CN113261663A - Production process of enzyme for food - Google Patents
Production process of enzyme for food Download PDFInfo
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- CN113261663A CN113261663A CN202110640241.2A CN202110640241A CN113261663A CN 113261663 A CN113261663 A CN 113261663A CN 202110640241 A CN202110640241 A CN 202110640241A CN 113261663 A CN113261663 A CN 113261663A
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- ultrafiltration
- microcrystalline cellulose
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 55
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 55
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 235000013305 food Nutrition 0.000 title claims abstract description 17
- 229940088598 enzyme Drugs 0.000 claims abstract description 53
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 51
- 238000000855 fermentation Methods 0.000 claims abstract description 35
- 230000004151 fermentation Effects 0.000 claims abstract description 35
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 33
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 25
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 25
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims abstract description 25
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims abstract description 25
- 239000008108 microcrystalline cellulose Substances 0.000 claims abstract description 25
- 229940016286 microcrystalline cellulose Drugs 0.000 claims abstract description 25
- 238000001556 precipitation Methods 0.000 claims abstract description 25
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 22
- 238000003825 pressing Methods 0.000 claims abstract description 19
- 108010015776 Glucose oxidase Proteins 0.000 claims abstract description 17
- 239000004366 Glucose oxidase Substances 0.000 claims abstract description 17
- 229940116332 glucose oxidase Drugs 0.000 claims abstract description 17
- 235000019420 glucose oxidase Nutrition 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 235000017060 Arachis glabrata Nutrition 0.000 claims abstract description 11
- 244000105624 Arachis hypogaea Species 0.000 claims abstract description 11
- 235000010777 Arachis hypogaea Nutrition 0.000 claims abstract description 11
- 235000018262 Arachis monticola Nutrition 0.000 claims abstract description 11
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 11
- 240000008042 Zea mays Species 0.000 claims abstract description 11
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 11
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 11
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 11
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 11
- 235000005822 corn Nutrition 0.000 claims abstract description 11
- 235000013312 flour Nutrition 0.000 claims abstract description 11
- 235000021552 granulated sugar Nutrition 0.000 claims abstract description 11
- 235000012054 meals Nutrition 0.000 claims abstract description 11
- 235000020232 peanut Nutrition 0.000 claims abstract description 11
- 239000004599 antimicrobial Substances 0.000 claims abstract description 6
- 239000008213 purified water Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 20
- 229960004106 citric acid Drugs 0.000 claims description 15
- 235000015165 citric acid Nutrition 0.000 claims description 15
- 239000002244 precipitate Substances 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 15
- 235000010216 calcium carbonate Nutrition 0.000 claims description 10
- 230000001376 precipitating effect Effects 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 239000003242 anti bacterial agent Substances 0.000 claims description 5
- 239000011876 fused mixture Substances 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 238000001035 drying Methods 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 description 9
- 244000052769 pathogen Species 0.000 description 8
- 239000010802 sludge Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 239000006260 foam Substances 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 239000002674 ointment Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000013527 bean curd Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011942 biocatalyst Substances 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
- A23L29/262—Cellulose; Derivatives thereof, e.g. ethers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of production of enzymes for food, and particularly relates to a production process of the enzymes for food, which comprises 10-30 parts of edible corn flour, 40-50 parts of peanut meal, 5-7 parts of white granulated sugar, 6-7 parts of aspergillus niger, 3-5 parts of calcium carbonate, 5-8 parts of bacillus subtilis, 5-9 parts of glucose oxidase, 10-20 parts of yeast, 6-10 parts of an anticaking agent, 2-3 parts of an anti-microbial agent, 3-10 parts of citric acid, 9-13 parts of microcrystalline cellulose and 10-30 parts of purified water. According to the invention, the fermentation is carried out after the crushing, the filter pressing and the ultrafiltration are carried out after the fermentation, the precipitation is carried out after the ultrafiltration, the drying is carried out after the precipitation, the operation is simple and convenient, the cost is low, and in the precipitation, the bacillus subtilis, the glucose oxidase, the citric acid and the microcrystalline cellulose are added into the concentrated fermentation liquor, so that the enzymolysis effect of the obtained edible enzyme is better.
Description
Technical Field
The invention belongs to the technical field of production of food enzymes, and particularly relates to a production process of a food enzyme.
Background
The enzyme preparation is used as a high-efficiency biocatalyst and has been widely applied to the industries of food, leather and feed. The enzyme liquid produced by liquid fermentation is made into dried bean curd, which is convenient for storage, transportation and processing. The spray drying has the advantages of low material cost, no operation processes of evaporation, crystallization, separation, crushing and the like, continuous automatic production and stable operation.
The enzyme comes from the nature, and is a natural catalyst for thousands of biochemical reactions which are rapidly and efficiently carried out in organisms at any moment. The natural sources of enzymes are animal, plant and microorganism. The enzyme preparations currently used for large-scale industrial production are produced by microbial fermentation. The food enzyme preparation acts only during the processing process after being added to food as a food additive, i.e. helps a substance to complete a transformation, and once it has completed its mission, it goes back and disappears or loses its vitality in the final product. No harmful residue is generated in the food. The production process of the existing food enzyme production process is complex, and other bacteria or pathogenic bacteria can be generated in the production process.
Disclosure of Invention
To solve the problems set forth in the background art described above. The invention provides a production process of food enzyme, which has the characteristics of simple production process and better enzymolysis effect of the obtained edible enzyme by adding bacillus subtilis, glucose oxidase, citric acid and microcrystalline cellulose into concentrated fermentation liquor.
In order to achieve the purpose, the invention provides the following technical scheme: a production process of food enzyme comprises 10-30 parts of edible corn flour, 40-50 parts of peanut meal, 5-7 parts of white granulated sugar, 6-7 parts of Aspergillus niger, 3-5 parts of calcium carbonate, 5-8 parts of Bacillus subtilis, 5-9 parts of glucose oxidase, 10-20 parts of yeast, 6-10 parts of an anticaking agent, 2-3 parts of an antibacterial agent, 3-10 parts of citric acid, 9-13 parts of microcrystalline cellulose and 10-30 parts of purified water, and the production process comprises the following steps:
the method comprises the following steps: crushing and mixing edible corn flour and peanut meal, putting the crushed and fused mixture into a fermentation container, adding pure water, wherein the ratio of the pure water to the mixture is 1:2 or 2:3, then continuously adding white granulated sugar, aspergillus niger, yeast, an anti-caking agent and an anti-microbial agent into the fermentation container, and after stirring, fermenting for 5-7 days at 30-60 ℃ to obtain fermentation liquor;
step two: taking out the fermentation liquor, performing filter pressing, and performing ultrafiltration concentration after filter pressing;
step three: concentrating, and precipitating with solvent;
step four: the precipitate obtained by precipitation was dried.
Preferably, the second step is filter-pressed by a plate frame, and the ultrafiltration concentration is concentrated by an ultrafiltration membrane
Preferably, calcium carbonate, bacillus subtilis, glucose oxidase, citric acid and microcrystalline cellulose are added into the ultrafiltration concentrated solution obtained in the three-way step two, and stirring and mixing are carried out.
Preferably, the stirred mixed solution obtained in the fourth step is precipitated, the precipitated precipitate is enzyme mud, and the enzyme mud is air-dried or aired.
Preferably, the temperature range for the precipitation in step three is 8-11 ℃.
Preferably, the dried precipitate is obtained in the fourth step, and vacuum bagging is carried out after briquetting and cutting.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the fermentation is carried out after the crushing, the filter pressing and the ultrafiltration are carried out after the fermentation, the precipitation is carried out after the ultrafiltration, the drying is carried out after the precipitation, the operation is simple and convenient, the cost is low, and in the precipitation, the bacillus subtilis, the glucose oxidase, the citric acid and the microcrystalline cellulose are added into the concentrated fermentation liquor, so that the enzymolysis effect of the obtained edible enzyme is better.
2. According to the invention, through the addition of the bacillus subtilis, the bacillus subtilis generates antibiotic substances capable of inhibiting the growth of bacteria, viruses, fungi and pathogens, so that the bacteria and the pathogens can be generated in the process of producing the edible enzyme, the edible enzyme is safer and healthier, and through the addition of microcrystalline cellulose, the microcrystalline cellulose can stabilize foam and emulsion in the processing process, form gel with ointment-like texture, and facilitate the forming of the edible enzyme.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The invention provides the following technical scheme: the invention provides the following technical scheme: a production process of food enzyme comprises 10 parts of edible corn flour, 40 parts of peanut meal, 5 parts of white granulated sugar, 6 parts of Aspergillus niger, 3 parts of calcium carbonate, 5 parts of Bacillus subtilis, 6 parts of glucose oxidase, 12 parts of yeast, 6 parts of an anticaking agent, 2 parts of an antibacterial agent, 3 parts of citric acid, 9 parts of microcrystalline cellulose and 10 parts of purified water, and the production process comprises the following steps:
the method comprises the following steps: crushing and mixing edible corn flour and peanut meal, putting the crushed and fused mixture into a fermentation container, adding pure water, wherein the ratio of the pure water to the mixture is 1:2, then continuously adding white granulated sugar, aspergillus niger, yeast, an anti-caking agent and an anti-microbial agent into the fermentation container, and after stirring, fermenting for 6 days at 39 ℃ to obtain fermentation liquor;
step two: taking out the fermentation liquor, performing filter pressing, and performing ultrafiltration concentration after filter pressing;
step three: concentrating, and precipitating with solvent;
step four: the precipitate obtained by precipitation was dried.
The edible enzyme is prepared by crushing, fermenting, performing filter pressing and ultrafiltration after fermentation, performing precipitation after ultrafiltration, drying after precipitation, and has the advantages of simple and convenient operation and low cost, and during precipitation, bacillus subtilis, glucose oxidase, citric acid and microcrystalline cellulose are added into concentrated fermentation liquor to ensure that the obtained edible enzyme has better enzymolysis effect.
Specifically, the second step is filter-pressing by a plate frame, and the ultrafiltration concentration is carried out by an ultrafiltration membrane.
Specifically, calcium carbonate, bacillus subtilis, glucose oxidase, citric acid and microcrystalline cellulose are added into the ultrafiltration concentrated solution obtained in the three-way step II, the ultrafiltration concentrated solution is stirred and mixed, the bacillus subtilis generates antibiotic substances capable of inhibiting the growth of bacteria, viruses, fungi and pathogens by adding the bacillus subtilis, the bacteria and the pathogens can be generated in the process of producing the edible enzyme, the edible enzyme is safer and healthier, and the microcrystalline cellulose can stabilize foam and emulsion in the processing process by adding the microcrystalline cellulose to form gel similar to ointment texture, so that the edible enzyme is convenient to form.
And specifically, precipitating the stirred mixed solution obtained in the fourth step, wherein the precipitated precipitate is enzyme sludge, and the enzyme sludge is air-dried or aired.
Specifically, the temperature at the time of precipitation in the third step was 11 ℃.
Specifically, the dried precipitate is obtained in the fourth step, and is pressed into blocks and cut into pieces for vacuum bagging.
Example 2
The invention provides the following technical scheme: a production process of food enzyme comprises 15 parts of edible corn flour, 45 parts of peanut meal, 6 parts of white granulated sugar, 6 parts of Aspergillus niger, 4 parts of calcium carbonate, 6 parts of Bacillus subtilis, 7 parts of glucose oxidase, 15 parts of yeast, 9 parts of an anticaking agent, 3 parts of an antibacterial agent, 10 parts of citric acid, 12 parts of microcrystalline cellulose and 18 parts of purified water, and the production process comprises the following steps:
the method comprises the following steps: crushing and mixing edible corn flour and peanut meal, putting the crushed and fused mixture into a fermentation container, adding pure water, wherein the ratio of the pure water to the mixture is 2:3, then continuously adding white granulated sugar, aspergillus niger, yeast, an anti-caking agent and an anti-microbial agent into the fermentation container, and after stirring, fermenting for 5 days at 43 ℃ to obtain fermentation liquor;
step two: taking out the fermentation liquor, performing filter pressing, and performing ultrafiltration concentration after filter pressing;
step three: concentrating, and precipitating with solvent;
step four: the precipitate obtained by precipitation was dried.
The edible enzyme is prepared by crushing, fermenting, performing filter pressing and ultrafiltration after fermentation, performing precipitation after ultrafiltration, drying after precipitation, and has the advantages of simple and convenient operation and low cost, and during precipitation, bacillus subtilis, glucose oxidase, citric acid and microcrystalline cellulose are added into concentrated fermentation liquor to ensure that the obtained edible enzyme has better enzymolysis effect.
Specifically, the second step is filter-pressing by a plate frame, and the ultrafiltration concentration is carried out by an ultrafiltration membrane.
Specifically, calcium carbonate, bacillus subtilis, glucose oxidase, citric acid and microcrystalline cellulose are added into the ultrafiltration concentrated solution obtained in the three-way step II, the ultrafiltration concentrated solution is stirred and mixed, the bacillus subtilis generates antibiotic substances capable of inhibiting the growth of bacteria, viruses, fungi and pathogens by adding the bacillus subtilis, the bacteria and the pathogens can be generated in the process of producing the edible enzyme, the edible enzyme is safer and healthier, and the microcrystalline cellulose can stabilize foam and emulsion in the processing process by adding the microcrystalline cellulose to form gel similar to ointment texture, so that the edible enzyme is convenient to form.
And specifically, precipitating the stirred mixed solution obtained in the fourth step, wherein the precipitated precipitate is enzyme sludge, and the enzyme sludge is air-dried or aired.
Specifically, the temperature at the time of precipitation in the third step was 10 ℃.
Specifically, the dried precipitate is obtained in the fourth step, and is pressed into blocks and cut into pieces for vacuum bagging.
Example 3
The invention provides the following technical scheme: a production process of food enzyme comprises 30 parts of edible corn flour, 50 parts of peanut meal, 7 parts of white granulated sugar, 7 parts of Aspergillus niger, 5 parts of calcium carbonate, 7 parts of Bacillus subtilis, 8 parts of glucose oxidase, 19 parts of yeast, 9 parts of an anticaking agent, 3 parts of an antibacterial agent, 9 parts of citric acid, 13 parts of microcrystalline cellulose and 30 parts of purified water, and the production process comprises the following steps:
the method comprises the following steps: crushing and mixing edible corn flour and peanut meal, putting the crushed and fused mixture into a fermentation container, adding pure water, wherein the ratio of the pure water to the mixture is 2:3, then continuously adding white granulated sugar, aspergillus niger, yeast, an anti-caking agent and an anti-microbial agent into the fermentation container, and after stirring, fermenting for 5 days at 60 ℃ to obtain fermentation liquor;
step two: taking out the fermentation liquor, performing filter pressing, and performing ultrafiltration concentration after filter pressing;
step three: concentrating, and precipitating with solvent;
step four: the precipitate obtained by precipitation was dried.
The edible enzyme is prepared by crushing, fermenting, performing filter pressing and ultrafiltration after fermentation, performing precipitation after ultrafiltration, drying after precipitation, and has the advantages of simple and convenient operation and low cost, and during precipitation, bacillus subtilis, glucose oxidase, citric acid and microcrystalline cellulose are added into concentrated fermentation liquor to ensure that the obtained edible enzyme has better enzymolysis effect.
Specifically, the second step is filter-pressing by a plate frame, and the ultrafiltration concentration is carried out by an ultrafiltration membrane.
Specifically, calcium carbonate, bacillus subtilis, glucose oxidase, citric acid and microcrystalline cellulose are added into the ultrafiltration concentrated solution obtained in the three-way step II, the ultrafiltration concentrated solution is stirred and mixed, the bacillus subtilis generates antibiotic substances capable of inhibiting the growth of bacteria, viruses, fungi and pathogens by adding the bacillus subtilis, the bacteria and the pathogens can be generated in the process of producing the edible enzyme, the edible enzyme is safer and healthier, and the microcrystalline cellulose can stabilize foam and emulsion in the processing process by adding the microcrystalline cellulose to form gel similar to ointment texture, so that the edible enzyme is convenient to form.
And specifically, precipitating the stirred mixed solution obtained in the fourth step, wherein the precipitated precipitate is enzyme sludge, and the enzyme sludge is air-dried or aired.
Specifically, the temperature at the time of precipitation in the third step was 10 ℃.
Specifically, the dried precipitate is obtained in the fourth step, and is pressed into blocks and cut into pieces for vacuum bagging.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A production process of food enzyme comprises 10-30 parts of edible corn flour, 40-50 parts of peanut meal, 5-7 parts of white granulated sugar, 6-7 parts of Aspergillus niger, 3-5 parts of calcium carbonate, 5-8 parts of Bacillus subtilis, 5-9 parts of glucose oxidase, 10-20 parts of yeast, 6-10 parts of an anticaking agent, 2-3 parts of an antibacterial agent, 3-10 parts of citric acid, 9-13 parts of microcrystalline cellulose and 10-30 parts of purified water, and the production process comprises the following steps:
the method comprises the following steps: crushing and mixing edible corn flour and peanut meal, putting the crushed and fused mixture into a fermentation container, adding pure water, wherein the ratio of the pure water to the mixture is 1:2 or 2:3, then continuously adding white granulated sugar, aspergillus niger, yeast, an anti-caking agent and an anti-microbial agent into the fermentation container, and after stirring, fermenting for 5-7 days at 30-60 ℃ to obtain fermentation liquor;
step two: taking out the fermentation liquor, performing filter pressing, and performing ultrafiltration concentration after filter pressing;
step three: concentrating, and precipitating with solvent;
step four: the precipitate obtained by precipitation was dried.
2. The process according to claim 1, wherein the enzyme is selected from the group consisting of: and step two, performing filter pressing through a plate frame, and performing ultrafiltration concentration through an ultrafiltration membrane.
3. The process according to claim 1, wherein the enzyme is selected from the group consisting of: and adding calcium carbonate, bacillus subtilis, glucose oxidase, citric acid and microcrystalline cellulose into the ultrafiltration concentrated solution obtained in the three-way step II, and stirring and mixing.
4. The process according to claim 1, wherein the enzyme is selected from the group consisting of: and D, precipitating the stirred mixed solution obtained in the step four, wherein the precipitated precipitate is enzyme mud, and the enzyme mud is air-dried or aired.
5. The process according to claim 1, wherein the enzyme is selected from the group consisting of: the temperature range for precipitation in step three is 8-11 ℃.
6. The process according to claim 1, wherein the enzyme is selected from the group consisting of: and step four, obtaining dried precipitate, briquetting, cutting and then carrying out vacuum bagging.
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103844266A (en) * | 2014-03-25 | 2014-06-11 | 容瑜 | Natural conditioning and nourishing ferment and manufacturing method thereof |
CN104542804A (en) * | 2014-12-31 | 2015-04-29 | 南通双和食品有限公司 | Food enzyme preparation and a preparation method thereof |
CN106820091A (en) * | 2016-12-13 | 2017-06-13 | 安徽翠鸟生物技术有限公司 | A kind of preparation method of food fermentation enzyme powder |
CN112450430A (en) * | 2020-12-16 | 2021-03-09 | 武汉伯森健康科技有限公司 | Preparation method of germinated brown rice jujube juice enzyme powder/tablet rich in natural digestive enzymes |
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2021
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Patent Citations (4)
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CN103844266A (en) * | 2014-03-25 | 2014-06-11 | 容瑜 | Natural conditioning and nourishing ferment and manufacturing method thereof |
CN104542804A (en) * | 2014-12-31 | 2015-04-29 | 南通双和食品有限公司 | Food enzyme preparation and a preparation method thereof |
CN106820091A (en) * | 2016-12-13 | 2017-06-13 | 安徽翠鸟生物技术有限公司 | A kind of preparation method of food fermentation enzyme powder |
CN112450430A (en) * | 2020-12-16 | 2021-03-09 | 武汉伯森健康科技有限公司 | Preparation method of germinated brown rice jujube juice enzyme powder/tablet rich in natural digestive enzymes |
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