CN113207556A - Simplified edible fungus inoculation device and inoculation method - Google Patents
Simplified edible fungus inoculation device and inoculation method Download PDFInfo
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- CN113207556A CN113207556A CN202110535304.8A CN202110535304A CN113207556A CN 113207556 A CN113207556 A CN 113207556A CN 202110535304 A CN202110535304 A CN 202110535304A CN 113207556 A CN113207556 A CN 113207556A
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- inoculation
- body ring
- shaft disc
- main body
- connecting rod
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
Abstract
The invention discloses a simplified edible fungus inoculation device and an inoculation method, the device comprises an inoculation frame, a base and a support fixedly arranged on the base, the inoculation frame comprises a connecting rod, a supporting rod, a shaft disc, a main body ring and a test tube sleeve fixedly arranged on the main body ring, the shaft disc and the main body ring are connected through a plurality of supporting rods uniformly arranged between the shaft disc and the main body ring, a shaft disc rotating hole is formed in the shaft center of the shaft disc, one end of the connecting rod is rotatably connected with the shaft disc through a connecting piece, and the other end of the connecting rod is connected with the upper end of the support. The device can effectively simplify the inoculation operation and achieve the effect of quick inoculation; the inoculation efficiency is improved, the opportunity of the infection of the slant culture medium is reduced, and the required equipment is simple, the operation is simple, the operability is strong, and the method is suitable for wide popularization and use.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a simplified edible fungus inoculation device and an inoculation method.
Background
Currently, in the inoculation process of edible fungus mother seeds, a mother seed test tube and a slant culture medium to be inoculated are taken up in parallel by a left hand, the slant faces upwards, and a tube opening is placed in a sterile area formed by alcohol lamp flame; the little finger, ring finger and palm of the right hand take down the inclined plane test tube tampon, the test tube mouth is slightly inclined downwards, and the alcohol burner flame is used for sealing the tube mouth; holding the inoculating hook with thumb, index finger and middle finger of right hand, taking a colony of 2-3mm size on the mother seed slant, and rapidly transferring into a new test tube slant; taking out the inoculation hook, plugging a cotton plug, burning a test tube opening and the inoculation hook by using an alcohol lamp for sterilization, and carrying out next inoculation. The matching degree of the hands and the disinfection problem with the alcohol lamp are the problems needing attention in inoculation, the existing inoculation technology has high requirement on the inoculation proficiency of an operator, the process is easy to contaminate bacteria, time and labor are wasted, and the inoculation efficiency is low.
Aiming at the technical problem that the edible fungus inoculation process in the prior art is complex in operation and easy to contaminate, a simplified edible fungus inoculation device needs to be designed, the edible fungus batch inoculation is carried out by adopting the device, the inoculation operation is simplified, the inoculation efficiency is improved, and the risk of contamination of a slant culture medium is reduced.
Disclosure of Invention
The invention aims to provide a simplified edible fungus inoculation device and an inoculation method aiming at the technical problems of the background technology.
The purpose of the invention can be realized by the following technical scheme:
the utility model provides a simple and easy domestic fungus inoculation device, the device is in including inoculation frame, base and fixed setting support on the base, the inoculation frame include connecting rod, bracing piece, reel, main part circle and fixed setting test tube cover that the main part circled, reel and main part circle be in through many even settings bracing piece between reel and the main part circle is connected, the axle center department of reel is equipped with reel commentaries on classics hole, the one end of connecting rod pass through the connecting piece with reel rotatable coupling, the other end of connecting rod with the upper end of support is connected.
As a preferred technical scheme, one end of the connecting piece is provided with a hinged joint, the other end of the connecting piece is provided with a rotating shaft, the diameter size of the outer surface of the rotating shaft is matched with the diameter size of the inner surface of the rotating hole of the shaft disc, one end of the connecting rod is hinged and connected with one end of the connecting piece through the hinged joint, and the rotating shaft at the other end of the connecting piece is inserted into the rotating hole of the shaft disc and is rotationally connected with the shaft disc.
The included angle between the shaft disc and the horizontal plane can be adjusted through the hinged connection mode, and then the included angle between the test tube sleeve and the horizontal plane is changed. So as to adjust the test tube arranged in the test tube sleeve to the angle convenient for inoculation.
As a preferred technical scheme, one end of the connecting rod is provided with a hinged joint, the hinged joint at one end of the connecting rod is connected with the hinged joint at one end of the connecting piece through a hinged bolt, and the hinged bolt is matched with a nut to fasten the hinged joint. The joint can be a straight joint, and the nut is a hand nut, such as a butterfly nut or a claw nut. When the angle adjusting device is used, the included angle between the shaft disc and the horizontal plane is adjusted, and then the nut is screwed to fix the included angle between the shaft disc and the horizontal plane.
As a preferred technical scheme, one end of the supporting rod is fixedly arranged on the outer side surface of the shaft disc, the other end of the supporting rod is fixedly arranged on the main body ring, and the central axes of the shaft disc and the main body ring are overlapped. Preferably, the shaft disc, the support rod and the main body ring are on the same plane, and a disc-shaped structure is formed by the shaft disc, the support rod and the main body ring; or the shaft disc, the support rod and the main body ring are not on the same plane, and a cone structure is formed by the shaft disc, the support rod and the main body ring.
As a preferred technical scheme, the upper end of the bracket is provided with a connecting clamp, and the other end of the connecting rod is fixed through the connecting clamp; further preferably, the connecting rod is vertically connected with the bracket.
As a preferred technical scheme, the support is designed into a telescopic support for adjusting the height of the inoculation frame.
The diameter of the main body ring can be adjusted according to needs, and the number of the test tube sleeves arranged on the main body ring can be adjusted according to the size of the main body ring; for example, the diameter of the main body ring is designed to be 90mm, and the material can be plastic or metal; the number of the test tube sleeves is designed to be 8, and the diameter of each test tube sleeve is 20-22 mm; but is not limited thereto.
The bracing piece play the effect of connecting main part circle and reel, the quantity of bracing piece can be adjusted according to actual conditions as long as can guarantee the connection stability of main part circle and reel can, the material is plastics or metal. For example, the number of the support rods is designed to be 4, but is not limited thereto.
The size and specification of the connecting rod can be adjusted according to actual conditions, and the connecting rod is made of plastic or metal. For example, the length of the connecting rod is 100mm, but is not limited thereto. The method for inoculating the edible fungi by adopting the device comprises the following steps
The method comprises the following steps: fixedly arranging an inoculation frame on the bracket;
step two: respectively placing test tubes to be inoculated and containing slant culture media into test tube sleeves, and keeping the slant upwards;
step three: adjusting the height of the inoculation rack from the alcohol lamp, and adjusting the angle between the test tube and the horizontal plane and the height from the test tube to the alcohol;
step four: the slant culture medium is sequentially inoculated by rotating the main body ring.
The invention has the beneficial effects that:
aiming at the technical problems of complex operation, time consumption and easy bacterial contamination in the microbial inoculation process in the prior art, the invention designs the simplified edible fungus inoculation device, which can achieve the effect of batch inoculation, effectively simplifies the inoculation operation, improves the inoculation efficiency, reduces the probability of bacterial contamination of a slant culture medium, has simple required equipment, simple operation and strong operability, and is suitable for wide popularization and application.
Drawings
FIG. 1 is a front view of an edible fungus inoculating device.
FIG. 2 is a side view of the edible fungus inoculating device.
Fig. 3 is a structural view of the connector.
Fig. 4 is a front view of the stand and base.
Wherein: 1-main part circle 2-test tube box 3-bracing piece 4-shaft dish 5-connecting rod 6-support 7-base 8-connecting piece 41-shaft dish commentaries on classics hole 61-connecting clamp 51, 81-articulated head 82-connecting axle 83-articulated bolt 84-nut.
Detailed Description
As shown in fig. 1-4, a simple edible fungus inoculation device comprises an inoculation rack, a base 7 and a support 6 fixedly arranged on the base, wherein the inoculation rack comprises a connecting rod 5, a supporting rod 3, a shaft disc 4, a main body ring 1 and a test tube sleeve 2 fixedly arranged on the main body ring, the shaft disc 4 and the main body ring 1 are uniformly arranged between the shaft disc 4 and the main body ring 1 through a plurality of supporting rods 3, a shaft disc rotating hole 41 is arranged at the axis of the shaft disc 4, one end of the connecting rod 5 is rotatably connected with the shaft disc 4 through a connecting piece 8, and the other end of the connecting rod 5 is connected with the upper end of the support 6. As a detailed technical scheme, one end of the connecting piece 8 is provided with a hinge 81, the other end of the connecting piece is provided with a rotating shaft 82, the diameter of the outer surface of the rotating shaft 82 is matched with the diameter of the inner surface of the shaft disc rotating hole 41, one end of the connecting rod 5 is hinged and connected with one end of the connecting piece 8 through a hinge 51, and the rotating shaft 82 at the other end of the connecting piece 8 is inserted into the shaft disc rotating hole 41 and is rotatably connected with the shaft disc 4. The included angle between the shaft disc 4 and the horizontal plane can be adjusted through the hinged connection mode, and then the included angle between the test tube sleeve 2 and the horizontal plane is changed. So as to adjust the test tube arranged in the test tube sleeve 2 to an angle convenient for inoculation. The joint 51 at one end of the connecting rod is connected with the joint 81 at one end of the connecting piece 8 through a hinge bolt 83, and the hinge bolt 83 is matched with a nut 84 to fasten the joints 81 and 51. The joints 81, 51 may be straight joints, and the nut 84 may be a hand nut, such as a wing nut or a claw nut. When the angle adjusting device is used, the included angle between the shaft disc 4 and the horizontal plane is adjusted, and then the nut 84 is screwed down to fix the included angle between the shaft disc 4 and the horizontal plane.
As a detailed scheme, one end of the support rod 3 is fixedly arranged on the outer side surface of the shaft disc 4, the other end of the support rod is fixedly arranged on the main body ring 1, and the central axes of the shaft disc 4 and the main body ring 1 are overlapped. The shaft disc 4, the support rod 3 and the main body ring 1 are not on the same plane, and a cone structure is formed by the shaft disc 4, the support rod 3 and the main body ring 1.
The upper end of the bracket 6 is provided with a connecting clamp 61, and the other end of the connecting rod 5 is fixed through the connecting clamp 61; the connecting rod 5 is vertically connected with the bracket 6. As a preferred technical scheme, the support 6 is designed into a telescopic support for adjusting the height of the inoculation frame.
The diameter of the main body ring 1 can be adjusted according to the requirement, and the number of the test tube sleeves 2 arranged on the main body ring 1 can be adjusted according to the size of the main body ring 1; for example, the diameter of the main body ring 1 is designed to be 90mm, and the material can be plastic or metal; the number of the test tube sleeves 2 is 8, and the diameter is 20-22 mm; but is not limited thereto.
The bracing piece 3 play the effect of connecting main part circle 1 and reel 4, the quantity of bracing piece 3 can adjust according to actual conditions as long as can guarantee main part circle 1 and reel 4's connection stability can, the material is plastics or metal. For example, the number of the support rods 3 is designed to be 4, but is not limited thereto.
The dimension of the connecting rod 5 can be adjusted according to actual conditions, and the material is plastic or metal. For example, the length of the connecting rod 5 is 100mm, but is not limited thereto.
The method for inoculating the edible fungi by adopting the device comprises the following steps
The method comprises the following steps: the inoculation rack is fixedly arranged on the bracket 6;
step two: respectively placing test tubes to be inoculated and containing slant culture media into the test tube sleeves 2, and keeping the slant upwards;
step three: adjusting the height of the inoculation rack from the alcohol lamp, and adjusting the angle between the test tube and the horizontal plane and the height of the test tube port of the test tube at the lowest part of the inoculation rack from the upper part of the flame of the alcohol lamp by 1-2 cm;
step four: the slant culture medium is sequentially inoculated by rotating the main body ring 1.
When the simplified edible fungus inoculation device is used, a test tube containing a slant culture medium is placed in the test tube sleeve 2, the horizontal height of an inoculation frame is adjusted by using the telescopic support 6, a slant culture base surface is enabled to face upwards by using the rotatable shaft disc 4, the test tube and the horizontal plane keep a proper angle, the distance between the lowest test tube opening of the inoculation frame and the upper part of an alcohol lamp flame is 1-2cm, the test tube is ensured to be in a sterile environment, after one test tube is inoculated, the position of the test tube is changed by using the rotatable shaft disc 4, and other slant culture mediums are sequentially inoculated. The device simplifies the inoculation operation, improves the inoculation efficiency and reduces the chance of the infection of the slant culture medium.
Claims (8)
1. The utility model provides a simple and easy domestic fungus inoculation device, its characterized in that, the device is in including inoculation frame, base and fixed setting support on the base, the inoculation frame include connecting rod, bracing piece, reel, main part circle and fixed setting test tube cover that the main part circled, reel and main part circle be in through many even settings bracing piece between reel and the main part circle is connected, the axle center department of reel is equipped with reel commentaries on classics hole, the one end of connecting rod pass through the connecting piece with reel rotatable coupling, the other end of connecting rod with the upper end of support is connected.
2. The device as claimed in claim 1, wherein one end of the connecting member is provided with a hinge joint, the other end of the connecting member is provided with a rotating shaft, the diameter of the outer surface of the rotating shaft is matched with the diameter of the inner surface of the rotating hole of the shaft disc, one end of the connecting rod is connected with one end of the connecting member in a hinged mode through the hinge joint, and the rotating shaft at the other end of the connecting member is inserted into the rotating hole of the shaft disc to be connected with the shaft disc in a rotating mode.
3. The device of claim 1, wherein one end of the support rod is fixedly arranged on the outer side surface of the shaft disc, the other end of the support rod is fixedly arranged on the main body ring, and the central axes of the shaft disc and the main body ring are coincided.
4. The device as claimed in claim 3, wherein the hub, the support rod and the body ring are in the same plane, and a disc-shaped structure is formed by the hub, the support rod and the body ring; or the shaft disc, the support rod and the main body ring are not on the same plane, and a cone structure is formed by the shaft disc, the support rod and the main body ring.
5. The device according to claim 1, wherein the joint at one end of the connecting rod is connected with the joint at one end of the connecting piece through a hinge bolt, and the hinge bolt is matched with a nut to fasten the joints.
6. The device as claimed in claim 1, wherein the bracket is provided at an upper end thereof with a coupling clip by which the other end of the connecting rod is fixed; the connecting rod is vertically connected with the bracket.
7. The apparatus of claim 1, wherein the stand is configured as a telescoping stand for adjusting the height of the inoculating rack.
8. A method of inoculating edible fungi using the apparatus of claim 1, wherein: comprises the following steps
The method comprises the following steps: fixedly arranging an inoculation frame on the bracket;
step two: respectively placing test tubes to be inoculated and containing slant culture media into test tube sleeves, and keeping the slant upwards;
step three: adjusting the height of the inoculation rack from the alcohol lamp, and adjusting the angle between the test tube and the horizontal plane and the height from the test tube to the alcohol;
step four: the slant culture medium is sequentially inoculated by rotating the main body ring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110535304.8A CN113207556A (en) | 2021-05-17 | 2021-05-17 | Simplified edible fungus inoculation device and inoculation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110535304.8A CN113207556A (en) | 2021-05-17 | 2021-05-17 | Simplified edible fungus inoculation device and inoculation method |
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| CN113207556A true CN113207556A (en) | 2021-08-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110535304.8A Pending CN113207556A (en) | 2021-05-17 | 2021-05-17 | Simplified edible fungus inoculation device and inoculation method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100777494B1 (en) * | 2006-06-09 | 2007-11-29 | 채정윤 | Support for a test tube |
| CN202893398U (en) * | 2012-11-28 | 2013-04-24 | 黑龙江八一农垦大学 | Rotatable test tube rack used for inclined surface bacteria inoculation |
| CN108686732A (en) * | 2018-07-27 | 2018-10-23 | 山东大学齐鲁医院 | A kind of rotatable test tube holder |
| CN217790703U (en) * | 2021-05-17 | 2022-11-15 | 南京农业大学 | Simplified edible mushroom inoculation device |
-
2021
- 2021-05-17 CN CN202110535304.8A patent/CN113207556A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100777494B1 (en) * | 2006-06-09 | 2007-11-29 | 채정윤 | Support for a test tube |
| CN202893398U (en) * | 2012-11-28 | 2013-04-24 | 黑龙江八一农垦大学 | Rotatable test tube rack used for inclined surface bacteria inoculation |
| CN108686732A (en) * | 2018-07-27 | 2018-10-23 | 山东大学齐鲁医院 | A kind of rotatable test tube holder |
| CN217790703U (en) * | 2021-05-17 | 2022-11-15 | 南京农业大学 | Simplified edible mushroom inoculation device |
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