CN113243526A - Edible bird's nest acid extraction method for edible bird's nest processing - Google Patents
Edible bird's nest acid extraction method for edible bird's nest processing Download PDFInfo
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- CN113243526A CN113243526A CN202110488428.5A CN202110488428A CN113243526A CN 113243526 A CN113243526 A CN 113243526A CN 202110488428 A CN202110488428 A CN 202110488428A CN 113243526 A CN113243526 A CN 113243526A
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- cubilose
- acid
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- methanol solution
- edible bird
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- 239000002253 acid Substances 0.000 title claims abstract description 124
- 244000000626 Daucus carota Species 0.000 title claims abstract description 88
- 235000005770 birds nest Nutrition 0.000 title claims abstract description 88
- 235000005765 wild carrot Nutrition 0.000 title claims abstract description 88
- 238000000605 extraction Methods 0.000 title abstract description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 375
- 150000005846 sugar alcohols Chemical class 0.000 claims abstract description 37
- 239000000843 powder Substances 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 24
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 24
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 24
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims abstract description 23
- 238000010438 heat treatment Methods 0.000 claims abstract description 23
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims abstract description 20
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims abstract description 20
- 239000001257 hydrogen Substances 0.000 claims abstract description 17
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 17
- 239000008367 deionised water Substances 0.000 claims abstract description 15
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims description 19
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- 238000004108 freeze drying Methods 0.000 claims description 10
- 229920001542 oligosaccharide Polymers 0.000 claims description 9
- 150000002482 oligosaccharides Chemical class 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 7
- 239000007789 gas Substances 0.000 claims description 5
- 150000002431 hydrogen Chemical class 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 abstract description 3
- 239000002131 composite material Substances 0.000 abstract description 3
- 229910001431 copper ion Inorganic materials 0.000 abstract description 3
- 229960003280 cupric chloride Drugs 0.000 abstract description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 3
- 229910052742 iron Inorganic materials 0.000 abstract description 3
- -1 iron ions Chemical class 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 2
- 230000000050 nutritive effect Effects 0.000 abstract description 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 abstract 1
- 239000000047 product Substances 0.000 description 9
- 229920001223 polyethylene glycol Polymers 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- 239000012535 impurity Substances 0.000 description 4
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 125000000468 ketone group Chemical group 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/015—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
- A23L29/035—Organic compounds containing oxygen as heteroatom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a method for extracting edible bird's nest acid for processing edible bird's nests, and relates to the technical field of foods. Firstly, pretreating cubilose, dispersing cubilose powder in deionized water, ventilating and heating, adding methanol solution for three times, and finally centrifuging to obtain methanol solution containing free cubilose acid on the upper layer and methanol cubilose gel containing combined cubilose acid on the lower layer; adding ferric chloride and cupric chloride to the lower layer, heating, adding methanol solution for three times while introducing hydrogen, reacting, centrifuging to obtain methanol solution containing protein-bound edible bird's nest acid sugar alcohol, and mixing the two to obtain composite edible bird's nest acid containing free edible bird's nest acid and protein-bound edible bird's nest acid sugar alcohol. The invention improves the extraction rate of the cubilose acid, and simultaneously the prepared composite cubilose acid which contains the cubilose acid and the cubilose acid sugar alcohol and contains a small amount of iron ions and copper ions has high nutritive value, and the cubilose product is prepared and is more suitable for being eaten by diabetics.
Description
Technical Field
The invention relates to the technical field of food, in particular to a method for extracting edible bird's nest acid for processing edible bird's nests.
Background
At present, the cubilose acid in the market can be processed and extracted by using cubilose, but in actual use, in the extraction process of the cubilose acid, a hydrolysis mode, acidolysis or enzymolysis is adopted, so that impurity invasion is easily caused while the cubilose acid is extracted, the later separation is inconvenient, the quality of the cubilose acid is influenced, the processing speed is slow, the processing period is prolonged due to the large-scale processing requirement, the waste of capital is caused, and the problems are actually existed and urgently need to be solved.
Sialic acid is predominant in 3 states in organisms: the protein binding state, the oligosaccharide binding state and the free state, wherein the protein binding state and the oligosaccharide binding state are mainly existing states, the cubilose acid in the oligosaccharide binding state is difficult to absorb, and the cubilose acid in the protein binding state is difficult to extract, so that the cubilose acid in the cubilose is wasted greatly.
Disclosure of Invention
The invention aims to provide a method for extracting edible bird's nest acid for processing edible bird's nests, which aims to solve the problems in the background technology.
In order to solve the above technical problem, a first aspect of the present invention provides the following technical solutions: the method for extracting the edible bird's nest acid for processing the edible bird's nests is characterized in that the process flow for extracting the edible bird's nest acid for processing the edible bird's nests is as follows:
pretreating, preparing a methanol solution containing free-state cubilose acid, preparing a methanol solution containing protein-bound-state cubilose acid sugar alcohol, and preparing cubilose acid.
Preferably, the method comprises the following specific steps:
(1) drying the cubilose in an electric oven to dehydrate and crisp the cubilose;
(2) crushing the dried edible bird's nest by using a crusher to obtain edible bird's nest powder;
(3) dispersing cubilose powder in deionized water, uniformly stirring, ventilating and heating, heating for 20-30 min, adding methanol solution for three times, uniformly stirring, centrifuging, obtaining methanol solution containing free cubilose acid on the upper layer, and obtaining methanol cubilose gel containing combined cubilose acid on the lower layer;
(4) adding ferric chloride and copper chloride into the methanol cubilose gel containing the combined-state cubilose acid on the lower layer, heating, introducing hydrogen after 20-30 min, adding methanol solution for three times, and stopping introducing the hydrogen when gas in the gel continuously escapes;
(5) stopping introducing hydrogen, centrifuging to obtain methanol solution containing protein-bound nidus Collocaliae sugar alcohol in the upper layer, and methanol nidus Collocaliae gel containing oligosaccharide-bound nidus Collocaliae sugar alcohol in the lower layer;
(6) mixing methanol solution containing protein-bound cubilose acid sugar alcohol and methanol solution containing free cubilose acid uniformly according to a certain volume ratio, freeze-drying, washing with absolute ethyl alcohol after drying, and drying to obtain the finished product.
Preferably, in the step (3): the mass ratio of the cubilose powder to the deionized water to the methanol is 1: 1: 3; the aeration rate is 0.2-0.5L/min, and the mixture is heated to 80-100 ℃; the centrifugation condition is 2000-4000 rpm, and the time is 10-30 min.
Preferably, in the step (3): the methanol solution added in each time is 1/3 the total volume of the methanol solution; the mass fraction of the methanol solution is 40-60%.
Preferably, in the step (4): the mass ratio of the cubilose powder, the ferric chloride, the copper chloride and the methanol solution is 20: 2: 1: 10-20: 5: 2: 10; the mass fraction of the methanol solution is 40-60%.
Preferably, in the step (4): the methanol solution added in each time is 1/3 the total volume of the methanol solution; the rate of introducing hydrogen is 0.2-0.5L/min.
Preferably, in the step (5): the centrifugation condition is 1000-2000 rpm, and the time is 20-40 min.
Preferably, in the step (6): the volume ratio of the methanol solution containing protein-bound cubilose acid sugar alcohol to the methanol solution containing free cubilose acid is 1: 1-1: 3, mixing; the freeze drying temperature is-45 to-55 ℃, the pressure is 20 to 50Pa, and the time is 24 to 48 hours.
The second aspect of the invention is an extraction method of edible bird's nest acid for processing edible bird's nests, which is characterized in that the edible bird's nest acid for processing edible bird's nests, which is prepared by the extraction method of edible bird's nest acid for processing edible bird's nests, comprises the following raw materials in parts by weight: 20-40 parts of cubilose, 2-10 parts of ferric chloride, 1-4 parts of copper chloride and 80-200 parts of methanol solution; the mass fraction of the methanol solution is 40-60%.
Compared with the prior art, the invention has the following beneficial effects:
when the bird's nest is pretreated, the bird's nest is dried and crushed to obtain bird's nest powder, then the bird's nest powder is dispersed in deionized water, ventilated and heated, methanol solution is added in three times, the mixture is evenly stirred and then centrifuged, the methanol solution containing free bird's nest acid is obtained at the upper layer while impurities are directly removed, and methanol bird's nest gel containing combined bird's nest acid is obtained at the lower layer; the cubilose powder is dispersed in deionized water and then is aerated and heated, and methanol is gradually added to denature protein in the cubilose and further separate from free cubilose acid, so that the cubilose acid is not only accelerated to be dissolved in the deionized water, but also the free cubilose acid is completely separated from water-soluble protein, impurities, polysaccharide and the like in the cubilose, and the methanol solution containing the free cubilose acid with higher purity is obtained, the introduction of the impurities is reduced, the process is simplified, and the extraction rate of the cubilose acid is improved.
Adding ferric chloride and copper chloride into the lower methanol nidus Collocaliae gel containing combined-state nidus Collocaliae acid, heating, introducing hydrogen for a period of time, adding methanol solution for three times, centrifuging after reaction, obtaining methanol solution containing protein-combined-state nidus Collocaliae acid sugar alcohol at the upper layer, and obtaining methanol nidus Collocaliae gel containing oligosaccharide-combined-state nidus Collocaliae acid sugar alcohol at the lower layer; uniformly mixing a methanol solution containing protein-bound cubilose acid sugar alcohol with a methanol solution containing free cubilose acid, freeze-drying, washing with ethanol after drying, and drying to obtain composite cubilose acid containing the free cubilose acid and the protein-bound cubilose acid sugar alcohol; transferring ketone group on protein-bound cubilose acid in cubilose to hydroxyl group by adding ferric chloride and cupric chloride, converting into cubilose acid sugar alcohol and transferring into methanol solvent, and then removing iron ions and copper ions through excessive hydrogen; after freeze drying, the methanol is completely washed away by using ethanol, so that the product is the compound cubilose acid which is cubilose acid and cubilose acid sugar alcohol and contains a small amount of iron ions and copper ions, the extraction rate of the cubilose acid is improved, the cubilose acid in an oligosaccharide combined state which is difficult to absorb is abandoned, the nutritive value is high, and the cubilose product is prepared and is more suitable for being eaten by diabetes patients.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to more clearly illustrate the method provided by the invention, the following examples are used for detailed description, and the method for testing each index of the edible bird's nest acid for edible bird's nest processing extracted in the following examples is as follows:
and (3) detecting the content of the cubilose acid and the content and purity of cubilose acid sugar alcohol: the content of the cubilose acid and the content and purity of the cubilose acid sugar alcohol refer to the second part of the Chinese pharmacopoeia of 2015 edition.
Example 1
The edible bird's nest acid for processing edible bird's nests mainly comprises the following components in parts by weight:
20 parts of cubilose, 2 parts of ferric chloride, 1 part of copper chloride and 80 parts of methanol solution; the mass fraction of the methanol solution is 40%.
The method for extracting the edible bird's nest acid for processing the edible bird's nests comprises the following steps:
(1) drying the cubilose in an electric oven to dehydrate and crisp the cubilose;
(2) crushing the dried edible bird's nest by using a crusher to obtain edible bird's nest powder;
(3) dispersing cubilose powder in deionized water, stirring uniformly, ventilating and heating at the ventilating rate of 0.2L/min, heating to 80 ℃, heating for 20min, adding methanol solution for three times, wherein the methanol solution added each time is 1/3 of the total volume of the methanol solution, and the mass ratio of the cubilose powder to the deionized water to the methanol is 1: 1: 3; centrifuging after stirring uniformly, wherein the centrifuging condition is 2000rpm, the time is 15min, the upper layer obtains a methanol solution containing free-state cubilose acid, and the lower layer obtains methanol cubilose gel containing combined-state cubilose acid;
(4) adding ferric chloride and copper chloride into the lower methanol cubilose gel containing the combined cubilose acid, heating, introducing hydrogen after 20min, and adding methanol solution for three times, wherein the mass ratio of cubilose powder, ferric chloride, copper chloride and methanol solution is 20: 2: 1: 20, the methanol solution added each time is 1/3 of the total volume of the methanol solution, the hydrogen gas introduction rate is 0.2L/min, and the hydrogen gas introduction is stopped when the gas in the gel continuously escapes;
(5) stopping introducing hydrogen, centrifuging at 1000rpm for 20min to obtain methanol solution containing protein-bound nidus Collocaliae alditol in the upper layer and methanol nidus Collocaliae gel containing oligosaccharide-bound nidus Collocaliae alditol in the lower layer;
(6) mixing a methanol solution containing protein-bound cubilose acid sugar alcohol and a methanol solution containing free cubilose acid according to a volume ratio of 1: 1, uniformly mixing, freeze-drying at-45 ℃ under 20Pa for 24 hours, washing with absolute ethyl alcohol after drying, and drying to obtain the finished product.
Example 2
The edible bird's nest acid for processing edible bird's nests mainly comprises the following components in parts by weight:
40 parts of cubilose, 10 parts of ferric chloride, 4 parts of copper chloride and 200 parts of methanol solution; the mass fraction of the methanol solution is 60%.
The method for extracting the edible bird's nest acid for processing the edible bird's nests comprises the following steps:
(1) drying the cubilose in an electric oven to dehydrate and crisp the cubilose;
(2) crushing the dried edible bird's nest by using a crusher to obtain edible bird's nest powder;
(3) dispersing cubilose powder in deionized water, stirring uniformly, ventilating and heating at the ventilating rate of 0.5L/min, heating to 100 ℃, adding methanol solution for three times after heating for 30min, wherein the methanol solution added each time is 1/3 of the total volume of the methanol solution, and the mass ratio of the cubilose powder to the deionized water to the methanol is 1: 1: 3; stirring uniformly, centrifuging at 4000rpm for 25min to obtain methanol solution containing free nidus Collocaliae acid at the upper layer and methanol nidus Collocaliae gel containing bound nidus Collocaliae acid at the lower layer;
(4) adding ferric chloride and copper chloride into methanol cubilose gel containing combined-state cubilose acid at the lower layer, heating, introducing hydrogen after 30min, and adding methanol solution for three times, wherein the mass ratio of cubilose powder, ferric chloride, copper chloride and methanol solution is 20: 5: 5: 20, the methanol solution added each time is 1/3 of the total volume of the methanol solution, the hydrogen gas introduction rate is 0.5L/min, and the hydrogen gas introduction is stopped when the gas in the gel continuously escapes;
(5) stopping introducing hydrogen, centrifuging at 2000rpm for 40min to obtain methanol solution containing protein-bound nidus Collocaliae alditol in the upper layer and methanol nidus Collocaliae gel containing oligosaccharide-bound nidus Collocaliae alditol in the lower layer;
(6) mixing a methanol solution containing protein-bound cubilose acid sugar alcohol and a methanol solution containing free cubilose acid according to a volume ratio of 1: 3, uniformly mixing, freeze-drying at-55 ℃ under 50Pa for 48 hours, washing with absolute ethyl alcohol after drying, and drying to obtain the finished product.
Comparative example 1
The edible bird's nest acid for processing edible bird's nests mainly comprises the following components in parts by weight:
20 parts of cubilose and 80 parts of methanol solution; the mass fraction of the methanol solution is 40%.
The method for extracting the edible bird's nest acid for processing the edible bird's nests comprises the following steps:
(1) drying the cubilose in an electric oven to dehydrate and crisp the cubilose;
(2) crushing the dried edible bird's nest by using a crusher to obtain edible bird's nest powder;
(3) dispersing cubilose powder in deionized water, stirring uniformly, ventilating and heating at the ventilating rate of 0.2L/min, heating to 80 ℃, heating for 20min, adding methanol solution for three times, wherein the methanol solution added each time is 1/3 of the total volume of the methanol solution, and the mass ratio of the cubilose powder to the deionized water to the methanol is 1: 1: 3; centrifuging after stirring uniformly, wherein the centrifuging condition is 2000rpm, the time is 15min, the upper layer obtains a methanol solution A containing free-state cubilose acid, and the lower layer obtains methanol cubilose gel containing combined-state cubilose acid;
(4) adding methanol solution into the methanol bird's nest gel containing the binding-state bird's nest acid in the lower layer for three times, wherein the mass ratio of the bird's nest powder to the methanol solution is 1: 1/3, wherein the methanol solution added in each time is the total volume of the methanol solution;
(5) after the reaction is finished, centrifuging for 20min at 1000rpm, wherein the upper layer is a methanol solution B containing free-state bird's nest acid, and the lower layer is a methanol bird's nest gel containing combined-state bird's nest acid;
(6) mixing the solution A and the solution B according to a volume ratio of 1: 1, uniformly mixing, freeze-drying at-45 ℃ under 20Pa for 24 hours, washing with absolute ethyl alcohol after drying, and drying to obtain the finished product.
Comparative example 2
The edible bird's nest acid for processing edible bird's nests mainly comprises the following components in parts by weight:
20 parts of cubilose, 2 parts of ferric chloride, 1 part of copper chloride and 80 parts of methanol solution; the mass fraction of the methanol solution is 40%.
The method for extracting the edible bird's nest acid for processing the edible bird's nests comprises the following steps:
(1) drying the cubilose in an electric oven to dehydrate and crisp the cubilose;
(2) crushing the dried edible bird's nest by using a crusher to obtain edible bird's nest powder;
(3) dispersing nidus Collocaliae powder in deionized water, stirring well, ventilating and heating at a speed of 0.2L/min to 80 deg.C, heating for 20min, and adding methanol solution three times to obtain methanol nidus Collocaliae gel containing nidus Collocaliae acid;
(4) adding ferric chloride and copper chloride into methanol cubilose gel containing combined-state cubilose acid, heating, introducing hydrogen after 20min, and adding methanol solution for three times, wherein the mass ratio of cubilose powder, ferric chloride, copper chloride and methanol solution is 20: 2: 1: 20, the methanol solution added each time is 1/3 of the total volume of the methanol solution, the hydrogen gas introduction rate is 0.2L/min, and the hydrogen gas introduction is stopped when the gas in the gel continuously escapes;
(5) stopping introducing hydrogen, centrifuging at 1000rpm for 20min to obtain methanol solution containing protein-bound nidus Collocaliae alditol in the upper layer and methanol nidus Collocaliae gel containing oligosaccharide-bound nidus Collocaliae alditol in the lower layer;
(6) freeze-drying methanol solution containing protein-bound cubilose acid sugar alcohol at-45 deg.C under 20Pa for 24 hr, washing with anhydrous ethanol, and drying to obtain the final product.
Examples of effects
Table 1 below shows the results of the analyses of the contents of sialic acid, sugar alcohol and purity, which were measured in examples 1 and 2 and comparative examples 1 and 2, respectively, using the present invention.
TABLE 1
By comparing the contents of the cubilose acid and the cubilose acid sugar alcohol in the examples 1 and 2 and the comparative examples 1 and 2, the fact that the cubilose acid sugar alcohol in the examples 1 and 2 is prepared to be very suitable for being eaten by the diabetics after the pretreatment is carried out, and part of the combined cubilose acid can be converted into the cubilose acid sugar alcohol to be extracted by adding the ferric chloride and the copper chloride, and the cubilose acid sugar alcohol content in the product is higher than that of the cubilose acid, so that the cubilose acid extraction rate is improved and the cubilose is very suitable for being eaten by the diabetics; by comparing the purity of the cubilose acid and the cubilose acid sugar alcohol of the examples 1 and 2 with that of the comparative examples 1 and 2, the fact that the products prepared by the pretreatment of the examples 1 and 2 and the addition of the ferric chloride and the cupric chloride can convert part of the combined cubilose acid into the cubilose acid sugar alcohol have higher purity and have higher nutritional value when being prepared into edible cubilose products can be obviously found.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The method for extracting the edible bird's nest acid for processing the edible bird's nests is characterized in that the process flow for extracting the edible bird's nest acid for processing the edible bird's nests is as follows: pretreating, preparing a methanol solution containing free-state cubilose acid, preparing a methanol solution containing protein-bound-state cubilose acid sugar alcohol, and preparing cubilose acid.
2. The method for extracting the cubilose acid for processing the edible cubilose according to claim 1, which is characterized by comprising the following specific steps of:
(1) drying the cubilose in an electric oven to dehydrate and crisp the cubilose;
(2) crushing the dried edible bird's nest by using a crusher to obtain edible bird's nest powder;
(3) dispersing cubilose powder in deionized water, uniformly stirring, ventilating and heating, heating for 20-30 min, adding methanol solution for three times, uniformly stirring, centrifuging, obtaining methanol solution containing free cubilose acid on the upper layer, and obtaining methanol cubilose gel containing combined cubilose acid on the lower layer;
(4) adding ferric chloride and copper chloride into the methanol cubilose gel containing the combined-state cubilose acid on the lower layer, heating, introducing hydrogen after 20-30 min, adding methanol solution for three times, and stopping introducing the hydrogen when gas in the gel continuously escapes;
(5) stopping introducing hydrogen, centrifuging to obtain methanol solution containing protein-bound nidus Collocaliae sugar alcohol in the upper layer, and methanol nidus Collocaliae gel containing oligosaccharide-bound nidus Collocaliae sugar alcohol in the lower layer;
(6) mixing methanol solution containing protein-bound cubilose acid sugar alcohol and methanol solution containing free cubilose acid uniformly according to a certain volume ratio, freeze-drying, washing with absolute ethyl alcohol after drying, and drying to obtain the finished product.
3. The method for extracting edible bird's nest acid used for edible bird's nest processing according to claim 2, characterized in that, in the step (3): the mass ratio of the cubilose powder to the deionized water to the methanol is 1: 1: 3; the aeration rate is 0.2-0.5L/min, and the mixture is heated to 80-100 ℃; the centrifugation condition is 2000-4000 rpm, and the time is 10-30 min.
4. The method for extracting edible bird's nest acid used for edible bird's nest processing according to claim 2, characterized in that, in the step (3): the methanol solution added in each time is 1/3 the total volume of the methanol solution; the mass fraction of the methanol solution is 40-60%.
5. The method for extracting edible bird's nest acid according to claim 2, characterized in that, in the step (4): the mass ratio of the cubilose powder, the ferric chloride, the copper chloride and the methanol solution is 20: 2: 1: 10-20: 5: 2: 10; the mass fraction of the methanol solution is 40-60%.
6. The method for extracting edible bird's nest acid used for edible bird's nest processing according to claim 2, characterized in that, in the step (4): the methanol solution added in each time is 1/3 the total volume of the methanol solution; the rate of introducing hydrogen is 0.2-0.5L/min.
7. The method for extracting edible bird's nest acid used for edible bird's nest processing according to claim 2, characterized in that, in the step (5): the centrifugation condition is 1000-2000 rpm, and the time is 20-40 min.
8. The method for extracting edible bird's nest acid used for edible bird's nest processing according to claim 2, characterized in that, in the step (6): the volume ratio of the methanol solution containing protein-bound cubilose acid sugar alcohol to the methanol solution containing free cubilose acid is 1: 1-1: 3, mixing; the freeze drying temperature is-45 to-55 ℃, the pressure is 20 to 50Pa, and the time is 24 to 48 hours.
9. The method for extracting the edible bird's nest acid for edible bird's nest processing according to claim 1, wherein the bird's nest acid for edible bird's nest processing extracted by the method for extracting the edible bird's nest acid for edible bird's nest processing comprises the following raw materials in parts by weight: 20-40 parts of cubilose, 2-10 parts of ferric chloride, 1-4 parts of copper chloride and 80-200 parts of methanol solution; the mass fraction of the methanol solution is 40-60%.
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