CN113117006A

CN113117006A - Application of traditional Chinese medicine composition in preparation of anti-skin-aging drugs or cosmetics

Info

Publication number: CN113117006A
Application number: CN202110612844.1A
Authority: CN
Inventors: 张贵民; 张微; 韩振明
Original assignee: Lunan Pharmaceutical Group Corp
Current assignee: Lunan Pharmaceutical Group Corp
Priority date: 2021-03-20
Filing date: 2021-06-02
Publication date: 2021-07-16

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly discloses application of a traditional Chinese medicine composition in an anti-skin-aging medicine or a cosmetic. The traditional Chinese medicine composition is prepared from eight raw medicinal materials such as polygonum multiflorum, aloe, cassia seed, ginseng, medlar, donkey-hide gelatin, immature bitter orange, bighead atractylodes rhizome and the like, and has the effects of nourishing yin, tonifying qi, discharging turbidity and relaxing bowels. The traditional Chinese medicine composition is an effective prescription for resisting skin aging, can effectively inhibit free radical metabolism, inhibit the expression of superoxide dismutase (SOD activity) in the skin, and reduce the contents of Malondialdehyde (MDA) and skin strong proline (Hyp) which are decomposition products of lipid peroxide, thereby effectively relieving the skin aging and playing the effects of maintaining beauty and keeping young.

Description

Application of traditional Chinese medicine composition in preparation of anti-skin-aging drugs or cosmetics

Technical Field

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to an application of a traditional Chinese medicine composition in preparation of an anti-skin-aging medicine or a cosmetic.

Background

With the development and progress of society, people desire to enjoy higher spiritual life, and the requirement for delaying skin aging is more and more urgent. Skin aging is the result of the combined action of various factors, and is mainly divided into two types: one is endogenous aging, also called natural aging, which is mainly affected by genetic factors and is a process of natural progressive aging; the other is exogenous aging caused by the influence of external environmental factors such as ultraviolet radiation, smoking, gravitation, chemicals and the like. Although the specific mechanism of skin aging is not completely understood, people have been deeply aware of the aging mechanism in recent years, and the traditional Chinese medicine aging theory and the modern aging theory are successively established, and the skin aging mechanism is researched from different angles. Currently, theories regarding skin aging include cellular aging and structural function changes, gene regulation, free radical damage, glycosylation, immune disorders, hormonal disorders, and the like.

The theory of skin aging is believed by western medicine to be a free radical theory. Free radicals are intermediate products of normal metabolism, have strong reaction capability, can oxidize various substances in cells to damage biological membranes, can also cross-link macromolecules such as proteins and nucleic acids to influence the normal functions of the macromolecules, and simultaneously can decline the structure, the function, the adaptability and the resistance of the skin. In vivo and in vitro experiments have shown that the deleterious effects of free radicals produced during normal cellular metabolism cause degenerative changes during aging. The aging process of organisms is the accumulation result of free radicals continuously generated by organism histiocytes, and western medicine generally delays skin aging by anti-free radical medicines such as vitamin C and the like.

The theory of the traditional Chinese medicine on skin aging such as the theory of aging caused by spleen deficiency, the theory of aging caused by kidney deficiency, the theory of aging caused by yin-yang imbalance and the like is provided. The theory of spleen deficiency is that the spleen governs transportation and transformation, governs blood, and promotes the transportation and healthy and vigorous of spleen, so that food essence can be distributed to nourish the viscera, six bowels, limbs and bones. Otherwise, the food essence is distributed badly, resulting in deficiency of qi and blood, malnutrition of skin, and lusterless. The theory of deficiency due to kidney deficiency considers that the life process is the process of the abundance or insufficiency of the kidney essence, and the kidney essence is exuberant and excessive to grow teeth; the muscles and bones are strong with the average kidney qi, the body is strong, and the growth is mature; the aging gradually progresses along with the deficiency of kidney qi.

Because of the endless and inexhaustible treatment modes for resisting skin aging, many people have certain doubt and negation about the treatment modes, and the biggest characteristic of traditional Chinese medicine treatment is safety and effectiveness, the pig skin soup and other formulas mentioned earlier in the treatise on typhoid fever can still be used for skin care till now, and the history of delaying skin aging of traditional Chinese medicine is long. At present, the traditional Chinese medicine has made great progress in the aspects of resisting skin aging and beautifying, has received the attention at home and abroad, and has wide prospect. Although the traditional Chinese medicine has slow effect and long treatment course in resisting skin aging and beautifying, the traditional Chinese medicine has the advantages of achieving the effects of beautifying and resisting skin aging by removing velcade of internal organs, having small toxic and side effects, integrally adjusting and achieving the purpose of internal and external treatment.

The formula of the aloe preparation comprises medlar, donkey-hide gelatin, tuber fleeceflower root, ginseng, largehead atractylodes rhizome, immature bitter orange, aloe and cassia seed, and has the main effects of tonifying qi and nourishing yin, and purging turbidity and relaxing bowels, and is used for treating functional constipation with corresponding symptoms. The syndrome differentiation of the traditional Chinese medicine belongs to the syndrome of deficiency of both qi and yin and internal accumulation of toxic factors, and the symptoms comprise constipation, abdominal distension, dry mouth and throat, mental fatigue and hypodynamia, dysphoria with smothery sensation in the chest, red, tender or pale tongue, white or white and greasy tongue coating, deep, thin or smooth and rapid pulse. The previous research is mainly used for treating constipation, and is not used in the field of skin aging resistance. The aloe formulation approval reference is: national drug standard Z20150041; CN201811404355.1 discloses a preparation process of a aloe preparation.

The traditional Chinese medicine considers that visceral sinking is the main reason for external skin aging and pathological changes, the visceral functions of the human body are normal through regulating the visceral organs of the human body, and the human body can achieve the skin-nourishing effect of light and freshness, so that the traditional Chinese medicine has the theory of treating external diseases and internal diseases. This statement is also widely accepted by modern medicine. The beauty-maintaining and toxin-expelling are inseparable, the aloe is organically combined with the defaecation by four methods of 'dredging', 'discharging', 'regulating' and 'tonifying' in the traditional Chinese medicine, and the effects of maintaining beauty and resisting skin aging are achieved by defecating.

Disclosure of Invention

The invention aims to provide a new application of a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for resisting skin aging and beautifying. The application of the invention is discovered in the clinical application process of the company medicine, and has larger commercial value after being verified by related pharmacodynamic tests.

The technical scheme of the invention is as follows:

the application of a traditional Chinese medicine composition in preparing a medicine or a cosmetic for resisting skin aging is disclosed, wherein the traditional Chinese medicine composition is prepared from the following components: fleece-flower root, aloe, cassia seed, ginseng, medlar, donkey-hide gelatin, immature bitter orange and bighead atractylodes rhizome.

The Chinese medicinal composition has the functions of inhibiting free radical metabolism, inhibiting the expression of superoxide dismutase (SOD) in skin, and reducing the contents of Malondialdehyde (MDA) and strong proline (Hyp) in skin, which are decomposed products of oxidized lipid.

Application of the traditional Chinese medicine composition in preparing anti-skin-aging medicines or cosmetics for perimenopausal women.

The traditional Chinese medicine composition is prepared from the following components in parts by weight:

preferably, the traditional Chinese medicine composition is prepared from the following components in parts by weight:

further preferably, the traditional Chinese medicine composition is prepared from the following components in parts by weight:

the traditional Chinese medicine composition is prepared into a clinically acceptable oral preparation or a skin external preparation by adding pharmaceutically acceptable auxiliary materials in a conventional process.

The clinically acceptable oral preparation is capsules, pills, tablets, granules and oral liquid; preferably capsules.

The skin external preparation is skin cream, lotion, gel, cosmetic water or facial mask.

In order to obtain the oral dosage form, the invention does not limit the types of the auxiliary materials, and the auxiliary materials comprise one or more of fillers, binders, disintegrants, surfactants, dispersing agents, diluents, lubricants, preservatives, coloring agents, flavoring agents and the like.

In order to obtain the skin external preparation, the category of the auxiliary materials is not limited, and the auxiliary materials comprise water, glycerol, ethanol, polyethylene glycol, trehalose, disodium EDTA and the like, and the skin external preparation is prepared by adding auxiliary materials or auxiliary active ingredients commonly used in pharmacy or cosmetics, wherein the auxiliary ingredients comprise collagen, sodium hyaluronate or vitamins.

The process for preparing the compositions of the present invention will be further illustrated below.

The preparation method of the traditional Chinese medicine composition comprises the following steps:

A. extracting Ginseng radix, Polygoni Multiflori radix, semen Cassiae and Aloe with ethanol under reflux, filtering, and collecting filtrate.

B. Decocting fructus Lycii and Atractylodis rhizoma in water, and filtering; collecting filtrate, concentrating to obtain fluid extract with relative density of 1.10-1.20(80 deg.C), cooling to room temperature, stirring, adding ethanol to make ethanol content reach 60% (20 deg.C), refrigerating, standing for more than 24 hr, and filtering.

C. Mixing the decoction and ethanol precipitation filtrate with above ethanol extractive solution, concentrating to obtain soft extract with relative density of 1.20-1.25(70 deg.C), vacuum belt drying, and pulverizing.

D. The preparation process comprises the following steps: adding the above colla Corii Asini powder into dry powder, directly or indirectly adding pharmaceutically acceptable excipient by conventional process, mixing, and making into oral medicinal preparation or skin external preparation.

In a preferred embodiment, the oral pharmaceutical preparation is a capsule, and the preparation method comprises the following steps:

A. reflux-extracting Ginseng radix, Polygoni Multiflori radix, semen Cassiae and Aloe with 60% ethanol for 2 times, 10 times for the first time and 8 times for the second time, each for 2 hr, filtering, and mixing filtrates.

B. Decocting fructus Lycii and Atractylodis rhizoma in water for 2 times, adding 8 times of water for the first time, soaking for 1 hr, decocting for 1.5 hr, and filtering; adding 6 times of water for the second time, decocting for 1.5 hr, mixing filtrates, concentrating to obtain fluid extract with relative density of 1.10-1.20(80 deg.C), standing at room temperature, stirring, adding ethanol to make ethanol content reach 60% (20 deg.C), refrigerating, standing for more than 24 hr, and filtering.

D. The preparation process comprises the following steps: adding the above colla Corii Asini powder and adjuvant into dry powder, mixing, granulating by dry method, pressing into granule, and making into capsule.

In another preferred embodiment the dosage form is a pellet formulation, and the process for preparing the pellet formulation comprises the steps of:

D. The preparation process comprises the following steps: adding the above colla Corii Asini powder and dextrin into the dry powder, adding adjuvant, making pill, drying, polishing, coating, and making pill.

In another preferred embodiment, the skin external preparation is a gel. The preparation method of the gel comprises the following steps:

D. The preparation process comprises the following steps: adding colla Corii Asini powder, appropriate amount of water and gel matrix into the above powder, and making into gel.

In another embodiment, the formulation is a lotion. The preparation method comprises the following steps:

D. The preparation process comprises the following steps: mixing the above powders with colla Corii Asini powder, appropriate amount of water, glycerol and surfactant, and making into cosmetic lotion.

Compared with the prior art, the invention has the following technical advantages and beneficial effects:

the traditional Chinese medicine composition has an effective formula for resisting skin aging, can effectively inhibit free radical metabolism, inhibit the expression of superoxide dismutase (SOD activity) in skin, and reduce the content of Malondialdehyde (MDA) and skin strong proline (Hyp) which are decomposition products of lipid peroxide, thereby effectively relieving skin aging and playing the effects of maintaining beauty and keeping young.

The inventor develops corresponding animal test research on the effect of the traditional Chinese medicine composition on skin aging. With age, the most intuitive manifestations of aging are tarnish, appearance of wrinkles, pigmentation, etc. Skin aging is the reaction of whole body aging on skin, and the expression levels of superoxide dismutase (SOD activity), Malondialdehyde (MDA) which is the decomposition product of lipid peroxide, and strong proline content (Hyp) in skin determine the aging degree of skin.

It should be noted that the animal experimental study is a sample obtained by the representative formulation and the preparation method of the present invention, and the other formulations and products obtained by the preparation method of the present invention relate to experiments and results thereof, which are not exhaustive herein due to space limitations.

Experimental example 1 Effect of Hlo preparation on moisture and tissue structure of aged skin in mice

1 materials of the experiment

1.1 Experimental animals and feeds

40 SPF-grade Kunming mice, each half of male and female, 6-8 weeks old, 20 +/-4 g of body weight, provided by Jinanpunyue laboratory animal Breeding company Limited, and the license number of the laboratory animal: SYXK (lu) 20200109. The animals are adaptively raised in a clean-grade animal laboratory for 1 week before experiment, the male and female parts are separated, the room temperature is 20-25 ℃, the relative humidity is 40% -60%, natural illumination is carried out, and the animals freely drink water and eat food.

1.2 instruments, reagents and drugs

An electronic analytical balance model AG285 (Mettler-Toledo, Switzerland); an SK-III skin moisture meter (Shenzhen Phiss Keel electronic factory); DM2500 microscope (Leica, germany); d-galactose (analytically pure) (produced by Beijing Bylendi Biotechnology Ltd.); the tested medicine is a Hui Tong capsule (manufactured by Lu nan Mupu pharmacy Co., Ltd., batch No. 20190112), and the tested medicine is prepared into corresponding concentration by normal saline during the experiment; the positive control drug was vitamin E (Touchen times Jian GmbH, lot number 6940863603113).

2 method of experiment

2.1 grouping and Molding

50 healthy Kunming mice are divided into 5 groups at random, each group comprises 10 male and female halves, namely a normal control group, a model control group, a vitamin E positive control group (abbreviated as a "positive control group") (0.1g/kg), a aloe cathartic capsule high dose group (abbreviated as a "test high dose group") (0.105g/kg), and a aloe cathartic capsule low dose group (abbreviated as a "test low dose group") (0.0035 g/kg). Normal control group mice were injected with normal saline subcutaneously, the other groups of mice were injected with D-galactose solution (1g/kg) subcutaneously for 1 time/D for 6 weeks, and subacute skin aging mouse models were established, and injections were performed according to sterile procedures.

2.2 administration of drugs

D-galactose is injected, the animals in each group are intragastrically administered with corresponding drugs, dose reduction is carried out according to dose reduction coefficients of different animals in annexes in the guideline of clinical research on new traditional Chinese medicines, a positive control group is intragastrically administered with vitamin E0.1 g/kg, a test high dose group and a test low dose group are intragastrically administered with first aloe particles 0.105g/kg and 0.0035g/kg respectively, the administration volume is 10ml/kg, and a normal control group and a model control group are intragastrically administered with equal volume of normal saline for 1 time/D for 6 weeks.

2.3 skin moisture determination

The skin moisture content was measured 1 time every 7 days during the experiment using a skin moisture tester and the percent skin moisture was recorded.

2.4 skin tissue structure observation:

animals were sacrificed 1h after the last administration, shoulder and neck skin was clipped and fixed in 4% neutral paraformaldehyde solution, and histological observation of skin morphology was performed after HE staining.

3 results of the experiment

3.1 Effect of Aloe Vera preparations on aging skin moisture

The moisture content of the skin in the first and second weeks of the model is not obviously changed, and the moisture content of the skin in the model group is gradually reduced from the third week. The skin moisture content of the administered group was gradually increased compared to the model group, and by the fifth week, the skin moisture content of the high dose group was significantly increased (P <0.01) and the skin moisture content of the medium dose group was significantly increased (P <0.01) in the test. By week six, the skin moisture content was significantly higher in the experimental high dose group and the experimental low dose group than in the model group (P < 0.05). The aloe preparation is shown to be capable of remarkably improving the reduction of the water content of the aged skin caused by long-term injection of D-galactose. The results are shown in Table 1.

TABLE 1 Effect of aloe on moisture content of aged skin tissue (x + -s, n-10)

Note: comparison with normal control group: p <0.05 is denoted by "Δ"; comparison with model control group: p <0.05 is indicated by ". times..

3.2 Effect of Aloe Vera preparations on the texture of aging skin

The observation result of the skin tissue structure shows that the skin surface cornification layer of the mice of the aging model group is thick, the pigmentation of the basal layer is obvious, the dermis layer is thin, the collagen fiber of the dermis layer is sparse, the hyaloid change exists, and the glandular tube is not changed. After the aloe preparation is given, the skin cuticle layer of a mouse in a low-dose drug group is thicker, the substrate pigmentation is more obvious, the dermis collagen fiber is slightly more, and the sweat gland and the sebaceous gland are not obviously changed; the test shows that the skin surface cornified layer of the high-dose group mouse is thinned, the pigmentation of the basal layer is not obvious, the dermis layer is thickened, the collagen fiber is dense and rich, the glandular duct is expanded, and the secretion is vigorous. It is shown that the aloe preparation can improve the tissue structure of aged skin.

The experimental results show that the aloe preparation can increase the skin moisture content of the aging model mouse and effectively improve the conditions of dry, loose and rough skin, thereby effectively relieving skin aging and playing the effects of maintaining beauty and keeping young.

Experimental example 2 Effect of Hhui preparation on antioxidant ability of skin aging mice

1 materials of the experiment

1.1 Experimental animals and feeds

The same as in experimental example 1.

1.2 instruments, reagents and drugs

An electronic analytical balance model AG285 (Mettler-Toledo, Switzerland); thermo Scientific medidrug low temperature high speed centrifuge (semer fly, usa); spectrophotometer (shimadzu); Scilogex-MX-S vortex mixer (Sailojike, USA); SOD test box, MDA test box, Hyp test box (Nanjing institute of bioengineering); d-galactose (analytically pure) (produced by Beijing Bylendi Biotechnology Ltd.); the tested medicine is a Hui Tong capsule (manufactured by Lu nan Mupu pharmacy Co., Ltd., batch No. 20190112), and the tested medicine is prepared into corresponding concentration by normal saline during the experiment; the positive control drug was vitamin E (Touchen times Jian GmbH, lot number 6940863603113).

2 method of experiment

2.1 grouping, Molding and administration

The same as in experimental example 1.

2.2 treatment of the Material

(1) The animals were sacrificed 1h after the last dose and a suitable amount of skin on the back of the neck was taken. After the blood is flushed by double distilled water, the blood is wiped off, placed in a plastic vial, immediately put into liquid ammonia, and then the plastic vial is taken out and kept in a refrigerator at the temperature of 45 ℃ below zero for standby.

(2) Preparation of tissue homogenate: the skin tissue mass was removed from the freezer and rinsed in chilled saline to remove blood, wiped dry with filter paper, weighed, and placed in a 10ml beaker. Measuring 6 times of cold physiological saline by using a pipette, placing the cold physiological saline into a beaker, quickly shearing tissue blocks by using scissors, pouring the sheared tissue blocks into a glass homogenate tube, flushing the residual tissue blocks in the beaker by using 3 times of cold physiological saline, pouring the cold physiological saline into the homogenate tube together for homogenate for 15min, centrifuging the prepared homogenate for 10min at a low temperature of 3000r/min, and sucking supernatant for later use.

2.3 serum antioxidant index determination

2.3.1 determination of SOD content (xanthine oxidase method)

(1) The determination principle is as follows: the reaction system of xanthine and xanthine oxidase is used to generate superoxide anion free radical, which oxidizes hydroxylamine to form nitrite, which is purple red under the action of color developing agent, and the visible spectrophotometer is used to measure the absorbance at 550 nm. Because superoxide dismutase (SOD) has specific inhibition effect on superoxide anion free radicals, when a measured sample contains SOD, the formation of nitrite is reduced, the absorbance value of a measuring tube during colorimetric is lower than that of a control tube, and the SOD activity in the measured sample can be obtained by the technology.

(2) The method comprises the following operation steps: adding corresponding reagent according to the operation method specified in the SOD test box specification, mixing well with vortex mixer, and placing in 37 deg.C constant temperature water bath for 40 min. Adding 2ml of color developing agent, mixing uniformly, pouring into a colorimetric tube after 10min, and measuring the absorbance value at 550nm by taking distilled water as a reference.

(3) Calculating the formula: SOD activity (u/mgprot) in tissue homogenate (control tube absorbance-determination)/control tube absorbance ÷ 50% multiplied by total volume of reaction solution/sample volume (ml) ÷ protein content in tissue (mgprot/ml)

Note: SOD activity unit definition: the SOD amount corresponding to the SOD inhibition rate of 50% in 1ml of reaction solution per mg of tissue protein is one SOD activity unit.

2.3.2 measurement of Hyp content (sample alkaline hydrolysis)

(1) The determination principle is as follows: the oxidation product of hydroxyproline (Hyp) generated under the action of an oxidizing agent reacts with dimethylaminobenzaldehyde to form mauve, and the content of hydroxyproline (Hyp) can be calculated according to the shade of the color.

(2) The method comprises the following operation steps: pretreating a skin sample before testing according to a hydroxyproline test box in the tissue, adding the sample, uniformly mixing the sample after adding the sample, carrying out water bath at 60 ℃ for 15 minutes, cooling the mixture, centrifuging the mixture for 10 minutes at 3500 rpm, taking supernatant at 550nm, and measuring the absorbance value at 550nm in a colorimetric tube by taking distilled water as reference.

(3) Calculating the formula: hydroxyproline content (ug/mg wet weight) ═ absorbance of measurement tube-absorbance of blank tube)/(absorbance of standard tube-absorbance of blank tube) × standard tube content (5 μ g/ml) × total volume of hydrolysate (10 ml)/tissue humidity (mg)

2.3.3 determination of the MDA content (TBA method)

(1) The determination principle is as follows: the oxygen radicals can oxidize unsaturated fatty acids in cellular lipids to produce various lipid peroxides. The main product of the reaction of Malondialdehyde (MDA) to lipid peroxide, heated under acetic acid conditions, reacts with thiobarbituric acid (TBA) to produce a red compound, which has a maximum absorption peak at 520 nm.

(2) The method comprises the following operation steps: adding corresponding reagents according to the operation method specified by the MDA determination kit specification, uniformly mixing by using a vortex mixer, fastening the opening of a test tube by using a preservative film, pricking a small hole by using a needle, carrying out water bath at 95 ℃ for 40min, taking out, cooling by flowing water, centrifuging at 3500 rpm for 10min, taking supernatant, measuring A532nm, and calculating the MDA content in the tissue.

(3) Calculating the formula: tissue MDA content (nmol/mgprot) ═ MDA content (assay tube absorbance-assay blank tube absorbance)/(standard tube absorbance-standard blank tube absorbance) × standard concentration (10nmol/ml) ÷ protein content (mgprot/ml)

2.4 statistical treatment

The analysis was performed using SPSS19.0 statistical software and the results are expressed as "mean. + -. standard deviation (x. + -.s)". The comparison among groups was performed by one-way anova, with P <0.05 indicating that the difference was statistically significant.

3 results of the experiment

3.1 Effect of Hhui preparation on serum SOD content in mice

Compared with a normal control group, the SOD content in the serum of mice in an aging group model is obviously reduced, the difference is significant (P is less than 0.05), and the success of model building of the aging model is prompted; compared with the model group, the serum SOD content of the mice is obviously increased (P is less than 0.05) after the aloe cathartic capsule with low dose or high dose is administered, and the serum SOD content of the mice in the test low dose group is close to the serum SOD content of the mice in the control group. The results are shown in Table 2.

3.2 Effect of Hui preparation on serum Hyp content in mice

Compared with a normal control group, the content of Hyp in the serum of the model group mouse is obviously reduced (P is less than 0.05), and the success of model building of the aging model is prompted; compared with the model group, the serum Hyp content of the mice is remarkably increased (P <0.05) after the HlI purgative capsule with low dose or high dose is administered, and the serum Hyp content of the mice in the low dose group can be restored to be close to the serum Hyp content of the mice in the control group. The results are shown in Table 2.

3.3 Effect of Aloe Vera preparations on the serum MDA content in mice

Compared with a normal control group, the content of MDA in serum of mice in an aging group model is remarkably increased (P is less than 0.05), and the success of model building of the aging model is prompted; compared with the model group, after the aloe cathartic capsule with low dose or high dose is given, the MDA content in the serum of the mice is obviously reduced (P is less than 0.05), and the MDA content in the serum of the mice of the low dose group can be recovered to be close to the MDA content in the serum of the mice of the control group. The results are shown in Table 2 and FIGS. 1 to 3.

TABLE 2 Effect of the Aloe preparation on the levels of SOD, Hyp and MDA in the sera of mice (x. + -.s, n ═ 10)

The above experimental results show that the aloe preparation can effectively inhibit free radical metabolism, inhibit the expression of superoxide dismutase (SOD activity) in the skin, and reduce the contents of Malondialdehyde (MDA) and skin strong proline (Hyp) which are decomposition products of lipid peroxide, thereby effectively relieving skin aging and playing the effects of maintaining beauty and keeping young.

Drawings

Figure 1 is the effect of the aloe preparation on mouse serum SOD levels (x ± s, n ═ 10); comparison with normal control group: p <0.05 is denoted by "Δ"; comparison with model control group: p <0.05 is denoted by "+";

fig. 2 is the effect of the aloe preparation on the serum Hyp content of mice (x ± s, n ═ 10); comparison with normal control group: p <0.05 is denoted by "Δ"; comparison with model control group: p <0.05 is denoted by "+";

figure 3 is the effect of the aloe preparation on the mouse serum MDA content (x ± s, n ═ 10); comparison with normal control group: p <0.05 is denoted by "Δ"; comparison with model control group: p <0.05 is indicated by ". times..

Detailed Description

The invention is further illustrated by the following specific examples, which are not to be construed as limiting the invention in any way, as will be appreciated by those skilled in the art.

EXAMPLE 1 preparation of granules

A. Extracting Polygoni Multiflori radix, semen Cassiae, Aloe and Ginseng radix with 60% ethanol under reflux for 2 times, 10 times the amount of the ethanol for the first time, 8 times the amount of the ethanol for the second time, 2 hr each time, filtering the medicinal liquid, recovering the combined filtrate, and concentrating.

B. Decocting fructus Lycii, fructus Aurantii Immaturus, and Atractylodis rhizoma in water for 2 times, adding 8 times of water for the first time, soaking for 1 hr, decocting for 1.5 hr, and filtering; adding 6 times of water for the second time, decocting for 1.5 hr, mixing filtrates, concentrating to obtain fluid extract with relative density of 1.10(80 deg.C), cooling to room temperature, stirring, adding ethanol to make ethanol content reach 60% (20 deg.C), refrigerating, standing for more than 24 hr, and filtering.

C. Mixing the decoction and ethanol precipitation filtrate with the above ethanol extractive solution, concentrating to obtain soft extract with relative density of 1.20(70 deg.C), drying, and pulverizing to obtain dry powder.

D. The preparation process comprises the following steps: adding appropriate amount of colla Corii Asini powder and starch into dry powder, mixing, dry granulating, making into granule, and packaging.

EXAMPLE 2 preparation of tablets

A. Extracting Polygoni Multiflori radix, semen Cassiae, Aloe and Ginseng radix with 60% ethanol under reflux for 2 times, 10 times the amount of the ethanol for the first time, 8 times the amount of the ethanol for the second time, 2 hr each time, filtering respectively, and mixing filtrates.

B. Decocting fructus Lycii, fructus Aurantii Immaturus, and Atractylodis rhizoma in water for 2 times, adding 8 times of water for the first time, soaking for 1 hr, decocting for 1.5 hr, and filtering; adding 6 times of water for the second time, decocting for 1.5 hr, mixing filtrates, concentrating to obtain fluid extract with relative density of 1.20(80 deg.C), cooling to room temperature, stirring, adding ethanol to make ethanol content reach 60% (20 deg.C), refrigerating, standing for more than 24 hr, and filtering.

C. Mixing the decoction and ethanol precipitation filtrate with the above ethanol extractive solution, concentrating to obtain soft extract with relative density of 1.25(70 deg.C), drying, and pulverizing to obtain dry powder.

D. The preparation process comprises the following steps: adding the above colla Corii Asini powder and starch into dry powder, mixing, granulating by dry method to obtain granule, and tabletting.

EXAMPLE 3 preparation of capsules

B. Decocting fructus Lycii, fructus Aurantii Immaturus, and Atractylodis rhizoma in water for 2 times, adding 8 times of water for the first time, soaking for 1 hr, decocting for 1.5 hr, and filtering; adding 6 times of water for the second time, decocting for 1.5 hr, mixing filtrates, concentrating to obtain fluid extract with relative density of 1.15(80 deg.C), cooling to room temperature, stirring, adding ethanol to make ethanol content reach 60% (20 deg.C), refrigerating, standing for more than 24 hr, and filtering.

C. Mixing the decoction and ethanol precipitation filtrate with the above ethanol extractive solution, concentrating to obtain soft extract with relative density of 1.23(70 deg.C), drying, and pulverizing to obtain dry powder.

D. The preparation process comprises the following steps: adding the above colla Corii Asini powder and starch into dry powder, mixing, dry granulating to obtain granule, and encapsulating.

EXAMPLE 4 preparation of pellets

D. The preparation process comprises the following steps: adding appropriate amount of colla Corii Asini powder and starch into dry powder, mixing, making pill, drying, polishing, and waxing.

EXAMPLE 5 preparation of capsules

EXAMPLE 6 preparation of gels

D. The preparation process comprises the following steps: adding the above colla Corii Asini powder, water and gel matrix into the dry powder, and mixing to obtain gel.

Example 7 preparation of astringent

EXAMPLE 8 preparation of facial mask

D. Mixing the above dry powder with colla Corii Asini powder, and concocting with egg white or sterile water to obtain paste.

Claims

1. The application of a traditional Chinese medicine composition in preparing a medicine or a cosmetic for resisting skin aging is disclosed, wherein the traditional Chinese medicine composition is prepared from the following components: fleece-flower root, aloe, cassia seed, ginseng, medlar, donkey-hide gelatin, immature bitter orange and bighead atractylodes rhizome.

2. The use as claimed in claim 1, wherein the Chinese medicinal composition has effects in inhibiting free radical metabolism, inhibiting the expression of superoxide dismutase (SOD) in skin, and reducing the content of Malondialdehyde (MDA) which is a decomposition product of oxidized lipid and strong proline (Hyp) in skin.

3. The use of claim 1, wherein the use of the composition is in the preparation of a medicament or cosmetic for anti-skin aging in perimenopausal women.

4. The use of any one of claims 1 to 3, wherein the Chinese medicinal composition is prepared from the following components in parts by weight:

5. the use of claim 4, wherein the Chinese medicinal composition is prepared from the following components in parts by weight:

6. the use of claim 5, wherein the Chinese medicinal composition is prepared from the following components in parts by weight:

7. the use of claim 4, wherein the Chinese medicinal composition is prepared into a clinically acceptable oral preparation or a skin external preparation by adding pharmaceutically acceptable auxiliary materials in a conventional process.

8. The use of claim 7, wherein the clinically acceptable oral formulation is a capsule, a pill, a tablet, a granule, an oral liquid.

9. The use of claim 8, wherein the clinically acceptable oral formulation is a capsule.

10. The use according to claim 8, wherein the skin external preparation is a skin cream, lotion, gel, lotion or pack.