CN113072650A - Preparation method of jackfruit polysaccharide - Google Patents
Preparation method of jackfruit polysaccharide Download PDFInfo
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- CN113072650A CN113072650A CN202110341729.5A CN202110341729A CN113072650A CN 113072650 A CN113072650 A CN 113072650A CN 202110341729 A CN202110341729 A CN 202110341729A CN 113072650 A CN113072650 A CN 113072650A
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- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
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Abstract
The invention relates to the technical field of medicines, and particularly relates to a preparation method of wild pineapple polysaccharide. The preparation method comprises the following steps: steaming and drying the wild pineapple fruits to obtain dried wild pineapple fruits; crushing dry wild pineapple fruits, and carrying out water extraction, concentration, drying and crushing on the dry fruit powder to obtain a wild pineapple water extract; dissolving the wild pineapple aqueous extract in water, centrifuging, and filtering the supernatant to obtain clear liquid; precipitating the clear liquid with ethanol, drying the precipitate, pulverizing, and sieving to obtain primary product; dissolving the primary product in water, filtering, performing enzymolysis by using neutral protease, inactivating enzyme, and ultrafiltering to obtain trapped fluid; concentrating the trapped solution, precipitating with ethanol again, drying, and pulverizing. The purity of the wild pineapple polysaccharide extracted by the method is over 90 percent, the biological activity of the wild pineapple polysaccharide is improved, and the blood sugar and blood fat reducing effects of the wild pineapple polysaccharide are enhanced.
Description
Technical Field
The invention relates to the technical field of medicines, and particularly relates to a preparation method of wild pineapple polysaccharide.
Background
The wild pineapple is the fruit of Acanthopanax trifoliatus of Pandanus. Because of its shape similar to pineapple, Lingnan is commonly called wild pineapple. The wild pineapple is widely distributed in Hainan, has rich resources, can be eaten as wild fruit in folk, has sweet pulp, but has rich vascular tissues in the pulp and poor taste, so the wild pineapple is not popular and is used for treating diseases and cough in the Hainan folk. The root, leaf, etc. of Acanthopanax trifoliatus can be used as medicine, and has effects of clearing heat and eliminating dampness, promoting diuresis and relieving exterior syndrome, activating qi-flowing and calming liver. Modern pharmacological studies show that the effective components of the jackfruit can regulate the lipid metabolism of diabetic mice, reduce fat accumulation in vivo and improve insulin resistance. The wild pineapples have a history of being eaten as wild fruits for a long time, no report of toxic and side effects generated after eating is found at present, the safety is high, and the development is very facilitated.
According to the clear Bing Hai county Bing: the dewdrop tree is a village workshop and the phyllostachys pubescens is a garden and is used as a boundary, namely a dewdrop garden, namely a dewdrop. The dewdrop trees are mostly used as windbreak forests and gardens, the fiber of the leaves is a good product, and the dewdrop trees can be used for weaving crafts such as caps, mats, food drawers and the like; floral, aromatic oil can be extracted; it is rich in fruit, rich in meat, and edible, contains amino acids and saccharides, and can be used for treating nephritis and edema.
The Pandanus armandi contains vitamins, abundant mineral elements and multiple nutritional ingredients, and at least contains 17 amino acids, wherein 7 amino acids are necessary for human body. Wherein the total sugar, serine and glutamic acid have high mass fractions, and vitamin C and potassium have high mass fractions.
Patent example 1 with publication number CN103784623A discloses a medicinal plant extract of wild pineapple and its effective part preparation method, which comprises placing fresh and mature wild pineapple in a container, adding 10-20 times of water, soaking for 2h, decocting for 3 times (2 h each time), filtering, and concentrating to dry to obtain medicinal plant extract. Dissolving the medicinal plant extract with water, separating with D-101 macroporous adsorbent resin, eluting with ethanol-water solutions of different concentrations to colorless, concentrating the eluate under reduced pressure to obtain water eluate and 70% ethanol eluate, concentrating, and spray drying to obtain the effective component. Patent example 1 with publication number CN111265600A discloses a preparation method of a wild pineapple extract: cleaning wild pineapple with water, drying, adding 3L of water per kg, and soaking overnight. Heating to boil, extracting for 1.5 hr, filtering, collecting extractive solution, extracting with water once, mixing extractive solutions, heating, and concentrating to obtain extract. However, the polysaccharide content obtained by the preparation method is lower.
Disclosure of Invention
In view of the above, the invention provides a preparation method of jackfruit polysaccharide. The method can greatly improve purity and activity of wild pineapple polysaccharide, and enhance blood sugar and blood lipid reducing effects of wild pineapple polysaccharide.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of wild pineapple polysaccharide, which comprises the following steps:
(1) steaming and drying the wild pineapple fruits to obtain dried wild pineapple fruits;
(2) crushing dry wild pineapple fruits, and carrying out water extraction, concentration, drying and crushing on the dry fruit powder to obtain a wild pineapple water extract;
(3) dissolving the wild pineapple aqueous extract in water, centrifuging, and filtering the supernatant to obtain clear liquid;
(4) precipitating the clear liquid with ethanol, drying the precipitate, pulverizing, and sieving to obtain primary product;
(5) dissolving the primary product in water, filtering, performing enzymolysis by using neutral protease, inactivating enzyme, and ultrafiltering to obtain trapped fluid;
(6) concentrating the trapped solution, precipitating with ethanol again, drying, and pulverizing.
Preferably, in the step (1), the number of times of steaming and drying is 2-3, and the conditions of one-time steaming and drying are as follows: steaming for 30-180 min, and drying for 30-120 min at 60-100 ℃.
Preferably, in step (1), the number of times of steaming and drying is 2, and the first time of steaming and drying is: steaming for 30-180 min, and drying for 30-120 min at 60-100 ℃; the second steaming and drying comprises the following steps: steaming for 30-120 min, and drying for 30-120 min at 60-100 ℃.
Preferably, in the step (2), the number of water extractions is 2-3, and the conditions of one water extraction are as follows: adding water 8-18 times the weight of the dry fruit powder, and extracting at 90-100 ℃ for 30-120 min.
Preferably, in step (2), the number of water extractions is 2, and the conditions of the first water extraction are as follows: adding water with the weight being 10-18 times that of the dry fruit powder, and extracting for 60-120 min at 90-100 ℃; the conditions of the second water extraction are as follows: adding water with the weight being 8-15 times of that of the dry fruit powder, and extracting for 30-90 min at 90-100 ℃.
Preferably, in the step (2), the concentrated solution is concentrated to a solid content of 40-60%, the dried solution is dried in vacuum at 50-80 ℃, and the crushed mesh number is 40-120 meshes.
Preferably, in the step (3), the amount of water required for dissolving is 5-15 times of the weight of the wild pineapple aqueous extract, the centrifugation is 8000-10000 r/min for 6-10 min, and the aperture of the filtration is 0.2-0.3 nm.
Preferably, in the step (3), the amount of water required for dissolving is 5-15 times of the weight of the wild pineapple aqueous extract, the centrifugation is 8000 revolutions per minute for 6-10 minutes, and the aperture of the filtration is 0.22 nm.
Preferably, in step (4), the alcohol precipitation is: adding 90-100% ethanol until the concentration of the solution alcohol reaches 60-80%, and centrifuging at 7000-9000 r/min for 6-10 min; the drying temperature is 50-80 ℃.
Preferably, in step (4), the alcohol precipitation is: adding 95% ethanol until the concentration of the solution alcohol reaches 70%, and centrifuging at 8000 rpm for 6-10 min.
Preferably, in the step (5), the amount of water required for dissolving is 5-15 times of the weight of the primary product; the enzymolysis concentration of the neutral protease is 40000-100000IU/kg, and the enzymolysis condition is enzymolysis for 30-60 min at 45-55 ℃; the enzyme deactivation conditions are as follows: inactivating the enzyme at 90-100 ℃ for 5-20 min.
Preferably, in step (5), the ultrafiltration membrane used in the ultrafiltration has a molecular weight cut-off of 8000 Da.
Preferably, in the step (6), the mixture is concentrated to a solid content of 40-60%; the alcohol precipitation is as follows: adding 90-100% ethanol until the concentration of the solution alcohol reaches 60-80%, stirring, standing for 60-180 min, and centrifuging at 7000-9000 r/min for 6-10 min.
Preferably, in step (6), the alcohol precipitation is: adding 95% ethanol until the concentration of the solution alcohol reaches 70%, stirring, standing for 60-180 min, and centrifuging at 8000 rpm for 6-10 min.
Preferably, in the step (6), the drying temperature is 40 to 80 ℃.
The invention provides a preparation method of wild pineapple polysaccharide. The preparation method comprises the following steps: steaming and drying the wild pineapple fruits to obtain dried wild pineapple fruits; crushing dry wild pineapple fruits, and carrying out water extraction, concentration, drying and crushing on the dry fruit powder to obtain a wild pineapple water extract; dissolving the wild pineapple aqueous extract in water, centrifuging, and filtering the supernatant to obtain clear liquid; precipitating the clear liquid with ethanol, drying the precipitate, pulverizing, and sieving to obtain primary product; dissolving the primary product in water, filtering, performing enzymolysis by using neutral protease, inactivating enzyme, and ultrafiltering to obtain trapped fluid; concentrating the trapped solution, precipitating with ethanol again, drying, and pulverizing. The invention has the technical effects that:
the extraction of the wild pineapple polysaccharide takes fresh and plump wild pineapple fruits as raw materials, and the wild pineapple polysaccharide has the purity of over 90 percent and exists in the wild pineapple fruits through processes of double steaming, crushing, secondary extraction, ethanol precipitation for impurity removal, enzymolysis and ultrafiltration for protein, salt and impurity removal, secondary concentration, ethanol precipitation for impurity removal, drying, crushing, sieving and the like.
Detailed Description
The invention discloses a preparation method of wild pineapple polysaccharide, and a person skilled in the art can appropriately improve process parameters by referring to the content. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The raw materials, reagents or instruments used in the preparation method of the wild pineapple polysaccharide provided by the invention can be purchased from the market.
The invention is further illustrated by the following examples:
example 1:
taking 80kg of wild pineapple fruits, cleaning, steaming for 30min, taking out, drying at 60 ℃ for 30min, steaming for 30min for the second time, taking out, drying at 60 ℃ for 30min, taking out to obtain 13.5kg of dried wild pineapple fruits, crushing to obtain 13.2kg of dry wild pineapple powder, adding 10 times of water, heating to 90 ℃, extracting for 60min, and filtering to obtain a filtrate I; adding 8 times of water into the filter residue, heating to above 90 deg.C, extracting for 30min, filtering to obtain filtrate II, mixing the filtrates I and II, concentrating until the solid content is 40%, vacuum drying at 50 deg.C, pulverizing, and sieving with 40 mesh sieve to obtain 3.23kg of wild pineapple extract.
Adding 5 times of water into the wild pineapple extract, stirring for dissolving, centrifuging at 8000 rpm for 6min, collecting the clear liquid, passing the solution through a 0.22nm membrane, adding 95% ethanol until the ethanol concentration of the solution reaches 70%, centrifuging at 8000 rpm for 6min, collecting the precipitate, washing the precipitate twice with absolute ethanol, drying at 50 deg.C, pulverizing, and sieving to obtain 1.13kg of crude wild pineapple polysaccharide.
Dissolving crude wild pineapple polysaccharide with 5 times of water, filtering, adding 40000 international unit specific activity neutral protease per kg, hydrolyzing at 45 deg.C for 30min, heating to 90 deg.C after enzymolysis, maintaining for 5min for enzyme inactivation, passing the enzymolysis solution through 8000Da ultrafiltration membrane to obtain trapped fluid 1.6kg, concentrating to solid content of 40%, adding 95% ethanol into the concentrated solution until the ethanol content of the solution reaches 70%, stirring thoroughly, standing for 60min, centrifuging at 8000 rpm for 6min, collecting precipitate, washing the precipitate with anhydrous ethanol twice, drying at 40 deg.C, pulverizing, and sieving to obtain 226.15g of wild pineapple polysaccharide.
Example 2:
taking 80kg of wild pineapple fruits, cleaning, steaming for 105min, taking out, drying at 80 ℃ for 75min, steaming for 75min for the second time, taking out, drying at 80 ℃ for 75min, taking out to obtain 13.8kg of dried wild pineapple fruits, crushing, adding 14 times of water, heating to 95 ℃, extracting for 90min, and filtering to obtain a filtrate I; adding 11.5 times of water into the filter residue, heating to above 95 deg.C, extracting for 60min, filtering to obtain filtrate II, mixing the filtrates I and II, concentrating until the solid content is 50%, vacuum drying at 65 deg.C, pulverizing, and sieving with 80 mesh sieve to obtain 3.45kg of wild pineapple extract.
Adding 10 times of water into the wild pineapple extract, stirring for dissolving, centrifuging at 8000 rpm for 8min, collecting the clear liquid, passing the solution through a 0.22nm membrane, adding 95% ethanol until the ethanol concentration of the solution reaches 70%, centrifuging at 8000 rpm for 8min, collecting the precipitate, washing the precipitate twice with absolute ethanol, drying at 65 deg.C, pulverizing, and sieving to obtain 1.21kg of crude wild pineapple polysaccharide.
Dissolving crude wild pineapple polysaccharide in 10 times of water, filtering, adding 70000 international unit specific activity neutral protease per kg, hydrolyzing at 50 deg.C for 45min, heating to 95 deg.C after enzymolysis, and maintaining for 12.5min for enzyme inactivation. Passing the enzymolysis solution through 8000Da ultrafiltration membrane, collecting the trapped solution, concentrating to solid content of 50%, adding 95% ethanol until the ethanol content of the solution reaches 70%, stirring thoroughly, standing for 120min, centrifuging at 8000 rpm for 8min, collecting precipitate, washing the precipitate with anhydrous ethanol twice, drying at 60 deg.C, pulverizing, and sieving to obtain 238.11g wild pineapple polysaccharide.
Example 3:
taking 80kg of wild pineapple fruits, cleaning, steaming for 180min, taking out, drying at 100 ℃ for 120min, steaming for 120min for the second time, taking out, drying at 100 ℃ for 120min, taking out to obtain 13.0kg of dried wild pineapple fruits, crushing, adding 18 times of water, heating to 100 ℃, extracting for 120min, and filtering to obtain a filtrate I; adding 15 times of water into filter residue, heating to 100 deg.C, extracting for 90min, filtering to obtain filtrate (II), mixing filtrates (I) and (II), concentrating until solid is 60%, vacuum drying at 80 deg.C, pulverizing, and sieving with 120 mesh sieve to obtain wild pineapple extract 3.48 kg;
adding 15 times of water into the wild pineapple extract, stirring for dissolving, centrifuging at 8000 rpm for 10min, collecting clear liquid, passing the solution through a 0.22nm membrane, adding 95% ethanol until the ethanol concentration of the solution reaches 70%, centrifuging at 8000 rpm for 10min, collecting precipitate, washing the precipitate twice with absolute ethanol, drying at 80 deg.C, pulverizing, and sieving to obtain 1.20kg of crude wild pineapple polysaccharide.
Dissolving crude wild pineapple polysaccharide with 15 times of water, filtering, adding 100000 international unit specific activity neutral protease per kg, hydrolyzing at 55 deg.C for 60min, heating to 100 deg.C after enzymolysis, maintaining for 20min for enzyme inactivation, passing the enzymolysis solution through 8000Da ultrafiltration membrane, collecting the trapped fluid, concentrating until the solid content is 60%, adding 95% ethanol until the ethanol content of the solution reaches 70%, stirring, standing for 180min, centrifuging at 8000 rpm for 10min, collecting precipitate, washing the precipitate with anhydrous ethanol twice, drying at 80 deg.C, pulverizing, and sieving to obtain 240.20g of wild pineapple polysaccharide.
Test example 1: detection test
Polysaccharide detection method (phenol-sulfuric acid method): and (3) determining the content of the polysaccharide by using a sulfuric acid-phenol color development method by taking D-anhydrous glucose as a reference, wherein a standard curve equation is that y is 7.66812x +0.00000(r is 0.99926), wherein x is the mass concentration (mg/mL) of the D-anhydrous glucose and y is absorbance, and calculating the content of the polysaccharide according to a regression equation after measuring the absorbance.
Table 1: product detection
| Serial number | Product(s) | Polysaccharide content |
| 1 | Example 1 | 92.3％ |
| 2 | Example 2 | 93.6％ |
| 3 | Example 3 | 93.0％ |
| 4 | CN103784623A sample | 62.8％ |
| 5 | CN111265600A sample | 26.1％ |
Test example 2: ananas comosus polysaccharide blood sugar lowering test
1. Material
70 healthy, male and clean rats with the weight of 205-: SCXK (Liao) 2019-.
2. Main instrument equipment
Glucometer, a product of roche germany;
a full-automatic biochemical analyzer, a product of Hitachi, Japan;
ELISA detection kit, Nanjing Senega Biotech limited.
3. Experimental methods
3.1 sample preparation
The wild pineapple polysaccharide prepared by the invention.
3.2 diabetes model establishment
After the rats are fasted for 12 hours without water prohibition, injecting the streptozotocin freshly prepared by citric acid-disodium hydrogen phosphate buffer solution with the pH value of 4.4 into the abdominal cavity for 50mg/kg, and after 24 hours of molding, detecting urine sugar by using urine sugar test paper, collecting blood by tail vein blood, detecting fasting blood sugar, and detecting strong urine sugar positivity, wherein the fasting blood sugar is more than or equal to 16.5mmol/L, and the molding success is determined.
3.3 experiment
The modeling was successful, and rats were randomly divided into irbesartan group, sample low dose group, medium dose group, high dose group, CN103784623A sample group, and CN111265600A sample group, 10 each.
The model group was given saline; irbesartan group is given with 17.5mg/kg irbesartan; 30mg/kg of wild pineapple polysaccharide is given to a low-dose group; the middle dose group is administered with 60mg/kg of jackfruit polysaccharide; the high-dose group is administered with 120mg/kg of jackfruit polysaccharide; the CN103784623A sample group gives 120mg/kg of CN103784623A samples; the CN111265600A sample group gave 120mg/kg of CN111265600A sample. Each group of rats was gavaged 1 time daily for 7 consecutive days.
3.4FBG, BUN level detection
60min after the last gastric lavage, cutting the tail and taking blood. Detecting Fasting Plasma Glucose (FPG) levels using a glucometer; the urea nitrogen (BUN) level was determined using a fully automatic biochemical analyzer.
4. Results and discussion
Researches show that compared with a model group, FBG and BUN levels of an irbesartan group, a sample low-dose group, a sample medium-dose group and a sample high-dose group are obviously reduced and have obvious differences. Wherein the level of FBG of the high agent group at 9.45mmol/L, BUN at 9.23mmol/L is obviously reduced, and the difference exists.
The levels of FBG and BUN in the CN103784623A sample group and CN111265600A sample group were not significantly reduced compared to the model group.
Table 2: effect of Jacobian polysaccharides on rat FBG and BUN levels
Note: as can be seen in the table, FBG and BUN levels in irbesartan, the dosage in sample and the high dosage groups were significantly reduced compared to the model group (p <0.01), and FBG and BUN levels in the low dosage sample and CN103784623A sample groups were significantly reduced compared to the model group (p < 0.05). The FBG and BUN levels of the CN111265600A sample group are not obviously reduced compared with the model group.
Test example 3: jackfruit polysaccharide with blood fat reducing effect
1. Experimental medicine and equipment
Statin for the positive control group: "simvastatin";
sample preparation: the product of the invention is wild pineapple polysaccharide;
the high-fat feed comprises 20% of lard, 1% of cholesterol, 0.2% of bile salt, 18% of sugar and a high-nutrition basic feed.
The apparatus used was a 16# rat gavage apparatus with an accuracy of 0.1g platform scale and 1mL syringe capacity.
2. Laboratory animal
80 healthy male white rats of 4 weeks of age were randomly divided into 8 groups.
3. Experimental methods
80 rats were randomly divided into 8 groups. The test results were divided into a control group, a high-fat model group, a simvastatin group, a wildpineapple polysaccharide high-dose group, a medium-dose group, a low-dose group, a CN103784623A sample group and a CN111265600A sample group. A high-fat model group, a simvastatin group, a wild pineapple polysaccharide high-dose group, a medium-dose group, a low-dose group, a CN103784623A sample group and a CN111265600A sample group are fed with high-fat feed for 4 weeks by adopting a high-fat diet method, so that the model building can be successful in time, and blood sampling assay is carried out every other week until the model building of a high-fat rat is successful.
The control group was given physiological saline in the high-fat model, the simvastatin group was given 20mg/kg simvastatin, the high-dose group was given 160mg/kg crabapple polysaccharide, the middle-dose group was given 80mg/kg crabapple polysaccharide, the low-dose group was given 40mg/kg crabapple polysaccharide, the CN103784623A sample group was given 160mg/kg sample, and the CN111265600A sample group was given 160mg/kg sample.
Experiments were performed using a high-fat diet method, a control analysis method, an abdominal gavage method, and an abdominal blood sampling method. The white rat is selected as the experimental object, because the white rat not only has better effect than a mouse in the aspect of absorbing the medicament reagent without blood vessel injection, but also can smoothly take 3-4mL of whole blood in blood collection.
Table 3: four items of blood fat
Note: as can be seen from the table, the blood lipid levels of the low dose group, the medium dose group and the high dose group of the samples were significantly reduced compared with the high fat control group (p <0.01), and the blood lipid levels of the simvastatin group were significantly reduced compared with the high fat control group (p < 0.05). The four blood lipids of the CN103784623A sample group and the CN111265600A sample group did not significantly decrease compared to the high lipid control group.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The preparation method of the wild pineapple polysaccharide is characterized by comprising the following steps:
(1) steaming and drying the wild pineapple fruits to obtain dried wild pineapple fruits;
(2) crushing dry wild pineapple fruits, and carrying out water extraction, concentration, drying and crushing on the dry fruit powder to obtain a wild pineapple water extract;
(3) dissolving the wild pineapple aqueous extract in water, centrifuging, and filtering the supernatant to obtain clear liquid;
(4) precipitating the clear liquid with ethanol, drying the precipitate, pulverizing, and sieving to obtain primary product;
(5) dissolving the primary product in water, filtering, performing enzymolysis by using neutral protease, inactivating enzyme, and ultrafiltering to obtain trapped fluid;
(6) concentrating the trapped solution, precipitating with ethanol again, drying, and pulverizing.
2. The preparation method according to claim 1, wherein in the step (1), the number of times of steaming and drying is 2-3, and the conditions of one-time steaming and drying are as follows: steaming for 30-180 min, and drying for 30-120 min at 60-100 ℃.
3. The preparation method according to claim 1, wherein in the step (2), the number of water extractions is 2-3, and the conditions of one water extraction are as follows: adding water 8-18 times the weight of the dry fruit powder, and extracting at 90-100 ℃ for 30-120 min.
4. The preparation method according to claim 1, wherein in the step (2), the concentration is carried out until the solid content is 40-60%, the drying is carried out at 50-80 ℃ under vacuum, and the crushed mesh number is 40-120 meshes.
5. The preparation method according to claim 1, wherein in the step (3), the amount of water required for dissolution is 5-15 times of the weight of the wild pineapple aqueous extract, the centrifugation is 8000-10000 r/min for 6-10 min, and the pore size of the filtration is 0.2-0.3 nm.
6. The preparation method according to claim 1, wherein in the step (4), the alcohol precipitation is: adding 90-100% ethanol until the concentration of the solution alcohol reaches 60-80%, and centrifuging at 7000-9000 r/min for 6-10 min; the drying temperature is 50-80 ℃.
7. The method according to claim 1, wherein in the step (5), the amount of water required for dissolution is 5 to 15 times the weight of the primary product; the enzymolysis concentration of the neutral protease is 40000-100000IU/kg, and the enzymolysis condition is enzymolysis for 30-60 min at 45-55 ℃; the enzyme deactivation conditions are as follows: inactivating the enzyme at 90-100 ℃ for 5-20 min.
8. The method according to claim 1, wherein the ultrafiltration membrane used in the step (5) has a molecular weight cut-off of 8000 Da.
9. The method according to claim 1, wherein in the step (6), the concentration is carried out until the solid content is 40-60%; the alcohol precipitation is as follows: adding 90-100% ethanol until the concentration of the solution alcohol reaches 60-80%, stirring, standing for 60-180 min, and centrifuging at 7000-9000 r/min for 6-10 min.
10. The production method according to any one of claims 1 to 9, wherein in the step (6), the temperature of the drying is 40 to 80 ℃.
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| CN202110341729.5A CN113072650B (en) | 2021-03-30 | 2021-03-30 | Preparation method of jackfruit polysaccharide |
| PCT/CN2021/087683 WO2022205518A1 (en) | 2021-03-30 | 2021-04-16 | Method for preparing pandanus tectorius polysaccharide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116003647A (en) * | 2023-01-03 | 2023-04-25 | 广东海洋大学 | Jackfruit peel polysaccharide, preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103784623A (en) * | 2012-11-01 | 2014-05-14 | 中国医学科学院药用植物研究所 | Preparation method of medicinal plant extract for reducing blood sugar and application in pharmacy and health food thereof |
| CN111265600A (en) * | 2020-03-18 | 2020-06-12 | 海南医学院 | Application of jackfruit extract in preparing weight-reducing preparation |
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| CN103254325B (en) * | 2013-05-31 | 2016-03-02 | 华南理工大学 | A kind of preparation method of Herba Abri polysaccharide |
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| CN103784623A (en) * | 2012-11-01 | 2014-05-14 | 中国医学科学院药用植物研究所 | Preparation method of medicinal plant extract for reducing blood sugar and application in pharmacy and health food thereof |
| CN111265600A (en) * | 2020-03-18 | 2020-06-12 | 海南医学院 | Application of jackfruit extract in preparing weight-reducing preparation |
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| CHONGMING WU ET AL: "The caffeoylquinic acid-rich Pandanus tectorius fruit extract increases insulin sensitivity and regulates hepatic glucose and lipid metabolism in diabetic db/db mice", 《THE JOURNAL OF NUTRITION BIOCHEMISTRY》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116003647A (en) * | 2023-01-03 | 2023-04-25 | 广东海洋大学 | Jackfruit peel polysaccharide, preparation method and application |
| CN116003647B (en) * | 2023-01-03 | 2024-05-28 | 广东海洋大学 | Jackfruit peel polysaccharide, preparation method and application |
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| CN113072650B (en) | 2022-01-21 |
| WO2022205518A1 (en) | 2022-10-06 |
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