CN113133468B - Bactericide for controlling pathogenic bacteria fusarium solani after picking of tsaoko amomum fruits - Google Patents
Bactericide for controlling pathogenic bacteria fusarium solani after picking of tsaoko amomum fruits Download PDFInfo
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- CN113133468B CN113133468B CN202110371549.1A CN202110371549A CN113133468B CN 113133468 B CN113133468 B CN 113133468B CN 202110371549 A CN202110371549 A CN 202110371549A CN 113133468 B CN113133468 B CN 113133468B
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- bactericide
- fusarium solani
- pathogenic bacteria
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 28
- 244000052616 bacterial pathogen Species 0.000 title claims abstract description 27
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 27
- 241000427940 Fusarium solani Species 0.000 title claims abstract description 24
- 235000013399 edible fruits Nutrition 0.000 title abstract description 15
- 241001127714 Amomum Species 0.000 title abstract description 8
- 239000005820 Prochloraz Substances 0.000 claims description 10
- 239000000417 fungicide Substances 0.000 claims description 10
- TVLSRXXIMLFWEO-UHFFFAOYSA-N prochloraz Chemical compound C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl TVLSRXXIMLFWEO-UHFFFAOYSA-N 0.000 claims description 10
- 239000006013 carbendazim Substances 0.000 claims description 6
- 239000005795 Imazalil Substances 0.000 claims description 5
- 229960002125 enilconazole Drugs 0.000 claims description 5
- 230000000855 fungicidal effect Effects 0.000 claims description 5
- 244000222991 cardamomo Species 0.000 claims 1
- 235000005300 cardamomo Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 10
- 241000220223 Fragaria Species 0.000 description 7
- 230000002401 inhibitory effect Effects 0.000 description 7
- 235000021012 strawberries Nutrition 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000005730 Azoxystrobin Substances 0.000 description 4
- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 description 3
- PZBPKYOVPCNPJY-UHFFFAOYSA-N 1-[2-(allyloxy)-2-(2,4-dichlorophenyl)ethyl]imidazole Chemical compound ClC1=CC(Cl)=CC=C1C(OCC=C)CN1C=NC=C1 PZBPKYOVPCNPJY-UHFFFAOYSA-N 0.000 description 3
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 3
- 239000005760 Difenoconazole Substances 0.000 description 3
- 239000005869 Pyraclostrobin Substances 0.000 description 3
- 239000005839 Tebuconazole Substances 0.000 description 3
- WFDXOXNFNRHQEC-GHRIWEEISA-N azoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1OC1=CC(OC=2C(=CC=CC=2)C#N)=NC=N1 WFDXOXNFNRHQEC-GHRIWEEISA-N 0.000 description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 3
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 description 3
- HZRSNVGNWUDEFX-UHFFFAOYSA-N pyraclostrobin Chemical compound COC(=O)N(OC)C1=CC=CC=C1COC1=NN(C=2C=CC(Cl)=CC=2)C=C1 HZRSNVGNWUDEFX-UHFFFAOYSA-N 0.000 description 3
- 241001346334 Amomum tsao-ko Species 0.000 description 2
- 239000005843 Thiram Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- -1 enoyl manganese zinc Chemical compound 0.000 description 2
- 230000002070 germicidal effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 229960002447 thiram Drugs 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- XERJKGMBORTKEO-VZUCSPMQSA-N (1e)-2-(ethylcarbamoylamino)-n-methoxy-2-oxoethanimidoyl cyanide Chemical compound CCNC(=O)NC(=O)C(\C#N)=N\OC XERJKGMBORTKEO-VZUCSPMQSA-N 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 239000005747 Chlorothalonil Substances 0.000 description 1
- 239000005756 Cymoxanil Substances 0.000 description 1
- 239000005867 Iprodione Substances 0.000 description 1
- 239000005802 Mancozeb Substances 0.000 description 1
- 229920001090 Polyaminopropyl biguanide Polymers 0.000 description 1
- 241000234299 Zingiberaceae Species 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- ONUFESLQCSAYKA-UHFFFAOYSA-N iprodione Chemical compound O=C1N(C(=O)NC(C)C)CC(=O)N1C1=CC(Cl)=CC(Cl)=C1 ONUFESLQCSAYKA-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- KUAZQDVKQLNFPE-UHFFFAOYSA-N thiram Chemical compound CN(C)C(=S)SSC(=S)N(C)C KUAZQDVKQLNFPE-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Dentistry (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- General Chemical & Material Sciences (AREA)
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Abstract
The invention discloses a bactericide for controlling pathogenic bacteria Fusarium solani after picking of tsaoko amomum fruits, which contains any one or more of the following effective components: the bactericide disclosed by the invention can effectively inhibit the growth of pathogenic bacteria fusarium solani hyphae, has a definite and obvious effect, is favorable for fresh-keeping storage of tsaoko fruits, and reduces economic loss caused by rotten and deteriorated fruits due to fusarium solani.
Description
Technical Field
The invention belongs to the technical field of postharvest preservation of fruits, and relates to a bactericide for controlling postharvest pathogenic bacteria of amomum tsao-ko.
Background
Amomum tsaoko Crevost et Lemaire is a perennial evergreen clustered herb of the genus Amomum in the family Zingiberaceae. The fructus Tsaoko can be used for cooking and can also be used as medicinal materials. As the medical value and the economic value of the tsaoko are gradually known, the market demand is increased, and the planting area and the harvest amount are also increased. However, the mature period of the fresh tsaoko fruits is concentrated, the fruits are mature within 10-12 months, and a large amount of tsaoko fruits are infected by the pathogenic bacterium Fusarium solani in the storage process after being picked, so that the tsaoko fruits are rotten and deteriorated, and serious economic loss is caused. Therefore, the method has to actively and effectively control pathogenic bacteria Fusarium solani, and a bactericide with an inhibiting effect on the pathogenic bacteria Fusarium solani after the strawberries are picked is determined, so that an important reference basis is provided for the storage and management of the strawberries.
Disclosure of Invention
The invention aims to provide the bactericide for controlling pathogenic bacteria of fusarium solani after the strawberries are picked, and the bactericide has a definite and obvious bactericidal effect.
Through research, the invention provides the following technical scheme:
the chemical bactericide for controlling the pathogenic bacterium Fusarium solani after the strawberries are picked comprises any one or more of the following effective components: prochloraz, imazalil, difenoconazole, pyraclostrobin, tebuconazole and azoxystrobin.
Furthermore, the bactericide for controlling the pathogenic bacteria fusarium solani after the strawberries are picked contains the effective component prochloraz.
Furthermore, the bactericide for controlling the pathogenic bacteria fusarium solani after the strawberries are a prochloraz-imazalil bactericide, a prochloraz-carbendazim bactericide or a prochloraz bactericide.
The invention has the beneficial effects that: the bactericide for controlling the pathogenic bacteria Fusarium solani after the tsaoko amomum fruits are picked provided by the invention can effectively inhibit the growth of Fusarium solani hyphae, has a definite and obvious effect, is beneficial to fresh-keeping storage of the tsaoko amomum fruits, and reduces economic loss caused by rotten and deteriorated fruits caused by the Fusarium solani.
Drawings
FIG. 1 shows the inhibitory effect of a single chemical fungicide on pathogenic bacteria. Bars and vertical bars represent mean and standard deviation, respectively, of biological replicates, with different letters indicating significant differences (P < 0.05).
FIG. 2 is a graph showing the effect of a single chemical germicide on the colony diameter of pathogenic bacteria.
FIG. 3 shows the inhibitory effect of commercial fungicides on pathogenic bacteria. Bars and vertical bars represent mean and standard deviation, respectively, of biological replicates, with different letters indicating significant differences (P < 0.05).
FIG. 4 is a graph showing the effect of commercial fungicides on pathogen colony diameter.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, preferred embodiments of the present invention will be described in detail below.
Experimental materials and methods
1. Inhibiting effect of single chemical bactericide on pathogenic bacteria
The chemical bactericide single products shown in Table 1 were added to the melt-sterilized PDA medium to prepare a drug-containing medium with a final concentration of 5ppm, 20mL per plate, and an equal amount of sterile water was used as a control. After the medium had solidified, a Fusarium solani cake of 5mm diameter, cultured on normal PDA medium for 7 days, was inoculated into the above plate, sealed with a sealing film, and cultured in an incubator at 28 ℃ for 5 days, and the colony diameter of each plate was measured by the cross method.
TABLE 1 chemical germicide single product
2. Inhibitory Effect of commercial fungicides on pathogenic bacteria
The commercial bactericides shown in table 2 were added to the PDA medium subjected to melt sterilization to prepare drug-containing media having final concentrations shown in table 2 (the in vitro bacteriostatic use concentration is the highest recommended use concentration of the commercial bactericides), each plate was 20mL, and an equal amount of sterile water was used as a control. After the medium had solidified, a Fusarium solani cake of 5mm diameter, cultured on normal PDA medium for 7 days, was inoculated into the above plate, sealed with a sealing film, and cultured in an incubator at 28 ℃ for 5 days, and the colony diameter of each plate was measured by the cross method.
TABLE 2 commercial fungicides
Second, experimental results
1. Inhibiting effect of single chemical bactericide on pathogenic bacteria
In order to effectively prevent and control the rot caused by the pathogenic bacteria Fusarium solani of the tsaoko amomum fruits, a single product of 10 chemical bactericides is selected, and the inhibition effect of the single product on the growth of the hypha of the pathogenic bacteria Fusarium solani is measured. As shown in fig. 1 and 2, 6 chemical bactericides (prochloraz No. 10, imazalil No. 4, difenoconazole No. 2, pyraclostrobin No. 7, tebuconazole No. 3 and azoxystrobin No. 1) can obviously inhibit the growth of pathogenic bacteria hyphae, wherein the prochloraz has the best inhibition effect on the growth of the pathogenic bacteria hyphae, and then the imazalil, the difenoconazole, the pyraclostrobin, the tebuconazole and the azoxystrobin are sequentially added; in addition, 4 chemical bactericides (No. 5 PAPB, No. 6 iprodione, No. 8 PeeG, No. 9 PC8) could not inhibit the growth of the hypha of the pathogenic bacterium Fusarium solani of the tsaoko amomum fruit.
2. Inhibitory Effect of commercial fungicides on pathogenic bacteria
As shown in FIGS. 3 and 4, 13 selected commercial bactericides can inhibit the growth of pathogenic fusarium solani hyphae, but the inhibition effects are greatly different, wherein the inhibition effects of the imidazole-imazazole (B), the imidazole-imazazole (C), the methionine-thiram (E), the imidazole-carbendazim (H), the prochloraz (A) and the carbendazim (L) on the growth of the fusarium solani hyphae are the best, and the hypha growth can be completely inhibited after 5 days of inoculation; the inhibition effects of chitosan (M), enoyl manganese zinc (K), enoyl azoxystrobin (D) and chlorothalonil (J) are inferior, and the diameter of hypha can be reduced by about 40-55 mm; the inhibition effects of oxatone cymoxanil (G), carbendazim thiram (i), carbendazim + mancozeb (F) are relatively weak. Therefore, the commercial bactericide containing the prochloraz component can effectively inhibit the growth of pathogenic bacteria Fusarium solani after the strawberries are picked.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (2)
1. Application of a bactericide containing prochloraz in preparation of a bactericide for controlling pathogenic bacteria Fusarium solani after picking of tsaoko cardamom.
2. The use as claimed in claim 1, wherein the prochloraz-containing fungicide is a prochloraz-imazalil fungicide, a prochloraz-carbendazim fungicide or a prochloraz fungicide.
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| CN202110371549.1A CN113133468B (en) | 2021-04-07 | 2021-04-07 | Bactericide for controlling pathogenic bacteria fusarium solani after picking of tsaoko amomum fruits |
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| CN202110371549.1A CN113133468B (en) | 2021-04-07 | 2021-04-07 | Bactericide for controlling pathogenic bacteria fusarium solani after picking of tsaoko amomum fruits |
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