CN113261475A - Tremella bassiana stock and preparation method thereof - Google Patents
Tremella bassiana stock and preparation method thereof Download PDFInfo
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- CN113261475A CN113261475A CN202110539707.XA CN202110539707A CN113261475A CN 113261475 A CN113261475 A CN 113261475A CN 202110539707 A CN202110539707 A CN 202110539707A CN 113261475 A CN113261475 A CN 113261475A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a linden seed of golden fungus and a preparation method thereof. The raw seed of the Tilia tilis is obtained by inoculating and culturing a culture medium prepared by taking fine crushed sawdust, wheat bran, sugar and gypsum as raw materials; the survival rate of the cultivation of the original seed of the linden wood of the golden fungus is more than 90 percent. The preparation method comprises the steps of pretreatment and inoculation culture. The survival rate of the basswood cultivation of the golden fungus seeds obtained by the inoculation cultivation of the invention reaches more than 90 percent, thus greatly improving the economic benefit of the cultivation of the golden fungus basswood; shortens the production period of the original golden fungus seeds and reduces the production cost.
Description
Technical Field
The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a linden original seed of tremella aurantialba and a preparation method thereof.
Background
Golden fungus (jin ErTremella aurantialba) It is named because it is golden yellow, and because the whole shape of the fruiting body is exactly like the brainTremella fuciformis, Tremella cerealis, etc., belonging to Basidiomycetes (Basidiomycetes), Tremellaceae (Tremellaceae), Tremella (Tremella)Tremella）。
The tremella aurantialba is a large-scale rare fungus which is both edible and medicinal, and has rich nutritive value and important medicinal value. The tremella aurantialba is rich in amino acids and proteins, contains 8 amino acids required by a human body, is rich in vitamins and minerals, contains a large amount of mineral elements in sporocarp, and also contains a small amount of elements such as iron, selenium and germanium besides a plurality of major elements such as calcium and potassium. The unique tremella aurantialba polysaccharide is one of the main active ingredients of tremella aurantialba, has various pharmacological activities of reducing blood sugar, resisting tumors, preventing and treating hyperlipidemia, resisting oxidation and the like, and is reported to have the effects of relieving cough, reducing phlegm and regulating qi. With the improvement of the requirements of people on food nutrition, the tremella aurantialba is used as a rare edible fungus with fine and smooth mouthfeel and fragrant taste for both food and medicine, and the market demand is increased year by year.
The annual yield of wild tremella aurantialba is low, and the growing consumer demand cannot be met. And the tremella aurantialba is a highlight in the edible fungus cultivation industry because of high nutritional value, high medicinal value and high economic value. Through more than ten years of domestication and cultivation, two cultivation modes of basswood cultivation and bag cultivation are developed. However, in the actual production process, the tremella aurantialba strains are poor in quality and uneven, and the phenomenon that many fake and invalid strains cannot grow mushroom normally often occurs after cultivation, so that the yield of tremella aurantialba fruiting bodies is not stable enough, and the industrialized cultivation development of tremella aurantialba is weak.
The pure tremella aurantialba hypha cannot generate sporocarp, the pure tremella aurantialba hypha has a parasitic or partial symbiotic relationship with the corious fungi such as stereum hirsutum, corious bacteria and the like, the proportion of the tremella aurantium hypha and the corious bacteria hypha is in a certain rule, and the sporocarp can be formed under the condition of proper environmental conditions, otherwise, only the hypha or the corious bacteria sporocarp can be formed in a culture medium, which is an important factor for causing ineffective strains and influencing the yield of the tremella aurantium sporocarp. In the artificial cultivation process, the ingredients of the culture material have great influence on the growth of tremella aurantialba hyphae and panus rudis hyphae, and the culture material used for cultivating the tremella aurantialba needs to simultaneously meet the growth conditions of the tremella aurantialba hyphae and the panus rudis hyphae, so that the proportion of the tremella aurantialba hyphae and the panus rudis hyphae tends to be stable, and the tremella aurantial.
Therefore, a scientific and reasonable formula of the culture material for the golden fungus artificial cultivation strain, which is simple to operate and easy to popularize, is needed to be made, so that the golden fungus can form a fruiting body, the phenomenon that the fruiting cannot be normally carried out due to invalid strains is reduced, and scientific guarantee is provided for large-scale artificial cultivation of the golden fungus.
Disclosure of Invention
The first purpose of the invention is to provide a linden seed of golden fungus; the second purpose is to provide the preparation method of the linden basswood stock.
The first purpose of the invention is realized by that the raw seed of the Tilia tiliana is obtained by inoculating and culturing a culture medium prepared by taking fine crushed sawdust, wheat bran, sugar and gypsum as raw materials; the survival rate of the cultivation of the original seed of the linden wood of the golden fungus is more than 90 percent.
The second purpose of the invention is realized by the following steps of pretreatment and inoculation culture, and the method specifically comprises the following steps:
A. pretreatment:
1) uniformly mixing the finely-ground sawdust, the wheat bran, the sugar and the gypsum according to the formula ratio, and adjusting the water content rate to 40-60% to obtain a culture medium a;
2) filling and compacting the culture medium a, drilling blind holes, sterilizing at high temperature and high pressure, and cooling for later use;
B. inoculating and culturing:
inoculating two young tremella aurantialba tissue blocks for culture, wherein the specific inoculation mode is as follows:
1) inoculating one of the culture bottles in the blind hole, and covering the blind hole with a culture medium a;
2) another piece is inoculated on the culture medium a above the blind hole;
3) culturing at 15-22 ℃ to obtain the target substance of the original seed of the Tilia amurensis.
The formula of the culture medium for culturing the tremella aurantialba protospecies is 83% of fine crushed sawdust (such as sawdust), 15% of wheat bran, 1% of sugar and 1% of gypsum. The advantages of sawdust-like wood chips: 1. the finely-crushed sawdust can absorb relatively much water, and the survival rate of the seeds cannot be influenced by water loss of mycelia due to water evaporation in a short time during inoculation; 2. the fine saw dust is easy to break, the fruit bodies can grow as long as the mixed hypha compost with the size of rice grains is used, a large amount of basswood can be inoculated, the seeds are saved, and the preparation cost of the seeds is reduced.
After the culture medium is bottled and compacted, a hole is punched in the center of the bottle by using a wood stick with the diameter of 1.5cm, but the hole cannot be punched to the bottom of the bottle. Sterilizing at high temperature and high pressure, and cooling.
When inoculating, the young tremella aurantialba mother seeds or protospecies are cut into tissue blocks with the size of 1-2cm, and 2 blocks are put into a bottle for culturing.
One piece of the seed is thrown into the seed hole of the culture bottle, the culture material is picked by a forceps to cover the seed hole, the inoculation method has 2 functions, the seed used for inoculating the seed on the linden is usually generated within 2cm of the contact surface of the tissue block and the culture medium, and because the contact surface of the tremella aurantialba tissue block and the culture material is the largest when the tremella aurantialba tissue block is expanded, a large amount of seeds for culturing the linden can be obtained; secondly, the contact surface is large, the fungus growing speed of the whole bottle is accelerated, and compared with the method for inoculating the tremella aurantialba tissue blocks on the surface of the culture medium, the inoculation method shortens the production time by about 5 days, saves the production cost and shortens the production period.
And placing the other piece on the surface of the culture medium. The method aims to facilitate the check of the survival of the original seed inoculation of the golden fungus and provide seeds for the grafting of the bag-cultivated golden fungus.
The invention particularly emphasizes the reason why the swelled fruit bodies inoculated in the holes can not be used as the golden fungus cultivation bags, because the surface of the golden fungus cultivation bags is stained with wood chips, and the quality of the golden fungus cultivation seeds can be affected after the fruit bodies grow up in the later period.
The invention has the beneficial effects that:
1. the method inoculates the golden fungus seeds obtained by culture, the survival rate of basswood cultivation reaches more than 90 percent, and the economic benefit of the golden fungus basswood cultivation is greatly improved;
2. the production period of the original golden fungus seeds is shortened, and the production cost is reduced;
3. can inoculate the Tilia miqueliana Maxim and can inoculate the bag material of the Tremella miqueliana Maxim, thereby having two purposes and enlarging the production range.
Drawings
Fig. 1 is a schematic view of the survival rate of the original seed of linden amurensis of tremella aurantialba according to embodiment 1 of the present invention.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to be limiting in any way, and any modifications or alterations based on the teachings of the present invention are intended to fall within the scope of the present invention.
The raw seed of the Tilia amurensis is obtained by inoculating and culturing a culture medium prepared by taking fine crushed sawdust, wheat bran, sugar and gypsum as raw materials; the survival rate of the cultivation of the original seed of the linden wood of the golden fungus is more than 90 percent.
The culture material comprises 80-85 parts of fine crushed sawdust, 10-20 parts of wheat bran, 0.8-1.2 parts of sugar and 0.8-1.2 parts of gypsum by weight.
The preparation method of the tilia amurensis stock seed comprises the steps of pretreatment and inoculation culture, and specifically comprises the following steps:
A. pretreatment:
1) uniformly mixing the finely-ground sawdust, the wheat bran, the sugar and the gypsum according to the formula ratio, and adjusting the water content rate to 40-60% to obtain a culture medium a;
2) filling and compacting the culture medium a, drilling blind holes, sterilizing at high temperature and high pressure, and cooling for later use;
B. inoculating and culturing:
inoculating two young tremella aurantialba tissue blocks for culture, wherein the specific inoculation mode is as follows:
1) inoculating one of the culture bottles in the blind hole, and covering the blind hole with a culture medium a;
2) another piece is inoculated on the culture medium a above the blind hole;
3) culturing at 15-22 ℃ to obtain the target substance of the original seed of the Tilia amurensis.
The depth of the blind hole in the step A2) is 1.5-3 cm.
The aperture of the blind hole in the step A) is 1-2 cm.
The size of the tender tremella aurantialba tissue blocks is 0.8-1.5 cm.
The invention is further illustrated by the following specific examples:
example 1
1) Weighing 800g of fine crushed sawdust, 100g of wheat bran, 8g of sugar and 8g of gypsum according to the weight ratio, uniformly mixing, and adjusting the water content rate to be 60% to obtain a culture medium a;
2) filling the culture medium a into bottles, compacting, drilling blind holes with the depth of 1.5cm and the aperture of 1.0cm, sterilizing at high temperature and high pressure, and cooling for later use;
B. inoculating and culturing:
inoculating two tender tremella aurantialba tissue blocks with the sizes of 0.8cm and 1.1cm respectively for culture, wherein the specific inoculation mode is as follows:
1) inoculating a tender tremella aurantialba tissue block with the size of 0.8cm into the blind hole of the culture bottle, and then covering the blind hole with a culture medium a;
2) inoculating 1.1cm tender Tremella aurantialba tissue blocks on the culture medium a above the blind holes;
3) culturing at 15 deg.C for 15 days to obtain target substance of Tilia tiliana original seed.
The survival rate of the linden cultivation of the original seed of linden amurensis prepared in example 1 is 98.8%.
Example 2
1) Weighing 850g of fine crushed sawdust, 200g of wheat bran, 12g of sugar and 12g of gypsum according to the weight ratio, uniformly mixing, and adjusting the water content rate to 40% to obtain a culture medium a;
2) filling and compacting the culture medium a, drilling blind holes with the depth of 3cm and the aperture of 2.0cm, and sterilizing and cooling at high temperature and high pressure for later use;
B. inoculating and culturing:
inoculating two tender tremella aurantialba tissue blocks with the sizes of 1.3cm and 1.5cm respectively for culture, wherein the specific inoculation mode is as follows:
1) inoculating a 1.3 cm-sized tender tremella aurantialba tissue block into the blind hole of the culture bottle, and then covering the blind hole with a culture medium a;
2) inoculating 1.5cm tender Tremella aurantialba tissue blocks on the culture medium a above the blind holes;
3) culturing at 22 deg.C for 14 days in the dark to obtain target substance of Tilia tiliana original seed.
The survival rate of the linden cultivation of the original seed of the linden of golden fungus prepared in the example 2 is 97.6%.
Example 3
1) Weighing 830g of fine crushed sawdust, 150g of wheat bran, 10g of sugar and 10g of gypsum according to the weight ratio, uniformly mixing, and adjusting the water content rate to 50% to obtain a culture medium a;
2) filling the culture medium a into bottles, compacting, drilling blind holes with the depth of 2.0cm and the aperture of 1.5cm, sterilizing at high temperature and high pressure, and cooling for later use;
B. inoculating and culturing:
inoculating two tender tremella aurantialba tissue blocks with the sizes of 0.9cm and 1.2cm respectively for culture, wherein the specific inoculation mode is as follows:
1) inoculating a tender tremella aurantialba tissue block with the size of 0.9cm into the blind hole of the culture bottle, and then covering the blind hole with a culture medium a;
2) inoculating 1.2cm tender Tremella aurantialba tissue blocks on the culture medium a above the blind holes;
3) culturing at 18 deg.C in the dark for 12 days to obtain target substance of Tilia tiliana original seed.
The survival rate of the linden culture of the original seed of the linden of golden fungus prepared in the example 3 is 99.3%.
Claims (6)
1. A Tilia tiliana stock seed is characterized in that the Tilia tiliana stock seed is obtained by inoculating and culturing a culture medium prepared by taking fine crushed sawdust, wheat bran, sugar and gypsum as raw materials; the survival rate of the cultivation of the original seed of the linden wood of the golden fungus is more than 90 percent.
2. The tilia amurensis raw seed as claimed in claim 1, characterized in that the culture medium is composed of 80-85 parts by weight of fine crushed sawdust, 10-20 parts by weight of wheat bran, 0.8-1.2 parts by weight of sugar and 0.8-1.2 parts by weight of gypsum.
3. The preparation method of tilia amurensis stock species according to claim 1 or 2, characterized by comprising the steps of pretreatment and inoculation culture, and specifically comprising:
A. pretreatment:
1) uniformly mixing the finely-ground sawdust, the wheat bran, the sugar and the gypsum according to the formula ratio, and adjusting the water content rate to 40-60% to obtain a culture medium a;
2) filling and compacting the culture medium a, drilling blind holes, sterilizing at high temperature and high pressure, and cooling for later use;
B. inoculating and culturing:
inoculating two young tremella aurantialba tissue blocks for culture, wherein the specific inoculation mode is as follows:
1) inoculating one of the culture bottles in the blind hole, and covering the blind hole with a culture medium a;
2) another piece is inoculated on the culture medium a above the blind hole;
3) dark culture is carried out at the temperature of 15-22 ℃ to obtain the target substance of the original seed of the Tilia amurensis.
4. The preparation method according to claim 3, wherein the depth of the blind hole in step A2) is 1.5-3 cm.
5. The preparation method according to claim 3, wherein the diameter of the blind hole in step A2) is 1-2 cm.
6. The method according to claim 3, wherein the size of the tender Tremella aurantialba tissue mass is 0.8-1.5 cm.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106105773A (en) * | 2016-06-24 | 2016-11-16 | 四川省大真科技有限责任公司 | The producing method for seed of edible mushroom cultivated species and prepared cultigen and cultivating method for edible fungi |
| CN109566268A (en) * | 2018-12-10 | 2019-04-05 | 中华全国供销合作总社昆明食用菌研究所 | Effective original seed of a kind of gold ear and preparation method thereof |
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- 2021-05-18 CN CN202110539707.XA patent/CN113261475A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106105773A (en) * | 2016-06-24 | 2016-11-16 | 四川省大真科技有限责任公司 | The producing method for seed of edible mushroom cultivated species and prepared cultigen and cultivating method for edible fungi |
| CN109566268A (en) * | 2018-12-10 | 2019-04-05 | 中华全国供销合作总社昆明食用菌研究所 | Effective original seed of a kind of gold ear and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| 丁文奇: "两点接菌法", 食用菌, no. 04, pages 10 * |
| 刘平: "金耳段木栽培试验简报", 食用菌, no. 03, pages 19 * |
| 刘正南等: "金耳的生理特性及有效优良菌种的制备原理", 中国食用菌, vol. 14, no. 05, pages 10 - 11 * |
| 刘正南等: "金耳的生理特性及有效优良菌种的制备原理（续）", 中国食用菌, vol. 14, no. 06, pages 9 - 11 * |
| 魏鹏: "新疆食用菌栽培技术(七)", 农村科技, no. 07, pages 86 - 87 * |
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