CN113578405A

CN113578405A - Micro-fluidic chip

Info

Publication number: CN113578405A
Application number: CN202110995895.7A
Authority: CN
Inventors: 魏道舜; 程晓宇; 刘笔锋; 钱纯亘; 肖育劲; 陈鹏
Original assignee: Shenzhen Yhlo Biotech Co Ltd
Current assignee: Shenzhen Yhlo Biotech Co Ltd
Priority date: 2021-08-27
Filing date: 2021-08-27
Publication date: 2021-11-02
Also published as: WO2023025274A1

Abstract

The invention relates to a micro-fluidic chip. The microfluidic chip comprises a chip main body and a liquid bag storage part, wherein a liquid inlet is formed in the chip main body, the liquid bag storage part is positioned on the chip main body, a liquid bag cavity for placing a liquid bag is formed in the liquid bag storage part, the liquid bag cavity is provided with a liquid outlet, the liquid outlet is communicated with the liquid inlet, and a spine part is arranged in the liquid bag cavity; when the liquid sac is placed in the liquid sac cavity, a gap is reserved between the liquid sac and the spine piece; when the extrusion force applied to the liquid sac exceeds a preset value, the sharp-pricked piece can prick the liquid sac. The micro-fluidic chip can reduce the rejection rate of the chip and improve the test repeatability and stability of the chip.

Description

Micro-fluidic chip

Technical Field

The invention relates to the technical field of microfluidics, in particular to a microfluidic chip.

Background

POCT, point-of-care testing, is a test method that is performed at the sampling site and uses a portable analyzer and a kit to quickly obtain a test result. POCT is analyzed immediately on the sampling site, complex processing procedures of samples in laboratory inspection are omitted, and the POCT has the advantages of rapid result output and simplicity in use.

At present, POCT products mainly comprise a microfluidic chip and a reagent applied to the microfluidic chip for detection. The reagent storage mode of POCT products is divided into chip internal storage and chip external storage. The internal storage of the chip refers to the step of reagent filling introduced in the chip processing and assembling process, and the whole chip packaging is completed after the liquid reagent is directly injected into the storage cavity or the storage groove in the chip. When the chip is internally stored, the reagent is directly arranged in the chip without additionally and independently storing the reagent in a packaging mode, but the blocking or valve control is needed between the chip storage groove and the fluid channel in the chip, otherwise, the reagent flows into the channel in advance in the storage and transportation processes and is scrapped; moreover, reagent packaging and chip processing are combined together, so that the processing difficulty and the process control difficulty are increased, the process flow is long and complex, for example, the design complexity of the chip is increased by setting a valve control or a blocking, the introduction problem of the reagent in the testing process is ensured while the stability of the reagent storage and transportation process is considered, and the control complexity of the testing system is increased; in addition, generally, when a chip is used for internal storage, the types of stored reagents are limited, and the built-in of a multi-component reagent cannot be compatible.

The chip external storage means that the chip assembly and the reagent filling and packaging processes are independent, and after the liquid reagent is sealed in the liquid bag, the liquid bag is attached to the corresponding position of the chip and then assembled for use. When the chip is stored outside, the steps of reagent filling and packaging are separated from the steps of chip processing and assembling, the process control is more reliable, the storage of various reagents can be easily realized, and the flexibility and the degree of freedom of combination with the chip are high. However, the liquid bag containing the liquid reagent is attached to the outside of the chip, and is easily damaged by extrusion and discarded during transportation and storage, and has poor test repeatability and stability during use.

Disclosure of Invention

Therefore, a micro-fluidic chip capable of reducing the rejection rate and improving the test repeatability and stability is needed.

A microfluidic chip, comprising:

the chip comprises a chip main body, wherein a liquid inlet is formed in the chip main body; and

the liquid bag storage part is positioned on the chip main body, a liquid bag cavity for placing the liquid bag is formed in the liquid bag storage part, the liquid bag cavity is provided with a liquid outlet, the liquid outlet is communicated with the liquid inlet, and a spine part is arranged in the liquid bag cavity; when the liquid sac is placed in the liquid sac cavity, a space is reserved between the liquid sac and the spine piece; when the extrusion force applied to the liquid sac exceeds a preset value, the sharp-pointed piece can puncture the liquid sac.

According to the micro-fluidic chip, the liquid bag is spaced from the sharp piercing piece when the liquid bag is placed in the liquid bag cavity, so that the liquid bag is not pierced when being subjected to extrusion force (such as the extrusion force in the transportation and storage processes) not exceeding a preset value, and the liquid bag can be pierced when being subjected to the extrusion force exceeding the preset value in use, and therefore the rejection rate of the micro-fluidic chip is reduced. And the arrangement of the sharp thorn piece enables the flow velocity and the flow resistance of the reagent flowing out of the liquid bag to be basically the same, thereby improving the detection repeatability and the stability of the microfluidic chip.

In one embodiment, the spine piece is provided with a drainage groove which is communicated with the liquid outlet.

In one embodiment, the spike is tapered or needle-shaped.

In one embodiment, the microfluidic chip comprises a protection member, the protection member is connected to the chip main body and covers the liquid sac storage member, and the protection member is provided with a liquid sac avoiding hole corresponding to the liquid sac cavity.

In one embodiment, the microfluidic chip further comprises an indicator, the indicator is located between the sac storage member and the protection member, and the material of the indicator is a material which is easily deformed under stress; the protective piece is a transparent protective piece, and/or the material of the liquid sac storage piece and the material of the chip main body are both transparent materials.

In one embodiment, the material of the indicator is a plastic film, an aluminum foil, a tin foil or paper.

In one embodiment, the microfluidic chip further comprises a first fixing member for fixedly connecting the liquid bag storage member and the chip main body.

In one embodiment, the microfluidic chip further comprises a second fixing member for fixedly connecting the protection member and the sac storage member.

In one embodiment, the microfluidic chip further includes a sealing member located between the sac storage member and the chip body, the sealing member is used for sealing a gap between the sac storage member and the chip body, the sealing member has a liquid channel, and the liquid outlet is communicated with the liquid inlet through the liquid channel.

In one embodiment, in the microfluidic chip, the bottom of the liquid bag cavity is funnel-shaped, the spike member is located on the bottom, and the liquid outlet is located at the bottom closest to the chip body.

Drawings

FIG. 1 is a perspective view of a microfluidic chip according to an embodiment;

FIG. 2 is an exploded view of the microfluidic chip shown in FIG. 1;

fig. 3 is a perspective view of a fluid bladder storage member of the microfluidic chip shown in fig. 1;

FIG. 4 is a cross-sectional view of the microfluidic chip shown in FIG. 1;

FIG. 5 is an enlarged view of portion B of the fluid bladder storage element shown in FIG. 4;

FIG. 6 is an enlarged view of portion A of the fluid bladder storage member shown in FIG. 3;

fig. 7 is an exploded view of the microfluidic chip shown in fig. 1 before assembly.

Reference numerals:

10. a microfluidic chip; 110. a chip body; 120. a liquid bladder storage member; 111. a liquid inlet; 121. a liquid sac cavity; 122. a liquid outlet; 123. a spike member; 124. a drainage groove; 125. a seal member; 126. a liquid channel; 130. a liquid sac; 140. a protective member; 141. avoiding holes; 150. an indicator; 160. a first fixing member; 170. a second fixing member; 180. positioning holes; 161. a first fixed column; 171. and a second fixing column.

Detailed Description

The present invention will now be described more fully hereinafter for purposes of facilitating an understanding thereof, and may be embodied in many different forms and are not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may be present. When the terms "vertical," "horizontal," "left," "right," "upper," "lower," "inner," "outer," "bottom," and the like are used to indicate an orientation or positional relationship, it is for convenience of description only based on the orientation or positional relationship shown in the drawings, and it is not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

Referring to fig. 1 and 2, an embodiment of the present application provides a microfluidic chip 10, where the microfluidic chip 10 includes a chip main body 110 and a liquid bag storage part 120, a liquid inlet 111 is disposed on the chip main body 110, the liquid bag storage part 120 is located on the chip main body 110, a liquid bag cavity 121 for placing a liquid bag 130 is disposed on the liquid bag storage part 120, the liquid bag cavity 121 has a liquid outlet 122, the liquid outlet 122 is communicated with the liquid inlet 111, and a spike 123 is disposed in the liquid bag cavity 121; when the sac 130 is placed in the sac cavity 121, the sac 130 is spaced from the spike 123; when the pressing force applied to the sac 130 exceeds a predetermined value, the spike 123 can pierce the sac 130. By the spacing between the liquid bag 130 and the piercing member 123 when the liquid bag 130 is placed in the liquid bag cavity 121, the liquid bag 130 is not pierced by the piercing member 123 when the pressing force (e.g., the pressing force applied during transportation and storage) applied thereto does not exceed a preset value, and is pierced by the piercing member 123 when the pressing force exceeding the preset value is applied thereto during use, thereby reducing the rejection rate of the microfluidic chip 10. In addition, the rupture of the liquid bag in the traditional microfluidic chip is that the liquid bag is directly extruded to break the aluminum foil on one side close to the liquid outlet, and the liquid flows out. Therefore, the present application, through the arrangement of the spike 123, after the fluid bags 130 are subjected to the same amount of pressing force (or different pressing forces which are greater than the limit value for puncturing the fluid bags 130, because the pressing force does not change the size of the openings of the fluid bags 130 after exceeding the limit value, and thus the flow rate and the flow resistance are not affected), the sizes of the openings of the fluid bags 130 are substantially the same, so that the flow rate and the flow resistance of the reagent flowing out of each fluid bag 130 are substantially the same, and the detection repeatability and stability of the above-mentioned microfluidic chip 10 are improved.

Specifically, the preset value of the pressing force applied to the fluid bladder 130 when the spike 123 punctures the fluid bladder 130 is related to the material of the fluid bladder 130, the separation distance between the fluid bladder 130 and the spike 123 when the fluid bladder 130 is placed in the fluid bladder cavity 121, and the pressing force applied to the fluid bladder 130 during transportation and storage. The specific preset value can be set according to the material of the sac 130, the distance between the sac 130 and the spike 123 when the sac 130 is placed in the sac cavity 121, and the pressing force applied to the sac 130 during transportation and storage. It will be appreciated that the preset value of the pressing force is designed based on the force actually received by sac 130 in the approaching direction from sac 130 to piercing member 123. For example, in the illustrated embodiment, the preset value of the squeezing force is designed based on the amount of vertical downward force that the sac 130 is able to withstand. For example, if the sac 130 is subjected to a downward force, the downward force in the vertical direction is only a component of the downward force, and the sac 130 is not punctured as long as the magnitude of the component does not exceed a predetermined value.

In some embodiments, the material of the side of the sac 130 proximal to the spike 123 is aluminum foil, PET film, PP film, or LDPE film. In other embodiments, the material of the sac 130 on the side near the spike 123 is a composite film of plastic film and aluminum foil. In some embodiments, the thickness of the sac 130 on the side near the spike 123 is 50 μm to 200 μm; the material of the side of the sac 130 away from the spike 123 is at least one of PP, HDPE, PVC and PET. The liquid bag 130 is made of the above materials (at least one of PP, HDPE, PVC and PET) through a blow molding or injection molding process to obtain a dome-shaped shell structure. In some embodiments, the thickness of the sac 130 on the side away from the spike 123 is 75 μm to 200 μm.

When the sac 130 is placed in the sac cavity 121, the sac 130 and the spike 123 are spaced apart by 1mm to 10 mm. For example 2mm, 5mm or 8 mm. The spaced distance between the sac 130 and the spike 123 means a distance from the nearest of the spike 123 to the sac 130 to a side of the sac 130 adjacent to the spike 123. It will be appreciated that the spacing between the sac 130 and the spike 123 when the sac 130 is placed in the sac chamber 121 may be adjusted depending on the material of the sac 130. If the material of the sac 130 is easily deformed by being pressed and easily approaches the spike 123, the distance between the sac 130 and the spike 123 may be set to be longer; if the material of the sac 130 is not easily deformed by being pressed and is not easily moved toward the spike 123, the interval between the sac 130 and the spike 123 may be set to be shorter. By the arrangement, the microfluidic chip 10 is not easy to scrap in the transportation and storage processes, and is easy to puncture in use.

Specifically, the chip body 110 is an important component of the POCT product, and the chip body 110 includes a reaction chamber and a fluid channel communicating with the reaction chamber. The chip body 110 is provided with a liquid inlet 111. The loading port 111 is communicated with a reaction chamber on the chip body 110. In the detection, a reagent for detection enters the chip body 110 from the inlet 111.

In some embodiments, a plurality of reaction chambers are disposed on the chip body 110, and the plurality of reaction chambers are communicated through a fluid flow channel. At least some of the reaction chambers have respective liquid inlets 111, and the liquid bag storage member 120 also has liquid bag cavities 121 and liquid outlets 122 corresponding to the reaction chambers. In the illustrated embodiment, the chip body 110 has six spaced inlet ports 111, and the fluid reservoir 120 also has six fluid chambers 121 and six outlet ports 122. In the illustrated embodiment, one bag chamber 121 corresponds to one liquid outlet 122, and one liquid outlet 122 corresponds to one liquid inlet 111. It is understood that in other embodiments, one liquid bag cavity 121 may correspond to a plurality of liquid outlets 122. The plurality of or one outlet ports 122 correspond to the one or more inlet ports 111. The number of inlet ports 111 and outlet ports 122 can be adjusted according to the actual situation. Of course, in some embodiments, there may be only one reaction chamber on the chip. At this time, there is one corresponding to the sac chamber 121 of the sac storage member 120. It is understood that the sizes of the inlet 111 and the outlet 122 are not particularly limited, and may be adjusted according to the actual conditions (such as the flow rate/flow rate of the liquid reagent).

In the illustrated embodiment, the chip body 110 has a disk shape. It is understood that in other embodiments, the shape of the chip body 110 is not limited to the disc shape, but may be any other shape. For example, a square block shape or an oval shape. In some embodiments, the material of the chip body 110 is selected from at least one of Polydimethylsiloxane (PDMS), polyurethane, epoxy, Polymethylmethacrylate (PMMA), Polycarbonate (PC), cyclic olefin copolymer (COC/COP), Polystyrene (PS), Polyethylene (PE), polypropylene (PP), and fluoroplastic. It is to be understood that the material of the chip body 110 is not limited to the above, but may be other materials.

Referring to fig. 3 to 5, the sac storage 120 is used to store a sac 130 filled with a liquid reagent. Specifically, a sac cavity 121 is disposed on the sac storage member 120, and the sac cavity 121 is used for placing the sac 130. In the illustrated embodiment, the reservoir storage member 120 is disc-shaped. It is understood that in other embodiments, the shape of the sac storing member 120 is not limited to the above, and may be adjusted according to actual needs. In the illustrated embodiment, the number of sac chambers 121 is six. It is understood that in other embodiments, the number of sac chambers 121 is not limited to the above, and may be adjusted according to actual conditions. Of course, the shape of the sac chamber 121 is not limited, but is matched to the sac 130. For example, in the illustrated embodiment, the sac 130 has a substantially hemispherical shape, and the sac chamber 121 has a substantially cylindrical shape with a bottom portion recessed toward the chip body 110.

In some embodiments, the material of the sac storage member 120 is a rigid material. The protection of the sac 130 in the sac chamber 121 is utilized by employing a rigid sac storage member 120.

In some embodiments, the bottom of the sac chamber 121 is funnel-shaped, the spike 123 is located on the bottom, and the liquid outlet 122 is located at the bottom closest to the chip body 110. The bottom of the liquid bag 130 is arranged in a funnel shape, so that a certain interval is formed between the sharp piercing member 123 and the liquid bag 130, and the liquid bag 130 can be pierced by the sharp piercing member 123 only under certain extrusion force instead of being slightly extruded, so that the rejection rate of the chip is reduced. In addition, the funnel-shaped bottom of the sac 130 facilitates the flow of the liquid reagent in the sac 130 to the chip body 110, so that the liquid reagent is not easily wasted. It is understood that in other embodiments, the bottom of the sac chamber 121 is not limited to a funnel shape, but may be other curved surfaces recessed toward the chip body 110.

In some embodiments, spike 123 is tapered or needle-shaped. In an alternative specific example, the spike 123 is conical or trigonal. After the spike 123 pierces the sealing film of the sac 130, the opening of the sealing film pierced becomes larger as the piercing depth increases. In some embodiments, the number of the spike members 123 is plural, and the plural spike members 123 are arranged around the liquid outlet 122 at intervals. Further, the plurality of spike members 123 are equidistant from the center of the liquid outlet 122. In the illustrated embodiment, the number of the spike members 123 is three, three spike members 123 are arranged around the liquid outlet 122 at intervals, and the distances from the three spike members 123 to the center of the liquid outlet 122 are equal. It is understood that in other embodiments, the number of spikes 123 is not limited to three, but may be other numbers, such as one, two, five, or six, etc.

Referring to fig. 6, in some embodiments, the spike 123 further comprises a drainage groove 124. Drainage channel 124 communicates with liquid outlet 122. Drainage channel 124 serves to direct liquid in bladder 130 toward outlet opening 122. In the illustrated embodiment, drainage groove 124 is located on the side of spike 123 adjacent to liquid outlet 122. It will be appreciated that in other embodiments, the location of the drainage slots 124 is not limited to that described above, and may be located elsewhere on the spike 123. For example on the side of the spike 123 remote from the exit orifice 122.

Referring to fig. 5, in some embodiments, the microfluidic chip 10 further includes a sealing member 125. The sealing member 125 is located between the sac storage member 120 and the chip body 110, and the sealing member 125 serves to seal a gap between the sac storage member 120 and the chip body 110. The sealing member 125 has a liquid passage 126, and the liquid outlet 122 communicates with the liquid inlet 111 through the liquid passage 126. Specifically, the liquid bag storage member 120 has a liquid outlet channel, one end of which is communicated with the liquid outlet 122, and the other end is communicated with the liquid inlet 111. The sealing member 125 is located in the liquid outlet channel, the outer sidewall of the sealing member 125 is connected with the sidewall of the liquid outlet channel in a sealing manner, and the sealing member 125 is connected with the chip body 110 in a sealing manner near the outer wall of the chip body 110. At this time, the liquid reagent flowing out of the liquid outlet 122 enters the liquid inlet 111 through the liquid passage 126. Optionally, the seal 125 is an elastomeric seal 125. In an alternative specific example, the material of the sealing member 125 is TPU, silicone rubber, or resin. It is understood that the material of the sealing member 125 is not limited to the above, but may be other materials that may be used for sealing.

It is understood that in some embodiments, the seal 125 may be omitted. At this time, a gap between the sac storage member 120 and the chip body 110, through which the liquid reagent does not flow into the chip body 110, may be sealed in another manner. For example, the sealant is used to seal the gap between the fluid bag storage member 120 and the chip body 110, which is not for the liquid reagent to flow into the chip body 110.

In some embodiments, the microfluidic chip 10 further comprises a sac 130. The sac 130 is used to hold a liquid reagent. Types of liquid reagents include, but are not limited to, at least one of buffer solutions (ionic solutions, surfactant mixtures), reaction solutions (antigen/antibody dilutions, protein dilutions, magnetic particle dilutions, luminescent/fluorescent reagent dilutions, nucleic acid dilutions, molecular/protein bioprobe dilutions, etc.), and wash solutions (surfactant mixtures, detergents, etc.). In the illustrated embodiment, the number of fluid bladders 130 is six. It is understood that, in other embodiments, the number of the liquid bags 130 is not limited to the above, and may be adjusted according to actual situations.

In some embodiments, the microfluidic chip 10 includes a protection member 140. The protection member 140 is used for reducing the extrusion of the external force on the liquid bag 130 during the transportation and/or storage process, avoiding the rejection of the microfluidic chip 10 due to the early puncture of the liquid bag 130 caused by the extrusion of the external force on the liquid bag 130 during the transportation and/or storage process, and further reducing the rejection rate of the microfluidic chip 10. The protection member 140 covers a side of the sac storage member 120 away from the chip body 110 and is fixedly connected to the chip body 110, and the protection member 140 is provided with a space-avoiding hole 141 corresponding to the sac chamber 121. The number of the avoiding holes 141 corresponds to the number of the liquid bags 130. In the illustrated embodiment, the number of the clearance holes 141 is six. When the microfluidic chip 10 is used, the liquid sac 130 is pressed through the position-avoiding hole 141, so that the liquid sac 130 is contacted with the spike 123 to be punctured. Herein, the manner of the fixed connection is not particularly limited unless otherwise specified. For example, the connection may be a detachable fixed connection such as a screw connection or a snap connection, or a non-detachable fixed connection such as an adhesive connection, a welding connection, a riveting connection, or an interference fit connection.

In the illustrated embodiment, the protector 140 has a disk shape. It is understood that in other embodiments, the shape of the protection member 140 is not limited to the above, and may be adjusted according to actual conditions.

In the illustrated embodiment, the clearance hole 141 is a through hole opened in the axial direction of the protector 140. The liquid pocket 130 is pressed by applying a force to the axial direction of the protection member 140 through the relief hole 141 such that the liquid pocket 130 is adjacent to the spike member 123 and is punctured by the spike member 123. It is understood that, in other embodiments, the position-avoiding hole 141 may also be opened in the radial direction of the protection member 140. At this time, the fluid bladder 130 is pressed by applying a force to the axial direction of the protection member 140 through the radially-directed relief hole 141, so that the fluid bladder 130 approaches the spike member 123 and is pierced by the spike member 123.

In some embodiments, the microfluidic chip 10 further comprises an indicator 150. Indicator 150 is used to indicate whether sac 130 is broken or not or is being squeezed by an external force. Specifically, the indicator 150 is located between the sac storage member 120 and the protection member 140, and the material of the indicator 150 is a material that is easily deformed by a force. At this time, the protection member 140 is a transparent protection member 140; and/or, the material of the sac storage member 120 and the material of the chip body 110 are both transparent materials. The parts (the protection member 140) positioned on the indicator 150 and the parts (the sac storage member 120 and the chip body 110) positioned under the indicator 150 are made of transparent materials, so that whether the indicator 150 is broken or deformed can be observed from above or below the indicator 150, and whether the sac 130 is squeezed by external force or broken can be judged. On the other hand, whether the test is completed or not can also be confirmed by the indicator 150. For example, upon assembly, indicator 150 is placed over the reagent for which the test is completed, and no indicator 150 is present on the reagent not taking part in the test.

Optionally, the material of the indicator 150 is a plastic film, aluminum foil, tin foil, or paper. In an alternative specific example, the material of the indicator 150 is at least one of a polyester film (PET), a polypropylene film (PP), and a polyethylene film (PE). In another alternative specific example, the material of the indicator 150 is one of soft/hard label paper with a dotted line impression, printing paper, and composite paper. It is understood that the material of the indicator 150 is not limited to the above, but may be other materials that are easily deformed by force.

In some embodiments, the microfluidic chip 10 further includes a first fixing member 160, and the sac storage member 120 and the chip body 110 are fixedly connected by the first fixing member 160. In the illustrated embodiment, the first fixing member 160 penetrates the protection member 140, the indicator 150 and the sac storage member 120, for fixedly connecting the protection member 140, the indicator 150 and the sac storage member 120 with the chip body 110. It is understood that the securing connection between the protection member 140 and the indicator member 150 and the fluid bag storage member 120 may be made by the first securing member 160, or may be made by other means. Optionally, the material of the first fixing member 160 is a thermoplastic material. The use of thermoplastic materials may facilitate the organization of the microfluidic chip 10. In the illustrated embodiment, the microfluidic chip 10 further includes a second fixing member 170, and the protection member 140 and the indicator 150 are fixedly connected to the sac storage member 120 through the second fixing member 170. Specifically, the second fixing member 170 penetrates the protection member 140 and the indicator 150 and is fixedly coupled to the sac storage member 120.

In some embodiments, the microfluidic chip 10 further has a positioning hole 180. When the microfluidic chip 10 is assembled or the microfluidic chip 10 is used, the positioning hole 180 is used for positioning, so that the assembly and the use are convenient.

In some embodiments, the microfluidic chip 10 includes a chip body 110 and a sac storage 120 on the chip body 110. That is, the microfluidic chip 10 at this time is a microfluidic chip 10 that can be loaded with the liquid bladder 130, which is formed by assembling the chip main body 110 and the liquid bladder storage member 120. The liquid bag 130 is freely selected according to specific requirements and then assembled with the microfluidic chip 10 capable of containing the liquid bag 130, so that the microfluidic chip 10 capable of being directly used is formed.

In some embodiments, the microfluidic chip 10 includes a chip body 110, a sac storage 120 located on the chip body 110, a sac 130 located in the sac storage 120, and a protection member 140 located on a side of the sac storage 120 away from the chip body 110, wherein the chip body 110 is fixedly connected to the sac storage 120 and the protection member 140. The microfluidic chip 10 in this case is a directly usable microfluidic chip 10. In the illustrated embodiment, the microfluidic chip 10 includes a chip body 110, a sac storage member 120 located on the chip body 110, a sac 130 located in the sac storage member 120, a protection member 140 located on a side of the sac storage member 120 away from the chip body 110, and an indicator member 150 located between the protection member 140 and the sac storage member 120, the chip body 110, the sac storage member 120, the protection member 140, and the indicator member 150 are fixedly connected by a first fixing member 160, and the sac storage member 120, the protection member 140, and the indicator member 150 are further fixedly connected by a second fixing member 170.

In some embodiments, the thickness of the microfluidic chip 10 is 10mm to 20 mm. Herein, the thickness of the microfluidic chip 10 refers to the axial length of the microfluidic chip 10, i.e., the distance from the side of the chip body 110 away from the fluid bag storage member 120 to the side of the protection member 140 away from the indicator member 150. Of course, the absence of the protective member 140 and the indicator 150 refers to the distance from the side of the chip body 110 away from the sac storage member 120 to the side of the sac storage member 120 away from the chip body 110. It is to be understood that the thickness of the microfluidic chip 10 is not limited to the above, and can be adjusted according to actual conditions.

The microfluidic chip 10 has at least the following advantages:

(1) when the liquid bag 130 is placed in the liquid bag cavity 121 and is not used, the liquid bag 130 is not punctured when the liquid bag 130 is subjected to a squeezing force not exceeding a preset value (a squeezing force applied in a general transportation or storage process) through the interval between the liquid bag 130 and the sharp piercing member 123, and the squeezing force exceeding the preset value can be punctured, so that the rejection rate of the microfluidic chip 10 is reduced.

(2) Through the arrangement of the spine member 123, after the liquid bags 130 are subjected to the extrusion force with the same magnitude, the sizes of the openings of the liquid bags 130 are basically the same, so that the flow rate and the flow resistance of the reagent flowing out of each liquid bag 130 are basically the same, the detection repeatability, the stability and the consistency of the microfluidic chip 10 can be improved, and the difference of the POCT test process is reduced. Moreover, the reagent drainage driving can be completed by means of manual operation or an external equipment mechanism, and the operation is simple; the design of the drainage groove 124 and the sealing member 125 greatly improves the final utilization efficiency of the liquid and reduces the loss and dead volume of the liquid in the flow transfer process.

(3) The multiple bonding assembly processes with the chip are suitable, the assembly position is outside the chip functional reaction area, the fluid design and the reaction flow design in the chip are not influenced, the reagent introduction can be realized only by arranging an interface on the upper surface of the chip and corresponding to a liquid injection inlet at the bottom of the device, and the modular design can be matched with chips with different detection requirements and different structural designs.

(4) The design of the plurality of liquid sacs 130 and the use of the liquid sacs 130 with different colors can distinguish different reagents, so that the microfluidic chip 10 has wide detection application and can be used as a modularized chip; and, multiple reagent can freely match, and the compatible chamber of reagent, and difficult confusion when the reagent kind is more.

Referring to fig. 7, an embodiment of the present invention further provides an assembling method of the microfluidic chip 10, in which the microfluidic chip 10 is prepared by fixing the chip body 110, the pouch storage member 120 in which the pouch 130 is disposed, the indicator 150, and the protector 140 by riveting. Specifically, the assembling method includes step S100 and step S200. Specifically, the method comprises the following steps:

step S100: the liquid bag storage member 120 is inserted into the first fixing column 161 of the chip main body 110, and the sealing member 125 seals the gap between the liquid outlet 122 and the liquid inlet 111, wherein the first fixing column 161 is fixed on the side of the chip main body 110 having the liquid inlet 111.

Step S200: the sac 130 is placed in the sac chamber 121 of the sac storage 120, and then the indicator 150 and the protector 140 are sequentially fitted over the second fixing post 171 of the indicator 150 and the first fixing post 161 of the chip body 110, and the chip body 110, the sac storage 120, the indicator 150, and the protector 140 are fixed by riveting.

In one embodiment, the riveting is a combination of hot air and cold riveting. Specifically, the first and second fixing posts 161, 171 are both thermoplastic materials. The hot air is heated and/or heated from one ends of the first and second fixing posts 161 and 171 near the protector 140, and when it is melted or softened, the first and second fixing posts 161 and 171 are pressed down using a cold jig to form rivets after a preset heating time, thereby fixing the chip main body 110, the sac storage 120, the indicator 150, and the protector 140.

In one of the embodiments, the riveting is ultrasonic riveting. Specifically, the first and second fixing posts 161, 171 are both thermoplastic materials. The first fixing column and the second fixing column 171 are heated by ultrasonic high-frequency vibration, and the ultrasonic pressure head melts the fixing columns to form the rivet. Compared with other riveting methods, the ultrasonic heating is very quick and the period is short. A proper staking design requires a small initial contact between the ultrasonic ram and the anchor post to produce a rapid heating effect. In the ultrasonic riveting process, high-amplitude vibration and the deceleration and descending of the ultrasonic pressure head are adopted, so that the stud is melted and flows, and the ultrasonic pressure head is filled to form a rivet cap, so that the chip body 110 is fixed with the liquid bag storage member 120, the indicating member 150 and the protecting member 140.

In one embodiment, the rivets are infrared or laser rivets. Specifically, similar to the ultrasonic caulking, only the way of causing the first fixing post 161 and the second fixing post 171 to melt is by infrared heating or laser heating.

In the assembly method of the above embodiment, the chip body 110 is fixed to the sac storage member 120, the indicator 150, and the protector 140 by caulking. It is understood that in other embodiments, the fixing manner of the chip body 110 and the sac storing member 120, the indicator 150 and the protection member 140 is not limited to riveting, and may be other fixing manners, such as gluing, ultrasonic welding, laser welding, and the like. The specific assembly process may be determined according to the actual microfluidic chip 10 requirements. For example, if the microfluidic chip 10 has a limited pressure-bearing capacity due to its low thickness or structural design factors, the use of riveting is not suitable, and the use of adhesive or ultrasonic welding is more suitable. For another example, if the storage reagent itself is not resistant to high temperature, the riveting, ultrasonic and laser welding processes are not suitable (the thermal effect is not controllable, the stability and performance of the reagent are affected, and even the reagent is disabled), and at this time, the normal temperature operation process, i.e. the gluing process, is more suitable.

The assembly method of the microfluidic chip 10 is simple, convenient and easy, and is beneficial to industrial production.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, so as to understand the technical solutions of the present invention specifically and in detail, but not to be understood as the limitation of the protection scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. It should be understood that the technical solutions obtained by logical analysis, reasoning or limited experiments based on the technical solutions provided by the present invention are all within the protection scope of the appended claims of the present invention. Therefore, the protection scope of the present invention should be subject to the content of the appended claims, and the description and the drawings can be used for explaining the content of the claims.

Claims

1. A microfluidic chip, comprising:

2. The microfluidic chip according to claim 1, wherein the spike member has a drainage groove, and the drainage groove is in communication with the liquid outlet.

3. The microfluidic chip according to claim 1, wherein the spike has a conical shape or a needle shape.

4. The microfluidic chip according to any one of claims 1 to 3, wherein the microfluidic chip comprises a protection member, the protection member is connected to the chip body and covers the liquid sac storage member, and the protection member is provided with a liquid sac avoiding hole corresponding to the liquid sac cavity.

5. The microfluidic chip according to claim 4, further comprising an indicator located between the capsule storage member and the protection member, wherein the indicator is made of a material that is easily deformable under a force; the protective piece is a transparent protective piece, and/or the material of the liquid sac storage piece and the material of the chip main body are both transparent materials.

6. The microfluidic chip according to claim 5, wherein the material of the indicator is plastic film, aluminum foil, tin foil or paper.

7. The microfluidic chip according to claim 1, further comprising a first fixing member for fixedly connecting the sac storage member and the chip body.

8. The microfluidic chip according to any one of claims 5 to 7, further comprising a second fixing member for fixedly connecting the protection member and the fluid bag storage member.

9. The microfluidic chip according to claim 4, further comprising a sealing member located between the fluid bag storage member and the chip body, the sealing member being configured to seal a gap between the fluid bag storage member and the chip body, the sealing member having a liquid channel, wherein the liquid outlet is in communication with the liquid inlet via the liquid channel.

10. The microfluidic chip according to any one of claims 1 to 3, 5 to 7 and 9, wherein the bottom of the liquid bag cavity is funnel-shaped, the spike is located on the bottom, and the liquid outlet is located at the bottom closest to the chip body.