CN113170787A - Inducer for codling moth and application thereof - Google Patents
Inducer for codling moth and application thereof Download PDFInfo
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- CN113170787A CN113170787A CN202110499847.9A CN202110499847A CN113170787A CN 113170787 A CN113170787 A CN 113170787A CN 202110499847 A CN202110499847 A CN 202110499847A CN 113170787 A CN113170787 A CN 113170787A
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- codling moth
- inducer
- codling
- octadiene
- dimethyl
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
Abstract
The invention discloses an inducer for codling moth, which comprises the components of 3, 7-dimethyl-1, 6-octadiene-3-ol (DMOD) and (E, E) -8, 10-dodecadiene-1-ol (CMP) in a weight ratio of 1:8-9: 1. The cydia pomonella inducer is safe and reliable, has the attraction effect on cydia pomonella male adults by the low-dose inducer, has the passivation effect on the central nervous system of cydia pomonella male adults by the high-dose inducer, and can reduce or even eliminate the sensation and behavior reaction, so that the female insect can not make various behavior reactions such as directional flight, wing flapping, copulation and the like.
Description
Technical Field
The invention relates to the technical field of insect central nervous system guidance, in particular to a central nervous system inducer of codling moth and application thereof.
Background
Cydia pomonella (L.) is a world-known invasive pest belonging to lepidoptera, tortricidae, and tortricidae, mainly eating fruits by larvae, and the seeds play a very important role in the development of the Cydia pomonella, so that the Cydia pomonella larvae have the characteristic of being lopsided to the seeds. The insect generates 1-3 generations in China in one year, and 45-54 days are needed for completing the 1 generation. The larvae in the 1 st generation have diapause, and the 1 st generation of the larvae is completed in 1 year. 2 generations can be completed in 1 year, and some can develop to the 3 rd generation. Generally 1 larva can harm 1-4 fruits, is a destructive pest of fruit trees such as apples, pears, Chinese pear, peaches, apricots and the like, and is also listed as an international quarantine object.
For a long time, the prevention and control means aiming at the codling moth mainly depends on the traditional chemical pesticide spraying for prevention and control, and the pheromone lures the core to lure for dynamic monitoring of the codling moth population.
Disclosure of Invention
The invention aims to provide an inducer for codling moth and application thereof, wherein the inducer with low dose can stimulate the central nervous system of male codling moth and attract male imagoes of the codling moth, and the inducer with high dose has a passivation effect on the central nervous system of the male imagoes of the codling moth, so that the sensory and behavioral reactions of the codling moth can be reduced or even disappear, and the female imagoes can not be found to make various behavioral reactions such as directional flight, winging, copulation and the like.
In order to achieve the purpose, the invention provides an inducer for codling moth, which comprises the components of 3, 7-dimethyl-1, 6-octadiene-3-ol and (E, E) -8, 10-dodecadiene-1-ol in a weight ratio of 1:8-9: 1.
The application of the induction agent for codling moth is characterized in that when the induction agent is prepared according to the weight ratio of the 3, 7-dimethyl-1, 6-octadiene-3-ol to the (E, E) -8, 10-dodecadiene-1-ol of 1:8-9:1, the induction agent has an induction or passivation effect on codling moth.
Preferably, the inducer prepared by 3, 7-dimethyl-1, 6-octadiene-3-ol and (E, E) -8, 10-dodecadiene-1-ol in high dose has passivation effect on the central nervous system of codling moth.
Preferably, when the weight ratio of the 3, 7-dimethyl-1, 6-octadiene-3-ol to the (E, E) -8, 10-dodecadiene-1-ol is 1:8-9:1, the central nervous system passivation effect on male moths of codling moths is more obvious than that of female moths.
Preferably, the inducer prepared by 3, 7-dimethyl-1, 6-octadiene-3-ol and (E, E) -8, 10-dodecadiene-1-ol in low dose has attraction effect on adult male codling moth.
Preferably, when the weight ratio of the 3, 7-dimethyl-1, 6-octadiene-3-ol to the (E, E) -8, 10-dodecadiene-1-ol is 1:8-9:1, the compound is prepared at a low dose, the compound has more obvious passivation effect on the central nervous system of male moths of codling moths than female moths.
Preferably, the minimum amount of the inducer which can inactivate the central nervous system of codling moth is 50 mg.
Preferably, the induction agent has a good effect of attracting male moths of codling moths when the induction agent is 0.1-1.1 mg.
A preparation method of an attractant core for attracting codling moth comprises using a cuff type composite rubber plug as a slow release carrier, injecting an attractant into the cuff of the rubber plug, sealing and standing for 30min at normal temperature until the rubber plug completely absorbs a mixed solution.
A method for preparing a central nervous system inducer of codling moth uses a PVC liquid filling tube, a PE adhesive tape, a timing spray device or a slow-release paste as a slow-release carrier, and the attractant is thermally sealed in the slow-release carrier.
Therefore, the induction agent for codling moth and the application thereof are safe and reliable, the induction agent with low dosage has an attraction effect on male adult codling moth, the induction agent with high dosage can passivate the central nervous system of the male adult codling moth, desensitize the male adult codling moth, and find the female adult codling moth which does not lay eggs and does not copulate, so that the effects of reducing the copulation and egg laying probability of the female and male adult codling moths in the field and reducing the population quantity of the codling moth are achieved.
The technical solution of the present invention is further described in detail by the following examples.
Detailed Description
The technical solution of the present invention is further illustrated by the following examples.
Example one
Attraction test for binding of different compounds to sex pheromones
A series of attractants are prepared by mixing different compounds with codling moth sex pheromone (E, E) -8, 10-dodecadien-1-ol (hereinafter referred to as CMP) and are matched with a trap for use, and a pure codling moth pheromone lure core is used as a control for testing.
The luring test is carried out in an apple planting base near Lanzhou city in Gansu province, the apple tree of the base is at the stage of the beginning of flowering, the luring test is judged according to the occurrence condition of the insect pest in the past, and the time period is usually the beginning period of the codling moth.
Dissolving 1mg of a contrast compound and 0.1mg of CMP in 0.1ml of n-hexane solvent, injecting the mixed solution into a cuff type composite rubber plug (hereinafter referred to as a rubber plug), sealing and standing until the mixed solution is completely absorbed, and forming the moth attractant core of the codling moth contrast attractant. The lure core is placed on a pest sticking plate at the bottom of the triangular trap and hung on the branches of the apple trees 1.5 meters away from the ground, 5 treatments are repeated every time, and the treatments are arranged at intervals of 3 rows. And counting the number of the codling moths trapped in the trap at an interval of 28 days after the trapping device is set, and then withdrawing the trapping device.
TABLE 1 Lasioderma pomonella comparative attractant Lasioderma pomonella trapping amount of different mixed components
Note: the same letters indicate that the difference is not significant.
The test results in Table 1 show that 1mg of 3, 7-dimethyl-1, 6-octadiene-3-ol (hereinafter referred to as "DMOD"), cis-folate and farnesene mixed with 0.1mg of CMP solution have stronger attraction effect on codling moth compared with the high-concentration and low-concentration pure codling moth sex pheromone attraction cores. The other contrast attractants also have some attracting effects on codling moth, but the effect is worse than that of a pure sex pheromone attracting core. The higher the sex pheromone concentration, the stronger the luring core has the luring ability to codling moth. However, the volatilization rates of both cis-folate acetate and farnesene are too fast to be suitable as long-acting attractant components. Thus, the superiority of DMOD and CMP compositions is known.
Example two
Low dose CMP and DMOD compounding ratio screening assay
Low-dose CMP and DMOD are mixed and dissolved in n-hexane solvent according to different proportions to prepare the trapping cores of the codling moth contrast attractant with different proportions, and the trapping capacity of the codling moth contrast attractant with the DMOD and the CMP mixed in different proportions to the codling moth is tested in the same field suspension mode as in the first embodiment.
Table 2 Dendrolimus punctatus trapping amount as comparative attractant for Dendrolimus punctatus (DMOD) and CMP mixed in different proportions
Note: the same letters indicate that the difference is not significant.
The test results in table 2 show that the combination of CMP and DMOD with a certain dosage is reduced, and the attraction capability to codling moth is equivalent to that of CMP used alone, but no attraction capability to codling moth is realized by using DMOD alone.
EXAMPLE III
Testing the influence of the combination of high-dose CMP and DMOD at different ratios on the ability of male codling moth to search for male and female codling moth
A PVC pipe is selected as a long-acting slow-release carrier (in the third and fourth effect tests, a well-known mature volatile mixture slow-release technology-a PVC liquid filling pipe is selected temporarily), soybean oil is used as a solvent, and a high-dose mixed solution (110mg/PVC pipe) of CMP and DMOD in different proportions is added into 0.2ml of soybean oil to be thermally sealed in the PVC pipe. The apple orchard is divided into 9 pieces according to the shape of a Chinese character jing, a triangular trap with 1.0mg of CMP rubber plug lure cores (as lure cores for monitoring) is placed in a field in the center, 2 PVC pipes are placed in each field in the 9 fields, a certain distance is kept between every two PVC pipes, and excessive approach is avoided. Each treatment was set to 5 replicates and the insect attraction in each monitoring trap was investigated after 28 days.
Table 3 monitoring of trap insect attraction in combination treatment of high dose CMP and DMOD at different ratios
Note: the same letters indicate that the difference is not significant.
The test results in table 3 show that the mixed solution of CMP and DMOD in different proportions with high dose has a passivation effect on the central nervous system of codling moth male adults, so that the mixed solution can not find out various behavioral reactions such as directional flight, winging and copulation and the like of female moths, the egg falling amount in the field is reduced, the population number is reduced, and the combined use of DMOD and CMP with lower dose can enable the passivation degree of the central nervous system of codling moth male adults to be almost equal to the degree which can be achieved by using high dose CMP alone, and the passivation effect on the central nervous system of codling moth is optimal when the weight ratio of CMP to DMOD is within the range of 1:8-9: 1.
Example four
Test experiment for central nervous system passivation effect of CMP and DMOD mixed solution with different weight ratios on male codling moth adults
A PVC tube is selected as a long-acting slow-release carrier, soybean oil is selected as a solvent, and 0.2ml of soybean oil is added into mixed liquid (50mg/PVC tube) of CMP and DMOD in different weight ratios and is heat-sealed in the PVC tube. An apple orchard is divided into 9 blocks according to the shape of a Chinese character jing, a triangular trap with 1.0mg of CMP rubber plug lure cores (as lure cores for monitoring) is placed in a field block at the center, 2 PVC pipes are placed in each field block in the 9 field blocks, a certain distance is kept between every two PVC pipes, and the PVC pipes are prevented from being very close to each other. Each treatment was set to 4 replicates and the insect attraction in each monitoring trap was investigated after 28 days.
Table 4 monitoring of insect attraction of traps in combined treatment of CMP and DMOD at different weight ratios
Note: the same letters indicate that the difference is not significant.
The test results in Table 4 show that the low-dose CMP solution also has a good passivation effect on the central nervous system of the male codling moth after a certain dose of DMOD is supplemented, but the pure DMOD solution has no any guiding effect on the behavioral response of the male codling moth adults.
EXAMPLE five
Testing the attraction effect by adopting different slow-release modes
In the foregoing experiments, a PVC pipe was used as the controlled-release device, but in addition to the PVC pipe, an effective controlled-release device such as a PE strip, a time spray device, a controlled-release ointment (adhesive, vegetable oil) and the like may be used. Through the experimental results, 110mg of the CMP and DMOD combined solution with the weight ratio of 4:1 is added into slow-release carriers such as a PVC pipe, a PE adhesive tape, a timing spraying device (when the device is used, the CMP and DMOD combined solution needs to be diluted and sprayed, and the slow-release carriers can be continuously sprayed for one month and slow-release paste on the premise that the spraying is not more than 48 times per day).
And selecting an apple orchard with consistent area, terrain, growth state and insect pest occurrence condition for each treatment, and repeating the treatments for 5 times. And setting a test area according to the test mode, and placing a PVC pipe, a PE rubber pipe and a timing spraying device. The slow release paste is uniformly coated at 18 points per block, each point is coated with 2ml, and the coating position is 2/3 distance from the ground of the tree body. The amount of insects in the traps was monitored and recorded at 7D, 14D, 21D and 28D after treatment, respectively, and the insects in the traps were emptied each time the investigation. The total number of 5 replicates are in the table below.
TABLE 5 attraction effect of attractants made of different slow-release carriers on fruit flies
| Time \ treatment | Blank space | PVC pipe | PE rubber pipe | Timing spraying device | Sustained-release ointment |
| 7D | 6 | 0 | 0 | 0 | 0 |
| 14D | 9 | 0 | 2 | 0 | 0 |
| 21D | 8 | 0 | 0 | 0 | 1 |
| 28D | 13 | 0 | 0 | 1 | 0 |
The test results in table 5 show that the PVC pipe, the PE adhesive tape, the timing spray device, the slow-release paste and the like can be used as a long-acting slow-release device of the codling moth inducer, and the lasting time can reach more than 29 days.
Therefore, the induction agent for codling moth is safe and reliable, has an attraction effect on the codling moth male imagoes at a low dose, has a passivation effect on the central nervous system of the codling moth male imagoes at a high dose, and can prevent the codling moth from finding female imagoes to copulate and lay eggs.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.
Claims (9)
1. An inducer for codling moth, which is characterized in that: the inducer comprises 3, 7-dimethyl-1, 6-octadiene-3-alcohol and (E, E) -8, 10-dodecadiene-1-alcohol in a weight ratio of 1:8-9: 1.
2. The application of the codling moth inducer is characterized in that: the inducer has the effect of attracting or inactivating codling moth when being prepared according to the weight ratio of 3, 7-dimethyl-1, 6-octadiene-3-ol to (E, E) -8, 10-dodecadiene-1-ol in claim 1 of 1:8-9: 1.
3. The use of the induction agent for codling moth according to claim 2, wherein: the inducer prepared from 3, 7-dimethyl-1, 6-octadiene-3-ol and (E, E) -8, 10-dodecadiene-1-ol in high dose has passivation effect on the central nervous system of codling moth.
4. The use of the induction agent for codling moth according to claim 2, wherein: when the weight ratio of the 3, 7-dimethyl-1, 6-octadiene-3-ol to the (E, E) -8, 10-dodecadiene-1-ol is 1:8-9:1, the compound is prepared at a high dose, the compound has more obvious passivation effect on the central nervous system of male codling moth than female moths.
5. The use of the induction agent for codling moth according to claim 2, wherein: the inducer prepared from 3, 7-dimethyl-1, 6-octadiene-3-ol and (E, E) -8, 10-dodecadiene-1-ol in low dose has an attraction effect on codling moth males.
6. The use of the induction agent for codling moth according to claim 2, wherein: when the weight ratio of the 3, 7-dimethyl-1, 6-octadiene-3-ol to the (E, E) -8, 10-dodecadiene-1-ol is 1:8-9:1, the attraction effect on male moths of codling moths is more obvious than that of female moths.
7. The use of the induction agent for codling moth according to claim 2, wherein: the minimum usage amount of the inducer which has the passivation effect on the central nervous system of codling moth is 50 mg.
8. The use of the induction agent for codling moth according to claim 2, wherein: the inducing agent has good effect of inducing male moth of codling moth when the amount of the inducing agent is 0.1-1.1 mg.
9. A method for preparing a central nervous system inducer of codling moth according to claim 1, characterized in that: a PVC liquid filling pipe, a PE adhesive tape, a timing spraying device or a slow-release paste is used as a long-acting slow-release device.
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| CN202110499847.9A CN113170787A (en) | 2021-05-08 | 2021-05-08 | Inducer for codling moth and application thereof |
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| CN202110499847.9A CN113170787A (en) | 2021-05-08 | 2021-05-08 | Inducer for codling moth and application thereof |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101072501A (en) * | 2004-10-13 | 2007-11-14 | Efal化学工业有限公司 | Agents for control of codling moth in fruit orchards |
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- 2021-05-08 CN CN202110499847.9A patent/CN113170787A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101072501A (en) * | 2004-10-13 | 2007-11-14 | Efal化学工业有限公司 | Agents for control of codling moth in fruit orchards |
Non-Patent Citations (4)
| Title |
|---|
| D´ENES SCHMERA ET AL: ""Plant volatile compounds shorten reaction time and enhance attraction of the codling moth (Cydia pomonella) to codlemone"", 《PEST MANAG SCI》 * |
| ZHIHUA YANG ET AL: ""HOST PLANT VOLATILES SYNERGIZE RESPONSE TO SEX", 《JOURNAL OF CHEMICAL ECOLOGY》 * |
| 姚永生等: "《苹果蠹蛾和梨小食心虫信息素及其应用》", 30 October 2018, 中国农业大学出版社 * |
| 翟小伟等: "苹果蠹蛾性信息素的研究和应用进展", 《昆虫学报》 * |
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