中使用的实验方法均为常规方法，基因克隆操作具体可参见Sambrook,J.,Russell,David W.,Molecular Cloning:ALaboratory Manual,3rd edition,2001,NY,Cold Spring Harbor。实施例中所使用的引物的合成和测序均由生工生物工程(上海)股份有限公司进行。
实施例1、穿梭质粒pYEP-Ⅱ的构建
以pRSII313(Addgene,#35449)为模板，用引物HL19023(SEQ ID NO:1)和HL19024(SEQ ID NO:2)扩增含有酵母复制筛选元件序列CEN-ARS-HIS3的片段1(SEQ ID NO: 9)。以发明人构建并保存的pBAC shuttle为模板，用引物HL19025(SEQ ID NO:3)和 HL19091(SEQID NO:8)扩增片段2(SEQ ID NO:10)，用引物HL19088(SEQ ID NO:6) 和HL19036(SEQ IDNO:5)扩增片段3(SEQ ID NO:11)，以及用引物HL19089(SEQ ID NO:7)和HL19030(SEQ IDNO:4)分别扩增片段4(SEQ ID NO:12)。PCR反应体系如表 1所示，并以如下步骤进行PCR反应：98℃，30s；98℃，10s，68℃，30s，72℃，1kb/min， 30个循环；72℃，2min。用1％琼脂糖凝胶电泳分离PCR产物，并用凝胶DNA回收试剂盒(Magen)回收PCR产物。使用Nanodrop 2000(Thermo Scientific)测定回收的PCR产物浓度。
表1
将片段1、2、3和4(每个片段的量为30fmol)与15μL Gibson Assembly缓冲液混合，并加入ddH2O使混合物总体积达到20μL。将所述混合物在50℃温育一个小时。
用10μL经温育的混合物转化大肠杆菌EPI 300，通过在含有抗生素(氯霉素25 μg/mL，庆大霉素10μg/mL)的LB平板上，37℃温育12-16h筛选阳性克隆并进行PCR 验证(如图2所示)。挑选菌落PCR验证为阳性的转化子进行培养，使用质粒小提试剂盒 (TIANprep MiniPlasmid Kit，天根生化科技(北京)有限公司，DP103-03)提取质粒pYEP- Ⅱ并将从对应于图2中泳道1和3的克隆提取的质粒用XhoI和NcoI消化并进行凝胶电泳(如图3所示)。对所述质粒进行测序得到目标载体(pYF-II，SEQ ID NO:22)。
实施例2、噬菌体vB_PaeS_SCUT-S4基因组的组装和重启
2.1、制备合成噬菌体所需的DNA片段
选定两个ORF之间的位点作为打开位点，设计噬菌体vB_PaeS_SCUT-S4的(GenBank序列号为MK165658.1，简称S4)的环状重排的单位长度的基因组，并将其拆分为长度为4.8-6.0kb的八个片段(F1-F8)，相邻的两个DNA片段之间的同源臂为60bp，基因组片段F8在3’端连接有片段F1的核苷酸1-60。
以噬菌体vB_PaeS_SCUT-S4的基因组DNA为模板扩增所述片段。
使用pYEP-Ⅱ为载体进行酵母TAR组装时，可以通过两种方法线性化所述载体，包括：i)使用限制性核酸内切酶SphI和HindⅢ对载体线性化；和ii)通过PCR扩增得到线性载体产物，并在扩增产物两端各引入30bp来自片段F1的5’端和片段F8的3’端的序列作为同源臂，并且在片段F1的5’端和片段F8的3’端各引入30bp来自载体的序列作为同源臂。
使用pRSII313为载体进行酵母TAR组装时，采用如上所述的方法ii)。
使用KOD FX DNA聚合酶进行PCR扩增，PCR体系如表2所示，并以如下步骤进行PCR反应：94℃，2min；98℃，10s，60℃，30s，68℃，1kb/min，30个循环。用 1％琼脂糖凝胶电泳分离PCR产物，并用凝胶DNA回收试剂盒(Magen)回收PCR产物。使用Nanodrop 2000(ThermoScientific)测定回收的PCR产物浓度。
表2
2.2、酵母TAR克隆组装
根据制造商的说明书，使用酵母转化试剂盒Frozen-EZ Yeast TransformationII Kit (Zymo research，T2001)制备酵母感受态细胞，步骤如下：
a)挑选经YPD平板活化的酿酒酵母MYA 3666单克隆到约10mL的YPD液体培养基中，于30℃，200rpm振荡培养到中对数生长期(OD600为0.8-1.0)；
b)在500g，室温下离心4分钟收集菌体，弃上清；
c)加入10mL的EZ 1溶液重悬菌体，在500g室温离心4min，弃上清；
d)加入1mL的EZ 2溶液重悬菌体；
e)以100μL每管分装重悬后的菌体，于-80℃保存。
按照以下步骤进行酵母TAR克隆转化：
a)将-80℃保存的酿酒酵母MYA3666的感受态细胞在冰上融化；
b)将组装所需要的噬菌体基因组DNA片段(每个片段200ng)和载体(100ng)加入到100μL酵母感受态细胞中，同时加入500μL EZ 3溶液，充分混匀；
c)于30℃孵育2h，每间隔半小时用手或涡旋仪轻轻振荡；
d)在500g室温离心4min，弃部分上清后重悬菌体，均匀地涂布在SD-HIS平板上，于30℃倒置培养2-4天。
经酵母TAR克隆组装后得到的含有噬菌体S4合成基因组序列(环状重排的单位长度的基因组+60bp的冗余序列)的酵母质粒信息如表3所示。
表3
2.3、酵母转化子接口验证
挑取转化后平板上的单克隆到20μL 25mM的NaOH溶液中，在95℃孵育30min 以裂解细胞，然后短暂离心后取1μL上清作为PCR模板，通过PCR进行接口验证PCR 体系如表4所示，PCR步骤如实施例2.1所示，采用凝胶电泳验证PCR产物。
表4
组装pYEP-Ⅱ-S4-60-T的酵母转化子的部分验证结果如附图4所示，所有接口的阳性率为5/16；组装pRSII313-S4-60-T的酵母转化子的验证结果如附图5所示，所有接口的阳性率分别为4/8；pYEP-Ⅱ-S4-60-H的酵母转化子的验证结果如附图6所示，所有接口的阳性率为8/8；组装pRSII313-S4-60-H的酵母转化子的验证结果如附图7所示，所有接口的阳性率分别为5/8；pYEP-Ⅱ-S4-60-I的酵母转化子的验证结果如附图8所示，所有接口的阳性率为2/8；组装pRSII313-S4-60-I的酵母转化子的验证结果如附图9所示，所有接口的阳性率分别为3/8。
2.4、酵母质粒提取
对验证为阳性酵母转化子进行培养，并按照以下步骤提取酵母质粒：
(1)将阳性单克隆在10mL的SD-HIS液体培养基中培养36-48h；
(2)在室温4,000rpm离心5min，弃上清，并用500μL P1溶液(Tiangen)重悬菌体，加入2μL的β-巯基乙醇和20μL 10mg/mL的zymolyase-20T，于37℃、220rpm振荡 2-3h；
(3)加入60μL 2％的SDS溶液，于70℃孵育15min，加入60μL预冷(冰上)的5M 乙酸钾，混匀并轻轻振荡，冰浴5min后于12,000g离心10min，转移上清到新的离心管中；
(4)加入600μL的异丙醇并混匀，冰浴5min后12,000g离心15min，弃上清；
(5)用600μL预冷(冰上)的70％乙醇洗涤沉淀三次，室温干燥DNA沉淀后用30μL 预热(37℃)的去离子水溶解DNA沉淀。
2.5、酵母质粒电转化大肠杆菌及噬斑检测
按照以下步骤将实施例2.4中提取的酵母质粒转化大肠杆菌并进行噬斑检测：
(1)将10μL酵母质粒溶液与100μL的大肠杆菌NEB 10-beta(博迈德生物)电转感受态细胞混匀，冰浴约5min；
(2)缓慢地将上述混合液转移到预冷(冰)的2mm电击杯中，避免产生气泡，将电击杯插在冰中；
(3)在电压2.5kV、电容25μF、电阻200Ω进行电转，电转后立即加入800μL预热(37℃)的SOC培养液，充分混匀后将电击杯中的液体转移到1.5mL离心管中，放入 37℃，220rpm振荡培养2h；
(4)加入10μL的氯仿，轻轻震荡并静置10min，然后在12,000g、室温下离心2min。转移上清到新的离心管中，并加入300μL过夜培养的宿主铜绿假单胞菌ATCC 9027菌液混匀，静置15min。然后将混合液与5mL预热(55℃)的琼脂浓度为0.6％的上层LB 培养基充分混匀，倒在琼脂浓度为1.5％的LB平板上，待冷却凝固后于37℃培养箱倒置培养过夜。
图10显示用pYEP-Ⅱ-S4-60-T(图10A)或pRSII313-S4-60-T(图10B)转化的大肠杆菌NEB 10-beta中的重启后噬斑检测的结果；图11显示用pYEP-Ⅱ-S4-60-H(图11A)或pRSII313-S4-60-H(图11B)转化的大肠杆菌NEB 10-beta中的重启后噬斑检测的结果；图12显示用pYEP-Ⅱ-S4-60-I(图12A)或pRSII313-S4-60-I(图12B)转化的大肠杆菌NEB 10-beta中的重启后噬斑检测的结果。
结果显示，对于6种含有噬菌体S4合成基因组序列(SEQ ID NO:14-16)的酵母质粒，经转化的大肠杆菌裂解物的上清与噬菌体宿主在双层琼脂平板上共培养后均产生了噬菌斑，表明了6种质粒转化到大肠杆菌后均成功重启。
2.6、酵母质粒电转化铜绿假单胞菌及噬斑检测
按照以下步骤将实施例2.4中提取的酵母质粒pYEP-Ⅱ-S4-60-T转化铜绿假单胞菌ATCC 9027，并进行噬斑检测：
(1)将10μL酵母质粒溶液与100μL铜绿假单胞菌ATCC 9027的电转感受态细胞混匀，冰浴约5min；
(2)缓慢地将上述混合液转移到预冷(冰)的2mm电击杯中，避免产生气泡，将电击杯插在冰中；
(3)在电压3.0kV、电容25μF、电阻400Ω进行电转，电转后立即加入800μL预热的SOC培养液，充分混匀后将电击杯中的液体转移到1.5mL离心管中，在37℃，220 rpm振荡培养2h；
(4)在生物安全柜中将培养液与5mL预热的琼脂浓度为0.6％的上层LB培养基充分混匀，倒在琼脂浓度为1.5％的LB平板上，待冷却凝固后于37℃恒温培养箱倒置培养过夜。
噬斑检测结果如附图13所示，在双层琼脂平板上观察到了噬斑，表明pYEP-Ⅱ -S4-60-T质粒转化到噬菌体的宿主铜绿假单胞菌ATCC 9027中后噬菌体成功重启。
实施例3、经修饰的噬菌体vB_PaeS_SCUT-S4合成基因组的组装和重启
3.1、噬菌体vB_PaeS_SCUT-S4经修饰的合成基因组的组装
扩增含有红色荧光蛋白(RFP)编码序列(SEQ ID NO:17)的核苷酸序列，并将其插入噬菌体基因组ORF11(编码主要衣壳蛋白)或ORF42(编码假定的蛋白)的终止密码子下游(如图14所示)。在扩增该序列时利用引物在其5’和3’末端分别引入插入位点上下游各30bp的序列作为同源臂。其他片段的设计和扩增，以及包含RFP编码序列的经修饰的合成基因组的组装以类似于实施例2.1和2.2的方法进行，质粒信息如表5所示。
表5
3.2、噬菌体vB_PaeS_SCUT-S4重组基因组的验证与重启
按照实施例2.3中的接口验证方案验证上述酵母TAR克隆转化后的转化子，pYEP-Ⅱ-S4-60-RFP-T-1的验证结果如图15所示，阳性率为12/16；pYEP-Ⅱ-S4-60-RFP-T-2 的验证结果如图16所示，阳性率分别为15/16；pRSII313-S4-60-RFP-T的验证结果如图 17所示，阳性率分别为5/8；pYEP-Ⅱ-S4-60-RFP-H的验证结果如图18所示，阳性率分别为7/8；pRSII313-S4-60-RFP-H的验证结果如图19所示，阳性率分别为5/8；pYEP- Ⅱ-S4-60-RFP-I的验证结果如图20所示，阳性率分别为5/8；和pRSII313-S4-60-RFP-I 的酵母转化子的验证结果如图21所示，阳性率分别为5/8。
按照实施例2.4中描述的方法提取酵母质粒，并按照实施例2.5中描述的方法电转化大肠杆菌并进行噬斑检测。图22显示用pYEP-Ⅱ-S4-60-RFP-T-1(图22A)、pYEP-Ⅱ -S4-60-RFP-T-2(图22B)和pRSII313-S4-60-RFP-T(图22C)转化的大肠杆菌NEB 10-beta 中重启后噬斑检测的结果；图23显示用pYEP-Ⅱ-S4-60-RFP-H(图23A)和 pRSII313-S4-60-RFP-H(图23B)转化的大肠杆菌NEB 10-beta中重启后噬斑检测的结果；图24显示用pYEP-Ⅱ-S4-60-RFP-I(图24A)和pRSII313-S4-60-RFP-I(图24B)转化的大肠杆菌NEB 10-beta中重启后噬斑检测的结果。
结果显示，七种质粒转化的大肠杆菌裂解物的上清与噬菌体宿主在双层琼脂平板上共培养后均产生了噬斑，表明七种包含经修饰的合成基因组的酵母质粒转化到大肠杆菌后均成功重启。
实施例4、合成噬菌体的验证及表征
对实施例2和实施例3中得到的部分合成噬菌体(如表6所示)进行验证和表征，验证合成噬菌体的引物如表7所示。
表6
表7
4.1、合成噬菌体的验证
将单个噬斑重悬于1mL的SM缓冲液中，10,000g离心后将上清加到10mL生长到对数生长期的铜绿假单胞菌ATCC 9027的培养液中，37℃，220rpm培养过夜。然后加入100μL的氯仿，37℃培养15min后于4,000rpm，4℃离心15min去除菌体碎片，将上清用0.2μm的滤膜过滤后转移到新的离心管中，加入50μL的氯仿于4℃保存。
使用SM缓冲液将扩增后的合成噬菌体以10倍梯度稀释，直至1010倍。然后，在装有0.3mL过夜培养的宿主菌液的离心管中分别加入0.1mL稀释108、109、1010倍的噬菌体稀释液，静置15min后加入到5mL预热的0.6％LB上层琼脂中，混匀后倒在提前标记好的1.5％LB平板上，待凝固后倒置于37℃培养过夜，然后计算噬斑数目，按下式计算效价：
提取合成噬菌体的基因组，并作为模板进行PCR检测，分别采用引物对HL20108 和HL20109、引物对HL20137和HL20138验证合成噬菌体S4-RFP-1、S4-RFP-2和 S4-RFP-3的基因组上的RFP序列，对PCR产物采用DNA凝胶电泳分析。
PCR产物验证的电泳结果如附图25所示，当用引物对HL20108和HL20109检测时，从RFP编码序列位于合成噬菌体基因组的ORF11下游的合成噬菌体S4-RFP-1和合成噬菌体S4-RFP-2扩增到的条带是1372bp，而从野生型噬菌体S4和合成噬菌体 S4-RFP-3扩增到的条带是589bp(图25A)；当用引物对HL20137和HL20138RFP检测时，从RFP编码序列位于序列位于合成噬菌体基因组的ORF42下游的合成噬菌体 S4-RFP-3扩增到的条带是1239bp，而从野生型噬菌体S4、合成噬菌体S4-RFP-1和合成噬菌体S4-RFP-2扩增到的条带是456bp(图25B)。
对合成噬菌体S4-syn、S4-RFP-1、S4-RFP-2和S4-RFP-3的基因组采用限制性内切酶EcoRI和Eco105I进行酶切验证，并以野生型噬菌体的基因组酶切产物为对照，对酶切产物进行凝胶电泳分析。
结果如图26所示，合成噬菌体S4-Syn和野生型噬菌体S4的基因组被两种不同的内切酶消化后的条带一致。合成噬菌体S4-RFP-1、S4-RFP-2和S4-RFP-3的基因组酶切后的结果显示插入RFP序列导致的差异条带(图26中箭头所述)，其它条带与野生型一致。
4.2、合成噬菌体的表征
将过夜培养的铜绿假单胞菌ATCC 9027用新鲜的LB液体培养基稀释到108cfu/mL，加入108pfu/mL的合成噬菌体(以野生型噬菌体S4作为对照)，混匀后将样品加入到96 孔板中，置于光栅型多功能酶标仪(Infinite M200 PRO，TECAN)中，37℃、240rpm震荡培养，每隔10min检测RFP的荧光强度(激发波长为588nm，发射波长为635nm，增益值为100％)和OD600的变化，连续检测24h，每种噬菌体三个重复。
以感染时间为横坐标，分别以RLU和OD600为纵坐标，检测合成噬菌体感染宿主后RFP的表达量和OD600的变化趋势。
感染时间与RLU的变化关系如附图27所示，插入RFP序列的合成噬菌体S4-RFP-1、S4-RFP-2和S4-RFP-3在与宿主共培养24小时后RLU的数值显著高于不含RFP序列的合成噬菌体S4-Syn以及野生型噬菌体S4。合成噬菌体S4-Syn与野生型噬菌体S4的RLU 数值几乎一致。RFP序列位于ORF11后的合成噬菌体S4-RFP-1和S4-RFP-2的RLU数值几乎一致，RFP序列位于ORF42后的合成噬菌体S4-RFP-3的RLU数值显著低于RFP 序列位于ORF11后的合成噬菌体S4-RFP-1和S4-RFP-2。
感染时间与OD600的变化关系如附图28所示。四株合成噬菌体与宿主共培养后OD600随时间的变化趋势和野生型噬菌体S4与宿主共培养后OD600随时间的变化趋势几乎一致，说明合成噬菌体对宿主的杀伤能力与野生型噬菌体S4无明显差别。
实施例5、噬菌体vB_PaeS_SCUT-S4基因组的组装和重启
根据与实施例2类似的方法设计噬菌体S4的合成基因组，其中所述合成基因组仅包含环状重排的单位长度的基因组SEQ ID NO:14，而不包含所述冗余序列，并分别组装入pYEP-II和pRSII313载体，得到质粒pYEP-Ⅱ-S4-0-T和pRSII313-S4-0-T。
按照实施例2中的接口验证方案验证上述酵母TAR克隆的转化子，pYEP-Ⅱ-S4-0-T的酵母转化子的验证结果如图29所示，阳性率为6/16；pRSII313-S4-0-T的酵母转化子的验证结果如图30所示，阳性率分别为14/16。
按照实施例2的方法从阳性克隆提取酵母质粒，并用酵母质粒电转化大肠杆菌及进行噬斑检测。结果显示pYEP-Ⅱ-S4-0-T和pRSII313-S4-0-T转化的大肠杆菌NEB 10-beta中进行基因组重启，在噬斑检测中均未观察到噬斑形成，表明没有冗余序列的合成基因组不能在细菌中成功重启。
实施例6、包含噬菌体S4合成基因组的质粒在大肠杆菌中的稳定性
根据实施例2的方法，用酵母质粒pYEP-Ⅱ-S4-60-T电转化大肠杆菌NEB 10-beta，在孵育两个小时后10,000g离心并弃部分上清，重悬菌体沉淀后将菌液均匀的涂布在含有抗生素(氯霉素25μg/mL，庆大霉素10μg/mL)的LB固体培养基的平板上，于37℃倒置培养过夜。
结果显示，在含有抗生素的LB固体培养基的平板上未观察到转化子。结果表明，酵母质粒pYEP-Ⅱ-S4-60-T在大肠杆菌中不能稳定存在。
发明人认为，可能是噬菌体S4的基因组表达产物(如holin和endolysin等蛋白)对大肠杆菌有毒害。
序列表
<110> 华南理工大学
<120> 一种合成Headful包装型噬菌体的方法
<130> TC5282
<160> 22
<170> PatentIn version 3.5
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agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt actcttggcc 900
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ctaaaaaatg ttgtacgtga aacggatgaa gttggtaaag gtcagatccg gatgagaact 1260
gtttttgaac aggccattga tcaacgctct tcaactggtg cctggagaaa tgctctttct 1320
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agataatgaa gcgtgcgcct gttattccaa aacatacgct caatactcaa ccggttgaag 1440
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ttgaagtgct ccggggtgat agtgttgaga agacctctcg ggtatggtca ggtaatgaac 1620
gtgaccagga gctgcttact gaggacgcac tggatgatct catcccttct tttctactga 1680
ctggtcaaca gacaccggcg ttcggtcgaa gagtatctgg tgtcatagaa attgccgatg 1740
ggagtcgccg tcgtaaagct gctgcactta ccgaaagtga ttatcgtgtt ctggttggcg 1800
agctggatga tgagcagatg gctgcattat ccagattggg taacgattat cgcccaacaa 1860
gtgcttatga acgtggtcag cgttatgcaa gccgattgca gaatgaattt gctggaaata 1920
tttctgcgct ggctgatgcg gaaaatattt cacgtaagat tattacccgc tgtatcaaca 1980
ccgccaaatt gcctaaatca gttgttgctc ttttttctca ccccggtgaa ctatctgccc 2040
ggtcaggtga tgcacttcaa aaagccttta cagataaaga ggaattactt aagcagcagg 2100
catctaacct tcatgagcag aaaaaagctg gggtgatatt tgaagctgaa gaagttatca 2160
ctcttttaac ttctgtgctt aaaacgtcat ctgcatcaag aactagttta agctcacgac 2220
atcagtttgc tcctggagcg acagtattgt ataagggcga taaaatggtg cttaacctgg 2280
acagatctcg tgttccaact gagtgtatag agaaaattga ggccattctt aaggaacttg 2340
aaaagccagc accctgatgc gaccacgttt tagtctacgt ttatctgtct ttacttaatg 2400
tcctttgtta caggccagaa agcataactg gcctgaatat tctctctggg cccactgttc 2460
cacttgtatc gtcggtctga taatcagact gggaccacgg tcccactcgt atcgtcggtc 2520
tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga 2580
ccacggtccc actcgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta 2640
tcgtcggtct gattattagt ctgggaccat ggtcccactc gtatcgtcgg tctgattatt 2700
agtctgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg aaccacggtc 2760
ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg tatcgtcggt 2820
ctgattatta gtctgggacc acgatcccac tcgtgttgtc ggtctgatta tcggtctggg 2880
accacggtcc cacttgtatt gtcgatcaga ctatcagcgt gagactacga ttccatcaat 2940
gcctgtcaag ggcaagtatt gacatgtcgt cgtaacctgt agaacggagt aacctcggtg 3000
tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc ttatccacaa cattttgcgc 3060
acggttatgt ggacaaaata cctggttacc caggccgtgc cggcacgtta acttggtaac 3120
g 3121
<210> 11
<211> 2670
<212> DNA
<213> Artificial Sequence
<220>
<223> 载体片段
<400> 11
gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 60
cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 120
gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 180
aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 240
caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 300
ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcggtacc cggggatcct 360
ctagagtcga cctgcaggca tgcaagcttg agtattctat agtgtcacct aaatagcttg 420
gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 480
aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 540
acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 600
cattaatgaa tcggccaacg cgaacccctt gcggccgccc gggccgtcga ccaattctca 660
tgtttgacag cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc 720
gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg 780
ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca 840
aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata 900
tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa 960
actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt 1020
agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa 1080
ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg 1140
gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc 1200
catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata 1260
aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt 1320
ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca 1380
ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc 1440
tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg 1500
aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg 1560
cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac 1620
aggtatttat tcgcgataag ctcatggagc ggcgtaaccg tcgcacagga aggacagaga 1680
aagcgcggat ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg 1740
ccgccgctgc tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc 1800
attcctatgc gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga 1860
tgggacataa gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg 1920
ctgcccggca ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac 1980
aattatcctg agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg 2040
ctgtttttgt ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag 2100
tcgggaaaat ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc 2160
gtttatagga agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat 2220
atgccttcag gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg 2280
tcagcaatgg acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca 2340
gccagtagtg ctcgccgcag tcgagcgaca gggcgaagcc aagagtttgt agaaacgcaa 2400
aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt tatggcgggc 2460
gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat 2520
ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt 2580
tcgactgagc ctttcgtttt atttgatgcc tggttaggtg gcggtacttg ggtcgatatc 2640
aaagtgcatc acttcttccc gtatgcccaa 2670
<210> 12
<211> 3676
<212> DNA
<213> Artificial Sequence
<220>
<223> 载体片段
<400> 12
gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt 60
aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgaggagat 120
tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg agatcataga 180
tatagatctc actacgcggc tgctcaaacc tgggcagaac gtaagccgcg agagcgccaa 240
caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc 300
cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac 360
cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc 420
gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa 480
catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg 540
taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa 600
gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag 660
ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaat 720
tcgctagagt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg 780
cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg 840
atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg 900
ccgacggcca gcaggtaggc cgacaggctc atgccggctg ccgccgcctt ttcctcaatc 960
gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc 1020
ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct 1080
cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa 1140
cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac 1200
caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga 1260
tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgctgc 1320
ttccctgctg ttttgtggaa tatctaccga ctggaaacag gcaaatgcag gaaattactg 1380
aactgagggg acaggcgaga gacgatgcca aagagctaca ccgacgagct ggccgagtgg 1440
gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc gttcctggcg 1500
gtgagggcgg atgtcgaccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg 1560
cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa 1620
agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct 1680
ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc 1740
actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa 1800
gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc 1860
gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa 1920
tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac 1980
gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg 2040
gccggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt gcagggccat 2100
agacggccgc cagcccagcg gcgagggcaa ccagcccggc tcgagtgagc gaggaagcac 2160
cagggaacag cacttatata ttctgcttac acacgatgcc tgaaaaaact tcccttgggg 2220
ttatccactt atccacgggg atatttttat aattattttt tttatagttt ttagatcttc 2280
ttttttagag cgccttgtag gcctttatcc atgctggttc tagagaaggt gttgtgacaa 2340
attgcccttt cagtgtgaca aatcaccctc aaatgacagt cctgtctgtg acaaattgcc 2400
cttaaccctg tgacaaattg ccctcagaag aagctgtttt ttcacaaagt tatccctgct 2460
tattgactct tttttattta gtgtgacaat ctaaaaactt gtcacacttc acatggatct 2520
gtcatggcgg aaacagcggt tatcaatcac aagaaacgta aaaatagccc gcgaatcgtc 2580
cagtcaaacg acctcactga ggcggcatat agtctctccc gggatcaaaa acgtatgctg 2640
tatctgttcg ttgaccagat cagaaaatct gatggcaccc tacaggaaca tgacggtatc 2700
tgcgagatcc atgttgctaa atatgctgaa atattcggat tgacctctgc ggaagccagt 2760
aaggatatac ggcaggcatt gaagagtttc gcggggaagg aagtggtttt ttatcgccct 2820
gaagaggatg ccggcgatga aaaaggctat gaatcttttc cttggtttat caaacgtgcg 2880
cacagtccat ccagagggct ttacagtgta catatcaacc catatctcat tcccttcttt 2940
atcgggttac agaaccggtt tacgcagttt cggcttagtg aaacaaaaga aatcaccaat 3000
ccgtatgcca tgcgtttata cgaatccctg tgtcagtatc gtaagccgga tggctcaggc 3060
atcgtctctc tgaaaatcga ctggatcata gagcgttacc agctgcctca aagttaccag 3120
cgtatgcctg acttccgccg ccgcttcctg caggtctgtg ttaatgagat caacagcaga 3180
actccaatgc gcctctcata cattgagaaa aagaaaggcc gccagacgac tcatatcgta 3240
ttttccttcc gcgatatcac ttccatgacg acaggatagt ctgagggtta tctgtcacag 3300
atttgagggt ggttcgtcac atttgttctg acctactgag ggtaatttgt cacagttttg 3360
ctgtttcctt cagcctgcat ggattttctc atactttttg aactgtaatt tttaaggaag 3420
ccaaatttga gggcagtttg tcacagttga tttccttctc tttcccttcg tcatgtgacc 3480
tgatatcggg ggttagttcg tcatcattga tgagggttga ttatcacagt ttattactct 3540
gaattggcta tccgcgtgtg tacctctacc tggagttttt cccacggtgg atatttcttc 3600
ttgcgctgag cgtaagagct atctgacaga acagttcttc tttgcttcct cgccagttcg 3660
ctatcacgtg ctataa 3676
<210> 13
<211> 42932
<212> DNA
<213> s4 phage
<400> 13
atgaacgaac aacaattttt taacgtagcc gtcgcaatta tcggcgcgtt gggcggttgg 60
tggatgcgcg ttatgtggca gtcacttaaa gaacttcaag cgcaagattc gaagttggcc 120
gataaagtcg gcaatatcga agttcttgta gccgggcagt atgtcaagcg cgacgatatg 180
aaccgcgaca taatggcgat tttcgccaaa cttgaccgca tcgaagacaa gatagacaaa 240
aaggccgaca aatgaagaag cccgaactta tcgaagattg gcgcgaatgc tggcgctttt 300
ggtcggttcg actggccgca gtcggcaccg caattactgg ccttcttgtt gcgtttcccg 360
acgcagcgtt ggccgcttgg gcaatccttc cgcacgaatt gaagtcgtcg attccgccgc 420
agtatatgcc gcttatcggc gttgttgttt tcgcgctttc catcgttgcc cgccttgtca 480
agcaaaagaa cttgaacaag gggccgaagg atgaataacg aacttccttg ggtagccgaa 540
gcccgaaagt atatcggcct tcgcgaagac acttcgaaga cttcgcataa cccgaagctt 600
cttgccatgc ttgaccgcat gggcgaattt tccaacgaat cccgcgcttg gtggcatgac 660
gacgaaacgc cttggtgcgg tctgttcgtc ggctattgct tgggcgtagc cgggcgctac 720
gtcgtccgcg aatggtacag ggcgcgggca tgggaagccc cgcagcttac caagcttgac 780
cggcccgcat acggcgcgct tgtgaccttc acgcgaagcg gcggcggcca cgtcggtttt 840
attgtgggca aggatgcgcg cggtaatctt atggttcttg gcggtaatca gtcgaacgcc 900
gtaagtatcg caccgttcgc agtatcccgc gtaaccggct atttctggcc gtcgttctgg 960
cgaaacaaga ccgcagttaa aagcgttccg ttcgaagaac gttattcgct gccgctgttg 1020
aagtcgaacg gcgaactttc gacgaatgaa gcttaaaggg gcatcgcatg ttttccgtaa 1080
tttgggccaa gtttggcaaa tggcttgccg caatcggcgc gggtcttgtc atgctggcaa 1140
gtatttttta tgccggtcgc aagtccggcg caaccaaggc cgaagccaac gcagcaaacg 1200
aacgggcaaa cgaccgcgaa gcaatcgcag ttcgccaagt taacgaagcc cgcgaagctt 1260
ccgaacaaca ggtaaaggcg gtacaaaatg cgaaagacgt tgcttccgat aattctactt 1320
ttgacgacga cgaagttact aagcggctgc gcgacgaatg gtcgcgcgac tgaaccgcaa 1380
agcatcgccg acgcagtgca aacgaagccc gttgtaatcg acacggcttg caaatgggtt 1440
gcgcctattt ggatttcgaa ggccgacggc ttgaccgctg gcacggcccg gcaaattctg 1500
aaccataacg aagccgtcga acgcaattgc gggccgcagt cgccgcctaa atctgcggac 1560
aagccttaac agaacgcggg gcttgcggta tagtgaagcc aacgacgtat aaactaggcc 1620
gacactatgt ccgacgaact gaaagaaagc gaagaaaagg ccgcatacgc ggcccttctt 1680
ttgaaagaac gcgacccgtt caaggctgcg ctttcgctgt ttccgaacaa cacgaatcgc 1740
gcgctatggg ttgcgaacca ttggccgaac gacgccgaag ttaaggccga acaaaagcgc 1800
cttaccgacg aaggcggcga tatggcattt cttccgggca aaggcgacct tgcccgcgac 1860
atttggcagc gtatgcaagg tacgacgctt ccgaacggcg taaccattcc gccgacgccc 1920
gaagaatacg cgaagcttgc caagctttac gccgacgttc gcgggttcat cgaaaaaccg 1980
caaacgaacg ttaacgtaac gacgaacgta aaccgcgtcg tcgaaatgcc cgtattcgct 2040
aacgaaagcg aatgggaagc ggaagccgca cggcaacagc gcgaattgtt agaaaatgca 2100
cgcactcgcc attgatacga acggggcgaa acctgttccg tatgaagtcg tatttaagcc 2160
ccttccgggg tcgcagacta tcgccctttg ttcgatggca gcgcatacgc tgtacgaagg 2220
cgcacgcggc cccggtaaga cgcttacgca gcttatgcgc ttttatcgta acgtcggcaa 2280
aggctacggc aagttctggc gcggcgttat cttcgatttg gaatttgacc atttgggcgg 2340
ccttgttgcc gaatcgaaaa aatggttcgg cgataacgga aagctaaaag acggcggcaa 2400
gttttacgaa tcaacgtcgg cttataaatg ggtttggccg acaggcgaag aactgttgtt 2460
tcggcacgtt aagaagctgt cggattacga aggcttccac ggtcacgaat acccgtttat 2520
cggttggaac gaacttacga agcatccaag cggcgacctt tacgacaaat ttatgtcggt 2580
caaccgctgt acgttcgacc cgataaaaga cacgccgaaa gacccgaaaa ccgggcgcta 2640
tttgacgccg aacggcgaac cgctgccgcc tgtaaagtgc gaagtattca gcacaacgaa 2700
cccaagcggc cccggtcaca attgggtaaa acgtcgcttt attacgattg ccccgcgcgg 2760
caccgtagtt cgtcgcgaaa ttcagattta caacccggcg accgaaaaag aagaaacgca 2820
cgtaattagc caaatcgcta tcttcggttc gtataaggaa aacccgtacc ttccggcttc 2880
gtatatcgcc gaactggaaa gtatcaaaga accgaacttg cggaaggctt ggctttacgg 2940
cgattgggac gttaccgcag gcggcgcaat cgacgacctt tggcaatcgc atatacacgt 3000
tgtaccgcgc ttcgtcattc cgccaagctg gcgaatcgac agaacatacg acgacgggtc 3060
ttcgcatccg tttagtgtgg gctggtgggc ggaagcggac ggcaccgaag cgaccatagt 3120
tctttcggat ggcaccgaat ttacgttttg cccgcaaccg ggttcgcttg ttcagttgtt 3180
cgaatggtac ggatgcgcga aagacgaaaa aggcgaatac ataccgaata aaggtttgaa 3240
gctttcggcg tcgaatatcg cacaaggtat aatcgaccgc gaaatttcgc taatggcgaa 3300
cggttggatt tattcgcagc cttggcccgg ccctgccgac aaccgcattc gccaagtaat 3360
cgacagcgaa ctagacacga ccgaaaagct tatgtcgaag aagggcgttc gttggacgga 3420
atcggacaaa tcgccgggtt cgcgcgtaat cgggttgcag cttttccgcg accggctaga 3480
agcgtctgtt aatcgcgaag ggccgggtat atactttatg tcgaattgcg ttgcaagtat 3540
tgatttgctg cccacactgc cgcgcgacga aaagaagatt gacgacgtag acacgaccgc 3600
cgaagaccat tgttacgata tggtgcgtta tcgcgtattg aaaggcgcga acaaagcggc 3660
agcgaaattc aaacttgtta tgccaactta aaggaaccca aatcatgccg aacgtttcat 3720
ttattcgccc cgaacttggt aagctgctgc cgctgtatta cctgattcgc gacgcaatcg 3780
cgggcgaacc aacggtaaaa ggcgcgcgga cgacttatct tccgatgccg aacgccgaag 3840
accaatcgaa agaaaataag gcgcgatacg aagcgtactt gaagcgggcc gttttctata 3900
acgtagcccg tcgaaccttg ttcggcttgg tcggtcaagt gttcatgcgc gacccggttg 3960
taaaggttcc cgcgctgttg aacccgcttg tcgcaaacgc gacgggttcg ggtatcaacc 4020
ttacgcagct tgcgaaaaag gccgtatcgc tgaaccttgc gtattcccgc gccgggctgt 4080
tggtcgatta cccgacgacc gaagccgaag gcggcgcgtc gattgccgac cttgaagccg 4140
ggcgcattcg tccgaccctg tacgtatatt cgccgaccga aattatcaat tggcgaacga 4200
ccgaccgggg cgcggaagaa gttttatcgt tggtcgtact gttcgaaact tggtgcgctg 4260
ccgatgatgg ttttgaaatg aaaacgtcgg ggcaattccg cgttttgcgt ttggacgaag 4320
aaggttatta cgtccacgaa atttggcgcg aaccgcagcc gaccaaagcc gacggtacga 4380
agattccgaa aggcaattac caacaataca cggtttacaa gccgacggac gcaaacggca 4440
atcgtcttac cgaaattccg tttatgttca tcggttcgga aaacaacgat tcgaacccgg 4500
acaacccgaa cttttacgac cttgcgtcgc tgaatatggc gcattatcgc aattcggcgg 4560
attacgaaga aagttgttat atcgtcggac agccgacgcc ggttcttatc ggacttaccg 4620
aagaatgggt tacgaacgtt cttaaaggtt ccgttaactt cggttcgcgc ggcggtattc 4680
cgcttcctgc cggggccgac gcgaagttgc ttcaagcatc cgaaaacacc atgcttaaag 4740
aagcaatgga taccaaagaa cgccaaatgg tcgcacttgg cgcaaagctt gtcgaacaaa 4800
aggaagtgca acgcacggca accgaagccg aattggaagc cgcttcggaa ggttcgacgc 4860
tttccagtgc aaccaagaac gtttcggcgg cctttgaatg ggcgttgaaa tgggcggccc 4920
gttgggtcgg tcaagccgac agcggcgtta agttcgaact gaatacagat ttcgacattg 4980
cccgcatgac gccggaagaa cgccgttcgc ttgttgaaga atggcagaaa ggcgcaatta 5040
ccttcgaaga aatgcgaacc ggcttgcgta aagctggcgt cgcaaccgaa gacgacgcca 5100
aggctaaaga aaaaatcgcc aaggataccg ccgaagcaat ggcccttgct gccccggcta 5160
acgtgccggg cgatggcagc ggcggcgata atgtgggcaa caacggataa ggggcgcaat 5220
catggcacta tcggacaata agcgactgta cgatattgcg acgcgccttg ctgtttacgt 5280
cgaagacgtg aaagtttggc agtcccgaca attcgggttc gtacttcgcg aagtaaactt 5340
ggaattaacg cgccttcttg gtcgtgttcg ttataagacc cttgacggtc tttcgaaggc 5400
gcagttaaac aagcttgttt cggaattacg ggaatcgcaa tcgaagattt acagcgcata 5460
cacgcaaacg ctgcttgaac agttaaagga attcatgcgg gccgacttgg aagtaaaccg 5520
gcgcgcttgg gttacgggtt atatcgaact tgacggcgaa gaaagcgacg acattatttc 5580
ggacgaagaa gcgatacagt ttcttttaga agtaccaaac gccgattcga accctttgtt 5640
cggtttggcc gctgttacag gaagcgacga acgcatttgg tcgcaagtta cgaatacgcc 5700
aattccggca aatggtttgt acttgctgcc gtttatcaag acgtttacga attcggcaca 5760
agctggcgtc gaaagcatga ttcgcaaagc ttgggcgaac cgttggacgg ttgaagaaac 5820
cttaacggcg cttgtcggcg atggaaccgc agcgcagggc acgccgtcgc agttgcaccg 5880
ggtcaacgcg caagccgcgt cggtaatcca tacggcaacc gcgcacgttg cggcggtcgt 5940
tgcggctggc gttatgtctg cggttttcgg tcgttacgtt tggtattcgg taatcgacgg 6000
aagaacgacg gacatttgca taagtcgaaa tcgccgaatc tatcgcttcg gcgaagggcc 6060
gttgccgccc gcgcatatcc gttgccggtc gcacgttgcc ccggcaaata ccgctagtga 6120
cttggccgaa gaaacgtttt atacttgggt tgcccgtcaa cctgcccaag tgcaagacga 6180
catattaggc gacgaaggcg gcgaagcttt gcgcgatggt cgcttaaagg cgaaggatat 6240
tccgaagtat gatgcggaca ggccgctaac ttacgaagaa ttccgacgca agattaaaga 6300
aattctttcc cgctgattcg gtgaatcggc ataaccgcaa ggagtccttg aaatggcact 6360
gaaaaagaaa cttaccaaag aagaacacgc gaagctttcg gatgcactga aagccgaata 6420
tatcgaagac ggcgacggct tccgccttga cgttgacggc gacgaagata cgggcgcttt 6480
gaagcgcgcc aaagaccgcg aagcgcagtt gcgcaaagac gccgaaaagg aagcgaagga 6540
acttcgcgaa cggctggaat ccatcgaagg cgacgacgcg cgcaagaaag gcgacattgc 6600
gacgcttgaa aagtcttggc aatcgaagct tgaaaaacag cgcgaagaat acgaagccaa 6660
ggtttccaag cttaccgcgc atacgacgaa aacgcttgtc gataacgtcg cgtcgcagct 6720
tgcccacaag atttcgaacg ccccggcaat catcatgccg catatcaagt cgcggcttat 6780
cgcagacttc gaaggcgaca cgccggttac gcgcgttctg gacaaggacg gcaagccgtc 6840
ggcccttact atcgacgaac tggcaaacga attcgttgca aacaaggatt tttctgctat 6900
tatcacagct agtaaggcgt ccggcggtgc cggtaagcct tcgcagaacg gcggcggtgc 6960
cccgaaattt aacggtcaat ccgacaaacc cgccgacctt tcgaagatga atcccgcaga 7020
acttgcggcg catcttaaag aagcgaaggc aaacgaaact aaggacgctt aatcatggca 7080
ctttccgacc ttgctgttta ctccgaatac gcttattcgg ctttctccga aaccctgcgc 7140
caacaggttg atttgttcaa cgctgcgacg ggcggggcta tcatgctgca atccgcagcg 7200
caccaaggcg acttttccga cgttgccttt ttcgccaagg taacgggcgg ccttgttcgc 7260
cgtcgtaacg cctacggttc cggcaccgtc gccgaaaagg tcttgaaaca ccttgtcgat 7320
acgtcggtta aggtcgcagc cggtacgccg cctgttcgcc ttgaccccgg ccaattccgt 7380
tggattcagc agaacccgga agtcgcaggc gcggcaatgg ggcagcaact cgccgtcgat 7440
acgatggcgg atatgctgaa cgttggtctt ggttcggtct attcggcact ttcgcaggtt 7500
gcggcggttg tttacgacgc gaccggcaat actgcgcccg acgacggccc gacttggaac 7560
aaccttaaca acggccaagc caagttcggc gaccaatccg cgcagattgc ggcatggatt 7620
atgcacagta cgcccatgca caagctgtac ggtaacaacc tgaacaactc cgaacgcctg 7680
tttacttacg gcaccgtgaa cgttgttcgc gacccgttcg gcaagcttct tgttatgacc 7740
gattcgccaa acctgttcga agccggtacg ccgaacgttt atcgcatcct tggccttgtg 7800
ccgggcgcgg ttatgattgg tcaaaacaac gacttcgacg cgaacgaaga aaccaagaac 7860
ggcgacgaaa acattattcg gacttaccaa gccgaatggt cgtacaacgt cggcgttaag 7920
ggcttcgctt gggacaaggc gaacggcggc aagtcgccga ccgacgcggc gttgttcact 7980
tcgaccaatt gggacaagta cgcaacttcc gaaaaggatt tggcgggcgt tgtcgtcgaa 8040
gttcactaac ggacggcaga cggacggggc ttcggccccg ttcttctagt ccttcacaaa 8100
ttcaaaggag tttcgaagat gaaaccggca aaaatcctgt atttcgtcga tggcaacgca 8160
ccgaccccgg aagacttcgc agccgccgcc gaactgaacg cgcaagttat gttcaggaac 8220
gcccgcgctg ttccgtccga accgcattcg ctggaaatct gcgacggcgt agcgggcaag 8280
gttccgaagc tgtacgccga cgcttacccg gaagcgggcg aagcaatcaa gaagaaggcc 8340
gccgaactga aagcccttgc ttcgaaggtc ggcgacgccc cggcaccgaa ggccagcggc 8400
aaggccgccg acaagcccgc agccgctacg cagggccaga aagccccggc agcggccccg 8460
cagggcggca gcgccccggc ttggaaccct aacccggcgc agtaaccaac ggacggcctg 8520
tcgcttattg tgggcaggcc gtttcaacaa cgaactaagg agtcttcgca atggcaacga 8580
aaaaggttgt ttattttacg gcaggcatta ccgcaaccgc tggcgaactt gccgcaattg 8640
cgaagcttaa cgcagccgcc gaaccgcaat atgaagtttt ggtagccaat ggcgcggcaa 8700
atgccgaata cggcgaaacc gaccgaatca ttccgtcgga ttatgtcgcc ggtactgttc 8760
cttcggaata ttcggcggtt gacgtaatcg accccgacgc aatcccgaat caggcgctta 8820
cgccaacgca agcaatcgtt aacgacgccg aagccttgac cgttccggtt actggcactt 8880
acacgacgac cgcaaccgtt agcgttgcga acggcgttgt aaccggcatc gttctttcgt 8940
aaggacgttg acgcatggca attacaatcg ttgttgaaga cggaagcggc gtaacaaacg 9000
cgaacagtta cgtaagcgtc gcagacgcgc gcatttacgc ttcgaatcgc ggcgtcgaac 9060
ttccgttgga cgacgacgaa ttggcggcca tgctgattcg ttcgacggat tacctagaag 9120
cgcaggcttg ccggttccaa ggcaagccaa cttcgacgac gcaggctttg caatggccgc 9180
gaactggcgt ctttcttaac gaagacgaag taccgtcgaa cgttattccg aagtcgctta 9240
ttgccgcgca agttcagctt gcaatggcga ttaacgcagg cttcgacctt caaccgaacg 9300
tttcgccgca agactacgtt acgcgcgaaa aggtcgggcc gattgaaacg gaatacgccg 9360
acccgctgtc ggttggcatc atgcccacat ttaccgcagc gaacgcactt cttgcgccgc 9420
tgtttggcga atgcgcttcg aacaagtttg cgcttcgtac aataagggtt tgacgaatgg 9480
cacgtttcga cgcagcaatt aaaacggcgc aacgccttat cgcaaagaac ggcgaaaagg 9540
tgaaatggcg cgttatcgaa gacgcgacgc cgacagaccc gaacaagcct tgggaacccg 9600
gcccggcatt gccggacgac aaagacgtta ccatttgctt tttgccggtt gaccggcaga 9660
cgcaagaaac tttcaacttc attaaaggca ccgaagttcc gaaaggttcg gtaatggggc 9720
ttatgggcaa tgttccgttt gaaccgaatt tgaaagacgt tgtaattcgt aacggggtcg 9780
aacttcgctt ggcttatatc gacgtacttt cgccgaacgg gcaaaaggta ctttatacga 9840
tggtatttca agcatgattg aattcgacca agttaacgac gaagtaaacg cgctttttct 9900
tgcaacttgg aacgcaggaa gcgcggcaat tgcgggttat gttcccgaaa ttcgttggca 9960
gggcgtgcaa tatcgcgact tgccggacgg ttcgaagttt tgggttcggc tgtcgaagca 10020
aaccgttttt gaagaacaag cgaccctttc aacctgcgaa ggagtaccgg ggcaaagaaa 10080
atatacggcg tcgggtcttg tcttcgtaca aatcttttgc ccgaaatcga atacgcaagc 10140
gttcgaactt gggcagaaat tggcgaaact ttcccgtaat gcttttcgcg ggaaatcgac 10200
gccgggtaag gtttggtttc gcaatacgcg aattaacgaa cttccgcccg aagaacttta 10260
cgaacggttt aacgtcgtta ccgaatttga atacgacgaa ataggttaag gagttcttaa 10320
catggtatgc gaaattgcca aaatcgacag caacattacc ggacttgcct tcgccgaaga 10380
agaatgcttg aagcaacttc cgacgacgcc cgtttggtac ggactggaac ccaacagcta 10440
ttcggacttc ggcggcgaac tttcgaccgt tgcccgcgcg cctatcgacc cgtcgcgcca 10500
gaacaagaaa ggcacgatta ccgaccttga cgcatcgggc ggcttcaacg ccgactttac 10560
gaagaccaac cttacgcgaa ttctgcaagg cttcttcttc gcagacgcgc gcgaacttcc 10620
ttcgacgcag ccgcttaacg gcgcatcggt cgcacttacc ggcgttaccg cagttgacag 10680
cacttacgcc gcagcttccg ggcttggcgt gttcggtgcc gatatgctgg tatatgcgac 10740
cggctttacc aacgcggcga acaacggcct taaaaccgtc gtttcggcta ccgctgcggg 10800
cgtcgttgtt gctgaaacct tgattgacga aaccccgccc gcaggcgcga agctggaatg 10860
cgtcggtcgc cagcttgccg ccgcagacgc gaacattgcc gtaactggca acgtcgtttc 10920
gcttatcgtt accgctggcg actttacgac catgcccgaa ctgttccccg gtcgttgggt 10980
cttcgtcggc ggcgatgcga cggcaaaccg cttcgctaat aatgtgggct atgcccgcat 11040
taagtcggtt gcagcgaagg cgcttgtttt cgacgacgtg acttggcagg cggccaacga 11100
aaccggaacc ggcaagtcga ttcgtctttt cgtcggaact gttatcaaga acgaaaagac 11160
cccggcactt atcaagcgtc gttcgtatca aatcgaacgc accttgggcg aaggtttgaa 11220
cggtacgcag tgcgaatatc tggaaggcgc agtaccgaac gaatttacgt tgaacgttcc 11280
gcaggccgac aagctgaacg ccgacctttc gtttgttgcg tgcgacaaca cttaccgcag 11340
cggcgacccc ggcgacgaac agaaaaccgg aacccgcgtg cctgcgcccg gcgaagacgc 11400
ttacaacact tcttcggacg tttaccgaat caagatggcc gttcacgacg ccgcgtcgtc 11460
gaaccccgcc gccctgttcg gctacgtttc cgaagcgaac gtttcgatta acaacaacgt 11520
tacgccgaac aaggcggtcg gcgtgttggg cgcgttcgat acttcggcgg gtaacttcga 11580
agtcggcggt tcgattaccg cttactttac gaccgtcgca gcggttaagg ctgttcgcgc 11640
gaacgccgac gttggtttgt cggtaatcag tgcggccaag aacgccggtt tcgtgttcga 11700
tattccgttg cttggtttgg gcggcggtcg tctgaacgtc gaaaaagacg cgccgattac 11760
cgttccgctg gaacccgcag gcgcagaaaa cgcgaacggc tatacgatgc tgtacgaagt 11820
gttttcttat ctgccgaatc tggcaatgcc ggactaattg cggtaaactc aaagggccgg 11880
gtaataccgg ccctttcttc attcaatcgg agtaaatcaa atgtctggac tgtttaagca 11940
attcaaaacg aattcgacca aggaagtcga aggcgtcgaa atcgaatttc ccgaagcgca 12000
gaacgacgac ggcagcgttc cgactttcgt tatttcccgc atgggcaaat cgaacaaggc 12060
gtattcaaag gcgcttgacg cggcgacccg tccttatcgc cgtcaagtcg aacttggcac 12120
gctgaaaaac gaagtcgccg aatcgctgtt tatgggcgtg ttcgtcgata ccgtgttgcg 12180
cggctggaaa aacgttcagg gcgaagacgg caaggaaatt gcgtattcga aagacgccgc 12240
aatttcgctt ctttccgaac tgccggacgt ttacgaacgt ttgcaggaag aagccaagtt 12300
ggcttcgaac ttccgcgata acgctttgga agccgaagca aaaaactaac cgaagttttg 12360
gcgcacctgt tggaacttgg cccacacgaa caggcgatag caaaacaagc gatgcgcgcg 12420
gggcagccgt tacccgaacg catcgcgaac gcgccagaac ttgaagtagg cttgcagttg 12480
tatttgcaag ccttcttcga cctagatagc gaacggtcgc acggcaacgg tttaacgccg 12540
ataccttgga caagtatggc ggcttacgca agggctttcg agtttgacga agaacaaacc 12600
gaagacttgt tttactttat gcgaaagctt gattcggaac acttgaagaa actagcggat 12660
aaacagaaag cggcggcatc gaatggcaaa aagcctgcta gacctagccg ataggctgga 12720
aaagaaagcg aaggcaatag acgaagcggc gtcacaaaac gccgtcgata ccgctttggc 12780
tattgtgggc gacttggcgt acaaaacgcc cgtagatact tcgcaagctt tgtcgaactg 12840
gattgtaacg ctagaaagtc cgtcgggtca acaaatcaag ccgcattttc cgggttcgca 12900
gggttcaacg caacgcgctt cggcggctga aacgttgaat tctgcgaagc ttgttttacg 12960
aaacaagaag cccggccaag cgatatttat tacgaacaac cttccgtata tccgcaggct 13020
gaacgacggt tattcggcac aagccccggc ggggttcgtc gaacgcgccg ttttgattgg 13080
tcgcaaaatg cgtaagaagt tcaagattaa ggattaaaag aaatggccga cgaaaacata 13140
gaaattaagg ttcaagacaa agtttcgccg tccatttcga cgaagcttcg cacaattgca 13200
agcgaagccc gcaacgccga cgccgcagtt aagaaccttc aaacgcagct ttcggcaatt 13260
aacgtcggcg gcttgtcgca gcttatcaac gcatcggcca gcgcaacgcg ccagcttcaa 13320
caaggcgcgc ttgccgcgca acggctggca accgaacagc agcgcaccgc aacagccgca 13380
gcgcaggccg ccgcagccca acagcgcgtc gcgacggctg cgacccaagg ggcaaccgcg 13440
caagccaacc ttgccagcgc cacgcagcgc acgcagacgg cccaacagca gacggcgaca 13500
gccgcgcagc gcctagcgac agaacagcag cggacagccg ttcagacggc caacgcggcg 13560
gcagcgaacg accgggccgc ccttgccgcc ctgcggctgc aacaggcgca agaccgggcc 13620
gccaattcga cccgcaatgc gacttcggcc cttggcggat acattcgaac cgctgccgga 13680
attcttggcg taacgatttc ggcgaacgca attcttgcaa gcgccgacgc atacgttacg 13740
cttcaaaaca agttgcagaa cgtaacgaag tcgcaagaac aagttaacac gcttacgaac 13800
gaacttttcg aactgtcgaa ccgcacgcgc gcgggtattg aagaaacggc gacagcgttt 13860
acacgcttcg accgtgcctt ggcgtttatg ggtaagtcgc aagaagattc gttgcgaatg 13920
accgaaacca ttaacaaggc tttgattgtt tccggtgcaa cagcgcaaga agcgtcgtcg 13980
gctttgttgc agctttcgca gggcttcaac gccggtaagc tgcaaggcga cgaattccgc 14040
gccgtttcgg aaaatatgcc aatcgttttg gacgcagtag cgaaggcgct taacgttccg 14100
attaaccgcg ttaaagaact ttcgaccgaa ggtaagatta cttccgaagt tctgtttgaa 14160
gctttcaagc ttatacaaga ccaagtagac gcaacgttta acaagacgac gcccacaatc 14220
ggccaaagct tgacggttct tcgcaacaat gcaattcagt tcttcggcga attgaacaaa 14280
gcgaccggct ttactgccgg gctttcgcgc gcgattattt ggcttggcga aaacttgaag 14340
acggttgccg taattgtcgc cgggcttggc gtcgcattgc ttgtcgcctt cggtgccccg 14400
ttggttggtg cgctggccgg tgcgacggct gcggtaaaag cctttacgct ggcgcttgct 14460
tcgaacccga ttggccttat cgtcgtcgcg ctttcgaccg cgattgcgta tctaacgctt 14520
ttccgcgacg aaattaactt gggaatcgac gacgttacga cgttgggcga tttcttccgc 14580
gcgacctttg aaggcatcgg ccaagcaatc gacgacgtta aaaccatcgc gtcgcaattg 14640
tgggcagaac ttagcgactt cgcaagcgca gcccttggcg aaattacttc gaacgttgac 14700
gattcgactt cttcttggtc ggacagttac gccgaatttt tccaaaccaa ccgtacaggt 14760
tgggccggtg cgttggaaaa cgccgcaaaa gttcttgacg caatcgccgg gcttttgacc 14820
ggggccgcaa cgttcgcagg ccgtgcaatg gccgaagttg ttatttcggt tcaaaacgga 14880
atcgcaaacg cttacaacgt tgtcgcgggt tggattgaaa acgtaacgaa taaagccatt 14940
gaagccgcga acaggcttcg cgcgatggtt ggtaagtcgg cttacgaact tgtccaattc 15000
gaacgcatgg gcagcgcagg ccaaaccgaa ttcgaatctt ggggcaagct ttgggcgcaa 15060
tcgctggaag acggatttaa cagccaaggc ggcgcaatgc aaaacctgtt gaacggcctt 15120
ttcgaccgtt cgcagcaaat cggcgcgcag cgcagggccg caggaagcgc gcagcttcgc 15180
ggggccggta cgtcgcagct tgccggggct accgacgcaa acgcggcgaa ggctgcggaa 15240
cgtcgcgcgc tggcgttgga aaaaatcaat acgcagcttg ataacgaatt ggcgcgcatg 15300
ttccagcttc aaccgcagcg cgaagcgcaa gcgaagttcg accaaatcga agaaagcttg 15360
attcagaaaa agattaagct tacgtcggaa gaagccgaag caatcaaggc gaagattaag 15420
gccgttcaag acgcgaccga agttcaacgc cagttcgacg caatttacgc cgaagctgtt 15480
aaccctttga aggagtacaa cgcggcgcag gaagcggcga acaagcttct tcaaatgggc 15540
gcgattacgc aggaacagca cgcccgcgca gtaacgaagg cgtcggaagc ttacgcgaat 15600
tcgcaagacc cgttgcggca gtataaccgc gaccttgaac agcaattgca gcttttgcaa 15660
atgctgccga agcaacgcga aatcgaacag caggttatgc aagttcagaa cgatttgctt 15720
gcaaagggca tcgttctaaa cgaaaccgaa ttggcgcagc ttcgcgaaaa gctgttgctt 15780
attcagcaag taaacgccgt ttcgcagcaa gaagcgtcgt tgttggacgc cagcgtaaac 15840
aagcgccaac agtttatcga ccaacttaaa gcaattcaga acttgcgcaa caacagcgga 15900
agcggtttca acgcaggcga ccaagccgaa gcaactaaca gcatgttgca aggcatgggc 15960
attgatacga cgaactttca aacgcagctt aacgcgcaat tggcgcaata tcaaacttac 16020
gtcgaacagc ttaaaatgct gaacgaacag cgtttgatta gcgaccaaga atacgccgcc 16080
gcaagtatgc aacttgaatt gcagcggcaa aacctgtact tgaattcggc aagtagtttc 16140
ttcggaaact tggccgcgct tcaacagtcg ggaaataaga aaatggcggc ggtcgggaag 16200
gccgcagcaa tcgcgcaagc gatgattaat acgtatcaat cggcaacgtc ggcttatgct 16260
gcgatggcgt cgattcctta cgtcggcccg gcgttgggcg cggctgcggc tgcggctgca 16320
attgccgcag gcttggcgaa cgttcagcaa atccggtcgc agaataccgg gtttaagtcg 16380
ggcggtttta ccgggtcaat gggcgtaaac gaagtcgcgg gcgtcgtaca cggccaagaa 16440
ttcgttatga acgcagcggc gaccaaccgt attggcgttg cagaccttca agccctgcaa 16500
acgggcgcgg ccagtgttca gcgcaacgac gaacaggcaa tggcggctag tgtgggcagg 16560
ggcggcaatt ccgaacccgc gcccgcgccg gttgtcaatg taccttttag cgccgttgtc 16620
gtacaatcga aagaagcggc cttggccggg cttaaatctg cggaaggtcg ggctttcatt 16680
ctggaaacta tcgaacaaaa cggcggcacc gtcgcaaaaa tcgtcggagt taaataaatg 16740
ggttacgcaa ttggaacagt tacaaaaggc ggcggcgacg attgccatta tcaagttttg 16800
gcaattatca aaacgctggc cgaagcgaac ggatggacga cgcttcgtta cgtatcaacc 16860
ggacttgacc gcgaattgat tttgaaaggc gttggtttgt ccggtcttga agaaatcttt 16920
gtagggttta agtgttatca aagcgtcggc ggcgattatt acaatattaa ttgcggaact 16980
ttcgtcggtt acgtaagcgg aaacactttc gaaacgcaac ccggcgcgca gtttagcgca 17040
acgcctgccc acaataacgc gattacgtat ttcataacgg ccaacgccca acgaatcgtc 17100
ggatgcttca aagtcggtac gccggtttac gaacattttt acgtcggcaa gttctttccg 17160
tatgcgcggc ccggtgaata tccgtcgccg cttgtttgcg gtgcgatgct ttccaacgcg 17220
caggcaatgc gattcgacaa caacgcgcag tcgttcccgt atccgggcta ttattacgac 17280
acgacgaaca accgccttta tattcgcgac caagcgggca actggattcg cccttggtgt 17340
tggccattta ccaatggaaa cggcgcaaac accgcgcttg cagggccgca aggaacttcg 17400
acgcttgttc ctgccgacgt gtattaccaa cttgaaccaa ttattttggc gcagcaaagc 17460
aacaataaca acccttcgaa cgtatggggc gaattcgacg gcgtttattt ctgttccggc 17520
tttaacaacg gcgtcgaaaa cgtagttcag gaaggcggaa gttctgtcgt tgaccaaacc 17580
gggcttaccg tacttcaagc cgtagacgct attaaggcgg tcggcgggcg ggcgtttgta 17640
atgttgcaaa acgtctatcg tacttcttgg cgcgatttta tcgctttgga aatgaaataa 17700
tggcatacat tacaggaacc gccgcaaact ttgcggattt gaaaacagcc attgaaaacg 17760
cctgtacttc taacggttgg gcgcttgccg acggcatatt gtcgaagaac ggttgtttct 17820
ttcaactaat cgccgatagt ttgggcggtt atccgcaact tcgcttgcat ggcggaacag 17880
gcaaaagcgg ttcgacgctt acaggccagc cgcctagcaa ttacggggca aaggtcgcaa 17940
gccctgtcgg aaatcaaatc gtattcccga ttaattacga aatacatttg tttaccgacc 18000
ccgacgaagt ttattgtatc attaattaca attcggactt ttaccaacaa ctttcgttcg 18060
gcaagtctga tattccgggc attggtggaa ctggcgcatg gtttaccggg gcatatcggc 18120
acgactacgg attgaccgac gcaacttacg cccatagggt ttttacggcg atttcttggc 18180
ccgaaataac tggcgtcgca acttatgccg gaatgtgcgt cggtttgttt tttgaaggca 18240
acgcttcgca aactggcgga agttatttcg caagcttcgt acataccggg cttgattcga 18300
cgccttggcg aagcggccac ggcggcgaca cgctgttaag cggctatcgc ggggccggtc 18360
tttccgtcgg ttcgctttta atgtcactgc cgaatatttc gaaccaagca acgattttgc 18420
ttcctattaa gccggttgcg cgtcgttcaa gcggcggcgc aactattatc gcaaacccga 18480
aaaacgcgcg ttatttgcga atcgacaacg ttgtaccggg cgaaattatt acattcggtg 18540
ccgaccaatg gaaggtttac ccgttttacc gcaaagacgc aaccgttcga aacggcgtcg 18600
gttggtcaag cggcgcgacg catagcggaa cgttcggata cgccattaaa tacactggaa 18660
cttaaaaaga atggccggaa gaattggaac atttttagcg gcggaaagtt ttatttacgg 18720
cgattataac ccgaaccttt ccgccgaact taacgaactt accgccgcag aatacgcgcc 18780
gacgatttac gacccgcgcg atatgttctt gcgcggcgga tattcggcgt acaaaatcgc 18840
gccttcgaat cgctggccta ttgtgggcaa agaacaaggc aacttcgggc gcgactatta 18900
ttatcgcgtt cacgtttcgc cattggtctt ggacttgcaa acggtcgcgt cgtcgcaaac 18960
gcgcgaattc aacgtttgga acgcatggcc cgaccgtgcg gctaatcttg acgacatttt 19020
ggtaagtaat ccggtcggta ttgaaattac aggccaagcg acgccgtatg caatgccgcc 19080
gcttcaagaa cttacttacg aaataaccgt aggcgtttcg gggccgccga atattaacgt 19140
tgaagttcaa tttgactttt ccaacgttcc cgacccgctg ccgattctga ttacaggaac 19200
gcgcgcggtt aagttcgaca ttgtgcccga agttccattg aacgaaactt gggaatggtt 19260
aagcgacaac attgtagcgg ttgacggaac agaacagcgc atagcacttc gcggcgaaat 19320
gccgcgtatt gaagaacgct tgaaagtaat ttttgattct gtcgaagccg tgcgccgttt 19380
ttacggcgac gttatgtcgg cagttggtcg gctttggatt cccgaatttc agtacgcgac 19440
ccgaattact gccgcaagca atagcggcgc gcttcaaatt tattttgacc gaacgaagac 19500
cgacgttcgc gataccgaat acgtcttgat tcaaacgccg cttgttgcgg cacttgtcga 19560
aatcgacgta ttgacagccg acggggctac gcttggttcg ccgctgttgt tcgacattcc 19620
ggcagggtcg ttaatcatgc cgggttcgcc cgcgcttatt gacgacaata cagcacttgc 19680
gcggtattcc gtcgataccg tcggcgaagt agaaattaac gcgaagatga ttcgccagcg 19740
ttcgcaactt acccgacccg gcgcaaccgt cgtacttgaa cagttcggcg gcgtgccgat 19800
tttgacgaag cgtccgcttg cgaacgacct tgtacgcgac gaagtttcaa ccggccaaat 19860
ttcaatcgac aatcaaaccg gacttgccga cattattagc cgttgggact atagccgcat 19920
cggcgggccg cgaagcttca aagtaaaccg tatttcgaat cccgaagaat tggattattg 19980
gaaagcgatt cttgcgtatg cacgcggaca ggcgcgcaag ttttggatgc ccacatatcg 20040
aaccgatttg gctttggccg tgcaaccgtc cgacgcgact tcaagctata ccgtcgaagg 20100
gtcggagtat gccgaaaaga tttggccgat tatcacgcat cgttatatcg aaatcgaaac 20160
agcgtccggc attcatcgaa ccgaaatatt gggcgcaact ttgcttgaaa ctggcaatac 20220
aattcttctt cttggtacgc cgcttccgac cggggccgga tggatggaag ttaagcgaat 20280
ttcgtacttg ttgcctgtac gccttggcga cgataaagta gaatggaagc attacggcct 20340
tgaatcaatc ttgaatcttt caattcgaac ggcggaaccc taatgtctga ttacgacgac 20400
aaagaagaaa gcgtaggcga tagcgcgcca tttgaacttt acgaattcgt cggttcatat 20460
cgcaattatt acatgacttc cgacgttctg gcgcacgtct tcaacagtgc gacatataac 20520
cccgttccgg gtttgaagcg cagtacgtta aaagtcggaa cgcacgaaga cgacaacgta 20580
gatatgacgg ttacgcttcc gattacggaa caaatcgtta aagactatgc gtttcaaact 20640
acgccgccgt ctttggtttt gacgatttac cgtcgccagc gcgacgccgc aacttgggtt 20700
gcatactgga aagggccgat tgcttcaatt tcggttgacg acgaatttgt aacgtttaga 20760
acgccaagca agttcggttc gatgctgtcg ggcaatatac cgaacgttta cgtacagccg 20820
ccttgcaata acgttttgtt cgacgaacgt tgtaaggtaa gtcgcgtaag caattcgcta 20880
gatacgcagg tttcggcggt cgaaggtcgt aacatttcga ttccgtcgct tggcggtttt 20940
cccgacggtt ggtttatcgg cggcgaaatc gcaatacctg cgcgcaacga acgtcgaatg 21000
attgtcgcgc aagccggggc aattctaacg gtaaattacg aattcgcgcg tatttctgtc 21060
ggcacttcgg ttcaggttac ggcaggttgc gaccattctt acaacggggc caacggttgc 21120
cccaagttca acaatcaacc gaacttcggc ggttgcccgt ttgttccggg cgaatcgaat 21180
aacgtatttc aaagcggggt tcattaacaa atgtggattg caattgtctt cgtcgtcgcc 21240
ttcttactta tggcggcgct tatgccgaaa ccgaacgtcg aaaacgcacg cgcggcgaaa 21300
cttggcgact tccaatttcc gcgttctaag tacggcgacc caatgccgct agtgtgggga 21360
acagtgcgcc agaaatcgcc gataacggct tggtttggcg acttccgccc ggtgccaatt 21420
cgtaagaaaa tgaagaccgg gctttttagt tcgaagaaag ttacggtcgg ttataaaaat 21480
tatcttggca ttgattgcat tttgtgcctt ggccccggcg ttcggcttcg taagttttgg 21540
gccggtactt accttgtttg ggaaggcaac ttaacttcgt ccggcgatat ttacattaac 21600
aagccgaact tgtttggcgg cgaagatgaa cgcggcggcc tgcaaggtac aatttcgttt 21660
tacgacggtc gttttgaccc gccgcaagat tcttacttgg tttcgaagat tggcccgaat 21720
gttccggctt acaacggctt tgcgcgtgcg ttgttcaaag ccttttatat cggaacaggt 21780
acgacgccgg aaccgttcag cttcgaaatt cagcgcatta cgtccggctt gcacgcgact 21840
tattcaatca tgccaaacgg gttggacgtt aacccgatgg aaattgttta cgacgcaatg 21900
acgcaaaaat ggggccgatt cggcaacctt gaaagcgaaa ttgatatgcc ttcgttcacg 21960
gcctgcgcgc aaacgctgta taacgaaggc ttgggtatgt cgcttattgt tcaatctgcg 22020
attaccggaa aagacttgtt ggaagaagta atgcgcgtcg ccgacggcgt gttgtatcaa 22080
gacccggcaa cggcgaagat tgttgcgaaa cttgttcgcc aagattacac aatttccgaa 22140
cttcttgttc ttgacgaatc ttccgttaag gaacttcgca atttccagaa aacaacatgg 22200
gaaaacacct ttaaccaatg ccgggttacg ttcaaaaacc gcgccagtag ttacgacgac 22260
agcgtagcaa ttacccaaga cttcgcaaat atcaactttc aaaaccgcgt taagtctacg 22320
gaagtttcga cgcccggcgt taccgacgca acggtagcaa gcaagattgc ggcgcgtcaa 22380
ctttcaattt tgaacgttcc gctttacaag tgcgacttga ccgttaaccg caaggcgcag 22440
aacttgcggc ccggaagcgt gttcgttctg aattggtcgc cgttcggcat ttcgaatatg 22500
gttatgcgtg ttacgaaaat tgatttcggc gaacttacgt ctaaccaaat caagctttct 22560
tgcgttcaag accgcttttc gtcttcaacg cttacgttcg caccgcccga aggttcagga 22620
tggacgccaa ttagtacgga accgacgccg gttacaacgc ggcttctttt tacgccgcct 22680
gcgttcctta cgtcaaacag cgaaaacgaa acactttcca attttgataa ctacggtcgg 22740
ttgtatcttg ccgcagttgc gccgggttct acgtctattt cttacgacgc aatgacggcc 22800
ccggacaatt tcagttcgtc gcctattctg tcgcttgaaa gtgccccgta caacggcggc 22860
ggcgtgttgc tgaacgccta cgcttcgaca gtcgccagcg aaacccgtaa cgatacaagc 22920
ggaagccttg tcgtaaccgg cgtgtcgcaa gcaagcattg aaggtttgca gcaatacacg 22980
acgctagacc aagcgcgcga cggttcggcc ttcctgctga ttaataacga actgttcgtt 23040
tatgtgggct tcgttgacaa tggcggcggt caagttactt tcccgaacgt gtatcgcggc 23100
gttctggata ccgtgccggg caatcatgcg gcgaacgacc gcgtttggtt cattagcgga 23160
accgacggac tgttgccgga acttgtttcg gtcggaacga cgcgatacgt taagcttttg 23220
gatacgactt cgggcggcaa gcttgatatt tcgttggccc cgtcgtttag cgggtcgctt 23280
accaaccggg ccgggttgcc gctgccgccg cagtacctaa cgcttgcagg aagccgcacg 23340
ccaagcccac aagtaggcgc aacgtcgatt gttgcggaat ggcgcaaccg cagccgggcc 23400
gataccttgt tgcgcgtgta tcacgacacg gcgaacacgc gggaagctgg caccgaaacc 23460
cgcgttcgct ggcgtgtagg cggcggcgct tacacgaccg ttacgactgc cggaagttca 23520
acaacccttg acgtaacggg caagtacggc acgctagaag ttattgtaga ttcgttcatt 23580
acgtcgaatt cgaaatattc gacgtacagc gattcgctta caatgaccct aacggcaccg 23640
ccgccgccgt aatcaaaaga acgacagcgc ggcggttttg gccttcttca accgcccgca 23700
gtcgtacaac atttcgaccg cttcgttaat gtaccaatcg taatttatat cgtcgggaaa 23760
ttccgtcggc aagtccatta cagggcgcgc gccgtcggtt ttcccgactt tgtttccgct 23820
ggatacgtaa gcaatatatc cggcttcgtt cttcgggtaa taccaacgaa cgaccttgcc 23880
aagataacga ccgttctttt cgccgccgcc gcgcacgttc tttactgcaa cgaagcgccg 23940
gaagtcgcgg cattccttaa ttgtcttttc gaccggcgtt ccgtcaacaa ggaaccgaac 24000
aacggcgtcg gaacaaatca gggtttcggg gttcttcgaa agaatcgaat tcaaagccga 24060
cccgcgttcg caataagcgc ccttcgtttt tgcgcccaac tgttcgtcaa taaagcgcgc 24120
ttcggggtcg ccgccgtctt ccttgattgc gacataacta ttaacgtcgc ggctgaatac 24180
ggctttatat cgcgtttctt cggtcttgta tcctgtccat tcttcccaag acgcaataag 24240
cgcccttact tcgtcgtgtc gcgatttgtg atacttggaa ataatgccgt cggtattgcc 24300
ggaaataact tcgatgccga tttgttccaa agcttcgata agcataagca acacaagttg 24360
cccggtaatc gttacttgca acataagttg cggcgcgtaa agcgtcgaat acttgttgcc 24420
aagcttgccg aagcttccgt taatggtaat tttcaaactg tcggcaattg tcttccattt 24480
cttcgcggct gcgcggtcgc ccgccttctt tgcctttgcc gcttcggctt tggcgtgaat 24540
tcgcgtattt acgatttttt cgtaaacggt caagaaagct tcgcccaagt gcggcgggca 24600
aagtctttga ttaaggattg ttcgcgggta aaacgattct acgtcgttgt ctgccaaaat 24660
tatttcgtcg gtcgcatagt gcgccgtttt cttttcggta gaatgcaaac cgcccatgcc 24720
tagtttgtac gtcgaaccgc caatggttac tttcagcttt tccagttcgg gcggcatgat 24780
tggcgacccc aagccgtcaa gatagaagcg ggcatttcgc acgacttcca gcatttcgcg 24840
aagctgcgtc gtttgatagc aaacgaagtc gggcacgtcg taaaccaaga ccgtatcggc 24900
ggatagtgtg ggcttcttcg gataatagcc aaggactttt tgaagttcgg agttaataac 24960
cgcttcggct acttgtgcgt ctgatttcga ccgaaggtct acgccgtatt cttccgacat 25020
ttcggcccgc aacttcaatt cgggcgcaag ttcgttaaaa agaagttcgg tattcgaaag 25080
gtcgttgcag caataaggcc gcactatttc ggcgtcttcc tgcgttaata catggtcttc 25140
gggaaacggc aagtcttgca tacgttcgca atgcaagcgc cccgcgtaca gcttcaacga 25200
accttgaagc ggcgcaactt ctataaggtc gatatgattg tatcggccaa tttgtacgcc 25260
gtacttcttt tcgaacgcga acggcgttac cttttcggtt ccatagttcg gcccgctttt 25320
aatgatgaaa tcggacgctt ctttaagctt cgcgcagctt gccccgcgtg cggctagttc 25380
aatcatcggc aagtcataat tacgcgaatt gaagccgacg atacagaacc gccaaagcat 25440
ccaaagaagc ttcgtcgggt taaaatcatg gtcggggctg cgttcgaacg cgacgaactt 25500
tccgttatca agcgacttaa acgctacgta aaagaaattt acgtaagttt caacgtcgaa 25560
gacgaagaca cttccggcag gcattgccgt tagttcttcg tccgacataa attcgacagg 25620
ccgcaaagcc tgtcgaatgc ttgcggccag cttatcgacg acgcggcttt tctttgcgac 25680
gataaagcct tgttcgttaa tcatgcgaaa gccccttaaa acggtatgtc gtcttcgtaa 25740
ttgtccgacg gattatgcgt cgcttcgctg ccaaggtcaa gacccataag aacgccgcgc 25800
aaattgtcgc cgaaaaatac gaccttgttt gcttccggtg cgaaatgcgc tttcttaaac 25860
gcatgttcga cggaaagaag caacttcgca gaaaagccca ttcgttccgg caagccttcg 25920
attttgtacg tcgaagcttc ttctttgtgc atcttcgaca aaaccgcgcc gtcgtcgaag 25980
aatacgtttc cgttcgggct gaatgactga atcgcgcgaa cgccgttgta aaaatcgtcg 26040
ggaaccggcc aagggttcaa accgtcaact tcgaaaacga cttggtaatt cgggtaacgt 26100
tcgccgtaaa gctgcgtttt gataaacgcc ccgttttcga aatagaacgt tgccgacgac 26160
gccgaaaagc cgaagccggt aagggccggg ccagccttgg cgacagcgac cgccgcgcac 26220
ttcggaagca tcatgccggg cggaaggtcg ataccgtgcc acgcttccag caaggccgcc 26280
ccgttggtcg cgacggcgct tccggcttgc agcaagacgg cggcataggt cgcattcggt 26340
gcgccgtctg tcgcaagccc tgcgaccgca gcgaaggccg ttttaatccg gtcgtcgatt 26400
gtggcgcatt gcgggtcggg cgcggtaatc ggcacgtcgt cgaagcccac acaaggcacc 26460
aacgcccgga acgcgccaga cgatacggca agggcgttcg cggtaagctg cgtaatcgac 26520
agttcttcgc cgaccttcga cagcgcgtcg ataaactgca acgtatgcgg gcacgctacc 26580
aagtcttctt cgataggatg cgcgacggtc aaaaccccgt cggaagccgc cgcccaattg 26640
tgggcaatat gcccgaactg aacgttcgtc ggcccggctt ttttctgcgc gatggcaacg 26700
aacttcaacg ccgccaaaag cgacgccgca gggcttggcc ccttcgcctt tttctttccg 26760
gctgcggctt gcctgcgcgc ccttggcttt ttcttcggct gttcggcggc tggcgcttcg 26820
tcttcgggcg gaagcggaat attactgtcg attttcattt tcggcccctt cttcgtcaag 26880
tttcgaaatt gccgcttgaa gatagttcgc taagtctagc gtttcttcta gcgcgtgttg 26940
caaccaatcg cgaagcgata acgggttatt atccgtcgtt acgccgtact ttgcaagtcc 27000
aacgattgaa cgttgcaaca gtaattcgcg gttcttttct acgtttttat cgggcgatgt 27060
ttcggccatt tgttcaccat tcagcggaaa gaacttcggg atactttttg ttcacccata 27120
cgcgaacacg gcgcggcgtg cgaagttctg aaacacgctg caaagcttga taagtcgtcg 27180
gcggcggttc ttcggcgtgc cgctgccgcc accaatcgcg cgaacgtttc gcagcaagcc 27240
ccggatgttc aagcattata aattcgttga acatttggaa gccgcaaaag taactaacct 27300
taatcatcgg cggtttcgtt agatttccgt ttgcgtcgcg cttttcgtgc aaattgtaaa 27360
gtaccttttg aacgtcgaaa tattcgacta tcggcgcgtc gcttcgcagc ggttccagcg 27420
ttccggcagt tgcaaacaac ttcgtttcaa aagtaaattc ggtgccgcag ttgatacact 27480
gccgggccgc agcgtgatta tatacgccgc atacgtcgca aatacgaacg ggcgcgtcgc 27540
cgccgccttt gcccggtctg ttcggaattc gcgggtcgtt gattgggcca agccttcgaa 27600
cgtttccggc aaagtccgcg tacaggcaat tttgtttgcc agtatccggc gacggtcggg 27660
ttccgcgtcc caacttttga acgtgtttgc ccggcgatag cgtcgggttc aaatcgccga 27720
taaagtcgat tggcggatgg tcgaaacccg ttgtaagttt ctgccctgat accaagccgc 27780
gaagttcgcc agctttgaaa gcgcgcaagc gttcggtatt ggtcttcgaa ggcaacttgg 27840
aatgaaccgg caaaacttcc aagccgtaag actgaattac gttcgcgacg tgttcggtat 27900
tatcaatgcc agttgcgaag ataagccaag tcgaacggtc gtaagctatt tcggtcattt 27960
cccgaacagc gttaaaaaca acttcgtcgg tatctacggc ggcttctagt tgcttgctgt 28020
tgaattcgcc agccgttacg cctactttcg acaagtcgat ttcggttcgc gtcggccttg 28080
cgataagcgg cgacaagtag ccttcggcga taagtcggtt aaacgattcg acgccggtta 28140
tatcgtaaca aatgtcggta aaaattccgt tgtcggtaat aagccccatt ttcattcgat 28200
agggcgttgc cgtaaatcca ataactttaa gatgcgggtt aatcttgcga agttcggcga 28260
taatgtattg gtaatgcgaa tcttccttgt cgcttaacaa atggcattcg tcgattaaca 28320
gcaagtcgcg ccaaccgaaa tggcgaagat gcgccgggcg tccgtcgtct tcttcaagcc 28380
cgcgtttaat cgcaggcgct acggactgaa cgccgccgaa tacgattggc ataatcatat 28440
cgcgggaatt caagcccgcc gaataaatgc ccattggcgc ggtcggccaa atcgacataa 28500
gcttttcggc gttctgttcg attagttctt taacgtgcgt aagcatcatt attcgttgat 28560
tcggccagta accgaaaatt cgacgaataa agtttgcaat tacgaccgac tttccggttc 28620
ctgtcggcat ggcgacgacg gggtttccga ccccgccgcg ctggaaataa tcgaatattg 28680
aatattccgc ttcgtcttga taccaacgcg gaacataaat attagacatt ctttgcaacc 28740
gctgtaattg gcgaataaga atcgcaaccc gtagcgatga aatcgcccgg aattacgccg 28800
ttgtgaactt cgcaaaacca ttcgccgttt tcgacaggat ttgcggcctt gcagcttcgg 28860
cagttctttt caggaatcgc gcccttgtgg caaatatcct tcgccgcgca atatccgcac 28920
ttgtgaaacg tcggattatc cgacaagcgc ggcggcgctt cctgcgacag aatgatttgt 28980
tcggccttct ccttcatttg ctggcctagc cgatggtcta gctttacaac ttcgcaatgc 29040
aagtcgtcgt cgttcttgtt aatgttcagg taaagaacat aacgaaaacg atattcgttg 29100
ccatacgtcg aagtttgggc gaaatgctgc ggcttgctta ctgccattcc ttgcgacatt 29160
aagtccgaaa agcctttgcc ggttccgttg gtcttgaatt ccagcaaaac gggttcgtcg 29220
ataccgtagc gttcgggaag ccgggcgatg ccgtcaagcg acccgccgaa gtgccccata 29280
acgccggaaa tgcggtattg tgggaattcc aagccgtcgg ctttcgcgcg tgcgacgtgt 29340
tgggcgtaat acggattttc gttcgtaata aggtcgcagc ccaacggcag ttcttcgcct 29400
tccttcaata tgccgtaact gtcgctttcg gcttggtaaa agaaaccgtc gcggttttcg 29460
taccaacatt gaacgccgat gccttccagc cattcgataa agcgggcttc ttcacggtgc 29520
ccacgattaa acaagcgttg cttgcgcccg tcggtttgtt cgcggaaaca ccaacggaag 29580
atataccaaa gcttccgctt gcattcgtcg ccaatcaaag acgcgccaag gtgccgacgg 29640
tgcccgccgt catacgtgcg aacgcaatat tcgtctatat cttctttaat gcgcttcgca 29700
agggcggttg caacgccggg cgcgtcaagg tctacgcttt gttttcctgc gtcggatttc 29760
gtcggctgcg cgtcgttcag cgttaattgt gcgttcttcg cgtctttgct gttttgcatc 29820
gttcttcacc ttgcgccgca taagggcttc gtaattgttc gctagataat ccgccgaaag 29880
tgtcgcaacg tctttaagtt ggtctttcga cagccaagat atatggcatt ccgacgggtc 29940
gattcctaat tggttcgcaa gccaattgta cgccttcgcg cgcgacatta agccggtttg 30000
ccaaaggcgg tcgaattcgt tatgggcttt cgttcgaagc tggcgcgttg ttctgtcggc 30060
catacgaccc aacggaataa acgtgcccgg atggcaacca acggcggcgc gacaatcatt 30120
gcaataataa atgtgcggcc aattgccata agttcgcccg taaatcttgt cgttcgttgt 30180
tagttcaatg ttgaacgaac aacaagtatc gcattgttcg ggcgtcggca atgcgtcttt 30240
tattttcgac ataagttcgc atactcctta aaaagaaacg ccggggccgg ttaagacccc 30300
ggcgaatcta cgccgcgacg gcgttacgcc ttaacgctgg ccccaaggtg ccgcgcctgc 30360
cggtgcgccg ccctgcgcgg gctggccgcc gccctgttgc cagccgccgc cctgcggttg 30420
ctgctgcgcg ggctgttggg caggctggcc gccccatgcc ggttggccgc cctgcggggc 30480
cgggttctgc gccggttggc cgccccaagc tgcgccgccc tgcggctgtt gggccggttg 30540
ctgcgcgggc tgctgctgtc cgccccaagc ggcaccgcct gcgggctgct gcggctggcc 30600
ctgcggggca ccgccccaag cgccgttcgg ctggccctgc ggctgctgtt gctgcggctg 30660
cgctgccgct gcgcctgcgc cagccttgcc cggttcgttc ccgttcatat cgaagacctt 30720
tttaacttcg gtatactgcg ggtcgttttt ctgcgggccg acttcgataa gaaacggcag 30780
gttgtgaagc tgcgacgaat cggtaacttg gaacacgccg attacgtggc agattgccga 30840
aagctggcga tgcgcgattt cgacggtctg ttggttggaa tggtacaggt tcaggcgata 30900
agcgccggtc gtgccctgct gcgggccgtc gataatccgc aagttcagtt gcagataacc 30960
gccgtcgttc gccttgttgg ccttgacttc ggaagattcg acgataaccg gatgcttgcc 31020
aatcggcagg cttccgacgc cttgggtcgg gtcgtactgt tgggcgttga acgcttggat 31080
aagttgcatg atgtttcacc ttttcgaaaa gtcttcgcca ttggttacgg cggcgaagta 31140
aaccgtttta ttgcatcgct ttcgcgaaca atgcggtaag gtcgggctgt tcaagttcgt 31200
taagattgcc caaacggtcg cgcgcgaaaa cttcgggaat ttccttcgtt cgcagcgccc 31260
gaaccggctt cggcattccg ggtacgcttg cttcgcccaa gtgcataacg ttatcgaaca 31320
aatgcggaac tttaacgtta aggtctttgc ccggaaagaa cgggcgtttt tgcataatcg 31380
gttcgtaagt aacttcgccg ttctgcaaaa tcgtttgccg tccgttttcg acaagggctt 31440
gctttgcaat cattacgata tgcttttgcg gcatgtaata caagtcgttc gcgattttca 31500
ttacacgttc ggacatattg ccgtaagcct tcataccgtg tttaaccttg cccaattcgt 31560
cggccaagat aatttcggcg atgttcgaaa tactgtcaat acccaacgta tcgaaattcg 31620
cagcttcgcg cgatttcata aaccattcga agaattcgac gataagcgcg ggcgaatacg 31680
cttcccatgc gggaacgttc gaaccgcgca tggacagcat accgggttcg gtcacaagca 31740
aaaccggacg cggcgcagta ttgataagcg gcgttttgcc ggaaccgggc gcaccgaata 31800
cgacggactt tacgccgtag cggcgggcca gttgcgacgc cggttttaat tgcgacattt 31860
gcataacttg caccttcggg aatggccccg gcgcttgtgg gcaaccgggg cggggttgtt 31920
acttcgactt cggcgcgaca agttcaagcg cgggcgttgc ttccttcgac gttacaactt 31980
cgtcgataag cttacggaac ttgtcgggaa ggttcttgta ttcggtaacg gaaagttcgg 32040
gcttccattt taccaaacgt tcggcgataa attcgccttc ggggccggtc ttttcgattt 32100
tctgcaaagc cttttcgacg gcttcttggc cgcctgcgaa gctgcggtta atcttaaaaa 32160
cggctttcag cttgtaacct gcgccaagtt cgacgttttc ggttccttcg cgcaaagctt 32220
cggggtcgaa agcgaaagcg tttttcagaa cttcggcgcg caatgcggat tcggcttctt 32280
tcgctgcggc aagcgccttc gttgcttcct gccattgcag aatcaaagcg tcgcggttag 32340
cgttttcgtt gaaagacatt gttgttactc cttcggttgg gccgccgcac aattgcgccg 32400
gtatgtacga actatacgac ggcccttcgt tactgtcaag cccctttttc aagaattttt 32460
cgttcttcga acaagtcgcg attgttggcg tcgaattcgg taaaggcgtt cgggaagcga 32520
cgacgaagct tcgcgatgtt gaccgactgc gcttcgccga agttcgaacc aatggcgcgc 32580
agcaaaagcg catcgtacca aaagccgtcg ccgacttctt caagcgcgtt aacagcgtcg 32640
aaagcttcgc cttcggctgc ggcgcttagg gcttccaaca gttcgcccgc ttcggtcgcc 32700
ttgccgataa ttgcatgaat gacgttcacg gctgcggcgt cgtcttcggg gcggtcggaa 32760
atccattcgg gaagcttcgc cagcgttgcc gcgttggctt ccggggccgg aacgccagcg 32820
tcgcgaccgt agaacatggt tttctcgatt gcgtcaagct tgttaagggc ttcgacggct 32880
tcggcgatgg tttcgcggaa gtaggccagc gacacgcggt cgccgtaata cttatccgac 32940
gcggttacgt gcgcttcttc gatatagtcg aaggtctttg cgggttcgtt catacgttca 33000
ccttttagaa gggccgggca ccatgcgcca gcgttccagc actgtaaccg accgcgcctt 33060
tcctgtcaac aacaattgac agcaagcaag aagccgggta agctgcccac aattgctaga 33120
aggtggcgtt atggacattg aagaacttac gaagcgggta aagaaagcaa gcgtcgggcg 33180
gtcgctgcgg gcgctggccg cgcaatgcaa cgtttcgcac gaacttataa gaaagctttt 33240
gcttgccaat tctgcgccga acataacggc gacgacttat aacaagattg ataaaggatt 33300
ggcgaagaat ggactttaac aatattccgc acgaaatgcg gatttacccg caatgggtcg 33360
tttggcgata cgaagacacg gattcaaaga aaccgacgaa ggttccgtat tcagcaaaaa 33420
ccgggcacct tgccagcgta accgacccga acacttgggc cgggttcgac gaatgcgtta 33480
acgctatgtc gtccggttgg tatgccggaa tcggtttcgt attgactgaa aacgacccgt 33540
attcgtttat cgaccttgac gacacaaaag gcgaccaaac ggcgcttgac cgccaaataa 33600
agatttttaa cgaattcaac agctacgccg aacggtcgcc ttccggttcg gggttgcata 33660
tcatcgttaa gggcgcgatt ccgtcgggcc gccgtcgttc gtttatcgaa gtttattctt 33720
cgcttcgtta tatgacaatg accggcgacg tttaccgcaa cgcaccgatt aacgattgta 33780
acgaactgtt aaacgttctt tggggccaaa tggggcaagg ttcggtcgcc gtcgcgcatt 33840
atgccggact tgccgaagcc aaggaaaccg acgaacaggt ttataaccgc gcagtcgccg 33900
cagccaatgg cgacaagttc gccgaactgt acgcgggcaa atgggaaggc atgtacgctt 33960
cgcagtccga agccgacttt gccttggtcg atattatcgc gttttatacg cagaaccggg 34020
cgcagatttc gcgaatgttt cgcgcgtcgg gcttggggca gcgtgacaaa gcaaaacgcg 34080
acgattacgt ttcgtacatg ctgaacaagt gtttcgaccg catgttgccg cccgttgacg 34140
ttgacggatt gcgcaacaag ctagacgaag ccatagcgaa gaaagaagcc gccgacaggg 34200
ccgccgcgtt gtcgcagaac agcgaagcga cgccgcatcc gaaagccccg gccccgaacc 34260
ttagcgaagt ttcgaaggta tacagcgtgc cgcccggatt agtcggagaa attgcgcaat 34320
acatttacgc acaagcgccg cgcccggtgc ccgaaatcgc gttggccggt gcccttggct 34380
tggtcgccgg tattgtgggc agggcgtaca acatttccgg cactggcctt aatcaatacg 34440
ttctgttgtt ggcaccgacc ggaacaggta aagaagccat cgcaagcggc attgataagc 34500
taatggcgca ggttatccga accgttcccg ctgcgtcgga ctttatcggc cccggtgaaa 34560
ttgcgtcggc ccaagcgatt attaagtata tgtcgcgcgg cccaacgtcg ttcgtttcgt 34620
tggtcggcga attcggcatt taccttcaac aaatggcaag cgtaaacgca ccgccgcacc 34680
ttaccgggct tcgtcgcttc ctgttggatg cttacaacaa gtcgggcgaa ggcaaggtac 34740
ttcggccttc gatttattca gacaaggaca agaacacgac cgccgttctt tcgccgtcct 34800
ttaccttgct tggcgaatcg acgcccgaaa agttttacga aggtttgcac gaaggtttga 34860
tttccgaagg tctgttgccg cgttttacga tgattgaata tcacggcgaa cgcccggcgt 34920
tgaatccggg gcacctgtcg gcccaacctt cgttcgaact tatcgacagg ctttcgacct 34980
tgtgcgccca tgcgctaatg ctgaacagcc agcataaggc gattcacgtt caaaccgacg 35040
caaccgcccg cgaactgttc cagcaattcg acgcgcattg cgacgcgaac attaacacaa 35100
gcgaccgcga agttcggcgg cacctttgga accgggcgca cgtaaaggcg ttgaagctgg 35160
ccggaattat cgccgtcggc tgcaaccctt acgacccgac cattaccgcc gacgttgcgt 35220
cttgggcgat taaccttgtc gtcgccgacg ttcgaaactt gcttgcccgg ttcgatgctg 35280
gcgaaattgg catagacaac gacgaaacga aacagcttgc gaaggttatt gcaaccgtta 35340
aagatttcgt cgtttcacct tggccggacg ttgcgaagta cgcaggcgaa ggaatgtcca 35400
accttcattc gaaccgaatt gttccgtaca gctacgttca acgcaggctt gccgctgttg 35460
ccgtctttcg taaagaccgc atcggcgcaa gcggggcaat caagcgcgcc ttgaagaccc 35520
tttgcgaacg cggcgatttg caggaagtaa gccgggccac actggcgaag gattacggaa 35580
caagcgccgt tgcatacatg gtcgcgcatc cgggcgtttt cggcctgtaa cgcgcgcccg 35640
ccagtgtggg cagaacgggc cgccttcggg cggctttttc gtgcctgtaa aaaaaagttc 35700
gtcgtacctg ttgacgtagc ttcgaactgt acgtagaata caaaccatcg aagcaacgaa 35760
acaaaccaag gaaccgaagc catgaacgta accaacctta aaaccgccgt cgaaatcaaa 35820
attgaaattg aattggccgc ttgggccaac ggcgaaaaga aagttaacgt ttccaaccaa 35880
ttcgacgaaa gcgttttgga atcggccaaa gaagcaatta gcgtcgaagc aatcgccgca 35940
atcgttgcca agtatccggg cgcgaacttc gaaggctttt cggttatgac ttgggcgcac 36000
gaactttcgg cgttcaaagc cccggaaatc gaccgggctt ggttcgacta agcaagcgcc 36060
cttcggggcg ttttttttgt tcgtacctat tgacgaagct tcgaagttgc cgtatattta 36120
aaccatcgaa gcaaacaacg aaggactaac gaacatgaac gcacttaaca acattcaacg 36180
ggtcgtaagc tggaacagca agaagaacgc cgacggcacc tttaccgcta ccgcgtacag 36240
cttcgaccat aacgcgccga ccgtcgtaca tgccagctac acgcgcgcca gccgcgccca 36300
agccgtcgca gacgccaaga aggccgttcg ttacctgaaa gcccaacagc gcaccgcgta 36360
aggagtcgcc gccatgtttg cccattactt cgccagcaag aaaacgaagt tcgttgtaat 36420
cagcgaaacg aaccgcccgg aaggccagcg aatcgccgta agcggcaaag ccgaagcccg 36480
cagggtcgcc aaggaacgca acgcgcagcc ttggaacttc taaccccggc cacggccctt 36540
ggcacgcgct aggggccgcg cagcctgccc acattaagcg aaggacgacc gaccatgcgt 36600
acaacccttg cagccgtaaa caaagccctt gccgccgctg gaatcgacgc ggaattggtc 36660
aagggtcgcg ggtactttta cttcgttggt tccgccgtag attgcgccaa agaacaaggc 36720
gtttacggcg tgtatcggct ttccgaattg accgtttccc aatgggtcga agaagcccgc 36780
caacgtatcg aaaaggagta agaacaaatg accgcatatt ttatcgcatt tggcgtcgcg 36840
cttgggttgg cccttgcgcg cttggccgct tcccaattcg aagccgcttg ttacctgttc 36900
tgcaacggcg aatttcgaaa ggcattccgg gcaacgatgc acgcccttac ctgcgttgcg 36960
ttgcttgcgt tggttgtttc gcccgttgct tcgttcttcg atagcttcga agttataagc 37020
attgaacaac cttcgcccaa tccttcgaag ggtcttcgcg tataatagcc gacgggtcgt 37080
ataatgggca cgtataagcc ttaaaccctt gatacataag gctttcagcg gattcataac 37140
aagtataacg tataacgccc ttgaccctga aaccccggcc caatgttata aattaagctt 37200
tctttatata gattgatttt tagttattat acttattaaa tttattatat ttccttattt 37260
atcaaggact tagcagctat acagcccctt caattcgtat aacgggcttt ttgtgttata 37320
acgttgttga catacaaact ttataaggct atgattccgg cacgcactaa cgaaaggggc 37380
ttgcaatgtc ttacgaaata atcgaatgtt cgccgaaggc gcgaagcaaa attaagtcgt 37440
tcgaagatgc gctaggcgtt gacgccgaat tgacgcacca acgttcgaag tatccttggc 37500
cgctgttgaa aatcggccag tcgttcgccg tgccgttggc cgaaggtaac gaagcatcgt 37560
tgcgaaacgg cgcgtcgcag ttcagcaaga agaccggcaa gaagtttgcg gttattcggc 37620
attctgaata cggctgtttc gaatgcgcgc gcatcgctta gcgccgggcc aatgtgggca 37680
acaaccgaag gggcaaccaa tgtacgaaat cgaaaccggc cataaagtgc cgaagcaacg 37740
tcgcaaggct ggaagcgtgc cgaaggccgt tcaacaggcg ttcaacgact acgccgaagc 37800
gtataaggcc gtttacggcg ttcgcccgct gtcgttcacg tatgaccgcg aaagcggctt 37860
tattcgtgtc gaaagtagcg gcggcgtaag cgtcgcaagg ctgcgcgaaa tgacgaaaca 37920
gcttcgttac cgaaaaggat aaaaaaattc gttcgaaggg ttgacgtact gtcgaagccg 37980
tcgtactata tacccatacc aacgaacaca aggaaccgcc gaaatgcaaa acgtccaaat 38040
caaacgcccc gcagcgatta ccgaaggcca agcaatgaac gcagttttcg acgccttccg 38100
ccaagtgtgg cgcaacgtcg aagcccgcgc cgaaatgctt aaaggcgttt cgcagtcggt 38160
caaggtcggc ggcttcgaag tgtcggtttt cgcatggcgc gacggtcgca ttcaagccaa 38220
cgtttacgca ggcggcttta accccgtcgc aaccgccgaa gttgacgaat tcgacgccta 38280
atcaaccaag cgcgccgggc gcaacccggc atttttgaag gagttataac aatgtgcatc 38340
gtatgcgata ttaaacaggc cgtcgaagct tccggcccga acaaagaaga ccgcgccaaa 38400
atccttggct tggtcgaaaa gctggccgaa gccttgggcg atgttctgga agtcgcgcag 38460
gaagtacacg aagccaagcc cgaagcgttc cagccggaac acgtcgaacg cttgcagaac 38520
gccgccgact tgctgaacga ccggggcgaa ctgcccgacg ccctgttggc cctgctgttg 38580
gccgcccttg ggcgcggcgt caaggtcgaa acaatccgcg tcgaactgcg cgaaggcgaa 38640
acgccagaac aggccgtagc gcgtgtaatg gccgaacgcg aaggcggggc cgcgacgaaa 38700
cactaaccaa agcgccccgg ctgttgtata gtcggggctt ctttctatca tggggaacga 38760
aagaacatgg cggacgaagt agaccaaaca gccgaccgaa tagaaaacga agtaagcttt 38820
acggtcggcg aactttgccg aaaggccgca gcgatacccg caggccgtcc gggcgaatgt 38880
gatttttgcg gcgaacactt cgcgcgcgtt gtcgaagtga ccgacccgcg aacgggcgaa 38940
gtcgtcgaaa gctgcggtcg ctgtcgtgac cgaaggggca tcgcatgacg cccgcaaccc 39000
ttgccaagtc tggcaccgaa cacgcgcacc aaacggcgct ttttgcctat gctgccgtcg 39060
catacttaca cggcttcgac gttgccgacg aatggtcaaa gaccggcaag ctaccgaagc 39120
gcgacccgga cgcaccgccg aaggtgcccg cccttgaatg gttccacgcc ataccgaacg 39180
gcggcagtcg cggcgacgac gacaagtcgc gaaagatacg cggcgcgcag ttgaaggccg 39240
aaggcgtgcg gcagggcgta gccgatacgt tcttgccttg gcctgtttac gaaccgtgcc 39300
caagcgaccc gcaaataatg atgatgaaat gggccggtct ttatatcgaa atgaagaagc 39360
cgaccgaacg tccgaagtcg gaaaccgcaa agggcggcgt atcggacgaa caacgcagct 39420
tcggcgaata cgcaaagcgc gtcggctatg gttgggccgt ctgctatgat tgggaacaag 39480
cggcgtcgta ccttcgttcc tatatcgaat ggggttcgta atggggcttt cgtgccttgc 39540
ctgcgaactt acccgcgccc ggttgaaagc ggcggcgctg gcccttgtgg gctggcccct 39600
tgaccgcatc gccgggcatt tgtcgtcatg ctatggcgaa cggtattacg tcgaaggcgg 39660
caagctgtat cgcgcgtcga agttgccgcc gtatcaaccg cacttgataa ggggcgaaga 39720
atgaaaaaga ccgacgaaga attgatagac gaagcccgca gcattgcgcg ttcgaaacgc 39780
tgtttcgtcg taacgaagcc gggttattgg ctgttatacc gcgaatgcac gccgcgtaac 39840
gtatgcgtcg gcaaacggtc gtcggctgcg gcgttgcttc aacttgcgaa aaaggcttga 39900
cgtagcttcg aagctgtcgt acacttcttc gtaagttgaa cgaacaaagg agtaacgacc 39960
atgcccgcat ataacgtaac aaccgaattc gaaatgaccg acggtcgcgt tctgtcgtgc 40020
gaatacgtct tgtcggttac gcccggtcgc tattccggcc cttgggaaga ttgttacccg 40080
gacgaatccg acgcaggcga accgacgtat tttatcgacg gcgaagaagt cgattacaaa 40140
gaacttccaa aaggattgga cgcaatcgcc gacaagcttt acgaagctgg cgacggcgaa 40200
tacaattaca aggaagaacg cgacgaaccg gattacgaac cggattacga ttacgattat 40260
taacgaagga gtaacgaaaa tgtcttggct tattggttgg gccgtacttt ccgtcggcgt 40320
tgttgtcggc ttcttgcttg cggccttgct gcgggccaac gggcgcgacg tgccggttcc 40380
ttcggctacc gttaccgccc tgtcgccgca ggaacgcgcc cgcatgggct acgcgctggc 40440
aagggctggc gacttgctgg acagcggcga ccttgaatac gtgtcgggcg gctttatggt 40500
gcaagacgta ggacacggcg tcgtaattat caaaggcgat ttgtcgttgc gggttgccga 40560
agatgaataa ggacgaagcg cgcgaaatgt tcgaagccgt caaggccaac cgggcgaaga 40620
ttgacggttg cgcccgccat cgcttcgaaa tcggcgaccc gccgtacacg ttcgggcaaa 40680
agttcacttg ttcgaactgc ggcggaacaa tggacgccgt acaggcgttc cgatactgtc 40740
aaggcttcaa ggccgcaggc ggcgacccga acgaagttat accgggttac gaataacgct 40800
tgacaagcgg cgaaggttcg gcgaaactta gacgtaacga agaaaccccg ttaaggagtt 40860
gccgaaatga ccgttctaat tttgcttctt tgcaccgttg ccgctttgtt cattcatcgc 40920
atgtttgttc gtccgattct ggattggttc gaacaacgcg aatacgccga acgtatgcgc 40980
gaagctgaac agcgcgcggc gttggaagaa gcaaggcggg ccaagtgtcg ataatctacg 41040
caccgcgcga cgccctgtcg aaatgggacg cgcgatttat tgaactggcc gaacacgtcg 41100
ccggatggtc gaaagggccg cgtaagcgca tcggcgcggc tatcgtgcgc ccgaatcggt 41160
cgattgcgtc gcttgggtac aacggcccgc cgcgcggctt cgacgacgcc gtttttctga 41220
acatgacgcg cgacgaacaa cacgcggtcg taattcatgc cgaagacaac gcaattcgac 41280
aggctgcgcc gggcgaagac ttgtccgcgt gtacgctgta cgtttcgccg ctgtttccgt 41340
gcgccatttg tgccgaccgc attgttcggg ccggtatccg tcgcgttgtc gcttattgtg 41400
ggcatatatc gccagactgg cgcgcgtctg ccgacgaagc cgaacgcatc tttaaccaag 41460
cgggcgtcga atgcctgttt acaatggatt aacgaacatg aaaccgctag accattcgca 41520
aatcgaaggc ttgttgttct atcaagaccc gacaacgcaa aactatatcg gcgcaccgaa 41580
ggccgacccg ttcaaccaag ccggaagctt cgtcgcattc gggccgcgtg ttaccgttga 41640
cccggcgttg tataacttgc ttgccgcagc gccgacaatg taccaagccc tttcgatgca 41700
atacgcttac cttcaagcgc aaatcgaagt tatcgaacgc ttgccaaagg cgggcgaact 41760
tgataagctg ttgtcgtcgc taatcgaagc gcagaacgct tgcctaatgg cccaacgtat 41820
cgcgcaagtc ggttacgaaa atgtcgcaaa ttcacttgac aaaggttcga agcggtcgta 41880
agcttgaacc atcgaaacga acaaaggtgc ccacaatgaa ccgtaagctt cttgttatcg 41940
ctggaatcgt cgccttcgtc gcgttgcttg gcatggtcgg gcaaatggac ttcgaagacg 42000
aagcccgcgc gcacctgttg tattgtgaca acgtagcgaa cggcgtttgg ccggattacg 42060
acaaaattta cgattctgaa tgcacgcccg aacgcatcgc agaaatcgaa gaaattttgc 42120
gttgaccgct tgacacgaag aacggtcggc gtaaaatcaa cccgtcgcaa caacgcgaca 42180
taaccttaac gttaaggagt tacacaaaat ggctaccaag aaatcgaccg cgaagaagaa 42240
caccgccgcc ccggttatcg gccttgccga aattgttgca gccgggccga atggtctgta 42300
tacttcgcct gccgtacatg gcccgcttgt cgaagccggt atggtggaaa tcaacccggc 42360
aatgaccaac gaagcgggcg aaatcgcaac ccgcgcaacc caaaaaggaa tcgacgaaat 42420
gaaccagaac accgaaaccg gcaccgccgc agcttccgcc ccggtcgcta cttccttcgc 42480
catcgaagac aacgtaccca tgccgaccgg cagcggtcgc ggtcgcggcg gcaacgtcta 42540
cccgttcgac gcgctggaag tcggccaaag cttcttcgtt ccgaacagcg aagaaaagcc 42600
gaacgccgcg aagtcgcttg cttcgaccgt ttccagcgca accgcccgtt tcgccgtgcc 42660
ttccgaagac ggcgcgacca agaccaacaa gaagggcgaa accgtgcccg tcatggtcga 42720
aacccgcaag ttcgttgttc gttcggtcga aggcggcgcg cgcgtctggc gcaccaagta 42780
agcgacggcg cgggctacgg cccgccctac gcctacgctt caacggaaac cccggccatt 42840
gcgccggggt tttcttttgc ttttcttgtc aatttgtgct aatccgtgct aatcttcggc 42900
caaatacatt tcggggccgg ggcttgtgat ga 42932
<210> 14
<211> 42932
<212> DNA
<213> Artificial Sequence
<220>
<223> s4 phage变体
<400> 14
tcgaagggtt gacgtactgt cgaagccgtc gtactatata cccataccaa cgaacacaag 60
gaaccgccga aatgcaaaac gtccaaatca aacgccccgc agcgattacc gaaggccaag 120
caatgaacgc agttttcgac gccttccgcc aagtgtggcg caacgtcgaa gcccgcgccg 180
aaatgcttaa aggcgtttcg cagtcggtca aggtcggcgg cttcgaagtg tcggttttcg 240
catggcgcga cggtcgcatt caagccaacg tttacgcagg cggctttaac cccgtcgcaa 300
ccgccgaagt tgacgaattc gacgcctaat caaccaagcg cgccgggcgc aacccggcat 360
ttttgaagga gttataacaa tgtgcatcgt atgcgatatt aaacaggccg tcgaagcttc 420
cggcccgaac aaagaagacc gcgccaaaat ccttggcttg gtcgaaaagc tggccgaagc 480
cttgggcgat gttctggaag tcgcgcagga agtacacgaa gccaagcccg aagcgttcca 540
gccggaacac gtcgaacgct tgcagaacgc cgccgacttg ctgaacgacc ggggcgaact 600
gcccgacgcc ctgttggccc tgctgttggc cgcccttggg cgcggcgtca aggtcgaaac 660
aatccgcgtc gaactgcgcg aaggcgaaac gccagaacag gccgtagcgc gtgtaatggc 720
cgaacgcgaa ggcggggccg cgacgaaaca ctaaccaaag cgccccggct gttgtatagt 780
cggggcttct ttctatcatg gggaacgaaa gaacatggcg gacgaagtag accaaacagc 840
cgaccgaata gaaaacgaag taagctttac ggtcggcgaa ctttgccgaa aggccgcagc 900
gatacccgca ggccgtccgg gcgaatgtga tttttgcggc gaacacttcg cgcgcgttgt 960
cgaagtgacc gacccgcgaa cgggcgaagt cgtcgaaagc tgcggtcgct gtcgtgaccg 1020
aaggggcatc gcatgacgcc cgcaaccctt gccaagtctg gcaccgaaca cgcgcaccaa 1080
acggcgcttt ttgcctatgc tgccgtcgca tacttacacg gcttcgacgt tgccgacgaa 1140
tggtcaaaga ccggcaagct accgaagcgc gacccggacg caccgccgaa ggtgcccgcc 1200
cttgaatggt tccacgccat accgaacggc ggcagtcgcg gcgacgacga caagtcgcga 1260
aagatacgcg gcgcgcagtt gaaggccgaa ggcgtgcggc agggcgtagc cgatacgttc 1320
ttgccttggc ctgtttacga accgtgccca agcgacccgc aaataatgat gatgaaatgg 1380
gccggtcttt atatcgaaat gaagaagccg accgaacgtc cgaagtcgga aaccgcaaag 1440
ggcggcgtat cggacgaaca acgcagcttc ggcgaatacg caaagcgcgt cggctatggt 1500
tgggccgtct gctatgattg ggaacaagcg gcgtcgtacc ttcgttccta tatcgaatgg 1560
ggttcgtaat ggggctttcg tgccttgcct gcgaacttac ccgcgcccgg ttgaaagcgg 1620
cggcgctggc ccttgtgggc tggccccttg accgcatcgc cgggcatttg tcgtcatgct 1680
atggcgaacg gtattacgtc gaaggcggca agctgtatcg cgcgtcgaag ttgccgccgt 1740
atcaaccgca cttgataagg ggcgaagaat gaaaaagacc gacgaagaat tgatagacga 1800
agcccgcagc attgcgcgtt cgaaacgctg tttcgtcgta acgaagccgg gttattggct 1860
gttataccgc gaatgcacgc cgcgtaacgt atgcgtcggc aaacggtcgt cggctgcggc 1920
gttgcttcaa cttgcgaaaa aggcttgacg tagcttcgaa gctgtcgtac acttcttcgt 1980
aagttgaacg aacaaaggag taacgaccat gcccgcatat aacgtaacaa ccgaattcga 2040
aatgaccgac ggtcgcgttc tgtcgtgcga atacgtcttg tcggttacgc ccggtcgcta 2100
ttccggccct tgggaagatt gttacccgga cgaatccgac gcaggcgaac cgacgtattt 2160
tatcgacggc gaagaagtcg attacaaaga acttccaaaa ggattggacg caatcgccga 2220
caagctttac gaagctggcg acggcgaata caattacaag gaagaacgcg acgaaccgga 2280
ttacgaaccg gattacgatt acgattatta acgaaggagt aacgaaaatg tcttggctta 2340
ttggttgggc cgtactttcc gtcggcgttg ttgtcggctt cttgcttgcg gccttgctgc 2400
gggccaacgg gcgcgacgtg ccggttcctt cggctaccgt taccgccctg tcgccgcagg 2460
aacgcgcccg catgggctac gcgctggcaa gggctggcga cttgctggac agcggcgacc 2520
ttgaatacgt gtcgggcggc tttatggtgc aagacgtagg acacggcgtc gtaattatca 2580
aaggcgattt gtcgttgcgg gttgccgaag atgaataagg acgaagcgcg cgaaatgttc 2640
gaagccgtca aggccaaccg ggcgaagatt gacggttgcg cccgccatcg cttcgaaatc 2700
ggcgacccgc cgtacacgtt cgggcaaaag ttcacttgtt cgaactgcgg cggaacaatg 2760
gacgccgtac aggcgttccg atactgtcaa ggcttcaagg ccgcaggcgg cgacccgaac 2820
gaagttatac cgggttacga ataacgcttg acaagcggcg aaggttcggc gaaacttaga 2880
cgtaacgaag aaaccccgtt aaggagttgc cgaaatgacc gttctaattt tgcttctttg 2940
caccgttgcc gctttgttca ttcatcgcat gtttgttcgt ccgattctgg attggttcga 3000
acaacgcgaa tacgccgaac gtatgcgcga agctgaacag cgcgcggcgt tggaagaagc 3060
aaggcgggcc aagtgtcgat aatctacgca ccgcgcgacg ccctgtcgaa atgggacgcg 3120
cgatttattg aactggccga acacgtcgcc ggatggtcga aagggccgcg taagcgcatc 3180
ggcgcggcta tcgtgcgccc gaatcggtcg attgcgtcgc ttgggtacaa cggcccgccg 3240
cgcggcttcg acgacgccgt ttttctgaac atgacgcgcg acgaacaaca cgcggtcgta 3300
attcatgccg aagacaacgc aattcgacag gctgcgccgg gcgaagactt gtccgcgtgt 3360
acgctgtacg tttcgccgct gtttccgtgc gccatttgtg ccgaccgcat tgttcgggcc 3420
ggtatccgtc gcgttgtcgc ttattgtggg catatatcgc cagactggcg cgcgtctgcc 3480
gacgaagccg aacgcatctt taaccaagcg ggcgtcgaat gcctgtttac aatggattaa 3540
cgaacatgaa accgctagac cattcgcaaa tcgaaggctt gttgttctat caagacccga 3600
caacgcaaaa ctatatcggc gcaccgaagg ccgacccgtt caaccaagcc ggaagcttcg 3660
tcgcattcgg gccgcgtgtt accgttgacc cggcgttgta taacttgctt gccgcagcgc 3720
cgacaatgta ccaagccctt tcgatgcaat acgcttacct tcaagcgcaa atcgaagtta 3780
tcgaacgctt gccaaaggcg ggcgaacttg ataagctgtt gtcgtcgcta atcgaagcgc 3840
agaacgcttg cctaatggcc caacgtatcg cgcaagtcgg ttacgaaaat gtcgcaaatt 3900
cacttgacaa aggttcgaag cggtcgtaag cttgaaccat cgaaacgaac aaaggtgccc 3960
acaatgaacc gtaagcttct tgttatcgct ggaatcgtcg ccttcgtcgc gttgcttggc 4020
atggtcgggc aaatggactt cgaagacgaa gcccgcgcgc acctgttgta ttgtgacaac 4080
gtagcgaacg gcgtttggcc ggattacgac aaaatttacg attctgaatg cacgcccgaa 4140
cgcatcgcag aaatcgaaga aattttgcgt tgaccgcttg acacgaagaa cggtcggcgt 4200
aaaatcaacc cgtcgcaaca acgcgacata accttaacgt taaggagtta cacaaaatgg 4260
ctaccaagaa atcgaccgcg aagaagaaca ccgccgcccc ggttatcggc cttgccgaaa 4320
ttgttgcagc cgggccgaat ggtctgtata cttcgcctgc cgtacatggc ccgcttgtcg 4380
aagccggtat ggtggaaatc aacccggcaa tgaccaacga agcgggcgaa atcgcaaccc 4440
gcgcaaccca aaaaggaatc gacgaaatga accagaacac cgaaaccggc accgccgcag 4500
cttccgcccc ggtcgctact tccttcgcca tcgaagacaa cgtacccatg ccgaccggca 4560
gcggtcgcgg tcgcggcggc aacgtctacc cgttcgacgc gctggaagtc ggccaaagct 4620
tcttcgttcc gaacagcgaa gaaaagccga acgccgcgaa gtcgcttgct tcgaccgttt 4680
ccagcgcaac cgcccgtttc gccgtgcctt ccgaagacgg cgcgaccaag accaacaaga 4740
agggcgaaac cgtgcccgtc atggtcgaaa cccgcaagtt cgttgttcgt tcggtcgaag 4800
gcggcgcgcg cgtctggcgc accaagtaag cgacggcgcg ggctacggcc cgccctacgc 4860
ctacgcttca acggaaaccc cggccattgc gccggggttt tcttttgctt ttcttgtcaa 4920
tttgtgctaa tccgtgctaa tcttcggcca aatacatttc ggggccgggg cttgtgatga 4980
atgaacgaac aacaattttt taacgtagcc gtcgcaatta tcggcgcgtt gggcggttgg 5040
tggatgcgcg ttatgtggca gtcacttaaa gaacttcaag cgcaagattc gaagttggcc 5100
gataaagtcg gcaatatcga agttcttgta gccgggcagt atgtcaagcg cgacgatatg 5160
aaccgcgaca taatggcgat tttcgccaaa cttgaccgca tcgaagacaa gatagacaaa 5220
aaggccgaca aatgaagaag cccgaactta tcgaagattg gcgcgaatgc tggcgctttt 5280
ggtcggttcg actggccgca gtcggcaccg caattactgg ccttcttgtt gcgtttcccg 5340
acgcagcgtt ggccgcttgg gcaatccttc cgcacgaatt gaagtcgtcg attccgccgc 5400
agtatatgcc gcttatcggc gttgttgttt tcgcgctttc catcgttgcc cgccttgtca 5460
agcaaaagaa cttgaacaag gggccgaagg atgaataacg aacttccttg ggtagccgaa 5520
gcccgaaagt atatcggcct tcgcgaagac acttcgaaga cttcgcataa cccgaagctt 5580
cttgccatgc ttgaccgcat gggcgaattt tccaacgaat cccgcgcttg gtggcatgac 5640
gacgaaacgc cttggtgcgg tctgttcgtc ggctattgct tgggcgtagc cgggcgctac 5700
gtcgtccgcg aatggtacag ggcgcgggca tgggaagccc cgcagcttac caagcttgac 5760
cggcccgcat acggcgcgct tgtgaccttc acgcgaagcg gcggcggcca cgtcggtttt 5820
attgtgggca aggatgcgcg cggtaatctt atggttcttg gcggtaatca gtcgaacgcc 5880
gtaagtatcg caccgttcgc agtatcccgc gtaaccggct atttctggcc gtcgttctgg 5940
cgaaacaaga ccgcagttaa aagcgttccg ttcgaagaac gttattcgct gccgctgttg 6000
aagtcgaacg gcgaactttc gacgaatgaa gcttaaaggg gcatcgcatg ttttccgtaa 6060
tttgggccaa gtttggcaaa tggcttgccg caatcggcgc gggtcttgtc atgctggcaa 6120
gtatttttta tgccggtcgc aagtccggcg caaccaaggc cgaagccaac gcagcaaacg 6180
aacgggcaaa cgaccgcgaa gcaatcgcag ttcgccaagt taacgaagcc cgcgaagctt 6240
ccgaacaaca ggtaaaggcg gtacaaaatg cgaaagacgt tgcttccgat aattctactt 6300
ttgacgacga cgaagttact aagcggctgc gcgacgaatg gtcgcgcgac tgaaccgcaa 6360
agcatcgccg acgcagtgca aacgaagccc gttgtaatcg acacggcttg caaatgggtt 6420
gcgcctattt ggatttcgaa ggccgacggc ttgaccgctg gcacggcccg gcaaattctg 6480
aaccataacg aagccgtcga acgcaattgc gggccgcagt cgccgcctaa atctgcggac 6540
aagccttaac agaacgcggg gcttgcggta tagtgaagcc aacgacgtat aaactaggcc 6600
gacactatgt ccgacgaact gaaagaaagc gaagaaaagg ccgcatacgc ggcccttctt 6660
ttgaaagaac gcgacccgtt caaggctgcg ctttcgctgt ttccgaacaa cacgaatcgc 6720
gcgctatggg ttgcgaacca ttggccgaac gacgccgaag ttaaggccga acaaaagcgc 6780
cttaccgacg aaggcggcga tatggcattt cttccgggca aaggcgacct tgcccgcgac 6840
atttggcagc gtatgcaagg tacgacgctt ccgaacggcg taaccattcc gccgacgccc 6900
gaagaatacg cgaagcttgc caagctttac gccgacgttc gcgggttcat cgaaaaaccg 6960
caaacgaacg ttaacgtaac gacgaacgta aaccgcgtcg tcgaaatgcc cgtattcgct 7020
aacgaaagcg aatgggaagc ggaagccgca cggcaacagc gcgaattgtt agaaaatgca 7080
cgcactcgcc attgatacga acggggcgaa acctgttccg tatgaagtcg tatttaagcc 7140
ccttccgggg tcgcagacta tcgccctttg ttcgatggca gcgcatacgc tgtacgaagg 7200
cgcacgcggc cccggtaaga cgcttacgca gcttatgcgc ttttatcgta acgtcggcaa 7260
aggctacggc aagttctggc gcggcgttat cttcgatttg gaatttgacc atttgggcgg 7320
ccttgttgcc gaatcgaaaa aatggttcgg cgataacgga aagctaaaag acggcggcaa 7380
gttttacgaa tcaacgtcgg cttataaatg ggtttggccg acaggcgaag aactgttgtt 7440
tcggcacgtt aagaagctgt cggattacga aggcttccac ggtcacgaat acccgtttat 7500
cggttggaac gaacttacga agcatccaag cggcgacctt tacgacaaat ttatgtcggt 7560
caaccgctgt acgttcgacc cgataaaaga cacgccgaaa gacccgaaaa ccgggcgcta 7620
tttgacgccg aacggcgaac cgctgccgcc tgtaaagtgc gaagtattca gcacaacgaa 7680
cccaagcggc cccggtcaca attgggtaaa acgtcgcttt attacgattg ccccgcgcgg 7740
caccgtagtt cgtcgcgaaa ttcagattta caacccggcg accgaaaaag aagaaacgca 7800
cgtaattagc caaatcgcta tcttcggttc gtataaggaa aacccgtacc ttccggcttc 7860
gtatatcgcc gaactggaaa gtatcaaaga accgaacttg cggaaggctt ggctttacgg 7920
cgattgggac gttaccgcag gcggcgcaat cgacgacctt tggcaatcgc atatacacgt 7980
tgtaccgcgc ttcgtcattc cgccaagctg gcgaatcgac agaacatacg acgacgggtc 8040
ttcgcatccg tttagtgtgg gctggtgggc ggaagcggac ggcaccgaag cgaccatagt 8100
tctttcggat ggcaccgaat ttacgttttg cccgcaaccg ggttcgcttg ttcagttgtt 8160
cgaatggtac ggatgcgcga aagacgaaaa aggcgaatac ataccgaata aaggtttgaa 8220
gctttcggcg tcgaatatcg cacaaggtat aatcgaccgc gaaatttcgc taatggcgaa 8280
cggttggatt tattcgcagc cttggcccgg ccctgccgac aaccgcattc gccaagtaat 8340
cgacagcgaa ctagacacga ccgaaaagct tatgtcgaag aagggcgttc gttggacgga 8400
atcggacaaa tcgccgggtt cgcgcgtaat cgggttgcag cttttccgcg accggctaga 8460
agcgtctgtt aatcgcgaag ggccgggtat atactttatg tcgaattgcg ttgcaagtat 8520
tgatttgctg cccacactgc cgcgcgacga aaagaagatt gacgacgtag acacgaccgc 8580
cgaagaccat tgttacgata tggtgcgtta tcgcgtattg aaaggcgcga acaaagcggc 8640
agcgaaattc aaacttgtta tgccaactta aaggaaccca aatcatgccg aacgtttcat 8700
ttattcgccc cgaacttggt aagctgctgc cgctgtatta cctgattcgc gacgcaatcg 8760
cgggcgaacc aacggtaaaa ggcgcgcgga cgacttatct tccgatgccg aacgccgaag 8820
accaatcgaa agaaaataag gcgcgatacg aagcgtactt gaagcgggcc gttttctata 8880
acgtagcccg tcgaaccttg ttcggcttgg tcggtcaagt gttcatgcgc gacccggttg 8940
taaaggttcc cgcgctgttg aacccgcttg tcgcaaacgc gacgggttcg ggtatcaacc 9000
ttacgcagct tgcgaaaaag gccgtatcgc tgaaccttgc gtattcccgc gccgggctgt 9060
tggtcgatta cccgacgacc gaagccgaag gcggcgcgtc gattgccgac cttgaagccg 9120
ggcgcattcg tccgaccctg tacgtatatt cgccgaccga aattatcaat tggcgaacga 9180
ccgaccgggg cgcggaagaa gttttatcgt tggtcgtact gttcgaaact tggtgcgctg 9240
ccgatgatgg ttttgaaatg aaaacgtcgg ggcaattccg cgttttgcgt ttggacgaag 9300
aaggttatta cgtccacgaa atttggcgcg aaccgcagcc gaccaaagcc gacggtacga 9360
agattccgaa aggcaattac caacaataca cggtttacaa gccgacggac gcaaacggca 9420
atcgtcttac cgaaattccg tttatgttca tcggttcgga aaacaacgat tcgaacccgg 9480
acaacccgaa cttttacgac cttgcgtcgc tgaatatggc gcattatcgc aattcggcgg 9540
attacgaaga aagttgttat atcgtcggac agccgacgcc ggttcttatc ggacttaccg 9600
aagaatgggt tacgaacgtt cttaaaggtt ccgttaactt cggttcgcgc ggcggtattc 9660
cgcttcctgc cggggccgac gcgaagttgc ttcaagcatc cgaaaacacc atgcttaaag 9720
aagcaatgga taccaaagaa cgccaaatgg tcgcacttgg cgcaaagctt gtcgaacaaa 9780
aggaagtgca acgcacggca accgaagccg aattggaagc cgcttcggaa ggttcgacgc 9840
tttccagtgc aaccaagaac gtttcggcgg cctttgaatg ggcgttgaaa tgggcggccc 9900
gttgggtcgg tcaagccgac agcggcgtta agttcgaact gaatacagat ttcgacattg 9960
cccgcatgac gccggaagaa cgccgttcgc ttgttgaaga atggcagaaa ggcgcaatta 10020
ccttcgaaga aatgcgaacc ggcttgcgta aagctggcgt cgcaaccgaa gacgacgcca 10080
aggctaaaga aaaaatcgcc aaggataccg ccgaagcaat ggcccttgct gccccggcta 10140
acgtgccggg cgatggcagc ggcggcgata atgtgggcaa caacggataa ggggcgcaat 10200
catggcacta tcggacaata agcgactgta cgatattgcg acgcgccttg ctgtttacgt 10260
cgaagacgtg aaagtttggc agtcccgaca attcgggttc gtacttcgcg aagtaaactt 10320
ggaattaacg cgccttcttg gtcgtgttcg ttataagacc cttgacggtc tttcgaaggc 10380
gcagttaaac aagcttgttt cggaattacg ggaatcgcaa tcgaagattt acagcgcata 10440
cacgcaaacg ctgcttgaac agttaaagga attcatgcgg gccgacttgg aagtaaaccg 10500
gcgcgcttgg gttacgggtt atatcgaact tgacggcgaa gaaagcgacg acattatttc 10560
ggacgaagaa gcgatacagt ttcttttaga agtaccaaac gccgattcga accctttgtt 10620
cggtttggcc gctgttacag gaagcgacga acgcatttgg tcgcaagtta cgaatacgcc 10680
aattccggca aatggtttgt acttgctgcc gtttatcaag acgtttacga attcggcaca 10740
agctggcgtc gaaagcatga ttcgcaaagc ttgggcgaac cgttggacgg ttgaagaaac 10800
cttaacggcg cttgtcggcg atggaaccgc agcgcagggc acgccgtcgc agttgcaccg 10860
ggtcaacgcg caagccgcgt cggtaatcca tacggcaacc gcgcacgttg cggcggtcgt 10920
tgcggctggc gttatgtctg cggttttcgg tcgttacgtt tggtattcgg taatcgacgg 10980
aagaacgacg gacatttgca taagtcgaaa tcgccgaatc tatcgcttcg gcgaagggcc 11040
gttgccgccc gcgcatatcc gttgccggtc gcacgttgcc ccggcaaata ccgctagtga 11100
cttggccgaa gaaacgtttt atacttgggt tgcccgtcaa cctgcccaag tgcaagacga 11160
catattaggc gacgaaggcg gcgaagcttt gcgcgatggt cgcttaaagg cgaaggatat 11220
tccgaagtat gatgcggaca ggccgctaac ttacgaagaa ttccgacgca agattaaaga 11280
aattctttcc cgctgattcg gtgaatcggc ataaccgcaa ggagtccttg aaatggcact 11340
gaaaaagaaa cttaccaaag aagaacacgc gaagctttcg gatgcactga aagccgaata 11400
tatcgaagac ggcgacggct tccgccttga cgttgacggc gacgaagata cgggcgcttt 11460
gaagcgcgcc aaagaccgcg aagcgcagtt gcgcaaagac gccgaaaagg aagcgaagga 11520
acttcgcgaa cggctggaat ccatcgaagg cgacgacgcg cgcaagaaag gcgacattgc 11580
gacgcttgaa aagtcttggc aatcgaagct tgaaaaacag cgcgaagaat acgaagccaa 11640
ggtttccaag cttaccgcgc atacgacgaa aacgcttgtc gataacgtcg cgtcgcagct 11700
tgcccacaag atttcgaacg ccccggcaat catcatgccg catatcaagt cgcggcttat 11760
cgcagacttc gaaggcgaca cgccggttac gcgcgttctg gacaaggacg gcaagccgtc 11820
ggcccttact atcgacgaac tggcaaacga attcgttgca aacaaggatt tttctgctat 11880
tatcacagct agtaaggcgt ccggcggtgc cggtaagcct tcgcagaacg gcggcggtgc 11940
cccgaaattt aacggtcaat ccgacaaacc cgccgacctt tcgaagatga atcccgcaga 12000
acttgcggcg catcttaaag aagcgaaggc aaacgaaact aaggacgctt aatcatggca 12060
ctttccgacc ttgctgttta ctccgaatac gcttattcgg ctttctccga aaccctgcgc 12120
caacaggttg atttgttcaa cgctgcgacg ggcggggcta tcatgctgca atccgcagcg 12180
caccaaggcg acttttccga cgttgccttt ttcgccaagg taacgggcgg ccttgttcgc 12240
cgtcgtaacg cctacggttc cggcaccgtc gccgaaaagg tcttgaaaca ccttgtcgat 12300
acgtcggtta aggtcgcagc cggtacgccg cctgttcgcc ttgaccccgg ccaattccgt 12360
tggattcagc agaacccgga agtcgcaggc gcggcaatgg ggcagcaact cgccgtcgat 12420
acgatggcgg atatgctgaa cgttggtctt ggttcggtct attcggcact ttcgcaggtt 12480
gcggcggttg tttacgacgc gaccggcaat actgcgcccg acgacggccc gacttggaac 12540
aaccttaaca acggccaagc caagttcggc gaccaatccg cgcagattgc ggcatggatt 12600
atgcacagta cgcccatgca caagctgtac ggtaacaacc tgaacaactc cgaacgcctg 12660
tttacttacg gcaccgtgaa cgttgttcgc gacccgttcg gcaagcttct tgttatgacc 12720
gattcgccaa acctgttcga agccggtacg ccgaacgttt atcgcatcct tggccttgtg 12780
ccgggcgcgg ttatgattgg tcaaaacaac gacttcgacg cgaacgaaga aaccaagaac 12840
ggcgacgaaa acattattcg gacttaccaa gccgaatggt cgtacaacgt cggcgttaag 12900
ggcttcgctt gggacaaggc gaacggcggc aagtcgccga ccgacgcggc gttgttcact 12960
tcgaccaatt gggacaagta cgcaacttcc gaaaaggatt tggcgggcgt tgtcgtcgaa 13020
gttcactaac ggacggcaga cggacggggc ttcggccccg ttcttctagt ccttcacaaa 13080
ttcaaaggag tttcgaagat gaaaccggca aaaatcctgt atttcgtcga tggcaacgca 13140
ccgaccccgg aagacttcgc agccgccgcc gaactgaacg cgcaagttat gttcaggaac 13200
gcccgcgctg ttccgtccga accgcattcg ctggaaatct gcgacggcgt agcgggcaag 13260
gttccgaagc tgtacgccga cgcttacccg gaagcgggcg aagcaatcaa gaagaaggcc 13320
gccgaactga aagcccttgc ttcgaaggtc ggcgacgccc cggcaccgaa ggccagcggc 13380
aaggccgccg acaagcccgc agccgctacg cagggccaga aagccccggc agcggccccg 13440
cagggcggca gcgccccggc ttggaaccct aacccggcgc agtaaccaac ggacggcctg 13500
tcgcttattg tgggcaggcc gtttcaacaa cgaactaagg agtcttcgca atggcaacga 13560
aaaaggttgt ttattttacg gcaggcatta ccgcaaccgc tggcgaactt gccgcaattg 13620
cgaagcttaa cgcagccgcc gaaccgcaat atgaagtttt ggtagccaat ggcgcggcaa 13680
atgccgaata cggcgaaacc gaccgaatca ttccgtcgga ttatgtcgcc ggtactgttc 13740
cttcggaata ttcggcggtt gacgtaatcg accccgacgc aatcccgaat caggcgctta 13800
cgccaacgca agcaatcgtt aacgacgccg aagccttgac cgttccggtt actggcactt 13860
acacgacgac cgcaaccgtt agcgttgcga acggcgttgt aaccggcatc gttctttcgt 13920
aaggacgttg acgcatggca attacaatcg ttgttgaaga cggaagcggc gtaacaaacg 13980
cgaacagtta cgtaagcgtc gcagacgcgc gcatttacgc ttcgaatcgc ggcgtcgaac 14040
ttccgttgga cgacgacgaa ttggcggcca tgctgattcg ttcgacggat tacctagaag 14100
cgcaggcttg ccggttccaa ggcaagccaa cttcgacgac gcaggctttg caatggccgc 14160
gaactggcgt ctttcttaac gaagacgaag taccgtcgaa cgttattccg aagtcgctta 14220
ttgccgcgca agttcagctt gcaatggcga ttaacgcagg cttcgacctt caaccgaacg 14280
tttcgccgca agactacgtt acgcgcgaaa aggtcgggcc gattgaaacg gaatacgccg 14340
acccgctgtc ggttggcatc atgcccacat ttaccgcagc gaacgcactt cttgcgccgc 14400
tgtttggcga atgcgcttcg aacaagtttg cgcttcgtac aataagggtt tgacgaatgg 14460
cacgtttcga cgcagcaatt aaaacggcgc aacgccttat cgcaaagaac ggcgaaaagg 14520
tgaaatggcg cgttatcgaa gacgcgacgc cgacagaccc gaacaagcct tgggaacccg 14580
gcccggcatt gccggacgac aaagacgtta ccatttgctt tttgccggtt gaccggcaga 14640
cgcaagaaac tttcaacttc attaaaggca ccgaagttcc gaaaggttcg gtaatggggc 14700
ttatgggcaa tgttccgttt gaaccgaatt tgaaagacgt tgtaattcgt aacggggtcg 14760
aacttcgctt ggcttatatc gacgtacttt cgccgaacgg gcaaaaggta ctttatacga 14820
tggtatttca agcatgattg aattcgacca agttaacgac gaagtaaacg cgctttttct 14880
tgcaacttgg aacgcaggaa gcgcggcaat tgcgggttat gttcccgaaa ttcgttggca 14940
gggcgtgcaa tatcgcgact tgccggacgg ttcgaagttt tgggttcggc tgtcgaagca 15000
aaccgttttt gaagaacaag cgaccctttc aacctgcgaa ggagtaccgg ggcaaagaaa 15060
atatacggcg tcgggtcttg tcttcgtaca aatcttttgc ccgaaatcga atacgcaagc 15120
gttcgaactt gggcagaaat tggcgaaact ttcccgtaat gcttttcgcg ggaaatcgac 15180
gccgggtaag gtttggtttc gcaatacgcg aattaacgaa cttccgcccg aagaacttta 15240
cgaacggttt aacgtcgtta ccgaatttga atacgacgaa ataggttaag gagttcttaa 15300
catggtatgc gaaattgcca aaatcgacag caacattacc ggacttgcct tcgccgaaga 15360
agaatgcttg aagcaacttc cgacgacgcc cgtttggtac ggactggaac ccaacagcta 15420
ttcggacttc ggcggcgaac tttcgaccgt tgcccgcgcg cctatcgacc cgtcgcgcca 15480
gaacaagaaa ggcacgatta ccgaccttga cgcatcgggc ggcttcaacg ccgactttac 15540
gaagaccaac cttacgcgaa ttctgcaagg cttcttcttc gcagacgcgc gcgaacttcc 15600
ttcgacgcag ccgcttaacg gcgcatcggt cgcacttacc ggcgttaccg cagttgacag 15660
cacttacgcc gcagcttccg ggcttggcgt gttcggtgcc gatatgctgg tatatgcgac 15720
cggctttacc aacgcggcga acaacggcct taaaaccgtc gtttcggcta ccgctgcggg 15780
cgtcgttgtt gctgaaacct tgattgacga aaccccgccc gcaggcgcga agctggaatg 15840
cgtcggtcgc cagcttgccg ccgcagacgc gaacattgcc gtaactggca acgtcgtttc 15900
gcttatcgtt accgctggcg actttacgac catgcccgaa ctgttccccg gtcgttgggt 15960
cttcgtcggc ggcgatgcga cggcaaaccg cttcgctaat aatgtgggct atgcccgcat 16020
taagtcggtt gcagcgaagg cgcttgtttt cgacgacgtg acttggcagg cggccaacga 16080
aaccggaacc ggcaagtcga ttcgtctttt cgtcggaact gttatcaaga acgaaaagac 16140
cccggcactt atcaagcgtc gttcgtatca aatcgaacgc accttgggcg aaggtttgaa 16200
cggtacgcag tgcgaatatc tggaaggcgc agtaccgaac gaatttacgt tgaacgttcc 16260
gcaggccgac aagctgaacg ccgacctttc gtttgttgcg tgcgacaaca cttaccgcag 16320
cggcgacccc ggcgacgaac agaaaaccgg aacccgcgtg cctgcgcccg gcgaagacgc 16380
ttacaacact tcttcggacg tttaccgaat caagatggcc gttcacgacg ccgcgtcgtc 16440
gaaccccgcc gccctgttcg gctacgtttc cgaagcgaac gtttcgatta acaacaacgt 16500
tacgccgaac aaggcggtcg gcgtgttggg cgcgttcgat acttcggcgg gtaacttcga 16560
agtcggcggt tcgattaccg cttactttac gaccgtcgca gcggttaagg ctgttcgcgc 16620
gaacgccgac gttggtttgt cggtaatcag tgcggccaag aacgccggtt tcgtgttcga 16680
tattccgttg cttggtttgg gcggcggtcg tctgaacgtc gaaaaagacg cgccgattac 16740
cgttccgctg gaacccgcag gcgcagaaaa cgcgaacggc tatacgatgc tgtacgaagt 16800
gttttcttat ctgccgaatc tggcaatgcc ggactaattg cggtaaactc aaagggccgg 16860
gtaataccgg ccctttcttc attcaatcgg agtaaatcaa atgtctggac tgtttaagca 16920
attcaaaacg aattcgacca aggaagtcga aggcgtcgaa atcgaatttc ccgaagcgca 16980
gaacgacgac ggcagcgttc cgactttcgt tatttcccgc atgggcaaat cgaacaaggc 17040
gtattcaaag gcgcttgacg cggcgacccg tccttatcgc cgtcaagtcg aacttggcac 17100
gctgaaaaac gaagtcgccg aatcgctgtt tatgggcgtg ttcgtcgata ccgtgttgcg 17160
cggctggaaa aacgttcagg gcgaagacgg caaggaaatt gcgtattcga aagacgccgc 17220
aatttcgctt ctttccgaac tgccggacgt ttacgaacgt ttgcaggaag aagccaagtt 17280
ggcttcgaac ttccgcgata acgctttgga agccgaagca aaaaactaac cgaagttttg 17340
gcgcacctgt tggaacttgg cccacacgaa caggcgatag caaaacaagc gatgcgcgcg 17400
gggcagccgt tacccgaacg catcgcgaac gcgccagaac ttgaagtagg cttgcagttg 17460
tatttgcaag ccttcttcga cctagatagc gaacggtcgc acggcaacgg tttaacgccg 17520
ataccttgga caagtatggc ggcttacgca agggctttcg agtttgacga agaacaaacc 17580
gaagacttgt tttactttat gcgaaagctt gattcggaac acttgaagaa actagcggat 17640
aaacagaaag cggcggcatc gaatggcaaa aagcctgcta gacctagccg ataggctgga 17700
aaagaaagcg aaggcaatag acgaagcggc gtcacaaaac gccgtcgata ccgctttggc 17760
tattgtgggc gacttggcgt acaaaacgcc cgtagatact tcgcaagctt tgtcgaactg 17820
gattgtaacg ctagaaagtc cgtcgggtca acaaatcaag ccgcattttc cgggttcgca 17880
gggttcaacg caacgcgctt cggcggctga aacgttgaat tctgcgaagc ttgttttacg 17940
aaacaagaag cccggccaag cgatatttat tacgaacaac cttccgtata tccgcaggct 18000
gaacgacggt tattcggcac aagccccggc ggggttcgtc gaacgcgccg ttttgattgg 18060
tcgcaaaatg cgtaagaagt tcaagattaa ggattaaaag aaatggccga cgaaaacata 18120
gaaattaagg ttcaagacaa agtttcgccg tccatttcga cgaagcttcg cacaattgca 18180
agcgaagccc gcaacgccga cgccgcagtt aagaaccttc aaacgcagct ttcggcaatt 18240
aacgtcggcg gcttgtcgca gcttatcaac gcatcggcca gcgcaacgcg ccagcttcaa 18300
caaggcgcgc ttgccgcgca acggctggca accgaacagc agcgcaccgc aacagccgca 18360
gcgcaggccg ccgcagccca acagcgcgtc gcgacggctg cgacccaagg ggcaaccgcg 18420
caagccaacc ttgccagcgc cacgcagcgc acgcagacgg cccaacagca gacggcgaca 18480
gccgcgcagc gcctagcgac agaacagcag cggacagccg ttcagacggc caacgcggcg 18540
gcagcgaacg accgggccgc ccttgccgcc ctgcggctgc aacaggcgca agaccgggcc 18600
gccaattcga cccgcaatgc gacttcggcc cttggcggat acattcgaac cgctgccgga 18660
attcttggcg taacgatttc ggcgaacgca attcttgcaa gcgccgacgc atacgttacg 18720
cttcaaaaca agttgcagaa cgtaacgaag tcgcaagaac aagttaacac gcttacgaac 18780
gaacttttcg aactgtcgaa ccgcacgcgc gcgggtattg aagaaacggc gacagcgttt 18840
acacgcttcg accgtgcctt ggcgtttatg ggtaagtcgc aagaagattc gttgcgaatg 18900
accgaaacca ttaacaaggc tttgattgtt tccggtgcaa cagcgcaaga agcgtcgtcg 18960
gctttgttgc agctttcgca gggcttcaac gccggtaagc tgcaaggcga cgaattccgc 19020
gccgtttcgg aaaatatgcc aatcgttttg gacgcagtag cgaaggcgct taacgttccg 19080
attaaccgcg ttaaagaact ttcgaccgaa ggtaagatta cttccgaagt tctgtttgaa 19140
gctttcaagc ttatacaaga ccaagtagac gcaacgttta acaagacgac gcccacaatc 19200
ggccaaagct tgacggttct tcgcaacaat gcaattcagt tcttcggcga attgaacaaa 19260
gcgaccggct ttactgccgg gctttcgcgc gcgattattt ggcttggcga aaacttgaag 19320
acggttgccg taattgtcgc cgggcttggc gtcgcattgc ttgtcgcctt cggtgccccg 19380
ttggttggtg cgctggccgg tgcgacggct gcggtaaaag cctttacgct ggcgcttgct 19440
tcgaacccga ttggccttat cgtcgtcgcg ctttcgaccg cgattgcgta tctaacgctt 19500
ttccgcgacg aaattaactt gggaatcgac gacgttacga cgttgggcga tttcttccgc 19560
gcgacctttg aaggcatcgg ccaagcaatc gacgacgtta aaaccatcgc gtcgcaattg 19620
tgggcagaac ttagcgactt cgcaagcgca gcccttggcg aaattacttc gaacgttgac 19680
gattcgactt cttcttggtc ggacagttac gccgaatttt tccaaaccaa ccgtacaggt 19740
tgggccggtg cgttggaaaa cgccgcaaaa gttcttgacg caatcgccgg gcttttgacc 19800
ggggccgcaa cgttcgcagg ccgtgcaatg gccgaagttg ttatttcggt tcaaaacgga 19860
atcgcaaacg cttacaacgt tgtcgcgggt tggattgaaa acgtaacgaa taaagccatt 19920
gaagccgcga acaggcttcg cgcgatggtt ggtaagtcgg cttacgaact tgtccaattc 19980
gaacgcatgg gcagcgcagg ccaaaccgaa ttcgaatctt ggggcaagct ttgggcgcaa 20040
tcgctggaag acggatttaa cagccaaggc ggcgcaatgc aaaacctgtt gaacggcctt 20100
ttcgaccgtt cgcagcaaat cggcgcgcag cgcagggccg caggaagcgc gcagcttcgc 20160
ggggccggta cgtcgcagct tgccggggct accgacgcaa acgcggcgaa ggctgcggaa 20220
cgtcgcgcgc tggcgttgga aaaaatcaat acgcagcttg ataacgaatt ggcgcgcatg 20280
ttccagcttc aaccgcagcg cgaagcgcaa gcgaagttcg accaaatcga agaaagcttg 20340
attcagaaaa agattaagct tacgtcggaa gaagccgaag caatcaaggc gaagattaag 20400
gccgttcaag acgcgaccga agttcaacgc cagttcgacg caatttacgc cgaagctgtt 20460
aaccctttga aggagtacaa cgcggcgcag gaagcggcga acaagcttct tcaaatgggc 20520
gcgattacgc aggaacagca cgcccgcgca gtaacgaagg cgtcggaagc ttacgcgaat 20580
tcgcaagacc cgttgcggca gtataaccgc gaccttgaac agcaattgca gcttttgcaa 20640
atgctgccga agcaacgcga aatcgaacag caggttatgc aagttcagaa cgatttgctt 20700
gcaaagggca tcgttctaaa cgaaaccgaa ttggcgcagc ttcgcgaaaa gctgttgctt 20760
attcagcaag taaacgccgt ttcgcagcaa gaagcgtcgt tgttggacgc cagcgtaaac 20820
aagcgccaac agtttatcga ccaacttaaa gcaattcaga acttgcgcaa caacagcgga 20880
agcggtttca acgcaggcga ccaagccgaa gcaactaaca gcatgttgca aggcatgggc 20940
attgatacga cgaactttca aacgcagctt aacgcgcaat tggcgcaata tcaaacttac 21000
gtcgaacagc ttaaaatgct gaacgaacag cgtttgatta gcgaccaaga atacgccgcc 21060
gcaagtatgc aacttgaatt gcagcggcaa aacctgtact tgaattcggc aagtagtttc 21120
ttcggaaact tggccgcgct tcaacagtcg ggaaataaga aaatggcggc ggtcgggaag 21180
gccgcagcaa tcgcgcaagc gatgattaat acgtatcaat cggcaacgtc ggcttatgct 21240
gcgatggcgt cgattcctta cgtcggcccg gcgttgggcg cggctgcggc tgcggctgca 21300
attgccgcag gcttggcgaa cgttcagcaa atccggtcgc agaataccgg gtttaagtcg 21360
ggcggtttta ccgggtcaat gggcgtaaac gaagtcgcgg gcgtcgtaca cggccaagaa 21420
ttcgttatga acgcagcggc gaccaaccgt attggcgttg cagaccttca agccctgcaa 21480
acgggcgcgg ccagtgttca gcgcaacgac gaacaggcaa tggcggctag tgtgggcagg 21540
ggcggcaatt ccgaacccgc gcccgcgccg gttgtcaatg taccttttag cgccgttgtc 21600
gtacaatcga aagaagcggc cttggccggg cttaaatctg cggaaggtcg ggctttcatt 21660
ctggaaacta tcgaacaaaa cggcggcacc gtcgcaaaaa tcgtcggagt taaataaatg 21720
ggttacgcaa ttggaacagt tacaaaaggc ggcggcgacg attgccatta tcaagttttg 21780
gcaattatca aaacgctggc cgaagcgaac ggatggacga cgcttcgtta cgtatcaacc 21840
ggacttgacc gcgaattgat tttgaaaggc gttggtttgt ccggtcttga agaaatcttt 21900
gtagggttta agtgttatca aagcgtcggc ggcgattatt acaatattaa ttgcggaact 21960
ttcgtcggtt acgtaagcgg aaacactttc gaaacgcaac ccggcgcgca gtttagcgca 22020
acgcctgccc acaataacgc gattacgtat ttcataacgg ccaacgccca acgaatcgtc 22080
ggatgcttca aagtcggtac gccggtttac gaacattttt acgtcggcaa gttctttccg 22140
tatgcgcggc ccggtgaata tccgtcgccg cttgtttgcg gtgcgatgct ttccaacgcg 22200
caggcaatgc gattcgacaa caacgcgcag tcgttcccgt atccgggcta ttattacgac 22260
acgacgaaca accgccttta tattcgcgac caagcgggca actggattcg cccttggtgt 22320
tggccattta ccaatggaaa cggcgcaaac accgcgcttg cagggccgca aggaacttcg 22380
acgcttgttc ctgccgacgt gtattaccaa cttgaaccaa ttattttggc gcagcaaagc 22440
aacaataaca acccttcgaa cgtatggggc gaattcgacg gcgtttattt ctgttccggc 22500
tttaacaacg gcgtcgaaaa cgtagttcag gaaggcggaa gttctgtcgt tgaccaaacc 22560
gggcttaccg tacttcaagc cgtagacgct attaaggcgg tcggcgggcg ggcgtttgta 22620
atgttgcaaa acgtctatcg tacttcttgg cgcgatttta tcgctttgga aatgaaataa 22680
tggcatacat tacaggaacc gccgcaaact ttgcggattt gaaaacagcc attgaaaacg 22740
cctgtacttc taacggttgg gcgcttgccg acggcatatt gtcgaagaac ggttgtttct 22800
ttcaactaat cgccgatagt ttgggcggtt atccgcaact tcgcttgcat ggcggaacag 22860
gcaaaagcgg ttcgacgctt acaggccagc cgcctagcaa ttacggggca aaggtcgcaa 22920
gccctgtcgg aaatcaaatc gtattcccga ttaattacga aatacatttg tttaccgacc 22980
ccgacgaagt ttattgtatc attaattaca attcggactt ttaccaacaa ctttcgttcg 23040
gcaagtctga tattccgggc attggtggaa ctggcgcatg gtttaccggg gcatatcggc 23100
acgactacgg attgaccgac gcaacttacg cccatagggt ttttacggcg atttcttggc 23160
ccgaaataac tggcgtcgca acttatgccg gaatgtgcgt cggtttgttt tttgaaggca 23220
acgcttcgca aactggcgga agttatttcg caagcttcgt acataccggg cttgattcga 23280
cgccttggcg aagcggccac ggcggcgaca cgctgttaag cggctatcgc ggggccggtc 23340
tttccgtcgg ttcgctttta atgtcactgc cgaatatttc gaaccaagca acgattttgc 23400
ttcctattaa gccggttgcg cgtcgttcaa gcggcggcgc aactattatc gcaaacccga 23460
aaaacgcgcg ttatttgcga atcgacaacg ttgtaccggg cgaaattatt acattcggtg 23520
ccgaccaatg gaaggtttac ccgttttacc gcaaagacgc aaccgttcga aacggcgtcg 23580
gttggtcaag cggcgcgacg catagcggaa cgttcggata cgccattaaa tacactggaa 23640
cttaaaaaga atggccggaa gaattggaac atttttagcg gcggaaagtt ttatttacgg 23700
cgattataac ccgaaccttt ccgccgaact taacgaactt accgccgcag aatacgcgcc 23760
gacgatttac gacccgcgcg atatgttctt gcgcggcgga tattcggcgt acaaaatcgc 23820
gccttcgaat cgctggccta ttgtgggcaa agaacaaggc aacttcgggc gcgactatta 23880
ttatcgcgtt cacgtttcgc cattggtctt ggacttgcaa acggtcgcgt cgtcgcaaac 23940
gcgcgaattc aacgtttgga acgcatggcc cgaccgtgcg gctaatcttg acgacatttt 24000
ggtaagtaat ccggtcggta ttgaaattac aggccaagcg acgccgtatg caatgccgcc 24060
gcttcaagaa cttacttacg aaataaccgt aggcgtttcg gggccgccga atattaacgt 24120
tgaagttcaa tttgactttt ccaacgttcc cgacccgctg ccgattctga ttacaggaac 24180
gcgcgcggtt aagttcgaca ttgtgcccga agttccattg aacgaaactt gggaatggtt 24240
aagcgacaac attgtagcgg ttgacggaac agaacagcgc atagcacttc gcggcgaaat 24300
gccgcgtatt gaagaacgct tgaaagtaat ttttgattct gtcgaagccg tgcgccgttt 24360
ttacggcgac gttatgtcgg cagttggtcg gctttggatt cccgaatttc agtacgcgac 24420
ccgaattact gccgcaagca atagcggcgc gcttcaaatt tattttgacc gaacgaagac 24480
cgacgttcgc gataccgaat acgtcttgat tcaaacgccg cttgttgcgg cacttgtcga 24540
aatcgacgta ttgacagccg acggggctac gcttggttcg ccgctgttgt tcgacattcc 24600
ggcagggtcg ttaatcatgc cgggttcgcc cgcgcttatt gacgacaata cagcacttgc 24660
gcggtattcc gtcgataccg tcggcgaagt agaaattaac gcgaagatga ttcgccagcg 24720
ttcgcaactt acccgacccg gcgcaaccgt cgtacttgaa cagttcggcg gcgtgccgat 24780
tttgacgaag cgtccgcttg cgaacgacct tgtacgcgac gaagtttcaa ccggccaaat 24840
ttcaatcgac aatcaaaccg gacttgccga cattattagc cgttgggact atagccgcat 24900
cggcgggccg cgaagcttca aagtaaaccg tatttcgaat cccgaagaat tggattattg 24960
gaaagcgatt cttgcgtatg cacgcggaca ggcgcgcaag ttttggatgc ccacatatcg 25020
aaccgatttg gctttggccg tgcaaccgtc cgacgcgact tcaagctata ccgtcgaagg 25080
gtcggagtat gccgaaaaga tttggccgat tatcacgcat cgttatatcg aaatcgaaac 25140
agcgtccggc attcatcgaa ccgaaatatt gggcgcaact ttgcttgaaa ctggcaatac 25200
aattcttctt cttggtacgc cgcttccgac cggggccgga tggatggaag ttaagcgaat 25260
ttcgtacttg ttgcctgtac gccttggcga cgataaagta gaatggaagc attacggcct 25320
tgaatcaatc ttgaatcttt caattcgaac ggcggaaccc taatgtctga ttacgacgac 25380
aaagaagaaa gcgtaggcga tagcgcgcca tttgaacttt acgaattcgt cggttcatat 25440
cgcaattatt acatgacttc cgacgttctg gcgcacgtct tcaacagtgc gacatataac 25500
cccgttccgg gtttgaagcg cagtacgtta aaagtcggaa cgcacgaaga cgacaacgta 25560
gatatgacgg ttacgcttcc gattacggaa caaatcgtta aagactatgc gtttcaaact 25620
acgccgccgt ctttggtttt gacgatttac cgtcgccagc gcgacgccgc aacttgggtt 25680
gcatactgga aagggccgat tgcttcaatt tcggttgacg acgaatttgt aacgtttaga 25740
acgccaagca agttcggttc gatgctgtcg ggcaatatac cgaacgttta cgtacagccg 25800
ccttgcaata acgttttgtt cgacgaacgt tgtaaggtaa gtcgcgtaag caattcgcta 25860
gatacgcagg tttcggcggt cgaaggtcgt aacatttcga ttccgtcgct tggcggtttt 25920
cccgacggtt ggtttatcgg cggcgaaatc gcaatacctg cgcgcaacga acgtcgaatg 25980
attgtcgcgc aagccggggc aattctaacg gtaaattacg aattcgcgcg tatttctgtc 26040
ggcacttcgg ttcaggttac ggcaggttgc gaccattctt acaacggggc caacggttgc 26100
cccaagttca acaatcaacc gaacttcggc ggttgcccgt ttgttccggg cgaatcgaat 26160
aacgtatttc aaagcggggt tcattaacaa atgtggattg caattgtctt cgtcgtcgcc 26220
ttcttactta tggcggcgct tatgccgaaa ccgaacgtcg aaaacgcacg cgcggcgaaa 26280
cttggcgact tccaatttcc gcgttctaag tacggcgacc caatgccgct agtgtgggga 26340
acagtgcgcc agaaatcgcc gataacggct tggtttggcg acttccgccc ggtgccaatt 26400
cgtaagaaaa tgaagaccgg gctttttagt tcgaagaaag ttacggtcgg ttataaaaat 26460
tatcttggca ttgattgcat tttgtgcctt ggccccggcg ttcggcttcg taagttttgg 26520
gccggtactt accttgtttg ggaaggcaac ttaacttcgt ccggcgatat ttacattaac 26580
aagccgaact tgtttggcgg cgaagatgaa cgcggcggcc tgcaaggtac aatttcgttt 26640
tacgacggtc gttttgaccc gccgcaagat tcttacttgg tttcgaagat tggcccgaat 26700
gttccggctt acaacggctt tgcgcgtgcg ttgttcaaag ccttttatat cggaacaggt 26760
acgacgccgg aaccgttcag cttcgaaatt cagcgcatta cgtccggctt gcacgcgact 26820
tattcaatca tgccaaacgg gttggacgtt aacccgatgg aaattgttta cgacgcaatg 26880
acgcaaaaat ggggccgatt cggcaacctt gaaagcgaaa ttgatatgcc ttcgttcacg 26940
gcctgcgcgc aaacgctgta taacgaaggc ttgggtatgt cgcttattgt tcaatctgcg 27000
attaccggaa aagacttgtt ggaagaagta atgcgcgtcg ccgacggcgt gttgtatcaa 27060
gacccggcaa cggcgaagat tgttgcgaaa cttgttcgcc aagattacac aatttccgaa 27120
cttcttgttc ttgacgaatc ttccgttaag gaacttcgca atttccagaa aacaacatgg 27180
gaaaacacct ttaaccaatg ccgggttacg ttcaaaaacc gcgccagtag ttacgacgac 27240
agcgtagcaa ttacccaaga cttcgcaaat atcaactttc aaaaccgcgt taagtctacg 27300
gaagtttcga cgcccggcgt taccgacgca acggtagcaa gcaagattgc ggcgcgtcaa 27360
ctttcaattt tgaacgttcc gctttacaag tgcgacttga ccgttaaccg caaggcgcag 27420
aacttgcggc ccggaagcgt gttcgttctg aattggtcgc cgttcggcat ttcgaatatg 27480
gttatgcgtg ttacgaaaat tgatttcggc gaacttacgt ctaaccaaat caagctttct 27540
tgcgttcaag accgcttttc gtcttcaacg cttacgttcg caccgcccga aggttcagga 27600
tggacgccaa ttagtacgga accgacgccg gttacaacgc ggcttctttt tacgccgcct 27660
gcgttcctta cgtcaaacag cgaaaacgaa acactttcca attttgataa ctacggtcgg 27720
ttgtatcttg ccgcagttgc gccgggttct acgtctattt cttacgacgc aatgacggcc 27780
ccggacaatt tcagttcgtc gcctattctg tcgcttgaaa gtgccccgta caacggcggc 27840
ggcgtgttgc tgaacgccta cgcttcgaca gtcgccagcg aaacccgtaa cgatacaagc 27900
ggaagccttg tcgtaaccgg cgtgtcgcaa gcaagcattg aaggtttgca gcaatacacg 27960
acgctagacc aagcgcgcga cggttcggcc ttcctgctga ttaataacga actgttcgtt 28020
tatgtgggct tcgttgacaa tggcggcggt caagttactt tcccgaacgt gtatcgcggc 28080
gttctggata ccgtgccggg caatcatgcg gcgaacgacc gcgtttggtt cattagcgga 28140
accgacggac tgttgccgga acttgtttcg gtcggaacga cgcgatacgt taagcttttg 28200
gatacgactt cgggcggcaa gcttgatatt tcgttggccc cgtcgtttag cgggtcgctt 28260
accaaccggg ccgggttgcc gctgccgccg cagtacctaa cgcttgcagg aagccgcacg 28320
ccaagcccac aagtaggcgc aacgtcgatt gttgcggaat ggcgcaaccg cagccgggcc 28380
gataccttgt tgcgcgtgta tcacgacacg gcgaacacgc gggaagctgg caccgaaacc 28440
cgcgttcgct ggcgtgtagg cggcggcgct tacacgaccg ttacgactgc cggaagttca 28500
acaacccttg acgtaacggg caagtacggc acgctagaag ttattgtaga ttcgttcatt 28560
acgtcgaatt cgaaatattc gacgtacagc gattcgctta caatgaccct aacggcaccg 28620
ccgccgccgt aatcaaaaga acgacagcgc ggcggttttg gccttcttca accgcccgca 28680
gtcgtacaac atttcgaccg cttcgttaat gtaccaatcg taatttatat cgtcgggaaa 28740
ttccgtcggc aagtccatta cagggcgcgc gccgtcggtt ttcccgactt tgtttccgct 28800
ggatacgtaa gcaatatatc cggcttcgtt cttcgggtaa taccaacgaa cgaccttgcc 28860
aagataacga ccgttctttt cgccgccgcc gcgcacgttc tttactgcaa cgaagcgccg 28920
gaagtcgcgg cattccttaa ttgtcttttc gaccggcgtt ccgtcaacaa ggaaccgaac 28980
aacggcgtcg gaacaaatca gggtttcggg gttcttcgaa agaatcgaat tcaaagccga 29040
cccgcgttcg caataagcgc ccttcgtttt tgcgcccaac tgttcgtcaa taaagcgcgc 29100
ttcggggtcg ccgccgtctt ccttgattgc gacataacta ttaacgtcgc ggctgaatac 29160
ggctttatat cgcgtttctt cggtcttgta tcctgtccat tcttcccaag acgcaataag 29220
cgcccttact tcgtcgtgtc gcgatttgtg atacttggaa ataatgccgt cggtattgcc 29280
ggaaataact tcgatgccga tttgttccaa agcttcgata agcataagca acacaagttg 29340
cccggtaatc gttacttgca acataagttg cggcgcgtaa agcgtcgaat acttgttgcc 29400
aagcttgccg aagcttccgt taatggtaat tttcaaactg tcggcaattg tcttccattt 29460
cttcgcggct gcgcggtcgc ccgccttctt tgcctttgcc gcttcggctt tggcgtgaat 29520
tcgcgtattt acgatttttt cgtaaacggt caagaaagct tcgcccaagt gcggcgggca 29580
aagtctttga ttaaggattg ttcgcgggta aaacgattct acgtcgttgt ctgccaaaat 29640
tatttcgtcg gtcgcatagt gcgccgtttt cttttcggta gaatgcaaac cgcccatgcc 29700
tagtttgtac gtcgaaccgc caatggttac tttcagcttt tccagttcgg gcggcatgat 29760
tggcgacccc aagccgtcaa gatagaagcg ggcatttcgc acgacttcca gcatttcgcg 29820
aagctgcgtc gtttgatagc aaacgaagtc gggcacgtcg taaaccaaga ccgtatcggc 29880
ggatagtgtg ggcttcttcg gataatagcc aaggactttt tgaagttcgg agttaataac 29940
cgcttcggct acttgtgcgt ctgatttcga ccgaaggtct acgccgtatt cttccgacat 30000
ttcggcccgc aacttcaatt cgggcgcaag ttcgttaaaa agaagttcgg tattcgaaag 30060
gtcgttgcag caataaggcc gcactatttc ggcgtcttcc tgcgttaata catggtcttc 30120
gggaaacggc aagtcttgca tacgttcgca atgcaagcgc cccgcgtaca gcttcaacga 30180
accttgaagc ggcgcaactt ctataaggtc gatatgattg tatcggccaa tttgtacgcc 30240
gtacttcttt tcgaacgcga acggcgttac cttttcggtt ccatagttcg gcccgctttt 30300
aatgatgaaa tcggacgctt ctttaagctt cgcgcagctt gccccgcgtg cggctagttc 30360
aatcatcggc aagtcataat tacgcgaatt gaagccgacg atacagaacc gccaaagcat 30420
ccaaagaagc ttcgtcgggt taaaatcatg gtcggggctg cgttcgaacg cgacgaactt 30480
tccgttatca agcgacttaa acgctacgta aaagaaattt acgtaagttt caacgtcgaa 30540
gacgaagaca cttccggcag gcattgccgt tagttcttcg tccgacataa attcgacagg 30600
ccgcaaagcc tgtcgaatgc ttgcggccag cttatcgacg acgcggcttt tctttgcgac 30660
gataaagcct tgttcgttaa tcatgcgaaa gccccttaaa acggtatgtc gtcttcgtaa 30720
ttgtccgacg gattatgcgt cgcttcgctg ccaaggtcaa gacccataag aacgccgcgc 30780
aaattgtcgc cgaaaaatac gaccttgttt gcttccggtg cgaaatgcgc tttcttaaac 30840
gcatgttcga cggaaagaag caacttcgca gaaaagccca ttcgttccgg caagccttcg 30900
attttgtacg tcgaagcttc ttctttgtgc atcttcgaca aaaccgcgcc gtcgtcgaag 30960
aatacgtttc cgttcgggct gaatgactga atcgcgcgaa cgccgttgta aaaatcgtcg 31020
ggaaccggcc aagggttcaa accgtcaact tcgaaaacga cttggtaatt cgggtaacgt 31080
tcgccgtaaa gctgcgtttt gataaacgcc ccgttttcga aatagaacgt tgccgacgac 31140
gccgaaaagc cgaagccggt aagggccggg ccagccttgg cgacagcgac cgccgcgcac 31200
ttcggaagca tcatgccggg cggaaggtcg ataccgtgcc acgcttccag caaggccgcc 31260
ccgttggtcg cgacggcgct tccggcttgc agcaagacgg cggcataggt cgcattcggt 31320
gcgccgtctg tcgcaagccc tgcgaccgca gcgaaggccg ttttaatccg gtcgtcgatt 31380
gtggcgcatt gcgggtcggg cgcggtaatc ggcacgtcgt cgaagcccac acaaggcacc 31440
aacgcccgga acgcgccaga cgatacggca agggcgttcg cggtaagctg cgtaatcgac 31500
agttcttcgc cgaccttcga cagcgcgtcg ataaactgca acgtatgcgg gcacgctacc 31560
aagtcttctt cgataggatg cgcgacggtc aaaaccccgt cggaagccgc cgcccaattg 31620
tgggcaatat gcccgaactg aacgttcgtc ggcccggctt ttttctgcgc gatggcaacg 31680
aacttcaacg ccgccaaaag cgacgccgca gggcttggcc ccttcgcctt tttctttccg 31740
gctgcggctt gcctgcgcgc ccttggcttt ttcttcggct gttcggcggc tggcgcttcg 31800
tcttcgggcg gaagcggaat attactgtcg attttcattt tcggcccctt cttcgtcaag 31860
tttcgaaatt gccgcttgaa gatagttcgc taagtctagc gtttcttcta gcgcgtgttg 31920
caaccaatcg cgaagcgata acgggttatt atccgtcgtt acgccgtact ttgcaagtcc 31980
aacgattgaa cgttgcaaca gtaattcgcg gttcttttct acgtttttat cgggcgatgt 32040
ttcggccatt tgttcaccat tcagcggaaa gaacttcggg atactttttg ttcacccata 32100
cgcgaacacg gcgcggcgtg cgaagttctg aaacacgctg caaagcttga taagtcgtcg 32160
gcggcggttc ttcggcgtgc cgctgccgcc accaatcgcg cgaacgtttc gcagcaagcc 32220
ccggatgttc aagcattata aattcgttga acatttggaa gccgcaaaag taactaacct 32280
taatcatcgg cggtttcgtt agatttccgt ttgcgtcgcg cttttcgtgc aaattgtaaa 32340
gtaccttttg aacgtcgaaa tattcgacta tcggcgcgtc gcttcgcagc ggttccagcg 32400
ttccggcagt tgcaaacaac ttcgtttcaa aagtaaattc ggtgccgcag ttgatacact 32460
gccgggccgc agcgtgatta tatacgccgc atacgtcgca aatacgaacg ggcgcgtcgc 32520
cgccgccttt gcccggtctg ttcggaattc gcgggtcgtt gattgggcca agccttcgaa 32580
cgtttccggc aaagtccgcg tacaggcaat tttgtttgcc agtatccggc gacggtcggg 32640
ttccgcgtcc caacttttga acgtgtttgc ccggcgatag cgtcgggttc aaatcgccga 32700
taaagtcgat tggcggatgg tcgaaacccg ttgtaagttt ctgccctgat accaagccgc 32760
gaagttcgcc agctttgaaa gcgcgcaagc gttcggtatt ggtcttcgaa ggcaacttgg 32820
aatgaaccgg caaaacttcc aagccgtaag actgaattac gttcgcgacg tgttcggtat 32880
tatcaatgcc agttgcgaag ataagccaag tcgaacggtc gtaagctatt tcggtcattt 32940
cccgaacagc gttaaaaaca acttcgtcgg tatctacggc ggcttctagt tgcttgctgt 33000
tgaattcgcc agccgttacg cctactttcg acaagtcgat ttcggttcgc gtcggccttg 33060
cgataagcgg cgacaagtag ccttcggcga taagtcggtt aaacgattcg acgccggtta 33120
tatcgtaaca aatgtcggta aaaattccgt tgtcggtaat aagccccatt ttcattcgat 33180
agggcgttgc cgtaaatcca ataactttaa gatgcgggtt aatcttgcga agttcggcga 33240
taatgtattg gtaatgcgaa tcttccttgt cgcttaacaa atggcattcg tcgattaaca 33300
gcaagtcgcg ccaaccgaaa tggcgaagat gcgccgggcg tccgtcgtct tcttcaagcc 33360
cgcgtttaat cgcaggcgct acggactgaa cgccgccgaa tacgattggc ataatcatat 33420
cgcgggaatt caagcccgcc gaataaatgc ccattggcgc ggtcggccaa atcgacataa 33480
gcttttcggc gttctgttcg attagttctt taacgtgcgt aagcatcatt attcgttgat 33540
tcggccagta accgaaaatt cgacgaataa agtttgcaat tacgaccgac tttccggttc 33600
ctgtcggcat ggcgacgacg gggtttccga ccccgccgcg ctggaaataa tcgaatattg 33660
aatattccgc ttcgtcttga taccaacgcg gaacataaat attagacatt ctttgcaacc 33720
gctgtaattg gcgaataaga atcgcaaccc gtagcgatga aatcgcccgg aattacgccg 33780
ttgtgaactt cgcaaaacca ttcgccgttt tcgacaggat ttgcggcctt gcagcttcgg 33840
cagttctttt caggaatcgc gcccttgtgg caaatatcct tcgccgcgca atatccgcac 33900
ttgtgaaacg tcggattatc cgacaagcgc ggcggcgctt cctgcgacag aatgatttgt 33960
tcggccttct ccttcatttg ctggcctagc cgatggtcta gctttacaac ttcgcaatgc 34020
aagtcgtcgt cgttcttgtt aatgttcagg taaagaacat aacgaaaacg atattcgttg 34080
ccatacgtcg aagtttgggc gaaatgctgc ggcttgctta ctgccattcc ttgcgacatt 34140
aagtccgaaa agcctttgcc ggttccgttg gtcttgaatt ccagcaaaac gggttcgtcg 34200
ataccgtagc gttcgggaag ccgggcgatg ccgtcaagcg acccgccgaa gtgccccata 34260
acgccggaaa tgcggtattg tgggaattcc aagccgtcgg ctttcgcgcg tgcgacgtgt 34320
tgggcgtaat acggattttc gttcgtaata aggtcgcagc ccaacggcag ttcttcgcct 34380
tccttcaata tgccgtaact gtcgctttcg gcttggtaaa agaaaccgtc gcggttttcg 34440
taccaacatt gaacgccgat gccttccagc cattcgataa agcgggcttc ttcacggtgc 34500
ccacgattaa acaagcgttg cttgcgcccg tcggtttgtt cgcggaaaca ccaacggaag 34560
atataccaaa gcttccgctt gcattcgtcg ccaatcaaag acgcgccaag gtgccgacgg 34620
tgcccgccgt catacgtgcg aacgcaatat tcgtctatat cttctttaat gcgcttcgca 34680
agggcggttg caacgccggg cgcgtcaagg tctacgcttt gttttcctgc gtcggatttc 34740
gtcggctgcg cgtcgttcag cgttaattgt gcgttcttcg cgtctttgct gttttgcatc 34800
gttcttcacc ttgcgccgca taagggcttc gtaattgttc gctagataat ccgccgaaag 34860
tgtcgcaacg tctttaagtt ggtctttcga cagccaagat atatggcatt ccgacgggtc 34920
gattcctaat tggttcgcaa gccaattgta cgccttcgcg cgcgacatta agccggtttg 34980
ccaaaggcgg tcgaattcgt tatgggcttt cgttcgaagc tggcgcgttg ttctgtcggc 35040
catacgaccc aacggaataa acgtgcccgg atggcaacca acggcggcgc gacaatcatt 35100
gcaataataa atgtgcggcc aattgccata agttcgcccg taaatcttgt cgttcgttgt 35160
tagttcaatg ttgaacgaac aacaagtatc gcattgttcg ggcgtcggca atgcgtcttt 35220
tattttcgac ataagttcgc atactcctta aaaagaaacg ccggggccgg ttaagacccc 35280
ggcgaatcta cgccgcgacg gcgttacgcc ttaacgctgg ccccaaggtg ccgcgcctgc 35340
cggtgcgccg ccctgcgcgg gctggccgcc gccctgttgc cagccgccgc cctgcggttg 35400
ctgctgcgcg ggctgttggg caggctggcc gccccatgcc ggttggccgc cctgcggggc 35460
cgggttctgc gccggttggc cgccccaagc tgcgccgccc tgcggctgtt gggccggttg 35520
ctgcgcgggc tgctgctgtc cgccccaagc ggcaccgcct gcgggctgct gcggctggcc 35580
ctgcggggca ccgccccaag cgccgttcgg ctggccctgc ggctgctgtt gctgcggctg 35640
cgctgccgct gcgcctgcgc cagccttgcc cggttcgttc ccgttcatat cgaagacctt 35700
tttaacttcg gtatactgcg ggtcgttttt ctgcgggccg acttcgataa gaaacggcag 35760
gttgtgaagc tgcgacgaat cggtaacttg gaacacgccg attacgtggc agattgccga 35820
aagctggcga tgcgcgattt cgacggtctg ttggttggaa tggtacaggt tcaggcgata 35880
agcgccggtc gtgccctgct gcgggccgtc gataatccgc aagttcagtt gcagataacc 35940
gccgtcgttc gccttgttgg ccttgacttc ggaagattcg acgataaccg gatgcttgcc 36000
aatcggcagg cttccgacgc cttgggtcgg gtcgtactgt tgggcgttga acgcttggat 36060
aagttgcatg atgtttcacc ttttcgaaaa gtcttcgcca ttggttacgg cggcgaagta 36120
aaccgtttta ttgcatcgct ttcgcgaaca atgcggtaag gtcgggctgt tcaagttcgt 36180
taagattgcc caaacggtcg cgcgcgaaaa cttcgggaat ttccttcgtt cgcagcgccc 36240
gaaccggctt cggcattccg ggtacgcttg cttcgcccaa gtgcataacg ttatcgaaca 36300
aatgcggaac tttaacgtta aggtctttgc ccggaaagaa cgggcgtttt tgcataatcg 36360
gttcgtaagt aacttcgccg ttctgcaaaa tcgtttgccg tccgttttcg acaagggctt 36420
gctttgcaat cattacgata tgcttttgcg gcatgtaata caagtcgttc gcgattttca 36480
ttacacgttc ggacatattg ccgtaagcct tcataccgtg tttaaccttg cccaattcgt 36540
cggccaagat aatttcggcg atgttcgaaa tactgtcaat acccaacgta tcgaaattcg 36600
cagcttcgcg cgatttcata aaccattcga agaattcgac gataagcgcg ggcgaatacg 36660
cttcccatgc gggaacgttc gaaccgcgca tggacagcat accgggttcg gtcacaagca 36720
aaaccggacg cggcgcagta ttgataagcg gcgttttgcc ggaaccgggc gcaccgaata 36780
cgacggactt tacgccgtag cggcgggcca gttgcgacgc cggttttaat tgcgacattt 36840
gcataacttg caccttcggg aatggccccg gcgcttgtgg gcaaccgggg cggggttgtt 36900
acttcgactt cggcgcgaca agttcaagcg cgggcgttgc ttccttcgac gttacaactt 36960
cgtcgataag cttacggaac ttgtcgggaa ggttcttgta ttcggtaacg gaaagttcgg 37020
gcttccattt taccaaacgt tcggcgataa attcgccttc ggggccggtc ttttcgattt 37080
tctgcaaagc cttttcgacg gcttcttggc cgcctgcgaa gctgcggtta atcttaaaaa 37140
cggctttcag cttgtaacct gcgccaagtt cgacgttttc ggttccttcg cgcaaagctt 37200
cggggtcgaa agcgaaagcg tttttcagaa cttcggcgcg caatgcggat tcggcttctt 37260
tcgctgcggc aagcgccttc gttgcttcct gccattgcag aatcaaagcg tcgcggttag 37320
cgttttcgtt gaaagacatt gttgttactc cttcggttgg gccgccgcac aattgcgccg 37380
gtatgtacga actatacgac ggcccttcgt tactgtcaag cccctttttc aagaattttt 37440
cgttcttcga acaagtcgcg attgttggcg tcgaattcgg taaaggcgtt cgggaagcga 37500
cgacgaagct tcgcgatgtt gaccgactgc gcttcgccga agttcgaacc aatggcgcgc 37560
agcaaaagcg catcgtacca aaagccgtcg ccgacttctt caagcgcgtt aacagcgtcg 37620
aaagcttcgc cttcggctgc ggcgcttagg gcttccaaca gttcgcccgc ttcggtcgcc 37680
ttgccgataa ttgcatgaat gacgttcacg gctgcggcgt cgtcttcggg gcggtcggaa 37740
atccattcgg gaagcttcgc cagcgttgcc gcgttggctt ccggggccgg aacgccagcg 37800
tcgcgaccgt agaacatggt tttctcgatt gcgtcaagct tgttaagggc ttcgacggct 37860
tcggcgatgg tttcgcggaa gtaggccagc gacacgcggt cgccgtaata cttatccgac 37920
gcggttacgt gcgcttcttc gatatagtcg aaggtctttg cgggttcgtt catacgttca 37980
ccttttagaa gggccgggca ccatgcgcca gcgttccagc actgtaaccg accgcgcctt 38040
tcctgtcaac aacaattgac agcaagcaag aagccgggta agctgcccac aattgctaga 38100
aggtggcgtt atggacattg aagaacttac gaagcgggta aagaaagcaa gcgtcgggcg 38160
gtcgctgcgg gcgctggccg cgcaatgcaa cgtttcgcac gaacttataa gaaagctttt 38220
gcttgccaat tctgcgccga acataacggc gacgacttat aacaagattg ataaaggatt 38280
ggcgaagaat ggactttaac aatattccgc acgaaatgcg gatttacccg caatgggtcg 38340
tttggcgata cgaagacacg gattcaaaga aaccgacgaa ggttccgtat tcagcaaaaa 38400
ccgggcacct tgccagcgta accgacccga acacttgggc cgggttcgac gaatgcgtta 38460
acgctatgtc gtccggttgg tatgccggaa tcggtttcgt attgactgaa aacgacccgt 38520
attcgtttat cgaccttgac gacacaaaag gcgaccaaac ggcgcttgac cgccaaataa 38580
agatttttaa cgaattcaac agctacgccg aacggtcgcc ttccggttcg gggttgcata 38640
tcatcgttaa gggcgcgatt ccgtcgggcc gccgtcgttc gtttatcgaa gtttattctt 38700
cgcttcgtta tatgacaatg accggcgacg tttaccgcaa cgcaccgatt aacgattgta 38760
acgaactgtt aaacgttctt tggggccaaa tggggcaagg ttcggtcgcc gtcgcgcatt 38820
atgccggact tgccgaagcc aaggaaaccg acgaacaggt ttataaccgc gcagtcgccg 38880
cagccaatgg cgacaagttc gccgaactgt acgcgggcaa atgggaaggc atgtacgctt 38940
cgcagtccga agccgacttt gccttggtcg atattatcgc gttttatacg cagaaccggg 39000
cgcagatttc gcgaatgttt cgcgcgtcgg gcttggggca gcgtgacaaa gcaaaacgcg 39060
acgattacgt ttcgtacatg ctgaacaagt gtttcgaccg catgttgccg cccgttgacg 39120
ttgacggatt gcgcaacaag ctagacgaag ccatagcgaa gaaagaagcc gccgacaggg 39180
ccgccgcgtt gtcgcagaac agcgaagcga cgccgcatcc gaaagccccg gccccgaacc 39240
ttagcgaagt ttcgaaggta tacagcgtgc cgcccggatt agtcggagaa attgcgcaat 39300
acatttacgc acaagcgccg cgcccggtgc ccgaaatcgc gttggccggt gcccttggct 39360
tggtcgccgg tattgtgggc agggcgtaca acatttccgg cactggcctt aatcaatacg 39420
ttctgttgtt ggcaccgacc ggaacaggta aagaagccat cgcaagcggc attgataagc 39480
taatggcgca ggttatccga accgttcccg ctgcgtcgga ctttatcggc cccggtgaaa 39540
ttgcgtcggc ccaagcgatt attaagtata tgtcgcgcgg cccaacgtcg ttcgtttcgt 39600
tggtcggcga attcggcatt taccttcaac aaatggcaag cgtaaacgca ccgccgcacc 39660
ttaccgggct tcgtcgcttc ctgttggatg cttacaacaa gtcgggcgaa ggcaaggtac 39720
ttcggccttc gatttattca gacaaggaca agaacacgac cgccgttctt tcgccgtcct 39780
ttaccttgct tggcgaatcg acgcccgaaa agttttacga aggtttgcac gaaggtttga 39840
tttccgaagg tctgttgccg cgttttacga tgattgaata tcacggcgaa cgcccggcgt 39900
tgaatccggg gcacctgtcg gcccaacctt cgttcgaact tatcgacagg ctttcgacct 39960
tgtgcgccca tgcgctaatg ctgaacagcc agcataaggc gattcacgtt caaaccgacg 40020
caaccgcccg cgaactgttc cagcaattcg acgcgcattg cgacgcgaac attaacacaa 40080
gcgaccgcga agttcggcgg cacctttgga accgggcgca cgtaaaggcg ttgaagctgg 40140
ccggaattat cgccgtcggc tgcaaccctt acgacccgac cattaccgcc gacgttgcgt 40200
cttgggcgat taaccttgtc gtcgccgacg ttcgaaactt gcttgcccgg ttcgatgctg 40260
gcgaaattgg catagacaac gacgaaacga aacagcttgc gaaggttatt gcaaccgtta 40320
aagatttcgt cgtttcacct tggccggacg ttgcgaagta cgcaggcgaa ggaatgtcca 40380
accttcattc gaaccgaatt gttccgtaca gctacgttca acgcaggctt gccgctgttg 40440
ccgtctttcg taaagaccgc atcggcgcaa gcggggcaat caagcgcgcc ttgaagaccc 40500
tttgcgaacg cggcgatttg caggaagtaa gccgggccac actggcgaag gattacggaa 40560
caagcgccgt tgcatacatg gtcgcgcatc cgggcgtttt cggcctgtaa cgcgcgcccg 40620
ccagtgtggg cagaacgggc cgccttcggg cggctttttc gtgcctgtaa aaaaaagttc 40680
gtcgtacctg ttgacgtagc ttcgaactgt acgtagaata caaaccatcg aagcaacgaa 40740
acaaaccaag gaaccgaagc catgaacgta accaacctta aaaccgccgt cgaaatcaaa 40800
attgaaattg aattggccgc ttgggccaac ggcgaaaaga aagttaacgt ttccaaccaa 40860
ttcgacgaaa gcgttttgga atcggccaaa gaagcaatta gcgtcgaagc aatcgccgca 40920
atcgttgcca agtatccggg cgcgaacttc gaaggctttt cggttatgac ttgggcgcac 40980
gaactttcgg cgttcaaagc cccggaaatc gaccgggctt ggttcgacta agcaagcgcc 41040
cttcggggcg ttttttttgt tcgtacctat tgacgaagct tcgaagttgc cgtatattta 41100
aaccatcgaa gcaaacaacg aaggactaac gaacatgaac gcacttaaca acattcaacg 41160
ggtcgtaagc tggaacagca agaagaacgc cgacggcacc tttaccgcta ccgcgtacag 41220
cttcgaccat aacgcgccga ccgtcgtaca tgccagctac acgcgcgcca gccgcgccca 41280
agccgtcgca gacgccaaga aggccgttcg ttacctgaaa gcccaacagc gcaccgcgta 41340
aggagtcgcc gccatgtttg cccattactt cgccagcaag aaaacgaagt tcgttgtaat 41400
cagcgaaacg aaccgcccgg aaggccagcg aatcgccgta agcggcaaag ccgaagcccg 41460
cagggtcgcc aaggaacgca acgcgcagcc ttggaacttc taaccccggc cacggccctt 41520
ggcacgcgct aggggccgcg cagcctgccc acattaagcg aaggacgacc gaccatgcgt 41580
acaacccttg cagccgtaaa caaagccctt gccgccgctg gaatcgacgc ggaattggtc 41640
aagggtcgcg ggtactttta cttcgttggt tccgccgtag attgcgccaa agaacaaggc 41700
gtttacggcg tgtatcggct ttccgaattg accgtttccc aatgggtcga agaagcccgc 41760
caacgtatcg aaaaggagta agaacaaatg accgcatatt ttatcgcatt tggcgtcgcg 41820
cttgggttgg cccttgcgcg cttggccgct tcccaattcg aagccgcttg ttacctgttc 41880
tgcaacggcg aatttcgaaa ggcattccgg gcaacgatgc acgcccttac ctgcgttgcg 41940
ttgcttgcgt tggttgtttc gcccgttgct tcgttcttcg atagcttcga agttataagc 42000
attgaacaac cttcgcccaa tccttcgaag ggtcttcgcg tataatagcc gacgggtcgt 42060
ataatgggca cgtataagcc ttaaaccctt gatacataag gctttcagcg gattcataac 42120
aagtataacg tataacgccc ttgaccctga aaccccggcc caatgttata aattaagctt 42180
tctttatata gattgatttt tagttattat acttattaaa tttattatat ttccttattt 42240
atcaaggact tagcagctat acagcccctt caattcgtat aacgggcttt ttgtgttata 42300
acgttgttga catacaaact ttataaggct atgattccgg cacgcactaa cgaaaggggc 42360
ttgcaatgtc ttacgaaata atcgaatgtt cgccgaaggc gcgaagcaaa attaagtcgt 42420
tcgaagatgc gctaggcgtt gacgccgaat tgacgcacca acgttcgaag tatccttggc 42480
cgctgttgaa aatcggccag tcgttcgccg tgccgttggc cgaaggtaac gaagcatcgt 42540
tgcgaaacgg cgcgtcgcag ttcagcaaga agaccggcaa gaagtttgcg gttattcggc 42600
attctgaata cggctgtttc gaatgcgcgc gcatcgctta gcgccgggcc aatgtgggca 42660
acaaccgaag gggcaaccaa tgtacgaaat cgaaaccggc cataaagtgc cgaagcaacg 42720
tcgcaaggct ggaagcgtgc cgaaggccgt tcaacaggcg ttcaacgact acgccgaagc 42780
gtataaggcc gtttacggcg ttcgcccgct gtcgttcacg tatgaccgcg aaagcggctt 42840
tattcgtgtc gaaagtagcg gcggcgtaag cgtcgcaagg ctgcgcgaaa tgacgaaaca 42900
gcttcgttac cgaaaaggat aaaaaaattc gt 42932
<210> 15
<211> 42932
<212> DNA
<213> Artificial Sequence
<220>
<223> s4 phage变体
<400> 15
cgcacaattg cgccggtatg tacgaactat acgacggccc ttcgttactg tcaagcccct 60
ttttcaagaa tttttcgttc ttcgaacaag tcgcgattgt tggcgtcgaa ttcggtaaag 120
gcgttcggga agcgacgacg aagcttcgcg atgttgaccg actgcgcttc gccgaagttc 180
gaaccaatgg cgcgcagcaa aagcgcatcg taccaaaagc cgtcgccgac ttcttcaagc 240
gcgttaacag cgtcgaaagc ttcgccttcg gctgcggcgc ttagggcttc caacagttcg 300
cccgcttcgg tcgccttgcc gataattgca tgaatgacgt tcacggctgc ggcgtcgtct 360
tcggggcggt cggaaatcca ttcgggaagc ttcgccagcg ttgccgcgtt ggcttccggg 420
gccggaacgc cagcgtcgcg accgtagaac atggttttct cgattgcgtc aagcttgtta 480
agggcttcga cggcttcggc gatggtttcg cggaagtagg ccagcgacac gcggtcgccg 540
taatacttat ccgacgcggt tacgtgcgct tcttcgatat agtcgaaggt ctttgcgggt 600
tcgttcatac gttcaccttt tagaagggcc gggcaccatg cgccagcgtt ccagcactgt 660
aaccgaccgc gcctttcctg tcaacaacaa ttgacagcaa gcaagaagcc gggtaagctg 720
cccacaattg ctagaaggtg gcgttatgga cattgaagaa cttacgaagc gggtaaagaa 780
agcaagcgtc gggcggtcgc tgcgggcgct ggccgcgcaa tgcaacgttt cgcacgaact 840
tataagaaag cttttgcttg ccaattctgc gccgaacata acggcgacga cttataacaa 900
gattgataaa ggattggcga agaatggact ttaacaatat tccgcacgaa atgcggattt 960
acccgcaatg ggtcgtttgg cgatacgaag acacggattc aaagaaaccg acgaaggttc 1020
cgtattcagc aaaaaccggg caccttgcca gcgtaaccga cccgaacact tgggccgggt 1080
tcgacgaatg cgttaacgct atgtcgtccg gttggtatgc cggaatcggt ttcgtattga 1140
ctgaaaacga cccgtattcg tttatcgacc ttgacgacac aaaaggcgac caaacggcgc 1200
ttgaccgcca aataaagatt tttaacgaat tcaacagcta cgccgaacgg tcgccttccg 1260
gttcggggtt gcatatcatc gttaagggcg cgattccgtc gggccgccgt cgttcgttta 1320
tcgaagttta ttcttcgctt cgttatatga caatgaccgg cgacgtttac cgcaacgcac 1380
cgattaacga ttgtaacgaa ctgttaaacg ttctttgggg ccaaatgggg caaggttcgg 1440
tcgccgtcgc gcattatgcc ggacttgccg aagccaagga aaccgacgaa caggtttata 1500
accgcgcagt cgccgcagcc aatggcgaca agttcgccga actgtacgcg ggcaaatggg 1560
aaggcatgta cgcttcgcag tccgaagccg actttgcctt ggtcgatatt atcgcgtttt 1620
atacgcagaa ccgggcgcag atttcgcgaa tgtttcgcgc gtcgggcttg gggcagcgtg 1680
acaaagcaaa acgcgacgat tacgtttcgt acatgctgaa caagtgtttc gaccgcatgt 1740
tgccgcccgt tgacgttgac ggattgcgca acaagctaga cgaagccata gcgaagaaag 1800
aagccgccga cagggccgcc gcgttgtcgc agaacagcga agcgacgccg catccgaaag 1860
ccccggcccc gaaccttagc gaagtttcga aggtatacag cgtgccgccc ggattagtcg 1920
gagaaattgc gcaatacatt tacgcacaag cgccgcgccc ggtgcccgaa atcgcgttgg 1980
ccggtgccct tggcttggtc gccggtattg tgggcagggc gtacaacatt tccggcactg 2040
gccttaatca atacgttctg ttgttggcac cgaccggaac aggtaaagaa gccatcgcaa 2100
gcggcattga taagctaatg gcgcaggtta tccgaaccgt tcccgctgcg tcggacttta 2160
tcggccccgg tgaaattgcg tcggcccaag cgattattaa gtatatgtcg cgcggcccaa 2220
cgtcgttcgt ttcgttggtc ggcgaattcg gcatttacct tcaacaaatg gcaagcgtaa 2280
acgcaccgcc gcaccttacc gggcttcgtc gcttcctgtt ggatgcttac aacaagtcgg 2340
gcgaaggcaa ggtacttcgg ccttcgattt attcagacaa ggacaagaac acgaccgccg 2400
ttctttcgcc gtcctttacc ttgcttggcg aatcgacgcc cgaaaagttt tacgaaggtt 2460
tgcacgaagg tttgatttcc gaaggtctgt tgccgcgttt tacgatgatt gaatatcacg 2520
gcgaacgccc ggcgttgaat ccggggcacc tgtcggccca accttcgttc gaacttatcg 2580
acaggctttc gaccttgtgc gcccatgcgc taatgctgaa cagccagcat aaggcgattc 2640
acgttcaaac cgacgcaacc gcccgcgaac tgttccagca attcgacgcg cattgcgacg 2700
cgaacattaa cacaagcgac cgcgaagttc ggcggcacct ttggaaccgg gcgcacgtaa 2760
aggcgttgaa gctggccgga attatcgccg tcggctgcaa cccttacgac ccgaccatta 2820
ccgccgacgt tgcgtcttgg gcgattaacc ttgtcgtcgc cgacgttcga aacttgcttg 2880
cccggttcga tgctggcgaa attggcatag acaacgacga aacgaaacag cttgcgaagg 2940
ttattgcaac cgttaaagat ttcgtcgttt caccttggcc ggacgttgcg aagtacgcag 3000
gcgaaggaat gtccaacctt cattcgaacc gaattgttcc gtacagctac gttcaacgca 3060
ggcttgccgc tgttgccgtc tttcgtaaag accgcatcgg cgcaagcggg gcaatcaagc 3120
gcgccttgaa gaccctttgc gaacgcggcg atttgcagga agtaagccgg gccacactgg 3180
cgaaggatta cggaacaagc gccgttgcat acatggtcgc gcatccgggc gttttcggcc 3240
tgtaacgcgc gcccgccagt gtgggcagaa cgggccgcct tcgggcggct ttttcgtgcc 3300
tgtaaaaaaa agttcgtcgt acctgttgac gtagcttcga actgtacgta gaatacaaac 3360
catcgaagca acgaaacaaa ccaaggaacc gaagccatga acgtaaccaa ccttaaaacc 3420
gccgtcgaaa tcaaaattga aattgaattg gccgcttggg ccaacggcga aaagaaagtt 3480
aacgtttcca accaattcga cgaaagcgtt ttggaatcgg ccaaagaagc aattagcgtc 3540
gaagcaatcg ccgcaatcgt tgccaagtat ccgggcgcga acttcgaagg cttttcggtt 3600
atgacttggg cgcacgaact ttcggcgttc aaagccccgg aaatcgaccg ggcttggttc 3660
gactaagcaa gcgcccttcg gggcgttttt tttgttcgta cctattgacg aagcttcgaa 3720
gttgccgtat atttaaacca tcgaagcaaa caacgaagga ctaacgaaca tgaacgcact 3780
taacaacatt caacgggtcg taagctggaa cagcaagaag aacgccgacg gcacctttac 3840
cgctaccgcg tacagcttcg accataacgc gccgaccgtc gtacatgcca gctacacgcg 3900
cgccagccgc gcccaagccg tcgcagacgc caagaaggcc gttcgttacc tgaaagccca 3960
acagcgcacc gcgtaaggag tcgccgccat gtttgcccat tacttcgcca gcaagaaaac 4020
gaagttcgtt gtaatcagcg aaacgaaccg cccggaaggc cagcgaatcg ccgtaagcgg 4080
caaagccgaa gcccgcaggg tcgccaagga acgcaacgcg cagccttgga acttctaacc 4140
ccggccacgg cccttggcac gcgctagggg ccgcgcagcc tgcccacatt aagcgaagga 4200
cgaccgacca tgcgtacaac ccttgcagcc gtaaacaaag cccttgccgc cgctggaatc 4260
gacgcggaat tggtcaaggg tcgcgggtac ttttacttcg ttggttccgc cgtagattgc 4320
gccaaagaac aaggcgttta cggcgtgtat cggctttccg aattgaccgt ttcccaatgg 4380
gtcgaagaag cccgccaacg tatcgaaaag gagtaagaac aaatgaccgc atattttatc 4440
gcatttggcg tcgcgcttgg gttggccctt gcgcgcttgg ccgcttccca attcgaagcc 4500
gcttgttacc tgttctgcaa cggcgaattt cgaaaggcat tccgggcaac gatgcacgcc 4560
cttacctgcg ttgcgttgct tgcgttggtt gtttcgcccg ttgcttcgtt cttcgatagc 4620
ttcgaagtta taagcattga acaaccttcg cccaatcctt cgaagggtct tcgcgtataa 4680
tagccgacgg gtcgtataat gggcacgtat aagccttaaa cccttgatac ataaggcttt 4740
cagcggattc ataacaagta taacgtataa cgcccttgac cctgaaaccc cggcccaatg 4800
ttataaatta agctttcttt atatagattg atttttagtt attatactta ttaaatttat 4860
tatatttcct tatttatcaa ggacttagca gctatacagc cccttcaatt cgtataacgg 4920
gctttttgtg ttataacgtt gttgacatac aaactttata aggctatgat tccggcacgc 4980
actaacgaaa ggggcttgca atgtcttacg aaataatcga atgttcgccg aaggcgcgaa 5040
gcaaaattaa gtcgttcgaa gatgcgctag gcgttgacgc cgaattgacg caccaacgtt 5100
cgaagtatcc ttggccgctg ttgaaaatcg gccagtcgtt cgccgtgccg ttggccgaag 5160
gtaacgaagc atcgttgcga aacggcgcgt cgcagttcag caagaagacc ggcaagaagt 5220
ttgcggttat tcggcattct gaatacggct gtttcgaatg cgcgcgcatc gcttagcgcc 5280
gggccaatgt gggcaacaac cgaaggggca accaatgtac gaaatcgaaa ccggccataa 5340
agtgccgaag caacgtcgca aggctggaag cgtgccgaag gccgttcaac aggcgttcaa 5400
cgactacgcc gaagcgtata aggccgttta cggcgttcgc ccgctgtcgt tcacgtatga 5460
ccgcgaaagc ggctttattc gtgtcgaaag tagcggcggc gtaagcgtcg caaggctgcg 5520
cgaaatgacg aaacagcttc gttaccgaaa aggataaaaa aattcgttcg aagggttgac 5580
gtactgtcga agccgtcgta ctatataccc ataccaacga acacaaggaa ccgccgaaat 5640
gcaaaacgtc caaatcaaac gccccgcagc gattaccgaa ggccaagcaa tgaacgcagt 5700
tttcgacgcc ttccgccaag tgtggcgcaa cgtcgaagcc cgcgccgaaa tgcttaaagg 5760
cgtttcgcag tcggtcaagg tcggcggctt cgaagtgtcg gttttcgcat ggcgcgacgg 5820
tcgcattcaa gccaacgttt acgcaggcgg ctttaacccc gtcgcaaccg ccgaagttga 5880
cgaattcgac gcctaatcaa ccaagcgcgc cgggcgcaac ccggcatttt tgaaggagtt 5940
ataacaatgt gcatcgtatg cgatattaaa caggccgtcg aagcttccgg cccgaacaaa 6000
gaagaccgcg ccaaaatcct tggcttggtc gaaaagctgg ccgaagcctt gggcgatgtt 6060
ctggaagtcg cgcaggaagt acacgaagcc aagcccgaag cgttccagcc ggaacacgtc 6120
gaacgcttgc agaacgccgc cgacttgctg aacgaccggg gcgaactgcc cgacgccctg 6180
ttggccctgc tgttggccgc ccttgggcgc ggcgtcaagg tcgaaacaat ccgcgtcgaa 6240
ctgcgcgaag gcgaaacgcc agaacaggcc gtagcgcgtg taatggccga acgcgaaggc 6300
ggggccgcga cgaaacacta accaaagcgc cccggctgtt gtatagtcgg ggcttctttc 6360
tatcatgggg aacgaaagaa catggcggac gaagtagacc aaacagccga ccgaatagaa 6420
aacgaagtaa gctttacggt cggcgaactt tgccgaaagg ccgcagcgat acccgcaggc 6480
cgtccgggcg aatgtgattt ttgcggcgaa cacttcgcgc gcgttgtcga agtgaccgac 6540
ccgcgaacgg gcgaagtcgt cgaaagctgc ggtcgctgtc gtgaccgaag gggcatcgca 6600
tgacgcccgc aacccttgcc aagtctggca ccgaacacgc gcaccaaacg gcgctttttg 6660
cctatgctgc cgtcgcatac ttacacggct tcgacgttgc cgacgaatgg tcaaagaccg 6720
gcaagctacc gaagcgcgac ccggacgcac cgccgaaggt gcccgccctt gaatggttcc 6780
acgccatacc gaacggcggc agtcgcggcg acgacgacaa gtcgcgaaag atacgcggcg 6840
cgcagttgaa ggccgaaggc gtgcggcagg gcgtagccga tacgttcttg ccttggcctg 6900
tttacgaacc gtgcccaagc gacccgcaaa taatgatgat gaaatgggcc ggtctttata 6960
tcgaaatgaa gaagccgacc gaacgtccga agtcggaaac cgcaaagggc ggcgtatcgg 7020
acgaacaacg cagcttcggc gaatacgcaa agcgcgtcgg ctatggttgg gccgtctgct 7080
atgattggga acaagcggcg tcgtaccttc gttcctatat cgaatggggt tcgtaatggg 7140
gctttcgtgc cttgcctgcg aacttacccg cgcccggttg aaagcggcgg cgctggccct 7200
tgtgggctgg ccccttgacc gcatcgccgg gcatttgtcg tcatgctatg gcgaacggta 7260
ttacgtcgaa ggcggcaagc tgtatcgcgc gtcgaagttg ccgccgtatc aaccgcactt 7320
gataaggggc gaagaatgaa aaagaccgac gaagaattga tagacgaagc ccgcagcatt 7380
gcgcgttcga aacgctgttt cgtcgtaacg aagccgggtt attggctgtt ataccgcgaa 7440
tgcacgccgc gtaacgtatg cgtcggcaaa cggtcgtcgg ctgcggcgtt gcttcaactt 7500
gcgaaaaagg cttgacgtag cttcgaagct gtcgtacact tcttcgtaag ttgaacgaac 7560
aaaggagtaa cgaccatgcc cgcatataac gtaacaaccg aattcgaaat gaccgacggt 7620
cgcgttctgt cgtgcgaata cgtcttgtcg gttacgcccg gtcgctattc cggcccttgg 7680
gaagattgtt acccggacga atccgacgca ggcgaaccga cgtattttat cgacggcgaa 7740
gaagtcgatt acaaagaact tccaaaagga ttggacgcaa tcgccgacaa gctttacgaa 7800
gctggcgacg gcgaatacaa ttacaaggaa gaacgcgacg aaccggatta cgaaccggat 7860
tacgattacg attattaacg aaggagtaac gaaaatgtct tggcttattg gttgggccgt 7920
actttccgtc ggcgttgttg tcggcttctt gcttgcggcc ttgctgcggg ccaacgggcg 7980
cgacgtgccg gttccttcgg ctaccgttac cgccctgtcg ccgcaggaac gcgcccgcat 8040
gggctacgcg ctggcaaggg ctggcgactt gctggacagc ggcgaccttg aatacgtgtc 8100
gggcggcttt atggtgcaag acgtaggaca cggcgtcgta attatcaaag gcgatttgtc 8160
gttgcgggtt gccgaagatg aataaggacg aagcgcgcga aatgttcgaa gccgtcaagg 8220
ccaaccgggc gaagattgac ggttgcgccc gccatcgctt cgaaatcggc gacccgccgt 8280
acacgttcgg gcaaaagttc acttgttcga actgcggcgg aacaatggac gccgtacagg 8340
cgttccgata ctgtcaaggc ttcaaggccg caggcggcga cccgaacgaa gttataccgg 8400
gttacgaata acgcttgaca agcggcgaag gttcggcgaa acttagacgt aacgaagaaa 8460
ccccgttaag gagttgccga aatgaccgtt ctaattttgc ttctttgcac cgttgccgct 8520
ttgttcattc atcgcatgtt tgttcgtccg attctggatt ggttcgaaca acgcgaatac 8580
gccgaacgta tgcgcgaagc tgaacagcgc gcggcgttgg aagaagcaag gcgggccaag 8640
tgtcgataat ctacgcaccg cgcgacgccc tgtcgaaatg ggacgcgcga tttattgaac 8700
tggccgaaca cgtcgccgga tggtcgaaag ggccgcgtaa gcgcatcggc gcggctatcg 8760
tgcgcccgaa tcggtcgatt gcgtcgcttg ggtacaacgg cccgccgcgc ggcttcgacg 8820
acgccgtttt tctgaacatg acgcgcgacg aacaacacgc ggtcgtaatt catgccgaag 8880
acaacgcaat tcgacaggct gcgccgggcg aagacttgtc cgcgtgtacg ctgtacgttt 8940
cgccgctgtt tccgtgcgcc atttgtgccg accgcattgt tcgggccggt atccgtcgcg 9000
ttgtcgctta ttgtgggcat atatcgccag actggcgcgc gtctgccgac gaagccgaac 9060
gcatctttaa ccaagcgggc gtcgaatgcc tgtttacaat ggattaacga acatgaaacc 9120
gctagaccat tcgcaaatcg aaggcttgtt gttctatcaa gacccgacaa cgcaaaacta 9180
tatcggcgca ccgaaggccg acccgttcaa ccaagccgga agcttcgtcg cattcgggcc 9240
gcgtgttacc gttgacccgg cgttgtataa cttgcttgcc gcagcgccga caatgtacca 9300
agccctttcg atgcaatacg cttaccttca agcgcaaatc gaagttatcg aacgcttgcc 9360
aaaggcgggc gaacttgata agctgttgtc gtcgctaatc gaagcgcaga acgcttgcct 9420
aatggcccaa cgtatcgcgc aagtcggtta cgaaaatgtc gcaaattcac ttgacaaagg 9480
ttcgaagcgg tcgtaagctt gaaccatcga aacgaacaaa ggtgcccaca atgaaccgta 9540
agcttcttgt tatcgctgga atcgtcgcct tcgtcgcgtt gcttggcatg gtcgggcaaa 9600
tggacttcga agacgaagcc cgcgcgcacc tgttgtattg tgacaacgta gcgaacggcg 9660
tttggccgga ttacgacaaa atttacgatt ctgaatgcac gcccgaacgc atcgcagaaa 9720
tcgaagaaat tttgcgttga ccgcttgaca cgaagaacgg tcggcgtaaa atcaacccgt 9780
cgcaacaacg cgacataacc ttaacgttaa ggagttacac aaaatggcta ccaagaaatc 9840
gaccgcgaag aagaacaccg ccgccccggt tatcggcctt gccgaaattg ttgcagccgg 9900
gccgaatggt ctgtatactt cgcctgccgt acatggcccg cttgtcgaag ccggtatggt 9960
ggaaatcaac ccggcaatga ccaacgaagc gggcgaaatc gcaacccgcg caacccaaaa 10020
aggaatcgac gaaatgaacc agaacaccga aaccggcacc gccgcagctt ccgccccggt 10080
cgctacttcc ttcgccatcg aagacaacgt acccatgccg accggcagcg gtcgcggtcg 10140
cggcggcaac gtctacccgt tcgacgcgct ggaagtcggc caaagcttct tcgttccgaa 10200
cagcgaagaa aagccgaacg ccgcgaagtc gcttgcttcg accgtttcca gcgcaaccgc 10260
ccgtttcgcc gtgccttccg aagacggcgc gaccaagacc aacaagaagg gcgaaaccgt 10320
gcccgtcatg gtcgaaaccc gcaagttcgt tgttcgttcg gtcgaaggcg gcgcgcgcgt 10380
ctggcgcacc aagtaagcga cggcgcgggc tacggcccgc cctacgccta cgcttcaacg 10440
gaaaccccgg ccattgcgcc ggggttttct tttgcttttc ttgtcaattt gtgctaatcc 10500
gtgctaatct tcggccaaat acatttcggg gccggggctt gtgatgaatg aacgaacaac 10560
aattttttaa cgtagccgtc gcaattatcg gcgcgttggg cggttggtgg atgcgcgtta 10620
tgtggcagtc acttaaagaa cttcaagcgc aagattcgaa gttggccgat aaagtcggca 10680
atatcgaagt tcttgtagcc gggcagtatg tcaagcgcga cgatatgaac cgcgacataa 10740
tggcgatttt cgccaaactt gaccgcatcg aagacaagat agacaaaaag gccgacaaat 10800
gaagaagccc gaacttatcg aagattggcg cgaatgctgg cgcttttggt cggttcgact 10860
ggccgcagtc ggcaccgcaa ttactggcct tcttgttgcg tttcccgacg cagcgttggc 10920
cgcttgggca atccttccgc acgaattgaa gtcgtcgatt ccgccgcagt atatgccgct 10980
tatcggcgtt gttgttttcg cgctttccat cgttgcccgc cttgtcaagc aaaagaactt 11040
gaacaagggg ccgaaggatg aataacgaac ttccttgggt agccgaagcc cgaaagtata 11100
tcggccttcg cgaagacact tcgaagactt cgcataaccc gaagcttctt gccatgcttg 11160
accgcatggg cgaattttcc aacgaatccc gcgcttggtg gcatgacgac gaaacgcctt 11220
ggtgcggtct gttcgtcggc tattgcttgg gcgtagccgg gcgctacgtc gtccgcgaat 11280
ggtacagggc gcgggcatgg gaagccccgc agcttaccaa gcttgaccgg cccgcatacg 11340
gcgcgcttgt gaccttcacg cgaagcggcg gcggccacgt cggttttatt gtgggcaagg 11400
atgcgcgcgg taatcttatg gttcttggcg gtaatcagtc gaacgccgta agtatcgcac 11460
cgttcgcagt atcccgcgta accggctatt tctggccgtc gttctggcga aacaagaccg 11520
cagttaaaag cgttccgttc gaagaacgtt attcgctgcc gctgttgaag tcgaacggcg 11580
aactttcgac gaatgaagct taaaggggca tcgcatgttt tccgtaattt gggccaagtt 11640
tggcaaatgg cttgccgcaa tcggcgcggg tcttgtcatg ctggcaagta ttttttatgc 11700
cggtcgcaag tccggcgcaa ccaaggccga agccaacgca gcaaacgaac gggcaaacga 11760
ccgcgaagca atcgcagttc gccaagttaa cgaagcccgc gaagcttccg aacaacaggt 11820
aaaggcggta caaaatgcga aagacgttgc ttccgataat tctacttttg acgacgacga 11880
agttactaag cggctgcgcg acgaatggtc gcgcgactga accgcaaagc atcgccgacg 11940
cagtgcaaac gaagcccgtt gtaatcgaca cggcttgcaa atgggttgcg cctatttgga 12000
tttcgaaggc cgacggcttg accgctggca cggcccggca aattctgaac cataacgaag 12060
ccgtcgaacg caattgcggg ccgcagtcgc cgcctaaatc tgcggacaag ccttaacaga 12120
acgcggggct tgcggtatag tgaagccaac gacgtataaa ctaggccgac actatgtccg 12180
acgaactgaa agaaagcgaa gaaaaggccg catacgcggc ccttcttttg aaagaacgcg 12240
acccgttcaa ggctgcgctt tcgctgtttc cgaacaacac gaatcgcgcg ctatgggttg 12300
cgaaccattg gccgaacgac gccgaagtta aggccgaaca aaagcgcctt accgacgaag 12360
gcggcgatat ggcatttctt ccgggcaaag gcgaccttgc ccgcgacatt tggcagcgta 12420
tgcaaggtac gacgcttccg aacggcgtaa ccattccgcc gacgcccgaa gaatacgcga 12480
agcttgccaa gctttacgcc gacgttcgcg ggttcatcga aaaaccgcaa acgaacgtta 12540
acgtaacgac gaacgtaaac cgcgtcgtcg aaatgcccgt attcgctaac gaaagcgaat 12600
gggaagcgga agccgcacgg caacagcgcg aattgttaga aaatgcacgc actcgccatt 12660
gatacgaacg gggcgaaacc tgttccgtat gaagtcgtat ttaagcccct tccggggtcg 12720
cagactatcg ccctttgttc gatggcagcg catacgctgt acgaaggcgc acgcggcccc 12780
ggtaagacgc ttacgcagct tatgcgcttt tatcgtaacg tcggcaaagg ctacggcaag 12840
ttctggcgcg gcgttatctt cgatttggaa tttgaccatt tgggcggcct tgttgccgaa 12900
tcgaaaaaat ggttcggcga taacggaaag ctaaaagacg gcggcaagtt ttacgaatca 12960
acgtcggctt ataaatgggt ttggccgaca ggcgaagaac tgttgtttcg gcacgttaag 13020
aagctgtcgg attacgaagg cttccacggt cacgaatacc cgtttatcgg ttggaacgaa 13080
cttacgaagc atccaagcgg cgacctttac gacaaattta tgtcggtcaa ccgctgtacg 13140
ttcgacccga taaaagacac gccgaaagac ccgaaaaccg ggcgctattt gacgccgaac 13200
ggcgaaccgc tgccgcctgt aaagtgcgaa gtattcagca caacgaaccc aagcggcccc 13260
ggtcacaatt gggtaaaacg tcgctttatt acgattgccc cgcgcggcac cgtagttcgt 13320
cgcgaaattc agatttacaa cccggcgacc gaaaaagaag aaacgcacgt aattagccaa 13380
atcgctatct tcggttcgta taaggaaaac ccgtaccttc cggcttcgta tatcgccgaa 13440
ctggaaagta tcaaagaacc gaacttgcgg aaggcttggc tttacggcga ttgggacgtt 13500
accgcaggcg gcgcaatcga cgacctttgg caatcgcata tacacgttgt accgcgcttc 13560
gtcattccgc caagctggcg aatcgacaga acatacgacg acgggtcttc gcatccgttt 13620
agtgtgggct ggtgggcgga agcggacggc accgaagcga ccatagttct ttcggatggc 13680
accgaattta cgttttgccc gcaaccgggt tcgcttgttc agttgttcga atggtacgga 13740
tgcgcgaaag acgaaaaagg cgaatacata ccgaataaag gtttgaagct ttcggcgtcg 13800
aatatcgcac aaggtataat cgaccgcgaa atttcgctaa tggcgaacgg ttggatttat 13860
tcgcagcctt ggcccggccc tgccgacaac cgcattcgcc aagtaatcga cagcgaacta 13920
gacacgaccg aaaagcttat gtcgaagaag ggcgttcgtt ggacggaatc ggacaaatcg 13980
ccgggttcgc gcgtaatcgg gttgcagctt ttccgcgacc ggctagaagc gtctgttaat 14040
cgcgaagggc cgggtatata ctttatgtcg aattgcgttg caagtattga tttgctgccc 14100
acactgccgc gcgacgaaaa gaagattgac gacgtagaca cgaccgccga agaccattgt 14160
tacgatatgg tgcgttatcg cgtattgaaa ggcgcgaaca aagcggcagc gaaattcaaa 14220
cttgttatgc caacttaaag gaacccaaat catgccgaac gtttcattta ttcgccccga 14280
acttggtaag ctgctgccgc tgtattacct gattcgcgac gcaatcgcgg gcgaaccaac 14340
ggtaaaaggc gcgcggacga cttatcttcc gatgccgaac gccgaagacc aatcgaaaga 14400
aaataaggcg cgatacgaag cgtacttgaa gcgggccgtt ttctataacg tagcccgtcg 14460
aaccttgttc ggcttggtcg gtcaagtgtt catgcgcgac ccggttgtaa aggttcccgc 14520
gctgttgaac ccgcttgtcg caaacgcgac gggttcgggt atcaacctta cgcagcttgc 14580
gaaaaaggcc gtatcgctga accttgcgta ttcccgcgcc gggctgttgg tcgattaccc 14640
gacgaccgaa gccgaaggcg gcgcgtcgat tgccgacctt gaagccgggc gcattcgtcc 14700
gaccctgtac gtatattcgc cgaccgaaat tatcaattgg cgaacgaccg accggggcgc 14760
ggaagaagtt ttatcgttgg tcgtactgtt cgaaacttgg tgcgctgccg atgatggttt 14820
tgaaatgaaa acgtcggggc aattccgcgt tttgcgtttg gacgaagaag gttattacgt 14880
ccacgaaatt tggcgcgaac cgcagccgac caaagccgac ggtacgaaga ttccgaaagg 14940
caattaccaa caatacacgg tttacaagcc gacggacgca aacggcaatc gtcttaccga 15000
aattccgttt atgttcatcg gttcggaaaa caacgattcg aacccggaca acccgaactt 15060
ttacgacctt gcgtcgctga atatggcgca ttatcgcaat tcggcggatt acgaagaaag 15120
ttgttatatc gtcggacagc cgacgccggt tcttatcgga cttaccgaag aatgggttac 15180
gaacgttctt aaaggttccg ttaacttcgg ttcgcgcggc ggtattccgc ttcctgccgg 15240
ggccgacgcg aagttgcttc aagcatccga aaacaccatg cttaaagaag caatggatac 15300
caaagaacgc caaatggtcg cacttggcgc aaagcttgtc gaacaaaagg aagtgcaacg 15360
cacggcaacc gaagccgaat tggaagccgc ttcggaaggt tcgacgcttt ccagtgcaac 15420
caagaacgtt tcggcggcct ttgaatgggc gttgaaatgg gcggcccgtt gggtcggtca 15480
agccgacagc ggcgttaagt tcgaactgaa tacagatttc gacattgccc gcatgacgcc 15540
ggaagaacgc cgttcgcttg ttgaagaatg gcagaaaggc gcaattacct tcgaagaaat 15600
gcgaaccggc ttgcgtaaag ctggcgtcgc aaccgaagac gacgccaagg ctaaagaaaa 15660
aatcgccaag gataccgccg aagcaatggc ccttgctgcc ccggctaacg tgccgggcga 15720
tggcagcggc ggcgataatg tgggcaacaa cggataaggg gcgcaatcat ggcactatcg 15780
gacaataagc gactgtacga tattgcgacg cgccttgctg tttacgtcga agacgtgaaa 15840
gtttggcagt cccgacaatt cgggttcgta cttcgcgaag taaacttgga attaacgcgc 15900
cttcttggtc gtgttcgtta taagaccctt gacggtcttt cgaaggcgca gttaaacaag 15960
cttgtttcgg aattacggga atcgcaatcg aagatttaca gcgcatacac gcaaacgctg 16020
cttgaacagt taaaggaatt catgcgggcc gacttggaag taaaccggcg cgcttgggtt 16080
acgggttata tcgaacttga cggcgaagaa agcgacgaca ttatttcgga cgaagaagcg 16140
atacagtttc ttttagaagt accaaacgcc gattcgaacc ctttgttcgg tttggccgct 16200
gttacaggaa gcgacgaacg catttggtcg caagttacga atacgccaat tccggcaaat 16260
ggtttgtact tgctgccgtt tatcaagacg tttacgaatt cggcacaagc tggcgtcgaa 16320
agcatgattc gcaaagcttg ggcgaaccgt tggacggttg aagaaacctt aacggcgctt 16380
gtcggcgatg gaaccgcagc gcagggcacg ccgtcgcagt tgcaccgggt caacgcgcaa 16440
gccgcgtcgg taatccatac ggcaaccgcg cacgttgcgg cggtcgttgc ggctggcgtt 16500
atgtctgcgg ttttcggtcg ttacgtttgg tattcggtaa tcgacggaag aacgacggac 16560
atttgcataa gtcgaaatcg ccgaatctat cgcttcggcg aagggccgtt gccgcccgcg 16620
catatccgtt gccggtcgca cgttgccccg gcaaataccg ctagtgactt ggccgaagaa 16680
acgttttata cttgggttgc ccgtcaacct gcccaagtgc aagacgacat attaggcgac 16740
gaaggcggcg aagctttgcg cgatggtcgc ttaaaggcga aggatattcc gaagtatgat 16800
gcggacaggc cgctaactta cgaagaattc cgacgcaaga ttaaagaaat tctttcccgc 16860
tgattcggtg aatcggcata accgcaagga gtccttgaaa tggcactgaa aaagaaactt 16920
accaaagaag aacacgcgaa gctttcggat gcactgaaag ccgaatatat cgaagacggc 16980
gacggcttcc gccttgacgt tgacggcgac gaagatacgg gcgctttgaa gcgcgccaaa 17040
gaccgcgaag cgcagttgcg caaagacgcc gaaaaggaag cgaaggaact tcgcgaacgg 17100
ctggaatcca tcgaaggcga cgacgcgcgc aagaaaggcg acattgcgac gcttgaaaag 17160
tcttggcaat cgaagcttga aaaacagcgc gaagaatacg aagccaaggt ttccaagctt 17220
accgcgcata cgacgaaaac gcttgtcgat aacgtcgcgt cgcagcttgc ccacaagatt 17280
tcgaacgccc cggcaatcat catgccgcat atcaagtcgc ggcttatcgc agacttcgaa 17340
ggcgacacgc cggttacgcg cgttctggac aaggacggca agccgtcggc ccttactatc 17400
gacgaactgg caaacgaatt cgttgcaaac aaggattttt ctgctattat cacagctagt 17460
aaggcgtccg gcggtgccgg taagccttcg cagaacggcg gcggtgcccc gaaatttaac 17520
ggtcaatccg acaaacccgc cgacctttcg aagatgaatc ccgcagaact tgcggcgcat 17580
cttaaagaag cgaaggcaaa cgaaactaag gacgcttaat catggcactt tccgaccttg 17640
ctgtttactc cgaatacgct tattcggctt tctccgaaac cctgcgccaa caggttgatt 17700
tgttcaacgc tgcgacgggc ggggctatca tgctgcaatc cgcagcgcac caaggcgact 17760
tttccgacgt tgcctttttc gccaaggtaa cgggcggcct tgttcgccgt cgtaacgcct 17820
acggttccgg caccgtcgcc gaaaaggtct tgaaacacct tgtcgatacg tcggttaagg 17880
tcgcagccgg tacgccgcct gttcgccttg accccggcca attccgttgg attcagcaga 17940
acccggaagt cgcaggcgcg gcaatggggc agcaactcgc cgtcgatacg atggcggata 18000
tgctgaacgt tggtcttggt tcggtctatt cggcactttc gcaggttgcg gcggttgttt 18060
acgacgcgac cggcaatact gcgcccgacg acggcccgac ttggaacaac cttaacaacg 18120
gccaagccaa gttcggcgac caatccgcgc agattgcggc atggattatg cacagtacgc 18180
ccatgcacaa gctgtacggt aacaacctga acaactccga acgcctgttt acttacggca 18240
ccgtgaacgt tgttcgcgac ccgttcggca agcttcttgt tatgaccgat tcgccaaacc 18300
tgttcgaagc cggtacgccg aacgtttatc gcatccttgg ccttgtgccg ggcgcggtta 18360
tgattggtca aaacaacgac ttcgacgcga acgaagaaac caagaacggc gacgaaaaca 18420
ttattcggac ttaccaagcc gaatggtcgt acaacgtcgg cgttaagggc ttcgcttggg 18480
acaaggcgaa cggcggcaag tcgccgaccg acgcggcgtt gttcacttcg accaattggg 18540
acaagtacgc aacttccgaa aaggatttgg cgggcgttgt cgtcgaagtt cactaacgga 18600
cggcagacgg acggggcttc ggccccgttc ttctagtcct tcacaaattc aaaggagttt 18660
cgaagatgaa accggcaaaa atcctgtatt tcgtcgatgg caacgcaccg accccggaag 18720
acttcgcagc cgccgccgaa ctgaacgcgc aagttatgtt caggaacgcc cgcgctgttc 18780
cgtccgaacc gcattcgctg gaaatctgcg acggcgtagc gggcaaggtt ccgaagctgt 18840
acgccgacgc ttacccggaa gcgggcgaag caatcaagaa gaaggccgcc gaactgaaag 18900
cccttgcttc gaaggtcggc gacgccccgg caccgaaggc cagcggcaag gccgccgaca 18960
agcccgcagc cgctacgcag ggccagaaag ccccggcagc ggccccgcag ggcggcagcg 19020
ccccggcttg gaaccctaac ccggcgcagt aaccaacgga cggcctgtcg cttattgtgg 19080
gcaggccgtt tcaacaacga actaaggagt cttcgcaatg gcaacgaaaa aggttgttta 19140
ttttacggca ggcattaccg caaccgctgg cgaacttgcc gcaattgcga agcttaacgc 19200
agccgccgaa ccgcaatatg aagttttggt agccaatggc gcggcaaatg ccgaatacgg 19260
cgaaaccgac cgaatcattc cgtcggatta tgtcgccggt actgttcctt cggaatattc 19320
ggcggttgac gtaatcgacc ccgacgcaat cccgaatcag gcgcttacgc caacgcaagc 19380
aatcgttaac gacgccgaag ccttgaccgt tccggttact ggcacttaca cgacgaccgc 19440
aaccgttagc gttgcgaacg gcgttgtaac cggcatcgtt ctttcgtaag gacgttgacg 19500
catggcaatt acaatcgttg ttgaagacgg aagcggcgta acaaacgcga acagttacgt 19560
aagcgtcgca gacgcgcgca tttacgcttc gaatcgcggc gtcgaacttc cgttggacga 19620
cgacgaattg gcggccatgc tgattcgttc gacggattac ctagaagcgc aggcttgccg 19680
gttccaaggc aagccaactt cgacgacgca ggctttgcaa tggccgcgaa ctggcgtctt 19740
tcttaacgaa gacgaagtac cgtcgaacgt tattccgaag tcgcttattg ccgcgcaagt 19800
tcagcttgca atggcgatta acgcaggctt cgaccttcaa ccgaacgttt cgccgcaaga 19860
ctacgttacg cgcgaaaagg tcgggccgat tgaaacggaa tacgccgacc cgctgtcggt 19920
tggcatcatg cccacattta ccgcagcgaa cgcacttctt gcgccgctgt ttggcgaatg 19980
cgcttcgaac aagtttgcgc ttcgtacaat aagggtttga cgaatggcac gtttcgacgc 20040
agcaattaaa acggcgcaac gccttatcgc aaagaacggc gaaaaggtga aatggcgcgt 20100
tatcgaagac gcgacgccga cagacccgaa caagccttgg gaacccggcc cggcattgcc 20160
ggacgacaaa gacgttacca tttgcttttt gccggttgac cggcagacgc aagaaacttt 20220
caacttcatt aaaggcaccg aagttccgaa aggttcggta atggggctta tgggcaatgt 20280
tccgtttgaa ccgaatttga aagacgttgt aattcgtaac ggggtcgaac ttcgcttggc 20340
ttatatcgac gtactttcgc cgaacgggca aaaggtactt tatacgatgg tatttcaagc 20400
atgattgaat tcgaccaagt taacgacgaa gtaaacgcgc tttttcttgc aacttggaac 20460
gcaggaagcg cggcaattgc gggttatgtt cccgaaattc gttggcaggg cgtgcaatat 20520
cgcgacttgc cggacggttc gaagttttgg gttcggctgt cgaagcaaac cgtttttgaa 20580
gaacaagcga ccctttcaac ctgcgaagga gtaccggggc aaagaaaata tacggcgtcg 20640
ggtcttgtct tcgtacaaat cttttgcccg aaatcgaata cgcaagcgtt cgaacttggg 20700
cagaaattgg cgaaactttc ccgtaatgct tttcgcggga aatcgacgcc gggtaaggtt 20760
tggtttcgca atacgcgaat taacgaactt ccgcccgaag aactttacga acggtttaac 20820
gtcgttaccg aatttgaata cgacgaaata ggttaaggag ttcttaacat ggtatgcgaa 20880
attgccaaaa tcgacagcaa cattaccgga cttgccttcg ccgaagaaga atgcttgaag 20940
caacttccga cgacgcccgt ttggtacgga ctggaaccca acagctattc ggacttcggc 21000
ggcgaacttt cgaccgttgc ccgcgcgcct atcgacccgt cgcgccagaa caagaaaggc 21060
acgattaccg accttgacgc atcgggcggc ttcaacgccg actttacgaa gaccaacctt 21120
acgcgaattc tgcaaggctt cttcttcgca gacgcgcgcg aacttccttc gacgcagccg 21180
cttaacggcg catcggtcgc acttaccggc gttaccgcag ttgacagcac ttacgccgca 21240
gcttccgggc ttggcgtgtt cggtgccgat atgctggtat atgcgaccgg ctttaccaac 21300
gcggcgaaca acggccttaa aaccgtcgtt tcggctaccg ctgcgggcgt cgttgttgct 21360
gaaaccttga ttgacgaaac cccgcccgca ggcgcgaagc tggaatgcgt cggtcgccag 21420
cttgccgccg cagacgcgaa cattgccgta actggcaacg tcgtttcgct tatcgttacc 21480
gctggcgact ttacgaccat gcccgaactg ttccccggtc gttgggtctt cgtcggcggc 21540
gatgcgacgg caaaccgctt cgctaataat gtgggctatg cccgcattaa gtcggttgca 21600
gcgaaggcgc ttgttttcga cgacgtgact tggcaggcgg ccaacgaaac cggaaccggc 21660
aagtcgattc gtcttttcgt cggaactgtt atcaagaacg aaaagacccc ggcacttatc 21720
aagcgtcgtt cgtatcaaat cgaacgcacc ttgggcgaag gtttgaacgg tacgcagtgc 21780
gaatatctgg aaggcgcagt accgaacgaa tttacgttga acgttccgca ggccgacaag 21840
ctgaacgccg acctttcgtt tgttgcgtgc gacaacactt accgcagcgg cgaccccggc 21900
gacgaacaga aaaccggaac ccgcgtgcct gcgcccggcg aagacgctta caacacttct 21960
tcggacgttt accgaatcaa gatggccgtt cacgacgccg cgtcgtcgaa ccccgccgcc 22020
ctgttcggct acgtttccga agcgaacgtt tcgattaaca acaacgttac gccgaacaag 22080
gcggtcggcg tgttgggcgc gttcgatact tcggcgggta acttcgaagt cggcggttcg 22140
attaccgctt actttacgac cgtcgcagcg gttaaggctg ttcgcgcgaa cgccgacgtt 22200
ggtttgtcgg taatcagtgc ggccaagaac gccggtttcg tgttcgatat tccgttgctt 22260
ggtttgggcg gcggtcgtct gaacgtcgaa aaagacgcgc cgattaccgt tccgctggaa 22320
cccgcaggcg cagaaaacgc gaacggctat acgatgctgt acgaagtgtt ttcttatctg 22380
ccgaatctgg caatgccgga ctaattgcgg taaactcaaa gggccgggta ataccggccc 22440
tttcttcatt caatcggagt aaatcaaatg tctggactgt ttaagcaatt caaaacgaat 22500
tcgaccaagg aagtcgaagg cgtcgaaatc gaatttcccg aagcgcagaa cgacgacggc 22560
agcgttccga ctttcgttat ttcccgcatg ggcaaatcga acaaggcgta ttcaaaggcg 22620
cttgacgcgg cgacccgtcc ttatcgccgt caagtcgaac ttggcacgct gaaaaacgaa 22680
gtcgccgaat cgctgtttat gggcgtgttc gtcgataccg tgttgcgcgg ctggaaaaac 22740
gttcagggcg aagacggcaa ggaaattgcg tattcgaaag acgccgcaat ttcgcttctt 22800
tccgaactgc cggacgttta cgaacgtttg caggaagaag ccaagttggc ttcgaacttc 22860
cgcgataacg ctttggaagc cgaagcaaaa aactaaccga agttttggcg cacctgttgg 22920
aacttggccc acacgaacag gcgatagcaa aacaagcgat gcgcgcgggg cagccgttac 22980
ccgaacgcat cgcgaacgcg ccagaacttg aagtaggctt gcagttgtat ttgcaagcct 23040
tcttcgacct agatagcgaa cggtcgcacg gcaacggttt aacgccgata ccttggacaa 23100
gtatggcggc ttacgcaagg gctttcgagt ttgacgaaga acaaaccgaa gacttgtttt 23160
actttatgcg aaagcttgat tcggaacact tgaagaaact agcggataaa cagaaagcgg 23220
cggcatcgaa tggcaaaaag cctgctagac ctagccgata ggctggaaaa gaaagcgaag 23280
gcaatagacg aagcggcgtc acaaaacgcc gtcgataccg ctttggctat tgtgggcgac 23340
ttggcgtaca aaacgcccgt agatacttcg caagctttgt cgaactggat tgtaacgcta 23400
gaaagtccgt cgggtcaaca aatcaagccg cattttccgg gttcgcaggg ttcaacgcaa 23460
cgcgcttcgg cggctgaaac gttgaattct gcgaagcttg ttttacgaaa caagaagccc 23520
ggccaagcga tatttattac gaacaacctt ccgtatatcc gcaggctgaa cgacggttat 23580
tcggcacaag ccccggcggg gttcgtcgaa cgcgccgttt tgattggtcg caaaatgcgt 23640
aagaagttca agattaagga ttaaaagaaa tggccgacga aaacatagaa attaaggttc 23700
aagacaaagt ttcgccgtcc atttcgacga agcttcgcac aattgcaagc gaagcccgca 23760
acgccgacgc cgcagttaag aaccttcaaa cgcagctttc ggcaattaac gtcggcggct 23820
tgtcgcagct tatcaacgca tcggccagcg caacgcgcca gcttcaacaa ggcgcgcttg 23880
ccgcgcaacg gctggcaacc gaacagcagc gcaccgcaac agccgcagcg caggccgccg 23940
cagcccaaca gcgcgtcgcg acggctgcga cccaaggggc aaccgcgcaa gccaaccttg 24000
ccagcgccac gcagcgcacg cagacggccc aacagcagac ggcgacagcc gcgcagcgcc 24060
tagcgacaga acagcagcgg acagccgttc agacggccaa cgcggcggca gcgaacgacc 24120
gggccgccct tgccgccctg cggctgcaac aggcgcaaga ccgggccgcc aattcgaccc 24180
gcaatgcgac ttcggccctt ggcggataca ttcgaaccgc tgccggaatt cttggcgtaa 24240
cgatttcggc gaacgcaatt cttgcaagcg ccgacgcata cgttacgctt caaaacaagt 24300
tgcagaacgt aacgaagtcg caagaacaag ttaacacgct tacgaacgaa cttttcgaac 24360
tgtcgaaccg cacgcgcgcg ggtattgaag aaacggcgac agcgtttaca cgcttcgacc 24420
gtgccttggc gtttatgggt aagtcgcaag aagattcgtt gcgaatgacc gaaaccatta 24480
acaaggcttt gattgtttcc ggtgcaacag cgcaagaagc gtcgtcggct ttgttgcagc 24540
tttcgcaggg cttcaacgcc ggtaagctgc aaggcgacga attccgcgcc gtttcggaaa 24600
atatgccaat cgttttggac gcagtagcga aggcgcttaa cgttccgatt aaccgcgtta 24660
aagaactttc gaccgaaggt aagattactt ccgaagttct gtttgaagct ttcaagctta 24720
tacaagacca agtagacgca acgtttaaca agacgacgcc cacaatcggc caaagcttga 24780
cggttcttcg caacaatgca attcagttct tcggcgaatt gaacaaagcg accggcttta 24840
ctgccgggct ttcgcgcgcg attatttggc ttggcgaaaa cttgaagacg gttgccgtaa 24900
ttgtcgccgg gcttggcgtc gcattgcttg tcgccttcgg tgccccgttg gttggtgcgc 24960
tggccggtgc gacggctgcg gtaaaagcct ttacgctggc gcttgcttcg aacccgattg 25020
gccttatcgt cgtcgcgctt tcgaccgcga ttgcgtatct aacgcttttc cgcgacgaaa 25080
ttaacttggg aatcgacgac gttacgacgt tgggcgattt cttccgcgcg acctttgaag 25140
gcatcggcca agcaatcgac gacgttaaaa ccatcgcgtc gcaattgtgg gcagaactta 25200
gcgacttcgc aagcgcagcc cttggcgaaa ttacttcgaa cgttgacgat tcgacttctt 25260
cttggtcgga cagttacgcc gaatttttcc aaaccaaccg tacaggttgg gccggtgcgt 25320
tggaaaacgc cgcaaaagtt cttgacgcaa tcgccgggct tttgaccggg gccgcaacgt 25380
tcgcaggccg tgcaatggcc gaagttgtta tttcggttca aaacggaatc gcaaacgctt 25440
acaacgttgt cgcgggttgg attgaaaacg taacgaataa agccattgaa gccgcgaaca 25500
ggcttcgcgc gatggttggt aagtcggctt acgaacttgt ccaattcgaa cgcatgggca 25560
gcgcaggcca aaccgaattc gaatcttggg gcaagctttg ggcgcaatcg ctggaagacg 25620
gatttaacag ccaaggcggc gcaatgcaaa acctgttgaa cggccttttc gaccgttcgc 25680
agcaaatcgg cgcgcagcgc agggccgcag gaagcgcgca gcttcgcggg gccggtacgt 25740
cgcagcttgc cggggctacc gacgcaaacg cggcgaaggc tgcggaacgt cgcgcgctgg 25800
cgttggaaaa aatcaatacg cagcttgata acgaattggc gcgcatgttc cagcttcaac 25860
cgcagcgcga agcgcaagcg aagttcgacc aaatcgaaga aagcttgatt cagaaaaaga 25920
ttaagcttac gtcggaagaa gccgaagcaa tcaaggcgaa gattaaggcc gttcaagacg 25980
cgaccgaagt tcaacgccag ttcgacgcaa tttacgccga agctgttaac cctttgaagg 26040
agtacaacgc ggcgcaggaa gcggcgaaca agcttcttca aatgggcgcg attacgcagg 26100
aacagcacgc ccgcgcagta acgaaggcgt cggaagctta cgcgaattcg caagacccgt 26160
tgcggcagta taaccgcgac cttgaacagc aattgcagct tttgcaaatg ctgccgaagc 26220
aacgcgaaat cgaacagcag gttatgcaag ttcagaacga tttgcttgca aagggcatcg 26280
ttctaaacga aaccgaattg gcgcagcttc gcgaaaagct gttgcttatt cagcaagtaa 26340
acgccgtttc gcagcaagaa gcgtcgttgt tggacgccag cgtaaacaag cgccaacagt 26400
ttatcgacca acttaaagca attcagaact tgcgcaacaa cagcggaagc ggtttcaacg 26460
caggcgacca agccgaagca actaacagca tgttgcaagg catgggcatt gatacgacga 26520
actttcaaac gcagcttaac gcgcaattgg cgcaatatca aacttacgtc gaacagctta 26580
aaatgctgaa cgaacagcgt ttgattagcg accaagaata cgccgccgca agtatgcaac 26640
ttgaattgca gcggcaaaac ctgtacttga attcggcaag tagtttcttc ggaaacttgg 26700
ccgcgcttca acagtcggga aataagaaaa tggcggcggt cgggaaggcc gcagcaatcg 26760
cgcaagcgat gattaatacg tatcaatcgg caacgtcggc ttatgctgcg atggcgtcga 26820
ttccttacgt cggcccggcg ttgggcgcgg ctgcggctgc ggctgcaatt gccgcaggct 26880
tggcgaacgt tcagcaaatc cggtcgcaga ataccgggtt taagtcgggc ggttttaccg 26940
ggtcaatggg cgtaaacgaa gtcgcgggcg tcgtacacgg ccaagaattc gttatgaacg 27000
cagcggcgac caaccgtatt ggcgttgcag accttcaagc cctgcaaacg ggcgcggcca 27060
gtgttcagcg caacgacgaa caggcaatgg cggctagtgt gggcaggggc ggcaattccg 27120
aacccgcgcc cgcgccggtt gtcaatgtac cttttagcgc cgttgtcgta caatcgaaag 27180
aagcggcctt ggccgggctt aaatctgcgg aaggtcgggc tttcattctg gaaactatcg 27240
aacaaaacgg cggcaccgtc gcaaaaatcg tcggagttaa ataaatgggt tacgcaattg 27300
gaacagttac aaaaggcggc ggcgacgatt gccattatca agttttggca attatcaaaa 27360
cgctggccga agcgaacgga tggacgacgc ttcgttacgt atcaaccgga cttgaccgcg 27420
aattgatttt gaaaggcgtt ggtttgtccg gtcttgaaga aatctttgta gggtttaagt 27480
gttatcaaag cgtcggcggc gattattaca atattaattg cggaactttc gtcggttacg 27540
taagcggaaa cactttcgaa acgcaacccg gcgcgcagtt tagcgcaacg cctgcccaca 27600
ataacgcgat tacgtatttc ataacggcca acgcccaacg aatcgtcgga tgcttcaaag 27660
tcggtacgcc ggtttacgaa catttttacg tcggcaagtt ctttccgtat gcgcggcccg 27720
gtgaatatcc gtcgccgctt gtttgcggtg cgatgctttc caacgcgcag gcaatgcgat 27780
tcgacaacaa cgcgcagtcg ttcccgtatc cgggctatta ttacgacacg acgaacaacc 27840
gcctttatat tcgcgaccaa gcgggcaact ggattcgccc ttggtgttgg ccatttacca 27900
atggaaacgg cgcaaacacc gcgcttgcag ggccgcaagg aacttcgacg cttgttcctg 27960
ccgacgtgta ttaccaactt gaaccaatta ttttggcgca gcaaagcaac aataacaacc 28020
cttcgaacgt atggggcgaa ttcgacggcg tttatttctg ttccggcttt aacaacggcg 28080
tcgaaaacgt agttcaggaa ggcggaagtt ctgtcgttga ccaaaccggg cttaccgtac 28140
ttcaagccgt agacgctatt aaggcggtcg gcgggcgggc gtttgtaatg ttgcaaaacg 28200
tctatcgtac ttcttggcgc gattttatcg ctttggaaat gaaataatgg catacattac 28260
aggaaccgcc gcaaactttg cggatttgaa aacagccatt gaaaacgcct gtacttctaa 28320
cggttgggcg cttgccgacg gcatattgtc gaagaacggt tgtttctttc aactaatcgc 28380
cgatagtttg ggcggttatc cgcaacttcg cttgcatggc ggaacaggca aaagcggttc 28440
gacgcttaca ggccagccgc ctagcaatta cggggcaaag gtcgcaagcc ctgtcggaaa 28500
tcaaatcgta ttcccgatta attacgaaat acatttgttt accgaccccg acgaagttta 28560
ttgtatcatt aattacaatt cggactttta ccaacaactt tcgttcggca agtctgatat 28620
tccgggcatt ggtggaactg gcgcatggtt taccggggca tatcggcacg actacggatt 28680
gaccgacgca acttacgccc atagggtttt tacggcgatt tcttggcccg aaataactgg 28740
cgtcgcaact tatgccggaa tgtgcgtcgg tttgtttttt gaaggcaacg cttcgcaaac 28800
tggcggaagt tatttcgcaa gcttcgtaca taccgggctt gattcgacgc cttggcgaag 28860
cggccacggc ggcgacacgc tgttaagcgg ctatcgcggg gccggtcttt ccgtcggttc 28920
gcttttaatg tcactgccga atatttcgaa ccaagcaacg attttgcttc ctattaagcc 28980
ggttgcgcgt cgttcaagcg gcggcgcaac tattatcgca aacccgaaaa acgcgcgtta 29040
tttgcgaatc gacaacgttg taccgggcga aattattaca ttcggtgccg accaatggaa 29100
ggtttacccg ttttaccgca aagacgcaac cgttcgaaac ggcgtcggtt ggtcaagcgg 29160
cgcgacgcat agcggaacgt tcggatacgc cattaaatac actggaactt aaaaagaatg 29220
gccggaagaa ttggaacatt tttagcggcg gaaagtttta tttacggcga ttataacccg 29280
aacctttccg ccgaacttaa cgaacttacc gccgcagaat acgcgccgac gatttacgac 29340
ccgcgcgata tgttcttgcg cggcggatat tcggcgtaca aaatcgcgcc ttcgaatcgc 29400
tggcctattg tgggcaaaga acaaggcaac ttcgggcgcg actattatta tcgcgttcac 29460
gtttcgccat tggtcttgga cttgcaaacg gtcgcgtcgt cgcaaacgcg cgaattcaac 29520
gtttggaacg catggcccga ccgtgcggct aatcttgacg acattttggt aagtaatccg 29580
gtcggtattg aaattacagg ccaagcgacg ccgtatgcaa tgccgccgct tcaagaactt 29640
acttacgaaa taaccgtagg cgtttcgggg ccgccgaata ttaacgttga agttcaattt 29700
gacttttcca acgttcccga cccgctgccg attctgatta caggaacgcg cgcggttaag 29760
ttcgacattg tgcccgaagt tccattgaac gaaacttggg aatggttaag cgacaacatt 29820
gtagcggttg acggaacaga acagcgcata gcacttcgcg gcgaaatgcc gcgtattgaa 29880
gaacgcttga aagtaatttt tgattctgtc gaagccgtgc gccgttttta cggcgacgtt 29940
atgtcggcag ttggtcggct ttggattccc gaatttcagt acgcgacccg aattactgcc 30000
gcaagcaata gcggcgcgct tcaaatttat tttgaccgaa cgaagaccga cgttcgcgat 30060
accgaatacg tcttgattca aacgccgctt gttgcggcac ttgtcgaaat cgacgtattg 30120
acagccgacg gggctacgct tggttcgccg ctgttgttcg acattccggc agggtcgtta 30180
atcatgccgg gttcgcccgc gcttattgac gacaatacag cacttgcgcg gtattccgtc 30240
gataccgtcg gcgaagtaga aattaacgcg aagatgattc gccagcgttc gcaacttacc 30300
cgacccggcg caaccgtcgt acttgaacag ttcggcggcg tgccgatttt gacgaagcgt 30360
ccgcttgcga acgaccttgt acgcgacgaa gtttcaaccg gccaaatttc aatcgacaat 30420
caaaccggac ttgccgacat tattagccgt tgggactata gccgcatcgg cgggccgcga 30480
agcttcaaag taaaccgtat ttcgaatccc gaagaattgg attattggaa agcgattctt 30540
gcgtatgcac gcggacaggc gcgcaagttt tggatgccca catatcgaac cgatttggct 30600
ttggccgtgc aaccgtccga cgcgacttca agctataccg tcgaagggtc ggagtatgcc 30660
gaaaagattt ggccgattat cacgcatcgt tatatcgaaa tcgaaacagc gtccggcatt 30720
catcgaaccg aaatattggg cgcaactttg cttgaaactg gcaatacaat tcttcttctt 30780
ggtacgccgc ttccgaccgg ggccggatgg atggaagtta agcgaatttc gtacttgttg 30840
cctgtacgcc ttggcgacga taaagtagaa tggaagcatt acggccttga atcaatcttg 30900
aatctttcaa ttcgaacggc ggaaccctaa tgtctgatta cgacgacaaa gaagaaagcg 30960
taggcgatag cgcgccattt gaactttacg aattcgtcgg ttcatatcgc aattattaca 31020
tgacttccga cgttctggcg cacgtcttca acagtgcgac atataacccc gttccgggtt 31080
tgaagcgcag tacgttaaaa gtcggaacgc acgaagacga caacgtagat atgacggtta 31140
cgcttccgat tacggaacaa atcgttaaag actatgcgtt tcaaactacg ccgccgtctt 31200
tggttttgac gatttaccgt cgccagcgcg acgccgcaac ttgggttgca tactggaaag 31260
ggccgattgc ttcaatttcg gttgacgacg aatttgtaac gtttagaacg ccaagcaagt 31320
tcggttcgat gctgtcgggc aatataccga acgtttacgt acagccgcct tgcaataacg 31380
ttttgttcga cgaacgttgt aaggtaagtc gcgtaagcaa ttcgctagat acgcaggttt 31440
cggcggtcga aggtcgtaac atttcgattc cgtcgcttgg cggttttccc gacggttggt 31500
ttatcggcgg cgaaatcgca atacctgcgc gcaacgaacg tcgaatgatt gtcgcgcaag 31560
ccggggcaat tctaacggta aattacgaat tcgcgcgtat ttctgtcggc acttcggttc 31620
aggttacggc aggttgcgac cattcttaca acggggccaa cggttgcccc aagttcaaca 31680
atcaaccgaa cttcggcggt tgcccgtttg ttccgggcga atcgaataac gtatttcaaa 31740
gcggggttca ttaacaaatg tggattgcaa ttgtcttcgt cgtcgccttc ttacttatgg 31800
cggcgcttat gccgaaaccg aacgtcgaaa acgcacgcgc ggcgaaactt ggcgacttcc 31860
aatttccgcg ttctaagtac ggcgacccaa tgccgctagt gtggggaaca gtgcgccaga 31920
aatcgccgat aacggcttgg tttggcgact tccgcccggt gccaattcgt aagaaaatga 31980
agaccgggct ttttagttcg aagaaagtta cggtcggtta taaaaattat cttggcattg 32040
attgcatttt gtgccttggc cccggcgttc ggcttcgtaa gttttgggcc ggtacttacc 32100
ttgtttggga aggcaactta acttcgtccg gcgatattta cattaacaag ccgaacttgt 32160
ttggcggcga agatgaacgc ggcggcctgc aaggtacaat ttcgttttac gacggtcgtt 32220
ttgacccgcc gcaagattct tacttggttt cgaagattgg cccgaatgtt ccggcttaca 32280
acggctttgc gcgtgcgttg ttcaaagcct tttatatcgg aacaggtacg acgccggaac 32340
cgttcagctt cgaaattcag cgcattacgt ccggcttgca cgcgacttat tcaatcatgc 32400
caaacgggtt ggacgttaac ccgatggaaa ttgtttacga cgcaatgacg caaaaatggg 32460
gccgattcgg caaccttgaa agcgaaattg atatgccttc gttcacggcc tgcgcgcaaa 32520
cgctgtataa cgaaggcttg ggtatgtcgc ttattgttca atctgcgatt accggaaaag 32580
acttgttgga agaagtaatg cgcgtcgccg acggcgtgtt gtatcaagac ccggcaacgg 32640
cgaagattgt tgcgaaactt gttcgccaag attacacaat ttccgaactt cttgttcttg 32700
acgaatcttc cgttaaggaa cttcgcaatt tccagaaaac aacatgggaa aacaccttta 32760
accaatgccg ggttacgttc aaaaaccgcg ccagtagtta cgacgacagc gtagcaatta 32820
cccaagactt cgcaaatatc aactttcaaa accgcgttaa gtctacggaa gtttcgacgc 32880
ccggcgttac cgacgcaacg gtagcaagca agattgcggc gcgtcaactt tcaattttga 32940
acgttccgct ttacaagtgc gacttgaccg ttaaccgcaa ggcgcagaac ttgcggcccg 33000
gaagcgtgtt cgttctgaat tggtcgccgt tcggcatttc gaatatggtt atgcgtgtta 33060
cgaaaattga tttcggcgaa cttacgtcta accaaatcaa gctttcttgc gttcaagacc 33120
gcttttcgtc ttcaacgctt acgttcgcac cgcccgaagg ttcaggatgg acgccaatta 33180
gtacggaacc gacgccggtt acaacgcggc ttctttttac gccgcctgcg ttccttacgt 33240
caaacagcga aaacgaaaca ctttccaatt ttgataacta cggtcggttg tatcttgccg 33300
cagttgcgcc gggttctacg tctatttctt acgacgcaat gacggccccg gacaatttca 33360
gttcgtcgcc tattctgtcg cttgaaagtg ccccgtacaa cggcggcggc gtgttgctga 33420
acgcctacgc ttcgacagtc gccagcgaaa cccgtaacga tacaagcgga agccttgtcg 33480
taaccggcgt gtcgcaagca agcattgaag gtttgcagca atacacgacg ctagaccaag 33540
cgcgcgacgg ttcggccttc ctgctgatta ataacgaact gttcgtttat gtgggcttcg 33600
ttgacaatgg cggcggtcaa gttactttcc cgaacgtgta tcgcggcgtt ctggataccg 33660
tgccgggcaa tcatgcggcg aacgaccgcg tttggttcat tagcggaacc gacggactgt 33720
tgccggaact tgtttcggtc ggaacgacgc gatacgttaa gcttttggat acgacttcgg 33780
gcggcaagct tgatatttcg ttggccccgt cgtttagcgg gtcgcttacc aaccgggccg 33840
ggttgccgct gccgccgcag tacctaacgc ttgcaggaag ccgcacgcca agcccacaag 33900
taggcgcaac gtcgattgtt gcggaatggc gcaaccgcag ccgggccgat accttgttgc 33960
gcgtgtatca cgacacggcg aacacgcggg aagctggcac cgaaacccgc gttcgctggc 34020
gtgtaggcgg cggcgcttac acgaccgtta cgactgccgg aagttcaaca acccttgacg 34080
taacgggcaa gtacggcacg ctagaagtta ttgtagattc gttcattacg tcgaattcga 34140
aatattcgac gtacagcgat tcgcttacaa tgaccctaac ggcaccgccg ccgccgtaat 34200
caaaagaacg acagcgcggc ggttttggcc ttcttcaacc gcccgcagtc gtacaacatt 34260
tcgaccgctt cgttaatgta ccaatcgtaa tttatatcgt cgggaaattc cgtcggcaag 34320
tccattacag ggcgcgcgcc gtcggttttc ccgactttgt ttccgctgga tacgtaagca 34380
atatatccgg cttcgttctt cgggtaatac caacgaacga ccttgccaag ataacgaccg 34440
ttcttttcgc cgccgccgcg cacgttcttt actgcaacga agcgccggaa gtcgcggcat 34500
tccttaattg tcttttcgac cggcgttccg tcaacaagga accgaacaac ggcgtcggaa 34560
caaatcaggg tttcggggtt cttcgaaaga atcgaattca aagccgaccc gcgttcgcaa 34620
taagcgccct tcgtttttgc gcccaactgt tcgtcaataa agcgcgcttc ggggtcgccg 34680
ccgtcttcct tgattgcgac ataactatta acgtcgcggc tgaatacggc tttatatcgc 34740
gtttcttcgg tcttgtatcc tgtccattct tcccaagacg caataagcgc ccttacttcg 34800
tcgtgtcgcg atttgtgata cttggaaata atgccgtcgg tattgccgga aataacttcg 34860
atgccgattt gttccaaagc ttcgataagc ataagcaaca caagttgccc ggtaatcgtt 34920
acttgcaaca taagttgcgg cgcgtaaagc gtcgaatact tgttgccaag cttgccgaag 34980
cttccgttaa tggtaatttt caaactgtcg gcaattgtct tccatttctt cgcggctgcg 35040
cggtcgcccg ccttctttgc ctttgccgct tcggctttgg cgtgaattcg cgtatttacg 35100
attttttcgt aaacggtcaa gaaagcttcg cccaagtgcg gcgggcaaag tctttgatta 35160
aggattgttc gcgggtaaaa cgattctacg tcgttgtctg ccaaaattat ttcgtcggtc 35220
gcatagtgcg ccgttttctt ttcggtagaa tgcaaaccgc ccatgcctag tttgtacgtc 35280
gaaccgccaa tggttacttt cagcttttcc agttcgggcg gcatgattgg cgaccccaag 35340
ccgtcaagat agaagcgggc atttcgcacg acttccagca tttcgcgaag ctgcgtcgtt 35400
tgatagcaaa cgaagtcggg cacgtcgtaa accaagaccg tatcggcgga tagtgtgggc 35460
ttcttcggat aatagccaag gactttttga agttcggagt taataaccgc ttcggctact 35520
tgtgcgtctg atttcgaccg aaggtctacg ccgtattctt ccgacatttc ggcccgcaac 35580
ttcaattcgg gcgcaagttc gttaaaaaga agttcggtat tcgaaaggtc gttgcagcaa 35640
taaggccgca ctatttcggc gtcttcctgc gttaatacat ggtcttcggg aaacggcaag 35700
tcttgcatac gttcgcaatg caagcgcccc gcgtacagct tcaacgaacc ttgaagcggc 35760
gcaacttcta taaggtcgat atgattgtat cggccaattt gtacgccgta cttcttttcg 35820
aacgcgaacg gcgttacctt ttcggttcca tagttcggcc cgcttttaat gatgaaatcg 35880
gacgcttctt taagcttcgc gcagcttgcc ccgcgtgcgg ctagttcaat catcggcaag 35940
tcataattac gcgaattgaa gccgacgata cagaaccgcc aaagcatcca aagaagcttc 36000
gtcgggttaa aatcatggtc ggggctgcgt tcgaacgcga cgaactttcc gttatcaagc 36060
gacttaaacg ctacgtaaaa gaaatttacg taagtttcaa cgtcgaagac gaagacactt 36120
ccggcaggca ttgccgttag ttcttcgtcc gacataaatt cgacaggccg caaagcctgt 36180
cgaatgcttg cggccagctt atcgacgacg cggcttttct ttgcgacgat aaagccttgt 36240
tcgttaatca tgcgaaagcc ccttaaaacg gtatgtcgtc ttcgtaattg tccgacggat 36300
tatgcgtcgc ttcgctgcca aggtcaagac ccataagaac gccgcgcaaa ttgtcgccga 36360
aaaatacgac cttgtttgct tccggtgcga aatgcgcttt cttaaacgca tgttcgacgg 36420
aaagaagcaa cttcgcagaa aagcccattc gttccggcaa gccttcgatt ttgtacgtcg 36480
aagcttcttc tttgtgcatc ttcgacaaaa ccgcgccgtc gtcgaagaat acgtttccgt 36540
tcgggctgaa tgactgaatc gcgcgaacgc cgttgtaaaa atcgtcggga accggccaag 36600
ggttcaaacc gtcaacttcg aaaacgactt ggtaattcgg gtaacgttcg ccgtaaagct 36660
gcgttttgat aaacgccccg ttttcgaaat agaacgttgc cgacgacgcc gaaaagccga 36720
agccggtaag ggccgggcca gccttggcga cagcgaccgc cgcgcacttc ggaagcatca 36780
tgccgggcgg aaggtcgata ccgtgccacg cttccagcaa ggccgccccg ttggtcgcga 36840
cggcgcttcc ggcttgcagc aagacggcgg cataggtcgc attcggtgcg ccgtctgtcg 36900
caagccctgc gaccgcagcg aaggccgttt taatccggtc gtcgattgtg gcgcattgcg 36960
ggtcgggcgc ggtaatcggc acgtcgtcga agcccacaca aggcaccaac gcccggaacg 37020
cgccagacga tacggcaagg gcgttcgcgg taagctgcgt aatcgacagt tcttcgccga 37080
ccttcgacag cgcgtcgata aactgcaacg tatgcgggca cgctaccaag tcttcttcga 37140
taggatgcgc gacggtcaaa accccgtcgg aagccgccgc ccaattgtgg gcaatatgcc 37200
cgaactgaac gttcgtcggc ccggcttttt tctgcgcgat ggcaacgaac ttcaacgccg 37260
ccaaaagcga cgccgcaggg cttggcccct tcgccttttt ctttccggct gcggcttgcc 37320
tgcgcgccct tggctttttc ttcggctgtt cggcggctgg cgcttcgtct tcgggcggaa 37380
gcggaatatt actgtcgatt ttcattttcg gccccttctt cgtcaagttt cgaaattgcc 37440
gcttgaagat agttcgctaa gtctagcgtt tcttctagcg cgtgttgcaa ccaatcgcga 37500
agcgataacg ggttattatc cgtcgttacg ccgtactttg caagtccaac gattgaacgt 37560
tgcaacagta attcgcggtt cttttctacg tttttatcgg gcgatgtttc ggccatttgt 37620
tcaccattca gcggaaagaa cttcgggata ctttttgttc acccatacgc gaacacggcg 37680
cggcgtgcga agttctgaaa cacgctgcaa agcttgataa gtcgtcggcg gcggttcttc 37740
ggcgtgccgc tgccgccacc aatcgcgcga acgtttcgca gcaagccccg gatgttcaag 37800
cattataaat tcgttgaaca tttggaagcc gcaaaagtaa ctaaccttaa tcatcggcgg 37860
tttcgttaga tttccgtttg cgtcgcgctt ttcgtgcaaa ttgtaaagta ccttttgaac 37920
gtcgaaatat tcgactatcg gcgcgtcgct tcgcagcggt tccagcgttc cggcagttgc 37980
aaacaacttc gtttcaaaag taaattcggt gccgcagttg atacactgcc gggccgcagc 38040
gtgattatat acgccgcata cgtcgcaaat acgaacgggc gcgtcgccgc cgcctttgcc 38100
cggtctgttc ggaattcgcg ggtcgttgat tgggccaagc cttcgaacgt ttccggcaaa 38160
gtccgcgtac aggcaatttt gtttgccagt atccggcgac ggtcgggttc cgcgtcccaa 38220
cttttgaacg tgtttgcccg gcgatagcgt cgggttcaaa tcgccgataa agtcgattgg 38280
cggatggtcg aaacccgttg taagtttctg ccctgatacc aagccgcgaa gttcgccagc 38340
tttgaaagcg cgcaagcgtt cggtattggt cttcgaaggc aacttggaat gaaccggcaa 38400
aacttccaag ccgtaagact gaattacgtt cgcgacgtgt tcggtattat caatgccagt 38460
tgcgaagata agccaagtcg aacggtcgta agctatttcg gtcatttccc gaacagcgtt 38520
aaaaacaact tcgtcggtat ctacggcggc ttctagttgc ttgctgttga attcgccagc 38580
cgttacgcct actttcgaca agtcgatttc ggttcgcgtc ggccttgcga taagcggcga 38640
caagtagcct tcggcgataa gtcggttaaa cgattcgacg ccggttatat cgtaacaaat 38700
gtcggtaaaa attccgttgt cggtaataag ccccattttc attcgatagg gcgttgccgt 38760
aaatccaata actttaagat gcgggttaat cttgcgaagt tcggcgataa tgtattggta 38820
atgcgaatct tccttgtcgc ttaacaaatg gcattcgtcg attaacagca agtcgcgcca 38880
accgaaatgg cgaagatgcg ccgggcgtcc gtcgtcttct tcaagcccgc gtttaatcgc 38940
aggcgctacg gactgaacgc cgccgaatac gattggcata atcatatcgc gggaattcaa 39000
gcccgccgaa taaatgccca ttggcgcggt cggccaaatc gacataagct tttcggcgtt 39060
ctgttcgatt agttctttaa cgtgcgtaag catcattatt cgttgattcg gccagtaacc 39120
gaaaattcga cgaataaagt ttgcaattac gaccgacttt ccggttcctg tcggcatggc 39180
gacgacgggg tttccgaccc cgccgcgctg gaaataatcg aatattgaat attccgcttc 39240
gtcttgatac caacgcggaa cataaatatt agacattctt tgcaaccgct gtaattggcg 39300
aataagaatc gcaacccgta gcgatgaaat cgcccggaat tacgccgttg tgaacttcgc 39360
aaaaccattc gccgttttcg acaggatttg cggccttgca gcttcggcag ttcttttcag 39420
gaatcgcgcc cttgtggcaa atatccttcg ccgcgcaata tccgcacttg tgaaacgtcg 39480
gattatccga caagcgcggc ggcgcttcct gcgacagaat gatttgttcg gccttctcct 39540
tcatttgctg gcctagccga tggtctagct ttacaacttc gcaatgcaag tcgtcgtcgt 39600
tcttgttaat gttcaggtaa agaacataac gaaaacgata ttcgttgcca tacgtcgaag 39660
tttgggcgaa atgctgcggc ttgcttactg ccattccttg cgacattaag tccgaaaagc 39720
ctttgccggt tccgttggtc ttgaattcca gcaaaacggg ttcgtcgata ccgtagcgtt 39780
cgggaagccg ggcgatgccg tcaagcgacc cgccgaagtg ccccataacg ccggaaatgc 39840
ggtattgtgg gaattccaag ccgtcggctt tcgcgcgtgc gacgtgttgg gcgtaatacg 39900
gattttcgtt cgtaataagg tcgcagccca acggcagttc ttcgccttcc ttcaatatgc 39960
cgtaactgtc gctttcggct tggtaaaaga aaccgtcgcg gttttcgtac caacattgaa 40020
cgccgatgcc ttccagccat tcgataaagc gggcttcttc acggtgccca cgattaaaca 40080
agcgttgctt gcgcccgtcg gtttgttcgc ggaaacacca acggaagata taccaaagct 40140
tccgcttgca ttcgtcgcca atcaaagacg cgccaaggtg ccgacggtgc ccgccgtcat 40200
acgtgcgaac gcaatattcg tctatatctt ctttaatgcg cttcgcaagg gcggttgcaa 40260
cgccgggcgc gtcaaggtct acgctttgtt ttcctgcgtc ggatttcgtc ggctgcgcgt 40320
cgttcagcgt taattgtgcg ttcttcgcgt ctttgctgtt ttgcatcgtt cttcaccttg 40380
cgccgcataa gggcttcgta attgttcgct agataatccg ccgaaagtgt cgcaacgtct 40440
ttaagttggt ctttcgacag ccaagatata tggcattccg acgggtcgat tcctaattgg 40500
ttcgcaagcc aattgtacgc cttcgcgcgc gacattaagc cggtttgcca aaggcggtcg 40560
aattcgttat gggctttcgt tcgaagctgg cgcgttgttc tgtcggccat acgacccaac 40620
ggaataaacg tgcccggatg gcaaccaacg gcggcgcgac aatcattgca ataataaatg 40680
tgcggccaat tgccataagt tcgcccgtaa atcttgtcgt tcgttgttag ttcaatgttg 40740
aacgaacaac aagtatcgca ttgttcgggc gtcggcaatg cgtcttttat tttcgacata 40800
agttcgcata ctccttaaaa agaaacgccg gggccggtta agaccccggc gaatctacgc 40860
cgcgacggcg ttacgcctta acgctggccc caaggtgccg cgcctgccgg tgcgccgccc 40920
tgcgcgggct ggccgccgcc ctgttgccag ccgccgccct gcggttgctg ctgcgcgggc 40980
tgttgggcag gctggccgcc ccatgccggt tggccgccct gcggggccgg gttctgcgcc 41040
ggttggccgc cccaagctgc gccgccctgc ggctgttggg ccggttgctg cgcgggctgc 41100
tgctgtccgc cccaagcggc accgcctgcg ggctgctgcg gctggccctg cggggcaccg 41160
ccccaagcgc cgttcggctg gccctgcggc tgctgttgct gcggctgcgc tgccgctgcg 41220
cctgcgccag ccttgcccgg ttcgttcccg ttcatatcga agaccttttt aacttcggta 41280
tactgcgggt cgtttttctg cgggccgact tcgataagaa acggcaggtt gtgaagctgc 41340
gacgaatcgg taacttggaa cacgccgatt acgtggcaga ttgccgaaag ctggcgatgc 41400
gcgatttcga cggtctgttg gttggaatgg tacaggttca ggcgataagc gccggtcgtg 41460
ccctgctgcg ggccgtcgat aatccgcaag ttcagttgca gataaccgcc gtcgttcgcc 41520
ttgttggcct tgacttcgga agattcgacg ataaccggat gcttgccaat cggcaggctt 41580
ccgacgcctt gggtcgggtc gtactgttgg gcgttgaacg cttggataag ttgcatgatg 41640
tttcaccttt tcgaaaagtc ttcgccattg gttacggcgg cgaagtaaac cgttttattg 41700
catcgctttc gcgaacaatg cggtaaggtc gggctgttca agttcgttaa gattgcccaa 41760
acggtcgcgc gcgaaaactt cgggaatttc cttcgttcgc agcgcccgaa ccggcttcgg 41820
cattccgggt acgcttgctt cgcccaagtg cataacgtta tcgaacaaat gcggaacttt 41880
aacgttaagg tctttgcccg gaaagaacgg gcgtttttgc ataatcggtt cgtaagtaac 41940
ttcgccgttc tgcaaaatcg tttgccgtcc gttttcgaca agggcttgct ttgcaatcat 42000
tacgatatgc ttttgcggca tgtaatacaa gtcgttcgcg attttcatta cacgttcgga 42060
catattgccg taagccttca taccgtgttt aaccttgccc aattcgtcgg ccaagataat 42120
ttcggcgatg ttcgaaatac tgtcaatacc caacgtatcg aaattcgcag cttcgcgcga 42180
tttcataaac cattcgaaga attcgacgat aagcgcgggc gaatacgctt cccatgcggg 42240
aacgttcgaa ccgcgcatgg acagcatacc gggttcggtc acaagcaaaa ccggacgcgg 42300
cgcagtattg ataagcggcg ttttgccgga accgggcgca ccgaatacga cggactttac 42360
gccgtagcgg cgggccagtt gcgacgccgg ttttaattgc gacatttgca taacttgcac 42420
cttcgggaat ggccccggcg cttgtgggca accggggcgg ggttgttact tcgacttcgg 42480
cgcgacaagt tcaagcgcgg gcgttgcttc cttcgacgtt acaacttcgt cgataagctt 42540
acggaacttg tcgggaaggt tcttgtattc ggtaacggaa agttcgggct tccattttac 42600
caaacgttcg gcgataaatt cgccttcggg gccggtcttt tcgattttct gcaaagcctt 42660
ttcgacggct tcttggccgc ctgcgaagct gcggttaatc ttaaaaacgg ctttcagctt 42720
gtaacctgcg ccaagttcga cgttttcggt tccttcgcgc aaagcttcgg ggtcgaaagc 42780
gaaagcgttt ttcagaactt cggcgcgcaa tgcggattcg gcttctttcg ctgcggcaag 42840
cgccttcgtt gcttcctgcc attgcagaat caaagcgtcg cggttagcgt tttcgttgaa 42900
agacattgtt gttactcctt cggttgggcc gc 42932
<210> 16
<211> 42932
<212> DNA
<213> Artificial Sequence
<220>
<223> s4 phage变体
<400> 16
ttgcggtaaa ctcaaagggc cgggtaatac cggccctttc ttcattcaat cggagtaaat 60
caaatgtctg gactgtttaa gcaattcaaa acgaattcga ccaaggaagt cgaaggcgtc 120
gaaatcgaat ttcccgaagc gcagaacgac gacggcagcg ttccgacttt cgttatttcc 180
cgcatgggca aatcgaacaa ggcgtattca aaggcgcttg acgcggcgac ccgtccttat 240
cgccgtcaag tcgaacttgg cacgctgaaa aacgaagtcg ccgaatcgct gtttatgggc 300
gtgttcgtcg ataccgtgtt gcgcggctgg aaaaacgttc agggcgaaga cggcaaggaa 360
attgcgtatt cgaaagacgc cgcaatttcg cttctttccg aactgccgga cgtttacgaa 420
cgtttgcagg aagaagccaa gttggcttcg aacttccgcg ataacgcttt ggaagccgaa 480
gcaaaaaact aaccgaagtt ttggcgcacc tgttggaact tggcccacac gaacaggcga 540
tagcaaaaca agcgatgcgc gcggggcagc cgttacccga acgcatcgcg aacgcgccag 600
aacttgaagt aggcttgcag ttgtatttgc aagccttctt cgacctagat agcgaacggt 660
cgcacggcaa cggtttaacg ccgatacctt ggacaagtat ggcggcttac gcaagggctt 720
tcgagtttga cgaagaacaa accgaagact tgttttactt tatgcgaaag cttgattcgg 780
aacacttgaa gaaactagcg gataaacaga aagcggcggc atcgaatggc aaaaagcctg 840
ctagacctag ccgataggct ggaaaagaaa gcgaaggcaa tagacgaagc ggcgtcacaa 900
aacgccgtcg ataccgcttt ggctattgtg ggcgacttgg cgtacaaaac gcccgtagat 960
acttcgcaag ctttgtcgaa ctggattgta acgctagaaa gtccgtcggg tcaacaaatc 1020
aagccgcatt ttccgggttc gcagggttca acgcaacgcg cttcggcggc tgaaacgttg 1080
aattctgcga agcttgtttt acgaaacaag aagcccggcc aagcgatatt tattacgaac 1140
aaccttccgt atatccgcag gctgaacgac ggttattcgg cacaagcccc ggcggggttc 1200
gtcgaacgcg ccgttttgat tggtcgcaaa atgcgtaaga agttcaagat taaggattaa 1260
aagaaatggc cgacgaaaac atagaaatta aggttcaaga caaagtttcg ccgtccattt 1320
cgacgaagct tcgcacaatt gcaagcgaag cccgcaacgc cgacgccgca gttaagaacc 1380
ttcaaacgca gctttcggca attaacgtcg gcggcttgtc gcagcttatc aacgcatcgg 1440
ccagcgcaac gcgccagctt caacaaggcg cgcttgccgc gcaacggctg gcaaccgaac 1500
agcagcgcac cgcaacagcc gcagcgcagg ccgccgcagc ccaacagcgc gtcgcgacgg 1560
ctgcgaccca aggggcaacc gcgcaagcca accttgccag cgccacgcag cgcacgcaga 1620
cggcccaaca gcagacggcg acagccgcgc agcgcctagc gacagaacag cagcggacag 1680
ccgttcagac ggccaacgcg gcggcagcga acgaccgggc cgcccttgcc gccctgcggc 1740
tgcaacaggc gcaagaccgg gccgccaatt cgacccgcaa tgcgacttcg gcccttggcg 1800
gatacattcg aaccgctgcc ggaattcttg gcgtaacgat ttcggcgaac gcaattcttg 1860
caagcgccga cgcatacgtt acgcttcaaa acaagttgca gaacgtaacg aagtcgcaag 1920
aacaagttaa cacgcttacg aacgaacttt tcgaactgtc gaaccgcacg cgcgcgggta 1980
ttgaagaaac ggcgacagcg tttacacgct tcgaccgtgc cttggcgttt atgggtaagt 2040
cgcaagaaga ttcgttgcga atgaccgaaa ccattaacaa ggctttgatt gtttccggtg 2100
caacagcgca agaagcgtcg tcggctttgt tgcagctttc gcagggcttc aacgccggta 2160
agctgcaagg cgacgaattc cgcgccgttt cggaaaatat gccaatcgtt ttggacgcag 2220
tagcgaaggc gcttaacgtt ccgattaacc gcgttaaaga actttcgacc gaaggtaaga 2280
ttacttccga agttctgttt gaagctttca agcttataca agaccaagta gacgcaacgt 2340
ttaacaagac gacgcccaca atcggccaaa gcttgacggt tcttcgcaac aatgcaattc 2400
agttcttcgg cgaattgaac aaagcgaccg gctttactgc cgggctttcg cgcgcgatta 2460
tttggcttgg cgaaaacttg aagacggttg ccgtaattgt cgccgggctt ggcgtcgcat 2520
tgcttgtcgc cttcggtgcc ccgttggttg gtgcgctggc cggtgcgacg gctgcggtaa 2580
aagcctttac gctggcgctt gcttcgaacc cgattggcct tatcgtcgtc gcgctttcga 2640
ccgcgattgc gtatctaacg cttttccgcg acgaaattaa cttgggaatc gacgacgtta 2700
cgacgttggg cgatttcttc cgcgcgacct ttgaaggcat cggccaagca atcgacgacg 2760
ttaaaaccat cgcgtcgcaa ttgtgggcag aacttagcga cttcgcaagc gcagcccttg 2820
gcgaaattac ttcgaacgtt gacgattcga cttcttcttg gtcggacagt tacgccgaat 2880
ttttccaaac caaccgtaca ggttgggccg gtgcgttgga aaacgccgca aaagttcttg 2940
acgcaatcgc cgggcttttg accggggccg caacgttcgc aggccgtgca atggccgaag 3000
ttgttatttc ggttcaaaac ggaatcgcaa acgcttacaa cgttgtcgcg ggttggattg 3060
aaaacgtaac gaataaagcc attgaagccg cgaacaggct tcgcgcgatg gttggtaagt 3120
cggcttacga acttgtccaa ttcgaacgca tgggcagcgc aggccaaacc gaattcgaat 3180
cttggggcaa gctttgggcg caatcgctgg aagacggatt taacagccaa ggcggcgcaa 3240
tgcaaaacct gttgaacggc cttttcgacc gttcgcagca aatcggcgcg cagcgcaggg 3300
ccgcaggaag cgcgcagctt cgcggggccg gtacgtcgca gcttgccggg gctaccgacg 3360
caaacgcggc gaaggctgcg gaacgtcgcg cgctggcgtt ggaaaaaatc aatacgcagc 3420
ttgataacga attggcgcgc atgttccagc ttcaaccgca gcgcgaagcg caagcgaagt 3480
tcgaccaaat cgaagaaagc ttgattcaga aaaagattaa gcttacgtcg gaagaagccg 3540
aagcaatcaa ggcgaagatt aaggccgttc aagacgcgac cgaagttcaa cgccagttcg 3600
acgcaattta cgccgaagct gttaaccctt tgaaggagta caacgcggcg caggaagcgg 3660
cgaacaagct tcttcaaatg ggcgcgatta cgcaggaaca gcacgcccgc gcagtaacga 3720
aggcgtcgga agcttacgcg aattcgcaag acccgttgcg gcagtataac cgcgaccttg 3780
aacagcaatt gcagcttttg caaatgctgc cgaagcaacg cgaaatcgaa cagcaggtta 3840
tgcaagttca gaacgatttg cttgcaaagg gcatcgttct aaacgaaacc gaattggcgc 3900
agcttcgcga aaagctgttg cttattcagc aagtaaacgc cgtttcgcag caagaagcgt 3960
cgttgttgga cgccagcgta aacaagcgcc aacagtttat cgaccaactt aaagcaattc 4020
agaacttgcg caacaacagc ggaagcggtt tcaacgcagg cgaccaagcc gaagcaacta 4080
acagcatgtt gcaaggcatg ggcattgata cgacgaactt tcaaacgcag cttaacgcgc 4140
aattggcgca atatcaaact tacgtcgaac agcttaaaat gctgaacgaa cagcgtttga 4200
ttagcgacca agaatacgcc gccgcaagta tgcaacttga attgcagcgg caaaacctgt 4260
acttgaattc ggcaagtagt ttcttcggaa acttggccgc gcttcaacag tcgggaaata 4320
agaaaatggc ggcggtcggg aaggccgcag caatcgcgca agcgatgatt aatacgtatc 4380
aatcggcaac gtcggcttat gctgcgatgg cgtcgattcc ttacgtcggc ccggcgttgg 4440
gcgcggctgc ggctgcggct gcaattgccg caggcttggc gaacgttcag caaatccggt 4500
cgcagaatac cgggtttaag tcgggcggtt ttaccgggtc aatgggcgta aacgaagtcg 4560
cgggcgtcgt acacggccaa gaattcgtta tgaacgcagc ggcgaccaac cgtattggcg 4620
ttgcagacct tcaagccctg caaacgggcg cggccagtgt tcagcgcaac gacgaacagg 4680
caatggcggc tagtgtgggc aggggcggca attccgaacc cgcgcccgcg ccggttgtca 4740
atgtaccttt tagcgccgtt gtcgtacaat cgaaagaagc ggccttggcc gggcttaaat 4800
ctgcggaagg tcgggctttc attctggaaa ctatcgaaca aaacggcggc accgtcgcaa 4860
aaatcgtcgg agttaaataa atgggttacg caattggaac agttacaaaa ggcggcggcg 4920
acgattgcca ttatcaagtt ttggcaatta tcaaaacgct ggccgaagcg aacggatgga 4980
cgacgcttcg ttacgtatca accggacttg accgcgaatt gattttgaaa ggcgttggtt 5040
tgtccggtct tgaagaaatc tttgtagggt ttaagtgtta tcaaagcgtc ggcggcgatt 5100
attacaatat taattgcgga actttcgtcg gttacgtaag cggaaacact ttcgaaacgc 5160
aacccggcgc gcagtttagc gcaacgcctg cccacaataa cgcgattacg tatttcataa 5220
cggccaacgc ccaacgaatc gtcggatgct tcaaagtcgg tacgccggtt tacgaacatt 5280
tttacgtcgg caagttcttt ccgtatgcgc ggcccggtga atatccgtcg ccgcttgttt 5340
gcggtgcgat gctttccaac gcgcaggcaa tgcgattcga caacaacgcg cagtcgttcc 5400
cgtatccggg ctattattac gacacgacga acaaccgcct ttatattcgc gaccaagcgg 5460
gcaactggat tcgcccttgg tgttggccat ttaccaatgg aaacggcgca aacaccgcgc 5520
ttgcagggcc gcaaggaact tcgacgcttg ttcctgccga cgtgtattac caacttgaac 5580
caattatttt ggcgcagcaa agcaacaata acaacccttc gaacgtatgg ggcgaattcg 5640
acggcgttta tttctgttcc ggctttaaca acggcgtcga aaacgtagtt caggaaggcg 5700
gaagttctgt cgttgaccaa accgggctta ccgtacttca agccgtagac gctattaagg 5760
cggtcggcgg gcgggcgttt gtaatgttgc aaaacgtcta tcgtacttct tggcgcgatt 5820
ttatcgcttt ggaaatgaaa taatggcata cattacagga accgccgcaa actttgcgga 5880
tttgaaaaca gccattgaaa acgcctgtac ttctaacggt tgggcgcttg ccgacggcat 5940
attgtcgaag aacggttgtt tctttcaact aatcgccgat agtttgggcg gttatccgca 6000
acttcgcttg catggcggaa caggcaaaag cggttcgacg cttacaggcc agccgcctag 6060
caattacggg gcaaaggtcg caagccctgt cggaaatcaa atcgtattcc cgattaatta 6120
cgaaatacat ttgtttaccg accccgacga agtttattgt atcattaatt acaattcgga 6180
cttttaccaa caactttcgt tcggcaagtc tgatattccg ggcattggtg gaactggcgc 6240
atggtttacc ggggcatatc ggcacgacta cggattgacc gacgcaactt acgcccatag 6300
ggtttttacg gcgatttctt ggcccgaaat aactggcgtc gcaacttatg ccggaatgtg 6360
cgtcggtttg ttttttgaag gcaacgcttc gcaaactggc ggaagttatt tcgcaagctt 6420
cgtacatacc gggcttgatt cgacgccttg gcgaagcggc cacggcggcg acacgctgtt 6480
aagcggctat cgcggggccg gtctttccgt cggttcgctt ttaatgtcac tgccgaatat 6540
ttcgaaccaa gcaacgattt tgcttcctat taagccggtt gcgcgtcgtt caagcggcgg 6600
cgcaactatt atcgcaaacc cgaaaaacgc gcgttatttg cgaatcgaca acgttgtacc 6660
gggcgaaatt attacattcg gtgccgacca atggaaggtt tacccgtttt accgcaaaga 6720
cgcaaccgtt cgaaacggcg tcggttggtc aagcggcgcg acgcatagcg gaacgttcgg 6780
atacgccatt aaatacactg gaacttaaaa agaatggccg gaagaattgg aacattttta 6840
gcggcggaaa gttttattta cggcgattat aacccgaacc tttccgccga acttaacgaa 6900
cttaccgccg cagaatacgc gccgacgatt tacgacccgc gcgatatgtt cttgcgcggc 6960
ggatattcgg cgtacaaaat cgcgccttcg aatcgctggc ctattgtggg caaagaacaa 7020
ggcaacttcg ggcgcgacta ttattatcgc gttcacgttt cgccattggt cttggacttg 7080
caaacggtcg cgtcgtcgca aacgcgcgaa ttcaacgttt ggaacgcatg gcccgaccgt 7140
gcggctaatc ttgacgacat tttggtaagt aatccggtcg gtattgaaat tacaggccaa 7200
gcgacgccgt atgcaatgcc gccgcttcaa gaacttactt acgaaataac cgtaggcgtt 7260
tcggggccgc cgaatattaa cgttgaagtt caatttgact tttccaacgt tcccgacccg 7320
ctgccgattc tgattacagg aacgcgcgcg gttaagttcg acattgtgcc cgaagttcca 7380
ttgaacgaaa cttgggaatg gttaagcgac aacattgtag cggttgacgg aacagaacag 7440
cgcatagcac ttcgcggcga aatgccgcgt attgaagaac gcttgaaagt aatttttgat 7500
tctgtcgaag ccgtgcgccg tttttacggc gacgttatgt cggcagttgg tcggctttgg 7560
attcccgaat ttcagtacgc gacccgaatt actgccgcaa gcaatagcgg cgcgcttcaa 7620
atttattttg accgaacgaa gaccgacgtt cgcgataccg aatacgtctt gattcaaacg 7680
ccgcttgttg cggcacttgt cgaaatcgac gtattgacag ccgacggggc tacgcttggt 7740
tcgccgctgt tgttcgacat tccggcaggg tcgttaatca tgccgggttc gcccgcgctt 7800
attgacgaca atacagcact tgcgcggtat tccgtcgata ccgtcggcga agtagaaatt 7860
aacgcgaaga tgattcgcca gcgttcgcaa cttacccgac ccggcgcaac cgtcgtactt 7920
gaacagttcg gcggcgtgcc gattttgacg aagcgtccgc ttgcgaacga ccttgtacgc 7980
gacgaagttt caaccggcca aatttcaatc gacaatcaaa ccggacttgc cgacattatt 8040
agccgttggg actatagccg catcggcggg ccgcgaagct tcaaagtaaa ccgtatttcg 8100
aatcccgaag aattggatta ttggaaagcg attcttgcgt atgcacgcgg acaggcgcgc 8160
aagttttgga tgcccacata tcgaaccgat ttggctttgg ccgtgcaacc gtccgacgcg 8220
acttcaagct ataccgtcga agggtcggag tatgccgaaa agatttggcc gattatcacg 8280
catcgttata tcgaaatcga aacagcgtcc ggcattcatc gaaccgaaat attgggcgca 8340
actttgcttg aaactggcaa tacaattctt cttcttggta cgccgcttcc gaccggggcc 8400
ggatggatgg aagttaagcg aatttcgtac ttgttgcctg tacgccttgg cgacgataaa 8460
gtagaatgga agcattacgg ccttgaatca atcttgaatc tttcaattcg aacggcggaa 8520
ccctaatgtc tgattacgac gacaaagaag aaagcgtagg cgatagcgcg ccatttgaac 8580
tttacgaatt cgtcggttca tatcgcaatt attacatgac ttccgacgtt ctggcgcacg 8640
tcttcaacag tgcgacatat aaccccgttc cgggtttgaa gcgcagtacg ttaaaagtcg 8700
gaacgcacga agacgacaac gtagatatga cggttacgct tccgattacg gaacaaatcg 8760
ttaaagacta tgcgtttcaa actacgccgc cgtctttggt tttgacgatt taccgtcgcc 8820
agcgcgacgc cgcaacttgg gttgcatact ggaaagggcc gattgcttca atttcggttg 8880
acgacgaatt tgtaacgttt agaacgccaa gcaagttcgg ttcgatgctg tcgggcaata 8940
taccgaacgt ttacgtacag ccgccttgca ataacgtttt gttcgacgaa cgttgtaagg 9000
taagtcgcgt aagcaattcg ctagatacgc aggtttcggc ggtcgaaggt cgtaacattt 9060
cgattccgtc gcttggcggt tttcccgacg gttggtttat cggcggcgaa atcgcaatac 9120
ctgcgcgcaa cgaacgtcga atgattgtcg cgcaagccgg ggcaattcta acggtaaatt 9180
acgaattcgc gcgtatttct gtcggcactt cggttcaggt tacggcaggt tgcgaccatt 9240
cttacaacgg ggccaacggt tgccccaagt tcaacaatca accgaacttc ggcggttgcc 9300
cgtttgttcc gggcgaatcg aataacgtat ttcaaagcgg ggttcattaa caaatgtgga 9360
ttgcaattgt cttcgtcgtc gccttcttac ttatggcggc gcttatgccg aaaccgaacg 9420
tcgaaaacgc acgcgcggcg aaacttggcg acttccaatt tccgcgttct aagtacggcg 9480
acccaatgcc gctagtgtgg ggaacagtgc gccagaaatc gccgataacg gcttggtttg 9540
gcgacttccg cccggtgcca attcgtaaga aaatgaagac cgggcttttt agttcgaaga 9600
aagttacggt cggttataaa aattatcttg gcattgattg cattttgtgc cttggccccg 9660
gcgttcggct tcgtaagttt tgggccggta cttaccttgt ttgggaaggc aacttaactt 9720
cgtccggcga tatttacatt aacaagccga acttgtttgg cggcgaagat gaacgcggcg 9780
gcctgcaagg tacaatttcg ttttacgacg gtcgttttga cccgccgcaa gattcttact 9840
tggtttcgaa gattggcccg aatgttccgg cttacaacgg ctttgcgcgt gcgttgttca 9900
aagcctttta tatcggaaca ggtacgacgc cggaaccgtt cagcttcgaa attcagcgca 9960
ttacgtccgg cttgcacgcg acttattcaa tcatgccaaa cgggttggac gttaacccga 10020
tggaaattgt ttacgacgca atgacgcaaa aatggggccg attcggcaac cttgaaagcg 10080
aaattgatat gccttcgttc acggcctgcg cgcaaacgct gtataacgaa ggcttgggta 10140
tgtcgcttat tgttcaatct gcgattaccg gaaaagactt gttggaagaa gtaatgcgcg 10200
tcgccgacgg cgtgttgtat caagacccgg caacggcgaa gattgttgcg aaacttgttc 10260
gccaagatta cacaatttcc gaacttcttg ttcttgacga atcttccgtt aaggaacttc 10320
gcaatttcca gaaaacaaca tgggaaaaca cctttaacca atgccgggtt acgttcaaaa 10380
accgcgccag tagttacgac gacagcgtag caattaccca agacttcgca aatatcaact 10440
ttcaaaaccg cgttaagtct acggaagttt cgacgcccgg cgttaccgac gcaacggtag 10500
caagcaagat tgcggcgcgt caactttcaa ttttgaacgt tccgctttac aagtgcgact 10560
tgaccgttaa ccgcaaggcg cagaacttgc ggcccggaag cgtgttcgtt ctgaattggt 10620
cgccgttcgg catttcgaat atggttatgc gtgttacgaa aattgatttc ggcgaactta 10680
cgtctaacca aatcaagctt tcttgcgttc aagaccgctt ttcgtcttca acgcttacgt 10740
tcgcaccgcc cgaaggttca ggatggacgc caattagtac ggaaccgacg ccggttacaa 10800
cgcggcttct ttttacgccg cctgcgttcc ttacgtcaaa cagcgaaaac gaaacacttt 10860
ccaattttga taactacggt cggttgtatc ttgccgcagt tgcgccgggt tctacgtcta 10920
tttcttacga cgcaatgacg gccccggaca atttcagttc gtcgcctatt ctgtcgcttg 10980
aaagtgcccc gtacaacggc ggcggcgtgt tgctgaacgc ctacgcttcg acagtcgcca 11040
gcgaaacccg taacgataca agcggaagcc ttgtcgtaac cggcgtgtcg caagcaagca 11100
ttgaaggttt gcagcaatac acgacgctag accaagcgcg cgacggttcg gccttcctgc 11160
tgattaataa cgaactgttc gtttatgtgg gcttcgttga caatggcggc ggtcaagtta 11220
ctttcccgaa cgtgtatcgc ggcgttctgg ataccgtgcc gggcaatcat gcggcgaacg 11280
accgcgtttg gttcattagc ggaaccgacg gactgttgcc ggaacttgtt tcggtcggaa 11340
cgacgcgata cgttaagctt ttggatacga cttcgggcgg caagcttgat atttcgttgg 11400
ccccgtcgtt tagcgggtcg cttaccaacc gggccgggtt gccgctgccg ccgcagtacc 11460
taacgcttgc aggaagccgc acgccaagcc cacaagtagg cgcaacgtcg attgttgcgg 11520
aatggcgcaa ccgcagccgg gccgatacct tgttgcgcgt gtatcacgac acggcgaaca 11580
cgcgggaagc tggcaccgaa acccgcgttc gctggcgtgt aggcggcggc gcttacacga 11640
ccgttacgac tgccggaagt tcaacaaccc ttgacgtaac gggcaagtac ggcacgctag 11700
aagttattgt agattcgttc attacgtcga attcgaaata ttcgacgtac agcgattcgc 11760
ttacaatgac cctaacggca ccgccgccgc cgtaatcaaa agaacgacag cgcggcggtt 11820
ttggccttct tcaaccgccc gcagtcgtac aacatttcga ccgcttcgtt aatgtaccaa 11880
tcgtaattta tatcgtcggg aaattccgtc ggcaagtcca ttacagggcg cgcgccgtcg 11940
gttttcccga ctttgtttcc gctggatacg taagcaatat atccggcttc gttcttcggg 12000
taataccaac gaacgacctt gccaagataa cgaccgttct tttcgccgcc gccgcgcacg 12060
ttctttactg caacgaagcg ccggaagtcg cggcattcct taattgtctt ttcgaccggc 12120
gttccgtcaa caaggaaccg aacaacggcg tcggaacaaa tcagggtttc ggggttcttc 12180
gaaagaatcg aattcaaagc cgacccgcgt tcgcaataag cgcccttcgt ttttgcgccc 12240
aactgttcgt caataaagcg cgcttcgggg tcgccgccgt cttccttgat tgcgacataa 12300
ctattaacgt cgcggctgaa tacggcttta tatcgcgttt cttcggtctt gtatcctgtc 12360
cattcttccc aagacgcaat aagcgccctt acttcgtcgt gtcgcgattt gtgatacttg 12420
gaaataatgc cgtcggtatt gccggaaata acttcgatgc cgatttgttc caaagcttcg 12480
ataagcataa gcaacacaag ttgcccggta atcgttactt gcaacataag ttgcggcgcg 12540
taaagcgtcg aatacttgtt gccaagcttg ccgaagcttc cgttaatggt aattttcaaa 12600
ctgtcggcaa ttgtcttcca tttcttcgcg gctgcgcggt cgcccgcctt ctttgccttt 12660
gccgcttcgg ctttggcgtg aattcgcgta tttacgattt tttcgtaaac ggtcaagaaa 12720
gcttcgccca agtgcggcgg gcaaagtctt tgattaagga ttgttcgcgg gtaaaacgat 12780
tctacgtcgt tgtctgccaa aattatttcg tcggtcgcat agtgcgccgt tttcttttcg 12840
gtagaatgca aaccgcccat gcctagtttg tacgtcgaac cgccaatggt tactttcagc 12900
ttttccagtt cgggcggcat gattggcgac cccaagccgt caagatagaa gcgggcattt 12960
cgcacgactt ccagcatttc gcgaagctgc gtcgtttgat agcaaacgaa gtcgggcacg 13020
tcgtaaacca agaccgtatc ggcggatagt gtgggcttct tcggataata gccaaggact 13080
ttttgaagtt cggagttaat aaccgcttcg gctacttgtg cgtctgattt cgaccgaagg 13140
tctacgccgt attcttccga catttcggcc cgcaacttca attcgggcgc aagttcgtta 13200
aaaagaagtt cggtattcga aaggtcgttg cagcaataag gccgcactat ttcggcgtct 13260
tcctgcgtta atacatggtc ttcgggaaac ggcaagtctt gcatacgttc gcaatgcaag 13320
cgccccgcgt acagcttcaa cgaaccttga agcggcgcaa cttctataag gtcgatatga 13380
ttgtatcggc caatttgtac gccgtacttc ttttcgaacg cgaacggcgt taccttttcg 13440
gttccatagt tcggcccgct tttaatgatg aaatcggacg cttctttaag cttcgcgcag 13500
cttgccccgc gtgcggctag ttcaatcatc ggcaagtcat aattacgcga attgaagccg 13560
acgatacaga accgccaaag catccaaaga agcttcgtcg ggttaaaatc atggtcgggg 13620
ctgcgttcga acgcgacgaa ctttccgtta tcaagcgact taaacgctac gtaaaagaaa 13680
tttacgtaag tttcaacgtc gaagacgaag acacttccgg caggcattgc cgttagttct 13740
tcgtccgaca taaattcgac aggccgcaaa gcctgtcgaa tgcttgcggc cagcttatcg 13800
acgacgcggc ttttctttgc gacgataaag ccttgttcgt taatcatgcg aaagcccctt 13860
aaaacggtat gtcgtcttcg taattgtccg acggattatg cgtcgcttcg ctgccaaggt 13920
caagacccat aagaacgccg cgcaaattgt cgccgaaaaa tacgaccttg tttgcttccg 13980
gtgcgaaatg cgctttctta aacgcatgtt cgacggaaag aagcaacttc gcagaaaagc 14040
ccattcgttc cggcaagcct tcgattttgt acgtcgaagc ttcttctttg tgcatcttcg 14100
acaaaaccgc gccgtcgtcg aagaatacgt ttccgttcgg gctgaatgac tgaatcgcgc 14160
gaacgccgtt gtaaaaatcg tcgggaaccg gccaagggtt caaaccgtca acttcgaaaa 14220
cgacttggta attcgggtaa cgttcgccgt aaagctgcgt tttgataaac gccccgtttt 14280
cgaaatagaa cgttgccgac gacgccgaaa agccgaagcc ggtaagggcc gggccagcct 14340
tggcgacagc gaccgccgcg cacttcggaa gcatcatgcc gggcggaagg tcgataccgt 14400
gccacgcttc cagcaaggcc gccccgttgg tcgcgacggc gcttccggct tgcagcaaga 14460
cggcggcata ggtcgcattc ggtgcgccgt ctgtcgcaag ccctgcgacc gcagcgaagg 14520
ccgttttaat ccggtcgtcg attgtggcgc attgcgggtc gggcgcggta atcggcacgt 14580
cgtcgaagcc cacacaaggc accaacgccc ggaacgcgcc agacgatacg gcaagggcgt 14640
tcgcggtaag ctgcgtaatc gacagttctt cgccgacctt cgacagcgcg tcgataaact 14700
gcaacgtatg cgggcacgct accaagtctt cttcgatagg atgcgcgacg gtcaaaaccc 14760
cgtcggaagc cgccgcccaa ttgtgggcaa tatgcccgaa ctgaacgttc gtcggcccgg 14820
cttttttctg cgcgatggca acgaacttca acgccgccaa aagcgacgcc gcagggcttg 14880
gccccttcgc ctttttcttt ccggctgcgg cttgcctgcg cgcccttggc tttttcttcg 14940
gctgttcggc ggctggcgct tcgtcttcgg gcggaagcgg aatattactg tcgattttca 15000
ttttcggccc cttcttcgtc aagtttcgaa attgccgctt gaagatagtt cgctaagtct 15060
agcgtttctt ctagcgcgtg ttgcaaccaa tcgcgaagcg ataacgggtt attatccgtc 15120
gttacgccgt actttgcaag tccaacgatt gaacgttgca acagtaattc gcggttcttt 15180
tctacgtttt tatcgggcga tgtttcggcc atttgttcac cattcagcgg aaagaacttc 15240
gggatacttt ttgttcaccc atacgcgaac acggcgcggc gtgcgaagtt ctgaaacacg 15300
ctgcaaagct tgataagtcg tcggcggcgg ttcttcggcg tgccgctgcc gccaccaatc 15360
gcgcgaacgt ttcgcagcaa gccccggatg ttcaagcatt ataaattcgt tgaacatttg 15420
gaagccgcaa aagtaactaa ccttaatcat cggcggtttc gttagatttc cgtttgcgtc 15480
gcgcttttcg tgcaaattgt aaagtacctt ttgaacgtcg aaatattcga ctatcggcgc 15540
gtcgcttcgc agcggttcca gcgttccggc agttgcaaac aacttcgttt caaaagtaaa 15600
ttcggtgccg cagttgatac actgccgggc cgcagcgtga ttatatacgc cgcatacgtc 15660
gcaaatacga acgggcgcgt cgccgccgcc tttgcccggt ctgttcggaa ttcgcgggtc 15720
gttgattggg ccaagccttc gaacgtttcc ggcaaagtcc gcgtacaggc aattttgttt 15780
gccagtatcc ggcgacggtc gggttccgcg tcccaacttt tgaacgtgtt tgcccggcga 15840
tagcgtcggg ttcaaatcgc cgataaagtc gattggcgga tggtcgaaac ccgttgtaag 15900
tttctgccct gataccaagc cgcgaagttc gccagctttg aaagcgcgca agcgttcggt 15960
attggtcttc gaaggcaact tggaatgaac cggcaaaact tccaagccgt aagactgaat 16020
tacgttcgcg acgtgttcgg tattatcaat gccagttgcg aagataagcc aagtcgaacg 16080
gtcgtaagct atttcggtca tttcccgaac agcgttaaaa acaacttcgt cggtatctac 16140
ggcggcttct agttgcttgc tgttgaattc gccagccgtt acgcctactt tcgacaagtc 16200
gatttcggtt cgcgtcggcc ttgcgataag cggcgacaag tagccttcgg cgataagtcg 16260
gttaaacgat tcgacgccgg ttatatcgta acaaatgtcg gtaaaaattc cgttgtcggt 16320
aataagcccc attttcattc gatagggcgt tgccgtaaat ccaataactt taagatgcgg 16380
gttaatcttg cgaagttcgg cgataatgta ttggtaatgc gaatcttcct tgtcgcttaa 16440
caaatggcat tcgtcgatta acagcaagtc gcgccaaccg aaatggcgaa gatgcgccgg 16500
gcgtccgtcg tcttcttcaa gcccgcgttt aatcgcaggc gctacggact gaacgccgcc 16560
gaatacgatt ggcataatca tatcgcggga attcaagccc gccgaataaa tgcccattgg 16620
cgcggtcggc caaatcgaca taagcttttc ggcgttctgt tcgattagtt ctttaacgtg 16680
cgtaagcatc attattcgtt gattcggcca gtaaccgaaa attcgacgaa taaagtttgc 16740
aattacgacc gactttccgg ttcctgtcgg catggcgacg acggggtttc cgaccccgcc 16800
gcgctggaaa taatcgaata ttgaatattc cgcttcgtct tgataccaac gcggaacata 16860
aatattagac attctttgca accgctgtaa ttggcgaata agaatcgcaa cccgtagcga 16920
tgaaatcgcc cggaattacg ccgttgtgaa cttcgcaaaa ccattcgccg ttttcgacag 16980
gatttgcggc cttgcagctt cggcagttct tttcaggaat cgcgcccttg tggcaaatat 17040
ccttcgccgc gcaatatccg cacttgtgaa acgtcggatt atccgacaag cgcggcggcg 17100
cttcctgcga cagaatgatt tgttcggcct tctccttcat ttgctggcct agccgatggt 17160
ctagctttac aacttcgcaa tgcaagtcgt cgtcgttctt gttaatgttc aggtaaagaa 17220
cataacgaaa acgatattcg ttgccatacg tcgaagtttg ggcgaaatgc tgcggcttgc 17280
ttactgccat tccttgcgac attaagtccg aaaagccttt gccggttccg ttggtcttga 17340
attccagcaa aacgggttcg tcgataccgt agcgttcggg aagccgggcg atgccgtcaa 17400
gcgacccgcc gaagtgcccc ataacgccgg aaatgcggta ttgtgggaat tccaagccgt 17460
cggctttcgc gcgtgcgacg tgttgggcgt aatacggatt ttcgttcgta ataaggtcgc 17520
agcccaacgg cagttcttcg ccttccttca atatgccgta actgtcgctt tcggcttggt 17580
aaaagaaacc gtcgcggttt tcgtaccaac attgaacgcc gatgccttcc agccattcga 17640
taaagcgggc ttcttcacgg tgcccacgat taaacaagcg ttgcttgcgc ccgtcggttt 17700
gttcgcggaa acaccaacgg aagatatacc aaagcttccg cttgcattcg tcgccaatca 17760
aagacgcgcc aaggtgccga cggtgcccgc cgtcatacgt gcgaacgcaa tattcgtcta 17820
tatcttcttt aatgcgcttc gcaagggcgg ttgcaacgcc gggcgcgtca aggtctacgc 17880
tttgttttcc tgcgtcggat ttcgtcggct gcgcgtcgtt cagcgttaat tgtgcgttct 17940
tcgcgtcttt gctgttttgc atcgttcttc accttgcgcc gcataagggc ttcgtaattg 18000
ttcgctagat aatccgccga aagtgtcgca acgtctttaa gttggtcttt cgacagccaa 18060
gatatatggc attccgacgg gtcgattcct aattggttcg caagccaatt gtacgccttc 18120
gcgcgcgaca ttaagccggt ttgccaaagg cggtcgaatt cgttatgggc tttcgttcga 18180
agctggcgcg ttgttctgtc ggccatacga cccaacggaa taaacgtgcc cggatggcaa 18240
ccaacggcgg cgcgacaatc attgcaataa taaatgtgcg gccaattgcc ataagttcgc 18300
ccgtaaatct tgtcgttcgt tgttagttca atgttgaacg aacaacaagt atcgcattgt 18360
tcgggcgtcg gcaatgcgtc ttttattttc gacataagtt cgcatactcc ttaaaaagaa 18420
acgccggggc cggttaagac cccggcgaat ctacgccgcg acggcgttac gccttaacgc 18480
tggccccaag gtgccgcgcc tgccggtgcg ccgccctgcg cgggctggcc gccgccctgt 18540
tgccagccgc cgccctgcgg ttgctgctgc gcgggctgtt gggcaggctg gccgccccat 18600
gccggttggc cgccctgcgg ggccgggttc tgcgccggtt ggccgcccca agctgcgccg 18660
ccctgcggct gttgggccgg ttgctgcgcg ggctgctgct gtccgcccca agcggcaccg 18720
cctgcgggct gctgcggctg gccctgcggg gcaccgcccc aagcgccgtt cggctggccc 18780
tgcggctgct gttgctgcgg ctgcgctgcc gctgcgcctg cgccagcctt gcccggttcg 18840
ttcccgttca tatcgaagac ctttttaact tcggtatact gcgggtcgtt tttctgcggg 18900
ccgacttcga taagaaacgg caggttgtga agctgcgacg aatcggtaac ttggaacacg 18960
ccgattacgt ggcagattgc cgaaagctgg cgatgcgcga tttcgacggt ctgttggttg 19020
gaatggtaca ggttcaggcg ataagcgccg gtcgtgccct gctgcgggcc gtcgataatc 19080
cgcaagttca gttgcagata accgccgtcg ttcgccttgt tggccttgac ttcggaagat 19140
tcgacgataa ccggatgctt gccaatcggc aggcttccga cgccttgggt cgggtcgtac 19200
tgttgggcgt tgaacgcttg gataagttgc atgatgtttc accttttcga aaagtcttcg 19260
ccattggtta cggcggcgaa gtaaaccgtt ttattgcatc gctttcgcga acaatgcggt 19320
aaggtcgggc tgttcaagtt cgttaagatt gcccaaacgg tcgcgcgcga aaacttcggg 19380
aatttccttc gttcgcagcg cccgaaccgg cttcggcatt ccgggtacgc ttgcttcgcc 19440
caagtgcata acgttatcga acaaatgcgg aactttaacg ttaaggtctt tgcccggaaa 19500
gaacgggcgt ttttgcataa tcggttcgta agtaacttcg ccgttctgca aaatcgtttg 19560
ccgtccgttt tcgacaaggg cttgctttgc aatcattacg atatgctttt gcggcatgta 19620
atacaagtcg ttcgcgattt tcattacacg ttcggacata ttgccgtaag ccttcatacc 19680
gtgtttaacc ttgcccaatt cgtcggccaa gataatttcg gcgatgttcg aaatactgtc 19740
aatacccaac gtatcgaaat tcgcagcttc gcgcgatttc ataaaccatt cgaagaattc 19800
gacgataagc gcgggcgaat acgcttccca tgcgggaacg ttcgaaccgc gcatggacag 19860
cataccgggt tcggtcacaa gcaaaaccgg acgcggcgca gtattgataa gcggcgtttt 19920
gccggaaccg ggcgcaccga atacgacgga ctttacgccg tagcggcggg ccagttgcga 19980
cgccggtttt aattgcgaca tttgcataac ttgcaccttc gggaatggcc ccggcgcttg 20040
tgggcaaccg gggcggggtt gttacttcga cttcggcgcg acaagttcaa gcgcgggcgt 20100
tgcttccttc gacgttacaa cttcgtcgat aagcttacgg aacttgtcgg gaaggttctt 20160
gtattcggta acggaaagtt cgggcttcca ttttaccaaa cgttcggcga taaattcgcc 20220
ttcggggccg gtcttttcga ttttctgcaa agccttttcg acggcttctt ggccgcctgc 20280
gaagctgcgg ttaatcttaa aaacggcttt cagcttgtaa cctgcgccaa gttcgacgtt 20340
ttcggttcct tcgcgcaaag cttcggggtc gaaagcgaaa gcgtttttca gaacttcggc 20400
gcgcaatgcg gattcggctt ctttcgctgc ggcaagcgcc ttcgttgctt cctgccattg 20460
cagaatcaaa gcgtcgcggt tagcgttttc gttgaaagac attgttgtta ctccttcggt 20520
tgggccgccg cacaattgcg ccggtatgta cgaactatac gacggccctt cgttactgtc 20580
aagccccttt ttcaagaatt tttcgttctt cgaacaagtc gcgattgttg gcgtcgaatt 20640
cggtaaaggc gttcgggaag cgacgacgaa gcttcgcgat gttgaccgac tgcgcttcgc 20700
cgaagttcga accaatggcg cgcagcaaaa gcgcatcgta ccaaaagccg tcgccgactt 20760
cttcaagcgc gttaacagcg tcgaaagctt cgccttcggc tgcggcgctt agggcttcca 20820
acagttcgcc cgcttcggtc gccttgccga taattgcatg aatgacgttc acggctgcgg 20880
cgtcgtcttc ggggcggtcg gaaatccatt cgggaagctt cgccagcgtt gccgcgttgg 20940
cttccggggc cggaacgcca gcgtcgcgac cgtagaacat ggttttctcg attgcgtcaa 21000
gcttgttaag ggcttcgacg gcttcggcga tggtttcgcg gaagtaggcc agcgacacgc 21060
ggtcgccgta atacttatcc gacgcggtta cgtgcgcttc ttcgatatag tcgaaggtct 21120
ttgcgggttc gttcatacgt tcacctttta gaagggccgg gcaccatgcg ccagcgttcc 21180
agcactgtaa ccgaccgcgc ctttcctgtc aacaacaatt gacagcaagc aagaagccgg 21240
gtaagctgcc cacaattgct agaaggtggc gttatggaca ttgaagaact tacgaagcgg 21300
gtaaagaaag caagcgtcgg gcggtcgctg cgggcgctgg ccgcgcaatg caacgtttcg 21360
cacgaactta taagaaagct tttgcttgcc aattctgcgc cgaacataac ggcgacgact 21420
tataacaaga ttgataaagg attggcgaag aatggacttt aacaatattc cgcacgaaat 21480
gcggatttac ccgcaatggg tcgtttggcg atacgaagac acggattcaa agaaaccgac 21540
gaaggttccg tattcagcaa aaaccgggca ccttgccagc gtaaccgacc cgaacacttg 21600
ggccgggttc gacgaatgcg ttaacgctat gtcgtccggt tggtatgccg gaatcggttt 21660
cgtattgact gaaaacgacc cgtattcgtt tatcgacctt gacgacacaa aaggcgacca 21720
aacggcgctt gaccgccaaa taaagatttt taacgaattc aacagctacg ccgaacggtc 21780
gccttccggt tcggggttgc atatcatcgt taagggcgcg attccgtcgg gccgccgtcg 21840
ttcgtttatc gaagtttatt cttcgcttcg ttatatgaca atgaccggcg acgtttaccg 21900
caacgcaccg attaacgatt gtaacgaact gttaaacgtt ctttggggcc aaatggggca 21960
aggttcggtc gccgtcgcgc attatgccgg acttgccgaa gccaaggaaa ccgacgaaca 22020
ggtttataac cgcgcagtcg ccgcagccaa tggcgacaag ttcgccgaac tgtacgcggg 22080
caaatgggaa ggcatgtacg cttcgcagtc cgaagccgac tttgccttgg tcgatattat 22140
cgcgttttat acgcagaacc gggcgcagat ttcgcgaatg tttcgcgcgt cgggcttggg 22200
gcagcgtgac aaagcaaaac gcgacgatta cgtttcgtac atgctgaaca agtgtttcga 22260
ccgcatgttg ccgcccgttg acgttgacgg attgcgcaac aagctagacg aagccatagc 22320
gaagaaagaa gccgccgaca gggccgccgc gttgtcgcag aacagcgaag cgacgccgca 22380
tccgaaagcc ccggccccga accttagcga agtttcgaag gtatacagcg tgccgcccgg 22440
attagtcgga gaaattgcgc aatacattta cgcacaagcg ccgcgcccgg tgcccgaaat 22500
cgcgttggcc ggtgcccttg gcttggtcgc cggtattgtg ggcagggcgt acaacatttc 22560
cggcactggc cttaatcaat acgttctgtt gttggcaccg accggaacag gtaaagaagc 22620
catcgcaagc ggcattgata agctaatggc gcaggttatc cgaaccgttc ccgctgcgtc 22680
ggactttatc ggccccggtg aaattgcgtc ggcccaagcg attattaagt atatgtcgcg 22740
cggcccaacg tcgttcgttt cgttggtcgg cgaattcggc atttaccttc aacaaatggc 22800
aagcgtaaac gcaccgccgc accttaccgg gcttcgtcgc ttcctgttgg atgcttacaa 22860
caagtcgggc gaaggcaagg tacttcggcc ttcgatttat tcagacaagg acaagaacac 22920
gaccgccgtt ctttcgccgt cctttacctt gcttggcgaa tcgacgcccg aaaagtttta 22980
cgaaggtttg cacgaaggtt tgatttccga aggtctgttg ccgcgtttta cgatgattga 23040
atatcacggc gaacgcccgg cgttgaatcc ggggcacctg tcggcccaac cttcgttcga 23100
acttatcgac aggctttcga ccttgtgcgc ccatgcgcta atgctgaaca gccagcataa 23160
ggcgattcac gttcaaaccg acgcaaccgc ccgcgaactg ttccagcaat tcgacgcgca 23220
ttgcgacgcg aacattaaca caagcgaccg cgaagttcgg cggcaccttt ggaaccgggc 23280
gcacgtaaag gcgttgaagc tggccggaat tatcgccgtc ggctgcaacc cttacgaccc 23340
gaccattacc gccgacgttg cgtcttgggc gattaacctt gtcgtcgccg acgttcgaaa 23400
cttgcttgcc cggttcgatg ctggcgaaat tggcatagac aacgacgaaa cgaaacagct 23460
tgcgaaggtt attgcaaccg ttaaagattt cgtcgtttca ccttggccgg acgttgcgaa 23520
gtacgcaggc gaaggaatgt ccaaccttca ttcgaaccga attgttccgt acagctacgt 23580
tcaacgcagg cttgccgctg ttgccgtctt tcgtaaagac cgcatcggcg caagcggggc 23640
aatcaagcgc gccttgaaga ccctttgcga acgcggcgat ttgcaggaag taagccgggc 23700
cacactggcg aaggattacg gaacaagcgc cgttgcatac atggtcgcgc atccgggcgt 23760
tttcggcctg taacgcgcgc ccgccagtgt gggcagaacg ggccgccttc gggcggcttt 23820
ttcgtgcctg taaaaaaaag ttcgtcgtac ctgttgacgt agcttcgaac tgtacgtaga 23880
atacaaacca tcgaagcaac gaaacaaacc aaggaaccga agccatgaac gtaaccaacc 23940
ttaaaaccgc cgtcgaaatc aaaattgaaa ttgaattggc cgcttgggcc aacggcgaaa 24000
agaaagttaa cgtttccaac caattcgacg aaagcgtttt ggaatcggcc aaagaagcaa 24060
ttagcgtcga agcaatcgcc gcaatcgttg ccaagtatcc gggcgcgaac ttcgaaggct 24120
tttcggttat gacttgggcg cacgaacttt cggcgttcaa agccccggaa atcgaccggg 24180
cttggttcga ctaagcaagc gcccttcggg gcgttttttt tgttcgtacc tattgacgaa 24240
gcttcgaagt tgccgtatat ttaaaccatc gaagcaaaca acgaaggact aacgaacatg 24300
aacgcactta acaacattca acgggtcgta agctggaaca gcaagaagaa cgccgacggc 24360
acctttaccg ctaccgcgta cagcttcgac cataacgcgc cgaccgtcgt acatgccagc 24420
tacacgcgcg ccagccgcgc ccaagccgtc gcagacgcca agaaggccgt tcgttacctg 24480
aaagcccaac agcgcaccgc gtaaggagtc gccgccatgt ttgcccatta cttcgccagc 24540
aagaaaacga agttcgttgt aatcagcgaa acgaaccgcc cggaaggcca gcgaatcgcc 24600
gtaagcggca aagccgaagc ccgcagggtc gccaaggaac gcaacgcgca gccttggaac 24660
ttctaacccc ggccacggcc cttggcacgc gctaggggcc gcgcagcctg cccacattaa 24720
gcgaaggacg accgaccatg cgtacaaccc ttgcagccgt aaacaaagcc cttgccgccg 24780
ctggaatcga cgcggaattg gtcaagggtc gcgggtactt ttacttcgtt ggttccgccg 24840
tagattgcgc caaagaacaa ggcgtttacg gcgtgtatcg gctttccgaa ttgaccgttt 24900
cccaatgggt cgaagaagcc cgccaacgta tcgaaaagga gtaagaacaa atgaccgcat 24960
attttatcgc atttggcgtc gcgcttgggt tggcccttgc gcgcttggcc gcttcccaat 25020
tcgaagccgc ttgttacctg ttctgcaacg gcgaatttcg aaaggcattc cgggcaacga 25080
tgcacgccct tacctgcgtt gcgttgcttg cgttggttgt ttcgcccgtt gcttcgttct 25140
tcgatagctt cgaagttata agcattgaac aaccttcgcc caatccttcg aagggtcttc 25200
gcgtataata gccgacgggt cgtataatgg gcacgtataa gccttaaacc cttgatacat 25260
aaggctttca gcggattcat aacaagtata acgtataacg cccttgaccc tgaaaccccg 25320
gcccaatgtt ataaattaag ctttctttat atagattgat ttttagttat tatacttatt 25380
aaatttatta tatttcctta tttatcaagg acttagcagc tatacagccc cttcaattcg 25440
tataacgggc tttttgtgtt ataacgttgt tgacatacaa actttataag gctatgattc 25500
cggcacgcac taacgaaagg ggcttgcaat gtcttacgaa ataatcgaat gttcgccgaa 25560
ggcgcgaagc aaaattaagt cgttcgaaga tgcgctaggc gttgacgccg aattgacgca 25620
ccaacgttcg aagtatcctt ggccgctgtt gaaaatcggc cagtcgttcg ccgtgccgtt 25680
ggccgaaggt aacgaagcat cgttgcgaaa cggcgcgtcg cagttcagca agaagaccgg 25740
caagaagttt gcggttattc ggcattctga atacggctgt ttcgaatgcg cgcgcatcgc 25800
ttagcgccgg gccaatgtgg gcaacaaccg aaggggcaac caatgtacga aatcgaaacc 25860
ggccataaag tgccgaagca acgtcgcaag gctggaagcg tgccgaaggc cgttcaacag 25920
gcgttcaacg actacgccga agcgtataag gccgtttacg gcgttcgccc gctgtcgttc 25980
acgtatgacc gcgaaagcgg ctttattcgt gtcgaaagta gcggcggcgt aagcgtcgca 26040
aggctgcgcg aaatgacgaa acagcttcgt taccgaaaag gataaaaaaa ttcgttcgaa 26100
gggttgacgt actgtcgaag ccgtcgtact atatacccat accaacgaac acaaggaacc 26160
gccgaaatgc aaaacgtcca aatcaaacgc cccgcagcga ttaccgaagg ccaagcaatg 26220
aacgcagttt tcgacgcctt ccgccaagtg tggcgcaacg tcgaagcccg cgccgaaatg 26280
cttaaaggcg tttcgcagtc ggtcaaggtc ggcggcttcg aagtgtcggt tttcgcatgg 26340
cgcgacggtc gcattcaagc caacgtttac gcaggcggct ttaaccccgt cgcaaccgcc 26400
gaagttgacg aattcgacgc ctaatcaacc aagcgcgccg ggcgcaaccc ggcatttttg 26460
aaggagttat aacaatgtgc atcgtatgcg atattaaaca ggccgtcgaa gcttccggcc 26520
cgaacaaaga agaccgcgcc aaaatccttg gcttggtcga aaagctggcc gaagccttgg 26580
gcgatgttct ggaagtcgcg caggaagtac acgaagccaa gcccgaagcg ttccagccgg 26640
aacacgtcga acgcttgcag aacgccgccg acttgctgaa cgaccggggc gaactgcccg 26700
acgccctgtt ggccctgctg ttggccgccc ttgggcgcgg cgtcaaggtc gaaacaatcc 26760
gcgtcgaact gcgcgaaggc gaaacgccag aacaggccgt agcgcgtgta atggccgaac 26820
gcgaaggcgg ggccgcgacg aaacactaac caaagcgccc cggctgttgt atagtcgggg 26880
cttctttcta tcatggggaa cgaaagaaca tggcggacga agtagaccaa acagccgacc 26940
gaatagaaaa cgaagtaagc tttacggtcg gcgaactttg ccgaaaggcc gcagcgatac 27000
ccgcaggccg tccgggcgaa tgtgattttt gcggcgaaca cttcgcgcgc gttgtcgaag 27060
tgaccgaccc gcgaacgggc gaagtcgtcg aaagctgcgg tcgctgtcgt gaccgaaggg 27120
gcatcgcatg acgcccgcaa cccttgccaa gtctggcacc gaacacgcgc accaaacggc 27180
gctttttgcc tatgctgccg tcgcatactt acacggcttc gacgttgccg acgaatggtc 27240
aaagaccggc aagctaccga agcgcgaccc ggacgcaccg ccgaaggtgc ccgcccttga 27300
atggttccac gccataccga acggcggcag tcgcggcgac gacgacaagt cgcgaaagat 27360
acgcggcgcg cagttgaagg ccgaaggcgt gcggcagggc gtagccgata cgttcttgcc 27420
ttggcctgtt tacgaaccgt gcccaagcga cccgcaaata atgatgatga aatgggccgg 27480
tctttatatc gaaatgaaga agccgaccga acgtccgaag tcggaaaccg caaagggcgg 27540
cgtatcggac gaacaacgca gcttcggcga atacgcaaag cgcgtcggct atggttgggc 27600
cgtctgctat gattgggaac aagcggcgtc gtaccttcgt tcctatatcg aatggggttc 27660
gtaatggggc tttcgtgcct tgcctgcgaa cttacccgcg cccggttgaa agcggcggcg 27720
ctggcccttg tgggctggcc ccttgaccgc atcgccgggc atttgtcgtc atgctatggc 27780
gaacggtatt acgtcgaagg cggcaagctg tatcgcgcgt cgaagttgcc gccgtatcaa 27840
ccgcacttga taaggggcga agaatgaaaa agaccgacga agaattgata gacgaagccc 27900
gcagcattgc gcgttcgaaa cgctgtttcg tcgtaacgaa gccgggttat tggctgttat 27960
accgcgaatg cacgccgcgt aacgtatgcg tcggcaaacg gtcgtcggct gcggcgttgc 28020
ttcaacttgc gaaaaaggct tgacgtagct tcgaagctgt cgtacacttc ttcgtaagtt 28080
gaacgaacaa aggagtaacg accatgcccg catataacgt aacaaccgaa ttcgaaatga 28140
ccgacggtcg cgttctgtcg tgcgaatacg tcttgtcggt tacgcccggt cgctattccg 28200
gcccttggga agattgttac ccggacgaat ccgacgcagg cgaaccgacg tattttatcg 28260
acggcgaaga agtcgattac aaagaacttc caaaaggatt ggacgcaatc gccgacaagc 28320
tttacgaagc tggcgacggc gaatacaatt acaaggaaga acgcgacgaa ccggattacg 28380
aaccggatta cgattacgat tattaacgaa ggagtaacga aaatgtcttg gcttattggt 28440
tgggccgtac tttccgtcgg cgttgttgtc ggcttcttgc ttgcggcctt gctgcgggcc 28500
aacgggcgcg acgtgccggt tccttcggct accgttaccg ccctgtcgcc gcaggaacgc 28560
gcccgcatgg gctacgcgct ggcaagggct ggcgacttgc tggacagcgg cgaccttgaa 28620
tacgtgtcgg gcggctttat ggtgcaagac gtaggacacg gcgtcgtaat tatcaaaggc 28680
gatttgtcgt tgcgggttgc cgaagatgaa taaggacgaa gcgcgcgaaa tgttcgaagc 28740
cgtcaaggcc aaccgggcga agattgacgg ttgcgcccgc catcgcttcg aaatcggcga 28800
cccgccgtac acgttcgggc aaaagttcac ttgttcgaac tgcggcggaa caatggacgc 28860
cgtacaggcg ttccgatact gtcaaggctt caaggccgca ggcggcgacc cgaacgaagt 28920
tataccgggt tacgaataac gcttgacaag cggcgaaggt tcggcgaaac ttagacgtaa 28980
cgaagaaacc ccgttaagga gttgccgaaa tgaccgttct aattttgctt ctttgcaccg 29040
ttgccgcttt gttcattcat cgcatgtttg ttcgtccgat tctggattgg ttcgaacaac 29100
gcgaatacgc cgaacgtatg cgcgaagctg aacagcgcgc ggcgttggaa gaagcaaggc 29160
gggccaagtg tcgataatct acgcaccgcg cgacgccctg tcgaaatggg acgcgcgatt 29220
tattgaactg gccgaacacg tcgccggatg gtcgaaaggg ccgcgtaagc gcatcggcgc 29280
ggctatcgtg cgcccgaatc ggtcgattgc gtcgcttggg tacaacggcc cgccgcgcgg 29340
cttcgacgac gccgtttttc tgaacatgac gcgcgacgaa caacacgcgg tcgtaattca 29400
tgccgaagac aacgcaattc gacaggctgc gccgggcgaa gacttgtccg cgtgtacgct 29460
gtacgtttcg ccgctgtttc cgtgcgccat ttgtgccgac cgcattgttc gggccggtat 29520
ccgtcgcgtt gtcgcttatt gtgggcatat atcgccagac tggcgcgcgt ctgccgacga 29580
agccgaacgc atctttaacc aagcgggcgt cgaatgcctg tttacaatgg attaacgaac 29640
atgaaaccgc tagaccattc gcaaatcgaa ggcttgttgt tctatcaaga cccgacaacg 29700
caaaactata tcggcgcacc gaaggccgac ccgttcaacc aagccggaag cttcgtcgca 29760
ttcgggccgc gtgttaccgt tgacccggcg ttgtataact tgcttgccgc agcgccgaca 29820
atgtaccaag ccctttcgat gcaatacgct taccttcaag cgcaaatcga agttatcgaa 29880
cgcttgccaa aggcgggcga acttgataag ctgttgtcgt cgctaatcga agcgcagaac 29940
gcttgcctaa tggcccaacg tatcgcgcaa gtcggttacg aaaatgtcgc aaattcactt 30000
gacaaaggtt cgaagcggtc gtaagcttga accatcgaaa cgaacaaagg tgcccacaat 30060
gaaccgtaag cttcttgtta tcgctggaat cgtcgccttc gtcgcgttgc ttggcatggt 30120
cgggcaaatg gacttcgaag acgaagcccg cgcgcacctg ttgtattgtg acaacgtagc 30180
gaacggcgtt tggccggatt acgacaaaat ttacgattct gaatgcacgc ccgaacgcat 30240
cgcagaaatc gaagaaattt tgcgttgacc gcttgacacg aagaacggtc ggcgtaaaat 30300
caacccgtcg caacaacgcg acataacctt aacgttaagg agttacacaa aatggctacc 30360
aagaaatcga ccgcgaagaa gaacaccgcc gccccggtta tcggccttgc cgaaattgtt 30420
gcagccgggc cgaatggtct gtatacttcg cctgccgtac atggcccgct tgtcgaagcc 30480
ggtatggtgg aaatcaaccc ggcaatgacc aacgaagcgg gcgaaatcgc aacccgcgca 30540
acccaaaaag gaatcgacga aatgaaccag aacaccgaaa ccggcaccgc cgcagcttcc 30600
gccccggtcg ctacttcctt cgccatcgaa gacaacgtac ccatgccgac cggcagcggt 30660
cgcggtcgcg gcggcaacgt ctacccgttc gacgcgctgg aagtcggcca aagcttcttc 30720
gttccgaaca gcgaagaaaa gccgaacgcc gcgaagtcgc ttgcttcgac cgtttccagc 30780
gcaaccgccc gtttcgccgt gccttccgaa gacggcgcga ccaagaccaa caagaagggc 30840
gaaaccgtgc ccgtcatggt cgaaacccgc aagttcgttg ttcgttcggt cgaaggcggc 30900
gcgcgcgtct ggcgcaccaa gtaagcgacg gcgcgggcta cggcccgccc tacgcctacg 30960
cttcaacgga aaccccggcc attgcgccgg ggttttcttt tgcttttctt gtcaatttgt 31020
gctaatccgt gctaatcttc ggccaaatac atttcggggc cggggcttgt gatgaatgaa 31080
cgaacaacaa ttttttaacg tagccgtcgc aattatcggc gcgttgggcg gttggtggat 31140
gcgcgttatg tggcagtcac ttaaagaact tcaagcgcaa gattcgaagt tggccgataa 31200
agtcggcaat atcgaagttc ttgtagccgg gcagtatgtc aagcgcgacg atatgaaccg 31260
cgacataatg gcgattttcg ccaaacttga ccgcatcgaa gacaagatag acaaaaaggc 31320
cgacaaatga agaagcccga acttatcgaa gattggcgcg aatgctggcg cttttggtcg 31380
gttcgactgg ccgcagtcgg caccgcaatt actggccttc ttgttgcgtt tcccgacgca 31440
gcgttggccg cttgggcaat ccttccgcac gaattgaagt cgtcgattcc gccgcagtat 31500
atgccgctta tcggcgttgt tgttttcgcg ctttccatcg ttgcccgcct tgtcaagcaa 31560
aagaacttga acaaggggcc gaaggatgaa taacgaactt ccttgggtag ccgaagcccg 31620
aaagtatatc ggccttcgcg aagacacttc gaagacttcg cataacccga agcttcttgc 31680
catgcttgac cgcatgggcg aattttccaa cgaatcccgc gcttggtggc atgacgacga 31740
aacgccttgg tgcggtctgt tcgtcggcta ttgcttgggc gtagccgggc gctacgtcgt 31800
ccgcgaatgg tacagggcgc gggcatggga agccccgcag cttaccaagc ttgaccggcc 31860
cgcatacggc gcgcttgtga ccttcacgcg aagcggcggc ggccacgtcg gttttattgt 31920
gggcaaggat gcgcgcggta atcttatggt tcttggcggt aatcagtcga acgccgtaag 31980
tatcgcaccg ttcgcagtat cccgcgtaac cggctatttc tggccgtcgt tctggcgaaa 32040
caagaccgca gttaaaagcg ttccgttcga agaacgttat tcgctgccgc tgttgaagtc 32100
gaacggcgaa ctttcgacga atgaagctta aaggggcatc gcatgttttc cgtaatttgg 32160
gccaagtttg gcaaatggct tgccgcaatc ggcgcgggtc ttgtcatgct ggcaagtatt 32220
ttttatgccg gtcgcaagtc cggcgcaacc aaggccgaag ccaacgcagc aaacgaacgg 32280
gcaaacgacc gcgaagcaat cgcagttcgc caagttaacg aagcccgcga agcttccgaa 32340
caacaggtaa aggcggtaca aaatgcgaaa gacgttgctt ccgataattc tacttttgac 32400
gacgacgaag ttactaagcg gctgcgcgac gaatggtcgc gcgactgaac cgcaaagcat 32460
cgccgacgca gtgcaaacga agcccgttgt aatcgacacg gcttgcaaat gggttgcgcc 32520
tatttggatt tcgaaggccg acggcttgac cgctggcacg gcccggcaaa ttctgaacca 32580
taacgaagcc gtcgaacgca attgcgggcc gcagtcgccg cctaaatctg cggacaagcc 32640
ttaacagaac gcggggcttg cggtatagtg aagccaacga cgtataaact aggccgacac 32700
tatgtccgac gaactgaaag aaagcgaaga aaaggccgca tacgcggccc ttcttttgaa 32760
agaacgcgac ccgttcaagg ctgcgctttc gctgtttccg aacaacacga atcgcgcgct 32820
atgggttgcg aaccattggc cgaacgacgc cgaagttaag gccgaacaaa agcgccttac 32880
cgacgaaggc ggcgatatgg catttcttcc gggcaaaggc gaccttgccc gcgacatttg 32940
gcagcgtatg caaggtacga cgcttccgaa cggcgtaacc attccgccga cgcccgaaga 33000
atacgcgaag cttgccaagc tttacgccga cgttcgcggg ttcatcgaaa aaccgcaaac 33060
gaacgttaac gtaacgacga acgtaaaccg cgtcgtcgaa atgcccgtat tcgctaacga 33120
aagcgaatgg gaagcggaag ccgcacggca acagcgcgaa ttgttagaaa atgcacgcac 33180
tcgccattga tacgaacggg gcgaaacctg ttccgtatga agtcgtattt aagccccttc 33240
cggggtcgca gactatcgcc ctttgttcga tggcagcgca tacgctgtac gaaggcgcac 33300
gcggccccgg taagacgctt acgcagctta tgcgctttta tcgtaacgtc ggcaaaggct 33360
acggcaagtt ctggcgcggc gttatcttcg atttggaatt tgaccatttg ggcggccttg 33420
ttgccgaatc gaaaaaatgg ttcggcgata acggaaagct aaaagacggc ggcaagtttt 33480
acgaatcaac gtcggcttat aaatgggttt ggccgacagg cgaagaactg ttgtttcggc 33540
acgttaagaa gctgtcggat tacgaaggct tccacggtca cgaatacccg tttatcggtt 33600
ggaacgaact tacgaagcat ccaagcggcg acctttacga caaatttatg tcggtcaacc 33660
gctgtacgtt cgacccgata aaagacacgc cgaaagaccc gaaaaccggg cgctatttga 33720
cgccgaacgg cgaaccgctg ccgcctgtaa agtgcgaagt attcagcaca acgaacccaa 33780
gcggccccgg tcacaattgg gtaaaacgtc gctttattac gattgccccg cgcggcaccg 33840
tagttcgtcg cgaaattcag atttacaacc cggcgaccga aaaagaagaa acgcacgtaa 33900
ttagccaaat cgctatcttc ggttcgtata aggaaaaccc gtaccttccg gcttcgtata 33960
tcgccgaact ggaaagtatc aaagaaccga acttgcggaa ggcttggctt tacggcgatt 34020
gggacgttac cgcaggcggc gcaatcgacg acctttggca atcgcatata cacgttgtac 34080
cgcgcttcgt cattccgcca agctggcgaa tcgacagaac atacgacgac gggtcttcgc 34140
atccgtttag tgtgggctgg tgggcggaag cggacggcac cgaagcgacc atagttcttt 34200
cggatggcac cgaatttacg ttttgcccgc aaccgggttc gcttgttcag ttgttcgaat 34260
ggtacggatg cgcgaaagac gaaaaaggcg aatacatacc gaataaaggt ttgaagcttt 34320
cggcgtcgaa tatcgcacaa ggtataatcg accgcgaaat ttcgctaatg gcgaacggtt 34380
ggatttattc gcagccttgg cccggccctg ccgacaaccg cattcgccaa gtaatcgaca 34440
gcgaactaga cacgaccgaa aagcttatgt cgaagaaggg cgttcgttgg acggaatcgg 34500
acaaatcgcc gggttcgcgc gtaatcgggt tgcagctttt ccgcgaccgg ctagaagcgt 34560
ctgttaatcg cgaagggccg ggtatatact ttatgtcgaa ttgcgttgca agtattgatt 34620
tgctgcccac actgccgcgc gacgaaaaga agattgacga cgtagacacg accgccgaag 34680
accattgtta cgatatggtg cgttatcgcg tattgaaagg cgcgaacaaa gcggcagcga 34740
aattcaaact tgttatgcca acttaaagga acccaaatca tgccgaacgt ttcatttatt 34800
cgccccgaac ttggtaagct gctgccgctg tattacctga ttcgcgacgc aatcgcgggc 34860
gaaccaacgg taaaaggcgc gcggacgact tatcttccga tgccgaacgc cgaagaccaa 34920
tcgaaagaaa ataaggcgcg atacgaagcg tacttgaagc gggccgtttt ctataacgta 34980
gcccgtcgaa ccttgttcgg cttggtcggt caagtgttca tgcgcgaccc ggttgtaaag 35040
gttcccgcgc tgttgaaccc gcttgtcgca aacgcgacgg gttcgggtat caaccttacg 35100
cagcttgcga aaaaggccgt atcgctgaac cttgcgtatt cccgcgccgg gctgttggtc 35160
gattacccga cgaccgaagc cgaaggcggc gcgtcgattg ccgaccttga agccgggcgc 35220
attcgtccga ccctgtacgt atattcgccg accgaaatta tcaattggcg aacgaccgac 35280
cggggcgcgg aagaagtttt atcgttggtc gtactgttcg aaacttggtg cgctgccgat 35340
gatggttttg aaatgaaaac gtcggggcaa ttccgcgttt tgcgtttgga cgaagaaggt 35400
tattacgtcc acgaaatttg gcgcgaaccg cagccgacca aagccgacgg tacgaagatt 35460
ccgaaaggca attaccaaca atacacggtt tacaagccga cggacgcaaa cggcaatcgt 35520
cttaccgaaa ttccgtttat gttcatcggt tcggaaaaca acgattcgaa cccggacaac 35580
ccgaactttt acgaccttgc gtcgctgaat atggcgcatt atcgcaattc ggcggattac 35640
gaagaaagtt gttatatcgt cggacagccg acgccggttc ttatcggact taccgaagaa 35700
tgggttacga acgttcttaa aggttccgtt aacttcggtt cgcgcggcgg tattccgctt 35760
cctgccgggg ccgacgcgaa gttgcttcaa gcatccgaaa acaccatgct taaagaagca 35820
atggatacca aagaacgcca aatggtcgca cttggcgcaa agcttgtcga acaaaaggaa 35880
gtgcaacgca cggcaaccga agccgaattg gaagccgctt cggaaggttc gacgctttcc 35940
agtgcaacca agaacgtttc ggcggccttt gaatgggcgt tgaaatgggc ggcccgttgg 36000
gtcggtcaag ccgacagcgg cgttaagttc gaactgaata cagatttcga cattgcccgc 36060
atgacgccgg aagaacgccg ttcgcttgtt gaagaatggc agaaaggcgc aattaccttc 36120
gaagaaatgc gaaccggctt gcgtaaagct ggcgtcgcaa ccgaagacga cgccaaggct 36180
aaagaaaaaa tcgccaagga taccgccgaa gcaatggccc ttgctgcccc ggctaacgtg 36240
ccgggcgatg gcagcggcgg cgataatgtg ggcaacaacg gataaggggc gcaatcatgg 36300
cactatcgga caataagcga ctgtacgata ttgcgacgcg ccttgctgtt tacgtcgaag 36360
acgtgaaagt ttggcagtcc cgacaattcg ggttcgtact tcgcgaagta aacttggaat 36420
taacgcgcct tcttggtcgt gttcgttata agacccttga cggtctttcg aaggcgcagt 36480
taaacaagct tgtttcggaa ttacgggaat cgcaatcgaa gatttacagc gcatacacgc 36540
aaacgctgct tgaacagtta aaggaattca tgcgggccga cttggaagta aaccggcgcg 36600
cttgggttac gggttatatc gaacttgacg gcgaagaaag cgacgacatt atttcggacg 36660
aagaagcgat acagtttctt ttagaagtac caaacgccga ttcgaaccct ttgttcggtt 36720
tggccgctgt tacaggaagc gacgaacgca tttggtcgca agttacgaat acgccaattc 36780
cggcaaatgg tttgtacttg ctgccgttta tcaagacgtt tacgaattcg gcacaagctg 36840
gcgtcgaaag catgattcgc aaagcttggg cgaaccgttg gacggttgaa gaaaccttaa 36900
cggcgcttgt cggcgatgga accgcagcgc agggcacgcc gtcgcagttg caccgggtca 36960
acgcgcaagc cgcgtcggta atccatacgg caaccgcgca cgttgcggcg gtcgttgcgg 37020
ctggcgttat gtctgcggtt ttcggtcgtt acgtttggta ttcggtaatc gacggaagaa 37080
cgacggacat ttgcataagt cgaaatcgcc gaatctatcg cttcggcgaa gggccgttgc 37140
cgcccgcgca tatccgttgc cggtcgcacg ttgccccggc aaataccgct agtgacttgg 37200
ccgaagaaac gttttatact tgggttgccc gtcaacctgc ccaagtgcaa gacgacatat 37260
taggcgacga aggcggcgaa gctttgcgcg atggtcgctt aaaggcgaag gatattccga 37320
agtatgatgc ggacaggccg ctaacttacg aagaattccg acgcaagatt aaagaaattc 37380
tttcccgctg attcggtgaa tcggcataac cgcaaggagt ccttgaaatg gcactgaaaa 37440
agaaacttac caaagaagaa cacgcgaagc tttcggatgc actgaaagcc gaatatatcg 37500
aagacggcga cggcttccgc cttgacgttg acggcgacga agatacgggc gctttgaagc 37560
gcgccaaaga ccgcgaagcg cagttgcgca aagacgccga aaaggaagcg aaggaacttc 37620
gcgaacggct ggaatccatc gaaggcgacg acgcgcgcaa gaaaggcgac attgcgacgc 37680
ttgaaaagtc ttggcaatcg aagcttgaaa aacagcgcga agaatacgaa gccaaggttt 37740
ccaagcttac cgcgcatacg acgaaaacgc ttgtcgataa cgtcgcgtcg cagcttgccc 37800
acaagatttc gaacgccccg gcaatcatca tgccgcatat caagtcgcgg cttatcgcag 37860
acttcgaagg cgacacgccg gttacgcgcg ttctggacaa ggacggcaag ccgtcggccc 37920
ttactatcga cgaactggca aacgaattcg ttgcaaacaa ggatttttct gctattatca 37980
cagctagtaa ggcgtccggc ggtgccggta agccttcgca gaacggcggc ggtgccccga 38040
aatttaacgg tcaatccgac aaacccgccg acctttcgaa gatgaatccc gcagaacttg 38100
cggcgcatct taaagaagcg aaggcaaacg aaactaagga cgcttaatca tggcactttc 38160
cgaccttgct gtttactccg aatacgctta ttcggctttc tccgaaaccc tgcgccaaca 38220
ggttgatttg ttcaacgctg cgacgggcgg ggctatcatg ctgcaatccg cagcgcacca 38280
aggcgacttt tccgacgttg cctttttcgc caaggtaacg ggcggccttg ttcgccgtcg 38340
taacgcctac ggttccggca ccgtcgccga aaaggtcttg aaacaccttg tcgatacgtc 38400
ggttaaggtc gcagccggta cgccgcctgt tcgccttgac cccggccaat tccgttggat 38460
tcagcagaac ccggaagtcg caggcgcggc aatggggcag caactcgccg tcgatacgat 38520
ggcggatatg ctgaacgttg gtcttggttc ggtctattcg gcactttcgc aggttgcggc 38580
ggttgtttac gacgcgaccg gcaatactgc gcccgacgac ggcccgactt ggaacaacct 38640
taacaacggc caagccaagt tcggcgacca atccgcgcag attgcggcat ggattatgca 38700
cagtacgccc atgcacaagc tgtacggtaa caacctgaac aactccgaac gcctgtttac 38760
ttacggcacc gtgaacgttg ttcgcgaccc gttcggcaag cttcttgtta tgaccgattc 38820
gccaaacctg ttcgaagccg gtacgccgaa cgtttatcgc atccttggcc ttgtgccggg 38880
cgcggttatg attggtcaaa acaacgactt cgacgcgaac gaagaaacca agaacggcga 38940
cgaaaacatt attcggactt accaagccga atggtcgtac aacgtcggcg ttaagggctt 39000
cgcttgggac aaggcgaacg gcggcaagtc gccgaccgac gcggcgttgt tcacttcgac 39060
caattgggac aagtacgcaa cttccgaaaa ggatttggcg ggcgttgtcg tcgaagttca 39120
ctaacggacg gcagacggac ggggcttcgg ccccgttctt ctagtccttc acaaattcaa 39180
aggagtttcg aagatgaaac cggcaaaaat cctgtatttc gtcgatggca acgcaccgac 39240
cccggaagac ttcgcagccg ccgccgaact gaacgcgcaa gttatgttca ggaacgcccg 39300
cgctgttccg tccgaaccgc attcgctgga aatctgcgac ggcgtagcgg gcaaggttcc 39360
gaagctgtac gccgacgctt acccggaagc gggcgaagca atcaagaaga aggccgccga 39420
actgaaagcc cttgcttcga aggtcggcga cgccccggca ccgaaggcca gcggcaaggc 39480
cgccgacaag cccgcagccg ctacgcaggg ccagaaagcc ccggcagcgg ccccgcaggg 39540
cggcagcgcc ccggcttgga accctaaccc ggcgcagtaa ccaacggacg gcctgtcgct 39600
tattgtgggc aggccgtttc aacaacgaac taaggagtct tcgcaatggc aacgaaaaag 39660
gttgtttatt ttacggcagg cattaccgca accgctggcg aacttgccgc aattgcgaag 39720
cttaacgcag ccgccgaacc gcaatatgaa gttttggtag ccaatggcgc ggcaaatgcc 39780
gaatacggcg aaaccgaccg aatcattccg tcggattatg tcgccggtac tgttccttcg 39840
gaatattcgg cggttgacgt aatcgacccc gacgcaatcc cgaatcaggc gcttacgcca 39900
acgcaagcaa tcgttaacga cgccgaagcc ttgaccgttc cggttactgg cacttacacg 39960
acgaccgcaa ccgttagcgt tgcgaacggc gttgtaaccg gcatcgttct ttcgtaagga 40020
cgttgacgca tggcaattac aatcgttgtt gaagacggaa gcggcgtaac aaacgcgaac 40080
agttacgtaa gcgtcgcaga cgcgcgcatt tacgcttcga atcgcggcgt cgaacttccg 40140
ttggacgacg acgaattggc ggccatgctg attcgttcga cggattacct agaagcgcag 40200
gcttgccggt tccaaggcaa gccaacttcg acgacgcagg ctttgcaatg gccgcgaact 40260
ggcgtctttc ttaacgaaga cgaagtaccg tcgaacgtta ttccgaagtc gcttattgcc 40320
gcgcaagttc agcttgcaat ggcgattaac gcaggcttcg accttcaacc gaacgtttcg 40380
ccgcaagact acgttacgcg cgaaaaggtc gggccgattg aaacggaata cgccgacccg 40440
ctgtcggttg gcatcatgcc cacatttacc gcagcgaacg cacttcttgc gccgctgttt 40500
ggcgaatgcg cttcgaacaa gtttgcgctt cgtacaataa gggtttgacg aatggcacgt 40560
ttcgacgcag caattaaaac ggcgcaacgc cttatcgcaa agaacggcga aaaggtgaaa 40620
tggcgcgtta tcgaagacgc gacgccgaca gacccgaaca agccttggga acccggcccg 40680
gcattgccgg acgacaaaga cgttaccatt tgctttttgc cggttgaccg gcagacgcaa 40740
gaaactttca acttcattaa aggcaccgaa gttccgaaag gttcggtaat ggggcttatg 40800
ggcaatgttc cgtttgaacc gaatttgaaa gacgttgtaa ttcgtaacgg ggtcgaactt 40860
cgcttggctt atatcgacgt actttcgccg aacgggcaaa aggtacttta tacgatggta 40920
tttcaagcat gattgaattc gaccaagtta acgacgaagt aaacgcgctt tttcttgcaa 40980
cttggaacgc aggaagcgcg gcaattgcgg gttatgttcc cgaaattcgt tggcagggcg 41040
tgcaatatcg cgacttgccg gacggttcga agttttgggt tcggctgtcg aagcaaaccg 41100
tttttgaaga acaagcgacc ctttcaacct gcgaaggagt accggggcaa agaaaatata 41160
cggcgtcggg tcttgtcttc gtacaaatct tttgcccgaa atcgaatacg caagcgttcg 41220
aacttgggca gaaattggcg aaactttccc gtaatgcttt tcgcgggaaa tcgacgccgg 41280
gtaaggtttg gtttcgcaat acgcgaatta acgaacttcc gcccgaagaa ctttacgaac 41340
ggtttaacgt cgttaccgaa tttgaatacg acgaaatagg ttaaggagtt cttaacatgg 41400
tatgcgaaat tgccaaaatc gacagcaaca ttaccggact tgccttcgcc gaagaagaat 41460
gcttgaagca acttccgacg acgcccgttt ggtacggact ggaacccaac agctattcgg 41520
acttcggcgg cgaactttcg accgttgccc gcgcgcctat cgacccgtcg cgccagaaca 41580
agaaaggcac gattaccgac cttgacgcat cgggcggctt caacgccgac tttacgaaga 41640
ccaaccttac gcgaattctg caaggcttct tcttcgcaga cgcgcgcgaa cttccttcga 41700
cgcagccgct taacggcgca tcggtcgcac ttaccggcgt taccgcagtt gacagcactt 41760
acgccgcagc ttccgggctt ggcgtgttcg gtgccgatat gctggtatat gcgaccggct 41820
ttaccaacgc ggcgaacaac ggccttaaaa ccgtcgtttc ggctaccgct gcgggcgtcg 41880
ttgttgctga aaccttgatt gacgaaaccc cgcccgcagg cgcgaagctg gaatgcgtcg 41940
gtcgccagct tgccgccgca gacgcgaaca ttgccgtaac tggcaacgtc gtttcgctta 42000
tcgttaccgc tggcgacttt acgaccatgc ccgaactgtt ccccggtcgt tgggtcttcg 42060
tcggcggcga tgcgacggca aaccgcttcg ctaataatgt gggctatgcc cgcattaagt 42120
cggttgcagc gaaggcgctt gttttcgacg acgtgacttg gcaggcggcc aacgaaaccg 42180
gaaccggcaa gtcgattcgt cttttcgtcg gaactgttat caagaacgaa aagaccccgg 42240
cacttatcaa gcgtcgttcg tatcaaatcg aacgcacctt gggcgaaggt ttgaacggta 42300
cgcagtgcga atatctggaa ggcgcagtac cgaacgaatt tacgttgaac gttccgcagg 42360
ccgacaagct gaacgccgac ctttcgtttg ttgcgtgcga caacacttac cgcagcggcg 42420
accccggcga cgaacagaaa accggaaccc gcgtgcctgc gcccggcgaa gacgcttaca 42480
acacttcttc ggacgtttac cgaatcaaga tggccgttca cgacgccgcg tcgtcgaacc 42540
ccgccgccct gttcggctac gtttccgaag cgaacgtttc gattaacaac aacgttacgc 42600
cgaacaaggc ggtcggcgtg ttgggcgcgt tcgatacttc ggcgggtaac ttcgaagtcg 42660
gcggttcgat taccgcttac tttacgaccg tcgcagcggt taaggctgtt cgcgcgaacg 42720
ccgacgttgg tttgtcggta atcagtgcgg ccaagaacgc cggtttcgtg ttcgatattc 42780
cgttgcttgg tttgggcggc ggtcgtctga acgtcgaaaa agacgcgccg attaccgttc 42840
cgctggaacc cgcaggcgca gaaaacgcga acggctatac gatgctgtac gaagtgtttt 42900
cttatctgcc gaatctggca atgccggact aa 42932
<210> 17
<211> 783
<212> DNA
<213> Artificial Sequence
<220>
<223> RFP
<400> 17
ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca 60
caggaaacac atatggtttc taaaggtgaa gaagacaaca tggctatcat caaagaattt 120
atgcgtttca aagttcacat ggaaggttct gtgaacggtc acgaatttga aatcgaaggt 180
gaaggtgaag gtcgtccgta tgaaggcacc cagaccgcta aactgaaagt taccaaaggt 240
ggtccgctgc cgttcgcttg ggacatcctg tctccgcagt tcatgtacgg ttctaaagcg 300
tatgttaaac acccggctga catcccggac tacctgaaac tgtctttccc ggaaggtttc 360
aaatgggaac gtgttatgaa ctttgaagac ggtggtgttg ttaccgttac ccaggactct 420
tctctgcaag acggtgaatt tatctacaaa gttaaactgc gtggcaccaa cttcccgtct 480
gacggtccgg ttatgcagaa gaaaacgatg ggttgggaag cgtcttctga acgtatgtac 540
ccggaagacg gtgctctgaa aggtgaaatc aaacagcgtc tgaaactgaa agacggtggt 600
cactacgacg ctgaagttaa aaccacctac aaagctaaaa agccggttca actgccgggt 660
gcttacaacg tgaacatcaa actggacatc acctctcaca acgaagacta caccatcgtt 720
gaacagtacg aacgtgctga aggtcgtcac tctaccggcg gtatggacga actgtataaa 780
taa 783
<210> 18
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 18
cgcctgttta cttacggcac c 21
<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 19
cgcagatttc cagcgaatgc 20
<210> 20
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 20
gacgaccgac catgcgtaca a 21
<210> 21
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 21
tgggcgaagg ttgttcaatg c 21
<210> 22
<211> 11963
<212> DNA
<213> Artificial Sequence
<220>
<223> pYF-II
<400> 22
gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 60
cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 120
gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 180
aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 240
caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 300
ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcggtacc cggggatcct 360
ctagagtcga cctgcaggca tgcaagcttg agtattctat agtgtcacct aaatagcttg 420
gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 480
aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 540
acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 600
cattaatgaa tcggccaacg cgaacccctt gcggccgccc gggccgtcga ccaattctca 660
tgtttgacag cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc 720
gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg 780
ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca 840
aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata 900
tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa 960
actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt 1020
agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa 1080
ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg 1140
gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc 1200
catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata 1260
aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt 1320
ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca 1380
ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc 1440
tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg 1500
aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg 1560
cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac 1620
aggtatttat tcgcgataag ctcatggagc ggcgtaaccg tcgcacagga aggacagaga 1680
aagcgcggat ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg 1740
ccgccgctgc tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc 1800
attcctatgc gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga 1860
tgggacataa gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg 1920
ctgcccggca ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac 1980
aattatcctg agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg 2040
ctgtttttgt ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag 2100
tcgggaaaat ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc 2160
gtttatagga agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat 2220
atgccttcag gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg 2280
tcagcaatgg acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca 2340
gccagtagtg ctcgccgcag tcgagcgaca gggcgaagcc aagagtttgt agaaacgcaa 2400
aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt tatggcgggc 2460
gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat 2520
ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt 2580
tcgactgagc ctttcgtttt atttgatgcc tggttaggtg gcggtacttg ggtcgatatc 2640
aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc 2700
accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag 2760
gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc 2820
atagatatag atctcactac gcggctgctc aaacctgggc agaacgtaag ccgcgagagc 2880
gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa 2940
gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc 3000
acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca 3060
tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg 3120
cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca 3180
cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat 3240
aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg 3300
accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg 3360
tcaattcgct agagtcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg 3420
ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc 3480
cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc 3540
cctggccgac ggccagcagg taggccgaca ggctcatgcc ggctgccgcc gccttttcct 3600
caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg 3660
ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc 3720
agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga 3780
aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga 3840
tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg 3900
atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagc 3960
gctgcttccc tgctgttttg tggaatatct accgactgga aacaggcaaa tgcaggaaat 4020
tactgaactg aggggacagg cgagagacga tgccaaagag ctacaccgac gagctggccg 4080
agtgggttga atcccgcgcg gccaagaagc gccggcgtga tgaggctgcg gttgcgttcc 4140
tggcggtgag ggcggatgtc gaccgggagg gttcgagaag ggggggcacc ccccttcggc 4200
gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt 4260
ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa 4320
atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg 4380
tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc 4440
ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa 4500
ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc 4560
cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg 4620
tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc 4680
cggcggccgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg 4740
gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggctcgag tgagcgagga 4800
agcaccaggg aacagcactt atatattctg cttacacacg atgcctgaaa aaacttccct 4860
tggggttatc cacttatcca cggggatatt tttataatta ttttttttat agtttttaga 4920
tcttcttttt tagagcgcct tgtaggcctt tatccatgct ggttctagag aaggtgttgt 4980
gacaaattgc cctttcagtg tgacaaatca ccctcaaatg acagtcctgt ctgtgacaaa 5040
ttgcccttaa ccctgtgaca aattgccctc agaagaagct gttttttcac aaagttatcc 5100
ctgcttattg actctttttt atttagtgtg acaatctaaa aacttgtcac acttcacatg 5160
gatctgtcat ggcggaaaca gcggttatca atcacaagaa acgtaaaaat agcccgcgaa 5220
tcgtccagtc aaacgacctc actgaggcgg catatagtct ctcccgggat caaaaacgta 5280
tgctgtatct gttcgttgac cagatcagaa aatctgatgg caccctacag gaacatgacg 5340
gtatctgcga gatccatgtt gctaaatatg ctgaaatatt cggattgacc tctgcggaag 5400
ccagtaagga tatacggcag gcattgaaga gtttcgcggg gaaggaagtg gttttttatc 5460
gccctgaaga ggatgccggc gatgaaaaag gctatgaatc ttttccttgg tttatcaaac 5520
gtgcgcacag tccatccaga gggctttaca gtgtacatat caacccatat ctcattccct 5580
tctttatcgg gttacagaac cggtttacgc agtttcggct tagtgaaaca aaagaaatca 5640
ccaatccgta tgccatgcgt ttatacgaat ccctgtgtca gtatcgtaag ccggatggct 5700
caggcatcgt ctctctgaaa atcgactgga tcatagagcg ttaccagctg cctcaaagtt 5760
accagcgtat gcctgacttc cgccgccgct tcctgcaggt ctgtgttaat gagatcaaca 5820
gcagaactcc aatgcgcctc tcatacattg agaaaaagaa aggccgccag acgactcata 5880
tcgtattttc cttccgcgat atcacttcca tgacgacagg atagtctgag ggttatctgt 5940
cacagatttg agggtggttc gtcacatttg ttctgaccta ctgagggtaa tttgtcacag 6000
ttttgctgtt tccttcagcc tgcatggatt ttctcatact ttttgaactg taatttttaa 6060
ggaagccaaa tttgagggca gtttgtcaca gttgatttcc ttctctttcc cttcgtcatg 6120
tgacctgata tcgggggtta gttcgtcatc attgatgagg gttgattatc acagtttatt 6180
actctgaatt ggctatccgc gtgtgtacct ctacctggag tttttcccac ggtggatatt 6240
tcttcttgcg ctgagcgtaa gagctatctg acagaacagt tcttctttgc ttcctcgcca 6300
gttcgctatc acgtgctata aaaataatta taatttaaat tttttaatat aaatatataa 6360
attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat 6420
gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc ttcctgctct 6480
caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct 6540
gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg 6600
tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt 6660
tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa atccaaatac 6720
aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca ttaaaagata 6780
cgaggcgcgt gtaagttaca ggcaagcgat ccgtctaaga aaccattatt atcatgacat 6840
taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 6900
gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 6960
ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 7020
ttaactatgc ggcatcagag cagattgtac tgagagtgca ccataaattc ccgttttaag 7080
agcttggtga gcgctaggag tcactgccag gtatcgtttg aacacggcat tagtcaggga 7140
agtcataaca cagtcctttc ccgcaatttt ctttttctat tactcttggc ctcctctagt 7200
acactctata tttttttatg cctcggtaat gattttcatt tttttttttc cacctagcgg 7260
atgactcttt ttttttctta gcgattggca ttatcacata atgaattata cattatataa 7320
agtaatgtga tttcttcgaa gaatatacta aaaaatgagc aggcaagata aacgaaggca 7380
aagatgacag agcagaaagc cctagtaaag cgtattacaa atgaaaccaa gattcagatt 7440
gcgatctctt taaagggtgg tcccctagcg atagagcact cgatcttccc agaaaaagag 7500
gcagaagcag tagcagaaca ggccacacaa tcgcaagtga ttaacgtcca cacaggtata 7560
gggtttctgg accatatgat acatgctctg gccaagcatt ccggctggtc gctaatcgtt 7620
gagtgcattg gtgacttaca catagacgac catcacacca ctgaagactg cgggattgct 7680
ctcggtcaag catttaaaga ggccctaggg gccgtgcgtg gagtaaaaag gtttggatca 7740
ggatttgcgc ctttggatga ggcactttcc agagcggtgg tagatctttc gaacaggccg 7800
tacgcagttg tcgaacttgg tttgcaaagg gagaaagtag gagatctctc ttgcgagatg 7860
atcccgcatt ttcttgaaag ttttgcagag gctagcagaa ttaccctcca cgttgattgt 7920
ctgcgaggca agaatgatca tcaccgtagt gagagtgcgt tcaaggctct tgcggttgcc 7980
ataagagaag ccacctcgcc caatggtaca aacgatgttc cctccaccaa aggtgttctt 8040
atgtagcgct atgctcggtt acacggctgc ggcgagcgct agtgataata agtgactgag 8100
gtatgtgctc ttcttatctc cttttgtagt gttgctctta ttttaaacaa ctttgcggtt 8160
ttttgatgac tttgcgattt tgttgttgct ttgcagtaaa ttgcaagatt taataaaaaa 8220
acgcaaagca atgattaaag gatgttcaga atgaaactca tggaaacact taaccagtgc 8280
ataaacgctg gtcatgaaat gacgaaggct atcgccattg cacagtttaa tgatgacagc 8340
ccggaagcga ggaaaataac ccggcgctgg agaataggtg aagcagcgga tttagttggg 8400
gtttcttctc aggctatcag agatgccgag aaagcagggc gactaccgca cccggatatg 8460
gaaattcgag gacgggttga gcaacgtgtt ggttatacaa ttgaacaaat taatcatatg 8520
cgtgatgtgt ttggtacgcg attgcgacgt gctgaagacg tatttccacc ggtgatcggg 8580
gttgctgccc ataaaggtgg cgtttacaaa acctcagttt ctgttcatct tgctcaggat 8640
ctggctctga aggggctacg tgttttgctc gtggaaggta acgaccccca gggaacagcc 8700
tcaatgtatc acggatgggt accagatctt catattcatg cagaagacac tctcctgcct 8760
ttctatcttg gggaaaagga cgatgtcact tatgcaataa agcccacttg ctggccgggg 8820
cttgacatta ttccttcctg tctggctctg caccgtattg aaactgagtt aatgggcaaa 8880
tttgatgaag gtaaactgcc caccgatcca cacctgatgc tccgactggc cattgaaact 8940
gttgctcatg actatgatgt catagttatt gacagcgcgc ctaacctggg tatcggcacg 9000
attaatgtcg tatgtgctgc tgatgtgctg attgttccca cgcctgctga gttgtttgac 9060
tacacctccg cactgcagtt tttcgatatg cttcgtgatc tgctcaagaa cgttgatctt 9120
aaagggttcg agcctgatgt acgtattttg cttaccaaat acagcaatag taatggctct 9180
cagtccccgt ggatggagga gcaaattcgg gatgcctggg gaagcatggt tctaaaaaat 9240
gttgtacgtg aaacggatga agttggtaaa ggtcagatcc ggatgagaac tgtttttgaa 9300
caggccattg atcaacgctc ttcaactggt gcctggagaa atgctctttc tatttgggaa 9360
cctgtctgca atgaaatttt cgatcgtctg attaaaccac gctgggagat tagataatga 9420
agcgtgcgcc tgttattcca aaacatacgc tcaatactca accggttgaa gatacttcgt 9480
tatcgacacc agctgccccg atggtggatt cgttaattgc gcgcgtagga gtaatggctc 9540
gcggtaatgc cattactttg cctgtatgtg gtcgggatgt gaagtttact cttgaagtgc 9600
tccggggtga tagtgttgag aagacctctc gggtatggtc aggtaatgaa cgtgaccagg 9660
agctgcttac tgaggacgca ctggatgatc tcatcccttc ttttctactg actggtcaac 9720
agacaccggc gttcggtcga agagtatctg gtgtcataga aattgccgat gggagtcgcc 9780
gtcgtaaagc tgctgcactt accgaaagtg attatcgtgt tctggttggc gagctggatg 9840
atgagcagat ggctgcatta tccagattgg gtaacgatta tcgcccaaca agtgcttatg 9900
aacgtggtca gcgttatgca agccgattgc agaatgaatt tgctggaaat atttctgcgc 9960
tggctgatgc ggaaaatatt tcacgtaaga ttattacccg ctgtatcaac accgccaaat 10020
tgcctaaatc agttgttgct cttttttctc accccggtga actatctgcc cggtcaggtg 10080
atgcacttca aaaagccttt acagataaag aggaattact taagcagcag gcatctaacc 10140
ttcatgagca gaaaaaagct ggggtgatat ttgaagctga agaagttatc actcttttaa 10200
cttctgtgct taaaacgtca tctgcatcaa gaactagttt aagctcacga catcagtttg 10260
ctcctggagc gacagtattg tataagggcg ataaaatggt gcttaacctg gacagatctc 10320
gtgttccaac tgagtgtata gagaaaattg aggccattct taaggaactt gaaaagccag 10380
caccctgatg cgaccacgtt ttagtctacg tttatctgtc tttacttaat gtcctttgtt 10440
acaggccaga aagcataact ggcctgaata ttctctctgg gcccactgtt ccacttgtat 10500
cgtcggtctg ataatcagac tgggaccacg gtcccactcg tatcgtcggt ctgattatta 10560
gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc 10620
cactcgtatc gtcggtctga taatcagact gggaccacgg tcccactcgt atcgtcggtc 10680
tgattattag tctgggacca tggtcccact cgtatcgtcg gtctgattat tagtctggga 10740
ccacggtccc actcgtatcg tcggtctgat tattagtctg gaaccacggt cccactcgta 10800
tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt 10860
agtctgggac cacgatccca ctcgtgttgt cggtctgatt atcggtctgg gaccacggtc 10920
ccacttgtat tgtcgatcag actatcagcg tgagactacg attccatcaa tgcctgtcaa 10980
gggcaagtat tgacatgtcg tcgtaacctg tagaacggag taacctcggt gtgcggttgt 11040
atgcctgctg tggattgctg ctgtgtcctg cttatccaca acattttgcg cacggttatg 11100
tggacaaaat acctggttac ccaggccgtg ccggcacgtt aacttggtaa cgaatcagac 11160
aattgacggc ttgacggagt agcatagggt ttgcagaatc cctgcttcgt ccatttgaca 11220
ggcccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 11280
gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg 11340
ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc 11400
aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttcggg 11460
ctgcatccga tgcaagtgtg tcgctgtcga cgagctcgcg agctcggaca tgaggttgcc 11520
ccgtattcag tgtcgctgat ttgtattgtc tgaagttgtt tttacgttaa gttgatgcag 11580
atcaattaat acgatacctg cgtcataatt gattatttga cgtggtttga tggcctccac 11640
gcacgttgtg atatgtagat gataatcatt atcactttac gggtcctttc cggtgatccg 11700
acaggttacg gggcggcgac ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa 11760
ggcgtttccg ttcttcttcg tcataactta atgtttttat ttaaaatacc ctctgaaaag 11820
aaaggaaacg acaggtgctg aaagcgagct ttttggcctc tgtcgtttcc tttctctgtt 11880
tttgtccgtg gaatgaacaa tggaagtccg agctcatcgc taataacttc gtatagcata 11940
cattatacga agttatattc gat 11963
Claims (13)
1.一种多核苷酸，包含第一片段和与所述第一片段的3’端连接第二片段，其中所述第一片段的核苷酸序列是环状重排的Headful包装型噬菌体的单位长度的基因组序列，且其中所述第二片段的核苷酸序列是所述第一片段核苷酸序列的核苷酸1到n，n为至少40。
2.权利要求1的多核苷酸，其中n为至少60。
3.权利要求1或2的多核苷酸，其中所述单位长度的基因组是野生型基因组。
4.权利要求1-3任一项的多核苷酸，其中所述第一片段的核苷酸序列由SEQ ID NO:13-16之一的核苷酸序列组成。
5.权利要求1或2的多核苷酸，其中所述单位长度的基因组是经修饰的基因组，其中与野生型基因组相比，所述经修饰的基因组包含一或多个核苷酸的插入、缺失、取代或添加。
6.权利要求5的多核苷酸，其中所述第一片段的核苷酸序列是SEQ ID NO:13-16之一的核苷酸序列的经修饰的变体。
7.一种载体，包含权利要求1-6任一项的多核苷酸。
8.一种细胞，如酵母细胞，包含权利要求1-6任一项的多核苷酸，或权利要求7的载体。
9.一种合成Headful包装型噬菌体的方法，包括用权利要求1-6任一项的多核苷酸，或权利要求7的载体转化的细菌的步骤。
10.权利要求9的方法，其中所述细菌是所述噬菌体的天然宿主。
11.权利要求9或10的方法，还包括在酵母中基于转化相关的重组(TAR)组装所述载体的步骤。
12.权利要求11的方法，其中所述酵母是酿酒酵母(Saccharomyces cerevisiae)。
13.权利要求9-12任一项的方法，其中所述载体是酵母-细菌穿梭载体。
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