CN113142207A

CN113142207A - Stranguria agnus disorienting agent and application thereof

Info

Publication number: CN113142207A
Application number: CN202110497382.3A
Authority: CN
Inventors: 梅向东; 任梓齐; 赵猛; 刘兴强; 赵崇强; 褚兆辉; 王留洋; 李慧; 马好运; 宁君
Original assignee: Tengzhou Plant Protection And Inspection Station; Northeast Forestry University; Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Current assignee: Tengzhou Plant Protection And Inspection Station; Northeast Forestry University; Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Priority date: 2021-05-07
Filing date: 2021-05-07
Publication date: 2021-07-23
Anticipated expiration: 2041-05-07
Also published as: CN113142207B

Abstract

The invention discloses a strange noctuid disorienting agent and application thereof. The strange noctuid disorienting agent is cis-11-hexadecylene-benzoate, and the cis-11-hexadecylene-benzoate is added into a PVC capillary to prepare the strange noctuid disorienting agent luring core which has better disorienting effect on strange noctuids in fields. The luring core of the noctuid disorienting agent contains cis-11-hexadecenyl-benzoate with the mass of 0.8-1.8 mg. The disorientation agent control technology is environment-friendly, safe in natural enemies, strong in specificity, easy to obtain raw materials and simple and convenient to operate, can play a role in interfering and disorienting, and has important significance in comprehensively treating the strange noctuids.

Description

Stranguria agnus disorienting agent and application thereof

Technical Field

The invention relates to the technical field of insect sex pheromone preparation, and particularly relates to a noctuid disorienting agent and application thereof.

Background

The stranguria (Trachea atriplicis Linnaeus) also called Knoxia leucoptera, belongs to Lepidoptera and Noctuidae, and is one of important agricultural pests. The plant leaves such as polygonum, rumex acetosa, humifuse euphorbia herb, orychophragmus violaceus and the like are eaten, the whole plant growth process is damaged, and serious economic loss of crops can be caused. The distribution of the strange noctuids is worldwide, such as europe, japan, russia; the fertilizer is mainly distributed in Beijing, Hebei, Henan, Shanghai, Jiangsu, Heilongjiang and other places in China. Adults were visible under the light of Beijing 6-9 months. For a long time, the method for preventing and controlling the strange noctuids has the advantages that firstly, because the imagoes have phototaxis, the imagoes can be trapped and killed by using the black light lamp in the high-light-emitting period, although the method has the effect, the application of the black light lamp has certain limitation due to the reasons of electric power, economic conditions and the like; secondly, chemical agents are adopted, but chemical pesticides are easy to generate drug resistance and kill natural enemies, and simultaneously, the problems of pesticide residue, environmental pollution and the like can also occur. Thirdly, an insect sex pheromone trapping method is adopted, although the method is environment-friendly, the trapping amount of pests is limited, and for various pests, a plurality of sets of trapping devices are required, so that the cost is high.

Disclosure of Invention

In view of the above, the present invention aims to provide a novel environmental-friendly and efficient trichoplusia ni disorientation agent and an application thereof, so as to achieve a novel biological control effect.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention discloses a strange noctuid disorienting agent which is cis-11-hexadecenyl-benzoate. The amount of the stranguria agnea disorientation agent is 140-650mg/hm²。

An application of the strange noctuid disorientation agent can be used for preparing a strange noctuid disorientation lure, and the preparation method comprises the following steps: adding a chlorinated paraffin solution containing cis-11-hexadecenyl-benzoate serving as the noctuid disorienting agent into a PVC capillary to prepare the luring core of the noctuid disorienting agent.

Optionally, the mass of cis-11-hexadecenyl-benzoate contained in the luring core of the noctuid disorienting agent is 0.8-1.8 mg.

Preferably, the luring core of the noctuid disorienting agent contains cis-11-hexadecenyl-benzoate with the mass of 1-1.5 mg.

Optimally, the luring core of the noctuid disorienting agent contains cis-11-hexadecenyl-benzoate with the mass of 1.5 mg.

The luring core of the disorientation agent is placed in a trap, the trap is placed in a region where the armyworm occurs or is likely to occur, and the placement number of the trap is 180-²。

The strange noctuid disorienting agent can effectively interfere a communication system between male and female insects of strange noctuids, so that the antennae of the male insects are in a paralyzed state after contacting with high-concentration sex pheromones for a long time, and the sexual calling reaction capability to the female insects is lost, thereby reducing the mating rate of the male and female insects and furthest reducing the harm of next generation of larvae.

Compared with the prior art, the invention has the following beneficial effects: the disorientation agent disclosed by the invention can be used for preventing and treating strange noctuids, can reduce the use amount of chemical pesticides, protect the environment, has an outstanding prevention effect and strong specificity, is beneficial to protecting natural enemies, and can reduce the mating rate of the strange noctuids by 90%; meanwhile, the release device is slightly influenced by natural conditions, the raw materials of the release device are easy to obtain, the operation is simple, the stability is good, the lasting period is long, the lasting period of the PVC capillary release device can reach 100-plus-150 days, the popularization is easy, and great social benefits and ecological benefits are achieved.

Drawings

FIG. 1 is a schematic diagram of the chemical structure of cis-11-hexadecenyl-benzoate;

FIG. 2 is a schematic diagram of the synthetic route of cis-11-hexadecenyl-benzoate.

Detailed Description

The present invention will be described in detail below with reference to the drawings and examples, but the practice of the invention is not limited thereto.

EXAMPLE 1 preparation of cis-11-hexadecenyl-benzoate

The chemical structural formula of the compound cis-11-hexadecenyl-benzoate in the invention is shown in figure 1, and the synthetic route is shown in figure 2.

1.0g (4.16mmol) of cis-11-hexadecenol was dissolved in 25mL of a solution of N, N-Dimethylformamide (DMF), and 0.86g (6.24mmol) of K was added₂CO₃And (3) as an acid-binding agent, stirring under an ice salt bath condition, slowly dropwise adding a DMF (dimethyl formamide) solution containing 0.88g (6.24mmol) of benzoyl chloride when the temperature is reduced to about-20 ℃, reacting the system for about 30min under the condition, slowly heating to room temperature after removing the ice salt bath, continuing to react overnight, monitoring by TLC (thin layer chromatography) in the reaction process, and adding a small amount of deionized water to quench the reaction when the reaction raw materials are not reduced any more. The organic layer of the reaction solution was extracted with ethyl acetate (10ml x3 times), the combined extracts were washed with a saturated saline solution (25ml x3 times), dried over anhydrous magnesium sulfate for 40min, filtered and rotary-distilled under reduced pressure. Silica gel column chromatography (ethyl acetate: petroleum ether ═ 1: 10) afforded the title compound. The product was a yellow oil in 79% yield.

Example 2 field bioactivity assay

Selecting varieties,And (4) taking the plots with basically the same seeding time, growth vigor and daily management measures as test plots. Will be located at 0.2hm of the upper tuyere²The area of the land is used as a control area, 3 traps containing stranguria looper sex pheromone attraction cores are respectively and uniformly placed in the center of the land in a regular triangle way to be used as a blank control, and the height of each trap is about 1 m; lower tuyere 0.2hm²The area is used as a disorientation area, 180 barrel-shaped traps containing the strange noctuid disorientation agent luring cores are evenly placed in each hectare, and 3 traps containing the strange noctuid sex pheromone luring cores are respectively and evenly placed in the center of the disorientation area by a regular triangle for monitoring; 0.2hm in the middle²The area of (a) serves as an isolation area without any traps being placed.

Wherein 3 replicates of each disorienting agent formula are set in the disorienting region, the disorienting rates are averaged (analyzed with SPSS data processing software), and the specific dosage, placement number and disorienting rate of the disorienting agent are shown in table 1.

(wherein the expression of disorientation rate: disorientation rate (%) (1-amount of moth-trapping in disorientation zone/amount of moth-trapping in control zone)). times.100%)

TABLE 1

Comparing the formulas 1, 2, 3, 4 and 5, it can be seen that the disorientation rate tends to increase with the increase of the dosage of the strange noctuid disorientation agent; as can be seen by comparing formulations 4, 6, 7, 8, and 9, the disorientation rate gradually increases as the number of traps increases; as can be seen by comparing formulations 4, 5 and 9, there was a tendency for disorientation rate to increase with increasing dose of disorientation agent and number of traps, but in P>At 0.05, there was no significant difference, and from the aspect of agricultural use cost, the dosage of the disorienting agent added to the individual traps was 1.5mg, and the number of traps set was 315 per hm²The disorientation effect is the best, the disorientation rate can reach more than 91%, and simultaneously under the guidance of the scheme, the purposes of the highest economic benefit and the best prevention and treatment effect are achieved. The cis-11-hexadecenyl-benzoate is used as a disorientation agent of the armywormThe green high-efficiency defense measurement provides technical support.

The above disclosure is only for the specific embodiment of the present invention, but the present invention is not limited thereto, and any variations that can be made by those skilled in the art should fall within the scope of the present invention.

Claims

1. The strange noctuid disorienting agent is characterized by being cis-11-hexadecenyl-benzoate.

2. The use of the noctuid disorienting agent as claimed in claim 1, wherein the amount of the disorienting agent is 140-650mg/hm²。

3. The use of the noctuid disorienting agent of claim 1, wherein the noctuid disorienting agent is cis-11-hexadecenyl-benzoate added to PVC capillaries to produce a lure of the noctuid disorienting agent, which is placed in traps, which are placed in areas where the noctuids occur or are likely to occur.

4. The application of the noctuid disorienter according to claim 3, wherein the lure core of the noctuid disorienter contains cis-11-hexadecenyl-benzoate in an amount of 0.8-1.8 mg.

5. The application of the strange noctuid disorienter of claim 3, wherein the lure core of the strange noctuid disorienter contains cis-11-hexadecenyl-benzoate in an amount of 1-1.5 mg.

6. The use of the noctuid disorienting agent as claimed in claim 3, wherein the number of traps is 180-²。