CN106479935A - A kind of preparation method of use in waste water treatment denitrification denitrogenation microbial inoculum - Google Patents
A kind of preparation method of use in waste water treatment denitrification denitrogenation microbial inoculum Download PDFInfo
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- CN106479935A CN106479935A CN201611055970.7A CN201611055970A CN106479935A CN 106479935 A CN106479935 A CN 106479935A CN 201611055970 A CN201611055970 A CN 201611055970A CN 106479935 A CN106479935 A CN 106479935A
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- denitrification
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 238000004065 wastewater treatment Methods 0.000 title claims abstract description 18
- 241000894006 Bacteria Species 0.000 claims abstract description 54
- 229910052761 rare earth metal Inorganic materials 0.000 claims abstract description 43
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 38
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 239000000725 suspension Substances 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000010802 sludge Substances 0.000 claims abstract description 15
- 239000003223 protective agent Substances 0.000 claims abstract description 11
- 206010002660 Anoxia Diseases 0.000 claims abstract description 8
- 241000976983 Anoxia Species 0.000 claims abstract description 8
- 206010021143 Hypoxia Diseases 0.000 claims abstract description 8
- 230000007953 anoxia Effects 0.000 claims abstract description 8
- 239000012531 culture fluid Substances 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 3
- 229910017604 nitric acid Inorganic materials 0.000 claims description 38
- -1 compound rare-earth Chemical class 0.000 claims description 34
- 235000013619 trace mineral Nutrition 0.000 claims description 16
- 239000011573 trace mineral Substances 0.000 claims description 16
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 15
- 239000007836 KH2PO4 Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 15
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 10
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 10
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical group [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 235000005979 Citrus limon Nutrition 0.000 claims description 8
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 7
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 7
- 239000001509 sodium citrate Substances 0.000 claims description 7
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- 229910002492 Ce(NO3)3·6H2O Inorganic materials 0.000 claims description 5
- 229920002101 Chitin Polymers 0.000 claims description 5
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 5
- 239000004471 Glycine Substances 0.000 claims description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 5
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 5
- 229910002422 La(NO3)3·6H2O Inorganic materials 0.000 claims description 5
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 5
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 5
- 229910002651 NO3 Inorganic materials 0.000 claims description 5
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- HSJPMRKMPBAUAU-UHFFFAOYSA-N cerium(3+);trinitrate Chemical compound [Ce+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O HSJPMRKMPBAUAU-UHFFFAOYSA-N 0.000 claims description 5
- 229910052927 chalcanthite Inorganic materials 0.000 claims description 5
- 229910052564 epsomite Inorganic materials 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 5
- FYDKNKUEBJQCCN-UHFFFAOYSA-N lanthanum(3+);trinitrate Chemical compound [La+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O FYDKNKUEBJQCCN-UHFFFAOYSA-N 0.000 claims description 5
- 235000019359 magnesium stearate Nutrition 0.000 claims description 5
- 239000011565 manganese chloride Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- CFYGEIAZMVFFDE-UHFFFAOYSA-N neodymium(3+);trinitrate Chemical compound [Nd+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O CFYGEIAZMVFFDE-UHFFFAOYSA-N 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 5
- YWECOPREQNXXBZ-UHFFFAOYSA-N praseodymium(3+);trinitrate Chemical compound [Pr+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O YWECOPREQNXXBZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 229910052708 sodium Inorganic materials 0.000 claims description 5
- 239000011684 sodium molybdate Substances 0.000 claims description 5
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 5
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 5
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 5
- 239000011686 zinc sulphate Substances 0.000 claims description 5
- 244000248349 Citrus limon Species 0.000 claims description 4
- 244000131522 Citrus pyriformis Species 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000648 calcium alginate Substances 0.000 claims description 4
- 235000010410 calcium alginate Nutrition 0.000 claims description 4
- 229960002681 calcium alginate Drugs 0.000 claims description 4
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 2
- 150000002910 rare earth metals Chemical class 0.000 abstract description 8
- 238000005119 centrifugation Methods 0.000 abstract description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 10
- 239000002351 wastewater Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 230000008901 benefit Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 230000006870 function Effects 0.000 description 3
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000011953 bioanalysis Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229910021420 polycrystalline silicon Inorganic materials 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 238000011946 reduction process Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/30—Wastewater or sewage treatment systems using renewable energies
- Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of preparation method of use in waste water treatment denitrification microbial inoculum, belong to stain disease biologic treating technique field.Specifically preparation method is：(1) activated sludge with Denitrification is seeded in bioreactor.(2) under anoxia condition, maintain bioreactor temperature to be 20～35 DEG C, denitrifying bacterium culture medium is added in bioreactor in full-automatic continuum micromeehanics mode.(3) measure the utilization rate to organic carbon source for the above-mentioned reactor, according to testing result, graduation adds the rare earth of different amounts, to lift the nitrogen removal performance of the denitrification microbial inoculum finally preparing.(4) when above-mentioned reactor reaches 85% to the utilization rate of organic carbon source, the part culture fluid in reactor is taken out, concentration bacteria suspension is made in centrifugation.(5) to concentrating addition protective agent in bacteria suspension, uniformly drying is sprayed on the carrier of sterilizing, and hermetically storing makes denitrification microbial inoculum.The breeding activity function of the denitrification denitrogenation microbial inoculum of present invention preparation is all preferable, and the denitrification rate of microbial inoculum is very fast, more than 1.0mgNO3 ‑‑N/(mg VSS·d).Meanwhile, this method environmental protection, it is to avoid secondary pollution.
Description
Technical field
A kind of the present invention relates to stain disease biologic treating technique field, more particularly, it relates to use in waste water treatment denitrification
The preparation method of denitrogenation microbial inoculum.
Background technology
Denitrification denitrogenation technology is the relatively conventional a kind of technology in denitrogenation of waste water field in recent years, mainly uses microorganism
Denitrification realize the removal of the pollutant such as nitrate nitrogen in waste water, nitrite nitrogen, presently, there are that denitrification rate is relatively low, place
The problems such as reason efficiency is poor.For the problems referred to above, during denitrification denitrogenation, generally dirty by reduction process load, increase
Water retention time, the different Organic substances of selection improve denitrification treatment effect as measures such as electron donor, lifting sludge settlings,
Thus ensureing that water outlet can reach discharging standards, but the treatment effect of these measures is very unstable, and all can increase
Operational management and capital cost, produce economic pressures.For solving this difficult problem, many researcheres are thought, are obtained by screening
Obtaining denitrifying bacterium or the method for gathering denitrifying flora is to solve the optimal path of this problem.Therefore, prepare denitrifying bacterium
Agent becomes the study hotspot in denitrification denitrogenation technology.
At present, the research of denitrification microbial inoculum focuses primarily upon the preparation of aerobic denitrification microbial inoculum and application aspect (as China is special
Sharp application number 201410179355.1, the applying date is on April 30th, 2014, and invention and created name is that a kind of lake protection that strengthens takes off
The aerobic denitrification microbial inoculum preparation of nitrogen function and apply), (such as Chinese Patent Application No. 201310054249.6, the applying date is
On 2 20th, 2013, invention and created name was a kind of aerobic denitrification microbial inoculum and its preparation and application).But to anoxia condition
Under, the research of denitrification microbial inoculum is relatively fewer, and most of Denitrification is all carried out under anoxic conditions, so this
Bright more above-mentioned practical applicability is higher, usage range is wider.
Rare earth mainly includes two aspects to the mechanism of action of microorganism, is with microbial cell film as target organ first,
Act on biomacromolecules such as protein, enzymes, thus affecting some biological functions and the activity of cell.Current result of study is own
Find that rare earth can act on transport protein or phospholipid on cell membrane, improve the permeability of film.Secondly, the active change of rare earth element
Learn property and strong complexing power determines the scavenger being likely to become harmful free radicals in microbial body under certain condition.Have
Person it has been investigated that, rare earth can promote activity of anaerobic sludge, but promote the denitrification denitrogenation speed of denitrification microbial inoculum using rare earth
The method of rate has no report.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of preparation method of denitrification flora, prepared denitrification microbial inoculum
Strong adaptability, denitrification rate are high, have the function of once throwing bacterium life-time service.Just need not persistently add bacterium after system stability
Agent, greatlys save the operating cost of wastewater treatment, improves the convenience of wastewater treatment operation.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of preparation method of use in waste water treatment denitrification microbial inoculum is it is characterised in that described compound denitrification denitrogenation microbial inoculum
Obtained by activated sludge process acclimating, in acclimating incubation, the additional carbon of denitrification flora is lemon
Lemon acid sodium, adds compound rare-earth simultaneously in denitrifying bacterium culture medium and accelerates denitrification process, concrete preparation method is：
(1) activated sludge with Denitrification is seeded in bioreactor, needs before inoculation to determine seed sludge
Denitrification rate be not less than 0.55mgNO3 --N/(mg VSS·d)；
(2), under anoxia condition, bioreactor temperature is maintained to be 20～35 DEG C, in full-automatic continuum micromeehanics mode to biology
Add denitrifying bacterium culture medium, the composition of denitrifying bacterium culture medium is in reactor：KNO3For 1.0～2.0g/L, K2HPO4For
0.3～0.5g/L, KH2PO4For 0.5～1.0g/L, MgSO4·7H2O is 0.2～0.6g/L, CaCl2·2H2O be 0.02～
0.07g/L, trace element solution is 0.5～1.0ml/L；After 24h, improve K in denitrifying bacterium culture medium2HPO4、KH2PO4And
The concentration of trace element solution, wherein K2HPO4Concentration range is 0.2～2.0g/L, KH2PO4Concentration range is 0.5g～2.5g/
L, trace element solution concentration is 1.0～3.0ml/L；
(3) under conditions of maintaining bioreactor temperature to be 20～35 DEG C, add organic carbon to denitrifying bacterium culture medium
Source sodium citrate (Na3C6H5O7·2H2O) so as to concentration in the medium is 4.0～5.5g/L；
(4) measure the utilization rate to organic carbon source for the above-mentioned reactor, when above-mentioned reactor is low to the utilization rate of organic carbon source
When 70%, add at least one nitric acid rare earth in reactor, the addition of nitric acid rare earth be add 4.0 by every gram of VSS～
5.0 μ g nitric acid rare earths；Whne above-mentioned reactor to the utilization rate of organic carbon source 70%～85% when, continue to add in reactor
At least one nitric acid rare earth, the addition of nitric acid rare earth is to add 2.0～3.0 μ g nitric acid rare earths by every gram of VSS；Treat above-mentioned reaction
Device, to when the utilization rate of organic carbon source is higher than 85%, then no longer adds nitric acid rare earth in reactor；
(5) when above-mentioned reactor reaches 85% to the utilization rate of organic carbon source, the part culture fluid in reactor is taken
Go out, concentration bacteria suspension is made in centrifugation；
(6) to concentrating addition protective agent in bacteria suspension, uniformly drying is sprayed on the carrier of sterilizing, hermetically storing.
Further, in such scheme, the pH scope of the described denitrifying bacterium culture medium of step (2) is 6.5～7.5.
Further, in such scheme, the consisting of of step (2) described trace element solution：CuSO4·5H2O is
0.10～0.30g/L；FeCl3·6H2O is 1.0～2.0g/L；MnCl2·4H2O is 0.10～0.15g/L；ZnSO4·7H2O is
0.10～0.15g/L；Na2MoO4·2H2O is 0.02～0.06g/L；H3BO3For 0.10～0.15g/L；KI be 0.01～
0.02g/L；CoCl2·6H2O is 0.10～0.15g/L.
Further, in such scheme, the nitric acid rare earth being added twice in above step (4) all can be selected for Lanthanum (III) nitrate
(La(NO3)3·6H2O), cerous nitrate (Ce (NO3)3·6H2O), praseodymium nitrate H12N3O15Pr, neodymium nitrate (Nd (NO3)3·6H2In O
At least one.
Further, in such scheme, in denitrification process, the temperature of reactor should be maintained at 20～35 DEG C, pH scope
For 6.5～7.5.
Further, in such scheme, in step (5), the part culture fluid in reactor is taken out, centrifugation system
Become to concentrate bacteria suspension, its centrifugal condition is：8000～9500r/min, 8～10min, 25～30 DEG C.
Further, in such scheme, the described protective agent of step (6) is glycine is 1 with glycerol in mass ratio:2～3
Mixture, protectant addition is 2.0～3.5 times of bacteria suspension.
Further, in such scheme, the carrier described in step (6) is potassium carbonate, calcium alginate, chitin, Linesless charcoal
Black, magnesium stearate by weight 0.2::3:1:2:0.5 mixes, and their particle diameter is 80-100 mesh.This carrier can be protected
Card is used for the good advantage of tack in biologic packing material in microbial inoculum, also has wide material sources, with low cost, easy to use, preservation term
The advantages of length, preparation method are simple.
Compared with prior art, beneficial effects of the present invention are embodied in：
(1) microbial inoculum of the present invention can fast breeding, thus reducing initial cost.Prepared compound denitrification microbial inoculum adapts to
Property strong, there is the function of once throwing bacterium life-time service, just need not persistently add microbial inoculum after system stability, greatly save waste water
The operating cost processing, improves the convenience of wastewater treatment operation.And this microbial inoculum is widely used in nitrate nitrogen under anoxia condition
Removal, environmentally friendly, have a extensive future, there is higher social benefit.
(2) present invention is on the basis of bioanalysises, by adding the lifting to denitrification rate for the chemical agent rare earth realization,
Take full advantage of the advantage of bioanalysises and chemical method so that the denitrification rate of denitrification microbial inoculum is in 1.0mgNO3 --N/(mg
VSS d) more than, it is achieved thereby that the lifting to denitrification microbial inoculum nitrogen removal performance.
It is embodied as case
For further appreciating that present disclosure, with reference to embodiment, the invention will be further described.
Embodiment 1
The preparation of polycrystalline silicon solar cell nitrate nitrogen use in waste water treatment denitrification microbial inoculum
(1) activated sludge with Denitrification is seeded in bioreactor, needs before inoculation to determine seed sludge
Denitrification rate be not less than 0.55mgNO3 --N/(mg VSS·d)；
(2), under anoxia condition, bioreactor temperature is maintained to be 20 DEG C, in full-automatic continuum micromeehanics mode to biological respinse
Add denitrifying bacterium culture medium, the composition of denitrifying bacterium culture medium is in device：KNO3For 1.0g/L, K2HPO4For 0.3g/L,
KH2PO4For 0.5g/L, MgSO4·7H2O is 0.2g/L, CaCl2·2H2O is 0.02g/L, and trace element solution is 0.5ml/L,
The pH scope of denitrifying bacterium culture medium is 6.5；After 24h, improve K in denitrifying bacterium culture medium2HPO4、KH2PO4And micro unit
The concentration of plain solution, wherein K2HPO4Concentration range is 0.2g/L, KH2PO4Concentration range is 0.5g/L, and trace element solution is dense
Spend for 1.0ml/L；The consisting of of trace element solution：CuSO4·5H2O is 0.10g/L；FeCl3·6H2O is 1.0g/L；
MnCl2·4H2O is 0.10g/L；ZnSO4·7H2O is 0.10g/L；Na2MoO4·2H2O is 0.02g/L；H3BO3For 0.10g/L；
KI is 0.01g/L；CoCl2·6H2O is 0.10g/L.
(3) under conditions of maintaining bioreactor temperature to be 20 DEG C, add organic carbon source lemon to denitrifying bacterium culture medium
Lemon acid sodium (Na3C6H5O7·2H2O) so as to concentration in the medium is 4.0g/L；
(4) measure the utilization rate to organic carbon source for the above-mentioned reactor, when above-mentioned reactor is low to the utilization rate of organic carbon source
When 70%, add at least one nitric acid rare earth in reactor, the addition of nitric acid rare earth is to add 4.0 μ g by every gram of VSS
Nitric acid rare earth；Whne above-mentioned reactor to the utilization rate of organic carbon source 70% when, continue to add at least one nitre in reactor
Acid rare earth, the addition of nitric acid rare earth is to add 2.0 μ g nitric acid rare earths by every gram of VSS；Treat above-mentioned reactor to organic carbon source
When utilization rate is higher than 85%, in reactor, then no longer add nitric acid rare earth；Below the nitric acid rare earth being added twice all can be selected for
Lanthanum (III) nitrate (La (NO3)3·6H2O), cerous nitrate (Ce (NO3)3·6H2O), praseodymium nitrate H12N3O15Pr, neodymium nitrate (Nd (NO3)3·
6H2At least one in O, in denitrification process, the temperature of reactor should be maintained at 20 DEG C, and pH scope is 6.5.
(5) when above-mentioned reactor reaches 85% to the utilization rate of organic carbon source, the part culture fluid in reactor is taken
Go out, concentration bacteria suspension is made in centrifugation, its centrifugal condition is：8000r/min, 8min, 25 DEG C；
(6) to concentrating addition protective agent in bacteria suspension, (protective agent is glycine is 1 with glycerol in mass ratio:2 mixing
Thing, protectant addition is 2.0 times of bacteria suspension), uniformly drying is sprayed on carrier, carrier is potassium carbonate, calcium alginate,
Chitin, white carbon, magnesium stearate by weight 0.2::3:1:2:0.5 mixes, and their particle diameter is 80 mesh, sealing
Storage；Microbial inoculum hermetically storing took out after 15 days, and the ratio throwing in 1.2 kilograms of denitrification microbial inoculum according to 1 cubic metre of waste water is past
Throw in waste water.
(7) after adding the compound denitrification microbial inoculum 36h of the present invention, the anti-nitre of polycrystalline silicon solar cell nitrate nitrogen Waste Water Treatment
Changing speed substantially increases.After input, its denitrification rate is 1.05mgNO3 -- N/ (mg VSS d), improves than before input
0.45mgNO3 --N/mg VSS·d.The water treatment effect adding denitrification microbial inoculum is substantially better than the situation not throwing bacterium, and nitrate nitrogen is gone
Except rate brings up to 85.06% by 56.03% before, water outlet nitrate nitrogen concentration is down to 10mg/L from 85mg/L.
Embodiment 2
Town sewage processes the preparation with denitrification microbial inoculum
(1) activated sludge with Denitrification is seeded in bioreactor, needs before inoculation to determine seed sludge
Denitrification rate be not less than 0.55mgNO3 --N/(mg VSS·d)；
(2), under anoxia condition, bioreactor temperature is maintained to be 27.5 DEG C, anti-to biology in full-automatic continuum micromeehanics mode
Answer interpolation denitrifying bacterium culture medium in device, the composition of denitrifying bacterium culture medium is：KNO3For 1.5g/L, K2HPO4For 0.4g/L,
KH2PO4For 0.75g/L, MgSO4·7H2O is 0.4g/L, CaCl2·2H2O is 0.045g/L, and trace element solution is 0.75ml/
L, the pH scope of denitrifying bacterium culture medium is 7.0；After 24h, improve K in denitrifying bacterium culture medium2HPO4、KH2PO4And it is micro
The concentration of Element Solution, wherein K2HPO4Concentration range is 1.1g/L, KH2PO4Concentration range is 1.5g/L, trace element solution
Concentration is 2.0ml/L；The consisting of of trace element solution：CuSO4·5H2O is 0.120g/L；FeCl3·6H2O is 1.5g/L；
MnCl2·4H2O is 0.125g/L；ZnSO4·7H2O is 0.125g/L；Na2MoO4·2H2O is 0.04g/L；H3BO3For
0.125g/L；KI is 0.015g/L；CoCl2·6H2O is 0.125g/L.
(3) under conditions of maintaining bioreactor temperature to be 27.5 DEG C, add organic carbon source to denitrifying bacterium culture medium
Sodium citrate (Na3C6H5O7·2H2O) so as to concentration in the medium is 4.75g/L；
(4) measure the utilization rate to organic carbon source for the above-mentioned reactor, when above-mentioned reactor is low to the utilization rate of organic carbon source
When 70%, add at least one nitric acid rare earth in reactor, the addition of nitric acid rare earth is to add 4.5 μ g by every gram of VSS
Nitric acid rare earth；Whne above-mentioned reactor to the utilization rate of organic carbon source 77.5% when, continue to add in reactor at least one
Nitric acid rare earth, the addition of nitric acid rare earth is to add 2.5 μ g nitric acid rare earths by every gram of VSS；Treat above-mentioned reactor to organic carbon source
Utilization rate be higher than 85% when, in reactor then no longer add nitric acid rare earth；Below the nitric acid rare earth being added twice is all optional
With Lanthanum (III) nitrate (La (NO3)3·6H2O), cerous nitrate (Ce (NO3)3·6H2O), praseodymium nitrate H12N3O15Pr, neodymium nitrate (Nd
(NO3)3·6H2At least one in O, in denitrification process, the temperature of reactor should be maintained at 27.5 DEG C, and pH scope is 7.0.
(5) when above-mentioned reactor reaches 85% to the utilization rate of organic carbon source, the part culture fluid in reactor is taken
Go out, concentration bacteria suspension is made in centrifugation, its centrifugal condition is：8750r/min, 9min, 27.5 DEG C；
(6) to concentrating addition protective agent in bacteria suspension, (protective agent is glycine is 1 with glycerol in mass ratio:2.5 mixing
Thing, protectant addition is 2.75 times of bacteria suspension), uniformly drying is sprayed on carrier, and carrier is potassium carbonate, alginic acid
Calcium, chitin, white carbon, magnesium stearate by weight 0.2::3:1:2:0.5 mixes, and their particle diameter is 90 mesh, close
Envelope storage；Microbial inoculum hermetically storing took out after 10 days, threw in the ratio of 1.0 kilograms of denitrification microbial inoculum according to 1 cubic metre of waste water
Throw in toward in waste water.
(7), after adding the compound denitrification microbial inoculum 36h of the present invention, the denitrification rate of town sewage processing system substantially increases
Plus.After input, its denitrification rate is 1.34mgNO3 -- N/ (mg VSS d), improves 0.71mgNO than before input3 --N/
(mg VSS·d).The water treatment effect adding denitrification microbial inoculum is substantially better than the situation not throwing bacterium, and nitrate nitrogen clearance is by before
60.25% bring up to 89.77%, water outlet nitrate nitrogen concentration is down to 12mg/L from 105mg/L.
Embodiment 3
The cultivating wastewater purification preparation of denitrification microbial inoculum
(1) activated sludge with Denitrification is seeded in bioreactor, needs before inoculation to determine seed sludge
Denitrification rate be not less than 0.55mgNO3 --N/(mg VSS·d)；
(2), under anoxia condition, bioreactor temperature is maintained to be 35 DEG C, in full-automatic continuum micromeehanics mode to biological respinse
Add denitrifying bacterium culture medium, the composition of denitrifying bacterium culture medium is in device：KNO3For 2.0g/L, K2HPO4For 0.5g/L,
KH2PO4For 1.0g/L, MgSO4·7H2O is 0.6g/L, CaCl2·2H2O is 0.07g/L, and trace element solution is 1.0ml/L,
The pH scope of denitrifying bacterium culture medium is 7.5；After 24h, improve K in denitrifying bacterium culture medium2HPO4、KH2PO4And micro unit
The concentration of plain solution, wherein K2HPO4Concentration range is 2.0g/L, KH2PO4Concentration range is 2.5g/L, and trace element solution is dense
Spend for 3.0ml/L；The consisting of of trace element solution：CuSO4·5H2O is 0.30g/L；FeCl3·6H2O is 2.0g/L；
MnCl2·4H2O is 0.15g/L；ZnSO4·7H2O is 0.15g/L；Na2MoO4·2H2O is 0.06g/L；H3BO3For 0.15g/L；
KI is 0.02g/L；CoCl2·6H2O is 0.15g/L.
(3) under conditions of maintaining bioreactor temperature to be 35 DEG C, add organic carbon source lemon to denitrifying bacterium culture medium
Lemon acid sodium (Na3C6H5O7·2H2O) so as to concentration in the medium is 5.5g/L；
(4) measure the utilization rate to organic carbon source for the above-mentioned reactor, when above-mentioned reactor is low to the utilization rate of organic carbon source
When 70%, add at least one nitric acid rare earth in reactor, the addition of nitric acid rare earth is to add 5.0 μ g by every gram of VSS
Nitric acid rare earth；Whne above-mentioned reactor to the utilization rate of organic carbon source 85% when, continue to add at least one nitre in reactor
Acid rare earth, the addition of nitric acid rare earth is to add 3.0 μ g nitric acid rare earths by every gram of VSS；Treat above-mentioned reactor to organic carbon source
When utilization rate is higher than 85%, in reactor, then no longer add nitric acid rare earth；Below the nitric acid rare earth being added twice all can be selected for
Lanthanum (III) nitrate (La (NO3)3·6H2O), cerous nitrate (Ce (NO3)3·6H2O), praseodymium nitrate H12N3O15Pr, neodymium nitrate (Nd (NO3)3·
6H2At least one in O, in denitrification process, the temperature of reactor should be maintained at 35 DEG C, and pH scope is 7.5.
(5) when above-mentioned reactor reaches 85% to the utilization rate of organic carbon source, the part culture fluid in reactor is taken
Go out, concentration bacteria suspension is made in centrifugation, its centrifugal condition is：9500r/min, 10min, 30 DEG C；
(6) to concentrating addition protective agent in bacteria suspension, (protective agent is glycine is 1 with glycerol in mass ratio:3 mixing
Thing, protectant addition is 3.5 times of bacteria suspension), uniformly drying is sprayed on carrier, carrier is potassium carbonate, calcium alginate,
Chitin, white carbon, magnesium stearate by weight 0.2::3:1:2:0.5 mixes, and their particle diameter is 100 mesh, sealing
Storage；Microbial inoculum hermetically storing took out after 15 days, and the ratio throwing in 1.5 kilograms of denitrification microbial inoculum according to 1 cubic metre of waste water is past
Throw in waste water.
(7), after adding the compound denitrification microbial inoculum 36h of the present invention, the denitrification rate of town sewage processing system substantially increases
Plus.After input, its denitrification rate is 1.44mgNO3 -- N/ (mg VSS d), improves 0.88mgNO than before input3 --N/
(mg VSS·d).The water treatment effect adding denitrification microbial inoculum is substantially better than the situation not throwing bacterium, and nitrate nitrogen clearance is by before
62.17% bring up to 89.45%, water outlet nitrate nitrogen concentration is down to 8mg/L from 96mg/L.
What deserves to be explained is, to those skilled in the art, under present inventive concept and specific embodiment enlighten, energy
Enough some deformation directly derived or associate from the disclosure of invention and general knowledge, it will be recognized by those of ordinary skill in the art that
The replacement of in additive method, or prior art conventional known technology may also be employed, and the mutually different combination between feature etc.
Unsubstantiality change, equally can be employed, can realize the present invention description function and effect, no longer one by one citing launch
Describe in detail, belong to the scope of the present invention.
Claims (8)
1. a kind of preparation method of use in waste water treatment denitrification microbial inoculum is it is characterised in that described compound denitrification denitrogenation microbial inoculum is
Obtained by activated sludge process acclimating, in acclimating incubation, the additional carbon of denitrification flora is Fructus Citri Limoniae
Sour sodium, adds compound rare-earth simultaneously in denitrifying bacterium culture medium and accelerates denitrification process, concrete preparation method is：
(1) activated sludge with Denitrification is seeded in bioreactor, needs before inoculation to determine the anti-of seed sludge
Rate of nitrification is not less than 0.55mgNO3 --N/(mg VSS·d)；
(2), under anoxia condition, bioreactor temperature is maintained to be 20～35 DEG C, in full-automatic continuum micromeehanics mode to biological respinse
Add denitrifying bacterium culture medium, the composition of denitrifying bacterium culture medium is in device：KNO3For 1.0～2.0g/L, K2HPO4For 0.3～
0.5g/L, KH2PO4For 0.5～1.0g/L, MgSO4·7H2O is 0.2～0.6g/L, CaCl2·2H2O is 0.02～0.07g/L,
Trace element solution is 0.5～1.0ml/L；After 24h, improve K in denitrifying bacterium culture medium2HPO4、KH2PO4And trace element
The concentration of solution, wherein K2HPO4Concentration range is 0.2～2.0g/L, KH2PO4Concentration range is 0.5g～2.5g/L, micro unit
Plain solution concentration is 1.0～3.0ml/L；
(3) under conditions of maintaining bioreactor temperature to be 20～35 DEG C, add organic carbon source lemon to denitrifying bacterium culture medium
Lemon acid sodium (Na3C6H5O7·2H2O) so as to concentration in the medium is 4.0～5.5g/L；
(4) measure the utilization rate to organic carbon source for the above-mentioned reactor, when above-mentioned reactor is less than to the utilization rate of organic carbon source
When 70%, add at least one nitric acid rare earth in reactor, the addition of nitric acid rare earth is to add 4.0～5.0 by every gram of VSS
μ g nitric acid rare earth；Whne above-mentioned reactor to the utilization rate of organic carbon source 70%～85% when, continue to add in reactor to
A kind of few nitric acid rare earth, the addition of nitric acid rare earth is to add 2.0～3.0 μ g nitric acid rare earths by every gram of VSS；Treat above-mentioned reactor
To when the utilization rate of organic carbon source is higher than 85%, in reactor, then no longer add nitric acid rare earth；
(5) when above-mentioned reactor reaches 85% to the utilization rate of organic carbon source, the part culture fluid in reactor is taken out, from
The heart separates and makes concentration bacteria suspension.
(6) to concentrating addition protective agent in bacteria suspension, uniformly drying is sprayed on the carrier of sterilizing, hermetically storing.
2. as claimed in claim 1 a kind of preparation method of use in waste water treatment denitrification microbial inoculum it is characterised in that step (2)
The pH scope of described denitrifying bacterium culture medium is 6.5～7.5.
3. a kind of preparation method of use in waste water treatment denitrification microbial inoculum as claimed in claim 2, the described trace element of step (2)
The consisting of of solution：CuSO4·5H2O is 0.10～0.30g/L；FeCl3·6H2O is 1.0～2.0g/L；MnCl2·4H2O is
0.10～0.15g/L；ZnSO4·7H2O is 0.10～0.15g/L；Na2MoO4·2H2O is 0.02～0.06g/L；H3BO3For
0.10～0.15g/L；KI is 0.01～0.02g/L；CoCl2·6H2O is 0.10～0.15g/L.
4. as claimed in claim 1 a kind of preparation method of use in waste water treatment denitrification microbial inoculum it is characterised in that described
The described nitric acid rare earth of step (4) can be selected for Lanthanum (III) nitrate (La (NO3)3·6H2O), cerous nitrate (Ce (NO3)3·6H2O), praseodymium nitrate
H12N3O15Pr, neodymium nitrate (Nd (NO3)3·6H2At least one in O.
5. as claimed in claim 1 a kind of preparation method of use in waste water treatment denitrification microbial inoculum it is characterised in that denitrification mistake
In journey, the temperature of reactor should be maintained at 20～35 DEG C, and pH scope is 6.5～7.5.
6. as claimed in claim 1 a kind of preparation method of use in waste water treatment denitrification microbial inoculum it is characterised in that step (5)
Described centrifugal condition is：8000～9500r/min, 8～10min, 25～30 DEG C.
7. as claimed in claim 1 a kind of preparation method of use in waste water treatment denitrification microbial inoculum it is characterised in that step (6)
Described protective agent is that glycine presses 1 with glycerol:The mixture of the ratio composition of (2～3), protectant addition is bacteria suspension
2.0～3.5 times.
8. as claimed in claim 1 a kind of preparation method of use in waste water treatment denitrification microbial inoculum it is characterised in that step (6)
Described carrier is potassium carbonate, calcium alginate, chitin, white carbon, the mixture of magnesium stearate, and their particle diameter is 80-
100 mesh.
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| CN113104985A (en) * | 2021-04-13 | 2021-07-13 | 哈尔滨工业大学 | Method for assisting in improving sewage treatment efficiency of bacterial-algae symbiotic system by using rare earth |
| CN117023831A (en) * | 2023-03-13 | 2023-11-10 | 湖北省协诚交通环保有限公司 | Method for rapidly realizing low-carbon-consumption synchronous nitrification and denitrification high-efficiency denitrification |
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| CN110550734A (en) * | 2019-08-30 | 2019-12-10 | 天津大学 | Biological promoter and application thereof |
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