CN113109224A - Biological aerosol early warning sampling and detection integrated system and method - Google Patents
Biological aerosol early warning sampling and detection integrated system and method Download PDFInfo
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Abstract
The invention belongs to the technical field of monitoring and early warning of bioaerosol, and particularly relates to a bioaerosol early warning sampling and detecting integrated system and a method, which have the function of quickly obtaining bioaerosol types, wherein the system comprises an early warning device, a sampling device, a detecting device and a control device; the early warning device monitors the concentration of bioaerosol in the environment and transmits the monitoring result to the control device; when the concentration value of the bioaerosol exceeds a set threshold value, the control device triggers the sampling device and the detection device to start working; the sampling device starts to collect a bioaerosol sample under the triggering of the control device; the sampling device is provided with a cleaning module, and when the system is in a cleaning mode, the cleaning module cleans the sampling device; the detection device is triggered by the control device and is used for detecting the sampled bioaerosol sample, and transmitting a detection result to the control device, uploading the detection result to the server and/or uploading the detection result to the cloud.
Description
Technical Field
The invention belongs to the technical field of monitoring and early warning of bioaerosol, and particularly relates to a bioaerosol early warning sampling and detection integrated system and method.
Background
In recent years, SARS, Ebola, influenza and new crown epidemic situation spread widely in the global scope, seriously endanger the human life health and impact the global politics and economy. Since viruses can be transmitted through aerosol, the bioaerosol transmission risk monitoring and prevention control needs to be enhanced, and particularly, the bioaerosol automatic monitoring early warning and sampling detection integrated system and method are needed.
Currently, the common methods for bioaerosol monitoring are mie scattering and fluorescence scattering, wherein the mie scattering method is used for measuring the size and the quantity of particles, and the fluorescence spectrum method is used for identifying biological particles. The principle is as follows: when ultraviolet/violet laser light is incident on a particulate material, mie scattering occurs at the same wavelength as the incident light, and if the particle is a biological particle, it is excited to emit fluorescence at a wavelength longer than the laser light. The Mie scattered light and the fluorescence signals are respectively measured by the photoelectric detector, so that whether the particles are biological particles or non-biological particles can be distinguished; the number of light pulses recorded by the detector corresponds to the number of particles, so that the concentration of the particles can be measured; the intensity of the light pulse recorded by the detector reflects the intensity of the mie scattering, so that the size of the particles can be estimated. The intensity of mie scattering is not only related to the size of the particles, but also affected by the shape, material, refractive index, etc. of the particles.
The laser excitation fluorescence spectrum is called fluorescence for short, and refers to a fluorescence spectrum, wherein a graph of the relationship between the energy and the wavelength of the fluorescence is the fluorescence spectrum. The laser is usually visible light or near ultraviolet light.
Generally, the field rapid detection means of the virus mainly adopts a colloidal gold detection card. In 1971, Faulk and Taytor introduce colloidal gold into human immunochemistry, and the waiting immune colloidal gold technology is increasingly widely applied in various biomedical fields as a new immunological method, and the current applications in detection mainly comprise immunochromatography (immunochromatography-phy) and rapid immunogold filtration assay (DIGFA) for detecting HBsAg, HCG, anti-double-chain DNA antibodies and the like, and have the advantages of simplicity, rapidness, accuracy, no pollution and the like.
The exuberant patent of 'a system and a method for monitoring bioaerosol in real time' indicates that the silicon nanowire biosensor can realize real-time online monitoring of bioaerosol, but is difficult to widely popularize and apply due to the reasons of accuracy, stability and the like of the sensor. Currently, no product is practical to use that can identify bioaerosols. At present, global new crown blight is too heavily abused, bioaerosol threatens environment and human health seriously, and bioaerosol monitoring, early warning, sampling and detecting integrated technical equipment is urgently needed to be put into practical use. As the optical technology, the immunological technology and the nucleic acid detection technology are mature and products thereof are widely applied, the technology has important significance in monitoring and early warning of the bioaerosol.
Disclosure of Invention
The embodiment of the invention provides a bioaerosol early warning sampling and detecting integrated system and method, which can not only carry out bioaerosol monitoring and early warning and quickly collect aerosol samples, but also have the function of quickly discriminating bioaerosol types.
In a first aspect, an embodiment of the present application provides a bioaerosol early warning sampling and detection integrated system, including: the device comprises an early warning device, a sampling device, a detection device and a control device;
the early warning device is used for monitoring the concentration of bioaerosol in the environment and transmitting the monitoring result to the control device;
the control device is used for triggering the sampling device and the detection device to start working when the concentration value of the bioaerosol exceeds a set threshold value;
the sampling device starts to collect a bioaerosol sample under the triggering of the control device; the sampling device is provided with a cleaning module, and when the system is in a cleaning mode, the cleaning module cleans the sampling device;
the detection device detects the sampled bioaerosol sample under the trigger of the control device, transmits the detection result to the control device and uploads the detection result to the server.
Optionally, the cleaning module is composed of a heating module and a disinfection spray, the disinfection spray is located at an air inlet of the sampling device and used for cleaning and disinfecting the whole sampling device and a transfusion pipeline thereof, the heating module is used for heating the sampling device, and when the system is in a cleaning mode, wall-hanging water drops staying in the module are removed.
Optionally, the sampling device mainly comprises a sampling module, a fluid infusion peristaltic pump, a reagent bottle, a fluid outlet peristaltic pump, a short sliding table, a fluid outlet needle and a detection card; wherein the sampling module collects aerosol particles of the environment by a negative pressure spiral impact method; the reagent bottle is connected with the sampling module through an input liquid guide pipe, and the liquid supplementing peristaltic pump is positioned on the input liquid guide pipe; the sampling module is connected with the short sliding table through an output liquid guide pipe, the liquid outlet peristaltic pump is positioned on the output liquid guide pipe, the liquid outlet needle is connected to the short sliding table, and the liquid outlet needle is used for leading out the collected liquid collected by the sampling module to the collecting card.
Furthermore, the collection device is also provided with a waste liquid bottle for collecting waste liquid generated during the cleaning of the system.
Furthermore, sampling device still includes long slip table, detection card and waste liquid bottle are sealed completely to can remove about under the drive of long slip table, thereby realize will delivering the detection card of passing through liquid to the detection module in the region that the test probe can detect.
Furthermore, the system also comprises an automatic loading detection card device used for automatically loading the detection card onto the long sliding table, and a detection card recovery device used for automatically recovering the detection card after the detection of the detection card by the detection device is finished.
Optionally, the detection device sends the detection result to the control device, and the control device analyzes the type and concentration of the bioaerosol by combining the information sent by the early warning device and the detection device to determine whether to send an alarm.
Optionally, the long sliding table, the automatic loading detection card device and the detection card recovery device are controlled by a control device.
Alternatively, the detection card can be made by an enzyme-linked immunosorbent assay.
In a second aspect, an embodiment of the present application provides a bioaerosol early warning sampling and detection integrated method, which specifically comprises the following processes:
monitoring the concentration of bioaerosol in the environment by using an early warning device, and transmitting the monitoring result to a control device;
when the control device judges that the concentration value of the bioaerosol exceeds a set threshold value, triggering the sampling device and the detection device to start working;
under the trigger of the control device, the sampling device starts to collect a bioaerosol sample;
under the trigger of the control device, the detection device detects the sampled bioaerosol sample, transmits the detection result to the control device and uploads the detection result to the server;
after the detection is finished, the cleaning module cleans the sampling device under the control of the control device.
Advantageous effects
The system is provided with the early warning device and the sampling device, so that the early warning sampling and detection integrated design of the bioaerosol is realized, and meanwhile, the sampling device is also provided with the cleaning module, when the system is in a cleaning mode, the cleaning module cleans the sampling device, so that the influence of residual liquid left in the sampling device after last sampling on a result of secondary sampling is avoided, the high accuracy of the sampling result is ensured, the detection card can be automatically assembled and disassembled, and the continuous work of integrated equipment is realized.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a bioaerosol early warning sampling and detecting device of the present invention.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
It should be noted that, in the case of no conflict, the features in the following embodiments and examples may be combined with each other; moreover, all other embodiments that can be derived by one of ordinary skill in the art from the embodiments disclosed herein without making any creative effort fall within the scope of the present disclosure.
It is noted that various aspects of the embodiments are described below within the scope of the appended claims. It should be apparent that the aspects described herein may be embodied in a wide variety of forms and that any specific structure and/or function described herein is merely illustrative. Based on the disclosure, one skilled in the art should appreciate that one aspect described herein may be implemented independently of any other aspects and that two or more of these aspects may be combined in various ways. For example, an apparatus may be implemented and/or a method practiced using any number of the aspects set forth herein. Additionally, such an apparatus may be implemented and/or such a method may be practiced using other structure and/or functionality in addition to one or more of the aspects set forth herein.
As shown in fig. 1, an embodiment of the present application provides a bioaerosol early warning sampling and detection integrated system, which includes an early warning device, a sampling device, a detection device, and a control device.
The early warning device is used for monitoring the concentration of bioaerosol in the environment and transmitting the monitoring result to the control device.
In specific implementation, the early warning device can be realized by adopting a laser-induced fluorescence technology, wherein the laser-induced fluorescence technology is that after a substance is irradiated by a laser light source with a certain wavelength, molecules acquire energy and are excited from a ground state to an excited state, and the excited state belongs to an unstable state, and the molecules jump back to the ground state in a short time to emit fluorescence with a wavelength larger than that of incident light; therefore, the early warning device can monitor the bioaerosol in the environment and distinguish the non-biological aerosol and the biological aerosol in the air to obtain the concentration of the bioaerosol, and the concentration is transmitted to the control device. The early warning device adopts a laser bioaerosol counting mode, and has the advantages of high accuracy, no need of concentration and separation, capability of carrying out long-time automatic real-time monitoring and the like. Meanwhile, the early warning module adopts a single laser as a light source, simultaneously monitors the optical equivalent particle size and the fluorescence intensity of aerosol particles, structurally, the elastic scattering light measurement light path and the fluorescence measurement light path share the same light path, and the early warning module has the advantages of small volume and simple structure.
The control device is used for triggering the sampling device and the detection device to start working when the concentration value of the bioaerosol exceeds a set threshold value.
When the method is specifically implemented, a bioaerosol concentration threshold value is stored in the control device in advance, the threshold value can be set manually, and different threshold values can be set according to different use conditions. The early warning device collects the concentration of the bioaerosol in the environment and transmits the concentration back to the control device, the control device compares the received concentration value with a pre-stored threshold value, when the received concentration value exceeds the threshold value, the concentration of the bioaerosol sample in the air is indicated to be high at the moment, and further sampling detection is necessary, so that when the concentration of the bioaerosol in the air exceeds the set threshold value, the control device controls the early warning device to send out an early warning signal on one hand, and on the other hand, the adoption device and the detection device are promoted to start working.
The sampling device starts to collect a bioaerosol sample under the triggering of the control device; and the sampling device is provided with a cleaning module, and when the system is in a cleaning mode, the cleaning module cleans the sampling device.
In specific implementation, the sampling device is in a dormant state when being electrified and starts to work after being triggered by the control device; the sampling device can collect aerosol particles of the environment by a negative pressure spiral impact method. The adoption duration of the adoption device can be set, and when the sampling duration reaches the set duration, the adoption device enters the sleep state again. The sampling duration of the sampling device can be preset in the control device, and when the control device monitors that the sampling duration of the sampling device reaches the preset duration, the sampling device is triggered to enter a dormant state, namely the device is controlled to be turned on and off by the control device. Meanwhile, after the sampling of the sampling device is finished, the control device controls the cleaning module to work, so that the system enters a cleaning mode, namely the cleaning module cleans the sampling device, and residual liquid in the sampling device is removed to pollute the next sampling.
The detection device is triggered by the control device and is used for detecting the sampled bioaerosol sample, transmitting the detection result to the control device and uploading the detection result to the server.
In specific implementation, the detection device is realized by adopting a multi-channel bioaerosol detection module and can be used for identifying the type of bioaerosol. Particularly, the detection module adopts a multi-channel immunochromatography detection system based on a fluorescence detection principle, and the working principle is as follows: the detected substance in the sample liquid can generate specific immunoreaction with the corresponding antibody, and after the reaction is finished, the fluorescence detection module is used for detecting the test strip, so that the qualitative or quantitative result of the detected substance in the sample liquid to be detected is obtained, and the type of the bioaerosol contained in the air is judged. The detection device is based on an immunochromatography method, can quickly identify the type of the bioaerosol, and has the advantages of short identification time and high accuracy. The detection device sends the analysis result to the control module and uploads the analysis result to the server.
Through setting up early warning device and sampling device in this application embodiment to realize the integration of early warning and sampling, have advantages such as high efficiency, quick, accuracy. Simultaneously, set up the washing module on sampling device, after sampling at every turn, the system gets into clean mode for wash sampling device, clear away this sampling process after in sampling device the liquid of residuary, in order to cause the interference to the result of sampling next time, influence the accuracy of sampling result next time, consequently this system is one kind not only can carry out biological aerosol monitoring early warning, gather the aerosol sample fast, and have the quick system that accurately differentiates the function of the biological aerosol kind of monitoring.
In one possible implementation mode, the sampling device mainly comprises a sampling module, a liquid supplementing peristaltic pump, a reagent bottle, a liquid outlet peristaltic pump, a short sliding table, a liquid outlet needle and a detection card; wherein the sampling module collects aerosol particles of the environment by a centrifugal method; the reagent bottle is connected with the sampling module through an input liquid guide pipe, and the liquid supplementing peristaltic pump is positioned on the input liquid guide pipe; the sampling module is connected with the short sliding table through an output liquid guide pipe, the liquid outlet peristaltic pump is positioned on the output liquid guide pipe, the liquid outlet needle is connected to the short sliding table, and the liquid outlet needle is used for leading out the collected liquid collected by the sampling module to the collecting card.
During specific implementation, when the control device judges that the concentration of the gas bioaerosol in the air collected at present is greater than a set threshold value, the sampling device is triggered to start working, the liquid supplementing peristaltic pump is started at the moment, liquid in the reagent bottle is input into the sampling module, the sampling module starts to collect an air bioaerosol sample, and then the liquid outlet peristaltic pump outputs the collected liquid in the sampling module to the detection card through the infusion needle. The process is automatically completed under the control of the control module, the implementation efficiency is high, the automation capacity is high, and the intervention and intervention of personnel are reduced. Meanwhile, the sampling module collects aerosol particles in the environment by a high-flow negative-pressure spiral impact method, and has the characteristics of high flow, no need of enrichment, high collection efficiency and the like.
In a possible embodiment, the cleaning module is composed of a heating module and a sterilization spray, the sterilization spray is located at an air inlet of the sampling device and is used for cleaning and sterilizing the whole sampling device and a transfusion pipeline thereof, the heating module is used for heating the sampling device, and when the system is in a cleaning mode, wall-hanging water drops staying in the module are removed.
When the detection is finished, the control device controls the disinfection spray and the sampling device to be started, the disinfection spray is used for cleaning the sampling device, and the cleaning liquid is discharged to a waste liquid bottle by a liquid outlet peristaltic pump after the cleaning is finished; the control device starts the heating module and starts to dry the sampling device; then the liquid is replenished by a liquid replenishing peristaltic pump, the infusion pipeline is cleaned again, and finally the infusion liquid is discharged to a waste liquid bottle. Through the arrangement of the disinfection spray and the heating module, when the system enters a cleaning mode after sampling is finished, the sampling module can be thoroughly sampled, so that pollution caused by subsequent sampling is avoided.
In a possible embodiment, the collecting device is further provided with a waste bottle. During specific implementation, after the collection of the bioaerosol sample in air is finished, the control device control system enters the sampling device to enter a cleaning mode, the sampling module is cleaned by disinfection spraying work, and the cleaned liquid is discharged to a waste liquid bottle by the liquid outlet peristaltic pump after the cleaning is finished.
In a possible implementation mode, the sampling device further comprises a long sliding table, the detection card and the waste liquid bottle are completely sealed and can move left and right under the driving of the long sliding table, and therefore the detection card which is conveyed with liquid can be conveyed to an area which can be detected by a detection probe of the detection module. Through sealing the detection card completely, the waste liquid bottle is sealed completely, and secondary volatilization of aerosol can be avoided.
In a possible implementation mode, the system further comprises an automatic loading detection card device used for automatically loading the detection card onto the long sliding table, and the system further comprises a detection card recovery device, when the detection card is detected by the detection device, the detection card is automatically recovered by the detection card recovery device, and after the detection card is uniformly recovered, the detection card is uniformly processed. The automatic detection card loading device can load multi-channel detection card rows for multiple times, and the detection card recovery device can also recover the detection cards for multiple times; the manual intervention in the using process of the system is reduced, and the full-automatic continuous operation of the system is realized.
In a possible implementation mode, the detection device sends the detection result to the control device, and the control device analyzes the type and the concentration of the bioaerosol by combining the information transmitted by the early warning device and the detection device to judge whether to send out an alarm or not. For example, when the bioaerosol in the air is harmful aerosol to human bodies and has high concentration, an alarm signal is sent.
In a possible embodiment, the long slide table, the automatic loading detection card device and the detection card recovery device are controlled by a control device. When the device is specifically implemented, the control device respectively controls the early warning device, the detection device, the sampling module, the heating module, the disinfection spray, the liquid supplementing peristaltic pump, the liquid outlet peristaltic pump, the long sliding table, the short sliding table, the automatic filling detection card device and the detection card recovery device, when the concentration of the bioaerosol monitored by the early warning device exceeds a threshold value, the control device sends out an alarm signal, simultaneously triggers the sampling module, the liquid supplementing peristaltic pump and the liquid outlet peristaltic pump to start collecting bioaerosol samples, controls the long sliding table and the short sliding table to drive the infusion needle, and carries out linkage operation on the detection card and the waste liquid bottle, detects the detection card when the detection card is sent to the detection device, and finally sends the detected detection card to the detection card recovery module.
Optionally, the input catheter and the output catheter both adopt medical silicone tubes, and the medical silicone tubes are strong in antibacterial capacity, relatively soft and convenient to use.
Alternatively, the detection card can be made by an enzyme linked immunosorbent assay.
The embodiment of the invention can set the particle size of the early warning monitoring particles and the warning threshold value; the sampling time can be set; time interval between two detections and repeated detection times can be set, the cleaning times are set, one key is started, unattended operation and full-automatic intelligent operation are realized, all parts of the system can be detached, disinfection treatment is carried out, and secondary pollution is avoided.
As shown in fig. 1, the embodiment of the present application provides an integrated method for early warning sampling and detection of bioaerosol, which comprises the following specific processes:
monitoring the concentration of bioaerosol in the environment by using an early warning device, and transmitting the monitoring result to a control device;
when the control device judges that the concentration value of the bioaerosol exceeds a set threshold value, triggering the sampling device and the detection device to start working;
under the trigger of the control device, the sampling device starts to collect a bioaerosol sample;
under the trigger of the control device, the detection device detects the sampled bioaerosol sample, and transmits the detection result to the control device, uploads the detection result to the server and/or uploads the detection result to the cloud;
after the detection is finished, the cleaning module cleans the sampling device under the control of the control device.
The bioaerosol early warning sampling and detecting integrated device and the bioaerosol early warning sampling and detecting integrated method provided by the embodiment of the application adopt the same invention concept, can obtain the same beneficial effect, and are not repeated herein.
The above description is only for the specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. The utility model provides a biological aerosol early warning sampling and detection integration system which characterized in that includes: the device comprises an early warning device, a sampling device, a detection device and a control device;
the early warning device is used for monitoring the concentration of bioaerosol in the environment and transmitting the monitoring result to the control device;
the control device is used for triggering the sampling device and the detection device to start working when the concentration value of the bioaerosol exceeds a set threshold value;
the sampling device starts to collect a bioaerosol sample under the triggering of the control device; the sampling device is provided with a cleaning module, and when the system is in a cleaning mode, the cleaning module cleans the sampling device;
the detection device is triggered by the control device and is used for detecting the sampled bioaerosol sample, and transmitting a detection result to the control device, uploading the detection result to the server and/or uploading the detection result to the cloud.
2. The bioaerosol early warning sampling and detection integrated system as claimed in claim 1, wherein the cleaning module is composed of a heating module and a sterilization spray, the sterilization spray is located at an air inlet of the sampling device and is used for cleaning and sterilizing the whole sampling device and an infusion pipeline thereof, the heating module is used for heating the sampling device, and when the system is in a cleaning mode, wall-hanging water drops staying in the sampling module are removed.
3. The bioaerosol early warning sampling and detection integrated system as claimed in claim 1, wherein the sampling device mainly comprises a sampling module, a liquid supplementing peristaltic pump, a reagent bottle, a liquid outlet peristaltic pump, a short sliding table, a liquid outlet needle and a detection card; wherein the sampling module collects aerosol particles of the environment in a negative pressure spiral impact manner; the reagent bottle is connected with the sampling module through an input liquid guide pipe, and the liquid supplementing peristaltic pump is positioned on the input liquid guide pipe; the sampling module is connected with the short sliding table through an output liquid guide pipe, the liquid outlet peristaltic pump is positioned on the output liquid guide pipe, the liquid outlet needle is connected to the short sliding table, and the liquid outlet needle is used for leading out the collected liquid collected by the sampling module to the collecting card.
4. The bioaerosol early warning sampling and detection integrated system as claimed in claim 1, wherein the collection device is further provided with a waste liquid bottle for collecting waste liquid generated during cleaning of the system.
5. The bioaerosol early warning sampling and detection integrated system as claimed in claim 4, wherein the sampling device further comprises a long sliding table, the detection card and the waste liquid bottle are completely sealed and can be driven by the long sliding table to move left and right, so that the detection card which is conveyed with liquid can be conveyed to an area which can be detected by the detection probe of the detection module.
6. The bioaerosol early warning sampling and detection integrated system of claim 5, further comprising an automatic loading detection card device for automatically loading the detection card onto the long sliding table, and a detection card recycling device for automatically recycling the detection card after the detection of the detection card by the detection device.
7. The bioaerosol early warning sampling and detection integrated system as claimed in claim 1, wherein the detection device sends the detection result to the control device, and the control device analyzes the type and concentration of bioaerosol and determines whether to alarm by combining the information transmitted from the early warning device and the detection device.
8. The bioaerosol early warning sampling and detection integrated system of claim 6, wherein the long sliding table, the automatic loading detection card device and the detection card recovery device are controlled by a control device.
9. The bioaerosol early warning sampling and detection integrated system as claimed in claim 1, wherein the detection card can be made by an immunization or nucleic acid detection method, and can be a single card or a multi-card.
10. A bioaerosol early warning sampling and detection integrated method is characterized by comprising the following specific processes:
monitoring the concentration of bioaerosol in the environment by using an early warning device, and transmitting the monitoring result to a control device;
when the control device judges that the concentration value of the bioaerosol exceeds a set threshold value, triggering the sampling device and the detection device to start working;
under the trigger of the control device, the sampling device starts to collect a bioaerosol sample;
under the trigger of the control device, the detection device detects the sampled bioaerosol sample, and transmits the detection result to the control device, uploads the detection result to the server and/or uploads the detection result to the cloud;
after the detection is finished, the cleaning module cleans the sampling device under the control of the control device.
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Cited By (1)
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CN114343723A (en) * | 2021-12-22 | 2022-04-15 | 北京华泰诺安技术有限公司 | System and method for non-invasive simultaneous collection of human exhaled aerosol and gas |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114343723A (en) * | 2021-12-22 | 2022-04-15 | 北京华泰诺安技术有限公司 | System and method for non-invasive simultaneous collection of human exhaled aerosol and gas |
CN114343723B (en) * | 2021-12-22 | 2022-11-22 | 北京华泰诺安技术有限公司 | System and method for non-invasive simultaneous collection of human exhaled aerosol and gas |
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