CN113088413B - Preparation method of handmade soap capable of removing bacterial biofilm - Google Patents
Preparation method of handmade soap capable of removing bacterial biofilm Download PDFInfo
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- CN113088413B CN113088413B CN202110322225.9A CN202110322225A CN113088413B CN 113088413 B CN113088413 B CN 113088413B CN 202110322225 A CN202110322225 A CN 202110322225A CN 113088413 B CN113088413 B CN 113088413B
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- 239000000344 soap Substances 0.000 title claims abstract description 51
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title abstract description 6
- 239000010495 camellia oil Substances 0.000 claims abstract description 16
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 claims abstract description 15
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 claims abstract description 15
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 claims abstract description 15
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 claims abstract description 15
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 claims abstract description 15
- 229940074393 chlorogenic acid Drugs 0.000 claims abstract description 15
- 235000001368 chlorogenic acid Nutrition 0.000 claims abstract description 15
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 claims abstract description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 81
- 238000003756 stirring Methods 0.000 claims description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 239000007788 liquid Substances 0.000 claims description 23
- 239000003513 alkali Substances 0.000 claims description 18
- 239000003921 oil Substances 0.000 claims description 18
- 235000019198 oils Nutrition 0.000 claims description 18
- 235000019482 Palm oil Nutrition 0.000 claims description 12
- 239000002540 palm oil Substances 0.000 claims description 12
- 239000008149 soap solution Substances 0.000 claims description 11
- 235000019864 coconut oil Nutrition 0.000 claims description 10
- 239000003240 coconut oil Substances 0.000 claims description 10
- 238000007605 air drying Methods 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 9
- 235000011837 pasties Nutrition 0.000 claims description 9
- 239000008399 tap water Substances 0.000 claims description 9
- 235000020679 tap water Nutrition 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 3
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 6
- 206010059866 Drug resistance Diseases 0.000 description 4
- 239000002585 base Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000036541 health Effects 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003235 crystal violet staining Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D9/00—Compositions of detergents based essentially on soap
- C11D9/02—Compositions of detergents based essentially on soap on alkali or ammonium soaps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
- A61K31/216—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acids having aromatic rings, e.g. benactizyne, clofibrate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D13/00—Making of soap or soap solutions in general; Apparatus therefor
- C11D13/14—Shaping
- C11D13/16—Shaping in moulds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/382—Vegetable products, e.g. soya meal, wood flour, sawdust
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D9/00—Compositions of detergents based essentially on soap
- C11D9/005—Synthetic soaps
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D9/00—Compositions of detergents based essentially on soap
- C11D9/04—Compositions of detergents based essentially on soap containing compounding ingredients other than soaps
- C11D9/22—Organic compounds, e.g. vitamins
- C11D9/26—Organic compounds, e.g. vitamins containing oxygen
Abstract
The invention discloses a preparation method of handmade soap capable of obviously removing bacterial biofilm. The invention adopts natural chlorogenic acid and tea seed oil as main active ingredients, and the handmade soap capable of obviously removing bacterial biofilms is prepared by matching, so as to provide a new choice for inhibiting harmful bacteria and reducing the harm of the harmful bacterial biofilms.
Description
Technical Field
The invention relates to a preparation method of handmade soap capable of removing bacterial biofilm, and belongs to the technical field of handmade soap.
Background
With the improvement of the living standard of people, the green and environment-friendly health concept is increasingly deep in the mind, and the old handmade soap is returned to the field of vision of people. Because of the inexpensive price of handmade soaps, bad merchants have introduced them to imitate and impersonate them. Some of the soap base is crisp and takes the "handmade" two words as an article, and based on cheap soap base, and chemical synthesis reagents such as pigment, essence, preservative, antioxidant and the like are added to make the soap base soap with bright color and fragrant smell, and finally the soap base soap is marketed in the form of "handmade soap", so that the benefit of consumers is seriously infringed, and the handmade soap market is disturbed.
At present, the drug resistance problem of various pathogenic bacteria is more and more serious, the health of people is seriously threatened, and especially the bacterial biofilm (biofilm) has stronger drug resistance than planktonic bacteria, and the harm is more serious. In recent years, the related activity of natural compounds attracts attention, and the natural compounds are not easy to form drug resistance, have small side effects and have wide prospects. The natural active ingredients are adopted to remove the bacterial biofilm, so that the drug resistance of bacteria and adverse reactions of medicines can be avoided.
The method aims to provide a preparation method of the traditional handmade soap, and the preparation method of the handmade soap has the function of removing harmful bacterial biomembrane, so that the harm of the bacterial biomembrane is eliminated, and the health of people is protected.
Disclosure of Invention
(1) Taking 10-20 parts of tap water, taking 3-20 parts of sodium hydroxide, adding sodium hydroxide into the water while stirring, stirring until the sodium hydroxide is completely dissolved in the water, and naturally cooling to 36-46 ℃ to obtain alkali liquor;
(2) Taking 3-20 parts of coconut oil and 5-60 parts of palm oil, placing the palm oil in a reaction kettle, and heating the palm oil in a water bath to 36-46 ℃ to obtain mixed oil;
(3) Slowly adding the alkali liquor obtained in the step (1) into the mixed oil liquid obtained in the step (2) under the condition of stirring, controlling the temperature at 40-60 ℃, continuously stirring until the mixed liquid is pasty, adding 0.1-10 parts of tea seed oil, adding 0.5-10 parts of chlorogenic acid, and uniformly stirring to obtain soap liquid;
(4) Pouring the soap solution obtained in the step (3) into a mold, placing the mold in a room for 2-5d, demolding, and air-drying for 1-16d to obtain the handmade soap capable of obviously removing bacterial biofilm.
The invention has the beneficial effects that:
The inhibitor provided by the invention adopts chlorogenic acid with high safety as a main active ingredient, and camellia oil as an auxiliary ingredient, so that the chlorogenic acid and the camellia oil can be cooperatively matched to influence the physiological function of a bacterial biological film, and the biological film removing effect of the handmade soap is further enhanced.
The handmade soap disclosed by the invention is prepared from natural raw materials, so that the safety of the handmade soap is obviously improved, the skin is obviously protected, and the side effect is obviously reduced.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for better illustration of the invention, and should not be construed as limiting the invention.
Example 1
Taking 13 parts of tap water and 5 parts of sodium hydroxide, adding sodium hydroxide into the water while stirring, stirring until the sodium hydroxide is completely dissolved in the water, and naturally cooling to 46 ℃ to obtain alkali liquor;
10 parts of coconut oil and 35 parts of palm oil are taken and placed in a reaction kettle, and water bath is carried out to heat to 43 ℃ to obtain mixed oil;
Slowly adding alkali liquor into the mixed oil under the condition of stirring, controlling the temperature at 55 ℃, continuously stirring until the mixed liquor is pasty, adding 3 parts of tea seed oil, adding 4 parts of chlorogenic acid, and uniformly stirring to obtain soap solution;
pouring soap liquid into a mold, placing the mold in a room for 3d, demolding, and air-drying for 7d to obtain the handmade soap capable of remarkably removing bacterial biofilm.
Example 2
Taking 11 parts of tap water and 5 parts of sodium hydroxide, adding sodium hydroxide into the water while stirring, stirring until the sodium hydroxide is completely dissolved in the water, and naturally cooling to 46 ℃ to obtain alkali liquor;
10 parts of coconut oil and 37 parts of palm oil are taken and placed in a reaction kettle, and water bath is carried out to heat the mixture to 46 ℃ to obtain mixed oil liquid;
Slowly adding alkali liquor into the mixed oil under the condition of stirring, controlling the temperature at 55 ℃, continuously stirring until the mixed liquor is pasty, adding 1 part of tea seed oil, adding 7 parts of chlorogenic acid, and uniformly stirring to obtain soap solution;
pouring soap liquid into a mold, placing the mold in a room for 3d, demolding, and air-drying for 8d to obtain the handmade soap capable of obviously removing bacterial biofilm.
Example 3
Taking 18 parts of tap water and 6 parts of sodium hydroxide, adding sodium hydroxide into the water while stirring, stirring until the sodium hydroxide is completely dissolved in the water, and naturally cooling to 40 ℃ to obtain alkali liquor;
taking 20 parts of coconut oil and 27 parts of palm oil, and placing the coconut oil and the palm oil into a reaction kettle, and heating the mixture to 46 ℃ in a water bath to obtain mixed oil liquid;
slowly adding alkali liquor into the mixed oil under stirring, controlling the temperature at 59 ℃, continuously stirring until the mixed liquor is pasty, adding 4 parts of tea seed oil, adding 5 parts of chlorogenic acid, and uniformly stirring to obtain soap solution;
Pouring soap liquid into a mold, placing the mold in a room for 2d, demolding, and air-drying for 10d to obtain the handmade soap capable of remarkably removing bacterial biofilm.
Example 4 cleaning action of handmade soap on bacterial biofilm
Experiment group 1:
Taking 11 parts of tap water and 5 parts of sodium hydroxide, adding sodium hydroxide into the water while stirring, stirring until the sodium hydroxide is completely dissolved in the water, and naturally cooling to 46 ℃ to obtain alkali liquor;
10 parts of coconut oil and 37 parts of palm oil are taken and placed in a reaction kettle, and water bath is carried out to heat the mixture to 46 ℃ to obtain mixed oil liquid;
Slowly adding alkali liquor into the mixed oil under the condition of stirring, controlling the temperature at 55 ℃, continuously stirring until the mixed liquor is pasty, adding 1 part of tea seed oil, adding 1 part of chlorogenic acid, and uniformly stirring to obtain soap solution;
pouring soap liquid into a mold, placing the mold in a room for 3d, demolding, and air-drying for 8d to obtain the handmade soap capable of obviously removing bacterial biofilm.
Experiment group 2:
Taking 11 parts of tap water and 5 parts of sodium hydroxide, adding sodium hydroxide into the water while stirring, stirring until the sodium hydroxide is completely dissolved in the water, and naturally cooling to 46 ℃ to obtain alkali liquor;
10 parts of coconut oil and 37 parts of palm oil are taken and placed in a reaction kettle, and water bath is carried out to heat the mixture to 46 ℃ to obtain mixed oil liquid;
slowly adding alkali liquor into the mixed oil under the condition of stirring, controlling the temperature at 55 ℃, continuously stirring until the mixed liquor is pasty, adding 1 part of tea seed oil, adding 0 part of chlorogenic acid, and uniformly stirring to obtain soap solution;
pouring soap liquid into a mold, placing the mold in a room for 3d, demolding, and air-drying for 8d to obtain the handmade soap capable of obviously removing bacterial biofilm.
Experiment group 3:
Taking 11 parts of tap water and 5 parts of sodium hydroxide, adding sodium hydroxide into the water while stirring, stirring until the sodium hydroxide is completely dissolved in the water, and naturally cooling to 46 ℃ to obtain alkali liquor;
10 parts of coconut oil and 37 parts of palm oil are taken and placed in a reaction kettle, and water bath is carried out to heat the mixture to 46 ℃ to obtain mixed oil liquid;
slowly adding alkali liquor into the mixed oil under the condition of stirring, controlling the temperature at 55 ℃, continuously stirring until the mixed liquor is pasty, adding 0 part of tea seed oil, adding 1 part of chlorogenic acid, and uniformly stirring to obtain soap solution;
pouring soap liquid into a mold, placing the mold in a room for 3d, demolding, and air-drying for 8d to obtain the handmade soap capable of obviously removing bacterial biofilm.
Experiment group 4:
Taking 11 parts of tap water and 5 parts of sodium hydroxide, adding sodium hydroxide into the water while stirring, stirring until the sodium hydroxide is completely dissolved in the water, and naturally cooling to 46 ℃ to obtain alkali liquor;
10 parts of coconut oil and 37 parts of palm oil are taken and placed in a reaction kettle, and water bath is carried out to heat the mixture to 46 ℃ to obtain mixed oil liquid;
slowly adding alkali liquor into the mixed oil under the condition of stirring, controlling the temperature at 55 ℃, continuously stirring until the mixed liquor is pasty, adding 0 part of tea seed oil, adding 0 part of chlorogenic acid, and uniformly stirring to obtain soap solution;
pouring soap liquid into a mold, placing the mold in a room for 3d, demolding, and air-drying for 8d to obtain the handmade soap capable of obviously removing bacterial biofilm.
Measurement method
The experiment of removing the biological film is carried out by referring to the common experiment method at home and abroad. Taking 2 g of handmade soap sample, grinding, adding 10mL of water, and uniformly mixing to prepare the handmade soap liquid for later use. Adding staphylococcus aureus suspension into a 96-well plate, culturing for 24 hours at 37 ℃, discarding plankton bacteria, and washing for 3 times by using phosphate buffer solution; then adding culture medium, adding handmade soap liquid, and culturing at 37 deg.C for 4 hr.
The biofilm was analyzed by crystal violet staining. After the constant temperature culture is completed, the plankton is discarded, washed for 3 times, and dried and fixed at 60 ℃. Then adding 200 mu L of crystal violet solution (w/v) into each hole, dyeing for 5min, washing, flushing out redundant crystal violet dyeing liquid, drying at 37 ℃, adding 200 mu L of 95% ethanol for dissolving, measuring an OD value at 595nm after 30min, and calculating the clearance of the biological film.
Biofilm clearance= (OD of sample without soap solution-OD of sample with soap solution)/OD of sample without soap solution
The specific results are shown in Table 1.
Table 1 results of removal of biofilm by handsoaps from different experimental groups
The measurement result shows that the handmade soap containing 1 part of tea seed oil and 1 part of chlorogenic acid prepared in the example 1 can obviously remove bacterial biomembrane, and the removal rate reaches 93%. In the case of the simple handmade soap containing 1 part of tea seed oil, the handmade soap does not show the scavenging ability under the same use concentration. For the simple handmade soap containing 1 part of chlorogenic acid, the biological film removing capacity of the handmade soap is only 36% under the same use concentration, and for the handmade soap without the tea seed oil and the peach separating component, the biological film removing capacity is not shown under the same use concentration, which shows that: chlorogenic acid and tea seed oil can have synergistic effect, so that bacterial biofilm can be remarkably removed.
Claims (1)
1. A method for preparing handmade soap capable of remarkably removing bacterial biofilm, which comprises the following steps:
(1) Taking 11 parts of tap water and 5 parts of sodium hydroxide, adding sodium hydroxide into the water while stirring, stirring until the sodium hydroxide is completely dissolved in the water, and naturally cooling to 46 ℃ to obtain alkali liquor;
(2) 10 parts of coconut oil and 37 parts of palm oil are taken and placed in a reaction kettle, and water bath is carried out to heat the mixture to 46 ℃ to obtain mixed oil liquid;
(3) Slowly adding the alkali liquor obtained in the step (1) into the mixed oil liquid obtained in the step (2) under the condition of stirring, controlling the temperature at 55 ℃, continuously stirring until the mixed liquid is pasty, adding 1 part of tea seed oil, adding 1 part of chlorogenic acid, and uniformly stirring to obtain soap liquid;
(4) Pouring the soap solution obtained in the step (3) into a mold, placing the mold in a room for 3d, demolding, and air-drying for 8d to obtain the handmade soap capable of removing bacterial biofilm.
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| CN202110322225.9A CN113088413B (en) | 2021-03-25 | 2021-03-25 | Preparation method of handmade soap capable of removing bacterial biofilm |
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| CN202110322225.9A CN113088413B (en) | 2021-03-25 | 2021-03-25 | Preparation method of handmade soap capable of removing bacterial biofilm |
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| CN113088413B true CN113088413B (en) | 2024-04-19 |
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| CN115873672A (en) * | 2022-07-29 | 2023-03-31 | 江南大学 | Bacterial biofilm scavenger and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467978A (en) * | 2019-09-11 | 2019-11-19 | 福建师范大学泉港石化研究院 | A kind of whitening antibacterial grifolin soap and preparation method thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467978A (en) * | 2019-09-11 | 2019-11-19 | 福建师范大学泉港石化研究院 | A kind of whitening antibacterial grifolin soap and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| Developing natural products as potential anti‑biofilm agents;Lan Lu etal.;《Chinese Medicine》;20190320;第14卷;第1-17页 * |
| 绿原酸类物质在畜禽生产中的应用及研究进展;陈芳等;《湖北农业科学》;第59卷(第21期);第10-13，16页 * |
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