CN113135542A

CN113135542A - Biological immune cell culture solution extraction system and extraction method thereof

Info

Publication number: CN113135542A
Application number: CN202110486918.1A
Authority: CN
Inventors: 王丹
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-05-03
Filing date: 2021-05-03
Publication date: 2021-07-20

Abstract

The invention relates to the field of extraction of biological immune cell culture solution, in particular to a biological immune cell culture solution extraction system, which comprises: the base, the middle part fixedly connected with culture solution storage box at base top, the right side fixedly connected with booster pump at culture solution storage box top. According to the invention, through the mutually matched use of the two clamping plates and the arc-shaped block, when a filling bottle falls between the two clamping plates and the arc-shaped block, and the rotary circular table rotates, the two clamping plates are opened towards two sides under the action of the guide block, and the two clamping plates can elastically rotate, so that after the filling bottle is completely contacted with the sponge plate, the filling bottle can be wrapped by the two clamping plates and the arc-shaped block under the elastic action of the reset spring, thus the moving stability of the filling bottle can be increased, and the filling bottle can be ensured to be always positioned at a filling point when passing through the guide block every time, the stability of a filling liquid is ensured, and the problem of liquid overflow is avoided.

Description

Biological immune cell culture solution extraction system and extraction method thereof

Technical Field

The invention relates to the field of extraction of biological immune cell culture solution, in particular to a biological immune cell culture solution extraction system and an extraction method thereof.

Background

The extraction of the biological immune cell culture solution mainly comprises the steps of filling the biological immune cell culture solution into a container and sealing the container in the extraction process.

Although the existing biological immune cell culture solution extraction system is also bottled in a flow line production mode and then sealed and conveyed, the equipment can only fill biological immune cell culture solution into one bottled container at a time, when the supply of the container is slow, the continuity is poor, the efficiency is very slow, and each flow is separately carried out, so that the production delay is caused.

The present inventors have been made to solve the above-described problems.

Disclosure of Invention

In order to achieve the purpose, the invention adopts the main technical scheme that: a biological immune cell culture fluid extraction system, comprising: the culture medium storage box is fixedly connected to the middle of the top of the base, a pressurizing air pump is fixedly connected to the right side of the top of the culture medium storage box, a pressure balancing device is fixedly connected to the right side of the culture medium storage box, a pressurizing extraction mechanism is fixedly communicated with the top of the culture medium storage box, a driving motor is fixedly connected to the right side of the top of the base, a driving wheel is fixedly connected to the output end of the driving motor, a one-way air outlet valve is sleeved below the inner cavity of the pressurizing extraction mechanism and at the connection position with the culture medium storage box, a one-way air inlet valve is sleeved on the front side of the inner wall of the pressurizing extraction mechanism, two angle motors are fixedly connected below the side surface of the pressurizing extraction mechanism, a tooth shaft is fixedly connected to the output end of each angle motor, a rotating sleeve is sleeved at the middle of the pressurizing extraction mechanism, a fixed sleeve is sleeved on the rotating sleeve, and an annular tooth socket is arranged below the side surface of the fixed sleeve, the meshing of tooth axle is in the annular tooth's socket, mechanism's inner chamber middle part intercommunication is drawed in the pressure boost has two feed liquor pipes, the bottom of feed liquor pipe is run through the top of culture solution storage box and is extended to the below of inner chamber, the pressure boost is drawed and is constructed and be located the top cover of fixed cover is equipped with reinforced slewing mechanism, reinforced slewing mechanism's bottom fixed connection be in the top fixed connection of fixed cover, base top fixedly connected with fixed stay cover, fixed stay cover top fixedly connected with bottling feeding mechanism, the cover is equipped with recovery unit on the fixed stay cover, the drive wheel pass through conveyer belt with the recovery unit transmission is connected, both sides all are provided with limit baffle around the bottling feeding mechanism, just be located on the base recovery unit below is provided with the collection box.

Preferably, the bottling supply mechanism comprises an annular rail, the annular rail is fixedly connected with an annular blanking mechanism, the top of the annular blanking mechanism is communicated with a plurality of bottle blanking groove stations, the top of the annular rail is positioned at the bottle blanking groove stations and provided with a bottle stopper supply station, the top of the inner wall of the annular rail is provided with a blanking station at a distance from the bottle blanking groove stations and the bottle stopper supply station, two guide blocks are arranged between the bottle blanking groove stations and the bottle stopper supply station, the bottom of the inner cavity of the bottle stopper supply station is provided with an inclined cavity, the inclined cavity is communicated with the blanking cavity, an annular sleeve is sleeved below the inner cavity of the bottle stopper supply station, the inner wall of the annular sleeve is provided with an annular air bag groove, an air bag sleeve is sleeved in the annular air bag groove, the top of the inner wall of the blanking cavity is provided with a mounting groove, and the top of the inner wall of the mounting groove is fixedly connected with an electric push rod, the free end fixedly connected with small-size motor of electric putter, small-size motor's output fixedly connected with cover, the mounting groove with the station is supplied with to the bottle plug runs through and is provided with the air pressure cover, one side of air pressure cover pass through the air current pipe with annular gasbag groove intercommunication, air current pipe inner chamber right side cover is equipped with the sealing plug, the sealing plug right side articulates there is the connecting rod, movable groove has been seted up to mounting groove inner wall one side, movable groove cover is equipped with the extrusion piece, the extrusion piece with the connecting rod is articulated.

When blanking, respectively stacking a bottle body blanking groove station and a bottle plug supply station in sequence for placing a filling bottle and a bottle cap for the filling bottle, when an angle motor rotates for a fixed angle, when the filling bottle is filled with culture solution and a filling product moves to the whole lower part of the bottle plug supply station, after the top of a rotating circular table is separated from the bottom of a falling cavity in an overlapping way, the filling bottle lower than a bottle clamping groove moves to the right lower part of the falling cavity, at this time, the bottle cap falls from the falling cavity to the upper part of the filling bottle in the bottle clamping groove, at this time, the free end of an electric push rod is started to drive a cover sleeve to move downwards, the cover sleeve is contacted with the bottle cap on the filling bottle in the downward process of the cover sleeve, the bottle cap is stably sleeved on the opening of the filling bottle, at this time, the output end of a small-sized motor rotates, the cover sleeve drives the bottle cap to rotate, so that the bottle cap can be sealed with the filling bottle, and in the above process, when the cover sleeve moves downwards, after the cover contacts with the extrusion block, the extrusion block moves to the movable groove, the extrusion block drives the connecting rod to extrude the sealing plug, the sealing plug can drive the air pressure in the air pressure sleeve to enter the shape air bag groove from the air flow pipe, the air in the shape air bag groove increases at the moment, the air bag cover in the shape air bag groove is expanded by the air pressure, the bottle cover in the shape air bag groove is enabled to receive the pressure of the air bag cover, the air bag cover clamps the bottle cover tightly, therefore, in the sealing process of the bottle cap, the bottle cap at the lowest part is limited, when the cover moves to the highest part, the cover is separated from the contact back with the extrusion block, the air bag cover is recycled into the air pressure sleeve, therefore, the bottle cover is separated from the clamp with the bottle cap at the lowest part in the air pressure sleeve, the bottle cap falls into the falling cavity, in the reciprocating process, a plurality of filling bottles can be simultaneously sealed and operated, and the bottle packing is very fast.

Preferably, the pressurizing extraction mechanism comprises a pressure sleeve, a pressurizing sleeve is sleeved at the bottom of the pressure sleeve, a hydraulic telescopic cylinder is fixedly connected to the top of the pressure sleeve, a push rod is fixedly connected to the output end of the hydraulic telescopic cylinder, the bottom end of the push rod extends into the pressurizing sleeve and is fixedly connected with a pressurizing plate, a fixing plate is sleeved on the push rod, a plurality of transverse grooves are formed in the side wall of an inner cavity of the fixing plate at equal intervals, transmission wheels are movably connected to the left side and the right side of the inner cavity of each transverse groove, a transmission chain is sleeved on the two transmission wheels, a lifting gear is fixedly connected to one side of each transmission wheel, an outer gear sleeve is sleeved on the push rod and is meshed with the inner lifting gear, a lifting rack is arranged at the bottom of the fixing plate in an annular penetrating mode and is meshed with the outer lifting gear, a partition plate is sleeved in the middle of the pressure sleeve, the suction cover is run through to the equidistance on the baffle, the suction endotheca is equipped with the suction stopper, the top of suction stopper with promote rack fixed connection, the inner chamber intercommunication of suction cover has the extension pipe, a plurality of bracing pieces have been seted up to the individual distance of pressure jacket side surface, the bracing piece endotheca is equipped with communicating pipe, communicating pipe with suction cover inner chamber intercommunication, the inlet end of communicating pipe is provided with one-way water valve, the outer end fixedly connected with ejection of compact valve of bracing piece, ejection of compact valve with communicating pipe intercommunication, the bottom intercommunication of ejection of compact valve has the notes stub bar, ejection of compact valve bottom is provided with two level gauges.

When the filling bottle moves to the position for filling liquid, the hydraulic telescopic cylinder drives the push rod to move downwards, the pressurizing plate below the push rod moves downwards in the pressurizing sleeve at the moment, gas in the pressurizing sleeve is injected into the culture solution storage box by the pressurizing plate, the air pressure in the culture solution storage box is increased at the moment, the liquid level in the culture solution storage box is raised at the moment, the culture solution in the culture solution storage box is injected into the pressure sleeve from the liquid inlet pipe, the liquid level of the culture solution in the pressure sleeve is raised, the extension pipe is positioned below the liquid level of the culture solution, when the duration in the culture solution storage box is reduced, the pressure balancing device increases the pressure to the interior of the culture solution storage box, so that the liquid level can enter the pressure sleeve from the liquid inlet pipe timely and quickly when the pressurizing plate moves downwards, the sensitivity of the liquid level rise is increased, and in the process, the outer gear sleeve on the push rod drives the transmission wheel to rotate, the conveying chain can make the promotion gear rotation of transverse groove on the transfer gear, this makes the promotion gear in the outside drive and promotes rack rebound, the suction stopper of promotion rack bottom is rebound in the suction cover, the suction cover can be through extension tube suction culture solution this moment, when the interior culture solution of suction cover rises, the culture solution gets into in the communicating tube from one-way water valve, and fill in the filling bottle in from the bleeder valve, when the liquid in the filling bottle reaches the liquid level requirement, the level gauge can detect out liquid level height, and control one-way water valve and close.

Preferably, the feeding rotating mechanism comprises a rotating round platform, a bottle clamping groove with the same number as that of stations of the bottle body blanking groove is formed in the side surface of the rotating round platform, two inner extension grooves are formed in the side surface of the rotating round platform, the guide block is positioned in the inner extension grooves, a square groove is formed in one side of the inner wall of the bottle clamping groove, two slide bars are fixedly connected in the square groove, a movable block is sleeved on the two slide bars, one side of the movable block is elastically connected to one side of the inner wall of the square groove through an extrusion spring, an extrusion rod is fixedly connected to one side of the movable block, a hinge block is fixedly connected to the outer end of the compression rod, an arc-shaped block is fixedly connected to one side of the hinge block, clamping plates are hinged to two sides of the arc-shaped block, a sponge plate is fixedly connected to one side of the inner wall of the clamping plate, and an axle seat is fixedly connected to the outer wall of the clamping plate, the rotating shaft is connected with a rotating ring in a rotating mode, an arc-shaped rod is fixedly connected to one side of the rotating ring, an elastic sleeve is fixedly connected to one side of the extrusion rod, an elastic groove is formed in the elastic sleeve and is elastically connected with a sliding block through a reset spring, and the sliding block is hinged to the arc-shaped rod.

Through the use of mutually supporting two chucking boards and arc, when the filling bottle falls between two chucking boards and arc, and when rotating the round platform and rotate, utilize the effect of guide block, the filling bottle can be to the sponge board contact, and make two chucking boards open to both sides, but because two chucking boards elastic rotation, after the filling bottle is complete with the sponge board contact, under reset spring's elastic action, two chucking boards and arc can live the filling bottle parcel, can increase the stability that the filling bottle removed like this, and can guarantee that the filling bottle is in the position of filling point all the time when the guide block department at every turn, guarantee the stability of jar liquid, the problem of avoiding appearing liquid and overflowing appears.

When the rotary circular table rotates a certain angle, filling bottles fall into the bottle clamping grooves in each bottle body blanking groove station, the filling bottles are located between the two clamping plates and the arc-shaped blocks and are in contact with the sponge plates at the moment, the angle motor continues to rotate the communicated angle, the surfaces of the filling bottles between the two clamping plates and the arc-shaped blocks are in contact with the outer walls of the guide blocks at the moment, in the process of rotating the circular table, the arc-shaped protruding parts of the guide blocks extrude the filling bottles more and more obviously, the filling bottles can extrude the two clamping plates and the arc-shaped blocks towards the axis of the rotary circular table, the extrusion rods move towards the inside of the square groove, meanwhile, the movable blocks move on the two slide rods and extrude the extrusion springs, the filling bottles move towards the inner side of the inner wall of the bottle clamping grooves, and at the moment, the filling bottles move to the positions of filling liquid.

Preferably, recovery unit includes the ring gear, the ring gear cover is established fixed bolster is sheathe in, the cover is equipped with conveyer on the ring gear, the vertical hair layer of ring gear top fixedly connected with, the cover is equipped with annular conveying cover on the fixed bolster, the piece is right to the equidistant fixedly connected with spiral of annular conveying cover inner wall, annular conveying cover passes through limit baffle fixed connection dustcoat, row's silo has all been seted up to the left and right sides of dustcoat, row's silo endotheca is equipped with the unloading cover, unloading cover inner wall bottom fixedly connected with unloading cloth cover, the both sides fixedly connected with arc limiting plate of unloading cloth cover.

When the angle motor continues to rotate, when the rotary round platform rotates a certain angle, sealed filling bottle moves to unloading station department this moment, the filling bottle descends from the unloading station and descends from spiral centralizer piece, when filling bottle bottom falls to the top of erecting the hair layer, driving motor drives the ring pulley through conveyer and rotates, the ring pulley makes and erects the hair layer and rotate, erect the hair layer and drive the filling bottle and stand under the effect of spiral centralizer piece, along with erecting the rotation of hair layer, when filling bottle and stopper contact, the stopper can drive the filling bottle to unloading cover department, and fall in the collection box in the unloading cloth cover, can guarantee like this that the filling bottle is in vertical state all the time, and then guarantee the stability of filling bottle conveying.

Preferably, the one-way air outlet valve comprises a square frame, the square frame is elastically connected with a rotating shaft at equal intervals, baffles are sleeved on the rotating shaft, and two adjacent baffles are in V-shaped contact.

Therefore, when the pressure increasing plate moves downwards, the air flow inside the pressure increasing plate can enter the culture solution storage box, and when the pressure increasing plate moves upwards, the baffle is closed in a V shape, so that the pressure leakage of the culture solution storage box is reduced.

Preferably, the extraction method of the biological immune cell culture solution extraction system comprises the following steps:

the first step, when the rotary round platform rotates a certain angle, the filling bottle in each bottle body blanking groove station falls into the bottle clamping groove, at the moment, the filling bottle is positioned between the two clamping plates and the arc-shaped block and contacts with the sponge plate, the angle motor continues to rotate the communicated angle, at the moment, the surface of the filling bottle between the two clamping plates and the arc-shaped block contacts with the outer wall of the guide block, in the process of rotating the round platform, the arc-shaped bulge part of the guide block extrudes the filling bottle more and more obviously, the filling bottle can extrude the two clamping plates and the arc-shaped block towards the axis of the rotary round platform, so that the extrusion rod moves towards the inside of the square groove, at the same time, the movable block moves on the two slide bars and extrudes the extrusion spring, so that the filling bottle moves towards the inner side of the inner wall of the bottle clamping groove, at the moment, the filling bottle moves to the position of filling liquid, through the mutual matching of the two clamping plates and the arc-shaped block, when the filling bottle falls between the two clamping plates and the arc-shaped block and the rotary circular table rotates, the filling bottle can contact the sponge plate by utilizing the action of the guide block and can be opened towards two sides, and the two clamping plates can elastically rotate;

secondly, when the filling bottle moves to the position for filling liquid, the hydraulic telescopic cylinder drives the push rod to move downwards, the pressurizing plate below the push rod moves downwards in the pressurizing sleeve at the moment, gas in the pressurizing sleeve is injected into the culture solution storage box by the pressurizing plate, the air pressure in the culture solution storage box is increased at the moment, the liquid level in the culture solution storage box is raised at the moment, the culture solution in the culture solution storage box is injected into the pressure sleeve from the liquid inlet pipe, the liquid level of the culture solution in the pressure sleeve rises and the extension pipe is positioned below the liquid level of the culture solution, when the duration of the interior of the culture solution storage box is reduced, the pressure balancing device increases the pressure to the interior of the culture solution storage box, so that the liquid level can enter the pressure sleeve from the liquid inlet pipe timely and quickly when the pressurizing plate moves downwards, the sensitivity of the rise of the liquid level is increased, and the outer gear sleeve on the push rod drives the transmission wheel to rotate, the conveying chain on the conveying wheel can enable the lifting gear of the transverse groove to rotate, so that the lifting gear on the outer side drives the lifting rack to move upwards, the suction plug at the bottom end of the lifting rack moves upwards in the suction sleeve, the suction sleeve can suck culture fluid through the extension pipe at the moment, when the culture fluid in the suction sleeve rises, the culture fluid enters the communication pipe from the one-way water valve and is filled in the filling bottle from the discharge valve, and when the liquid in the filling bottle meets the liquid level requirement, the liquid level meter can detect the liquid level height and control the one-way water valve to be closed;

a third step; when blanking, respectively stacking a bottle body blanking groove station and a bottle plug supply station in sequence for placing a filling bottle and a bottle cap for the filling bottle, when an angle motor rotates for a fixed angle, when the filling bottle is filled with culture solution and a filling product moves to the whole lower part of the bottle plug supply station, after the top of a rotating circular table is separated from the bottom of a falling cavity in an overlapping way, the filling bottle lower than a bottle clamping groove moves to the right lower part of the falling cavity, at this time, the bottle cap falls from the falling cavity to the upper part of the filling bottle in the bottle clamping groove, at this time, the free end of an electric push rod is started to drive a cover sleeve to move downwards, the cover sleeve is contacted with the bottle cap on the filling bottle in the downward process of the cover sleeve, the bottle cap is stably sleeved on the opening of the filling bottle, at this time, the output end of a small-sized motor rotates, the cover sleeve drives the bottle cap to rotate, so that the bottle cap can be sealed with the filling bottle, and in the above process, when the cover sleeve moves downwards, after the cover sleeve is contacted with the extrusion block, the extrusion block moves towards the movable groove, the extrusion block drives the connecting rod to extrude the sealing plug, the sealing plug can drive air pressure in the air pressure sleeve to enter the shaped air bag groove from the air flow pipe, the air in the shaped air bag groove is increased, the air bag sleeve in the shaped air bag groove is expanded by the air pressure, so that the bottle cover positioned in the shaped air bag groove is pressed by the air bag sleeve, the air bag sleeve clamps the bottle cover, the lowermost bottle cap is limited in the process of sealing the bottle cap, and when the cover sleeve moves to the top, the cover sleeve is separated from the extrusion block and then is retracted into the air pressure sleeve, so that the air bag sleeve is separated from the lowermost bottle cap in the air pressure sleeve to be clamped, the bottle cover falls into the falling cavity, and in the reciprocating process, a plurality of filling bottles can be simultaneously sealed and are very fast;

the fourth step; when the angle motor continues to rotate, when the rotary round platform rotates a certain angle, sealed filling bottle moves to unloading station department this moment, the filling bottle descends from the unloading station and descends from spiral centralizer piece, when filling bottle bottom falls to the top of erecting the hair layer, driving motor drives the ring pulley through conveyer and rotates, the ring pulley makes and erects the hair layer and rotate, erect the hair layer and drive the filling bottle and stand under the effect of spiral centralizer piece, along with erecting the rotation of hair layer, when filling bottle and stopper contact, the stopper can drive the filling bottle to unloading cover department, and fall in the collection box in the unloading cloth cover, can guarantee like this that the filling bottle is in vertical state all the time, and then guarantee the stability of filling bottle conveying.

The invention has at least the following beneficial effects:

1. the invention uses the bottling supply mechanism, when the bottle body blanking groove station and the bottle plug supply station are respectively stacked and placed with the filling bottle and the bottle cap for the filling bottle in sequence, when the angle motor rotates for a fixed angle, when the filling bottle is filled with culture solution, the filling product moves to the whole lower part of the bottle plug supply station, the top of the rotating circular table is separated from the lap joint with the bottom of the falling cavity, the filling bottle lower than the bottle clamping groove moves to the right lower part of the falling cavity, at this time, the bottle cap falls on the filling bottle in the bottle clamping groove from the falling cavity, at this time, the free end of the electric push rod is started to drive the cover sleeve to move downwards, the cover sleeve and the bottle cap on the filling bottle are contacted in the downward process of the cover sleeve, the bottle cap is stably sleeved on the bottle opening of the filling bottle, at this time, the output end of the small motor rotates, the cover sleeve drives the bottle cap to rotate, so that the bottle cap can be sealed with the filling bottle, when the in-process that the cover moved down, the cover contacts the back with the extrusion piece, the extrusion piece removes to the movable slot, the extrusion piece drives connecting rod extrusion sealing plug, the sealing plug can drive the atmospheric pressure in the atmospheric pressure cover and follow the intraductal shape gasbag inslot that gets into of air current this moment, the gaseous increase in shape gasbag inslot this moment, the gasbag cover in shape gasbag inslot receives atmospheric pressure inflation, make the bottle lid that is located shape gasbag groove receive the pressure of gasbag cover, gasbag cover presss from both sides the bottle lid tightly like this, consequently at the sealed in-process of bottle cap, the bottle cap of below is restricted.

2. In order to accurately fill culture solution, when a filling bottle moves to a position for filling liquid, a hydraulic telescopic cylinder drives a push rod to move downwards, a pressurizing plate below the push rod moves downwards in a pressurizing sleeve, gas in the pressurizing sleeve is injected into a culture solution storage box by the pressurizing plate, the air pressure in the culture solution storage box is increased, the liquid level in the culture solution storage box is raised, the culture solution in the culture solution storage box is injected into the pressure sleeve from a liquid inlet pipe, the liquid level of the culture solution in the pressure sleeve is raised, an extension pipe is positioned below the liquid level of the culture solution, when the inner pressure of the culture solution storage box is reduced, a pressure balancing device increases the pressure to the inner part of the culture solution storage box, so that the liquid level can enter the pressure sleeve from the liquid inlet pipe timely and quickly when the pressurizing plate moves downwards, and the sensitivity of the liquid level rising is increased, in this process, outer tooth cover on the push rod drives the transfer gear and rotates, the last transfer chain of transfer gear can make the promotion gear rotation of transverse groove, this makes the promotion gear in the outside drive the promotion rack rebound, the suction plug that promotes the rack bottom is at the rebound in the suction cover, the suction cover can be through extension tube suction culture solution this moment, when the culture solution rises in the suction cover, the culture solution gets into in the intercommunication pipe from one-way water valve, and from the filling in the bleeder valve in the filling bottle, when the liquid in the filling bottle reaches the liquid level requirement, the level gauge can detect out liquid level, and control one-way water valve and close.

3. According to the invention, through the mutually matched use of the two clamping plates and the arc-shaped block, when a filling bottle falls between the two clamping plates and the arc-shaped block and the rotary circular table rotates, the filling bottle can contact the sponge plate under the action of the guide block and can be opened towards two sides, and the two clamping plates can elastically rotate, so that after the filling bottle is completely contacted with the sponge plate, the filling bottle can be wrapped by the two clamping plates and the arc-shaped block under the elastic action of the reset spring, the moving stability of the filling bottle can be increased, and the filling bottle can be ensured to be always positioned at a filling point when passing through the guide block every time, the stability of can liquid is ensured, and the problem of liquid overflow is avoided.

4. According to the invention, through the use of the recovery device, when the angle motor continues to rotate, the rotary circular table rotates for a certain angle, and at the moment, the sealed filling bottles move to the blanking station, the filling bottles fall from the blanking station and slide down from the spiral centering block, when the bottoms of the filling bottles fall to the top of the vertical feather layers, the driving motor drives the annular wheel to rotate through the conveying belt, the annular wheel enables the vertical feather layers to rotate, the vertical feather layers drive the filling bottles to stand up under the action of the spiral centering block, and along with the rotation of the vertical feather layers, when the filling bottles are in contact with the limiting blocks, the limiting blocks can drive the filling bottles to the blanking sleeve and fall into the recovery box from the inside of the blanking cloth sleeve, so that the filling bottles can be ensured to be in a vertical state all the time, and further the conveying stability of the filling bottles is ensured.

5. The invention can simultaneously carry out liquid filling operation on a plurality of filling bottles, and after the filling is finished, the filled filling bottles are capped under the action of the angle motor, the pre-filling is carried out as usual in the capping process, and the sealed filling bottles are collected after being transmitted under the action of the angle motor.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:

FIG. 1 is an overall front cross-sectional view of the present invention;

FIG. 2 is a top view of the present invention as a whole;

FIG. 3 is a view showing the structure at A in FIG. 1;

FIG. 4 is a view of the structure at C in FIG. 1;

FIG. 5 is a view showing a structure at B in FIG. 1;

FIG. 6 is a cross-sectional view of a side view of the annular blanking mechanism of the present invention;

FIG. 7 is a partial cross-sectional view of a top view of the rotary turret of the present invention.

In the figure, 1 base, 2 culture solution storage box, 3 pressurizing air pump, 4 pressure balancing device, 5 liquid inlet pipe, 6 one-way gas outlet valve, 7 servo motor, 8 toothed shaft, 9 annular toothed groove, 10 rotating sleeve, 11 fixed sleeve, 12 pressurizing extraction mechanism, 121 pressure sleeve, 122 pressurizing sleeve, 123 hydraulic telescopic cylinder, 124 fixed plate, 125 push rod, 1251 pressurizing plate, 126 external toothed sleeve, 127 transverse groove, 128 transmission wheel, 129, 130 pumping sleeve, 131 lifting rack, 132 pumping plug, 133 one-way water valve, 134 extending pipe, 135 supporting rod, 136 communicating pipe, 137 discharging valve, 138 injecting head, 139 liquidometer, 13 transmission belt, 14 charging rotating mechanism, 1401 bottle clamping groove, 141 rotating round table, 142 square groove, 143 sliding rod, 144 movable block, 145 extrusion spring, 146 elastic sleeve, 147 elastic groove, 148 return spring, 149 sliding block, 150 extrusion rod, 151 hinged block, 152 arc block, 153 clamping plates, 154 sponge plates, 155 shaft seats, 156 swivel rings, 157 arc-shaped rods, 15 fixed supporting sleeves, 16 bottling supply mechanisms, 161 annular rails, 162 annular blanking mechanisms, 163 bottle blanking groove stations, 164 blanking stations, 165 guide blocks, 166 bottle stopper supply stations, 167 annular sleeves, 168 annular air bag grooves, 169 air bag sleeves, 170 falling cavities, 171 mounting grooves, 172 cover sleeves, 173 small motors, 174 electric push rods, 175 air pressure sleeves, 176 airflow pipes, 177 sealing plugs, 178 movable grooves, 179 extrusion blocks, 17 driving motors, 18 recovery devices, 181 annular wheels, 182 annular conveying sleeves, 183 spiral centering blocks, 184 outer covers, 185 vertical hair layers, 186 discharge grooves, 187 blanking sleeves, 188 blanking cloth sleeves, 189 arc-shaped limiting plates, 19 limiting baffles and 20 recovery boxes.

Detailed Description

Embodiments of the present application will be described in detail with reference to the drawings and examples, so that how to implement technical means to solve technical problems and achieve technical effects of the present application can be fully understood and implemented.

As shown in fig. 1 to 7, the present invention provides a biological immune cell culture fluid extraction system, comprising: the culture medium extraction device comprises a base 1, a culture medium storage box 2 is fixedly connected to the middle of the top of the base 1, a pressurizing air pump 3 is fixedly connected to the right side of the top of the culture medium storage box 2, a pressure balancing device 4 is fixedly connected to the right side of the culture medium storage box 2, a pressurizing extraction mechanism 12 is fixedly communicated with the top of the culture medium storage box 2, a driving motor 17 is fixedly connected to the right side of the top of the base 1, a driving wheel is fixedly connected to the output end of the driving motor 17, a one-way air outlet valve 6 is sleeved below the inner cavity of the pressurizing extraction mechanism 12 and connected with the culture medium storage box 2, a one-way air inlet valve is sleeved on the front side of the inner wall of the pressurizing extraction mechanism 12, two angle motors 7 are fixedly connected to the lower side of the pressurizing extraction mechanism 12, a gear shaft 8 is fixedly connected to the output end of each angle motor 7, a rotating sleeve 10 is sleeved on the middle of the pressurizing extraction mechanism 12, a fixing sleeve 11 is sleeved on the rotating sleeve 10, and an annular gear slot 9 is arranged below the side of the

fixing sleeve

11, 8 meshing of tooth axle are in annular tooth's socket 9, mechanism 12 inner chamber middle part intercommunication is drawed in the pressure boost has two feed liquor pipes 5, the bottom of feed liquor pipe 5 runs through the top of culture solution storage box 2 and extends to the below of inner chamber, the pressure boost is drawed on mechanism 12 and is located the top cover of fixed cover 11 and be equipped with reinforced slewing mechanism 14, the top fixed connection at fixed cover 11 of the bottom fixed connection of reinforced slewing mechanism 14, base 1 top fixed connection has fixed support cover 15, fixed support cover 15 top fixed connection has bottling feed mechanism 16, the cover is equipped with recovery unit 18 on the fixed support cover 15, the drive wheel passes through conveyer 13 and is connected with the transmission of recovery unit 18, both sides all are provided with limit baffle 19 around bottling feed mechanism 16, just be located recovery unit 18 below on the base 1 and be provided with collection box 20.

The bottling supply mechanism 16 comprises an annular rail 161, an annular blanking mechanism 162 is fixedly connected to the annular rail 161, the top of the annular blanking mechanism 162 is communicated with a plurality of bottle body blanking groove stations 163, a bottle stopper supply station 166 is arranged at the top of the annular rail 161 and is positioned at the bottle body blanking groove station 163, a blanking station 164 is arranged at the top of the inner wall of the annular rail 161 and is separated from the bottle body blanking groove station 163 and the bottle stopper supply station 166, two guide blocks 165 are arranged between the bottle body blanking groove station 163 and the bottle stopper supply station 166, an inclined cavity is arranged at the bottom of the inner cavity of the bottle stopper supply station 166 and is communicated with the blanking cavity 170, an annular sleeve 167 is sleeved below the inner cavity of the bottle stopper supply station 166, an annular air bag groove 168 is arranged on the inner wall of the annular sleeve 167, an air bag sleeve 169 is sleeved in the annular air bag groove 168, a mounting groove 171 is arranged at the top of the inner wall of the blanking cavity 170, and an electric push rod 174 is fixedly connected to the top of the inner wall of the mounting groove 171, electric putter 174's free end fixedly connected with small motor 173, small motor 173's output fixedly connected with lid cover 172, mounting groove 171 runs through with bottle plug supply station 166 and is provided with air pressure cover 175, one side of air pressure cover 175 is passed through air current pipe 176 and annular gasbag groove 168 intercommunication, air current pipe 176 inner chamber right side cover is equipped with sealing plug 177, the sealing plug 177 right side articulates there is the connecting rod, movable groove 178 has been seted up to mounting groove 171 inner wall one side, movable groove 178 cover is equipped with extrusion piece 179, extrusion piece 179 is articulated with the connecting rod.

When blanking, the bottle body blanking slot station 163 and the bottle stopper supply station 166 are respectively stacked and placed with a filling bottle and a bottle cap for the filling bottle, when the angle motor 7 rotates for a fixed angle, when the filling bottle is filled with culture solution, and the filling product moves to the whole lower part of the bottle stopper supply station 166, after the top of the rotary circular platform 140 is separated from the bottom of the falling cavity 170, the filling bottle lower than the bottle clamping slot 1401 moves to the right lower part of the falling cavity 170, at this time, the bottle cap falls from the falling cavity 170 to the upper part of the filling bottle in the bottle clamping slot 1401, at this time, the free end of the electric push rod 174 is started to drive the cover sleeve 172 to move downwards, the cover sleeve 172 and the bottle cap on the filling bottle are contacted in the downward process of the cover sleeve 172, the bottle cap is stably sleeved on the mouth of the filling bottle, at this time, the output end of the small motor 173 rotates, the cover sleeve 172 drives the bottle cap to rotate, so that the bottle cap can be sealed with the filling bottle, in the above process, when the cover 172 moves downwards, after the cover 172 contacts the extrusion block 179, the extrusion block 179 moves into the movable groove 178, the extrusion block 179 drives the connecting rod to extrude the sealing plug 177, at this time, the sealing plug 177 can drive the air pressure in the air pressure sleeve 175 to enter the air bag groove 168 from the air flow pipe 176, at this time, the air in the air bag groove 168 increases, the air bag sleeve 169 in the air bag groove 168 is inflated by the air pressure, so that the bottle cap located in the air bag groove 168 is pressed by the air bag sleeve 169, so that the air bag sleeve 169 clamps the bottle cap, therefore, during the sealing of the bottle cap, the lowermost bottle cap is limited, and when the cover 172 moves to the uppermost, after the cover 172 is separated from the extrusion block 179, the air bag sleeve 169 retracts into the air pressure sleeve 175, so that the air pressure sleeve 175 is separated from the lowermost bottle cap, the bottle cap falls into the falling chamber 170, and thus, during the reciprocating process, can a plurality of filling bottles simultaneously seal operation, unusual quick.

The pressurizing extraction mechanism 12 comprises a pressure sleeve 121, a pressurizing sleeve 122 is sleeved at the bottom of the pressure sleeve 121, a hydraulic telescopic cylinder 123 is fixedly connected to the top of the pressure sleeve 121, a push rod 125 is fixedly connected to the output end of the hydraulic telescopic cylinder 123, the bottom end of the push rod 125 extends into the pressurizing sleeve 122 and is fixedly connected with a pressurizing plate 1251, a fixing plate 124 is sleeved on the push rod 125, a plurality of transverse grooves 127 are formed in the side wall of an inner cavity of the fixing plate 124 at equal intervals, transmission wheels 128 are movably connected to the left side and the right side of the inner cavity of the transverse grooves 127, transmission chains are sleeved on the two transmission wheels 128, a lifting gear 129 is fixedly connected to one side of each transmission wheel 128, an outer gear sleeve 126 is sleeved on the push rod 125, the outer gear sleeve 126 is meshed with a lifting gear 129 on the inner side, a lifting rack 131 is arranged at the bottom of the fixing plate 124 in an annular penetrating manner, the lifting rack 131 is meshed with the lifting gear 129 on the outer side, a partition plate is sleeved on the middle of the pressure sleeve 121, and penetrates through the sucking sleeve 130 at equal intervals, suction plug 132 is equipped with to suction cover 130 endotheca, suction plug 132's top and lifting rack 131 fixed connection, suction cover 130's inner chamber intercommunication has extension 134, a plurality of bracing pieces 135 have been seted up to the distance that pressure cover 121 side surface is individual, bracing piece 135 endotheca is equipped with communicating pipe 136, communicating pipe 136 and suction cover 130 inner chamber intercommunication, the inlet end of communicating pipe 136 is provided with one-way water valve 133, bracing piece 135's outer end fixedly connected with ejection of compact valve 137, ejection of compact valve 137 and communicating pipe 136 intercommunication, ejection of compact valve 137's bottom intercommunication has annotate stub bar 138, ejection of compact valve 137 bottom is provided with two level gauges 139.

When the filling bottle moves to the position for filling liquid, the hydraulic telescopic cylinder 123 drives the push rod 125 to move downwards, at this time, the pressurizing plate 1251 below the push rod 125 moves downwards in the pressurizing sleeve 122, the gas in the pressurizing sleeve 122 is injected into the culture solution storage box 2 by the pressurizing plate 1251, at this time, the air pressure inside the culture solution storage box 2 is increased, at this time, the liquid level inside the culture solution storage box 2 is raised, the culture solution inside the culture solution storage box 2 is injected into the pressure sleeve 121 from the liquid inlet pipe 5, so that the liquid level of the culture solution is raised in the pressure sleeve 121 and the extension pipe 134 is positioned below the liquid level of the culture solution, and when the interior of the culture solution storage box 2 is reduced, the pressure balancing device 4 increases the pressure inside the culture solution storage box 2, so that the liquid level can timely and quickly enter the pressure sleeve 121 from the liquid inlet pipe 5 when the pressurizing plate 1251 moves downwards, the sensitivity of the liquid level is increased, in the process, the outer gear sleeve 126 on the push rod 125 drives the transmission wheel 128 to rotate, the transmission chain on the transmission wheel 128 can enable the lifting gear 129 of the transverse groove 127 to rotate, the lifting gear 129 on the outer side drives the lifting rack 131 to move upwards, the suction plug 132 at the bottom end of the lifting rack 131 moves upwards in the suction sleeve 130, at the moment, the suction sleeve 130 can suck culture liquid through the extension pipe 134, when the culture liquid in the suction sleeve 130 rises, the culture liquid enters the communication pipe 136 from the one-way water valve 133 and is filled in the filling bottle from the discharge valve 137, and when the liquid in the filling bottle meets the liquid level requirement, the liquid level meter 139 can detect the liquid level and control the one-way water valve 133 to close.

The feeding rotating mechanism 14 comprises a rotating circular table 141, the side surface of the rotating circular table 141 is provided with bottle clamping grooves 1401 the number of which is the same as that of bottle body blanking groove stations 163, the side surface of the rotating circular table 141 is provided with two inner extending grooves, a guide block 165 is positioned in the inner extending grooves, one side of the inner wall of the bottle clamping groove 1401 is provided with a square groove 142, two sliding rods 143 are fixedly connected in the square groove 142, two sliding rods 143 are sleeved with a movable block 144, one side of the movable block 144 is elastically connected to one side of the inner wall of the square groove 142 through an extrusion spring 145, one side of the movable block 144 is fixedly connected with an extrusion rod 150, the outer end of the compression rod 150 is fixedly connected with a hinged block 151, one side of the hinged block 151 is fixedly connected with an arc block 152, two sides of the arc block 152 are hinged with clamping plates 153, one side of the inner wall of the clamping plate 153 is fixedly connected with a sponge plate 154, the outer wall of the clamping plate 153 is fixedly connected with a shaft seat 155, and the shaft seat 155 is rotatably connected with a rotating ring 156, an arc-shaped rod 157 is fixedly connected to one side of the rotating ring 156, an elastic sleeve 146 is fixedly connected to one side of the extrusion rod 150, an elastic groove 147 is arranged in the elastic sleeve 146, the elastic groove 147 is elastically connected with a sliding block 149 through a return spring 148, and the sliding block 149 is hinged to the arc-shaped rod 157.

Through the use of mutually supporting to two chucking plates 153 and arc piece 152, when the filling bottle falls between two chucking plates 153 and arc piece 152, and when rotating round platform 141 and rotate, utilize guide block 165's effect, the filling bottle can be to sponge 154 contact, and make two chucking plates 153 open to both sides, because but two chucking plates 153 elastic rotation, after the filling bottle is complete with sponge 154 contact, under reset spring 148's elastic action, two chucking plates 153 and arc piece 152 can live the filling bottle parcel, can increase the stability that the filling bottle removed like this, and can guarantee that the filling bottle is located the position of filling point all the time when the filling bottle passes through guide block 165 department at every turn, guarantee the stability of jar liquid, the problem of avoiding appearing liquid and overflowing appears.

When the rotary circular table 141 rotates for a certain angle, the filling bottles in each bottle body blanking slot station 163 fall into the bottle clamping slot 1401, the filling bottles are positioned between the two clamping plates 153 and the arc-shaped block 152 and are contacted with the sponge plate 154 at the moment, the angle motor 7 continues to rotate for a communicated angle, the surfaces of the filling bottles between the two clamping plates 153 and the arc-shaped block 152 are contacted with the outer wall of the guide block 165 at the moment, in the process of rotating the circular truncated cone 141, the arc-shaped protruding part of the guide block 165 extrudes the filling bottle more and more obviously, the filling bottle can extrude the two clamping plates 153 and the arc-shaped block 152 to the axis of the rotating circular truncated cone 141, thus, the pressing bar 150 moves toward the inside of the square groove 142, and at the same time, the movable block 144 moves on the two slide bars 143, and presses the pressing spring 145 so that the filling bottle moves to the inside of the inner wall of the bottle catching groove 1401, at which time the filling bottle moves to a position where the liquid is filled.

Recovery unit 18 includes ring gear 181, the ring gear 181 cover is established on fixed support cover 15, the cover is equipped with conveyer 13 on the ring gear 181, ring gear 181 top fixedly connected with erects hair layer 185, the cover is equipped with annular conveying cover 182 on the fixed support cover 15, equidistant fixedly connected with spiral righting block 183 of annular conveying cover 182 inner wall, annular conveying cover 182 passes through limit baffle 19 fixed connection dustcoat 184, row's silo 186 has all been seted up to the left and right sides of dustcoat 184, row's silo 186 endotheca is equipped with unloading cover 187, unloading cover 187 inner wall bottom fixedly connected with unloading cloth cover 188, the both sides fixedly connected with arc limiting plate 189 of unloading cloth cover 188.

When the angle motor 7 continues to rotate, the rotary circular truncated cone 141 rotates by a certain angle, and at the moment, the sealed filling bottles move to the blanking station 164, the filling bottles fall from the blanking station 164 and slide down from the spiral centering block 183, when the bottoms of the filling bottles fall to the top of the vertical feather layers 185, the driving motor 17 drives the annular wheel 181 to rotate through the conveying belt 13, the annular wheel 181 enables the vertical feather layers 185 to rotate, the vertical feather layers 185 drive the filling bottles to stand under the action of the spiral centering block 183, and along with the rotation of the vertical feather layers 185, when the filling bottles are in contact with the limiting block 19, the limiting block 19 can drive the filling bottles to the blanking sleeve 187 and fall into the recovery box 20 from the inside of the blanking cloth sleeve 188, so that the filling bottles can be ensured to be in a vertical state all the time, and the conveying stability of the filling bottles is ensured.

The one-way air outlet valve 6 comprises a square frame, the square frame is elastically connected with a rotating shaft at equal intervals, baffles are sleeved on the rotating shaft, and two adjacent baffles are in V-shaped contact.

Thus, when the pressurizing plate 1251 moves downward, the air flow inside can enter the culture solution storage tank 2, and when the pressurizing plate 1251 moves upward, the baffle is closed in a V-shape, thereby reducing the pressure leakage of the culture solution storage tank 2.

An extraction method of a biological immune cell culture solution extraction system comprises the following steps:

first, when the rotary circular table 141 rotates a certain angle, the filling bottle in each bottle feeding station 163 falls into the bottle clamping groove 1401, the filling bottle is positioned between the two clamping plates 153 and the arc-shaped block 152 and contacts with the sponge plate 154, the angle motor 7 continues to rotate by a communicated angle, the surface of the filling bottle between the two clamping plates 153 and the arc-shaped block 152 contacts with the outer wall of the guide block 165, the arc-shaped bulge part of the guide block 165 extrudes the filling bottle more and more obviously in the process of rotating the circular table 141, the filling bottle can extrude the two clamping plates 153 and the arc-shaped block 152 towards the axis of the rotary circular table 141, so that the extruding rod 150 moves towards the inside of the square groove 142, meanwhile, the movable block 144 moves on the two sliding rods 143 and extrudes the extruding spring 145, so that the filling bottle moves towards the inner side of the inner wall of the bottle clamping groove 1401, and at the moment, the filling bottle moves to the position of filling liquid, through the use of the two clamping plates 153 and the arc-shaped blocks 152 which are matched with each other, when filling bottles fall between the two clamping plates 153 and the arc-shaped blocks 152, and when the rotary circular truncated cone 141 rotates, the filling bottles can contact the sponge plates 154 by using the action of the guide blocks 165, and the two clamping plates 153 are opened towards two sides, because the two clamping plates 153 can elastically rotate, after the filling bottles completely contact the sponge plates 154, the filling bottles can be wrapped by the two clamping plates 153 and the arc-shaped blocks 152 under the elastic action of the reset springs 148, so that the moving stability of the filling bottles can be increased, and the filling bottles can be ensured to be always positioned at filling points when passing through the guide blocks 165 every time, the stability of can be ensured, and the problem of liquid overflow can be avoided;

secondly, when the filling bottle moves to the position for filling liquid, the hydraulic telescopic cylinder 123 drives the push rod 125 to move downwards, at this time, the pressurizing plate 1251 below the push rod 125 moves downwards in the pressurizing sleeve 122, and the gas in the pressurizing sleeve 122 is injected into the culture solution storage box 2 by the pressurizing plate 1251, at this time, the air pressure inside the culture solution storage box 2 increases, at this time, the liquid level inside the culture solution storage box 2 rises, the culture solution inside the culture solution storage box 2 is injected into the pressure sleeve 121 from the liquid inlet pipe 5, so that the liquid level of the culture solution rises in the pressure sleeve 121 and the extension pipe 134 is positioned below the liquid level of the culture solution, and when the inside of the culture solution storage box 2 is reduced, the pressure balancing device 4 increases the pressure inside the culture solution storage box 2, so that the liquid level can enter the pressure sleeve 121 from the liquid inlet pipe 5 on time and quickly when the pressurizing plate 1251 moves downwards, the sensitivity of the liquid level rising is increased, in the process, the outer gear sleeve 126 on the push rod 125 drives the transmission wheel 128 to rotate, the transmission chain on the transmission wheel 128 can enable the lifting gear 129 of the transverse groove 127 to rotate, the outer lifting gear 129 drives the lifting rack 131 to move upwards, the suction plug 132 at the bottom end of the lifting rack 131 moves upwards in the suction sleeve 130, at the moment, the suction sleeve 130 can suck culture fluid through the extension pipe 134, when the culture fluid in the suction sleeve 130 rises, the culture fluid enters the communication pipe 136 from the one-way water valve 133 and is filled in the filling bottle from the discharge valve 137, and when the liquid in the filling bottle meets the liquid level requirement, the liquid level meter 139 can detect the liquid level and control the one-way water valve 133 to close;

a third step; when blanking, the bottle body blanking slot station 163 and the bottle stopper supply station 166 are respectively stacked and placed with a filling bottle and a bottle cap for the filling bottle, when the angle motor 7 rotates for a fixed angle, when the filling bottle is filled with culture solution, and the filling product moves to the whole lower part of the bottle stopper supply station 166, after the top of the rotary circular platform 140 is separated from the bottom of the falling cavity 170, the filling bottle lower than the bottle clamping slot 1401 moves to the right lower part of the falling cavity 170, at this time, the bottle cap falls from the falling cavity 170 to the upper part of the filling bottle in the bottle clamping slot 1401, at this time, the free end of the electric push rod 174 is started to drive the cover sleeve 172 to move downwards, the cover sleeve 172 and the bottle cap on the filling bottle are contacted in the downward process of the cover sleeve 172, the bottle cap is stably sleeved on the mouth of the filling bottle, at this time, the output end of the small motor 173 rotates, the cover sleeve 172 drives the bottle cap to rotate, so that the bottle cap can be sealed with the filling bottle, in the above process, when the cover 172 moves downwards, after the cover 172 contacts the extrusion block 179, the extrusion block 179 moves into the movable groove 178, the extrusion block 179 drives the connecting rod to extrude the sealing plug 177, at this time, the sealing plug 177 can drive the air pressure in the air pressure sleeve 175 to enter the air bag groove 168 from the air flow pipe 176, at this time, the air in the air bag groove 168 increases, the air bag sleeve 169 in the air bag groove 168 is inflated by the air pressure, so that the bottle cap located in the air bag groove 168 is pressed by the air bag sleeve 169, so that the air bag sleeve 169 clamps the bottle cap, therefore, during the sealing of the bottle cap, the lowermost bottle cap is limited, and when the cover 172 moves to the uppermost, after the cover 172 is separated from the extrusion block 179, the air bag sleeve 169 retracts into the air pressure sleeve 175, so that the air pressure sleeve 175 is separated from the lowermost bottle cap, the bottle cap falls into the falling chamber 170, and thus, during the reciprocating process, a plurality of filling bottles can be sealed simultaneously, so that the operation is very quick;

the fourth step; when the angle motor 7 continues to rotate, the rotary circular truncated cone 141 rotates by a certain angle, and at the moment, the sealed filling bottles move to the blanking station 164, the filling bottles fall from the blanking station 164 and slide down from the spiral centering block 183, when the bottoms of the filling bottles fall to the top of the vertical feather layers 185, the driving motor 17 drives the annular wheel 181 to rotate through the conveying belt 13, the annular wheel 181 enables the vertical feather layers 185 to rotate, the vertical feather layers 185 drive the filling bottles to stand under the action of the spiral centering block 183, and along with the rotation of the vertical feather layers 185, when the filling bottles are in contact with the limiting block 19, the limiting block 19 can drive the filling bottles to the blanking sleeve 187 and fall into the recovery box 20 from the inside of the blanking cloth sleeve 188, so that the filling bottles can be ensured to be in a vertical state all the time, and the conveying stability of the filling bottles is ensured.

As used in the specification and in the claims, certain terms are used to refer to particular components. As one skilled in the art will appreciate, manufacturers may refer to a component by different names. This specification and claims do not intend to distinguish between components that differ in name but not function. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. "substantially" means within an acceptable error range, and a person skilled in the art can solve the technical problem within a certain error range to achieve the technical effect basically.

It is noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a good or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such good or system. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of additional like elements in the article or system in which the element is included.

The foregoing description shows and describes several preferred embodiments of the invention, but as before, it is to be understood that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. A biological immune cell culture fluid extraction system, comprising: the device comprises a base (1), a culture solution storage box (2) is fixedly connected to the middle of the top of the base (1), a pressurization air pump (3) is fixedly connected to the right side of the top of the culture solution storage box (2), a pressure balancing device (4) is fixedly connected to the right side of the culture solution storage box (2), a pressurization extraction mechanism (12) is fixedly communicated to the top of the culture solution storage box (2), a driving motor (17) is fixedly connected to the right side of the top of the base (1), a driving wheel is fixedly connected to the output end of the driving motor (17), a one-way air outlet valve (6) is sleeved below the inner cavity of the pressurization extraction mechanism (12) and at the connection position with the culture solution storage box (2), a one-way air inlet valve is sleeved at the front side of the inner wall of the pressurization extraction mechanism (12), and two angle motors (7) are fixedly connected to the side surface below the pressurization extraction mechanism (12), the output fixedly connected with tooth axle (8) of angle motor (7), the pressure boost is drawed mechanism (12) middle part cover and is equipped with and changes cover (10), it is equipped with fixed cover (11) to change cover (10) to go up the cover, annular tooth's socket (9) have been seted up to fixed cover (11) side surface below, tooth axle (8) meshing is in annular tooth's socket (9), the pressure boost is drawed mechanism (12) inner chamber middle part intercommunication and is had two feed liquor pipes (5), the bottom of feed liquor pipe (5) runs through the top of culture solution storage box (2) and extends to the below of inner chamber, the pressure boost is drawed mechanism (12) and is located the top cover of fixed cover (11) is equipped with reinforced slewing mechanism (14), the bottom fixed connection of reinforced slewing mechanism (14) is in the top fixed connection of fixed cover (11), base (1) top fixed connection has fixed support cover (15), fixed support cover (15) top fixedly connected with bottling feeding mechanism (16), the cover is equipped with recovery unit (18) on fixed support cover (15), the drive wheel pass through conveyer (13) with recovery unit (18) transmission is connected, both sides all are provided with limit baffle (19) around bottling feeding mechanism (16), just be located on base (1) recovery unit (18) below is provided with collection box (20).

2. The system for extracting culture solution of biological immune cells as claimed in claim 1, wherein: the bottle filling and supplying mechanism (16) comprises an annular rail (161), an annular blanking mechanism (162) is fixedly connected to the annular rail (161), the top of the annular blanking mechanism (162) is communicated with a plurality of bottle blanking groove stations (163), the top of the annular rail (161) is located at the bottle blanking groove stations (163) and is provided with a bottle stopper supplying station (166), the top of the inner wall of the annular rail (161) is arranged at a distance from the bottle blanking groove stations (163) and the bottle stopper supplying station (166) and is provided with a blanking station (164), two guide blocks (165) are arranged between the bottle blanking groove stations (163) and the bottle stopper supplying station (166), the bottom of the inner cavity of the bottle stopper supplying station (166) is provided with an inclined cavity communicated with a falling cavity (170), and an annular sleeve (167) is sleeved below the inner cavity of the bottle stopper supplying station (166), an annular air bag groove (168) is formed in the inner wall of the annular sleeve (167), an air bag sleeve (169) is sleeved in the annular air bag groove (168), an installation groove (171) is formed in the top of the inner wall of the falling cavity (170), an electric push rod (174) is fixedly connected to the top of the inner wall of the installation groove (171), a small motor (173) is fixedly connected to the free end of the electric push rod (174), a cover sleeve (172) is fixedly connected to the output end of the small motor (173), an air pressure sleeve (175) penetrates through the installation groove (171) and the bottle stopper supply station (166), one side of the air pressure sleeve (175) is communicated with the annular air bag groove (168) through an air flow pipe (176), a sealing plug (177) is sleeved on the right side of the inner cavity of the air flow pipe (176), a connecting rod is hinged to the right side of the sealing plug (177), and a movable groove (178) is formed in one side of the inner wall of the installation groove (171), the movable groove (178) is sleeved with an extrusion block (179), and the extrusion block (179) is hinged with the connecting rod.

3. The system for extracting culture solution of biological immune cells as claimed in claim 2, wherein: the pressurizing extraction mechanism (12) comprises a pressure sleeve (121), a pressurizing sleeve (122) is sleeved at the bottom of the pressure sleeve (121), a hydraulic telescopic cylinder (123) is fixedly connected to the top of the pressure sleeve (121), a push rod (125) is fixedly connected to the output end of the hydraulic telescopic cylinder (123), the bottom end of the push rod (125) extends to a pressurizing plate (1251) which is fixedly connected to the inside of the pressurizing sleeve (122), a fixing plate (124) is sleeved on the push rod (125), a plurality of transverse grooves (127) are formed in the inner cavity side wall of the fixing plate (124) at equal intervals, conveying wheels (128) are movably connected to the left side and the right side of the inner cavity of each transverse groove (127), conveying chains are sleeved on the two conveying wheels (128), a lifting gear (129) is fixedly connected to one side of each conveying wheel (128), and an outer gear sleeve (126) is sleeved on the push rod (125), outer tooth cover (126) and inboard promotion gear (129) meshing, fixed plate (124) bottom is the annular and runs through and is provided with promotion rack (131), promotion rack (131) and outside promotion gear (129) meshing, pressure cover (121) middle part cover is equipped with the baffle, equidistant suction cover (130) that runs through on the baffle, suction cover (130) endotheca is equipped with suction stopper (132), the top of suction stopper (132) with promote rack (131) fixed connection, the inner chamber intercommunication of suction cover (130) has extension pipe (134), a plurality of bracing pieces (135) have been seted up to the distance that pressure cover (121) side surface is individual, bracing piece (135) endotheca is equipped with communicating pipe (136), communicating pipe (136) with suction cover (130) inner chamber intercommunication, the inlet end of communicating pipe (136) is provided with one-way water valve (133), the outer end of the supporting rod (135) is fixedly connected with a discharge valve (137), the discharge valve (137) is communicated with the communicating pipe (136), the bottom end of the discharge valve (137) is communicated with a filling head (138), and two liquid level meters (139) are arranged at the bottom of the discharge valve (137).

4. The system for extracting culture solution of biological immune cells as claimed in claim 3, wherein: the feeding rotating mechanism (14) comprises a rotating circular table (141), bottle clamping grooves (1401) with the same number as bottle body blanking groove stations (163) are formed in the side surface of the rotating circular table (141), two inner extending grooves are formed in the side surface of the rotating circular table (141), guide blocks (165) are located in the inner extending grooves, a square groove (142) is formed in one side of the inner wall of the bottle clamping groove (1401), two sliding rods (143) are fixedly connected in the square groove (142), movable blocks (144) are sleeved on the two sliding rods (143), one side of each movable block (144) is elastically connected to one side of the inner wall of the square groove (142) through an extrusion spring (145), an extrusion rod (150) is fixedly connected to one side of each movable block (144), a hinge block (151) is fixedly connected to the outer end of the corresponding pressing rod (150), and an arc-shaped block (152) is fixedly connected to one side of each hinge block (151), the utility model discloses a plastic extruding machine, including arc piece (152), clamping plate (153), sponge board (154), outer wall fixedly connected with axle bed (155) of clamping plate (153), swivel (156) are connected to the internal rotation of axle bed (155), one side fixedly connected with arc pole (157) of swivel (156), one side fixedly connected with elastic sleeve (146) of extrusion pole (150), be provided with elastic groove (147) in elastic sleeve (146), elastic groove (147) have sliding block (149) through reset spring (148) elastic connection, sliding block (149) with arc pole (157) are articulated.

5. The system for extracting culture solution of biological immune cells as claimed in claim 4, wherein: the recovery device (18) comprises an annular wheel (181), the annular wheel (181) is sleeved on the fixed support sleeve (15), a conveying belt (13) is sleeved on the annular wheel (181), the top of the annular wheel (181) is fixedly connected with a vertical woolen layer (185), the fixed support sleeve (15) is sleeved with an annular conveying sleeve (182), the inner wall of the annular conveying sleeve (182) is fixedly connected with spiral centering blocks (183) at equal intervals, the annular conveying sleeve (182) is fixedly connected with an outer cover (184) through a limit baffle (19), the left side and the right side of the outer cover (184) are both provided with a discharge groove (186), a blanking sleeve (187) is sleeved in the discharge groove (186), the bottom of the inner wall of the blanking sleeve (187) is fixedly connected with a blanking cloth sleeve (188), arc-shaped limiting plates (189) are fixedly connected to the two sides of the blanking cloth cover (188).

6. The system for extracting culture solution of biological immune cells as claimed in claim 5, wherein: one-way air outlet valve (6) is including square frame, square frame equidistance elastic connection has the pivot, the cover is equipped with the baffle in the pivot, and two adjacent baffles are the contact of V-arrangement.

7. The extraction method of the biological immune cell culture fluid extraction system according to the claims 1-6, characterized in that: the method comprises the following steps:

firstly, when the rotary circular table (141) rotates for a certain angle, filling bottles in each bottle body blanking groove station (163) fall into a bottle clamping groove (1401), the filling bottles are positioned between two clamping plates (153) and an arc-shaped block (152) and are in contact with a sponge plate (154), an angle motor (7) continues to rotate for a communicated angle, the surface of the filling bottles between the two clamping plates (153) and the arc-shaped block (152) is in contact with the outer wall of a guide block (165), the arc-shaped bulge part of the guide block (165) extrudes the filling bottles more and more obviously in the process of rotating the circular table (141), the filling bottles can extrude the two clamping plates (153) and the arc-shaped block (152) towards the axis of the rotary circular table (141), so that an extrusion rod (150) moves towards the inside of a square groove (142), and simultaneously, a movable block (144) moves on two sliding rods (143) and extrudes an extrusion spring (145), so that the filling bottle moves towards the inner side of the inner wall of the bottle clamping groove (1401), at the moment, the filling bottle moves to the position for filling liquid, through the use of the mutual matching of the two clamping plates (153) and the arc-shaped block (152), when the filling bottle falls between the two clamping plates (153) and the arc-shaped block (152) and the rotating round platform (141) rotates, the filling bottle can contact the sponge plate (154) by utilizing the action of the guide block (165), and the two clamping plates (153) open towards two sides, because the two clamping plates (153) can elastically rotate, after the filling bottle is completely contacted with the sponge plate (154), under the elastic action of the reset spring (148), the two clamping plates (153) and the arc-shaped block (152) can wrap the filling bottle, so that the moving stability of the filling bottle can be increased, and when the filling bottle passes through the guide block (165) every time, the filling bottle is always positioned at the filling point, so that the stability of the liquid in the tank is ensured, and the problem of liquid overflow is avoided;

secondly, when the filling bottle moves to a liquid filling position, the hydraulic telescopic cylinder (123) drives the push rod (125) to move downwards, the pressurizing plate (1251) below the push rod (125) moves downwards in the pressurizing sleeve (122), gas in the pressurizing sleeve (122) is injected into the culture solution storage box (2) through the pressurizing plate (1251), at the moment, the air pressure inside the culture solution storage box (2) is increased, the liquid level inside the culture solution storage box (2) is raised, culture solution inside the culture solution storage box (2) is injected into the pressure sleeve (121) from the liquid inlet pipe (5), the liquid level of the culture solution in the pressure sleeve (121) is raised, the extension pipe (134) is located below the liquid level of the culture solution, and when the duration inside the culture solution storage box (2) is reduced, the pressure balancing device (4) increases pressure inside the culture solution storage box (2), so that the liquid level can quickly enter the liquid inlet pipe (5) from the moment when the pressurizing plate (1251) moves downwards, and the pressure of the liquid level can be ensured to be quickly increased from the liquid inlet pipe (5) on time In the pressure sleeve (121), the sensitivity of liquid level rise is increased, in the process, an external gear sleeve (126) on the push rod (125) drives the transmission wheel (128) to rotate, a transmission chain on the transmission wheel (128) can enable a lifting gear (129) of the transverse groove (127) to rotate, this causes the lifting gear (129) at the outer side to drive the lifting rack (131) to move upwards, the suction plug (132) at the bottom end of the lifting rack (131) moves upwards in the suction sleeve (130), at this time, the suction sleeve (130) can suck the culture solution through the extension pipe (134), when the culture solution in the pumping sleeve (130) rises, the culture solution enters the communicating pipe (136) from the one-way water valve (133) and is filled in the filling bottle from the discharging valve (137), when the liquid in the filling bottle reaches the liquid level requirement, the liquid level meter (139) can detect the liquid level height and control the one-way water valve (133) to be closed;

a third step; when blanking, a bottle body blanking groove station (163) and a bottle stopper supply station (166) are respectively stacked and placed with a filling bottle and a bottle cap for the filling bottle in sequence, when an angle motor (7) rotates for a fixed angle, when the filling bottle is filled with culture solution, filling products move to the whole lower part of the bottle stopper supply station (166), after the top of a rotating circular truncated cone (140) is separated from the bottom of a falling cavity (170) in an overlapping way, the filling bottle lower than a bottle clamping groove (1401) moves to the position right below the falling cavity (170), at this time, the bottle cap falls on the filling bottle in the bottle clamping groove (1401) from the falling cavity (170), at the moment, the free end of an electric push rod (174) is started to drive a cover sleeve (172) to move downwards, the cover sleeve (172) and the bottle cap on the filling bottle are contacted in the downward process of the cover sleeve (172), the bottle cap is stably sleeved on a bottle mouth, and at the moment, the output end of a small-sized motor (173) rotates, in the process, when the cover sleeve (172) moves downwards, the cover sleeve (172) is contacted with the extrusion block (179), the extrusion block (179) moves towards the movable groove (178), the extrusion block (179) drives the connecting rod to extrude the sealing plug (177), the sealing plug (177) can drive the air pressure in the air pressure sleeve (175) to enter the air bag groove (168) from the air flow pipe (176), the air in the air bag groove (168) is increased, the air bag sleeve (169) in the air bag groove (168) is expanded by the air pressure, the bottle cap positioned in the air bag groove (168) is pressed by the air bag sleeve (169), so that the air bag sleeve (169) clamps the bottle cap, the bottle cap at the lowest part is limited in the process of sealing the bottle cap, and when the cover sleeve (172) moves to the highest part, after the cover sleeve (172) is separated from the extrusion block (179), the air bag sleeve (169) is recovered into the air inlet pressure sleeve (175), so that the air pressure sleeve (175) is separated from and clamped with the bottle cap at the lowest part, and the bottle cap falls into the falling cavity (170), and in the reciprocating process, a plurality of filling bottles can be simultaneously sealed, and the operation is very quick;

the fourth step; when the angle motor (7) continues to rotate, the rotary circular truncated cone (141) rotates for a certain angle, and at the moment, the sealed filling bottles move to the blanking station (164), the filling bottles fall from the blanking station (164) and slide down from the spiral righting block (183), when the bottoms of the filling bottles fall to the top of the vertical feather layer (185), the driving motor (17) drives the annular wheel (181) to rotate through the conveying belt (13), the annular wheel (181) enables the vertical feather layer (185) to rotate, the vertical feather layer (185) drives the filling bottles to stand up under the action of the spiral righting block (183), along with the rotation of the vertical feather layer (185), when the filling bottles are in contact with the limiting block (19), the limiting block (19) can drive the filling bottles to the blanking sleeve (187) and fall into the recovery box (20) from the inside of the blanking cloth sleeve (188), and thus the filling bottles can be ensured to be in a vertical state all the time, and then guaranteed the stability of filling bottle conveying.