CN113080312A - Small peptide-rich bacterium-enzyme synergistic fermented chicken sausage and preparation method thereof - Google Patents
Small peptide-rich bacterium-enzyme synergistic fermented chicken sausage and preparation method thereof Download PDFInfo
- Publication number
- CN113080312A CN113080312A CN202110488610.0A CN202110488610A CN113080312A CN 113080312 A CN113080312 A CN 113080312A CN 202110488610 A CN202110488610 A CN 202110488610A CN 113080312 A CN113080312 A CN 113080312A
- Authority
- CN
- China
- Prior art keywords
- chicken
- preparing
- material according
- chicken intestine
- fermented feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 68
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 8
- 235000013580 sausages Nutrition 0.000 title abstract description 3
- 230000002195 synergetic effect Effects 0.000 title description 3
- 210000000936 intestine Anatomy 0.000 claims abstract description 61
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 238000000034 method Methods 0.000 claims abstract description 30
- 238000011081 inoculation Methods 0.000 claims abstract description 29
- 239000002994 raw material Substances 0.000 claims abstract description 27
- 239000000463 material Substances 0.000 claims abstract description 24
- 102000004190 Enzymes Human genes 0.000 claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 claims abstract description 17
- 239000004365 Protease Substances 0.000 claims abstract description 17
- 150000001875 compounds Chemical class 0.000 claims abstract description 11
- 230000000968 intestinal effect Effects 0.000 claims abstract description 11
- 238000013329 compounding Methods 0.000 claims abstract description 10
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 4
- 235000019621 digestibility Nutrition 0.000 claims abstract description 4
- 238000011049 filling Methods 0.000 claims abstract description 4
- 239000002131 composite material Substances 0.000 claims description 22
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 21
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 21
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 18
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 18
- 244000063299 Bacillus subtilis Species 0.000 claims description 16
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 16
- 229940088598 enzyme Drugs 0.000 claims description 16
- 108090000526 Papain Proteins 0.000 claims description 13
- 235000019834 papain Nutrition 0.000 claims description 13
- 229940055729 papain Drugs 0.000 claims description 13
- 108091005508 Acid proteases Proteins 0.000 claims description 12
- 235000019764 Soybean Meal Nutrition 0.000 claims description 10
- 238000009629 microbiological culture Methods 0.000 claims description 10
- 239000004455 soybean meal Substances 0.000 claims description 10
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 8
- 238000011160 research Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 241000186660 Lactobacillus Species 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 230000000813 microbial effect Effects 0.000 claims description 5
- 235000014655 lactic acid Nutrition 0.000 claims description 4
- 239000004310 lactic acid Substances 0.000 claims description 4
- 235000019750 Crude protein Nutrition 0.000 claims description 3
- 102000057297 Pepsin A Human genes 0.000 claims description 3
- 108090000284 Pepsin A Proteins 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 229940111202 pepsin Drugs 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 8
- 108090000623 proteins and genes Proteins 0.000 abstract description 8
- 238000000855 fermentation Methods 0.000 abstract description 6
- 238000012545 processing Methods 0.000 abstract description 6
- 241001465754 Metazoa Species 0.000 abstract description 5
- 244000144972 livestock Species 0.000 abstract description 5
- 244000144977 poultry Species 0.000 abstract description 5
- 108091005804 Peptidases Proteins 0.000 abstract description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 4
- 238000003860 storage Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 230000002496 gastric effect Effects 0.000 abstract description 2
- 235000013330 chicken meat Nutrition 0.000 description 46
- 238000004321 preservation Methods 0.000 description 9
- 235000018102 proteins Nutrition 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 235000013594 poultry meat Nutrition 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 241001494479 Pecora Species 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 235000019419 proteases Nutrition 0.000 description 3
- 210000004767 rumen Anatomy 0.000 description 3
- 239000004460 silage Substances 0.000 description 3
- 238000003307 slaughter Methods 0.000 description 3
- 229920002261 Corn starch Polymers 0.000 description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000008120 corn starch Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 1
- 241000193749 Bacillus coagulans Species 0.000 description 1
- 108010051479 Bombesin Proteins 0.000 description 1
- 102000013585 Bombesin Human genes 0.000 description 1
- 241000252983 Caecum Species 0.000 description 1
- 101800001982 Cholecystokinin Proteins 0.000 description 1
- 102100025841 Cholecystokinin Human genes 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 102000055135 Vasoactive Intestinal Peptide Human genes 0.000 description 1
- 108010003205 Vasoactive Intestinal Peptide Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000000184 acid digestion Methods 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229940054340 bacillus coagulans Drugs 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- DNDCVAGJPBKION-DOPDSADYSA-N bombesin Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(N)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CC=1NC2=CC=CC=C2C=1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1NC(=O)CC1)C(C)C)C1=CN=CN1 DNDCVAGJPBKION-DOPDSADYSA-N 0.000 description 1
- 150000005693 branched-chain amino acids Chemical class 0.000 description 1
- 210000004534 cecum Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229940107137 cholecystokinin Drugs 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
- VBUWHHLIZKOSMS-RIWXPGAOSA-N invicorp Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)C(C)C)[C@@H](C)O)[C@@H](C)O)C(C)C)C1=CC=C(O)C=C1 VBUWHHLIZKOSMS-RIWXPGAOSA-N 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 210000000713 mesentery Anatomy 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 229940081969 saccharomyces cerevisiae Drugs 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a chicken intestine fermented feed raw material and a preparation method thereof. The preparation method of the chicken intestine fermented feed raw material comprises the following steps: 1) pretreating raw materials, and mincing fresh chicken intestines to prepare mashed intestines; 2) compounding, namely compounding the intestinal mud obtained in the step 1) with wheat bran, bean pulp, compound enzyme and compound inoculation liquid, and uniformly mixing; 3) bagging and fermenting, namely filling the materials compounded in the step 2) into a plastic bag, exhausting air in the bag, sealing, and fermenting for 7-10 days at room temperature. The invention has the following advantages: 1. by adopting a method of bacteria enzyme cooperated with anaerobic fermentation, the product can be stored for a long time, and the storage and transportation problems of fresh chicken intestines are overcome; 2. the prepared fermented chicken gastrointestinal protease has high digestibility and is rich in small peptide components; 3. the production facility and the process are simple, and the local processing of the chicken sausage can be realized. The fermented chicken intestine can be directly fed to various livestock, poultry and aquatic animals, or partially replace protein feed raw materials or complete feed.
Description
Technical Field
The invention belongs to the technical field of animal feed, and relates to a protein feed raw material obtained by fermenting chicken intestines and a preparation method thereof.
Background
The chicken intestines account for 1.5-2% of the live weight of the chicken and are one of main leftovers of a broiler slaughter house. Researches show that the fresh chicken intestinal protein and essential amino acid are high in content, particularly contain lysine, arginine, leucine and other branched chain amino acids, and also contain trace P substances, bombesin, vasoactive intestinal peptide, cholecystokinin and other active substances, so that the chicken intestinal protein and leucine and other active substances have high nutritional values. At present, 100 hundred million broiler chickens are slaughtered in average year in China, the annual fresh chicken intestines can reach 400 million tons, and the broiler chickens are generally sold as slaughter wastes to freezing enterprises for temporary storage at low cost due to low edible value and then sold to farmers for directly feeding carnivorous fishes or fur-bearing animals. A small amount of chicken intestine powder is degreased or dried by a processing factory to be processed into chicken intestine powder, and the chicken intestine powder is sold as protein feed. However, the fresh chicken intestines are rich in protein, so that the fresh chicken intestines are extremely easy to decay in the turnover and transportation processes, the biological safety risk of fresh feeding is high, a refrigeration plant is not supervised by a feed production management department, and the potential safety hazard is high. On the other hand, the moisture content of the fresh chicken intestines reaches up to 70 percent, the drying processing energy consumption is high, the amino acid digestion rate is reduced, active substances are damaged, the nutritive value is reduced, and the industrial development of the chicken intestine powder is severely restricted.
Patent application CN 201910024038.5 discloses a fermented pig feed raw material for replacing soybean meal, which is prepared by fermenting chicken intestines, glutamic acid residues, bran, corn flour and a bacterial liquid, wherein the bacterial liquid consists of bacillus coagulans, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus, lactobacillus casei and lactobacillus plantarum. According to the results of comparing the nutrient components before and after fermentation, the protein degradation effect is not ideal.
In order to fully utilize the chicken intestine protein resources and improve the feeding value, the development of a chicken intestine processing technology which has low cost and mild conditions and is suitable for the local transformation in slaughterhouses is necessary, and the technical bottleneck of the storage and processing of the chicken intestines is overcome.
Disclosure of Invention
The invention aims to provide a small peptide-rich bacterial enzyme synergistic fermented chicken intestine feed raw material and a preparation method thereof.
The chicken intestine fermented feed raw material provided by the invention is prepared by the following steps:
1) pretreating raw materials, and mincing fresh chicken intestines to prepare mashed intestines;
2) compounding, namely compounding the intestinal mud obtained in the step 1) with wheat bran, bean pulp, compound enzyme and compound inoculation liquid, and uniformly mixing;
3) bagging and fermenting, namely filling the materials compounded in the step 2) into a plastic bag, exhausting air in the bag, sealing, and fermenting for 7-10 days at room temperature.
Further, the fresh chicken intestines in the step 1) are a mixture of chicken small intestines, rectum, caecum, glandular stomach and mesentery in any proportion.
Further, the mass part ratio of the intestinal mud, the wheat bran, the soybean meal, the complex enzyme and the composite inoculation liquid in the step 2) is 70-75: 5-10: 10-25: 0.01-0.03: 0.5-1.
Further, the complex enzyme in the step 2) comprises enzymes such as acid protease and papain.
Further, the content of the acidic protease is 5000U/kg to 30000U/kg.
Further, the acid protease may be specifically a high-temperature resistant acid protease.
Further, the high temperature resistant acid protease may be a high temperature resistant acid protease of Beijing-challenged biotechnology, Inc.
Furthermore, the content of the papain is 10000U/kg-50000U/kg.
Further, the papain can be solid food-grade papain.
Further, the papain can be solid food-grade papain FDG-2203 from Hiroshi Biotech development, Inc., of Beijing.
Further, the compound inoculation liquid in the step 2) comprises probiotics such as lactic acid bacteria, bacillus, saccharomyces cerevisiae and the like, brown sugar and water.
Further, the content of lactic acid bacteria in the composite inoculation liquid is 2 x 1010CFU/kg～2×1011CFU/kg。
Further, in the composite inoculation liquid, the lactobacillus can be specifically lactobacillus acidophilus LA (with the preservation registration number of CGMCC No. 14437).
The Lactobacillus acidophilus (Lactobacillus acidophilus) strain LA provided by the invention is separated from fermented corn silage. The strain is preserved in China general microbiological culture Collection center (CGMCC for short, with the address of No. 3 Xilu-Beijing university of Tokyo facing Yang district, No. 3 Microbiol research institute of China academy of sciences) at 17.07.7.7.7.8.37, and the preservation registration number is CGMCC No. 14437.
Lactobacillus acidophilus (Lactobacillus acidophilus) CGMCC No.14437, herein abbreviated as Lactobacillus acidophilus LA.
Lactobacillus acidophilus (Lactobacillus acidophilus) CGMCC No.14437 was earlier disclosed in patent application CN 201711400534.3.
Further, the content of bacillus in the composite inoculation liquid is 4 multiplied by 1010CFU/kg～4×1011CFU/kg。
Further, in the above composite inoculation liquid, the bacillus may be bacillus subtilis L6 (with the preservation registration number of CGMCC No. 18229).
The Bacillus subtilis strain L6 provided by the invention is separated from sheep rumen fluid. The strain is preserved in China general microbiological culture Collection center (CGMCC for short, with the address of No. 3 Xilu-Beijing university of Tokyo, Chaoyang district, China academy of sciences) within 15.07.15.2019, and the preservation registration number is CGMCC No. 18229.
Bacillus subtilis CGMCC No.18229, herein abbreviated as Bacillus subtilis L6.
Bacillus subtilis CGMCC No.18229 was earlier disclosed in patent application CN 202010663114. X.
Further, in the composite inoculation liquid, the content of the saccharomyces cerevisiae is 5 multiplied by 1010CFU/kg～5×1011CFU/kg。
Further, in the composite inoculation liquid, the saccharomyces cerevisiae can be a feeding high-activity dry yeast.
Further, in the composite inoculation liquid, the feeding high-activity dry yeast can be specifically fubang feeding high-activity dry yeast (special for livestock and poultry) of Angel Yeast GmbH.
Furthermore, the content of the brown sugar in the composite inoculation liquid is 30-60 g/kg.
The chicken intestine fermented feed prepared by the method is yellow brown, has acid and fragrant smell, and has the water content of not more than 55%, the crude protein content of not less than 25%, the small peptide content of not less than 20%, the pepsin digestion rate of not less than 93% and the total number of moulds of not more than 5000 CFU/g.
Specifically, the present invention is as follows.
1. A preparation method of chicken intestine fermented feed raw materials comprises the following steps:
1) pretreating raw materials, and mincing fresh chicken intestines to prepare mashed intestines;
2) compounding, namely compounding the intestinal mud obtained in the step 1) with wheat bran, bean pulp, compound enzyme and compound inoculation liquid, and uniformly mixing;
3) bagging and fermenting, namely filling the materials compounded in the step 2) into a plastic bag, exhausting air in the bag, sealing, and fermenting for 7-10 days at room temperature.
2. The method for preparing a chicken intestine fermented feed raw material according to the item 1, which is characterized by comprising the following steps: the mass part ratio of the intestinal mud, the wheat bran, the soybean meal, the complex enzyme and the composite inoculation liquid in the step 2) is 70-75: 5-10: 10-25: 0.01-0.03: 0.5-1 in sequence.
3. The method for preparing a fermented chicken intestine feed material according to item 1 or 2, wherein the fermented chicken intestine feed material comprises: the compound enzyme in the step 2) comprises acid protease and papain.
4. The method for preparing a chicken intestine fermented feed raw material according to the item 3, which is characterized by comprising the following steps: the content of the acid protease is 5000U/kg-30000U/kg, and the content of the papain is 10000U/kg-50000U/kg.
5. The method for preparing a chicken intestine fermented feed raw material according to any one of items 1 to 4, characterized by: the composite inoculation liquid in the step 2) comprises lactic acid bacteria, bacillus, saccharomyces cerevisiae, brown sugar and water.
6. The method for preparing a chicken intestine fermented feed raw material according to the item 5, which is characterized by comprising the following steps: the content of the lactobacillus in the composite inoculation liquid is 2 multiplied by 1010CFU/kg～2×1011CFU/kg。
7. The method for preparing a fermented chicken intestine feed material according to item 5 or 6, wherein the fermented chicken intestine feed material comprises: the lactobacillus is Lactobacillus acidophilus LA, the Lactobacillus acidophilus LA strain is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms (CGMCC) in 2017 at 17.07.7, and the address is as follows: the collection registration number of the microbial research institute of Chinese academy of sciences is CGMCC No.14437, No. 3 of Xilu No.1 of Beijing, Chaoyang, and Zhongyang.
8. The method for preparing a chicken intestine fermented feed raw material according to any one of claims 5 to 7, characterized by comprising: the content of the bacillus in the composite inoculation liquid is 4 multiplied by 1010CFU/kg～4×1011CFU/kg。
9. The method for preparing a chicken intestine fermented feed raw material according to any one of claims 5 to 8, characterized by comprising: the bacillus is bacillus subtilis L6, and the bacillus subtilis L6 strain is preserved in China general microbiological culture Collection center (CGMCC) in 2019, 07, 15 and addresses as follows: the collection registration number of the microbial research institute of Chinese academy of sciences is CGMCC No. 18229.
10. The method for preparing a chicken intestine fermented feed material according to any one of claims 5 to 9, characterized by comprising: the content of the saccharomyces cerevisiae in the composite inoculation liquid is 5 multiplied by 1010CFU/kg～5×1011CFU/kg。
11. The feed material obtained by the method for preparing a chicken intestine-fermented feed material according to any one of items 1 to 10, wherein the feed material has a crude protein content of not less than 25%, a small peptide content of not less than 20%, and a pepsin digestibility of not less than 93%.
The fermented chicken intestine feed raw material can be directly fed to various livestock, poultry and aquatic animals, or can be used as a feed raw material to prepare a complete feed to feed various livestock, poultry and aquatic animals, and the complete feed can partially replace a protein feed raw material or a complete feed.
The invention has the following advantages: 1. by adopting the microbial anaerobic fermentation method, the product can be stored for a long time, and the storage and transportation problems of the fresh chicken intestines are overcome. 2. The prepared fermented chicken gastrointestinal protease has high digestibility and is rich in small peptide components. 3. The production facility and the process are simple, the local processing of the chicken intestines can be realized, and the slaughtering waste is fully utilized as a feed resource.
Biological material preservation information
The Lactobacillus acidophilus (Lactobacillus acidophilus) strain LA provided by the invention is separated from fermented corn silage. The strain is preserved in China general microbiological culture Collection center (CGMCC for short, with the address of No. 3 Xilu-Beijing university of Tokyo facing Yang district, No. 3 Microbiol research institute of China academy of sciences) at 17.07.7.7.7.8.37, and the preservation registration number is CGMCC No. 14437.
The Bacillus subtilis strain L6 provided by the invention is separated from sheep rumen fluid. The strain is preserved in China general microbiological culture Collection center (CGMCC for short, with the address of No. 3 Xilu-Beijing university of Tokyo, Chaoyang district, China academy of sciences) within 15.07.15.2019, and the preservation registration number is CGMCC No. 18229.
Detailed Description
For a better understanding of the present invention, the following examples are given. It should be understood that the embodiments of the present invention are provided by way of illustration and not limitation, and the scope of the invention is limited only by the claims of the present invention. The embodiments of the present invention are merely some preferred embodiments, and those skilled in the art can make modifications and substitutions to form different embodiments according to the contents of the present invention. However, any changes and modifications, and any equivalents to the method of the present invention, which do not depart from the spirit of the present invention, are intended to be included within the scope of the present invention.
The present invention will be described below with reference to specific examples, but the present invention is not limited thereto.
The experimental methods used in the following examples are all conventional methods unless otherwise specified; the reagents and materials, unless otherwise specified, are available from commercial sources or the general microbiological center of the national Committee for culture Collection of microorganisms.
The acidic protease used in the following examples was purchased from Beijing Biotechnology GmbH and was available in the following product types: temperature-resistant acid protease (50000U/g).
Papain, available from Hirshi Biotech development Inc. (Beijing), was used in the following examples in the form of: FDG-2203 (100000U/g).
Lactobacillus acidophilus (Lactobacillus acidophilus) strain LA, used in the examples below, was isolated from fermented corn silage. The strain is preserved in China general microbiological culture Collection center (CGMCC for short, with the address of No. 3 Xilu-Beijing university of Tokyo facing Yang district, No. 3 Microbiol research institute of China academy of sciences) at 17.07.7.7.7.8.37, and the preservation registration number is CGMCC No. 14437.
The Bacillus subtilis strain L6 used in the examples described below was isolated from sheep rumen fluid. The strain is preserved in China general microbiological culture Collection center (CGMCC for short, with the address of No. 3 Xilu-Beijing university of Tokyo, Chaoyang district, China academy of sciences) within 15.07.15.2019, and the preservation registration number is CGMCC No. 18229.
The Saccharomyces cerevisiae used in the following examples was purchased from Angel Yeast, Inc. under the product types: the Fubang feed uses high-activity dry yeast (special for livestock and poultry).
Example 1
1. Pretreatment of raw materials
40 kg of fresh chicken intestines are minced into mashed intestine by a meat grinder; crushing the soybean meal, and sieving the crushed soybean meal with a 20-mesh sieve.
2. Compounding
Preparing a composite inoculation liquid: adding 100 ml distilled water into 25 ml bacterial suspension of Lactobacillus acidophilus LA (wherein the content of Lactobacillus acidophilus is 8 × 10)8CFU/ml), 10 ml of a bacterial suspension of Bacillus subtilis L6 (wherein the content of Bacillus subtilis is 4X 10)9CFU/ml), 2.5 g of feeding high-activity dry yeast (wherein, the content of yeast is 2 multiplied by 10)10CFU/g) and 20 g of brown sugar, mixing uniformly, adding distilled water continuously to dilute to 500 g, and stirring uniformly to obtain the composite inoculation liquid.
Weighing 35 kg of the obtained intestinal mud, 2.5 kg of wheat bran, 12 kg of soybean meal, 10 g of complex enzyme and 0.5 kg of complex inoculation liquid (the relative mass ratio is 70:5:24:0.02:1), and mixing in a paddle or ribbon mixer for 10 minutes to obtain a mixed material. The formula of the complex enzyme comprises 40% of acid protease, 50% of papain and 10% of corn starch.
3. Bagging fermentation
The mixed materials are put into plastic bags according to 10 kg per bag, compacted, sealed after air is extruded out, piled and fermented for 7 days at room temperature (15-22 ℃), the bags are opened, and chemical components are sampled and measured, and the results are shown in table 1.
TABLE 1 chemical analysis results of fermented chicken intestine feeds
Example 2
1. Pretreatment of raw materials
100 kg of fresh chicken intestines are minced into mashed intestine by a meat grinder; crushing the soybean meal, and sieving the crushed soybean meal with a 20-mesh sieve.
2. Compounding
Preparing a composite inoculation liquid: adding 500 ml distilled water into 100 ml lactobacillus acidophilus LA suspension (wherein, the content of lactobacillus acidophilus is 8 × 10)8CFU/ml), 30 ml of a bacterial suspension of Bacillus subtilis L6 (wherein the content of Bacillus subtilis is 4X 10)9CFU/ml), feed heightActive dry yeast 10 g (wherein, the content of yeast is 2 x 10)10CFU/g) and 50 g of brown sugar, mixing uniformly, adding distilled water continuously to dilute to 1kg, and stirring uniformly to obtain the composite inoculation liquid.
Weighing 75 kg of the obtained intestinal mud, 10 kg of wheat bran, 12 kg of soybean meal, 30g of complex enzyme and 1kg of complex inoculation liquid (the relative mass ratio is 75:10:12:0.03:1), and mixing in a paddle or ribbon mixer for 10 minutes to obtain a mixed material. The formula of the complex enzyme comprises 40% of acid protease, 50% of papain and 10% of corn starch.
3. Bagging fermentation
And (3) putting the mixed materials into plastic bags according to 20 kg per bag, compacting, extruding air, sealing, stacking at room temperature (15-22 ℃) for fermentation for 7 days, opening the bags, sampling and measuring chemical components, wherein the results are shown in table 2.
TABLE 2 chemical analysis results of fermented chicken intestine feeds
Claims (10)
1. A preparation method of chicken intestine fermented feed raw materials comprises the following steps:
1) pretreating raw materials, and mincing fresh chicken intestines to prepare mashed intestines;
2) compounding, namely compounding the intestinal mud obtained in the step 1) with wheat bran, bean pulp, compound enzyme and compound inoculation liquid, and uniformly mixing;
3) bagging and fermenting, namely filling the materials compounded in the step 2) into a plastic bag, exhausting air in the bag, sealing, and fermenting for 7-10 days at room temperature.
2. The method for preparing a chicken intestine fermented feed raw material according to claim 1, wherein the method comprises the following steps: the mass part ratio of the intestinal mud, the wheat bran, the soybean meal, the complex enzyme and the composite inoculation liquid in the step 2) is 70-75: 5-10: 10-25: 0.01-0.03: 0.5-1 in sequence.
3. The method for preparing a chicken intestine fermented feed material according to claim 1 or 2, wherein: the compound enzyme in the step 2) comprises acid protease and papain.
4. The method for preparing a chicken intestine fermented feed raw material according to claim 3, wherein the method comprises the following steps: the content of the acid protease is 5000U/kg-30000U/kg, and the content of the papain is 10000U/kg-50000U/kg.
5. The method for preparing a chicken intestine fermented feed material according to any one of claims 1 to 4, wherein: the composite inoculation liquid in the step 2) comprises lactic acid bacteria, bacillus, saccharomyces cerevisiae, brown sugar and water.
6. The method for preparing a chicken intestine fermented feed raw material according to claim 5, wherein the method comprises the following steps: the content of the lactobacillus in the composite inoculation liquid is 2 multiplied by 1010CFU/kg～2×1011CFU/kg。
7. The method for preparing a chicken intestine fermented feed material according to claim 5 or 6, wherein the method comprises the following steps: the lactobacillus is Lactobacillus acidophilus LA, the Lactobacillus acidophilus LA strain is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms (CGMCC) in 2017 at 17.07.7, and the address is as follows: the collection registration number of the microbial research institute of Chinese academy of sciences is CGMCC No.14437, No. 3 of Xilu No.1 of Beijing, Chaoyang, and Zhongyang.
8. The method for preparing a chicken intestine fermented feed material according to any one of claims 5 to 7, wherein: the content of the bacillus in the composite inoculation liquid is 4 multiplied by 1010CFU/kg～4×1011CFU/kg。
9. The method for preparing a chicken intestine fermented feed material according to any one of claims 5 to 8, wherein: the bacillus is bacillus subtilis L6, and the bacillus subtilis L6 strain is preserved in China general microbiological culture Collection center (CGMCC) in 2019, 07, 15 and addresses as follows: the collection registration number of the microbial research institute of Chinese academy of sciences is CGMCC No. 18229.
10. The raw feed material obtained by the method for preparing a fermented chicken intestine feed material according to any one of claims 1 to 9, which has a crude protein content of not less than 25%, a small peptide content of not less than 20%, and a pepsin digestibility of not less than 93%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110488610.0A CN113080312A (en) | 2021-04-26 | 2021-04-26 | Small peptide-rich bacterium-enzyme synergistic fermented chicken sausage and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110488610.0A CN113080312A (en) | 2021-04-26 | 2021-04-26 | Small peptide-rich bacterium-enzyme synergistic fermented chicken sausage and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113080312A true CN113080312A (en) | 2021-07-09 |
Family
ID=76681685
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110488610.0A Pending CN113080312A (en) | 2021-04-26 | 2021-04-26 | Small peptide-rich bacterium-enzyme synergistic fermented chicken sausage and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113080312A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931887A (en) * | 2014-04-14 | 2014-07-23 | 中国农业科学院饲料研究所 | Broccoli stem and leaf fermented feed and preparation method thereof |
| CN105211827A (en) * | 2015-11-02 | 2016-01-06 | 湖南省蜀中情食品有限公司 | A kind of method utilizing chicken intestines to prepare flavouring |
| CN105815551A (en) * | 2016-03-25 | 2016-08-03 | 武汉力田生物科技有限公司 | Method for preparing nonreactive environment-friendly feed through synergistic fermentation of bacterium enzymes |
| CN107974421A (en) * | 2017-12-22 | 2018-05-01 | 中国农业科学院饲料研究所 | A kind of lactobacillus acidophilus and its screening technique and application, a kind of microbial inoculum |
| CN109845880A (en) * | 2019-01-10 | 2019-06-07 | 河南爱猪人生物科技股份有限公司 | A kind of fermentation pannage of Substitution for Soybean Meal |
| CN111436526A (en) * | 2020-04-01 | 2020-07-24 | 浙江大学 | Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs |
| CN111690568A (en) * | 2020-07-10 | 2020-09-22 | 中国农业科学院饲料研究所 | Bacillus subtilis and application thereof in fermentation treatment of detoxication of flaxseed cake |
| CN111838408A (en) * | 2020-07-27 | 2020-10-30 | 山东百德生物科技有限公司 | Functional amino acid enzyme and preparation method thereof |
-
2021
- 2021-04-26 CN CN202110488610.0A patent/CN113080312A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931887A (en) * | 2014-04-14 | 2014-07-23 | 中国农业科学院饲料研究所 | Broccoli stem and leaf fermented feed and preparation method thereof |
| CN105211827A (en) * | 2015-11-02 | 2016-01-06 | 湖南省蜀中情食品有限公司 | A kind of method utilizing chicken intestines to prepare flavouring |
| CN105815551A (en) * | 2016-03-25 | 2016-08-03 | 武汉力田生物科技有限公司 | Method for preparing nonreactive environment-friendly feed through synergistic fermentation of bacterium enzymes |
| CN107974421A (en) * | 2017-12-22 | 2018-05-01 | 中国农业科学院饲料研究所 | A kind of lactobacillus acidophilus and its screening technique and application, a kind of microbial inoculum |
| CN109845880A (en) * | 2019-01-10 | 2019-06-07 | 河南爱猪人生物科技股份有限公司 | A kind of fermentation pannage of Substitution for Soybean Meal |
| CN111436526A (en) * | 2020-04-01 | 2020-07-24 | 浙江大学 | Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs |
| CN111690568A (en) * | 2020-07-10 | 2020-09-22 | 中国农业科学院饲料研究所 | Bacillus subtilis and application thereof in fermentation treatment of detoxication of flaxseed cake |
| CN111838408A (en) * | 2020-07-27 | 2020-10-30 | 山东百德生物科技有限公司 | Functional amino acid enzyme and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王世宽 等: "鸡肠蛋白酶解多肽功能特性研究", 食品科技, vol. 43, no. 8, pages 239 - 244 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102524535B (en) | Oligopeptide additive for feed and preparation method thereof | |
| CN105614023B (en) | Fermentation enzymolysis agent for soybean meal fermentation and application thereof | |
| CN103907747B (en) | A kind of high-protein feeding preparation method for material | |
| CN102232466A (en) | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof | |
| CN101999527B (en) | Immune feed additive and preparation method thereof | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| CN111838408A (en) | Functional amino acid enzyme and preparation method thereof | |
| CN107801837B (en) | Biological fermentation process for forage-based utilization of chicken manure | |
| CN105613944A (en) | Fermented soybean meal, production method thereof, and application of fermented soybean meal in preparation of compound feed for pigs | |
| CN105707434A (en) | Method and application of fermented biological feed prepared by multi-strain mixed fermentation | |
| CN105876082A (en) | Peptide protein feed and preparation method thereof | |
| CN105851479A (en) | Earthworm fermentation liquid, and preparation method and application method thereof | |
| CN107279464B (en) | Process for engineering fermentation of biomass feed | |
| CN109123221B (en) | Total nutrient aquaculture feed prepared from aquatic product processing leftovers | |
| CN102084929B (en) | Biological feed protein and production system and application thereof | |
| CN101449741B (en) | Multifunctional biology corn feedstuff | |
| CN110301526A (en) | Complex micro organism fungicide and its method for preparing bioactive feed | |
| CN113414226A (en) | Kitchen waste leavening agent and preparation method and application thereof | |
| CN102318736B (en) | Fermented feed protein and processing method thereof | |
| CN102813055A (en) | EM (effective microorganisms) fermented straw high-protein mixed feed and production method thereof | |
| CN113080312A (en) | Small peptide-rich bacterium-enzyme synergistic fermented chicken sausage and preparation method thereof | |
| CN108094733A (en) | It is a kind of to prepare the method for livestock feed additive and livestock feed additive by raw material of mushroom bran | |
| CN114886008A (en) | Biological fermentation selenium-rich feed and preparation method thereof | |
| CN112586615A (en) | Preparation method of beef cattle feed | |
| CN110419622B (en) | Composite probiotic oligosaccharide fruit feed additive and preparation and use methods thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |