CN113208011A - Laying hen feed for improving egg quality - Google Patents
Laying hen feed for improving egg quality Download PDFInfo
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- CN113208011A CN113208011A CN202110653455.3A CN202110653455A CN113208011A CN 113208011 A CN113208011 A CN 113208011A CN 202110653455 A CN202110653455 A CN 202110653455A CN 113208011 A CN113208011 A CN 113208011A
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- cellulase
- green coffee
- citric acid
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- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 58
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 58
- 241000252230 Ctenopharyngodon idella Species 0.000 claims abstract description 29
- 239000004471 Glycine Substances 0.000 claims abstract description 29
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims abstract description 29
- 235000003704 aspartic acid Nutrition 0.000 claims abstract description 29
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 235000013353 coffee beverage Nutrition 0.000 claims abstract description 29
- 235000013601 eggs Nutrition 0.000 claims abstract description 29
- 239000011780 sodium chloride Substances 0.000 claims abstract description 29
- 244000303040 Glycyrrhiza glabra Species 0.000 claims abstract description 22
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims abstract description 22
- 240000008042 Zea mays Species 0.000 claims abstract description 22
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 22
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 22
- 229940036811 bone meal Drugs 0.000 claims abstract description 22
- 239000002374 bone meal Substances 0.000 claims abstract description 22
- 235000005822 corn Nutrition 0.000 claims abstract description 22
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims abstract description 22
- 235000011477 liquorice Nutrition 0.000 claims abstract description 22
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 22
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000011575 calcium Substances 0.000 claims abstract description 14
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 14
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 126
- 108010059892 Cellulase Proteins 0.000 claims description 51
- 229940106157 cellulase Drugs 0.000 claims description 51
- 239000000706 filtrate Substances 0.000 claims description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 40
- 239000007864 aqueous solution Substances 0.000 claims description 33
- 238000002156 mixing Methods 0.000 claims description 33
- 239000008367 deionised water Substances 0.000 claims description 29
- 229910021641 deionized water Inorganic materials 0.000 claims description 29
- 241000533293 Sesbania emerus Species 0.000 claims description 21
- 238000002791 soaking Methods 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 19
- 238000001816 cooling Methods 0.000 claims description 18
- 238000001914 filtration Methods 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 16
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims description 15
- 235000019700 dicalcium phosphate Nutrition 0.000 claims description 15
- 241001183967 Isodon Species 0.000 claims description 14
- 244000025254 Cannabis sativa Species 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 241000743774 Brachypodium Species 0.000 claims description 8
- 244000115901 Petasites palmatus Species 0.000 claims description 8
- 235000006789 Petasites palmatus Nutrition 0.000 claims description 8
- 235000002639 sodium chloride Nutrition 0.000 claims description 8
- 235000019764 Soybean Meal Nutrition 0.000 claims description 6
- 239000004455 soybean meal Substances 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 3
- 241000608170 Gymnadenia conopsea Species 0.000 claims description 2
- 241001113309 Petasites tricholobus Species 0.000 claims 1
- 244000046052 Phaseolus vulgaris Species 0.000 abstract description 23
- 235000010627 Phaseolus vulgaris Nutrition 0.000 abstract description 23
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 10
- 235000012000 cholesterol Nutrition 0.000 abstract description 5
- 210000002969 egg yolk Anatomy 0.000 abstract description 5
- 150000003904 phospholipids Chemical class 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 241000287828 Gallus gallus Species 0.000 description 16
- 235000013330 chicken meat Nutrition 0.000 description 16
- 238000003756 stirring Methods 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 14
- 239000011259 mixed solution Substances 0.000 description 14
- 235000015097 nutrients Nutrition 0.000 description 9
- 210000000988 bone and bone Anatomy 0.000 description 7
- 238000010438 heat treatment Methods 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 210000000582 semen Anatomy 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 241000382298 Lagotis Species 0.000 description 6
- 241000800405 Spatholobus suberectus Species 0.000 description 6
- 229940069765 bean extract Drugs 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 229940023569 palmate Drugs 0.000 description 4
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 241000243695 Brachyporus Species 0.000 description 1
- 241000132542 Conyza Species 0.000 description 1
- 241000191398 Kalanchoe blossfeldiana Species 0.000 description 1
- 240000006915 Petasites Species 0.000 description 1
- 240000003296 Petasites japonicus Species 0.000 description 1
- 235000003823 Petasites japonicus Nutrition 0.000 description 1
- 241000913745 Spatholobus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000020299 breve Nutrition 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000010908 plant waste Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Birds (AREA)
- Inorganic Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a laying hen feed for improving egg quality, which comprises corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrophosphate, sodium chloride, glycine, aspartic acid, liquorice and green coffee bean-lagochilus brachypomus-petasites tricholobus extract. The laying hen feed prepared by the invention can obviously reduce the cholesterol content in eggs laid by laying hens, obviously improve the yolk phospholipid content, improve the quality of eggs, and obtain the eggs with higher nutritional value.
Description
Technical Field
The invention relates to the technical field of feed components, and particularly relates to a laying hen feed for improving egg quality.
Background
The chicken feed is a nutrient material for feeding chickens, which is prepared from various crop wastes according to a certain proportion and is fully and uniformly stirred. The chicken feed is a carrier of nutrient substances required by the chickens in the growth process, and the nutrient substances (including carbohydrates, fat, proteins, minerals, vitamins and other trace elements) required by the chickens are provided by the feed. The feed can not only provide nutrient substances required for the growth of the chickens, but also provide the resistance of the chickens and enhance the disease resistance of the chickens. Generally, in order to ensure that chickens can thrive better, various nutrient substances are additionally added into the feed in the production process, some nutrient substances are used for supplementing nutrient elements to the chickens, some nutrient substances are used for enhancing the resistance and disease resistance of the chickens, and some nutrient substances are used for improving the quality of eggs.
Disclosure of Invention
The invention provides a laying hen feed for improving egg quality, which comprises corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrophosphate, sodium chloride, glycine, aspartic acid, liquorice and green coffee bean-lagochilus brachypomus-petasites japonicus extract.
Further, the preparation method of the green coffee bean-conyza brachypodioides-petasites japonicus extract comprises the following steps:
(1) mixing green coffee bean, entire plant of Rabdosia brachyota (Hemsl.) Hara, and leaf of Petasites palmatus (Hemsl.) Hara, chopping, adding deionized water into the chopped mixture, extracting in water bath at constant temperature of 85 + -5 deg.C, air cooling to normal temperature, and filtering to obtain filtrate A and residue;
(2) preparing a mixed aqueous solution of citric acid and cellulase, soaking the filter residue in the mixed aqueous solution of citric acid and cellulase, soaking and extracting at 55 +/-5 ℃, cooling to normal temperature after extraction is finished, filtering to obtain filtrate B, mixing the filtrate A with the filtrate B, and concentrating under reduced pressure to obtain the green coffee bean-rabdosia brachyporus-petiolus japonicus extract.
Further, the green coffee beans, the whole grass of the rabdosia brachypodium and the spatholobus leaf are mixed with each other in a mixing mass ratio of green coffee beans: all grass of Rabdosia brachypodium: the leaf of the gymnadenia conopsea is 3: 6-9: 12-14.
Further, in the step (1), adding deionized water into the mixture after chopping, wherein the mixing mass ratio of the mixture to the deionized water is 1: 7-10; and (3) carrying out water bath at constant temperature of 85 +/-5 ℃ for extracting for 80-100 min.
Further, the preparation method of the citric acid and cellulase mixed aqueous solution comprises the following steps: firstly, adding cellulase into deionized water to prepare a solution with the mass percentage of the cellulase being 0.5-0.8%, and then adding citric acid to adjust the pH value of the solution to 4.8-5.0, thus obtaining the citric acid and cellulase mixed aqueous solution.
Further, in the step (2), the filter residue is soaked in the mixed aqueous solution of citric acid and cellulase, wherein the solid-liquid mass ratio of the filter residue to the mixed aqueous solution of citric acid and cellulase is 1: 6-8; soaking and extracting for 5-6 h at 55 +/-5 ℃.
Further, the mixture of filtrate A and filtrate B was concentrated under reduced pressure to 1/4 in the volume before concentration.
Further, the laying hen feed comprises the following components in parts by weight: the feed comprises 50 parts of corn, 6-10 parts of wheat bran, 16-20 parts of soybean meal, 5-8 parts of grass carp bone meal, 2-3 parts of calcium hydrophosphate, 0.8-1.5 parts of sodium chloride, 0.08-0.1 part of glycine, 0.08-0.1 part of aspartic acid, 2-5 parts of liquorice and 8-11 parts of green coffee bean-short-spike lagotis-petasites tricholobus extract.
The invention has the beneficial effects that: the laying hen feed prepared by the invention can obviously reduce the cholesterol content in eggs laid by laying hens, obviously improve the yolk phospholipid content, improve the quality of eggs, and obtain the eggs with higher nutritional value.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
A laying hen feed for improving egg quality comprises semen Maydis, testa Tritici, bean cake, grass carp bone powder, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid, Glycyrrhrizae radix and green coffee bean-herba Lespedezae Bicoloris-rhizoma Petasitis Japonici extract. The components are respectively as follows according to parts by weight: comprises 50 parts of corn, 6 parts of wheat bran, 16 parts of bean pulp, 5 parts of grass carp bone meal, 2 parts of calcium hydrophosphate, 0.8 part of sodium chloride, 0.08 part of glycine, 0.08 part of aspartic acid, 2 parts of liquorice and 8 parts of green coffee bean-lagotis brachypomus-petasites japonicus extract.
The preparation method of the green coffee bean-conyza brachypodioides-petasites palmatum extract comprises the following steps:
(1) taking green coffee beans, the whole grass of the rabdosia brachypoides and the leaf of the spatholobus suberectus dunn as the green coffee beans according to the mass ratio: all grass of Rabdosia brachypodium: mixing and chopping the petasites palmatus leaves in a ratio of 3:6:12, adding deionized water into the chopped mixture, wherein the mixing mass ratio of the mixture to the deionized water is 1: 7; heating in water bath at constant temperature of 85 + -5 deg.C, extracting for 80min, air cooling to room temperature, and filtering to obtain filtrate A and residue;
(2) and adding cellulase into the deionized water to prepare a solution with the mass percentage of the cellulase being 0.5%, and adding citric acid to adjust the pH of the solution to 4.8 to obtain a citric acid and cellulase mixed aqueous solution. Soaking the filter residue in the mixed aqueous solution of citric acid and cellulase, wherein the solid-liquid mass ratio of the filter residue to the mixed aqueous solution of citric acid and cellulase is 1: 6; soaking and extracting at 55 + -5 deg.C for 5h, air cooling to room temperature after extraction, filtering to obtain filtrate B, mixing filtrate A and filtrate B to form a mixed solution, and concentrating the mixed solution under reduced pressure to 1/4 of the volume before concentration to obtain the green coffee bean-Lagotis brachypodia-Palmata extract.
Preparing feed within 2 hours before feeding, mixing and stirring corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid and liquorice uniformly, then scattering green coffee bean-lagotis livida-petasites palmate dudou extract, and stirring uniformly.
Example 2
A laying hen feed for improving egg quality comprises semen Maydis, testa Tritici, bean cake, grass carp bone powder, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid, Glycyrrhrizae radix and green coffee bean-herba Lespedezae Bicoloris-rhizoma Petasitis Japonici extract. The components are respectively as follows according to parts by weight: comprises 50 parts of corn, 7 parts of wheat bran, 17 parts of bean pulp, 6 parts of grass carp bone meal, 2 parts of calcium hydrophosphate, 1.1 parts of sodium chloride, 0.09 part of glycine, 0.09 part of aspartic acid, 3 parts of liquorice and 9 parts of green coffee bean-lagotis brachypomus-petasites japonicus extract.
The preparation method of the green coffee bean-conyza brachypodioides-petasites palmatum extract comprises the following steps:
(1) taking green coffee beans, the whole grass of the rabdosia brachypoides and the leaf of the spatholobus suberectus dunn as the green coffee beans according to the mass ratio: all grass of Rabdosia brachypodium: mixing and chopping the petasites palmatus leaves in a ratio of 3:7:13, adding deionized water into the chopped mixture, wherein the mixing mass ratio of the mixture to the deionized water is 1: 7; heating in water bath at constant temperature of 85 + -5 deg.C, extracting for 90min, air cooling to room temperature, and filtering to obtain filtrate A and residue;
(2) and adding cellulase into the deionized water to prepare a solution with the mass percentage of the cellulase being 0.6%, and adding citric acid to adjust the pH of the solution to 4.9 to obtain a citric acid and cellulase mixed aqueous solution. Soaking the filter residue in the mixed aqueous solution of citric acid and cellulase, wherein the solid-liquid mass ratio of the filter residue to the mixed aqueous solution of citric acid and cellulase is 1: 6; soaking and extracting at 55 + -5 deg.C for 5h, air cooling to room temperature after extraction, filtering to obtain filtrate B, mixing filtrate A and filtrate B to form a mixed solution, and concentrating the mixed solution under reduced pressure to 1/4 of the volume before concentration to obtain the green coffee bean-Lagotis brachypodia-Palmata extract.
Preparing feed within 2 hours before feeding, mixing and stirring corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid and liquorice uniformly, then scattering green coffee bean-lagotis livida-petasites palmate dudou extract, and stirring uniformly.
Example 3
A laying hen feed for improving egg quality comprises semen Maydis, testa Tritici, bean cake, grass carp bone powder, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid, Glycyrrhrizae radix and green coffee bean-herba Lespedezae Bicoloris-rhizoma Petasitis Japonici extract. The components are respectively as follows according to parts by weight: comprises 50 parts of corn, 8 parts of wheat bran, 18 parts of bean pulp, 7 parts of grass carp bone meal, 3 parts of calcium hydrophosphate, 1.3 parts of sodium chloride, 0.09 part of glycine, 0.09 part of aspartic acid, 4 parts of liquorice and 10 parts of green coffee bean-lagotis brachypomus-petasites japonicus extract.
The preparation method of the green coffee bean-conyza brachypodioides-petasites palmatum extract comprises the following steps:
(1) taking green coffee beans, the whole grass of the rabdosia brachypoides and the leaf of the spatholobus suberectus dunn as the green coffee beans according to the mass ratio: all grass of Rabdosia brachypodium: mixing and chopping petasites palmatus leaves in a ratio of 3:8:13, adding deionized water into the chopped mixture, wherein the mixing mass ratio of the mixture to the deionized water is 1: 7; heating in water bath at constant temperature of 85 + -5 deg.C, extracting for 90min, air cooling to room temperature, and filtering to obtain filtrate A and residue;
(2) and adding cellulase into the deionized water to prepare a solution with the mass percentage of the cellulase being 0.7%, and adding citric acid to adjust the pH of the solution to 4.9 to obtain a citric acid and cellulase mixed aqueous solution. Soaking the filter residue in the mixed aqueous solution of citric acid and cellulase, wherein the solid-liquid mass ratio of the filter residue to the mixed aqueous solution of citric acid and cellulase is 1: 6; soaking and extracting at 55 + -5 deg.C for 6h, air cooling to room temperature after extraction, filtering to obtain filtrate B, mixing filtrate A and filtrate B to form a mixed solution, and concentrating the mixed solution under reduced pressure to 1/4 of the volume before concentration to obtain the green coffee bean-Lagotis brachypodia-Palmata extract.
Preparing feed within 2 hours before feeding, mixing and stirring corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid and liquorice uniformly, then scattering green coffee bean-lagotis livida-petasites palmate dudou extract, and stirring uniformly.
Example 4
A laying hen feed for improving egg quality comprises semen Maydis, testa Tritici, bean cake, grass carp bone powder, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid, Glycyrrhrizae radix and green coffee bean-herba Lespedezae Bicoloris-rhizoma Petasitis Japonici extract. The components are respectively as follows according to parts by weight: comprises 50 parts of corn, 10 parts of wheat bran, 20 parts of bean pulp, 8 parts of grass carp bone meal, 3 parts of calcium hydrophosphate, 1.5 parts of sodium chloride, 0.1 part of glycine, 0.1 part of aspartic acid, 5 parts of liquorice and 11 parts of green coffee bean-lagotis brachypomus-petasites japonicus extract.
The preparation method of the green coffee bean-conyza brachypodioides-petasites palmatum extract comprises the following steps:
(1) taking green coffee beans, the whole grass of the rabdosia brachypoides and the leaf of the spatholobus suberectus dunn as the green coffee beans according to the mass ratio: all grass of Rabdosia brachypodium: mixing and chopping the petasites palmatus leaves in a ratio of 3:9:14, adding deionized water into the chopped mixture, wherein the mixing mass ratio of the mixture to the deionized water is 1: 7; heating in water bath at constant temperature of 85 + -5 deg.C, extracting for 100min, air cooling to room temperature, and filtering to obtain filtrate A and residue;
(2) and adding cellulase into the deionized water to prepare a solution with the mass percentage of the cellulase being 0.8%, and adding citric acid to adjust the pH of the solution to 5.0 to obtain a citric acid and cellulase mixed aqueous solution. Soaking the filter residue in the mixed aqueous solution of citric acid and cellulase, wherein the solid-liquid mass ratio of the filter residue to the mixed aqueous solution of citric acid and cellulase is 1: 6; soaking and extracting at 55 + -5 deg.C for 6h, air cooling to room temperature after extraction, filtering to obtain filtrate B, mixing filtrate A and filtrate B to form a mixed solution, and concentrating the mixed solution under reduced pressure to 1/4 of the volume before concentration to obtain the green coffee bean-Lagotis brachypodia-Palmata extract.
Preparing feed within 2 hours before feeding, mixing and stirring corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid and liquorice uniformly, then scattering green coffee bean-lagotis livida-petasites palmate dudou extract, and stirring uniformly.
Comparative example 1
A laying hen feed comprises semen Maydis, testa Tritici, bean cake, grass carp bone powder, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid, Glycyrrhrizae radix and green coffee bean extract. The components are respectively as follows according to parts by weight: comprises 50 parts of corn, 7 parts of wheat bran, 17 parts of bean pulp, 6 parts of grass carp bone meal, 2 parts of calcium hydrophosphate, 1.1 parts of sodium chloride, 0.09 part of glycine, 0.09 part of aspartic acid, 3 parts of liquorice and 9 parts of green coffee bean extract.
The preparation method of the green coffee bean extract comprises the following steps:
(1) crushing green coffee beans, adding deionized water into the crushed material, wherein the mixing mass ratio of the crushed material to the deionized water is 1: 7; heating in water bath at constant temperature of 85 + -5 deg.C, extracting for 90min, air cooling to room temperature, and filtering to obtain filtrate A and residue;
(2) and adding cellulase into the deionized water to prepare a solution with the mass percentage of the cellulase being 0.6%, and adding citric acid to adjust the pH of the solution to 4.9 to obtain a citric acid and cellulase mixed aqueous solution. Soaking the filter residue in the mixed aqueous solution of citric acid and cellulase, wherein the solid-liquid mass ratio of the filter residue to the mixed aqueous solution of citric acid and cellulase is 1: 6; soaking and extracting at 55 +/-5 ℃ for 5 hours, cooling to normal temperature after extraction is finished, filtering to obtain filtrate B, mixing the filtrate A and the filtrate B to form a mixed solution, and concentrating the mixed solution under reduced pressure to 1/4 of the volume before concentration to obtain the green coffee bean extract of the comparative example.
Preparing feed within 2 hours before feeding, mixing and stirring corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrophosphate, sodium chloride, glycine, aspartic acid and liquorice uniformly, then scattering the green coffee bean extract, and stirring uniformly.
Comparative example 2
A feed for laying hens comprises semen Maydis, testa Tritici, bean cake, grass carp bone powder, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid, Glycyrrhrizae radix and herba Lagotis extract. The components are respectively as follows according to parts by weight: comprises 50 parts of corn, 7 parts of wheat bran, 17 parts of soybean meal, 6 parts of grass carp bone meal, 2 parts of calcium hydrophosphate, 1.1 parts of sodium chloride, 0.09 part of glycine, 0.09 part of aspartic acid, 3 parts of liquorice and 9 parts of a lagotis brachypomus extract.
The preparation method of the lagotis brachypodium extract comprises the following steps:
(1) cutting whole grass of the lagotis breve, adding deionized water into the cut material, wherein the mass ratio of the cut material to the deionized water is 1: 7; heating in water bath at constant temperature of 85 + -5 deg.C, extracting for 90min, air cooling to room temperature, and filtering to obtain filtrate A and residue;
(2) and adding cellulase into the deionized water to prepare a solution with the mass percentage of the cellulase being 0.6%, and adding citric acid to adjust the pH of the solution to 4.9 to obtain a citric acid and cellulase mixed aqueous solution. Soaking the filter residue in the mixed aqueous solution of citric acid and cellulase, wherein the solid-liquid mass ratio of the filter residue to the mixed aqueous solution of citric acid and cellulase is 1: 6; soaking and extracting at 55 +/-5 ℃ for 5 hours, cooling to normal temperature after extraction is finished, filtering to obtain filtrate B, mixing the filtrate A and the filtrate B to form a mixed solution, and concentrating the mixed solution under reduced pressure to 1/4 of the volume before concentration to obtain the lagotis brachyporus extract of the comparative example.
Preparing feed within 2 hours before feeding, mixing and stirring corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid and liquorice uniformly, then scattering the lagotis brachypomus extract, and stirring uniformly.
Comparative example 3
A laying hen feed comprises semen Maydis, testa Tritici, bean cake, grass carp bone powder, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid, Glycyrrhrizae radix and petasites japonicus extract. The components are respectively as follows according to parts by weight: comprises 50 parts of corn, 7 parts of wheat bran, 17 parts of bean pulp, 6 parts of grass carp bone meal, 2 parts of calcium hydrophosphate, 1.1 parts of sodium chloride, 0.09 part of glycine, 0.09 part of aspartic acid, 3 parts of liquorice and 9 parts of gymnasium conopsea extract.
The preparation method of the petasites palmatus extract comprises the following steps:
(1) chopping the spatholobus suberectus hance leaves, adding deionized water into the chopped matter, wherein the mixing mass ratio of the chopped matter to the deionized water is 1: 7; heating in water bath at constant temperature of 85 + -5 deg.C, extracting for 90min, air cooling to room temperature, and filtering to obtain filtrate A and residue;
(2) and adding cellulase into the deionized water to prepare a solution with the mass percentage of the cellulase being 0.6%, and adding citric acid to adjust the pH of the solution to 4.9 to obtain a citric acid and cellulase mixed aqueous solution. Soaking the filter residue in the mixed aqueous solution of citric acid and cellulase, wherein the solid-liquid mass ratio of the filter residue to the mixed aqueous solution of citric acid and cellulase is 1: 6; soaking and extracting at 55 +/-5 ℃ for 5 hours, cooling to normal temperature after extraction is finished, filtering to obtain filtrate B, mixing the filtrate A and the filtrate B to form a mixed solution, and concentrating the mixed solution under reduced pressure to 1/4 of the volume before concentration to obtain the petasites palmatus extract of the comparative example.
Preparing feed within 2 hours before feeding, mixing and stirring corn, wheat bran, soybean meal, grass carp bone meal, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid and liquorice uniformly, then spreading the spatholobus suberectus extract, and stirring uniformly.
Comparative example 4
A laying hen feed comprises corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid and liquorice. The components are respectively as follows according to parts by weight: comprises 50 parts of corn, 7 parts of wheat bran, 17 parts of soybean meal, 6 parts of grass carp bone meal, 2 parts of calcium hydrophosphate, 1.1 parts of sodium chloride, 0.09 part of glycine, 0.09 part of aspartic acid and 3 parts of liquorice. Preparing feed within 2 hours before feeding, and mixing and stirring corn, wheat bran, bean pulp, grass carp bone meal, calcium hydrophosphate, sodium chloride, glycine, aspartic acid and liquorice uniformly during preparation.
Example 5
The Hailan brown shell laying hens are used as research objects. 480 healthy 35-week-old kalanchoe brown-shell laying hens with similar weight are selected in the same henhouse and divided into eight groups, wherein each group comprises 60 hens, each group comprises three groups, and each group comprises 20 hens. The test chickens were raised in 3-tier chicken cages arranged in a stepwise manner, and 5 chickens were raised in 1 cage. The inside of the chicken house is illuminated by an incandescent lamp for 12 hours every day, the chicken house is ventilated longitudinally, free feeding and drinking are carried out, and feed is fed for three times every day at 7: 30-8: 00, 12: 00-12: 30 and 17: 30-18: 00. The same group is fed with the same feed, and each group in the same group is independently managed and fed by different feeders. Different large groups are respectively fed with the layer feed of the above examples 1-4 and the comparative examples 1-4. The daily feeding amount of each cage is 600 g. The day of starting feeding the example or comparative example feed was the first day, and the eggs laid on the 14 th day in each cage were taken and tested for the contents of cholesterol and yolk phospholipids in the eggs, and the average value of each group in the same large group was taken as the result value of the large group, as shown in Table 1.
As can be seen from Table 1, the laying hen feed prepared by the invention can obviously reduce the cholesterol content in eggs laid by laying hens, obviously improve the content of yolk phospholipids, improve the quality of eggs and obtain the eggs with higher nutritional value. Comparing example 2 with each of the comparative examples, it is clear that the effect of the green coffee bean, the conyza brachypodioides and the petasites palmata composite extract is obviously better than that of the single component under the condition of equal addition, and the three effects are the interactive results for improving the quality of the eggs.
TABLE 1
Kinds of feed to be fed | Average Cholesterol content (mg/g) | Percentage of yolk phospholipids by mass (%) |
Example 1 | 6.96±1.39 | 15.23±0.24 |
Example 2 | 6.42±1.22 | 15.80±0.36 |
Example 3 | 6.39±1.68 | 15.94±0.37 |
Example 4 | 6.50±1.34 | 15.66±0.29 |
Comparative example 1 | 14.63±1.90 | 9.84±0.60 |
Comparative example 2 | 11.55±2.01 | 10.82±0.41 |
Comparative example 3 | 10.94±1.47 | 11.07±0.36 |
Comparative example 4 | 16.95±2.64 | 8.85±0.55 |
The technical solutions provided by the present invention are described in detail above, and for those skilled in the art, the ideas according to the embodiments of the present invention may be changed in the specific implementation manners and the application ranges, and in summary, the content of the present description should not be construed as limiting the present invention.
Claims (8)
1. A layer feed for improving egg quality is characterized by comprising corn, wheat bran, soybean meal, grass carp bone meal, calcium hydrogen phosphate, sodium chloride, glycine, aspartic acid, liquorice and green coffee bean-lagochilus brachypomus-petasites japonicus extract.
2. The laying hen feed for improving egg quality according to claim 1, wherein the green coffee bean-conyza brachypodioides-petasites palmatus extract is prepared by the following steps:
(1) mixing green coffee bean, entire plant of Rabdosia brachyota (Hemsl.) Hara, and leaf of Petasites palmatus (Hemsl.) Hara, chopping, adding deionized water into the chopped mixture, extracting in water bath at constant temperature of 85 + -5 deg.C, air cooling to normal temperature, and filtering to obtain filtrate A and residue;
(2) preparing a mixed aqueous solution of citric acid and cellulase, soaking the filter residue in the mixed aqueous solution of citric acid and cellulase, soaking and extracting at 55 +/-5 ℃, cooling to normal temperature after extraction is finished, filtering to obtain filtrate B, mixing the filtrate A with the filtrate B, and concentrating under reduced pressure to obtain the green coffee bean-rabdosia brachyporus-petiolus japonicus extract.
3. The laying hen feed for improving egg quality according to claim 2, wherein the green coffee beans, the whole plant of the rabdosia brachypodi and the petasites tricholobus leaves are mixed with each other in a mixing ratio by mass of green coffee beans: all grass of Rabdosia brachypodium: the leaf of the gymnadenia conopsea is 3: 6-9: 12-14.
4. The layer feed for improving the quality of eggs according to claim 2, wherein in the step (1), deionized water is added to the mixture after chopping, wherein the mixing mass ratio of the mixture to the deionized water is 1: 7-10; and (3) carrying out water bath at constant temperature of 85 +/-5 ℃ for extracting for 80-100 min.
5. The laying hen feed for improving the quality of eggs according to claim 2, wherein the preparation method of the citric acid and cellulase mixed aqueous solution comprises the following steps: firstly, adding cellulase into deionized water to prepare a solution with the mass percentage of the cellulase being 0.5-0.8%, and then adding citric acid to adjust the pH value of the solution to 4.8-5.0, thus obtaining the citric acid and cellulase mixed aqueous solution.
6. The layer feed for improving the quality of eggs according to claim 2, wherein in the step (2), the filter residue is soaked in the mixed aqueous solution of citric acid and cellulase, wherein the solid-liquid mass ratio of the filter residue to the mixed aqueous solution of citric acid and cellulase is 1: 6-8; soaking and extracting for 5-6 h at 55 +/-5 ℃.
7. The laying hen feed for improving egg quality as claimed in claim 2, wherein the mixture of filtrate A and filtrate B is concentrated under reduced pressure to 1/4 in volume before concentration.
8. The layer feed for improving the quality of eggs according to claim 2, wherein the layer feed comprises the following components in parts by weight: the feed comprises 50 parts of corn, 6-10 parts of wheat bran, 16-20 parts of soybean meal, 5-8 parts of grass carp bone meal, 2-3 parts of calcium hydrophosphate, 0.8-1.5 parts of sodium chloride, 0.08-0.1 part of glycine, 0.08-0.1 part of aspartic acid, 2-5 parts of liquorice and 8-11 parts of green coffee bean-short-spike lagotis-petasites tricholobus extract.
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CN102987125A (en) * | 2012-10-10 | 2013-03-27 | 合肥永生禽业有限责任公司 | Petasites japonicus broiler feed |
CN105981982A (en) * | 2016-03-07 | 2016-10-05 | 安徽夏星饲料有限公司 | Laying henfodder capable of improving egg quality and preparation method thereof |
CN106819379A (en) * | 2016-12-31 | 2017-06-13 | 枞阳县恒祥生态农业有限公司 | A kind of 3 ~ 21 ages in days raise chicken feed scattered |
CN107616351A (en) * | 2017-10-26 | 2018-01-23 | 江西祥澜科技发展有限公司 | A kind of feed and feeding method of laying chicken for improving egg quality |
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CN102987125A (en) * | 2012-10-10 | 2013-03-27 | 合肥永生禽业有限责任公司 | Petasites japonicus broiler feed |
CN105981982A (en) * | 2016-03-07 | 2016-10-05 | 安徽夏星饲料有限公司 | Laying henfodder capable of improving egg quality and preparation method thereof |
CN106819379A (en) * | 2016-12-31 | 2017-06-13 | 枞阳县恒祥生态农业有限公司 | A kind of 3 ~ 21 ages in days raise chicken feed scattered |
CN107616351A (en) * | 2017-10-26 | 2018-01-23 | 江西祥澜科技发展有限公司 | A kind of feed and feeding method of laying chicken for improving egg quality |
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