CN113104985A - Method for assisting in improving sewage treatment efficiency of bacterial-algae symbiotic system by using rare earth - Google Patents
Method for assisting in improving sewage treatment efficiency of bacterial-algae symbiotic system by using rare earth Download PDFInfo
- Publication number
- CN113104985A CN113104985A CN202110394717.9A CN202110394717A CN113104985A CN 113104985 A CN113104985 A CN 113104985A CN 202110394717 A CN202110394717 A CN 202110394717A CN 113104985 A CN113104985 A CN 113104985A
- Authority
- CN
- China
- Prior art keywords
- microalgae
- rare earth
- algae
- cylindrical container
- domestic sewage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Ecology (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for assisting in improving sewage treatment efficiency of a bacterial-algae symbiotic system by using rare earth, and relates to the field of urban domestic sewage treatment. The invention aims to solve the technical problem that the growth of microalgae in the existing microalgae symbiotic system is limited by light factors. The method comprises the following steps: firstly, domesticating microalgae species in domestic sewage; secondly, preparing a material containing Ce element; and thirdly, feeding the domesticated microalgae species and the Ce element-containing material into the domestic sewage to be treated to form a bacteria-algae symbiotic system. According to the invention, the activity of enzymes related to photosynthesis and respiration of algae is improved by adding low-concentration rare earth, so that the metabolic activity of the algae is improved, and sewage pollutants are absorbed and degraded. The invention is suitable for urban domestic sewage treatment.
Description
Technical Field
The invention relates to the field of urban domestic sewage treatment.
Background
Along with the faster and faster urbanization construction, the discharge amount of domestic sewage in China is increased sharply, and in the face of the current situation of unattractive water resources, the sewage treatment strength of China is increased continuously in recent years, and the traditional sewage treatment process generally has the problems of high energy consumption, secondary pollution, high investment cost, high content of discharged water N, P and the like. The microalgae is used for purifying domestic sewage, and sewage treatment and microalgae culture are combined, so that the sewage can be recycled, the cultured microalgae also has higher economic and social values, but the growth of the microalgae in a bacteria-algae symbiotic system is limited by light factors, and the development of a sewage treatment method with low energy consumption and good sewage treatment effect is urgently needed.
Disclosure of Invention
The invention provides a method for improving sewage treatment efficiency of a bacterial-algae symbiotic system by the aid of rare earth, aiming at solving the technical problem that microalgae growth in the existing bacterial-algae symbiotic system is limited by light factors.
A method for assisting in improving sewage treatment efficiency of a bacterial-algae symbiotic system by using rare earth comprises the following steps:
firstly, domesticating microalgae species in domestic sewage;
secondly, preparing a material containing Ce element;
thirdly, the microalgae seeds domesticated in the first step and the Ce-containing material in the second step are added into the domestic sewage to be treated to form a bacteria-algae symbiotic system, wherein the concentration of Ce ions in the system is controlled to be 0.3-1.0 mg/L, and the concentration of algae cells is controlled to be 104～107cell/mL, aeration intensity of 0.3-0.9L/min, light-dark period of 12 hours light, 12 hours dark, light intensity of 50-100 μm/(m)2S) at 25-30 ℃ and the residence time of the domestic sewage to be treated is 6-9 days, thus completing the method.
The invention has the beneficial effects that: the rare earth elements can obviously improve the net photosynthetic oxygen release activity of the microalgae, promote the transfer of light energy from PSII to PSI and promote the photosynthetic oxygen release and hydrogen release effects related to PSII. Meanwhile, the rare earth can promote the activity of antioxidant enzymes such as SOD, CAT, POD and the like in microalgae cells to be increased, and the capacity of removing active oxygen is enhanced.
Aiming at the practical problems that the growth of microalgae in a bacteria-algae symbiotic system is limited by a light factor, the operation of a microalgae reactor is unstable and the like, the invention adopts rare earth element Ce to promote the bacteria-algae symbiotic system to treat domestic sewage based on the low-dose stimulation effect of the rare earth element on the action of the microalgae. The activity of enzymes related to photosynthesis and respiration of algae is improved by adding low-concentration rare earth, so that the metabolic activity of the algae is improved, sewage pollutants are absorbed and degraded, the problem of N, P removal in urban domestic sewage is solved, the energy consumption is further reduced, the engineering application of microalgae sewage treatment is helped, and resources in the sewage are synchronously recycled.
After the rare earth elements are added, the protein content of the microalgae is increased by 30-50%, and the total chlorophyll content is increased by 30-40%. Low light intensity (less than or equal to 50 μm/(m)2S)), the removal rate of microalgae TN reaches more than 70%, the removal rate of ammonia nitrogen reaches more than 85%, the removal rate of TP reaches more than 90%, the removal rate of COD reaches more than 75%, and the illumination intensity is close to or exceeds 100 mu m/(m)2S) the effect of microalgae on the removal of pollutants from wastewater.
The invention is used for sewage treatment.
Drawings
FIG. 1 is a schematic view of the reactor according to the first embodiment.
Detailed Description
The technical solution of the present invention is not limited to the specific embodiments listed below, and includes any combination of the specific embodiments.
The first embodiment is as follows: the method for assisting in improving the sewage treatment efficiency of the phycobiont system by using the rare earth comprises the following steps of:
firstly, domesticating microalgae species in domestic sewage;
secondly, preparing a material containing Ce element;
thirdly, the microalgae seeds domesticated in the first step and the Ce-containing material in the second step are added into the domestic sewage to be treated to form a bacteria-algae symbiotic system, wherein the concentration of Ce ions in the system is controlled to be 0.3-1.0 mg/L, and the concentration of algae cells is controlled to be 104～107cell/mL, aeration intensity of 0.3-0.9L/min, light dark period of 12 hours illumination, 12 hoursDark and light intensity of 50-100 μm/(m)2S) at 25-30 ℃ and the residence time of the domestic sewage to be treated is 6-9 days, thus completing the method.
The second embodiment is as follows: the first difference between the present embodiment and the specific embodiment is: step one the domestication method is as follows: culturing microalgae species by using domestic sewage. The rest is the same as the first embodiment.
The third concrete implementation mode: the present embodiment differs from the first or second embodiment in that: step one, the microalgae is chlorella pyrenoidosa. The other is the same as in the first or second embodiment.
The fourth concrete implementation mode: the difference between this embodiment mode and one of the first to third embodiment modes is: and step two, the Ce element-containing material is Ce element compound powder, Ce element aqueous solution or Ce element filler. The others are the same as in one of the first to third embodiments.
The fifth concrete implementation mode: the difference between this embodiment and one of the first to fourth embodiments is: the Ce element compound is CeCl3·7H2And O. The other is the same as one of the first to fourth embodiments.
The sixth specific implementation mode: the difference between this embodiment and one of the first to fifth embodiments is: and in the step three, the concentration of Ce ions in the system is 0.4-1.0 mg/L. The other is the same as one of the first to fifth embodiments.
The seventh embodiment: the difference between this embodiment and one of the first to sixth embodiments is: the concentration of algae cells in the step three control system is 105～106cell/mL. The other is the same as one of the first to sixth embodiments.
The specific implementation mode is eight: the present embodiment differs from one of the first to seventh embodiments in that: and step three, carrying out aeration in the system. The other is the same as one of the first to seventh embodiments.
The specific implementation method nine: the present embodiment differs from the first to eighth embodiments in that: and the temperature of the photobioreactor in the third step is controlled to be 26-27 ℃. The others are the same as the first to eighth embodiments.
The detailed implementation mode is ten: the present embodiment differs from one of the first to ninth embodiments in that: and step three, forming a bacteria-algae symbiotic system in the photobioreactor, wherein the photobioreactor comprises a cylindrical container and a bottom bracket 10, a feeding inoculation port 1 and an exhaust port 2 are arranged at the top end of the cylindrical container, a plurality of sampling ports are arranged on the side wall of the cylindrical container from top to bottom, an illumination system 9 is arranged around the periphery of the side wall, an aeration disc 7 is arranged at the bottom of the cylindrical container, an air inlet 8 of the aeration disc is arranged outside the cylindrical container, and the lower end of the cylindrical container is fixed with the bottom bracket 10. The other is the same as one of the first to ninth embodiments.
The following examples were used to demonstrate the beneficial effects of the present invention:
the first embodiment is as follows:
the embodiment of the invention provides a method for assisting in improving sewage treatment efficiency of a phycobiont system by using rare earth, which is characterized by comprising the following steps of:
firstly, culturing chlorella pyrenoidosa (FACHB-5) by using a standard BG11 culture medium, and then taking microalgae with good growth state as a culture solution for culture and domestication;
II, adopting CeCl3·7H2O (analytically pure) and distilled water to prepare Ce3Ce element aqueous solution with the concentration of 1000 mg/L;
thirdly, feeding the microalgae domesticated in the first step and the Ce-containing material in the second step into a photobioreactor filled with domestic sewage, and controlling various parameters of the photobioreactor to form a bacteria-algae symbiotic system to finish the method;
the photobioreactor comprises a cylindrical container and a bottom bracket 10, wherein a feeding inoculation port 1 and an exhaust port 2 are arranged at the top end of the cylindrical container, four sampling ports are arranged on the side wall of the cylindrical container from top to bottom, an illumination system 9 is wound on the periphery of the side wall, an aeration disc 7 is arranged at the bottom of the cylindrical container, an air inlet 8 of the aeration disc is arranged outside the cylindrical container, and the lower end of the cylindrical container is fixed with the bottom bracket 10.
And step three, controlling the concentration of Ce ions in the photobioreactor to be 1.0 mg/L.
Step three control of photobiologyThe concentration of algae cells in the reactor is 105～106cell/mL。
And step three, controlling the aeration intensity of the photobioreactor to be 0.6L/min.
The light-dark period of the step three control photobioreactor is 12 hours of light, the light-dark period is 12 hours of darkness, and the light intensity is 50 mu m/(m)2·s)。
Step three controls the temperature of the photobioreactor to be 26 DEG C
And step three, controlling the residence time of the domestic sewage to be 9 days.
And step three, four sampling ports, namely a first sampling port 3, a second sampling port 4, a third sampling port 5 and a fourth sampling port 6 are arranged on the side wall of the cylindrical container of the photobioreactor from top to bottom.
The water quality indexes of ammonia nitrogen, total phosphorus and COD of the effluent of the reactor are measured. The removal rate of TN is 73.33%, the removal rate of ammonia nitrogen is 89.98%, the removal rate of TP is 94.53%, and the removal rate of COD is 75.55%. The protein content of the microalgae is increased by 30-50%, and the total chlorophyll content is increased by 30-40%. It was confirmed that the method of the present invention approached or exceeded the light intensity of 100. mu.m/(m)2S) the effect of microalgae on the removal of pollutants from wastewater.
Claims (10)
1. A method for assisting in improving sewage treatment efficiency of a phycobiont system by using rare earth is characterized by comprising the following steps:
firstly, domesticating microalgae species in domestic sewage;
secondly, preparing a material containing Ce element;
thirdly, the microalgae seeds domesticated in the first step and the Ce-containing material in the second step are added into the domestic sewage to be treated to form a bacteria-algae symbiotic system, wherein the concentration of Ce ions in the system is controlled to be 0.3-1.0 mg/L, and the concentration of algae cells is controlled to be 104～107cell/mL, aeration intensity of 0.3-0.9L/min, light-dark period of 12 hours light, 12 hours dark, light intensity of 50-100 μm/(m)2S) at 25-30 ℃ and the residence time of the domestic sewage to be treated is 6-9 days, thus completing the method.
2. The method of claim 1, wherein the acclimatization method in the first step is as follows: culturing microalgae species by using domestic sewage.
3. The method as claimed in claim 1, wherein the microalgae in step one is Chlorella pyrenoidosa.
4. The method of claim 1, wherein the Ce-containing material in step two is Ce compound powder, Ce aqueous solution or Ce filler.
5. The method of claim 4, wherein the Ce compound is CeCl3·7H2O。
6. The method of claim 1, wherein the concentration of Ce ions in the three-step control system is 0.4-1.0 mg/L.
7. The method of claim 1, wherein the algae cell concentration in the three-step control system is 105～106cell/mL。
8. The method for assisting in improving wastewater treatment efficiency of a phycobiont system with rare earth according to claim 1, wherein the third step is aeration in the system.
9. The method for assisting in improving wastewater treatment efficiency of a phycobiont system with rare earth according to claim 1, wherein the temperature of the photobioreactor in the third step is controlled to be 26-27 ℃.
10. The method for improving sewage treatment efficiency of a phycobiont system assisted by rare earth according to claim 1, wherein the step three is to form the phycobiont system in a photobioreactor, the photobioreactor comprises a cylindrical container and a bottom bracket (10), a feeding inoculation port (1) and an exhaust port (2) are arranged at the top end of the cylindrical container, a plurality of sampling ports are arranged on the side wall of the cylindrical container from top to bottom, an illumination system (9) is arranged around the periphery of the side wall, an aeration disc (7) is arranged at the bottom of the cylindrical container, an air inlet (8) of the aeration disc is arranged outside the cylindrical container, and the lower end of the cylindrical container is fixed with the bottom bracket (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110394717.9A CN113104985A (en) | 2021-04-13 | 2021-04-13 | Method for assisting in improving sewage treatment efficiency of bacterial-algae symbiotic system by using rare earth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110394717.9A CN113104985A (en) | 2021-04-13 | 2021-04-13 | Method for assisting in improving sewage treatment efficiency of bacterial-algae symbiotic system by using rare earth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113104985A true CN113104985A (en) | 2021-07-13 |
Family
ID=76716491
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110394717.9A Pending CN113104985A (en) | 2021-04-13 | 2021-04-13 | Method for assisting in improving sewage treatment efficiency of bacterial-algae symbiotic system by using rare earth |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113104985A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115196838A (en) * | 2022-08-11 | 2022-10-18 | 江西挺进环保科技股份有限公司 | Denitrification method of strongly-adaptive bacteria-algae immobilization system for rare earth wastewater |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3637308C1 (en) * | 1986-11-03 | 1988-06-01 | Eberhard Potratz | Nutrient mixture for increasing the rate of biodegradation of mineral oil products and its use |
| CN1944353A (en) * | 2006-10-26 | 2007-04-11 | 上海交通大学 | Seaweed amino acid rare-earth compound foliage fertilizer and its preparing method |
| US20080127364A1 (en) * | 2006-05-17 | 2008-05-29 | James Weifu Lee | Switchable photosystem-II designer algae for photobiological hydrogen production |
| CN101343111A (en) * | 2008-09-03 | 2009-01-14 | 南京大学 | Method for improving attached growth wastewater treatment biomembrane reactor efficiency |
| CN101654298A (en) * | 2009-09-14 | 2010-02-24 | 江苏省环境科学研究院 | Primer for improving biochemical and purifying efficacy of sewage and preparation method and application method thereof |
| CN102206003A (en) * | 2011-05-05 | 2011-10-05 | 江苏大学 | Preparation method of modified pumice packing for purifying sewage |
| CN106467427A (en) * | 2015-08-20 | 2017-03-01 | 武汉禾之壮农业科技有限公司 | A kind of preparation of environment-friendly highly efficient composite foliage fertilizer |
| CN106479935A (en) * | 2016-11-22 | 2017-03-08 | 南京大学 | A kind of preparation method of use in waste water treatment denitrification denitrogenation microbial inoculum |
| CN107235762A (en) * | 2016-03-29 | 2017-10-10 | 北京京农联合科技发展有限公司 | The full nutritious photosynthetic film of bion plant and its production technology |
| US20190008157A1 (en) * | 2015-09-11 | 2019-01-10 | Heliae Development Llc | Microalgae based compositions and methods for application to plants |
-
2021
- 2021-04-13 CN CN202110394717.9A patent/CN113104985A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3637308C1 (en) * | 1986-11-03 | 1988-06-01 | Eberhard Potratz | Nutrient mixture for increasing the rate of biodegradation of mineral oil products and its use |
| US20080127364A1 (en) * | 2006-05-17 | 2008-05-29 | James Weifu Lee | Switchable photosystem-II designer algae for photobiological hydrogen production |
| CN1944353A (en) * | 2006-10-26 | 2007-04-11 | 上海交通大学 | Seaweed amino acid rare-earth compound foliage fertilizer and its preparing method |
| CN101343111A (en) * | 2008-09-03 | 2009-01-14 | 南京大学 | Method for improving attached growth wastewater treatment biomembrane reactor efficiency |
| CN101654298A (en) * | 2009-09-14 | 2010-02-24 | 江苏省环境科学研究院 | Primer for improving biochemical and purifying efficacy of sewage and preparation method and application method thereof |
| CN102206003A (en) * | 2011-05-05 | 2011-10-05 | 江苏大学 | Preparation method of modified pumice packing for purifying sewage |
| CN106467427A (en) * | 2015-08-20 | 2017-03-01 | 武汉禾之壮农业科技有限公司 | A kind of preparation of environment-friendly highly efficient composite foliage fertilizer |
| US20190008157A1 (en) * | 2015-09-11 | 2019-01-10 | Heliae Development Llc | Microalgae based compositions and methods for application to plants |
| CN107235762A (en) * | 2016-03-29 | 2017-10-10 | 北京京农联合科技发展有限公司 | The full nutritious photosynthetic film of bion plant and its production technology |
| CN106479935A (en) * | 2016-11-22 | 2017-03-08 | 南京大学 | A kind of preparation method of use in waste water treatment denitrification denitrogenation microbial inoculum |
Non-Patent Citations (3)
| Title |
|---|
| 储钟稀等: "氯化铈对螺旋藻光合放氧、色素和蛋白质形成的影响", 《中国稀土学报》 * |
| 杨慧丽: "稀土元素铈对蛋白核小球藻处理生活污水效能的影响研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 陈爱美等: "稀土对藻类生物效应的研究进展", 《稀土》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115196838A (en) * | 2022-08-11 | 2022-10-18 | 江西挺进环保科技股份有限公司 | Denitrification method of strongly-adaptive bacteria-algae immobilization system for rare earth wastewater |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gao et al. | Removal of nutrients, organic matter, and metal from domestic secondary effluent through microalgae cultivation in a membrane photobioreactor | |
| CN109231467B (en) | Method for treating black and odorous water body by adopting gas lift circulation algae biofilm reactor | |
| CN101767893B (en) | Device and method for coupling producing biological oil by utilizing microalgae to deeply treating wastewater | |
| CN108467118B (en) | Method for removing nitrogen and phosphorus in aquaculture wastewater by using immobilized algae bacteria | |
| CN103910434B (en) | To exceed standard the membrane type bioreactor of nitrogen phosphorus and treatment process thereof for the treatment of natural pond liquid | |
| CN103896407B (en) | A kind of quick startup, biofilm carbon antimicrobial composition process for purifying water | |
| CN102674529A (en) | Method and special device for treating organic wastewater by combination of microbial fuel cell and microalgae culture | |
| CN104630295B (en) | A kind of method for handling city secondary waste water using immobilized microalgae and producing grease | |
| CN105174476A (en) | Activated sludge and microalgae coupled granular system for waste water treatment and establishment and operation method thereof | |
| CN105152466A (en) | Method for treating waste water of aquatic bird cultivation through utilizing microalgae | |
| CN102392052A (en) | Biogas purification method by culturing autotrophic freshwater microalgae with biogas slurry | |
| CN105859054A (en) | Device and method for electrochemically treating sewage with microorganisms and microbial film culture method | |
| CN110240367B (en) | Sewage treatment system and method for synchronous and efficient removal of carbon, nitrogen and phosphorus | |
| CN113862127A (en) | Device and method for culturing oil-producing blue algae by using sewage containing low-concentration antibiotics | |
| CN110734138B (en) | In-situ treatment method for freshwater aquaculture wastewater | |
| CN104386825B (en) | The method that the pool, a kind of lake water body in-situ is repaired | |
| CN106915867A (en) | The preparation method of breeding wastewater biochemical treatment system and its treatment byproduct and application | |
| CN113104985A (en) | Method for assisting in improving sewage treatment efficiency of bacterial-algae symbiotic system by using rare earth | |
| CN110591918A (en) | Method for producing algae-derived sugar by purifying secondary tail water or sludge supernatant in coupling mode | |
| CN113233587B (en) | Microalgae culture pond-artificial wetland coupling system and method for deep purification of sewage | |
| Khanzada et al. | Growing fresh water microalgae in high ammonium landfill leachate | |
| CN102976485B (en) | Method and device for fast cultivation of anaerobic granular sludge for treating swine wastewater | |
| CN113856624A (en) | Preparation method of microalgae-biomass charcoal immobilized ligand and method for synchronously purifying biogas slurry and biogas | |
| Fallahi et al. | Effects of simultaneous CO2 addition and biomass recycling on growth characteristics of microalgal mixed culture | |
| CN108611276A (en) | A method of recycling treatment being carried out to distillery waste water using microalgae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |