CN113080307A

CN113080307A - Method for producing feed by using amino acid fermentation mother liquor and corn sugar residues

Info

Publication number: CN113080307A
Application number: CN202110401995.2A
Authority: CN
Inventors: 孙开济; 胡炎华; 李岩
Original assignee: Langfang Meihua Bio Technology Development Co Ltd
Current assignee: Langfang Meihua Bio Technology Development Co Ltd
Priority date: 2021-04-14
Filing date: 2021-04-14
Publication date: 2021-07-09
Anticipated expiration: 2041-04-14
Also published as: CN113080307B

Abstract

The invention relates to the field of feeds, in particular to a method for producing a feed by using amino acid fermentation mother liquor and corn sugar residues. The method comprises the following steps: mixing the amino acid fermentation mother liquor with the corn sugar residues, and then drying and granulating; wherein the dry matter content of the amino acid mother liquor is 40-55 wt%; the corn sugar residue is wet sugar residue or dry sugar residue, and the dry matter content of the wet sugar residue is 35-50 wt%; the water content of the dry sugar residue is 4-15 wt%; the mixing ratio of the amino acid fermentation mother liquor to the wet sugar residue and the dry sugar residue is respectively 0.8-1.2: 1 and 1.5-2.5: 1. the method of the invention not only can provide cheap high-energy high-protein feed for feed enterprises and breeding enterprises, but also can bring considerable economic benefit for amino acid fermentation enterprises, and simultaneously can reduce incineration pollution and reduce the risks of oxidative deterioration, even burning fire and the like in the drying and storage of the corn sugar residues to a certain extent.

Description

Method for producing feed by using amino acid fermentation mother liquor and corn sugar residues

Technical Field

The invention relates to the field of feeds, in particular to a method for producing a feed by using amino acid fermentation mother liquor and corn sugar residues.

Background

With the development of genetic engineering technology, most amino acid production technologies have been shifted from traditional methods such as chemical synthesis, protein hydrolysis, etc. to microbial fermentation. The production cost of the amino acid is gradually reduced along with the optimization of the strain improvement and the fermentation process, so that the supplement of the crystal amino to replace part of protein feed in the livestock breeding can bring more profits for breeding enterprises. The fermented amino acid is commonly used in the feed applying the low-protein daily ration technology, so that the feed cost is reduced, the diarrhea rate can be reduced on the premise of not influencing the growth performance of livestock and poultry, and the proportion of nitrogen elements in excrement is reduced, so that the environmental protection pressure of breeding enterprises is relieved to a certain extent.

The production of the fermented amino acid mainly takes glucose hydrolyzed by corn starch as a carbon source and ammonia as a nitrogen source, utilizes corynebacterium glutamicum or escherichia coli to ferment, and separates amino acid crystals after processes such as filtering and concentrating fermentation liquor. Although the biological fermentation method is the most green and environment-friendly mode for producing amino acid at present, the high-concentration fermentation sewage, called amino acid fermentation mother liquor, is still inevitably generated. The amino acid fermentation mother liquor is different from common industrial wastewater, and feed-grade or food-grade raw materials are used in the whole fermentation process, so that the amino acid fermentation mother liquor still has higher feeding value. In addition, the amino acid fermentation mother liquor contains abundant amino acid, soluble protein and carbohydrate which is not utilized by microorganisms, and has higher feeding value. Although the amino acid fermentation mother liquor has high feeding value, the amino acid fermentation mother liquor cannot be effectively dried due to the characteristics of high viscosity, strong hygroscopicity and the like, so that the amino acid fermentation mother liquor cannot be widely used in the transportation and feed processing processes. At present, the main treatment method of amino acid fermentation mother liquor is incineration, which not only increases the cost for enterprises, but also causes pollution to a certain extent, so that the problem of drying the amino acid mother liquor is necessary.

The carbon source for amino acid fermentation mainly comes from glucose hydrolyzed from corn starch, and a residue-like by-product containing protein and fat as main components is inevitably generated in the process of starch saccharification, and is called corn sugar residue. The corn bagasse is generated because corn protein and germ fragments, which are not completely separated during the corn starch extraction process, enter the saccharification process and are finally removed by filtration in the form of insoluble materials. Due to the fact that the sugar residues contain fat with a high proportion, the dried products are easy to oxidize and rancid in the storage and transportation processes, and spontaneous combustion occurs under the condition of stacking or poor heat dissipation. Therefore, currently, corn deep processing enterprises generally sell the filtered sugar residues directly without drying, which results in a great increase of transportation cost.

For the amino acid fermentation mother liquor, it is mentioned in CN111758840A of the applicant's previous application that the threonine fermentation mother liquor can be viscosity-reduced by adding corncob powder, silicon dioxide, calcium carbonate, etc., and the treated concentrated mother liquor can be dried by a spray dryer or a fluidized bed, and a powdered or granular feed product is produced, but the strong moisture absorption of the threonine mother liquor causes the process to have relatively high production requirements, requires continuous operation of equipment, has high requirements on packaging, and requires sealed packaging, which results in relatively high production cost. Other patents (such as CN109694828A) mainly produce mycoprotein and fertilizer by adding strains such as candida utilis, lactobacillus casei, houttuynia cordata and the like and fermenting for 10-14 days, although the method can exert the feeding value of amino acid mother liquor to a certain extent, the technical operation is complex, multi-step operation and cultivation and domestication of a plurality of strains are required, meanwhile, the technical fermentation treatment time is long, the factory space is occupied for a long time, higher labor cost and equipment depreciation cost are consumed, and the product has the characteristic of lower profit compared with the production cost, so the method is not widely used.

Although some patents (CN1879496A) show that corn pomace can be dried by air flow and sold as pellet feed, the current way of handling corn pomace is mainly to sell wet undried pomace directly because the high fat content in the pomace causes a relatively high risk of oxidative deterioration in the storage stage after drying. The wet sugar residues contain about 60 percent of water, so the storage time and the transportation distance are limited, and due to the limitation of corn supply, the corn deep processing enterprises are usually provided with few large-scale livestock and poultry farms, so the corn sugar residues can be used as organic fertilizers (CN103265352A) or feed in a small amount in a low-value mode.

In addition, CN 109793097a mentions a fermentation method for producing biological feed by using sugar-making tailings and tryptophan mother liquor, wherein the tailings produced in the sugar-making process, corn steep liquor, corn bran, sugar residues and concentrated mother liquor produced in the tryptophan production process are subjected to facultative anaerobic fermentation and anaerobic fermentation by different strains to prepare the feed. Although the method solves the problem of the downstream processing difficulty of the sugar-making tailings and the tryptophan mother liquor in the tryptophan production process, the corn bran used in the method belongs to relatively high-value feed raw materials, and the overall value of the product can be reduced after the corn bran is mixed with relatively low-value raw materials such as corn steep liquor, tryptophan mother liquor and the like. In addition, the method needs various raw materials and fermentation steps, so that the problems of long production process, high production cost and the like are caused, and the method is not suitable for actual industrial production.

CN 102578385A proposes a method for preparing low-content L-threonine feed, which uses trapped fluid and mother liquor generated in the preparation process of high-content L-threonine feed as raw materials and uses three feed additives of corn fiber, corn protein and light calcium as auxiliary materials to prepare high-quality low-content L-threonine feed, thereby solving the problem that the trapped fluid and the mother liquor pollute the environment.

Disclosure of Invention

The invention discovers that after the fermentation mother liquor of lysine, threonine and the like which takes escherichia coli or corynebacterium glutamicum as engineering bacteria is concentrated, sugar liquor which is not completely utilized in the fermentation mother liquor can be synchronously concentrated, and in addition, various water-retaining substances resisting the hypertonic environment in the fermentation culture medium and the thallus are also concentrated, so that the amino acid mother liquor is high in viscosity in the drying process and high in hygroscopicity of the dried product. However, the main components of alcohol-soluble protein, starch and fat of the amino acid fermentation enterprises are hydrophobic through analysis of the components of the corn deep processing production line, and the sugar residues produced in the starch saccharification working section of the corn deep processing production line have higher oxidative deterioration and even heating spontaneous combustion risks in the drying, storage, transportation and use processes due to high fat content, so that the value of the corn deep processing production line cannot be fully exerted. Therefore, the invention not only can solve the wall sticking phenomenon and the moisture absorption phenomenon of the amino acid mother liquor in the drying process, but also can simultaneously solve the characteristics of easy oxidation rancidity and spontaneous combustion caused by high fat content of the corn pomace by controlling the dry matters (or water contents) of the two byproducts and mixing the dry matters in a specific ratio and drying.

Based on the above findings, the present invention provides, first of all, a method for producing a feed, comprising:

mixing the amino acid fermentation mother liquor with the corn sugar residues, and then drying and granulating;

wherein the dry matter content of the amino acid mother liquor is 40-55 wt%;

the corn sugar residue is wet sugar residue or dry sugar residue, and the dry matter content of the wet sugar residue is 35-50 wt%; the water content of the dry sugar residues is 4-15 wt%;

when the mixing is carried out, the mass ratio of the amino acid fermentation mother liquor to the wet sugar residues is 0.8-1.2: 1, the mass ratio of the amino acid fermentation mother liquor to the dry sugar residues is 1.5-2.5: 1.

preferably, the amino acid fermentation mother liquor is obtained by fermenting escherichia coli serving as an engineering bacterium or corynebacterium glutamicum, and is preferably lysine fermentation mother liquor or threonine fermentation mother liquor.

Preferably, when the dry matter content of the amino acid fermentation mother liquor is lower than 40 wt%, a triple-effect evaporator is used for evaporating and concentrating the amino acid fermentation mother liquor, and the setting conditions of the triple-effect evaporator are that the first-effect evaporation temperature is 85-90 ℃, the second-effect evaporation temperature is 65-70 ℃, and the triple-effect evaporation temperature is 55-60 ℃.

More preferably, the first-effect evaporation temperature is 86-88 ℃, the second-effect evaporation temperature is 68-70 ℃ and the third-effect evaporation temperature is 56-59 ℃.

After the concentration is carried out by the method, the subsequent drying cost is more favorably reduced.

In some embodiments, the amino acid fermentation liquor is subjected to evaporation concentration by using a multi-effect evaporator after bacteria are filtered by a ceramic membrane or a microfiltration and nanofiltration device. And cooling and crystallizing the concentrated solution, and separating by a separator to obtain the amino acid fermentation mother liquor.

Preferably, the method for obtaining the dry sugar residues comprises the following steps:

the wet sugar residues are crushed to the particle size of less than 1mm and then subjected to air flow drying at the temperature of 150-170 ℃.

Preferably, in the dry sugar residues, 30-40 wt% of crude protein, 20-30 wt% of crude fat and 0.5-2 wt% of ash can be totally 24.2-28.5 MJ/kg.

Preferably, the method comprises:

mixing the amino acid fermentation mother liquor, the wet sugar residues and at least one of strong base and emulsifier, and then drying and granulating;

the alkali is one or more of potassium hydroxide, sodium hydroxide and quicklime, and the emulsifier is one or more of lecithin, monoglyceride and sucrose ester;

based on the total weight of the mixture, the adding proportion of the strong base is 1.5-2 wt%, and the adding proportion of the emulsifier is 0.2-1.3 wt%.

The invention further discovers that the emulsifying property of fat in the sugar residue can be further increased by adding the strong base and the emulsifier, so that the fat component can be better mixed with the amino acid mother liquor, and the moisture absorption performance of a dry product after mixing can be reduced to a greater extent.

Wherein, one of strong base and emulsifier can be added to meet the process requirement, and the effect is better when the two are added together.

Preferably, the mixing is carried out at 55-65 ℃ and a pH of 5.0-8.0.

More preferably, when the strong base is not contained in the raw materials, the mixing is performed at a pH of 5.5 to 6.5; when the raw materials contain a strong base, the mixing is carried out at a pH of 6.5 to 8.0.

As a preferred embodiment, when the raw materials contain wet sugar residues, the stirring time is 4-6 h; when the raw materials contain dry sugar residues, the stirring time is 1-2 h.

Preferably, the mixed feed liquid is dried after removing particles of 1mm or more (which can be removed by filtration).

As a preferred aspect of the present invention, the method comprises the steps of:

mixing the amino acid fermentation mother liquor and the wet sugar residues, and then carrying out spray drying on the feed liquid, wherein the air flow pressure of a nozzle is set to be 0.3-0.5Mpa, the air inlet temperature is 110-130 ℃, the air outlet temperature is 65-75 ℃, and the water content of the product is controlled to be 4-6 wt%.

In actual operation, the person in the art can control the moisture of the product by adjusting factors such as the hot air inlet temperature, the air outlet temperature, the air quantity, the feed liquid flow and the like.

mixing the amino acid fermentation mother liquor and the dry sugar residues, drying the feed liquid by a fluidized bed drying technology, controlling the grain diameter of a bottom material to be 0.2-0.3mm, controlling the airflow pressure of a nozzle to be 0.5-0.6MPa, controlling the air inlet temperature to be 90-110 ℃, controlling the air outlet temperature to be 60-70 ℃, discharging after the grain diameter is increased to 2-3mm, and controlling the water content of a product to be 3-5 wt%.

mixing amino acid fermentation mother liquor, wet sugar residues and at least one of strong base and emulsifier, drying the feed liquid by a drum drying technology, controlling the surface temperature of a drum to be 120-130 ℃, the thickness of pulp coating to be 1.5-2mm, the rotating speed of the drum to be 0.5-1r/min, controlling the water content of a product to be 5-7 wt%, and crushing the product into particles with the particle size of 1.0-2.0 mm.

In practice, one skilled in the art can control the moisture content of the product by adjusting the surface temperature of the hot drum, the coating thickness, the rotation speed of the drum, and other factors.

The first method is the simplest and most feasible method, and is suitable for amino acid mother liquor with relatively low viscosity such as lysine, tryptophan and the like, the second method is different from the first method in equipment investment cost and production convenience because sugar residues need to be dried firstly, but the dry sugar residues have the tendency of absorbing water after being mixed with amino acid fermentation concentrated solution, so that the amino acid mother liquor is more beneficial to entering the interior of the sugar residues, and the amino acid mother liquor with high viscosity (particularly threonine mother liquor) is physically separated to a certain extent, so that the drying speed is higher in the drying process, and the moisture absorption rate of the dried product is lower. The third method is between the first two methods, the production process is relatively simple, and the drying speed of the feed liquid is obviously higher than that of the first method, but the hygroscopicity of the product is similar to that of the first method. Therefore, the three methods have advantages and disadvantages, and corresponding production modes can be selected as appropriate when different types of amino acid mother liquor are processed so as to achieve the highest production efficiency and the stability of products.

The above-described preferred embodiments may be combined by one skilled in the art to provide preferred embodiments of the present invention.

The invention also provides a feed prepared by the method.

Preferably, the feed comprises 45-60 wt% crude protein, 10-20 wt% crude fat, 3-6 wt% crude ash and 3-10 wt% water.

The product of the invention is energy protein feed which takes protein and fat as main components, has low hygroscopicity and has wide animal production application value.

The invention also provides the application of the method in any one of the following aspects:

(1) the application in the treatment or reuse of amino acid fermentation mother liquor;

(2) the application in the treatment or the reutilization of the corn sugar residue.

Based on the technical scheme, the invention at least has the following beneficial effects:

the method of the invention not only can provide cheap high-energy high-protein feed for feed enterprises and breeding enterprises, but also can bring considerable economic benefit for amino acid fermentation enterprises, and simultaneously can reduce incineration pollution and reduce the risks of oxidative deterioration, even burning fire and the like in the drying and storage of the corn sugar residues to a certain extent. In addition, the method is simple and easy to implement, and the prepared finished product has low hygroscopicity, is convenient to store and transport, and is more favorable for popularization and application in actual production.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or instruments used are conventional products available from regular distributors, not indicated by the manufacturer.

All the "%" mentioned in the following examples are "wt%".

Example 1

The embodiment provides a method for producing low-hygroscopicity granular energy and protein feed by using lysine mother liquor and wet sugar residues, which comprises the following steps:

(1) evaporating and concentrating the lysine mother liquor by a triple-effect evaporator, wherein the specific parameters comprise that the first-effect evaporation temperature is controlled at 88 ℃, the second-effect evaporation temperature is controlled at 69 ℃, and the triple-effect evaporation temperature is controlled at 58 ℃ until the dry matter content of the lysine mother liquor is 42.5%, and removing crystals and residues precipitated after concentration;

(2) and (3) mixing the concentrated lysine mother liquor with the wet sugar residues with the dry matter of 39.7% in a mass ratio of 1.1: 1 in a size mixing tank, stirring for 5 hours at 60 ℃, and controlling the pH value to be between 5.5 and 6.0;

(3) feeding the prepared feed liquid to the nozzle at a speed of 2.5L/h by screw pump, and maintaining the pressure of the air flow at 0.4MPa and 60m³The flow rate of inlet air is 125 ℃, the temperature of inlet air is 77 ℃, and the dried powder is recycled by cyclone. In the spray drying process of this example, no dry powder clogging occurredThe problems of spray head, equipment inner wall adhesion and the like occur. The main parameters of the spray-dried product are shown in table 1 below.

TABLE 1 analysis of nutrient composition of the product obtained in example 1

Index (I)	Content (wt.)
		Moisture content	4.7％
Crude protein	51.3％
		Crude fat	14.9％
Coarse ash content	4.9％

Example 2

The embodiment provides a method for producing low-hygroscopicity granular energy and protein feed by using threonine mother liquor and dry sugar residues, which comprises the following steps:

(1) evaporating and concentrating the threonine mother liquor by a triple-effect evaporator, wherein the specific parameters are that the first-effect evaporation temperature is controlled at 88 ℃, the second-effect evaporation temperature is controlled at 70 ℃, the triple-effect evaporation temperature is controlled at 60 ℃, the content of concentrated dry matters is 51.5%, and amino acid crystals and other impurities are filtered and removed;

(2) drying the wet sugar residues with the dry matter content of 45.4% by using an airflow dryer at 165 ℃ to obtain a finished product with the water content of 8.4%;

(3) and (3) mixing the concentrated threonine mother liquor with the dried sugar residues according to the mass ratio of 2: 1 in a size mixing tank, stirring for 1h at 60 ℃, and controlling the pH value to be between 5.3 and 5.8;

(4) adding base material with particle diameter of 0.2mm into fluidized bed, feeding the prepared material liquid to fluidized bed nozzle via screw pump at 4L/h rate, and maintaining nozzle airflow pressure at 0.6MPa and 80m³The flow rate of air inlet per hour, the air inlet temperature of 90 ℃ and the air outlet temperature of 60 ℃, tail gas is recycled by cyclone, and recycled powder is reversely put into the fluidized bed. Discharging when the particles grow to about 2mm, cooling and packaging. In the fluidization granulation process of the embodiment, a series of problems caused by excessive viscosity of the feed liquid do not occur. The main parameters of the fluidized granulation product are given in table 2 below.

Table 2 analysis table of nutrient composition of product obtained in example 2

Index (I)	Content (%)
		Moisture content	3.2％
Crude protein	55.7％
		Crude fat	13.1％
Coarse ash content	4.3％

Example 3

The embodiment provides a method for producing low-hygroscopicity granular energy protein feed by using threonine mother liquor and wet sugar residues under the condition of adding an auxiliary agent, which comprises the following steps:

(1) evaporating and concentrating the threonine mother liquor by a triple-effect evaporator, wherein the specific parameters comprise that the single-effect evaporation temperature is controlled to be 85 ℃, the double-effect evaporation temperature is controlled to be 63 ℃, the triple-effect evaporation temperature is controlled to be 50 ℃, the content of concentrated dry matters is 47.6%, and filtering is carried out to remove precipitated amino acid crystals and other impurities;

(2) mixing the concentrated threonine mother liquor with wet sugar residues with dry matters of 36.9% in a mass ratio of 1: 1, mixing in a size mixing tank, adding 1 percent of quicklime and 0.5 percent of sucrose ester, stirring for 6 hours at 60 ℃, and controlling the pH to be between 6.5 and 7.3;

(3) using a drum dryer, adjusting the surface temperature of a drum to 125 ℃, distributing the material with the thickness of 1.5mm at the rotating speed of 1r/min, sending the prepared material liquid to a distributing plate at the speed of 50L/h by a screw pump, hanging the dried material by a scraper, crushing the dried material into particles with the particle size of about 1mm, cooling and packaging. A series of problems that the feed liquid is stuck on the drum wall, a scraper is stuck, and the grinding is not easy to happen in the production process do not occur. The main parameters of the drum dried product are shown in table 3 below.

Table 3 analysis table of nutrient composition of product obtained in example 3

Comparative example 1

The comparative example is different from example 1 only in that the wet sugar residue in step (2) is replaced by equal weight of corn husk powder which is dried and then crushed to below 40 meshes, and the rest production steps are the same. The main parameters of the spray-dried product are shown in table 4 below.

Table 4 nutrient analysis table of product obtained in comparative example 1

Index (I)	Content (wt.)
		Moisture content	3.9％
Crude protein	27.3％
		Crude fat	6.7％
Coarse ash content	4.5％

Comparative example 2

The comparative example provides a method for producing a granular energy protein feed by using threonine mother liquor, comprising the following steps:

(1) evaporating and concentrating the threonine mother liquor by a triple-effect evaporator, wherein the specific parameters are that the first-effect evaporation temperature is controlled at 88 ℃, the second-effect evaporation temperature is controlled at 70 ℃, the triple-effect evaporation temperature is controlled at 60 ℃, the content of concentrated dry matters is 50.5%, and amino acid crystals and other impurities are filtered and removed;

(2) adding 5% of quicklime into the threonine concentrated solution, and fully and uniformly mixing at 60 ℃;

(3) adding base material with particle diameter of 0.2mm into fluidized bed, feeding the prepared material liquid to fluidized bed nozzle via screw pump at 4L/h rate, and maintaining nozzle airflow pressure at 0.6MPa and 80m³The flow rate of inlet air is 140 ℃, the temperature of inlet air is 72 ℃, the tail gas is recycled by cyclone, and the recycled powder is reversely put into the fluidized bed. Discharging when the particles grow to about 2mm, cooling and packaging. This example was fluid granulationIn the process, a series of problems caused by excessive viscosity of the feed liquid do not occur. The main parameters of the fluidized granulation product are given in table 5 below.

TABLE 5 analysis table of nutrient components of the product obtained in comparative example 2

Index (I)	Content (%)
		Moisture content	6.2％
Crude protein	48.7％
		Crude fat	0％
Coarse ash content	19.7％

Examples of the experiments

For safety: according to the stacking standard of storage and transportation, the embodiment is placed in an environment with 70 ℃ and 10% humidity for a long time without spontaneous combustion, and compared with the spontaneous combustion risk of the dried sugar residues in the normal storage process, the invention can obviously improve the storage and transportation safety of the dried sugar residues.

For hygroscopicity: the nucleotide residues and glutamic acid mycoprotein produced by Tongliao plum blossom amino acid Co., Ltd are used as reference substances, the reference substances, and the products obtained in the examples and the comparative examples are put in a constant temperature and humidity chamber with the humidity of 25 ℃ and 70% overnight, and the moisture absorption rate is shown in Table 6 (moisture absorption rate ═ sample weight after moisture absorption-sample weight before moisture absorption)/sample weight before moisture absorption ═ 100%).

After the hygroscopicity test was conducted, the products of examples 1 to 3 were still in the form of dry granules, whereas the product of comparative example 1 was agglomerated into hard and large masses as a whole and had no fluidity, and the product of comparative example 2 was changed into a viscous liquid by absorbing a large amount of water, and could not be transported and used as a solid raw material.

TABLE 6 moisture absorption Rate profiles of the samples obtained in the examples and the corresponding control products

Sample (I)	Rate of moisture absorption
		Example 1	9.1％
Example 2	8.9％
		Example 3	10.3％
Comparative example 1	17.5％
		Comparative example 2	45.8％
Nucleotide residues (control)	9.9％
		Glutamic acid mycoprotein (control)	6.7％

The results show that the product obtained by the invention has good safety and the hygroscopicity is close to that of products such as nucleotide residues, glutamic acid mycoprotein and the like which are fermentation byproducts matured for feed production. From the aspect of product components, the product obtained by the invention has the characteristics of high protein, high fat and low ash content, so that under the era background that the prices of corn and bean pulp are gradually increased, the invention not only can bring certain benefits for corn deep processing and amino acid fermentation enterprises and reduce the environmental protection cost, but also can make an important contribution to feed cultivation enterprises to relieve the cost pressure.

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. A method of producing feed, comprising:

wherein the dry matter content of the amino acid mother liquor is 40-55 wt%;

2. the method of claim 1, wherein the amino acid fermentation mother liquor is obtained by fermenting escherichia coli as an engineering bacterium or corynebacterium glutamicum, and is preferably lysine fermentation mother liquor or threonine fermentation mother liquor.

3. The method as claimed in claim 1 or 2, characterized in that when the dry matter content of the amino acid fermentation mother liquor is lower than 40 wt%, the amino acid fermentation mother liquor is subjected to evaporation concentration by using a triple-effect evaporator, and the setting conditions of the triple-effect evaporator are that the first effect is 85-90 ℃, the second effect is 65-70 ℃ and the third effect is 55-60 ℃.

4. A method according to any one of claims 1-3, characterized in that the dry bagasse is obtained as follows:

5. The method according to any one of claims 1 to 4, wherein in the dry bagasse, 30-40 wt% crude protein, 20-30 wt% crude fat, 0.5-2 wt% ash, and 24.2-28.5MJ/kg total energy.

6. The method according to any one of claims 1-5, comprising:

7. The method of any one of claims 1 to 6, wherein the mixing is carried out at 55 to 65 ℃ and a pH of 5.0 to 8.0.

8. Method according to any of claims 1-7, characterized in that the method comprises the steps of:

mixing the amino acid fermentation mother liquor and the wet sugar residues, and then carrying out spray drying on the feed liquid, wherein the air flow pressure of a nozzle is set to be 0.3-0.5Mpa, the air inlet temperature is 110-130 ℃, the air outlet temperature is 65-75 ℃, and the water content of the product is controlled to be 4-6 wt%;

or, the method comprises the steps of:

mixing the amino acid fermentation mother liquor and the dry sugar residues, drying the feed liquid by a fluidized bed drying technology, controlling the grain diameter of a bottom material to be 0.2-0.3mm, controlling the airflow pressure of a nozzle to be 0.5-0.6MPa, controlling the air inlet temperature to be 90-110 ℃, controlling the air outlet temperature to be 60-70 ℃, discharging after the grain diameter is increased to 2-3mm, and controlling the water content of a product to be 3-5 wt%;

or, the method comprises the steps of:

9. A feed, characterized in that it is prepared by the method of any one of claims 1 to 8;

10. Use of the method of any one of claims 1-8 in any one of: