CN113141989A - Method for increasing content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits - Google Patents
Method for increasing content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits Download PDFInfo
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- 241000219112 Cucumis Species 0.000 title claims abstract description 68
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 63
- FZAQROFXYZPAKI-UHFFFAOYSA-N anthracene-2-sulfonyl chloride Chemical compound C1=CC=CC2=CC3=CC(S(=O)(=O)Cl)=CC=C3C=C21 FZAQROFXYZPAKI-UHFFFAOYSA-N 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 49
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims abstract description 46
- 229960003512 nicotinic acid Drugs 0.000 claims abstract description 40
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims abstract description 35
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 35
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- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 15
- 229950006238 nadide Drugs 0.000 description 12
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- JLEBZPBDRKPWTD-TURQNECASA-O N-ribosylnicotinamide Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](CO)O2)O)=C1 JLEBZPBDRKPWTD-TURQNECASA-O 0.000 description 8
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 7
- 235000001968 nicotinic acid Nutrition 0.000 description 6
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- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 241000219122 Cucurbita Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
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- DAYLJWODMCOQEW-TURQNECASA-N NMN zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)([O-])=O)O2)O)=C1 DAYLJWODMCOQEW-TURQNECASA-N 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
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- 230000005078 fruit development Effects 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
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- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 229940101270 nicotinamide adenine dinucleotide (nad) Drugs 0.000 description 1
- 235000020956 nicotinamide riboside Nutrition 0.000 description 1
- 239000011618 nicotinamide riboside Substances 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
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- 238000009777 vacuum freeze-drying Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 150000003698 vitamin B derivatives Chemical class 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for improving the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits, which comprises the following steps: adding water into a container, adding vitamin B3, stirring to dissolve and mix uniformly, adding active protein, stirring thoroughly, mixing uniformly, standing to obtain a mixed solution, and irrigating and spraying onto leaf surface when the development of Cucurbitaceae melon and fruit is required. Preferably, 1000kg of water, 1kg of vitamin B3, 50g of active protein are added to the process. The obtained mixed solution is irrigated once in seedling stage, fruiting stage and mature stage of Cucurbitaceae melon and fruit. The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits provided by the invention can be used for manually controlling the conversion of VB3 and derivatives thereof in the cucurbitaceae melons and fruits into NMN, so that the NMN in the cucurbitaceae melons and fruits is effectively and remarkably improved, and the cucurbitaceae melons and fruits rich in NMN are supplied to the market, so that consumers are healthier.
Description
Technical Field
The invention relates to a method for effectively improving the content of NMN in cucurbitaceae melons and fruits in the fields of biotechnology and plant physiology, in particular to a method capable of effectively improving the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits.
Background
Beta-nicotinamide mononucleotide, nmn (nicotinamide mononucleotide), is a naturally occurring biologically active nucleotide. NMN has 2 forms of irregularity, α and β; the beta isomer is the active form of NMN. Since niacinamide belongs to vitamin B3, NMN belongs to the category of vitamin B derivatives, and is widely involved in various biochemical reactions of human body, closely related to immunity and metabolism.
NMN is an intrinsic substance in the human body and is also abundant in some fruits and vegetables. In humans NMN is the most direct precursor of NAD +, whose function is represented by NAD +. NAD + is also called coenzyme I, and is known as nicotinamide adenine dinucleotide, and is involved in thousands of reactions in every cell. In various cellular metabolic reactions, Nicotinamide Adenine Dinucleotide (NAD) molecules play an important role and are an important support for cell viability. The decline of NAD during aging is considered to be a major cause of diseases and disabilities such as hearing and vision loss, cognitive and motor dysfunction, immune deficiency, arthritis due to autoimmune inflammatory response disorders, metabolic disorders and cardiovascular diseases. Therefore, NMN supplementation increases NAD + levels in vivo, thereby delaying, ameliorating, and preventing aging-related phenotypes, or age-induced metabolic disorders, geriatric diseases, and the like.
The cucurbitaceae is a plant in the plant field, more than 800 plants exist, and more than 130 plants exist in China, wherein the cucurbitaceae comprises common vegetables and melons and fruits such as cucumbers, pumpkins, loofah, watermelons and the like. Cucurbitaceae melons and fruits are frequently eaten in daily life, but the content of NMN in cucurbitaceae melons and fruits is low. As the relevant data is searched and inquired, the similar products of cucurbitaceae melons and fruits rich in NMN or the method for producing and manufacturing the products thereof are not found at home and abroad. At present, only the United states adopts a transgenic technology, so that the content of crop green soy beans with the highest NMN content in the nature is further increased, and then NMN is purified to prepare a medicine.
Therefore, if a method for effectively improving the content of NMN (beta-nicotinamide mononucleotide) in cucurbitaceae melons and fruits is developed, the cucurbitaceae melons and fruits rich in NMN can be supplied to the market, the health of consumers is benefited, and the method can be popularized and sold all over the world and has wide prospects.
Disclosure of Invention
The invention aims to provide a method for effectively improving the content of NMN in cucurbitaceae melons and fruits, belongs to the field of biotechnology or agricultural science and technology, can solve the existing problems, and can artificially control the conversion of VB3 and derivatives thereof in the cucurbitaceae melons and fruits into the NMN.
In order to achieve the aim, the invention provides a method for improving the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits, wherein the method comprises the following steps: adding water into a container, adding vitamin B3, stirring to dissolve and mix uniformly, adding active protein, stirring thoroughly, mixing uniformly, standing to obtain a mixed solution, and irrigating and spraying onto leaf surface when the development of Cucurbitaceae melon and fruit is required.
The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits comprises the steps of adding two thirds of water into a water tank, adding food-grade vitamin B3, stirring, dissolving, uniformly mixing, adding the active protein, and uniformly stirring for use.
The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits comprises the step of mixing vitamin B3 and water according to the mass ratio of 1 (800-1200).
The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits is characterized in that in the method, the mass ratio of active protein to water is (4-8): (1X 10)5)。
The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits comprises the steps of adding 1000kg of water, 1kg of vitamin B3 and 50g of active protein.
The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits is characterized in that the active protein is prepared by microbial fermentation and extraction.
The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits comprises the following steps of adding active protein, fully stirring, uniformly mixing, standing for 10 minutes, and using the obtained mixed solution.
The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits is characterized in that the mixed solution is prepared and used at the temperature of 15-30 ℃.
The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits comprises the step of respectively irrigating the mixed solution once at the seedling stage, the fruiting stage and the early stage of maturation of the cucurbitaceae melons and fruits.
The method for improving the content of the beta-nicotinamide mononucleotide in the cucurbitaceae melons and fruits is characterized in that the water content of the soil is adjusted to 30% before the mixed solution is irrigated.
The method for improving the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits provided by the invention has the following advantages:
NMN is a derivative of vitamin B3, originally exists in natural animals and plants, is a precursor of coenzyme I, namely NAD + (beta-nicotinamide adenine dinucleotide), in human bodies, has the effects of participating in DNA repair, regulating cellular oxidation pressure and resisting aging, but has low content in cucurbits and melons. The invention adopts a biological enzyme auxiliary technology to convert VB3 (vitamin B3) and derivatives thereof in the cucurbitaceae melons to NR (nicotinamide ribose) and NMN, and then convert NR to NMN, so that the NMN in the cucurbitaceae melons is effectively and obviously improved, the cucurbitaceae melons rich in NMN can be supplied to the market, and consumers are more healthy.
Detailed Description
The following further describes embodiments of the present invention.
The invention provides a method for improving the content of beta-Nicotinamide Mononucleotide (NMN) in cucurbitaceae melons and fruits, which comprises the following steps: adding water into a container, adding vitamin B3, namely nicotinic acid, according to a certain proportion, stirring to fully dissolve and uniformly mix the nicotinic acid, adding active protein, fully stirring, uniformly mixing, standing to obtain a mixed solution, and finally irrigating and spraying leaf surfaces at a proper period required by the development of cucurbitaceae melons and fruits.
Preferably, two thirds of water is added into a water tank with a certain volume, then food-grade vitamin B3 is added, stirred to dissolve and uniformly mix, and then active protein is added.
The mass ratio of the vitamin B3 to the water is 1 (800-1200), and the better ratio is 1: 1000.
The mass ratio of the active protein to the water is (4-8): (1X 10)5) More preferably 5: (1X 10)5)。
Most preferably, 1000kg of water, 1kg of vitamin B3, 50g of active protein are added.
The active protein is an active substance prepared by microbial fermentation, preferably an enzyme.
Active protein refers to a class of proteins that have some specific physiological functions in addition to the nutritional effects of general proteins. The active protein is a kind of protein with biological activity, like protein, it is a kind of long-chain high molecular compound formed by combining several amino acids, and it has the general properties of all proteins. The requirement of active protein on environment is high, high-concentration biological protein is easy to lose activity when being stored at normal temperature, in order to ensure the special activity and function of the rare components, a biological freeze-drying process is needed, and an ultra-low-speed ultra-low-temperature vacuum freeze-drying technology at minus 80 ℃ is adopted in an aseptic environment, so that the biological active protein enters a dormant state, and the aim of completely storing the biological activity is fulfilled.
In the method, active protein is added, the mixture is fully stirred and is kept stand for 10 minutes after being uniformly mixed, and the obtained mixed solution can be used.
In the method, the mixed solution is prepared and used at the temperature of 15-30 ℃.
The obtained mixed solution is irrigated once in seedling stage, fruiting stage and mature stage of Cucurbitaceae melon and fruit. The mixed liquid should be directly irrigated to the roots of the plants and sprayed on the leaves to promote absorption.
The water content of the soil is adjusted to 30 percent before the mixed liquid is irrigated. That is, the mixed solution is poured into soil having a water content of about 30%.
The method of the invention sprays VB3 biological enzyme auxiliary agent to plants in different development periods of cucurbitaceae melons and fruits to promote the conversion of VB3 (vitamin B3) in the cucurbitaceae melons and fruits to NR (nicotinamide riboside) and NMN (beta-nicotinamide mononucleotide), so that the NMN content in the cucurbitaceae melons and fruits is obviously improved, as shown in the following.
VB3+ enzyme NR + enzyme NMN
VB3+ enzyme-NMN
The method for increasing the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits provided by the invention is further described with reference to the following examples.
Examples 1 to 4
The embodiments respectively provide a method for improving the content of beta-Nicotinamide Mononucleotide (NMN) in cucurbitaceae melons and fruits, which comprises the following steps:
adding two thirds of water into a water tank with a certain volume, then adding food-grade vitamin B3, namely nicotinic acid, into the water tank according to a certain proportion, fully dissolving the nicotinic acid by stirring, uniformly mixing the nicotinic acid, adding active protein, fully stirring, uniformly mixing, standing for 10 minutes to obtain a mixed solution, and finally irrigating and spraying the leaves of the cucurbitaceae melons and fruits at a proper period required by the development of the cucurbitaceae melons and fruits.
In the method, the mixed solution is prepared and used at the temperature of 15-30 ℃. The obtained mixed solution is irrigated once in seedling stage, fruiting stage and mature stage of Cucurbitaceae melon and fruit. The mixed solution should be directly irrigated to the roots of the plants to promote absorption. The water content of the soil is adjusted to 30 percent before the mixed liquid is irrigated.
The raw material composition distribution ratios of the examples are shown in table 1 below. The quality of each raw material component reaches the corresponding national standard.
Table 1: raw material table of mixed solution (by weight).
| Example 1 | Example 2 | Example 3 | Example 4 | |
| Water (W) | 1000kg | 800kg | 1200kg | 1100kg |
| Vitamin B3 | 1.1kg | 1kg | 1kg | 1kg |
| Active protein | 50g | 40g | 80g | 60g |
Example 5
Under the condition that the temperature is 20-25 ℃, two thirds of water is added into a constant volume water tank, a certain proportion of food-grade VB3 is fully dissolved, active protein extracted by microbial fermentation is added, the mixture is fully stirred and kept stand, roots are irrigated at a proper period of cucurbitaceae melon and fruit development, and absorption is promoted.
Preferably, 1000kg of water is used, 1kg of vitamin B3 is added, the mixture is fully stirred, 50g of active protein is added, the mixture is fully stirred and stands for ten minutes, and then the active protein can be used. The fruit is irrigated once in the fruiting period of cucurbitaceae melons and fruits and irrigated once in the early stage of maturation.
In the implementation process of the method, the specific content of the NMN in each batch of cucurbitaceae melons and fruits is different under the influence of factors such as temperature, humidity, illumination, wind speed and the like.
Preferred conditions of use are: the temperature is 15-30 ℃; the soil moisture content is about 30 percent; the weather conditions are in the morning when the weather is not rainy (sunny or cloudy), and in the condition of low wind power.
The method of this example was pilot tested and demonstrated in the hami melon base of southern hai of white shacun, city, autonomous, le, to the southeast, le, li, province.
The reaction process is as follows.
VB3 (vitamin B3), also called niacin, with the molecular formula C6H5NO2
Enzyme ↓
NR (nicotinamide ribose), formula C11H16N2O5
Enzyme ↓
NMN (beta-nicotinamide mononucleotide), molecular formula C11H15N2O8P
Enzyme ↓
NAD + (beta-nicotinamide adenine dinucleotide), molecular formula is C21H27N7O14P2
Through tests and detection, the first specific data of VB3 and derivatives thereof absorbed by cucurbitaceae melons and fruits in different development stages are mastered. The test results are shown in table 2 below.
Table 2: the result of the detection
The method for improving the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits provided by the invention can artificially control the conversion of VB3 and derivatives thereof in cucurbitaceae melons and fruits to NMN, and other similar products and methods are not found so far. The method of the invention adopts the bio-enzyme auxiliary technology to convert VB3 (vitamin B3) and derivatives thereof in cucurbitaceae melons and fruits into NMN, so that the content of the VB3 and the derivatives thereof is improved, and the melons and fruits rich in NMN are supplied to the market. Cucurbitaceae melons and fruits are frequently eaten in daily life, and after people eat melons and fruits rich in NMN, the melons and fruits are converted into NAD + coenzyme 1 (nicotinamide adenine dinucleotide) in human bodies, so that the medical science proves that the melons and fruits are very beneficial to health.
While the present invention has been described in detail with reference to the preferred embodiments, it should be understood that the above description should not be taken as limiting the invention. Various modifications and alterations to this invention will become apparent to those skilled in the art upon reading the foregoing description. Accordingly, the scope of the invention should be determined from the following claims.
Claims (10)
1. A method for increasing the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits is characterized by comprising the following steps: adding water into a container, adding vitamin B3, stirring to dissolve and mix uniformly, adding active protein, stirring thoroughly, mixing uniformly, standing to obtain a mixed solution, and irrigating and spraying onto leaf surface when the development of Cucurbitaceae melon and fruit is required.
2. The method of claim 1, wherein the method comprises adding two thirds of water into a water tank, adding food-grade vitamin B3, stirring to dissolve, mixing uniformly, and adding active protein.
3. The method for increasing the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits according to claim 2, wherein the mass ratio of vitamin B3 to water is 1 (800-1200).
4. The method for increasing the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits as claimed in claim 2, wherein in the method, the mass ratio of active protein to water is (4-8): (1X 10)5)。
5. The method for increasing the content of beta-nicotinamide mononucleotide of cucurbitaceae melons and fruits according to claim 2, wherein 1000kg of water, 1kg of vitamin B3 and 50g of active protein are added.
6. The method for increasing the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits as claimed in claim 5, wherein the active protein is prepared by microbial fermentation and extraction.
7. The method according to claim 1, wherein the active protein is added, the mixture is stirred thoroughly, the mixture is left for 10 minutes after being mixed uniformly, and the obtained mixture is ready for use.
8. The method for increasing the content of beta-nicotinamide mononucleotide in cucurbitaceae melons and fruits as claimed in claim 7, wherein the preparation and use of the mixed solution are performed at the temperature of 15-30 ℃.
9. The method of claim 8, wherein the mixture is irrigated once each at the seedling stage, fruiting stage and early stage of ripening of the cucurbitaceae melon and fruit.
10. The method of claim 9, wherein the mixture is used to adjust the water content of the soil to 30% before irrigation.
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| CN115735686A (en) * | 2022-11-29 | 2023-03-07 | 福大生命科技(广州)有限公司 | Cultivation method for increasing Nicotinamide Mononucleotide (NMN) content in broccoli |
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| CN112646851A (en) * | 2019-10-10 | 2021-04-13 | 安徽古特生物科技有限公司 | Method for rapidly preparing beta-nicotinamide mononucleotide by enzyme method |
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| CN112646851A (en) * | 2019-10-10 | 2021-04-13 | 安徽古特生物科技有限公司 | Method for rapidly preparing beta-nicotinamide mononucleotide by enzyme method |
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| 路福平等: "《微生物学》", vol. 2, 中国轻工业出版社, pages: 291 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115735686A (en) * | 2022-11-29 | 2023-03-07 | 福大生命科技(广州)有限公司 | Cultivation method for increasing Nicotinamide Mononucleotide (NMN) content in broccoli |
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Application publication date: 20210723 |