CN113154127A - Liquid storage and controlled release assembly and microfluidic detection chip - Google Patents
Liquid storage and controlled release assembly and microfluidic detection chip Download PDFInfo
- Publication number
- CN113154127A CN113154127A CN202110428959.5A CN202110428959A CN113154127A CN 113154127 A CN113154127 A CN 113154127A CN 202110428959 A CN202110428959 A CN 202110428959A CN 113154127 A CN113154127 A CN 113154127A
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- China
- Prior art keywords
- metal ring
- liquid storage
- controlled release
- release assembly
- storage device
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- 239000007788 liquid Substances 0.000 title claims abstract description 97
- 238000003860 storage Methods 0.000 title claims abstract description 81
- 238000013270 controlled release Methods 0.000 title claims abstract description 46
- 238000001514 detection method Methods 0.000 title claims abstract description 20
- 239000002184 metal Substances 0.000 claims abstract description 163
- 229910052751 metal Inorganic materials 0.000 claims abstract description 163
- 239000012188 paraffin wax Substances 0.000 claims abstract description 66
- 239000000758 substrate Substances 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 4
- 229920002457 flexible plastic Polymers 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 6
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 15
- 238000010438 heat treatment Methods 0.000 description 30
- 238000002844 melting Methods 0.000 description 5
- 230000008018 melting Effects 0.000 description 5
- 238000009434 installation Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000003466 welding Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical group [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000007731 hot pressing Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
Images
Classifications
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
- F16K99/0003—Constructional types of microvalves; Details of the cutting-off member
- F16K99/003—Valves for single use only
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
- F16K99/0003—Constructional types of microvalves; Details of the cutting-off member
- F16K99/0032—Constructional types of microvalves; Details of the cutting-off member using phase transition or influencing viscosity
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
- F16K99/0034—Operating means specially adapted for microvalves
- F16K99/0042—Electric operating means therefor
- F16K99/0044—Electric operating means therefor using thermo-electric means
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
- F16K99/0034—Operating means specially adapted for microvalves
- F16K99/0063—Operating means specially adapted for microvalves using centrifugal forces
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K2099/0082—Microvalves adapted for a particular use
- F16K2099/0084—Chemistry or biology, e.g. "lab-on-a-chip" technology
Abstract
The invention provides a liquid reagent storage and controlled release device, which is used for realizing timed and controllable release after liquid storage, has no liquid residue and reduces the influence of a residual reagent on the accuracy of subsequent detection. In order to achieve the purpose, the invention discloses a liquid storage and controlled release assembly which comprises a liquid storage device and a metal ring paraffin valve, wherein the metal ring paraffin valve consists of a metal ring and paraffin filled in the metal ring, an opening of the liquid storage device is matched with the outer diameter of the metal ring, and the metal ring paraffin valve blocks the opening of the liquid storage device. The liquid storage device is of a bottle-shaped structure, and the metal ring is of a cylindrical hollow annular structure.
Description
Technical Field
The invention relates to the field of microfluidic detection chips, in particular to a liquid storage and controlled release device in a microfluidic detection chip and a related microfluidic detection chip.
Background
With the advancement of medical technology, the demand for medical equipment is becoming more and more diversified. Under the condition, medical equipment based on the microfluidic technology is continuously developed and put into use, wherein the point-of-care diagnostic equipment (POCT) is particularly prominent. Centrifugal force microfluidics plays an important role in POCT, and its main advantages are the modular arrangement of the system and the disposable, easily replaceable microfluidic detection chip, as well as many existing cell operations, which make liquid handling very accurate. The reaction flow of the sample is implemented in the chip, which makes the pre-storage of the reagent a key factor for the use of the chip.
At present, liquid storage reagents in centrifugal force microfluidic detection chips generally break through various valves, such as capillary valves and siphon valves, by means of different rotating speeds. However, this method is unstable and cannot release a plurality of agents separately at different times. These disadvantages make the chip unable to integrate more reaction flows, and simplify the sample processing. This is not conducive to the development of the products of the microfluidic chip technology toward miniaturization, function integration and easy operation. And the liquid reagent storage can not ensure the liquid to be completely released, and liquid residue exists. Under the driving of centrifugal force, a plurality of reagents in the microfluidic detection chip are released, and residual reagents have outflow risks in subsequent centrifugation, so that the accuracy of subsequent detection is influenced. Therefore, achieving timed and stable release of liquid after storage is a technical problem which needs to be solved urgently in the field.
Disclosure of Invention
The invention aims to provide a liquid reagent storage and controlled release device, which is used for realizing timed and controllable release after liquid storage, has no liquid residue and reduces the influence of the residual reagent on the accuracy of subsequent detection. In order to achieve the purpose, the invention provides a liquid storage and controlled release assembly which comprises a liquid storage device and a metal ring paraffin valve, wherein the metal ring paraffin valve consists of a metal ring and paraffin filled in the metal ring, an opening of the liquid storage device is matched with the outer diameter of the metal ring, and the metal ring paraffin valve blocks the opening of the liquid storage device. The liquid storage device is of a bottle-shaped structure, and the metal ring is of a cylindrical hollow annular structure.
Furthermore, the metal ring is set to be a cylindrical or conical hollow annular structure, and the thinner end of the conical metal ring faces the inner cavity of the liquid storage device. When the metal ring is in a conical hollow annular structure, the paraffin valve is easier to open under the centrifugal force after heating, but is easier to be accidentally broken through than the paraffin valve with a cylindrical metal structure during transportation, and can be selected according to specific requirements. The liquid storage device is made of rubber or flexible plastic materials, and can also be in a bottle shape made of rigid materials.
The liquid storage and controlled release assembly is applied to a centrifugal force-based microfluidic detection chip, the centrifugal force microfluidic detection chip comprises a substrate and a liquid storage and controlled release assembly arranged in the substrate, a rotating shaft connecting hole is formed in the center of the substrate, a liquid flow pipeline is arranged in the substrate, a liquid storage and controlled release assembly mounting cavity is further arranged in the substrate, the liquid storage and controlled release assembly mounting cavity is communicated with a downstream liquid flow pipeline, the downstream liquid flow pipeline is opened at the radial far end of the liquid storage and controlled release assembly mounting cavity, the liquid storage and controlled release assembly is placed in the liquid storage and controlled release assembly mounting cavity, and the opening of the liquid storage and controlled release assembly extends into the downstream liquid flow pipeline opening.
Furthermore, a heat-conducting metal block is arranged on one side or two sides of a metal ring in the metal ring paraffin valve, which is close to the substrate, one side of the heat-conducting metal block, which is close to the metal ring, is embedded with the metal ring, and the opposite side is a plane, which is beneficial to heat conduction. The metal ring and the heat-conducting metal block in the metal ring paraffin valve can also be arranged into an integral structure. And a heat-conducting metal sheet can be arranged on the substrate at a position corresponding to the heat-conducting metal block.
The liquid is stored in a liquid storage device.
Drawings
FIG. 1 is a side view of a metal ring paraffin valve
FIG. 2 is a top view of a metal ring paraffin valve
FIG. 3 is a top view of the assembled metal ring paraffin valve
FIG. 4 is a side view of the assembled metal ring paraffin valve
FIG. 5 is a side view of a metal ring paraffin valve with a metal ring and a heat conducting metal block integrated
FIG. 6 is a top view of a metal ring paraffin valve with a metal ring and a heat conducting metal block integrated
FIG. 7 is a front view of the assembled metal ring paraffin valve with the metal ring and the heat conducting metal block as an integral structure
FIG. 8 is a side view of the metal ring paraffin valve assembled with the metal ring and the heat conductive metal block as an integral structure
FIG. 9 is a top view of the metal ring paraffin valve assembled with the metal ring and the heat conductive metal block as an integral structure
FIG. 10 is a sectional view showing the state where the liquid storage and controlled release assembly is mounted in the "liquid storage and controlled release assembly mounting chamber
FIG. 11 is a plan view showing the state where the liquid storage and controlled release assembly is installed in the "liquid storage and controlled release assembly installation chamber
FIG. 12 is a sectional view showing the state where the liquid storage and controlled release member is mounted in the "liquid storage and controlled release member mounting chamber" (the metal ring and the heat conductive metal block are not provided as an integral structure, and the circuit board is provided thereunder)
FIG. 13 is a plan view showing the state where the liquid storage and controlled release member is mounted in the "liquid storage and controlled release member mounting chamber" (the metal ring and the heat conductive metal block are not provided as an integral structure, and the circuit board is provided thereunder)
FIG. 14 is a sectional view of the integrally packaged metal ring paraffin valve (the metal ring and the heat-conducting metal block are integrated)
FIG. 15 is a top view of the integrally packaged metal ring paraffin valve (with the metal ring and the heat-conducting metal block configured as an integral structure)
FIG. 16 shows the opening of a metal ring paraffin valve by heating and centrifugal force (the metal ring and the heat conductive metal block are integrated)
FIG. 17 shows the liquid in the liquid storage device breaking through the metal ring paraffin valve and entering the downstream liquid flow pipe (the metal ring and the heat conducting metal block are integrated) after the metal ring paraffin valve is opened by heating and centrifugal force
In the figure: 100 is a substrate, 200 is a metal ring paraffin valve, 201 is a heat conducting metal block, 210 is solid paraffin, 300 is a liquid storage device, 400 is a heating device, 401 is a heating electronic device, 402 is a heating resistor, 500 is a circuit board, 600 is a heat conducting metal sheet, 700 is a reagent, 800 is a liquid storage and controlled release component mounting cavity, and 810 is a liquid flow pipeline.
Detailed Description
The core of the invention is to provide a liquid storage controlled release device and a biological detection chip to realize liquid storage, timed and stable release, eliminate unstable release completely depending on centrifugal force and integrate more reaction processes.
In order that those skilled in the art will better understand the technical solution of the present invention, the present invention will be further described in detail with reference to the accompanying drawings.
Fig. 1 is a side view of a metal ring paraffin valve, and fig. 2 is a top view of the metal ring paraffin valve. The metal ring is in a cylindrical or conical hollow annular structure, and when the metal ring is in the conical hollow annular structure, the thinner end of the conical metal ring faces the inner cavity of the liquid storage device.
Fig. 3 is a plan view of the metal ring paraffin valve after assembly, and fig. 4 is a side view of the metal ring paraffin valve after assembly. The opening of the liquid storage device is matched with the outer diameter of the metal ring, the metal ring is partially inserted into the position about 1/2 of the opening of the bottle-shaped structure, and the metal ring paraffin valve blocks the opening of the liquid storage device.
Fig. 5 is a side view of a metal ring paraffin valve when the metal ring and the heat-conducting metal block are integrated, and fig. 6 is a top view of the metal ring paraffin valve when the metal ring and the heat-conducting metal block are integrated, wherein the metal ring has a cylindrical or conical hollow annular structure, and when the metal ring has a conical hollow annular structure, a thinner end of the conical metal ring faces an inner cavity of the liquid storage device. The middle upper part of the outer wall is provided with a heat-conducting metal block, the shape of the heat-conducting metal block is cuboid or cube convex, and the material of the heat-conducting metal block is consistent with that of the metal ring. The length of the protrusion is consistent with the length of the concave part of the substrate.
Fig. 7 is a front view of the assembled metal ring paraffin valve when the metal ring and the heat-conducting metal block are integrated, fig. 8 is a side view of the assembled metal ring paraffin valve when the metal ring and the heat-conducting metal block are integrated, fig. 9 is a top view of the assembled metal ring paraffin valve when the metal ring and the heat-conducting metal block are integrated, the opening of the liquid storage device is adapted to the outer diameter of the metal ring, and the length of the opening of the metal ring does not exceed the length of the bottle-shaped structure opening when the metal ring is inserted into the liquid storage device. The metal ring paraffin valve blocks the opening of the liquid storage device.
Example 1
Fig. 10 is a sectional view showing the liquid storage and controlled release assembly installed in the "liquid storage and controlled release assembly installation chamber" when the metal ring of the metal ring paraffin valve is not integrated with the heat conductive metal block. The substrate structure below the metal ring paraffin valve is a porous structure, and a heating device is connected in the structure and used for heating and melting paraffin. The bottle-shaped structure is packaged with a reagent in advance, and the middle of the metal ring is provided with sealing paraffin. A plane heating device is arranged below the metal ring. The metal ring is closely connected with the opening of the substrate liquid flow pipeline, after the heating device heats, the solid paraffin melts, the reagent is separated from the bottle-shaped structure through centrifugal force and enters the liquid flow pipeline through the metal ring paraffin valve.
Fig. 11 is a plan view of the liquid storage and controlled release module installed in a "liquid storage and controlled release module installation cavity" having a size equal to or larger than that of the bottle-shaped structure and a liquid flow pipe opening equal to or slightly larger than that of the metal ring paraffin valve. The liquid storage and controlled release component mounting cavity is positioned at the radial near end of the centrifugal force microfluidic detection chip, and the reagent flows to various other radial far end cavities through centrifugal force.
Example 2
Fig. 12 is a sectional view of the liquid storage and controlled release assembly installed in the "liquid storage and controlled release assembly installation cavity" (the metal ring and the heat conductive metal block are not integrated, and the circuit board is installed below the metal ring and the heat conductive metal block), at this time, the metal ring and the heat conductive metal block in the metal ring paraffin valve are not integrated, the substrate structure below the metal ring paraffin valve is a hole-shaped structure, and a heating device is connected to the structure for heating and melting paraffin. The heating device is connected with the circuit board below and is controlled to be started through the circuit system. The passive plate is essentially a circuit board to which heating means are soldered at a hole-like or hole-like structure below the respective substrate liquid storage and controlled release assembly mounting chamber by appropriate wiring.
Example 3
Fig. 14 is a cross-sectional view of the integrally packaged metal ring paraffin valve (the metal ring and the heat-conducting metal block are integrated), in which case the metal ring and the heat-conducting metal block in the metal ring paraffin valve are integrated. At the moment, the heat-conducting convex part of the metal ring can be clamped at the lower structure to play roles in positioning and fixing. And the raised portion of the metal ring is closely attached to the underlying structure. The structure of the substrate below the metal ring paraffin valve is a hole-shaped or hole-shaped structure, and a heating device is connected in the structure and used for heating and melting paraffin. The heating device is connected with the lower driven plate. The heating device is arranged as a heating resistor, and a heat-conducting metal sheet is arranged above the heating resistor for further improving the heating effect. The heat-conducting metal sheet and the heating resistor are glued together through the heat-conducting glue, and the heating resistor and the circuit board are connected together through welding. The heating resistor, the heat conducting metal sheet and the circuit board are packaged together into a whole to form a driven plate. The passive plate and the centrifugal force micro-fluidic detection chip are connected through ultrasonic welding, hot pressing or gluing and are packaged into a whole together.
Example 4
Fig. 16 shows the metal ring paraffin valve opened by heating and centrifugal force (the metal ring and the heat conductive metal block are integrated), and the metal ring and the heat conductive metal block are integrated in the metal ring paraffin valve. When the reagent needs to be released, the passive plate inputs current, and the heating resistor starts to work. The heat generated by the heating resistor is transmitted to the metal ring paraffin valve through the heat conducting metal sheet, the solid paraffin is heated and melted, and the liquid breaks through the valve due to the centrifugal force and enters the liquid flow pipeline.
Example 5
The metal ring paraffin valve uses fully refined low-melting-point solid paraffin, and the melting point is 48 ℃. The resistance working current is 250mA, the heating time is 30s, the heating is carried out to 51.2 ℃, and the heating speed is about 1.7 ℃/s. The heat conducting metal sheet is a copper sheet with the diameter of 6mm, and the heat conductivity coefficient is 381W/m.K. The paraffin wax starts to melt after being heated, and the time for complete melting is about 5 s. The motor rotates at 500rpm for 5s, so that the reagent is completely separated from the bottle-shaped structure into the liquid flow pipeline and flows into the next chamber. See fig. 17.
Claims (10)
1. The liquid storage and controlled release assembly is characterized by comprising a liquid storage device and a metal ring paraffin valve, wherein the liquid storage device is provided with an opening, the metal ring paraffin valve consists of a metal ring and paraffin filled in the metal ring, the opening of the liquid storage device is matched with the outer diameter of the metal ring, and the metal ring paraffin valve blocks the opening of the liquid storage device.
2. The fluid storage and controlled release assembly of claim 1, wherein the fluid storage device is a bottle-like structure.
3. The liquid storage and controlled release assembly of claim 1, wherein the metal ring is a cylindrical, or conical hollow ring structure.
4. The fluid storage and controlled release assembly of claim 1, wherein the metal ring is a conical hollow ring structure, the thinner end of the conical metal ring facing the interior cavity of the fluid storage device.
5. The fluid storage and controlled release assembly of claim 1, wherein the fluid storage device is made of rubber or flexible plastic.
6. The liquid storage and controlled release assembly of claim 1, wherein the liquid storage device is in the form of a bottle made of a rigid material.
7. A centrifugal force based microfluidic detection chip, comprising a substrate and the liquid storage and controlled release assembly according to any one of claims 1 to 6 disposed in the substrate, wherein the substrate is centrally provided with a rotating shaft connecting hole, the substrate is provided with a liquid flow pipeline, the substrate is further provided with a liquid storage and controlled release assembly mounting cavity, the liquid storage and controlled release assembly mounting cavity is communicated with a downstream liquid flow pipeline, the downstream liquid flow pipeline is opened at a radial far end of the liquid storage and controlled release assembly mounting cavity, the liquid storage and controlled release assembly according to any one of claims 1 to 6 is disposed in the liquid storage and controlled release assembly mounting cavity, and an opening of the liquid storage and controlled release assembly extends into an opening of the downstream liquid flow pipeline.
8. The centrifugal force-based microfluidic detection chip according to claim 7, wherein a heat conducting metal block is arranged on one side or both sides of the metal ring in the metal ring paraffin valve, the side of the heat conducting metal block, which is close to the metal ring, is embedded with the metal ring, and the opposite side is a plane.
9. The centrifugal force-based microfluidic detection chip according to claim 7, wherein the metal ring in the metal ring paraffin valve and the heat conductive metal block are provided as an integral structure.
10. The centrifugal force-based microfluidic detection chip according to any one of claims 8 or 9, wherein a heat conductive metal sheet is disposed on the substrate at a position corresponding to the heat conductive metal block.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110428959.5A CN113154127A (en) | 2021-04-21 | 2021-04-21 | Liquid storage and controlled release assembly and microfluidic detection chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110428959.5A CN113154127A (en) | 2021-04-21 | 2021-04-21 | Liquid storage and controlled release assembly and microfluidic detection chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113154127A true CN113154127A (en) | 2021-07-23 |
Family
ID=76867923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110428959.5A Pending CN113154127A (en) | 2021-04-21 | 2021-04-21 | Liquid storage and controlled release assembly and microfluidic detection chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113154127A (en) |
-
2021
- 2021-04-21 CN CN202110428959.5A patent/CN113154127A/en active Pending
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