CN113151141B - Culture method of strain for sewage treatment and culture medium thereof - Google Patents
Culture method of strain for sewage treatment and culture medium thereof Download PDFInfo
- Publication number
- CN113151141B CN113151141B CN202110610762.3A CN202110610762A CN113151141B CN 113151141 B CN113151141 B CN 113151141B CN 202110610762 A CN202110610762 A CN 202110610762A CN 113151141 B CN113151141 B CN 113151141B
- Authority
- CN
- China
- Prior art keywords
- culture
- sewage treatment
- strain
- culturing
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture method of a strain for sewage treatment and a culture medium thereof, belonging to the technical field of microbial culture. The culture medium comprises, by weight, 0.6-0.8 part of organic carbon source, 0.44-0.6 part of organic nitrogen source, 0.8-1 part of inorganic carbon source, 0.11-0.116 part of inorganic nitrogen source, 0.1246-0.249 part of metal ion source, 0.106-0.17 part of potential regulator and the balance of water. The culture medium is suitable for culturing strains for sewage treatment, can effectively improve the viable count of the cultured strains, and can obtain good sewage treatment effect under the condition of greatly reducing the use amount of bacterial powder when sewage treatment is carried out, thereby reducing the sewage treatment cost.
Description
Technical Field
The invention relates to the technical field of microorganism culture, in particular to a culture method and a culture medium of a strain for sewage treatment.
Background
Along with the rapid development of industry and the improvement of living standard of people, a large amount of nitrogen-containing substances enter water body, the water body environment is seriously polluted, the nitrogen content in water has a tendency of gradually increasing, and ammonia nitrogen becomes an important content of sewage treatment work in China in the aspect of water body pollutant control.
The sewage treatment method is mainly divided into two types of physical and chemical denitrification and biological denitrification, and the physical and chemical method is adopted to treat ammonia nitrogen sewage, so that the defects of secondary pollution to the environment, high treatment cost and the like are easily caused, and the biological denitrification process is mainly adopted for treating the nitrogen-containing sewage at present.
The biological denitrification technology is characterized in that ammonia nitrogen in sewage is converted into nitrite nitrogen and nitrate nitrogen under the action of nitrifying bacteria, and then nitrite nitrogen and nitrate nitrogen are reduced and converted into nitrogen by denitrifying bacteria, so that the aim of removal is achieved, and the biological denitrification technology is favored because of high efficiency and safety.
Biological denitrification of sewage is carried out by most enterprises mainly by purchasing existing composite bacterial powder for sewage treatment, and as the biological denitrification process involves a plurality of bacterial species and the optimal growth conditions among different bacterial species are inconsistent, a complex sewage treatment process, related equipment and sites are required, such as a multistage treatment pool is used, dissolved oxygen, pH, carbon sources and the like are required to be monitored at any time, a great amount of added bacterial powder is adopted by some enterprises to achieve the aim of removing ammonia nitrogen from sewage, and the cost is extremely high.
In view of this, the present invention has been made.
Disclosure of Invention
One of the purposes of the invention is to provide a culture medium which can effectively increase the number of viable bacteria after bacterial culture and reduce the sewage treatment cost.
The second object of the invention comprises providing an application of the culture medium, namely, the culture medium is used for culturing bacteria for sewage treatment.
The third object of the present invention is to provide a method for culturing a strain for sewage treatment, wherein the strain for sewage treatment is cultured using the above-mentioned culture medium during the culturing process.
The application can be realized as follows:
in a first aspect, the present application provides a culture medium comprising, in parts by weight, 0.6 to 0.8 part of an organic carbon source, 0.44 to 0.6 part of an organic nitrogen source, 0.8 to 1 part of an inorganic carbon source, 0.11 to 0.116 part of an inorganic nitrogen source, 0.1246 to 0.249 part of a metal ion source, 0.106 to 0.17 part of a potential regulator, and the balance being water per 100 parts of a formulation raw material of the culture medium.
In an alternative embodiment, the organic carbon source is glucose, or the inorganic carbon source comprises at least one of sodium bicarbonate and potassium bicarbonate.
In an alternative embodiment, the organic nitrogen source comprises at least one of yeast extract, peptone, and urea. In a preferred embodiment, the organic nitrogen source comprises both yeast extract, peptone and urea. In a more preferred embodiment, the organic nitrogen source comprises, by weight of the same, 0.16-0.2 parts yeast extract, 0.16-0.2 parts peptone and 0.1-0.16 parts urea.
In an alternative embodiment, the organic nitrogen source further comprises at least one of cysteine and methionine. In a preferred embodiment, the organic nitrogen source also includes both cysteine and methionine. In a more preferred embodiment, the organic nitrogen source also includes both 0.01 to 0.02 parts cysteine and 0.01 to 0.02 parts methionine, based on the same parts by weight.
In an alternative embodiment, the inorganic nitrogen source includes at least one of ammonium sulfate, sodium nitrate, and potassium nitrate. In a preferred embodiment, the inorganic nitrogen source comprises both ammonium sulfate and sodium nitrate. In a more preferred embodiment, the inorganic nitrogen source comprises, by the same parts by weight, 0.1 to 0.16 parts ammonium sulfate and 0.01 to 0.016 parts sodium nitrate.
In an alternative embodiment, the metal ion source includes at least one of a magnesium source, a manganese source, a calcium source, a zinc source, a cobalt source, an iron source, and a molybdenum source.
In an alternative embodiment, the magnesium source is magnesium sulfate; or the manganese source is manganese sulfate; or, the calcium source is calcium chloride; or, the zinc source is zinc chloride; or, the cobalt source is cobalt chloride; or, the iron source is ferrous sulfate; or, the molybdenum source is ammonium molybdate. In a preferred embodiment, the metal ion source includes magnesium sulfate, manganese sulfate, calcium chloride, zinc chloride, cobalt chloride, ferrous sulfate, and ammonium molybdate. In a more preferred embodiment, the metal ion source comprises, by the same parts by weight, 0.1 to 0.2 parts magnesium sulfate, 0.0006 to 0.001 parts manganese sulfate, 0.02 to 0.04 parts calcium chloride, 0.001 to 0.002 parts zinc chloride, 0.001 to 0.002 parts cobalt chloride, 0.001 to 0.002 parts ferrous sulfate, and 0.001 to 0.002 parts ammonium molybdate.
In an alternative embodiment, the potential modifier comprises at least one of sodium thiosulfate and dipotassium hydrogen phosphate. In a preferred embodiment, the potential modifier comprises both sodium thiosulfate and dipotassium hydrogen phosphate. In a more preferred embodiment, the potential modifier comprises, by the same parts by weight, 0.1 to 0.16 part sodium thiosulfate and 0.006 to 0.01 part dipotassium hydrogen phosphate.
In a second aspect, the present application provides the use of a culture medium according to any of the preceding embodiments, for example for culturing a strain for sewage treatment.
In an alternative embodiment, the sewage treatment bacterial species include at least one species of the genera nitrifying bacteria, denitrifying bacteria, bacillus and pseudomonas.
In an alternative embodiment, the sewage treatment bacterial species contain nitrifying bacteria, denitrifying bacteria, bacillus and pseudomonas simultaneously.
In a third aspect, the present application provides a method for culturing a strain for sewage treatment, comprising the steps of: inoculating a culture to be cultured containing a strain for sewage treatment in a first vessel containing the medium according to any one of the above embodiments for first cultivation.
In an alternative embodiment, the culturing conditions during the first culturing include:
in the process of culturing for 0-6h, the stirring rotation speed is 45-55r/min, the ventilation ratio is 0.78-0.86, and the temperature is 22-24 ℃; in the process of culturing for 6-12h, the stirring rotation speed is 140-160r/min, the ventilation ratio is 1.20-1.30, and the temperature is 24-26 ℃; in the process of culturing for 12-18h, the stirring rotation speed is 200-220r/min, the ventilation ratio is 1.62-1.74, and the temperature is 30-32 ℃; in the process of culturing for 18-24h, the stirring rotation speed is 80-90r/min, the ventilation ratio is 0.36-0.42, and the temperature is 36-38 ℃.
In an alternative embodiment, the inoculum size of the culture to be cultivated is 2-3wt% of the medium during the first cultivation.
In an alternative embodiment, the alkaline solution is periodically replenished during the first cultivation to a pH of not less than 6.8 and not more than 8.0 in the first container.
In an alternative embodiment, the lye is an aqueous sodium hydroxide solution having a concentration of 8 to 12 wt%.
In an alternative embodiment, the sewage treatment bacterial species include at least one species of the genera nitrifying bacteria, denitrifying bacteria, bacillus and pseudomonas.
In an alternative embodiment, the sewage treatment bacterial species contain nitrifying bacteria, denitrifying bacteria, bacillus and pseudomonas simultaneously.
In an alternative embodiment, further comprising transferring the material after the first culturing to a second vessel containing a medium according to any of the preceding embodiments for expansion culturing.
In an alternative embodiment, the culture conditions during the expansion culture include: in the process of culturing for 0-6h, the stirring rotation speed is 45-55r/min, the ventilation ratio is 0.78-0.86, and the temperature is 22-24 ℃; in the process of culturing for 6-12h, the stirring rotation speed is 140-160r/min, the ventilation ratio is 1.20-1.30, and the temperature is 24-26 ℃; in the process of culturing for 12-18h, the stirring rotation speed is 200-220r/min, the ventilation ratio is 1.62-1.74, and the temperature is 30-32 ℃; in the process of culturing for 18-24h, the stirring rotation speed is 80-90r/min, the ventilation ratio is 0.36-0.42, and the temperature is 36-38 ℃.
In an alternative embodiment, the volume of the second container is 10-20 times the volume of the first container.
In an alternative embodiment, the transfer is performed using a sterilized transfer line.
The beneficial effects of this application include:
the application provides a culture medium, through carrying out the compounding with organic carbon source, inorganic carbon source, organic nitrogen source, inorganic nitrogen source, metal ion source and potential regulator according to specific ratio for the required nutrient substance of bacterial can not only be provided to the resulting culture medium, but also good potential and osmotic pressure condition can be maintained simultaneously, thereby effectively improve the viable count after the bacterial is cultivateed, when carrying out sewage treatment, can obtain good sewage treatment effect under the condition of greatly reduced fungus powder's use amount, reduce sewage treatment cost.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
The method for culturing the strain for sewage treatment and the culture medium thereof provided by the application are specifically described below.
The application provides a culture medium, which comprises, by weight, 0.6-0.8 part of an organic carbon source, 0.44-0.6 part of an organic nitrogen source, 0.8-1 part of an inorganic carbon source, 0.11-0.116 part of an inorganic nitrogen source, 0.1246-0.249 part of a metal ion source, 0.106-0.17 part of a potential regulator and the balance of water.
The organic carbon source may be 0.6 part, 0.65 part, 0.7 part, 0.75 part, 0.8 part, or the like, or any other part number in the range of 0.6 to 0.8.
The organic nitrogen source may be 0.44 parts, 0.45 parts, 0.5 parts, 0.55 parts, 0.6 parts, etc., and may be any other number in the range of 0.44 to 0.6.
The inorganic carbon source may be 0.8 part, 0.85 part, 0.9 part, 0.95 part, 1 part, or the like, or any other part number in the range of 0.8 to 1.
The inorganic nitrogen source may be 0.11 part, 0.112 part, 0.115 part, 0.116 part, or the like, or any other part value in the range of 0.11 to 0.116.
The metal ion source may be 0.1246 parts, 0.15 parts, 0.18 parts, 0.2 parts, 0.22 parts, 0.249 parts, or the like, or any other number in the range of 0.1246 to 0.249.
The potential regulator may be 0.106 parts, 0.12 parts, 0.15 parts or 0.17 parts, etc., or any other value in the range of 0.106 to 0.17.
Wherein the organic carbon source may be glucose.
The inorganic carbon source may include at least one of sodium bicarbonate and potassium bicarbonate.
The organic nitrogen source may include at least one of yeast extract, peptone, and urea.
In a preferred embodiment, the organic nitrogen source comprises both yeast extract, peptone and urea.
In a more preferred embodiment, the organic nitrogen source may include, by weight of the same, 0.16 to 0.2 part (e.g., 0.16 part, 0.17 part, 0.18 part, 0.19 part, or 0.2 part, etc.) of yeast extract, 0.16 to 0.2 part (e.g., 0.16 part, 0.17 part, 0.18 part, 0.19 part, or 0.2 part, etc.) of peptone, and 0.1 to 0.16 part (e.g., 0.1 part, 0.11 part, 0.12 part, 0.13 part, 0.14 part, 0.15 part, or 0.16 part, etc.) of urea. Wherein the yeast extract can be yeast extract powder or yeast extract.
In some preferred embodiments, the organic nitrogen source further comprises at least one of cysteine and methionine.
In a more preferred embodiment, both cysteine and methionine are included in the organic nitrogen source.
In a further preferred embodiment, the organic nitrogen source includes both 0.01 to 0.02 parts (e.g., 0.01 parts, 0.015 parts, or 0.02 parts, etc.) of cysteine and 0.01 to 0.02 parts (e.g., 0.01 parts, 0.015 parts, or 0.02 parts, etc.) of methionine, based on the same parts by weight.
The inorganic nitrogen source may include at least one of ammonium sulfate, sodium nitrate, and potassium nitrate.
In a preferred embodiment, the inorganic nitrogen source comprises both ammonium sulfate and sodium nitrate, or both ammonium sulfate and potassium nitrate.
In a more preferred embodiment, the inorganic nitrogen source comprises 0.1 to 0.16 parts (e.g., 0.1 part, 0.11 part, 0.12 part, 0.13 part, 0.14 part, 0.15 part, or 0.16 part, etc.) ammonium sulfate and 0.01 to 0.016 part (e.g., 0.01 part, 0.011 part, 0.012 part, 0.014 part, 0.015 part, or 0.016 part, etc.) sodium nitrate, or 0.1 to 0.16 part (e.g., 0.1 part, 0.11 part, 0.12 part, 0.13 part, 0.14 part, 0.15 part, or 0.16 part, etc.) ammonium sulfate and 0.01 to 0.016 part (e.g., 0.01 part, 0.011 part, 0.012 part, 0.013 part, 0.014 part, 0.015 part, or 0.016 part, etc.) potassium nitrate, on the same weight basis.
The metal ion source may include at least one of a magnesium source, a manganese source, a calcium source, a zinc source, a cobalt source, an iron source, and a molybdenum source.
In an alternative embodiment, the magnesium source is magnesium sulfate; or the manganese source is manganese sulfate; or, the calcium source is calcium chloride; or, the zinc source is zinc chloride; or, the cobalt source is cobalt chloride; or, the iron source is ferrous sulfate; or, the molybdenum source is ammonium molybdate.
In a preferred embodiment, the metal ion source includes magnesium sulfate, manganese sulfate, calcium chloride, zinc chloride, cobalt chloride, ferrous sulfate, and ammonium molybdate.
In a more preferred embodiment, the metal ion source comprises, by the same weight parts, 0.1 to 0.2 parts (e.g., 0.1 part, 0.15 part, or 0.2 part, etc.) magnesium sulfate, 0.0006 to 0.001 part (e.g., 0.0006 part, 0.0008 part, or 0.001 part, etc.) manganese sulfate, 0.02 to 0.04 part (e.g., 0.02 part, 0.025 part, 0.03 part, 0.035 part, or 0.04 part, etc.), 0.001 to 0.002 part (e.g., 0.001 part, 0.0015 part, or 0.002 part, etc.) zinc chloride, 0.001 to 0.002 part (e.g., 0.001 part, 0.0015 part, or 0.002 part, etc.) cobalt chloride, 0.001 to 0.002 part (e.g., 0.001 part, 0.0015 part, or 0.002 part, etc.) ferrous sulfate, and 0.001 to 0.002 part (e.001 part, 0.0015 part, 0.002 part, etc.) ammonium molybdate.
The potential regulator may include at least one of sodium thiosulfate and dipotassium hydrogen phosphate.
In a preferred embodiment, the potential modifier comprises both sodium thiosulfate and dipotassium hydrogen phosphate.
In a more preferred embodiment, the potential modifier comprises, by the same weight, 0.1 to 0.16 part (e.g., 0.1 part, 0.12 part, 0.15 part, or 0.16 part, etc.) sodium thiosulfate and 0.006 to 0.01 part (e.g., 0.006 part, 0.008 part, or 0.01 part, etc.) dipotassium hydrogen phosphate.
In addition to the yeast extract, peptone and urea described above as organic nitrogen sources, the yeast extract, peptone and urea described above also function to provide growth factors that promote the growth of bacteria. And urea is easier to gradually decompose into NH in the culture medium 3 ，NH 3 As an important nitrogen source of nitrifying bacteria, the concentration is too much and too littleIs beneficial to the growth of nitrifying bacteria, and NH can be continuously and gradually decomposed in the culture process by arranging urea with specific content 3 NH during the culture process for a longer period of time 3 Controlling the concentration range suitable for the growth of nitrifying bacteria.
Sodium bicarbonate serves to reduce the oxidation-reduction potential in addition to being an inorganic carbon source. It can reduce oxidation-reduction potential together with sodium thiosulfate, and has effects of promoting anaerobic bacteria growth and inhibiting aerobic bacteria growth. Most of aerobic bacteria have higher growth competition capability than anaerobic bacteria under the condition of oxygen, so that the overgrowth of the aerobic bacteria is easy to cause, the growth of the anaerobic bacteria is inhibited, and part of anaerobic bacteria can also be inhibited under the high oxidation-reduction potential, and the problem that the growth of the anaerobic bacteria is inhibited due to the overgrowth of the aerobic bacteria can be prevented by reducing the oxidation-reduction potential of the culture medium. Dipotassium hydrogen phosphate can not only play a role in maintaining potential difference and osmotic pressure, but also can provide phosphorus element.
Cysteine and methionine in the organic nitrogen source are used as sulfur-containing amino acids required for the growth of partial denitrifying bacteria in addition to the organic nitrogen source, and as amino acids, they are gradually decomposed into NH 3 Plays a role in providing nitrogen source for nitrifying bacteria.
On the contrary, the culture medium provided by the application is compounded with the organic carbon source, the inorganic carbon source, the organic nitrogen source, the inorganic nitrogen source, the metal ion source and the potential regulator according to a specific proportion, so that the obtained culture medium not only can provide nutrient substances required by strains, but also can maintain good potential and osmotic pressure conditions, thereby being beneficial to the growth and propagation of partial heterotrophic bacteria, being beneficial to improving the competitiveness of anaerobic bacteria, solving the problems that various strains are unbalanced in growth and even the growth of partial bacteria is completely inhibited due to different nutritional requirements, and effectively improving the viable count of the strains after culture.
Correspondingly, the application also provides application of the culture medium, such as application of the culture medium in culturing strain for sewage treatment.
In an alternative embodiment, the sewage treatment bacterial species include at least one species of the genera nitrifying bacteria, denitrifying bacteria, bacillus and pseudomonas.
It is worth to say that the common composite bacterial powder for sewage treatment in the market at present mainly consists of nitrifying bacteria, denitrifying bacteria, bacillus, pseudomonas and other bacteria, partial enzymes, polysaccharide and other bacteria, and the nutrient requirements and growth conditions of different bacteria are different, and the existing culture medium can not meet the culture requirements of each strain. The culture medium is particularly suitable for sewage treatment strains containing nitrifying bacteria, denitrifying bacteria, bacillus and pseudomonas, can effectively amplify each strain and has a high viable count, and can obtain good sewage treatment effect under the condition of greatly reducing the using amount of bacterial powder when sewage treatment is performed, so that the sewage treatment cost is reduced.
In addition, the application also provides a culture method of the strain for sewage treatment, which comprises the following steps: inoculating a culture to be cultured containing a strain for sewage treatment in a first vessel containing the medium according to any one of the above embodiments for first cultivation.
By way of reference, the first vessel may be a first fermenter (referred to herein as a "primary fermenter"). During the first cultivation, the inoculum size of the culture to be cultivated is controlled to 2-3wt% (e.g. 2wt%, 2.5wt% or 3wt% etc.) of the medium.
In the specific operation, water accounting for 40-50% of the volume of the fermentation tank can be added into the primary fermentation tank, then the raw materials of the culture medium provided by the application are added into the primary fermentation tank, the raw materials are stirred uniformly, water is added into the primary fermentation tank to fix the volume to 60-70% of the volume of the fermentation tank, and the primary fermentation tank is sterilized at 121 ℃ for 20 minutes. After sterilization, the initial pH is adjusted to 7.8-8.0, the temperature is 22-24 ℃, and the stirring is carried out for 45-55r/min. The culture to be cultivated is inoculated into a primary fermentation tank according to the inoculation amount for primary cultivation, and fermentation is carried out for 24 hours.
The culture conditions during the first culture include:
in the process of culturing for 0-6h, the stirring rotation speed is 45-55r/min (such as 45r/min, 50r/min or 55r/min, etc.), the ventilation ratio is 0.78-0.86 (such as 0.78, 0.8, 0.82, 0.85 or 0.86, etc.), and the temperature is 22-24deg.C (such as 22deg.C, 23deg.C or 24deg.C, etc.); in the process of culturing for 6-12h, the stirring rotation speed is 140-160r/min (such as 140r/min, 145r/min, 150r/min, 155r/min or 160r/min, etc.), the ventilation ratio is 1.20-1.30 (such as 1.2, 1.25 or 1.3, etc.), and the temperature is 24-26 ℃ (such as 24 ℃, 25 ℃ or 26 ℃); in the process of culturing for 12-18h, the stirring rotation speed is 200-220r/min (such as 200r/min, 205r/min, 210r/min, 215r/min or 220r/min, etc.), the ventilation ratio is 1.62-1.74 (such as 1.62, 1.65, 1.7 or 1.74, etc.), and the temperature is 30-32 ℃ (such as 30 ℃, 31 ℃ or 32 ℃ etc.); in the process of culturing for 18-24h, the stirring rotation speed is 80-90r/min (such as 80r/min, 85r/min or 90r/min, etc.), the ventilation ratio is 0.36-0.42 (such as 0.36, 0.38, 0.4 or 0.42, etc.), and the temperature is 36-38deg.C (such as 36deg.C, 37deg.C or 38deg.C, etc.).
In an alternative embodiment, lye is not periodically replenished during the first cultivation to bring the pH in the primary fermenter to not less than 6.8 and not more than 8.0. The lye may be an aqueous sodium hydroxide solution having a concentration of 8 to 12wt%, or alternatively, an aqueous potassium hydroxide solution having a corresponding concentration.
The culture to be cultured can be only a strain for sewage treatment, or can be composite bacterial powder for sewage treatment which contains the strain for sewage treatment and other components (such as enzyme, polysaccharide and the like). Wherein the sewage treatment bacterial species may include at least one species of the genus nitrifying bacteria, denitrifying bacteria, bacillus and pseudomonas, preferably contains nitrifying bacteria, denitrifying bacteria, bacillus and pseudomonas at the same time.
Further, the material after the first culture is transferred to a second container containing the culture medium provided herein for expansion culture.
By reference, the second vessel is a second fermenter (referred to herein as a "secondary fermenter"). The volume of the second container may be, for example, 10-20 times the volume of the first container.
During specific operation, the bacterial liquid in the primary fermentation tank is transferred to a secondary fermentation tank after sterilization, initial parameters are regulated, parameters such as ventilation, rotating speed, temperature and the like are regulated regularly in the culture process, and the culture is carried out for 24 hours. Wherein, the transferring can be performed by a sterilized transferring pipeline, the sterilizing can be performed by steam sterilization for 30-40min.
The culture conditions during the expansion culture include: in the process of culturing for 0-6h, the stirring rotation speed is 45-55r/min, the ventilation ratio is 0.78-0.86, and the temperature is 22-24 ℃; in the process of culturing for 6-12h, the stirring rotation speed is 140-160r/min, the ventilation ratio is 1.20-1.30, and the temperature is 24-26 ℃; in the process of culturing for 12-18h, the stirring rotation speed is 200-220r/min, the ventilation ratio is 1.62-1.74, and the temperature is 30-32 ℃; in the process of culturing for 18-24h, the stirring rotation speed is 80-90r/min, the ventilation ratio is 0.36-0.42, and the temperature is 26-38 ℃.
Preferably, the composition of the medium used for both cultures is the same as the culture conditions.
It is to be noted that the culture conditions set forth in the present application are set for the specific culture medium and the specific culture strain used in the present application:
the reasons why the conditions of the initial culture (0-6 h) were set to low temperature and low agitation in the present application include: the composite bacterial powder contains nitrifying bacteria and denitrifying bacteria, and meanwhile, the bacterial strain contains not only aerobic bacteria but also anaerobic bacteria. The most suitable growth temperature of the nitrifying bacteria is lower than that of the denitrifying bacteria, and the nitrifying bacteria is not high-temperature-resistant and is easy to inhibit, so that the growth speed of the denitrifying bacteria is reduced, and the situation that the number of the denitrifying bacteria in the middle and later stages is excessive and the denitrifying bacteria is difficult to inhibit is prevented; meanwhile, reasons for setting the low ventilation ratio include: in the middle fermentation stage of fast growth of thalli and higher dissolved oxygen, the growth speed of aerobe is far higher than that of anaerobe, and if the balance is adjusted by ventilation in the later stage when the concentration of aerobe is high, the aerobe can die, and the growth of the aerobe in the early stage can be inhibited and the growth of anaerobe can be promoted by setting the aeration to be low in the early stage.
The method gradually increases the aeration ratio, the stirring rotation speed and the temperature in the middle fermentation period (6-18 h), and is suitable for the growth of most bacteria, and the period is mainly the expansion culture stage of each strain.
The stirring rotation speed and the ventilation ratio are regulated down in the later fermentation period (18-24 h), the growth of the aerobic bacteria can be slightly inhibited, meanwhile, the growth of the anaerobic bacteria can be promoted, the temperature is continuously regulated up on the basis, and the growth of nitrifying bacteria can be inhibited, so that the relative balance of the quantity of the composite bacteria is achieved.
By the culture process, different strains such as aerobic bacteria, anaerobic bacteria, autotrophic bacteria, heterotrophic bacteria and the like can be grown together under the condition of keeping a relative balance, so that the effective viable count is amplified as much as possible under the condition of keeping the relative balance in number among the compound strains.
The features and capabilities of the present invention are described in further detail below in connection with the examples.
Example 1
The embodiment provides a method for culturing a strain for sewage treatment, which comprises the following steps:
A. first-stage culture
Adding 45% water of the volume of a fermentation tank into a primary fermentation tank (300L), adding culture medium raw materials (8 kg), uniformly stirring, adding water to a certain volume to 65% of the volume of the fermentation tank, sterilizing at 121 ℃ for 20 minutes, adjusting the initial pH to 8, the temperature to 22 ℃ and the stirring rotation speed to 50r/min, adding 2.5% of the weight of the culture medium into the culture medium, fermenting for 24 hours, controlling the pH to be not lower than 6.8 and not higher than 8.0 in the fermentation process by supplementing alkali (10 wt% sodium hydroxide aqueous solution), and adjusting ventilation volume, stirring rotation speed and temperature in different times.
Wherein each liter of the culture medium contains 8g of glucose, 1.8g of yeast extract powder, 1.8g of peptone, 1.6g of urea, 1.6g of ammonium sulfate, 0.16g of sodium nitrate, 10g of sodium bicarbonate, 1.2g of sodium thiosulfate, 0.08g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate, 0.006g of manganese sulfate, 0.2g of calcium chloride, 0.02g of zinc chloride, 0.02g of cobalt chloride, 0.02g of ferrous sulfate, 0.02g of ammonium molybdate, 0.2g of cysteine and 0.2g of methionine, and the balance of water.
The regulation and control of the aeration ratio, stirring rotation speed and temperature in the primary culture process are as follows:
0-6h: stirring speed is 50r/min, ventilation ratio is 0.83, and temperature is 22 ℃;
6-12h: stirring speed is 150r/min, ventilation ratio is 1.25, and temperature is 26 ℃;
12-18h: stirring speed is 200r/min, ventilation ratio is 1.67, and temperature is 32 ℃;
18-24h: the stirring speed was 80r/min, the aeration ratio was 0.42, and the temperature was 38 ℃.
B. Expansion culture
Adding water accounting for 40% of the volume of a fermentation tank into a secondary fermentation tank (3000L) with the volume being 10 times that of a primary fermentation tank, adding water to a constant volume to 60% of the volume of the fermentation tank after uniformly stirring, sterilizing at 115 ℃ for 15 minutes, adjusting the initial pH to 8 after sterilization, controlling the temperature to 22 ℃, stirring for 50r/min, transferring the culture medium of the primary fermentation tank into the secondary fermentation tank through a sterilized seed transfer pipeline, fermenting for 24 hours, controlling the pH to be not lower than 6.8 and not higher than 8.0 in the fermentation process through alkali supplementation, and adjusting the ventilation capacity, stirring rotation speed and temperature at different times.
Wherein, the sterilization mode of the seed transfer pipeline is that steam is introduced for 35 minutes; the culture medium raw materials, the alkali supplementing raw materials and the process parameter regulation and control used in the expansion culture process are the same as those in the primary culture process.
Example 2
This embodiment differs from embodiment 1 in that:
each liter of the culture medium contains 6g of glucose, 2g of yeast extract powder, 1.6g of peptone, 1.6g of urea, 1g of ammonium sulfate, 0.1g of sodium nitrate, 10g of sodium bicarbonate, 1g of sodium thiosulfate, 0.06g of dipotassium hydrogen phosphate, 2g of magnesium sulfate, 0.01g of manganese sulfate, 0.4g of calcium chloride, 0.01g of zinc chloride, 0.01g of cobalt chloride, 0.02g of ferrous sulfate, 0.01g of ammonium molybdate, 0.2g of cysteine, 0.2g of methionine and the balance of water.
Example 3
This embodiment differs from embodiment 1 in that:
each liter of the culture medium contains 8g of glucose, 1.6g of yeast extract powder, 2g of peptone, 1g of urea, 1.6g of ammonium sulfate, 0.16g of sodium nitrate, 8g of sodium bicarbonate, 1.6g of sodium thiosulfate, 0.1g of dipotassium hydrogen phosphate, 1g of magnesium sulfate, 0.006g of manganese sulfate, 0.2g of calcium chloride, 0.02g of zinc chloride, 0.02g of cobalt chloride, 0.01g of ferrous sulfate, 0.02g of ammonium molybdate, 0.1g of cysteine, 0.1g of methionine and the balance of water.
Example 4
This embodiment differs from embodiment 1 in that:
each liter of the culture medium contains 6.5g of glucose, 1.8g of yeast extract, 1.8g of peptone, 1.2g of urea, 1.2g of ammonium sulfate, 0.12g of potassium nitrate, 8.5g of potassium bicarbonate, 1.5g of sodium thiosulfate, 0.08g of dipotassium hydrogen phosphate, 1.4g of magnesium sulfate, 0.007g of manganese sulfate, 0.25g of calcium chloride, 0.018g of zinc chloride, 0.015g of cobalt chloride, 0.018g of ferrous sulfate, 0.015g of ammonium molybdate, 0.15g of cysteine and 0.18g of methionine, and the balance of water.
Example 5
This embodiment differs from embodiment 1 in that:
each liter of the culture medium contains 7.5g of glucose, 1.9g of yeast extract, 1.9g of peptone, 1.5g of urea, 1.5g of ammonium sulfate, 0.15g of potassium nitrate, 9.5g of potassium bicarbonate, 1.4g of sodium thiosulfate, 0.09g of dipotassium hydrogen phosphate, 1.8g of magnesium sulfate, 0.008g of manganese sulfate, 0.35g of calcium chloride, 0.015g of zinc chloride, 0.018g of cobalt chloride, 0.015g of ferrous sulfate, 0.015g of ammonium molybdate, 0.18g of cysteine and 0.15g of methionine, and the balance of water.
Example 6
This embodiment differs from embodiment 1 in that:
the regulation and control of the aeration ratio, stirring rotation speed and temperature in the two culture processes are as follows:
0-6h: the stirring speed is 45r/min, the ventilation ratio is 0.78, and the temperature is 23 ℃;
6-12h: the stirring speed is 140r/min, the ventilation ratio is 1.2, and the temperature is 24 ℃;
12-18h: stirring speed is 210r/min, ventilation ratio is 1.62, and temperature is 30 ℃;
18-24h: the stirring speed was 85r/min, the aeration ratio was 0.36, and the temperature was 36 ℃.
Example 7
This embodiment differs from embodiment 1 in that:
the regulation and control of the aeration ratio, stirring rotation speed and temperature in the two culture processes are as follows:
0-6h: the stirring speed is 55r/min, the ventilation ratio is 0.86, and the temperature is 24 ℃;
6-12h: stirring speed is 160r/min, ventilation ratio is 1.3, and temperature is 25 ℃;
12-18h: stirring speed is 220r/min, ventilation ratio is 1.74, and temperature is 31 ℃;
18-24h: the stirring speed was 90r/min, the aeration ratio was 0.40, and the temperature was 37 ℃.
Comparative example
Taking example 1 as an example, comparative examples 1 to 6 were set, wherein comparative examples 1 to 3 were different from example 1 only in the medium composition (water removal) (specifically as shown in Table 1), and the remaining culture conditions were the same as example 1. Comparative examples 4 to 6 are different from example 1 in that the culture process conditions are different (specifically, as shown in Table 2), and the remaining culture conditions are the same as example 1.
TABLE 1 Medium composition
That is, comparative example 1 differs from example 1 in that: the preparation raw materials of the culture medium do not contain ammonium thiosulfate, and the dosage of dipotassium hydrogen phosphate is different (but the total amount of sodium thiosulfate and dipotassium hydrogen phosphate 2 substances in the example 1 and the comparative example 1 is the same); comparative example 2 differs from example 1 in that: the preparation raw materials of the culture medium do not contain urea, and the dosages of the yeast extract, the peptone and the ammonium sulfate are different (but the total amount of 4 substances of urea, the yeast extract, the peptone and the ammonium sulfate in the example 1 is the same as that in the comparative example 2); comparative example 3 differs from example 1 in that: the cysteine and methionine are replaced by equal amounts of proline and aspartic acid in the formulation of the medium.
TABLE 2 culture process conditions
Test examples
The strains after culturing in examples 1 to 7 and comparative examples 1 to 6 were tested, and the results are shown in Table 3, wherein the unit of the unit bacterial load of the bacterial powder is hundred million cfu/g, the unit of the unit bacterial load of the bacterial liquid is hundred million cfu/mL, the bacterial powder is the composite bacterial load for sewage treatment before culturing, the bacterial liquid is the bacterial-containing liquid obtained after culturing, and the total bacterial load after expansion/before expansion = the unit bacterial load (total) of the bacterial liquid after culturing x the volume/the amount of the composite bacterial load for sewage treatment before culturing x the unit bacterial load of the bacterial load. Taking example 1 as an example, total bacterial load after expansion/before expansion=10.6× (300×65% +3000×60%)/(300×65% ×2.5% ×30) =145 (rounded).
Table 3 test results
TABLE 3 (follow-up) test results
From this, it can be seen that the culture scheme (including the composition of the medium and the culture process conditions) provided in example 1 can obtain an optimal expansion effect. The comparative example 1 shows that the total bacterial load is worse than that of the example 1 after the expansion culture, and the ratio of aerobic bacteria to anaerobic bacteria is obviously unbalanced, so that a certain synergistic effect exists among sodium thiosulfate, sodium bicarbonate and dipotassium hydrogen phosphate used in the application, and the effect of the sodium thiosulfate is irreplaceable. Comparative example 2 shows a lower propagation effect than example 1, indicating that there is some synergy between the yeast extract, peptone and urea used in this application, and urea is not an alternative. Comparative example 3 shows that the effect of the culture medium is worse than that of example 1, and that the cysteine and methionine have direct influence on the effect of the culture medium, and that the effect of the culture medium is obviously reduced by replacing the cysteine and methionine with other amino acids. Comparative example 4 the total bacterial load after the expansion was close to that of example 1, but the ratio of aerobic and anaerobic bacteria was severely unbalanced, demonstrating that this application. Comparative example 5 has a worse expansion effect than example 1, indicating that the process of 6-18h in this application has a direct effect on expansion effect. The total bacteria difference of comparative example 6 is not large compared with that of comparative example 1 after the expansion culture, but the ratio of aerobic bacteria to anaerobic bacteria is obviously unbalanced, which shows that the process of 18-24 hours in the application has direct influence on the balance of the aerobic bacteria and the anaerobic bacteria in the bacterial liquid.
Further, the strain cultured in the above example 1 was subjected to a sewage treatment test, specifically, sewage in the same sewage tank was inoculated with the bacterial liquid in the example 1 and the original bacterial powder in different proportions, and then naturally left to stand for sewage treatment, and the specific bacterial liquid/bacterial powder addition ratio is shown in table 4, and the treatment results are shown in table 5.
TABLE 4 addition ratio
| Group 1 | Group 2 | Group 3 | |
| volume/L of sewage | 100 | 100 | 100 |
| Raw bacterial powder amount/kg | - | - | 2 |
| EXAMPLE 1 bacterial liquid amount/L | 6 | 20 | - |
TABLE 5 treatment results
The results of table 3 and table 5 can be combined to give: the unit viable count of the bacterial liquid after the expansion culture is 30.96-35.33% of that of the original bacterial powder, the weight can reach 409.2 times (300 multiplied by 65% +3000 multiplied by 60%)/(300 multiplied by 65% ×2.5%) of the original bacterial powder, namely the total viable count is 127-145 times of that of the inoculated bacterial powder, and the ratio between aerobic bacteria and anaerobic bacteria is not greatly changed compared with that of the bacterial powder, so that the ratio of each bacterial in the bacterial liquid after the expansion culture is not greatly changed, and meanwhile, a part of nutrient still remains for the use of the bacterial liquid for sewage treatment, compared with the use of the composite bacterial powder directly for sewage treatment by using the bacterial liquid after the expansion culture with the weight of 10 times of that of the bacterial powder, the effect is close and the effective time is faster, and the unit bacterial liquid after the expansion culture can reach 400 times of that of the original bacterial powder, namely, when the bacterial liquid with the weight of 10 times of that of the bacterial powder is used for sewage treatment, the bacterial powder can be greatly reduced by 40 times, and the use of the bacterial powder and the sewage treatment cost can be greatly reduced.
In summary, the culture medium provided by the application is prepared by compounding an organic carbon source, an inorganic carbon source, an organic nitrogen source, an inorganic nitrogen source, a metal ion source and a potential regulator according to a specific proportion, so that the obtained culture medium not only can provide nutrients required by strains, but also can maintain good potential and osmotic pressure conditions, thereby being beneficial to the growth and propagation of partial heterotrophic bacteria, improving the competitiveness of anaerobic bacteria and solving the problems of unbalanced growth and even complete inhibition of partial bacterial growth caused by different nutritional requirements of various strains. And by combining a specific culture process, different strains such as aerobic bacteria, anaerobic bacteria, autotrophic bacteria, heterotrophic bacteria and the like can be grown together under the condition of keeping a relative balance, so that the effective viable count is amplified as much as possible under the condition of keeping the relative balance of the number of the composite strains, and further, when sewage treatment is carried out, a good sewage treatment effect can be obtained under the condition of greatly reducing the using amount of bacterial powder, and the sewage treatment cost is reduced.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The culture method of the strain for sewage treatment is characterized by comprising the following steps: inoculating a to-be-cultured substance containing a strain for sewage treatment into a first container containing a culture medium for first culture; transferring the material after the first culture into a second container containing the culture medium for expansion culture;
the culture conditions during the first culture include:
in the process of culturing for 0-6h, the stirring rotation speed is 45-55r/min, the ventilation ratio is 0.78-0.86, and the temperature is 22-24 ℃;
in the process of culturing for 6-12h, the stirring rotation speed is 140-160r/min, the ventilation ratio is 1.20-1.30, and the temperature is 24-26 ℃;
in the process of culturing for 12-18h, the stirring rotation speed is 200-220r/min, the ventilation ratio is 1.62-1.74, and the temperature is 30-32 ℃;
in the process of culturing for 18-24h, the stirring rotation speed is 80-90r/min, the ventilation ratio is 0.36-0.42, and the temperature is 36-38 ℃;
the culture conditions of the expansion culture are the same as those in the first culture process;
each 100 parts of the culture medium comprises 0.6-0.8 part of organic carbon source, 0.44-0.6 part of organic nitrogen source, 0.8-1 part of inorganic carbon source, 0.11-0.116 part of inorganic nitrogen source, 0.1246-0.249 part of metal ion source, 0.106-0.17 part of potential regulator and the balance of water according to parts by weight;
the organic carbon source is glucose, and the inorganic carbon source comprises at least one of sodium bicarbonate and potassium bicarbonate;
the organic nitrogen source comprises, by weight, 0.16-0.2 part of yeast extract, 0.16-0.2 part of peptone, 0.1-0.16 part of urea, 0.01-0.02 part of cysteine and 0.01-0.02 part of methionine;
the inorganic nitrogen source comprises 0.1-0.16 part of ammonium sulfate and 0.01-0.016 part of sodium nitrate;
the metal ion source comprises 0.1-0.2 part of magnesium sulfate, 0.0006-0.001 part of manganese sulfate, 0.02-0.04 part of calcium chloride, 0.001-0.002 part of zinc chloride, 0.001-0.002 part of cobalt chloride, 0.001-0.002 part of ferrous sulfate and 0.001-0.002 part of ammonium molybdate;
the potential regulator comprises 0.1-0.16 part of sodium thiosulfate and 0.006-0.01 part of dipotassium hydrogen phosphate;
the culture medium is used for culturing a strain for sewage treatment; the strain for sewage treatment is a glycerol composite strain GANDEW-MIX.
2. The method according to claim 1, wherein the amount of the culture to be inoculated during the first cultivation is 2 to 3% by weight of the medium.
3. The culture method according to claim 1, wherein the alkaline solution is supplied periodically during the first culture so that the pH in the first vessel is not lower than 6.8 and not higher than 8.0.
4. The method according to claim 3, wherein the lye is an aqueous sodium hydroxide solution having a concentration of 8 to 12 wt%.
5. The culture method according to claim 1, wherein the volume of the second container is 10 to 20 times the volume of the first container.
6. The method according to claim 1, wherein the transfer is performed by using a sterilized transfer line.
7. Use of the culture method according to any one of claims 1 to 6, wherein the culture method is for culturing a strain for sewage treatment;
the strain for sewage treatment is a glycerol composite strain GANDEW-MIX.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110610762.3A CN113151141B (en) | 2021-06-01 | 2021-06-01 | Culture method of strain for sewage treatment and culture medium thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110610762.3A CN113151141B (en) | 2021-06-01 | 2021-06-01 | Culture method of strain for sewage treatment and culture medium thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113151141A CN113151141A (en) | 2021-07-23 |
| CN113151141B true CN113151141B (en) | 2023-06-16 |
Family
ID=76875461
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110610762.3A Active CN113151141B (en) | 2021-06-01 | 2021-06-01 | Culture method of strain for sewage treatment and culture medium thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113151141B (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468608A (en) * | 2013-08-27 | 2013-12-25 | 重庆瑞宝环保工程有限公司 | Complex flora for sewage treatment and culture method of complex flora |
| CN105462880A (en) * | 2015-12-10 | 2016-04-06 | 南平正大欧瑞信生物科技开发有限公司 | Preparation method of sewage processing complex bacterium |
| CN106085864A (en) * | 2016-06-15 | 2016-11-09 | 天津农学院 | A kind of Aquatic product feeding micro encapsulation compound micro-ecological preparation and preparation method |
| CN109182177A (en) * | 2018-09-11 | 2019-01-11 | 福建傲农生物科技集团股份有限公司 | A kind of compound probiotic deodorant and the preparation method and application thereof |
| CN117568145A (en) * | 2020-06-08 | 2024-02-20 | 云南龙帜环境工程有限公司 | Strain culture device |
-
2021
- 2021-06-01 CN CN202110610762.3A patent/CN113151141B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113151141A (en) | 2021-07-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101899397B (en) | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application | |
| CN106434469B (en) | Low-temperature-resistant nitrifying bacteria agent and preparation method and application thereof | |
| CN111252902A (en) | Process method for treating dehydroacetic acid mixed wastewater and application | |
| CN101921709B (en) | Composite high-efficiency microorganism preparation for synergy of sewage treatment plant and synergistic process | |
| CN112795560A (en) | Biological agent for treating industrial wastewater and preparation method thereof | |
| CN111040970A (en) | Compound microbial agent, preparation method thereof and application thereof in black and odorous water body remediation | |
| CN110143732B (en) | Method for treating waste water generated in vegetable oil byproduct processing | |
| CN113151141B (en) | Culture method of strain for sewage treatment and culture medium thereof | |
| CN109468251B (en) | Thiourea degrading strain and method for treating thiourea-containing wastewater by using same | |
| CN107325982B (en) | Process for advanced treatment of wastewater from production of hydroxymethylcellulose by using microbial preparation | |
| CN109679865A (en) | A kind of microorganism blender and its preparation preparation and application | |
| CN106277286B (en) | Method for eliminating viscous swelling of activated sludge in biochemical system | |
| CN115058357A (en) | Bacterial strain for biological drying of kitchen waste, screening method and application | |
| CN114990032A (en) | Preparation method of microbial fermentation scale inhibitor and prepared microbial fermentation scale inhibitor | |
| CN106554083B (en) | Quick start method for treating ammonia-containing wastewater by A/O process | |
| CN114164153A (en) | Acid-producing bacteria and system and method for one-pot boiling process for treating edible gelatin production wastewater by using same | |
| CN114591859A (en) | Microbial agent formula for water quality improvement and preparation method thereof | |
| CN111099722B (en) | Composition for promoting denitrification and application thereof | |
| CN106497846A (en) | A kind of wastewater treatment microbial microbial inoculum and preparation method thereof | |
| CN106630190B (en) | Fungus agent for degrading COD and preparation method thereof | |
| CN111186917A (en) | Method for treating residues of brewing wastewater by using microbial agent | |
| CN114057291A (en) | Total nitrogen removal growth-promoting medicament and preparation and application thereof | |
| CN104611246A (en) | Synchronous cultivation method of nitrobacteria and aerobic denitrobacteria | |
| CN108977360A (en) | The method that town sewage plant tail water is used for microbial bacterial agent culture | |
| CN109880785B (en) | Composition capable of promoting denitrification of nitrifying bacteria and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20230529 Address after: 343700 Taihe County Industrial Park, Ji'an City, Jiangxi Province Applicant after: Ji'an aonong Modern Agricultural Technology Co.,Ltd. Applicant after: TAIHE AOMU BREEDING CO.,LTD. Applicant after: FUJIAN AONONG BIOLOGICAL TECHNOLOGY GROUP Co.,Ltd. Address before: 363000 intersection of Xingting Road and Baolian Road, Jinfeng Economic Development Zone, Xiangcheng District, Zhangzhou City, Fujian Province Applicant before: FUJIAN AONONG BIOLOGICAL TECHNOLOGY GROUP Co.,Ltd. Applicant before: TAIHE AOMU BREEDING CO.,LTD. Applicant before: Ji'an aonong Modern Agricultural Technology Co.,Ltd. Applicant before: SHANGHANG AONONG HUAI PIG INDUSTRY DEVELOPMENT CO.,LTD. Applicant before: XIAMEN JIAYEXING AGRICULTURAL TECHNOLOGY Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230820 Address after: 343799 Taihe County Industrial Park, Ji'an City, Jiangxi Province Patentee after: Ji'an aonong Modern Agricultural Technology Co.,Ltd. Patentee after: TAIHE AOMU BREEDING CO.,LTD. Patentee after: Fujian Aoxin Biotechnology Group Co.,Ltd. Patentee after: FUJIAN AONONG BIOLOGICAL TECHNOLOGY GROUP Co.,Ltd. Address before: 343700 Taihe County Industrial Park, Ji'an City, Jiangxi Province Patentee before: Ji'an aonong Modern Agricultural Technology Co.,Ltd. Patentee before: TAIHE AOMU BREEDING CO.,LTD. Patentee before: FUJIAN AONONG BIOLOGICAL TECHNOLOGY GROUP Co.,Ltd. |
|
| TR01 | Transfer of patent right |