CN113210027B - Water bath method activity extraction equipment for placenta stem cell extract and use method - Google Patents

Water bath method activity extraction equipment for placenta stem cell extract and use method Download PDF

Info

Publication number
CN113210027B
CN113210027B CN202110512755.XA CN202110512755A CN113210027B CN 113210027 B CN113210027 B CN 113210027B CN 202110512755 A CN202110512755 A CN 202110512755A CN 113210027 B CN113210027 B CN 113210027B
Authority
CN
China
Prior art keywords
water bath
fixedly connected
wire
water
controller
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202110512755.XA
Other languages
Chinese (zh)
Other versions
CN113210027A (en
Inventor
宋正宝
普春芬
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sichuan Wuyan Biotechnology Co ltd
Original Assignee
Sichuan Wuyan Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sichuan Wuyan Biotechnology Co ltd filed Critical Sichuan Wuyan Biotechnology Co ltd
Priority to CN202110512755.XA priority Critical patent/CN113210027B/en
Publication of CN113210027A publication Critical patent/CN113210027A/en
Application granted granted Critical
Publication of CN113210027B publication Critical patent/CN113210027B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/02Water baths; Sand baths; Air baths
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00Supporting devices; Holding devices
    • B01L9/06Test-tube stands; Test-tube holders
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/18Means for temperature control
    • B01L2300/1805Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Medical Bathing And Washing (AREA)

Abstract

The invention relates to the technical field of cell processing devices and discloses a water bath method active extraction device for placenta stem cell extracts and a use method thereof.

Description

Water bath method activity extraction equipment for placenta stem cell extract and use method
Technical Field
The invention relates to the technical field of cell treatment devices, in particular to a water bath method activity extraction device for placenta stem cell extracts and a use method thereof.
Background
The placenta stem cells found scientifically at present contain: placental mesenchymal stem cells, placental sub-totipotent stem cells, placental hematopoietic stem cells, which are stem cells obtained by collection of placental tissue, and when neonates are born, stem cells can be generally obtained through three channels: the first is to collect hematopoietic stem cells for the blood source in the umbilical cord; the second is to collect umbilical cord source mesenchymal stem cells in the umbilical cord itself; and thirdly, collecting placenta stem cells for placenta sources. In the clinical application prospect, the placenta source stem cells have important roles, have infinite prospect in the fields of autologous organ repair, clone transplantation, cosmetology, hereditary disease treatment, heart disease, liver disease, diabetes mellitus and the like, the placenta stem cells are mostly extracted from corresponding placenta extracts in the prior art, and the stem cell extracts under the refrigeration condition need to be heated when the stem cells are extracted from the extracts, so that the activity of the stem cells in the extracts is improved, and the reaction speed with the decomposed biological enzymes is accelerated.
In the prior art, the water bath method is adopted when the conventional placenta stem cell extract is extracted, and the manual clamping test tube is used for heating to extract the activity, so that the method is time-consuming and labor-consuming and can easily cause the occurrence of unexpected situations such as test tube falling off, and on the other hand, most of heating and heating mechanisms only locally heat the aqueous solution, so that the water temperature of each region in the aqueous solution is large in the same time difference, the whole environment temperature of the aqueous solution cannot be accurately controlled, and then the stem cell structure is easily damaged, so that the stem cells are invalid.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides water bath extraction activity equipment for placenta stem cell extracts, which has the advantages of accurately controlling water temperature, avoiding uneven water temperature and the like, and solves the problems that unexpected situations such as test tube falling and the like are easily caused by heating and extracting activity in a manual test tube clamping mode, and the water temperature of each region in the water solution is large in the same time difference due to the fact that most heating and heating mechanisms only locally heat the water solution, so that the overall environment temperature of the water solution cannot be accurately controlled.
(II) technical scheme
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a placenta stem cell extract water bath method draws active equipment, includes the water bath, annular seal groove has been seted up at the top of water bath, be equipped with inlayer heat preservation sponge in the annular seal groove, four evenly distributed's rectangle stopper of one side inner wall fixedly connected with of water bath, one side inner wall fixedly connected with water bath baffle and gear baffle of water bath, the position of water bath baffle is four the position of rectangle stopper is corresponding, and four the bottom of rectangle stopper all with the water bath baffle is connected, the water bath baffle with the one end that gear baffle is close to each other is equipped with same sealed chamber, the gear baffle with the bottom inner wall of water bath is equipped with same gear chamber, four rectangle stopper all has been seted up to one side that rectangle stopper is close to each other, four a plurality of evenly distributed's circulation holes have all been seted up to one side of rectangle stopper, a plurality of circulation holes respectively with corresponding four rectangle stopper communicates each other, be equipped with the test tube anchor clamps in the water bath, fixedly connected with limiting plate and board in the water bath anchor clamps, the test tube is located the top of limiting plate water bath board, a plurality of evenly distributed water bath holes are seted up to water bath one end.
Preferably, four rectangular engaging blocks which are uniformly distributed are fixedly connected to the outer side of the test tube clamp, the position chalks of the four rectangular engaging blocks correspond to the positions of the four rectangular limiting grooves and are matched with the positions of the four rectangular limiting grooves, the four rectangular engaging blocks are respectively connected with the four corresponding rectangular limiting grooves in a sliding mode, and a plurality of uniformly distributed exchanging holes are formed in one side of each rectangular engaging block.
Preferably, a plurality of evenly distributed water bath holes are formed in one end of the limiting plate, a water bath test tube is arranged in the water bath holes, a convenient handball is fixedly connected to the top of the limiting plate, a heating frame is fixedly connected to the inside of the sealing cavity, a heating wire is fixedly connected to the inside of the heating frame, the position of the heating frame corresponds to that of the test tube clamp, a round hole is formed in the bottom of the heating frame, a controller is fixedly connected to one side of the water bath box, and a heating control line is fixedly connected to one side of the controller.
Preferably, one end of the heating control wire penetrates through the round hole and extends into the heating frame to be connected with the heating wire, a driving motor is fixedly connected in the gear cavity, one side of the driving motor is fixedly connected with the driving control wire, the other end of the driving control wire is connected with the controller, the output end of the driving motor is fixedly connected with a coupler, one end of the coupler is fixedly connected with an input shaft, and the top end of the input shaft is fixedly connected with a driving gear.
Preferably, a plurality of evenly distributed rotating holes are formed in one end of the water bath partition plate and one end of the gear partition plate, two corresponding rotating holes in the same position are rotatably sleeved with one driven shaft, two corresponding rotating holes are respectively formed in two ends of the driven shaft, the driven shafts extend to the outer sides of the rotating holes, two ends of the driven shafts are respectively located in the water bath partition plate and the gear cavity, and cross grooves are formed in the top ends of the driven shafts.
Preferably, a plurality of equal fixedly connected with cross stirring leaf in the cross groove, a plurality of the equal fixedly connected with driven gear of bottom of driven shaft, a plurality of driven gear's position all with the position of driving gear is corresponding, and intermeshing, one side fixedly connected with heat insulating board of heating frame, one side of heat insulating board is equipped with the bottom heat preservation sponge, a plurality of wiring grooves and the arc mounting groove of evenly distributed have been seted up to one side inner wall of water bath.
Preferably, a plurality of arc mounting groove respectively with a plurality of the position of winding displacement groove is corresponding, and a plurality of arc mounting groove respectively with a plurality of corresponding winding displacement groove intercommunication each other, a plurality of equal fixedly connected with temperature sensor in the arc mounting groove, a plurality of temperature sensor's one end is all fixedly connected with heat transfer wire, a plurality of heat transfer wire is located a plurality of corresponding winding displacement groove respectively, a plurality of perforation have been seted up on the lateral wall of one side of water bath case, a plurality of perforation position respectively with a plurality of the position of winding displacement groove is corresponding, and a plurality of perforation all with annular seal groove with winding displacement groove is intercommunication each other.
Preferably, one end of each of the plurality of temperature conducting wires penetrates through the corresponding perforation and extends out of one side of each of the plurality of perforation, one end of each of the plurality of temperature conducting wires is connected with the controller, a sealing cover is arranged at the top of the water bath box, a fixing column is fixedly connected to the top of the sealing cover, a warning lamp is fixedly connected to the top of the fixing column, and a warning wire is fixedly connected to one side of the fixing column.
Preferably, the bottom fixedly connected with ring seal of sealed lid, ring seal's position with ring seal's position is corresponding, and looks adaptation, the one end of sealed lid is equipped with the observation window, the top fixedly connected with of sealed lid two arc handles, the other end of warning wire with the controller is connected, the controller with the control line that rises temperature the conducting wire the warning wire the drive control line is electric connection.
(III) beneficial effects
Compared with the prior art, the invention provides the water bath method activity extraction equipment for the placenta stem cell extract, which has the following beneficial effects:
1. this placenta stem cell extract water bath method is carried active device through starting the controller, and the controller makes the heater strip heat through the control line that heaies up, thereby can heat the water in the water bath through the effect of heater strip, and then in passing into the controller with temperature signal through temperature sensor and temperature transmission wire.
2. This placenta stem cell extract water bath method draws active device, through drive control line start-up driving motor, driving motor's output passes through the shaft coupling and drives the input shaft and rotate, and the input shaft then makes the driving gear rotate, thereby the driving gear makes a plurality of driven gears that mesh mutually rotate, and a plurality of driven gears make corresponding driven shaft rotate, thereby the driven shaft makes corresponding cross stirring leaf rotate, thereby through cross stirring leaf rotation can stir the hot water in the water bath for the hot water misce bene avoids the temperature difference great.
3. This placenta stem cell extract water bath method is carried active device detects the temperature through temperature sensor, in detecting the back passes through the temperature wire with the signal transmission controller, the controller then closes driving motor through drive control line, driving motor and then makes the cross stirring leaf stop rotating to can accurate control water bath interior aqueous overall environment temperature.
4. This placenta stem cell extract water bath method draws active device is through upwards mentioning portable handball for portable handball drives test tube anchor clamps, and then makes the rectangle agreeing with the piece and upwards slides in the spacing inslot of rectangle, thereby makes test tube anchor clamps easily take out, and then places the water bath downthehole with the water bath test tube, places test tube anchor clamps in the water bath case again, thereby can replace artifical manual centre gripping water bath test tube to intensify and draw the activity, avoid the emergence of unexpected circumstances such as test tube drops.
5. This placenta stem cell extract water bath method draws active device through the setting of sealed lid, can seal the water bath to be favorable to extracting the activity, through the setting of heat insulating board, prevent that the heating frame temperature from being too high and leading to bottom heat preservation sponge to toast.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic view of a first perspective cutaway perspective of the present invention;
FIG. 3 is a schematic cross-sectional perspective view of a second view of the present invention;
FIG. 4 is a schematic view of a portion of the three-dimensional structure of FIG. 3 according to the present invention;
FIG. 5 is a schematic perspective view of a test tube fixture according to the present invention;
FIG. 6 is a schematic view of a cross-sectional perspective view of another embodiment of the present invention;
FIG. 7 is a schematic top perspective view of the present invention;
fig. 8 is a schematic perspective view of the seal cover of the present invention.
In the figure: 1. a water bath tank; 2. an annular seal groove; 3. an inner layer of heat-preserving sponge; 4. a rectangular limiting block; 5. a water bath partition; 6. rectangular limit grooves; 7. a flow hole; 8. a test tube clamp; 9. a limiting plate; 10. a water bath plate; 11. a water injection hole; 12. rectangular fitting blocks; 13. exchange holes; 14. a water bath test tube; 15. a convenient handball; 16. a heating frame; 17. a heating wire; 18. a controller; 19. a temperature rise control line; 20. a gear partition; 21. a driving motor; 22. an input shaft; 23. a drive gear; 24. a driven shaft; 25. cross stirring blades; 26. a driven gear; 27. a heat insulating plate; 28. a bottom layer of heat-preserving sponge; 29. an arc-shaped mounting groove; 30. a temperature sensor; 31. a temperature transmission wire; 32. sealing cover; 33. fixing the column; 34. a warning light; 35. caution lead; 36. an annular seal ring; 37. an observation window; 38. an arc-shaped handle; 39. driving the control line.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As described in the background art, the prior art has the defects, and in order to solve the technical problems, the application provides a water bath method extraction device and a use method of placenta stem cell extract.
In a typical implementation mode of the application, as shown in fig. 1-8, a placenta stem cell extract water bath method is carried activity equipment and method of use, including water bath 1, annular seal groove 2 has been seted up at the top of water bath 1, be equipped with inlayer insulation sponge 3 in the annular seal groove 2, one side inner wall fixedly connected with four evenly distributed rectangle stopper 4 of water bath 1, one side inner wall fixedly connected with water bath baffle 5 and gear baffle 20 of water bath 1, the position of water bath baffle 5 corresponds with the position of four rectangle stopper 4, and the bottom of four rectangle stopper 4 all is connected with water bath baffle 5, the one end that water bath baffle 5 and gear baffle 20 are close to each other is equipped with same seal chamber, gear baffle 20 is equipped with same gear chamber with the bottom inner wall of water bath 1, rectangle stopper 4 all has been seted up on one side that four rectangle stopper 4 are close to each other rectangle stopper 6, a plurality of evenly distributed circulation holes 7 have all been seted up on one side of four rectangle stopper 4, a plurality of circulation holes 7 communicate each other with four corresponding rectangle stopper 6 respectively, be equipped with anchor clamps 8 in water bath 1, anchor clamps 8 are fixedly connected with water bath 9 and 10 on the water bath board, 10 side water bath board is located at 10 evenly distributed to water bath board is located to the water bath board is 10.
Further, in the above scheme, the outside fixedly connected with of test tube anchor clamps 8 four evenly distributed's rectangle agreements piece 12, the position of four rectangle agreements piece 12 corresponds with the position of four rectangle spacing groove 6 respectively, and looks adaptation, four rectangle agreements piece 12 respectively with four corresponding rectangle spacing groove 6 sliding connection, a plurality of evenly distributed's exchange hole 13 have all been seted up to one side of four rectangle agreements piece 12 for water is convenient for mix in the stirring process through the setting of exchange hole 13.
Further, in the above-mentioned scheme, a plurality of water bath holes of even distribution have been seted up to the one end of limiting plate 9, be equipped with water bath test tube 14 in the water bath hole, the top fixedly connected with convenient handball 15 of limiting plate 9, fixedly connected with heating frame 16 in the sealed chamber, heating wire 17 of fixedly connected with in the heating frame 16, the position of heating frame 16 corresponds with the position of test tube anchor clamps 8, the round hole has been seted up to the bottom of heating frame 16, one side fixedly connected with controller 18 of water bath 1, one side fixedly connected with temperature rise control line 19 of controller 18, through the setting of temperature rise control line 19, the heating wire 17 of being convenient for heats.
Further, in the above scheme, one end of the heating control wire 19 penetrates through the round hole and extends into the heating frame 16 to be connected with the heating wire 17, the driving motor 21 is fixedly connected in the gear cavity, one side of the driving motor 21 is fixedly connected with the driving control wire 39, the other end of the driving control wire 39 is connected with the controller 18, the output end of the driving motor 21 is fixedly connected with the coupler, one end of the coupler is fixedly connected with the input shaft 22, the top end of the input shaft 22 is fixedly connected with the driving gear 23, and the barrel driving control wire 39 is arranged to facilitate starting of the driving motor 21.
Further, in the above scheme, a plurality of evenly distributed rotating holes are formed in one end of the water bath partition board 5 and one end of the gear partition board 20, two rotating holes opposite to each other in the same position are all rotationally sleeved with the same driven shaft 24, two ends of the driven shafts 24 respectively penetrate through the corresponding two rotating holes and extend to the outer sides of the two rotating holes, two ends of the driven shafts 24 are respectively located in the water bath partition board 5 and the gear cavity, cross grooves are formed in the top ends of the driven shafts 24, and the cross stirring blades 25 are convenient to connect through the setting of the cross grooves.
Further, in the above scheme, all fixedly connected with cross stirring leaf 25 in a plurality of cross grooves, the equal fixedly connected with driven gear 26 of bottom of a plurality of driven shafts 24, the position of a plurality of driven gears 26 is all corresponding with the position of driving gear 23, and intermeshing, one side fixedly connected with heat insulating board 27 of heating frame 16, one side of heat insulating board 27 is equipped with bottom heat preservation sponge 28, a plurality of wiring grooves and arc mounting groove 29 of even distribution have been seted up to one side inner wall of water bath 1, through the setting of heat insulating board 27, prevent that heating frame 16 temperature from rising and lead to the roast of bottom heat preservation sponge 28.
Further, in the above-mentioned scheme, a plurality of arc mounting grooves 29 respectively correspond with the position of a plurality of winding displacement groove, and a plurality of arc mounting grooves 29 communicate each other with a plurality of winding displacement grooves that correspond respectively, all fixedly connected with temperature sensor 30 in a plurality of arc mounting grooves 29, a plurality of temperature sensor 30's one end all fixedly connected with temperature transmission wire 31, a plurality of temperature transmission wire 31 are located a plurality of winding displacement grooves that correspond respectively, a plurality of perforation have been seted up on the lateral wall of water bath 1, a plurality of perforation positions correspond with the position of a plurality of winding displacement groove respectively, and a plurality of perforation all communicate each other with annular seal groove 2 and winding displacement groove, through temperature sensor 30's setting, can detect the temperature of water bath.
Further, in the above-mentioned scheme, the one end of a plurality of temperature transfer wires 31 runs through corresponding perforation respectively, and all extends outside one side of a plurality of perforation, and the one end of a plurality of temperature transfer wires 31 all is connected with controller 18, and the top of water bath 1 is equipped with sealed lid 32, and the top fixedly connected with fixed column 33 of sealed lid 32, the top fixedly connected with warning light 34 of fixed column 33, one side fixedly connected with warning wire 35 of fixed column 33.
Further, in the above scheme, the bottom fixedly connected with ring seal 36 of sealed lid 32, ring seal's 36 position corresponds with ring seal groove 2's position, and looks adaptation, and the one end of sealed lid 32 is equipped with viewing window 37, and the top fixedly connected with two arc handles 38 of sealed lid 32, the other end of warning wire 35 is connected with controller 18, and controller 18 and heating control line 19, heat transfer wire 31, warning wire 35, drive control line 39 are electric connection, through the setting of viewing window 37, are convenient for observe the extraction condition in the water bath 1.
When the water heater is used, the controller 18 is started, the heating wire 17 is heated through the heating control wire 19, water in the water bath tank 1 can be heated through the action of the heating wire 17, and then a water temperature signal is transmitted into the controller 18 through the temperature sensor 30 and the temperature transmission wire 31.
The controller 18 starts the driving motor 21 through the driving control line 39, the output end of the driving motor 21 drives the input shaft 22 to rotate through the coupler, the input shaft 22 further enables the driving gear 23 to rotate, the driving gear 23 enables the plurality of meshed driven gears 26 to rotate, the plurality of driven gears 26 enable the corresponding driven shafts 24 to rotate, the driven shafts 24 enable the corresponding cross stirring blades 25 to rotate, hot water in the water bath tank 1 can be stirred through the rotation of the cross stirring blades 25, and the hot water is uniformly mixed, so that large water temperature difference is avoided.
The water temperature is detected by the temperature sensor 30, a signal is transmitted into the controller 18 through the temperature transmission wire 31 after the detection, the controller 18 further closes the driving motor 21 through the driving control wire 39, and the driving motor 21 further enables the cross stirring blade 25 to stop rotating, so that the overall environment temperature of the aqueous solution in the water bath tank 1 can be accurately controlled.
Through upwards mentioning portable handball 15 for portable handball 15 drives test tube anchor clamps 8, and then makes the rectangle agreeing with piece 12 upwards slide in rectangle spacing groove 6, thereby makes test tube anchor clamps 8 easily take out, and then places water bath test tube 14 in the water bath hole, puts into water bath 1 with test tube anchor clamps 8 again, thereby can replace artifical manual centre gripping water bath test tube 14 to intensify and draw the activity, avoid the emergence of unexpected circumstances such as test tube drop.
The water bath 1 can be sealed through the arrangement of the sealing cover 32, so that the activity is extracted, and the heating frame 16 is prevented from being too high in temperature to cause the baking of the bottom heat-insulating sponge through the arrangement of the heat-insulating plate 27.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. The utility model provides a placenta stem cell extract water bath method draws active equipment, includes water bath (1), its characterized in that: annular seal groove (2) has been seted up at the top of water bath (1), be equipped with inlayer heat preservation sponge (3) in annular seal groove (2), four evenly distributed rectangle stopper (4) of one side inner wall fixedly connected with of water bath (1), rectangle spacing groove (6) have all been seted up to one side inner wall fixedly connected with water bath (1) and gear baffle (20), the position of water bath (5) is corresponding with four in the position of rectangle stopper (4), and four rectangle stopper (4) the bottom all with water bath (5) are connected, water bath (5) with one end that gear baffle (20) are close to each other is equipped with same sealed chamber, gear baffle (20) with the bottom inner wall of water bath (1) is equipped with same gear chamber, four rectangle stopper (4) are all seted up rectangle spacing groove (6) are close to each other to one side, four a plurality of evenly distributed circulation hole (7) have all been seted up to one side of rectangle stopper (4), a plurality of corresponding four in the position of rectangle stopper (4) all with water bath (5) are connected, water bath (8) and test tube fixture (8) are equipped with each other in the water bath (8), the limiting plate (9) is positioned above the water bath plate (10), and a plurality of water injection holes (11) which are uniformly distributed are formed in one end of the water bath plate (10);
four uniformly distributed rectangular engaging blocks (12) are fixedly connected to the outer side of the test tube clamp (8), the positions of the four rectangular engaging blocks (12) are respectively corresponding to the positions of the four rectangular limiting grooves (6) and are matched with each other, the four rectangular engaging blocks (12) are respectively corresponding to the positions of the four rectangular limiting grooves (6) in a sliding connection mode, a plurality of uniformly distributed exchanging holes (13) are formed in one side of the four rectangular engaging blocks (12), a plurality of uniformly distributed water bath holes are formed in one end of the limiting plate (9), water bath test tubes (14) are arranged in the water bath holes, a convenient hand ball (15) is fixedly connected to the top of the limiting plate (9), a heating frame (16) is fixedly connected to the inside of the sealing cavity, a heating wire (17) is fixedly connected to the inside of the heating frame (16), round holes are formed in the bottom of the heating frame (16), a controller (18) is fixedly connected to one side of the water bath box (1), a heating wire (19) is fixedly connected to one side of the heating wire (19), one side of the driving motor (21) is fixedly connected with a driving control line (39), the other end of the driving control line (39) is connected with the controller (18), the output end of the driving motor (21) is fixedly connected with a coupler, one end of the coupler is fixedly connected with an input shaft (22), the top end of the input shaft (22) is fixedly connected with a driving gear (23), a plurality of uniformly distributed rotating holes are formed in one end of the water bath partition plate (5) and one end of the gear partition plate (20), two corresponding rotating holes in the same position are rotatably sleeved with the same driven shaft (24), two ends of the driven shafts (24) respectively penetrate through the corresponding two rotating holes and extend to the outer sides of the two rotating holes, two ends of the driven shafts (24) are respectively positioned in the water bath partition plate (5) and the gear cavity, and cross grooves are formed in the top ends of the driven shafts (24);
when the water-bath device is used, the controller (18) is started, the heating wire (17) is heated through the heating control wire (19), water in the water-bath box (1) can be heated through the action of the heating wire (17), and then a water temperature signal is transmitted into the controller (18) through the temperature sensor (30) and the temperature transmission wire (31);
the controller (18) starts the driving motor (21) through the driving control line (39), the output end of the driving motor (21) drives the input shaft (22) to rotate through the coupler, the input shaft (22) further enables the driving gear (23) to rotate, the driving gear (23) enables a plurality of meshed driven gears (26) to rotate, the driven gears (26) enable the corresponding driven shafts (24) to rotate, the driven shafts (24) enable the corresponding cross stirring blades (25) to rotate, hot water in the water bath (1) can be stirred through the rotation of the cross stirring blades (25), and the hot water is uniformly mixed, so that large water temperature differences are avoided; the water temperature is detected through the temperature sensor (30), a signal is transmitted into the controller (18) through the temperature transmission wire (31) after the detection, the controller (18) further closes the driving motor (21) through the driving control wire (39), and the driving motor (21) further enables the cross stirring blade (25) to stop rotating, so that the overall environment temperature of the aqueous solution in the water bath tank (1) can be accurately controlled; by lifting the portable handball (15) upwards, the portable handball (15) drives the test tube clamp (8), and then the rectangular fitting block (12) slides upwards in the rectangular limiting groove (6), so that the test tube clamp (8) is easy to take out, a water bath test tube (14) is placed in a water bath hole, the test tube clamp (8) is placed in the water bath box (1) again, and therefore the manual clamping of the water bath test tube (14) can be replaced for heating extraction activity, and the test tube is prevented from falling off; through the setting of sealed lid (32), can seal water bath (1) to be favorable to extracting the activity, through the setting of heat insulating board (27), prevent that heating frame (16) high temperature from leading to the bottom heat preservation sponge to toast.
2. The apparatus for extracting placental stem cell extract according to claim 1, wherein: a plurality of equal fixedly connected with cross stirring leaf (25) in the cross groove, a plurality of the equal fixedly connected with driven gear (26) of bottom of driven shaft (24), a plurality of the position of driven gear (26) all with the position of driving gear (23) is corresponding, and intermeshing, one side fixedly connected with heat insulating board (27) of heating frame (16), one side of heat insulating board (27) is equipped with bottom heat preservation sponge (28), a plurality of winding displacement grooves and arc mounting groove (29) that distribute evenly have been seted up to one side inner wall of water bath (1).
3. The apparatus for extracting placental stem cell extract according to claim 2, wherein: a plurality of arc mounting groove (29) respectively with a plurality of the position of winding displacement groove is corresponding, and a plurality of arc mounting groove (29) respectively with a plurality of corresponding winding displacement groove intercommunication each other, a plurality of all fixedly connected with temperature sensor (30) in arc mounting groove (29), a plurality of temperature sensor (30)'s one end all fixedly connected with heat transfer wire (31), a plurality of heat transfer wire (31) are located a plurality of corresponding winding displacement groove respectively, a plurality of perforation have been seted up on the lateral wall of one side of water bath (1), a plurality of perforated position respectively with a plurality of the position of winding displacement groove corresponds, and a plurality of perforation all with annular seal groove (2) with winding displacement groove intercommunication each other.
4. The apparatus for extracting placental stem cell extract according to claim 3, wherein: one end of a plurality of heat transfer wire (31) runs through corresponding perforation respectively, and all extends to a plurality of outside one side of perforation, a plurality of heat transfer wire (31) one end all with controller (18) are connected, the top of water bath (1) is equipped with sealed lid (32), the top fixedly connected with fixed column (33) of sealed lid (32), the top fixedly connected with warning light (34) of fixed column (33), one side fixedly connected with warning wire (35) of fixed column (33).
5. The apparatus for extracting placental stem cell extract according to claim 4, wherein: the bottom fixedly connected with ring seal (36) of sealed lid (32), the position of ring seal (36) with the position of ring seal groove (2) is corresponding, and looks adaptation, the one end of sealed lid (32) is equipped with observation window (37), the top fixedly connected with of sealed lid (32) two arc handles (38), the other end of warning wire (35) with controller (18) are connected, controller (18) with intensification control line (19) heat transfer wire (31) warning wire (35) drive control line (39) are electric connection.
CN202110512755.XA 2021-05-11 2021-05-11 Water bath method activity extraction equipment for placenta stem cell extract and use method Active CN113210027B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110512755.XA CN113210027B (en) 2021-05-11 2021-05-11 Water bath method activity extraction equipment for placenta stem cell extract and use method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110512755.XA CN113210027B (en) 2021-05-11 2021-05-11 Water bath method activity extraction equipment for placenta stem cell extract and use method

Publications (2)

Publication Number Publication Date
CN113210027A CN113210027A (en) 2021-08-06
CN113210027B true CN113210027B (en) 2023-06-23

Family

ID=77094740

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110512755.XA Active CN113210027B (en) 2021-05-11 2021-05-11 Water bath method activity extraction equipment for placenta stem cell extract and use method

Country Status (1)

Country Link
CN (1) CN113210027B (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN205288468U (en) * 2015-11-27 2016-06-08 新乡学院 Test tube heating heat preservation device for chemistry experiments

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101184360B1 (en) * 2010-10-01 2012-09-20 한일과학산업 주식회사 Stirring apparatus of thermostat
CN207756168U (en) * 2017-12-29 2018-08-24 江西乐成生物医疗有限公司 A kind of water bath constant temperature heating device
CN211111970U (en) * 2019-11-04 2020-07-28 湖北省生命源干细胞有限公司 Adipose tissue resuscitation device
CN211546563U (en) * 2019-11-28 2020-09-22 昆明医科大学 Primary cell isolated culture device of lung cancer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN205288468U (en) * 2015-11-27 2016-06-08 新乡学院 Test tube heating heat preservation device for chemistry experiments

Also Published As

Publication number Publication date
CN113210027A (en) 2021-08-06

Similar Documents

Publication Publication Date Title
CN109479440B (en) Seed soaking and sterilizing device for agricultural scientific research and breeding
CN113210027B (en) Water bath method activity extraction equipment for placenta stem cell extract and use method
CN112426929A (en) Tea-seed oil production and processing is with concise heating device
CN212254805U (en) Lung cancer screening kit
CN208512412U (en) A kind of laboratory test tube stirring device
CN107446816B (en) Detection device suitable for PCR or HRM detection and analysis
CN215638587U (en) Microbial staining slide drying device
CN213537869U (en) Microorganism constant temperature incubator
CN212299922U (en) Boric acid mixture heating device
CN204988839U (en) Novel automatic dyeing machine
CN212174961U (en) Fluorescent quantitative PCR instrument
CN214300157U (en) Fluorescence detector convenient to use
CN220437934U (en) Reinforced tissue dyeing machine
CN215437824U (en) A sample storage cabinet for honey quality testing usefulness
CN219141198U (en) Internal circulation ventilation medicine refrigerated cabinet
CN214916235U (en) High-temperature constant-temperature circulating tank capable of rapidly heating
CN218654468U (en) Microbial fertilizer accelerated reaction device
CN215866702U (en) But water-bath heating's escherichia coli detect reagent box
CN210834888U (en) Environmental monitoring water quality collection device
CN217297806U (en) Drug fermentation device for biosynthesis
CN216651166U (en) High-temperature sterilization device for extracting pig bone concentrated solution
CN215328017U (en) Integrated digital PCR system
CN215708275U (en) Animal doctor is with animal blood sampling heat preservation device
CN219719286U (en) Soil sterilization device
CN214898325U (en) Temperature-controllable scanning electron microscope sample stage

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20230530

Address after: 610000 China (Sichuan) pilot Free Trade Zone, Chengdu, Sichuan, No. 101, 204, 302, 1 / F, building 1, No. 88, jiaozi North 1st Road, high tech Zone, Chengdu

Applicant after: SICHUAN WUYAN BIOTECHNOLOGY Co.,Ltd.

Address before: 510000 Room 101, No. 21, Fengchi Road, Hailong street, Liwan District, Guangzhou City, Guangdong Province

Applicant before: Song Zhengbao

TA01 Transfer of patent application right
GR01 Patent grant
GR01 Patent grant