CN113109306A - Sorting device and sorting method with aflatoxin detection function - Google Patents
Sorting device and sorting method with aflatoxin detection function Download PDFInfo
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- CN113109306A CN113109306A CN202110354451.5A CN202110354451A CN113109306A CN 113109306 A CN113109306 A CN 113109306A CN 202110354451 A CN202110354451 A CN 202110354451A CN 113109306 A CN113109306 A CN 113109306A
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- visible light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/34—Sorting according to other particular properties
- B07C5/342—Sorting according to other particular properties according to optical properties, e.g. colour
- B07C5/3425—Sorting according to other particular properties according to optical properties, e.g. colour of granular material, e.g. ore particles, grain
- B07C5/3427—Sorting according to other particular properties according to optical properties, e.g. colour of granular material, e.g. ore particles, grain by changing or intensifying the optical properties prior to scanning, e.g. by inducing fluorescence under UV or x-radiation, subjecting the material to a chemical reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a sorting device with an aflatoxin detection function, which comprises a material slide way, wherein visible light LEDs and ultraviolet LEDs are arranged on two sides of the tail end of the material slide way, the visible light LEDs and the ultraviolet LEDs are alternately illuminated in a time-sharing manner, light splitting devices are arranged on two sides of the visible light LEDs and the ultraviolet LEDs, visible light gray-scale linear array cameras are arranged on two sides and above the light splitting devices, and two visible light gray-scale linear array cameras on the same side form a visible light gray-scale linear array camera set. The visible light LED and the ultraviolet LED are alternately illuminated in a time-sharing manner through a modulation mode, and a visible light image and a fluorescence image which are not interfered with each other are obtained; meanwhile, the fluorescence signal collecting light path is the same light path in front of the light splitting device, so that two fluorescence images for detecting aflatoxin always originate from the same position of the material, and the degree of the pollution of the material by the aflatoxin is accurately reflected; during sorting decision, the color information, the shape information and the aflatoxin detection information of the materials are integrated, and the precision and the efficiency of sorting the materials are improved.
Description
Technical Field
The invention relates to the field of sorting devices, in particular to a sorting device with an aflatoxin detection function and a sorting method.
Background
Aflatoxins are heterocyclic compounds produced by aspergillus flavus and aspergillus parasiticus, have much higher toxicity than cyanide, arsenide and organic pesticides, and have strong carcinogenic effect. Aflatoxin is easy to pollute agricultural products such as grains and oils, food, feed and the like, directly or indirectly enters the human food chain, is one of the major problems affecting the quality of the agricultural products and the food safety, and is greatly concerned by countries in the world.
The aflatoxin can be detected by using an ultraviolet induced fluorescence method, materials polluted by the aflatoxin can generate yellow green fluorescence (BGYF) under 365nm ultraviolet irradiation, and when different materials are infected with the aflatoxin, the fluorescence displayed under the ultraviolet irradiation is different. The detection method is different from common chemical detection methods, does not need to damage materials, has short detection time and can realize online detection.
Most of the existing conventional sorting equipment adopts visible light to sort materials, and has good sorting effect on the materials with obvious color and shape difference according to the optical characteristics (reflection and transmission characteristics) of the materials, but the appearance of the materials partially polluted by the aflatoxin is not greatly different from that of normal materials, and the visible light sorting equipment is difficult to achieve the effect of removing the aflatoxin.
The invention provides a sorting device with an aflatoxin detection function on the basis, which combines visible light sorting and aflatoxin detection sorting, and detects whether the material is polluted by aflatoxin while identifying impurities and defective materials. The device has high-efficiency aflatoxin detection capability, and meets the requirements of sorting equipment on precision and yield.
According to the invention patent 2013101061046, an ultraviolet induced fluorescence method is adopted for aflatoxin detection and sorting, ultraviolet light sources with central wavelengths of 365nm distributed on two sides of the tail end of a material slide way irradiate the surface of the material to excite fluorescence on the surface of the material, a three-linear array TDI CCD sensor is used for collecting R, G, B fluorescence signals of the same material at the same position, in the implementation scheme, a light splitting prism is adopted for dividing the fluorescence signals into R, G, B three paths of signals, three single-linear array TDI CCD image sensors respectively receive R, G, B three paths of fluorescence signals, detection and recognition of aflatoxin are achieved by utilizing the difference of fluorescence colors, and sorting of the material is further achieved.
Different from a sorting device for single detection of aflatoxin, the embodiment provided by the invention patent 2015100926300 adopts a crawler-type conveying mechanism, and a common visible light image of a material and a fluorescence image passing through two optical filters with different wavelengths are simultaneously acquired at the tail end of the crawler-type conveying mechanism, so that the device is used for detecting the appearance of the material and the aflatoxin. When detecting aflatoxin, the RGB color and Normalized Difference Fluorescence Index (NDFI) of the material are used as sample information, firstly, the three materials with different pollution degrees are classified and trained by a support vector machine, then, the materials are detected and sorted by a trained model, and the materials are divided into qualified products and unqualified products according to the appearance color and the pollution degree of the aflatoxin.
Because the sensitivity and the signal-to-noise ratio of the TDI CCD image sensor are high, the TDI CCD image sensor can be used for effectively detecting weak fluorescent signals on the surface of a material, but a synthesized RGB image can only be used for detecting the existence of aflatoxin, and secondary separation is required to be carried out on impurities and defect particles contained in the material through visible light separation equipment. In addition, the TDI CCD image sensor is expensive, which directly increases the manufacturing cost of the sorting device, and at the same time, when the TDI CCD image sensor is used, it is required that the line scanning frequency is strictly synchronized with the moving rate of the object.
The wave band of the fluorescence signal generated by the ultraviolet induced fluorescence method is positioned in the visible light region. Because the visible LED and the ultraviolet LED are adopted for lighting at the same time, when a camera collects a fluorescence signal, the visible LED lighting signal and the fluorescence signal cannot be distinguished, and the detection of aflatoxin is influenced. When detecting aflatoxin by using normalized difference fluorescence index, gray signals output by a camera after passing through two wavelength filters at the same position on a material need to be obtained, in the prior patent, two flat mirrors are used on a light path to reflect fluorescent signals at the same position on the material, the success or failure of the detection mode is related to the space position of falling of the material, and in the implementation process, two independent receiving light paths cannot ensure that the obtained fluorescent images with two wavelengths always originate from the same position on the material, which has influence on judging whether the material is polluted by the aflatoxin.
Disclosure of Invention
The invention aims to provide a sorting device with an aflatoxin detection function and a sorting method, so as to solve the technical problems.
In order to solve the technical problems, the invention adopts the following technical scheme:
the sorting device with the aflatoxin detection function comprises a material slide way, visible light LEDs and ultraviolet LEDs are arranged on two sides of the tail end of the material slide way, the visible light LEDs and the ultraviolet LEDs illuminate alternately in a time-sharing mode, light splitting devices are arranged on two sides of the visible light LEDs and two sides of the ultraviolet LEDs, visible light gray-scale linear arrays are arranged on two sides and above the light splitting devices, two visible light gray-scale linear arrays on the same side form a visible light gray-scale linear array camera set, and a central wavelength lambda is arranged in front of each of the two visible light gray-scale linear arrays on the same side1Optical filter and center wavelength lambda2And visible light color linear cameras are further arranged on two sides of the light splitting device.
Preferably, the central wavelength of the ultraviolet LED is 365 nm.
Preferably, the scanning frequency of the visible light gray-scale line camera and the visible light color line camera is above kHz.
Preferably, the visible light gray scale line camera and the visible light color line camera are CCD line cameras or CMOS line cameras.
Preferably, the light splitting device is a dichroic mirror or an optical beam splitter.
Preferably, the visible light gray-scale line camera is mounted on a camera fixing support, and the light splitting device is mounted on a light splitting device fixing support.
A sorting method of a sorting device with an aflatoxin detection function comprises the following steps:
when the material particles fall along the slide way and are separated from the slide way, the visible light LED and the ultraviolet LED alternately illuminate the material in a time-sharing manner to obtain a visible light image and a fluorescence image which are not interfered with each other;
the ultraviolet LED excites fluorescence on the surface of the material, the fluorescence signals pass through two optical filters with specific central wavelengths and then are output by a visible light gray-scale linear array camera to form two fluorescence images with different wavelengths, the normalized difference fluorescence index of each pixel point is calculated, the aflatoxin is detected, and meanwhile, the materials are comprehensively analyzed and sorted according to the color and shape information of the materials in the visible light images.
The invention has the beneficial effects that:
compared with the prior art, the visible light LED and the ultraviolet LED are alternately illuminated in a time-sharing manner through a modulation mode, and a visible light image and a fluorescence image which are not interfered with each other are obtained; meanwhile, the fluorescence signal collecting light path is the same light path in front of the light splitting device, so that two fluorescence images for detecting aflatoxin always originate from the same position of the material, and the degree of the pollution of the material by the aflatoxin is accurately reflected; during sorting decision, the color information, the shape information and the aflatoxin detection information of the materials are integrated, and the precision and the efficiency of sorting the materials are improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a diagram of an integrated design structure of a light splitting device, a light filter and a visible light gray-scale line array camera set according to the present invention;
reference numerals: 1-material; 2-a material slideway; 3-visible light LEDs; 4-ultraviolet LED; 5-a light splitting device; 6-center wavelength λ1An optical filter; 7-center wavelength λ2An optical filter; 8-visible light gray scale line array camera; 9-visible light color line-array camera; 10-camera fixing bracket; 11-the fixing support of the light splitting device.
Detailed Description
In order to make the technical means, the original characteristics, the achieved purposes and the effects of the invention easily understood, the invention is further described below with reference to the specific embodiments and the attached drawings, but the following embodiments are only the preferred embodiments of the invention, and not all embodiments. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention.
Specific embodiments of the present invention are described below with reference to the accompanying drawings.
As shown in fig. 1-2, a sorting device with aflatoxin detection function comprises a material slide 2, both sides of the tail end of the material slide 2 are provided with a visible light LED3 and an ultraviolet LED4, the visible light LED3 and the ultraviolet LED4 alternately illuminate in a time-sharing manner, both sides of the visible light LED3 and the ultraviolet LED4 are provided with light splitting devices 5, both sides and the upper part of each light splitting device 5 are provided with a visible light gray-scale line array camera 8, the two visible light gray-scale line array cameras 8 on the same side form a visible light gray-scale camera set 12, and the front of the two visible light gray-scale line array cameras 8 on the same side are respectively provided with a central wavelength λ1Filter 6 and center wavelength lambda2And visible light color line cameras 9 are arranged on the two sides of the optical filter 7 and the light splitting device 5.
An ultraviolet LED4 with a central wavelength of 365nm is selected, and in the preferred embodiment of the invention, an ultraviolet LED4 with a small divergence angle is used for manufacturing the light bar. The visible light LED3 and the ultraviolet LED4 are arranged on two sides of the imaging position of the material 1 at the tail end of the material slide way 2, and the distance between the light source and the imaging position of the material 1 is reduced to improve the fluorescence intensity in consideration of the divergence characteristic of illumination of the ultraviolet LED 4. Through a modulation mode, the visible light LED3 and the ultraviolet LED4 realize time-sharing alternate illumination.
Because the visible light images and the fluorescence images can not finish collecting complete information of materials when the linear array camera alternately collects images in a time-sharing manner, in order to reduce the influence of data loss on the sorting effect, a visible light color linear array camera 9 and a visible light gray scale linear array camera 8 with high line scanning frequency are adopted, the line scanning frequency is above 25kHz, and the linear array camera can be a CCD linear array camera or a CMOS linear array camera. The modulation frequency of the LED is the same as the line scanning frequency of the linear array camera, when the visible light LED3 illuminates, the visible light color linear array camera shoots RGB images of materials, and the visible light gray scale linear array camera for collecting fluorescent signals does not work; when the ultraviolet LED4 lights, the visible light gray-scale line camera 8 collects fluorescent signals, and the visible light color line camera 9 does not work.
The generated fluorescent signal is subjected to light splittingThe optical signal is divided into two paths of mutually perpendicular optical signals after being placed 5, the light splitting device 5 can be a dichroic mirror or an optical beam splitter, and in the preferred embodiment of the invention, the dichroic mirror is adopted. According to the central wavelength lambda of the selected filter1And λ2And determining the parameters of the dichroic mirror.
The light splitting device 5, the optical filter and the visible light gray-scale linear array camera set 12 adopt an integrated design, as shown in figure 2, the light splitting device 5 and the central wavelength lambda are included1 Optical filter 6, center wavelength lambda2The device comprises an optical filter 7, a visible light gray-scale linear array camera set 12, a camera fixing support 10 and a light splitting device fixing support 11. The fluorescent signal is divided into two paths of mutually perpendicular fluorescent signals after passing through the light splitting device 5, and enters the visible light linear array gray scale camera 8 after passing through the optical filter. The visible light gray-scale linear array camera set 12 consists of two visible light gray-scale linear array cameras 8 with optical axes perpendicular to each other, and a specific wavelength lambda is arranged in front of each camera1And λ2The camera outputs fluorescent images with two wavelengths, the gray values of matched pixel points in the fluorescent images are b1 and b2 respectively, and the normalized difference fluorescence index NDFI of each pixel point is calculated as (b1-b2)/(b1+ b 2).
By analyzing the spectrums of the materials polluted by aflatoxin in different degrees, the central wavelength of the selected optical filter can realize effective distinguishing of whether the materials are polluted or not. For example, for corn, the aflatoxin detection can be performed by adopting two wavelengths of 437nm and 537nm, and the calculated normalized difference fluorescence index value is directly related to the content of the aflatoxin in the material.
During online detection and sorting, the detection of the aflatoxin pollution degree is realized by the normalized difference fluorescence index of each pixel point, and meanwhile, the materials are comprehensively analyzed and sorted according to the color and shape information of the materials in the visible light image.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. The utility model provides a sorting unit with aflatoxin detects function, includes material slide (2), its characterized in that: visible light LED (3) and ultraviolet LED (4) are arranged on two sides of the tail end of the material slide way (2), the visible light LED (3) and the ultraviolet LED (4) alternately illuminate in a time-sharing mode, light splitting devices (5) are arranged on two sides of the visible light LED (3) and the ultraviolet LED (4), visible light gray linear array cameras (8) are arranged on two sides and above the light splitting devices (5), the two visible light gray linear array cameras (8) on the same side form a visible light gray linear array camera set (12), and central wavelength lambda is arranged in front of the two visible light gray linear array cameras (8) on the same side respectively1Filter (6) and center wavelength lambda2And the two sides of the light splitting device (5) are also provided with visible light color line cameras (9).
2. The sorting device with aflatoxin detection function according to claim 1, characterized in that: the central wavelength of the ultraviolet LED (4) is 365 nm.
3. The sorting device with aflatoxin detection function according to claim 1, characterized in that: the scanning frequency of the visible light gray-scale linear array camera (8) and the visible light color linear array camera (9) is above 25 kHz.
4. The sorting device with aflatoxin detection function according to claim 1, characterized in that: the visible light gray scale linear array camera (8) and the visible light color linear array camera (9) are CCD linear array cameras or CMOS linear array cameras.
5. The sorting device with aflatoxin detection function according to claim 1, characterized in that: the light splitting device (5) is a dichroic mirror or an optical beam splitter.
6. The sorting device with aflatoxin detection function according to claim 1, characterized in that: the visible light gray scale linear array camera (8) is installed on a camera fixing support (10), and the light splitting device (5) is installed on a light splitting device fixing support (11).
7. A sorting method of a sorting device with aflatoxin detection function is characterized in that: the method comprises the following steps:
when the material particles fall along the slide way and are separated from the slide way, the visible light LED and the ultraviolet LED alternately illuminate the material in a time-sharing manner to obtain a visible light image and a fluorescence image which are not interfered with each other;
the ultraviolet LED excites fluorescence on the surface of the material, the fluorescence signals pass through two optical filters with specific central wavelengths and then are output by a visible light gray-scale linear array camera to form two fluorescence images with different wavelengths, the normalized difference fluorescence index of each pixel point is calculated, the aflatoxin is detected, and meanwhile, the materials are comprehensively analyzed and sorted according to the color and shape information of the materials in the visible light images.
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Citations (6)
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| CN202984137U (en) * | 2012-12-27 | 2013-06-12 | 遵义师范学院 | Device for sorting grains polluted by aflatoxin B1 |
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| WO2013185317A1 (en) * | 2012-06-14 | 2013-12-19 | 中国科学院自动化研究所 | Multispectral imaging system and method based on two cameras |
| CN104646315A (en) * | 2015-03-02 | 2015-05-27 | 青岛农业大学 | Intelligent agricultural product sorting machine with aflatoxin detection function |
| CN108478196A (en) * | 2018-05-25 | 2018-09-04 | 北京数字精准医疗科技有限公司 | Spectroscopic imaging device based on molecular image |
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2021
- 2021-04-01 CN CN202110354451.5A patent/CN113109306A/en active Pending
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| WO2013185317A1 (en) * | 2012-06-14 | 2013-12-19 | 中国科学院自动化研究所 | Multispectral imaging system and method based on two cameras |
| CN202984137U (en) * | 2012-12-27 | 2013-06-12 | 遵义师范学院 | Device for sorting grains polluted by aflatoxin B1 |
| CN203148850U (en) * | 2013-03-29 | 2013-08-21 | 合肥美亚光电技术股份有限公司 | Aflatoxin toxin detecting and sorting device |
| CN104646315A (en) * | 2015-03-02 | 2015-05-27 | 青岛农业大学 | Intelligent agricultural product sorting machine with aflatoxin detection function |
| CN108478196A (en) * | 2018-05-25 | 2018-09-04 | 北京数字精准医疗科技有限公司 | Spectroscopic imaging device based on molecular image |
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