CN113072408A - Special biofertilizer for cucumber seedlings and preparation method thereof - Google Patents
Special biofertilizer for cucumber seedlings and preparation method thereof Download PDFInfo
- Publication number
- CN113072408A CN113072408A CN202110449327.7A CN202110449327A CN113072408A CN 113072408 A CN113072408 A CN 113072408A CN 202110449327 A CN202110449327 A CN 202110449327A CN 113072408 A CN113072408 A CN 113072408A
- Authority
- CN
- China
- Prior art keywords
- parts
- agent
- sweet potato
- compound
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a special biofertilizer for cucumber seedlings, which is prepared from the following raw materials in parts by weight: 4-12 parts of attapulgite, 4-15 parts of rapeseed cakes, 4-23 parts of a corn cob fermentation storage compound agent, 2-26 parts of a corn straw decomposition agent, 3-19 parts of a sweet potato flour compound agent and 1-5 parts of a compound microbial inoculum. The special biofertilizer for cucumber seedlings can create a good rhizosphere ecological environment, improve the physical and chemical properties of soil, improve the fertilizer supply capacity of the soil and fully utilize environmental resources, so that the taste and the yield of cucumbers are improved, and the biofertilizer has a good popularization and application prospect.
Description
Technical Field
The invention belongs to the technical field of agricultural fertilizers, and particularly relates to a special biological fertilizer for cucumber seedlings and a preparation method thereof.
Background
The biological fertilizer has the advantages of no pollution, no residue, soil fertility and crop quality improvement, so the biological fertilizer is a main fertilizer for pollution-free agricultural products and green food. Compared with chemical fertilizers, the main functions of the biological fertilizer are to improve the microbial structure composition of soil and improve the nutrient condition of the soil, and the main function of the chemical fertilizer is to provide nutrients. Compared with organic fertilizers, the biological fertilizer has the advantages of improving the absorption rate of crops to nutrient substances, restoring soil health and regulating the self functionality of the crops.
The biological fertilizer is used for increasing the yield by the action of microorganisms. The biological fertilizer does not contain nutrient elements required by the growth of crops, but improves the nutrient conditions of the crops through the life activities of microorganisms, such as fixing nitrogen in the air, participating in the conversion of nutrients, promoting the absorption of the crops to the nutrients, secreting various hormones to stimulate the root system development of the crops, inhibiting the activities of harmful microorganisms and the like, so as to achieve the purpose of promoting the growth of the crops. Microbial activity requires the expenditure of energy. Organic matters are the main energy sources of microorganisms, and the decomposition of the organic matters can also supply nutrients to the microorganisms.
Because the growth and development of crops need various nutrient elements, the microbial fertilizer with a single strain and a single function can not meet the requirements of modern agricultural development. The biological fertilizer can organically combine enzyme, organic matters and microorganisms into a whole, embodies the quick action, the long-acting property, the synergy and the disease resistance of the fertilizer, reduces the application amount of the fertilizer, improves the utilization rate of the fertilizer by more than 30 percent, and has multiple effects of loosening soil, nourishing roots, fertilizing fields, controlling pests, increasing yield and the like.
The cucumber is a high-yield vegetable, and has the advantages of fast growth, more fruits, long cucumber-bearing period and high nutrient consumption. The biological fertilizer is also a shallow root crop, the root quantity is less, the distribution is shallow in soil, the water and nutrients below a root layer are difficult to utilize, the fertility of the root system is weak, if the fertilizer is applied too much or the concentration of the fertilizer is too high at one time, the root burning phenomenon can occur, the biological fertilizer does not directly provide nutrient substances (including nitrogen, phosphorus, potassium and various mineral elements) absorbed by the crop, but the biological fertilizer is made and assisted by the active activity of a large number of active microorganisms in the soil to absorb the nutrient substances or generate growth hormone to stimulate the growth of the crop, and the slow release effect is achieved, so that the biological fertilizer can continuously provide the nutrient substances required by the cucumber.
The cucumber cultivated by the existing biological fertilizer has poor taste, low sweetness, not strong enough cucumber seedling growth and low yield.
Disclosure of Invention
In view of the above, the invention aims to provide a biofertilizer special for cucumber seedlings, which can create a good rhizosphere ecological environment, improve the physical and chemical properties of soil, improve the fertilizer supply capacity of the soil and fully utilize environmental resources, so that the taste and the yield of cucumbers are improved, and the biofertilizer has a good popularization and application prospect.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
a biofertilizer special for cucumber seedlings is prepared from the following raw materials in parts by weight: 4-12 parts of attapulgite, 4-15 parts of rapeseed cakes, 4-23 parts of a corn cob fermentation storage compound agent, 2-26 parts of a corn straw decomposition agent, 3-19 parts of a sweet potato flour compound agent and 1-5 parts of a compound microbial inoculum.
Further, the corn cob fermentation storage compound agent is obtained by mixing the compound microbial inoculum and crushed corn cobs according to the weight ratio of 1:25, adjusting the humidity to 50-70%, and fermenting and storing for 15-20 days at the temperature of 35-45 ℃.
Further, the corn straw decomposing inoculant is obtained by mixing the composite inoculant and crushed corn straws in a weight ratio of 1:25, adjusting the humidity to 50-70%, and fermenting and storing for 25-30 days at the temperature of 55-65 ℃.
Further, the sweet potato powder compound agent is prepared by mixing medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the material-water ratio is 4: 1, uniformly mixing the mixture with water at the temperature of 60-75 ℃, and naturally cooling the mixture to obtain the product.
Further, the compound microbial inoculum comprises the following raw materials in parts by weight: 3-7 parts of pseudomonas, 2-7 parts of streptomyces microflavus, 3-11 parts of brevibacillus laterosporus, 3-13 parts of bacillus mucilaginosus, 1-7 parts of phosphate-solubilizing potassium-solubilizing bacteria, 2-15 parts of bacillus subtilis, 1-8 parts of candida tropicalis, 3-9 parts of bacillus licheniformis, 2-9 parts of bacillus megaterium, 1-9 parts of cellulose streptomyces and 2-8 parts of rhodopseudomonas palustris; the strain also comprises sucrose or fructo-oligosaccharide, and the weight ratio of the sucrose or fructo-oligosaccharide to the mixed strain is 5: 1.
The invention also provides a preparation method of the special biofertilizer for cucumber seedlings, which comprises the following steps:
1) preparation of composite microbial inoculum
Taking commercially available pseudomonas, streptomyces microflavus, brevibacillus laterosporus, bacillus mucilaginosus, phosphorus and potassium solubilizing bacteria, bacillus subtilis, candida tropicalis, bacillus licheniformis, bacillus megaterium, streptomyces cellulosae and rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and then mixing the composite bacteria with sucrose or fructo-oligosaccharide according to a weight ratio of 1:5 to obtain a composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and crushed corncobs are mixed according to the weight ratio of 1:25, the humidity is adjusted to 50-70%, and the corncobs are fermented and stored for 15-20 days at the temperature of 35-45 ℃ to obtain a corncob fermented and stored compound agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and crushed corn straws are mixed according to the weight ratio of 1:25, the humidity is adjusted to 50-70%, and the corn straws are fermented and stored for 25-30 days at the temperature of 55-65 ℃ to obtain a corn straw fermented and stored compound agent;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing water at the temperature of 60-75 ℃, and naturally cooling to obtain a sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Compared with the prior art, the special biological fertilizer for cucumber seedlings has the following advantages:
compared with the prior art: the biological fertilizer for promoting the growth of the cucumbers can improve the physical and chemical properties of soil and the fertilizer supply capacity of the soil, has high fertilizer utilization rate, can obviously promote the growth of cucumber seedlings and the stress resistance of the cucumbers, greatly improves the yield of the cucumbers, and can also greatly improve the quality of the cucumbers due to the synergistic effect of the components in the fertilizer.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The present invention will be described in detail with reference to examples.
Example 1
A biofertilizer special for cucumber seedlings is prepared from the following raw materials in parts by weight: 4 parts of attapulgite, 4 parts of rapeseed cakes, 4 parts of a corncob fermentation storage compound agent, 2 parts of a corn straw decomposition agent, 3 parts of a sweet potato powder compound agent and 1 part of a compound microbial inoculum.
The preparation method of the special biofertilizer for cucumber seedlings comprises the following steps:
1) preparation of composite microbial inoculum
Taking 3 parts of commercially available pseudomonas, 3 parts of streptomyces microflavus, 3 parts of brevibacillus laterosporus, 5 parts of bacillus mucilaginosus, 4 parts of phosphate-solubilizing potassium-solubilizing bacteria, 2 parts of bacillus subtilis, 3 parts of candida tropicalis, 3 parts of bacillus licheniformis, 2 parts of bacillus megaterium, 2 parts of cellulose streptomyces and 3 parts of rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and then mixing the composite bacteria with sucrose fructo-oligosaccharide according to a weight ratio of 1:5 to obtain a composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) are mixed with the crushed corncobs according to the weight ratio of 1:25, the humidity is adjusted to 65%, and the corncobs are fermented and stored for 15 days at the temperature of 40 ℃ to obtain a corncob yeast storage compounding agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) are mixed with the crushed corn straws according to the proportion of 1:25, the humidity is adjusted to 70%, and the corn straw fermented material mixed agent is obtained after fermentation storage for 25 days at the temperature of 60 ℃;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing the components at the water temperature of 65 ℃, and naturally cooling to obtain the sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Example 2
A biofertilizer special for cucumber seedlings is prepared from the following raw materials in parts by weight: 7 parts of attapulgite, 8 parts of rapeseed cakes, 7 parts of a corn cob fermentation storage compound agent, 5 parts of a corn straw decomposition agent, 8 parts of a sweet potato powder compound agent and 3 parts of a compound microbial inoculum.
The preparation method of the special biofertilizer for cucumber seedlings comprises the following steps:
1) preparation of composite microbial inoculum
Taking 5 parts of commercially available pseudomonas, 4 parts of streptomyces microflavus, 8 parts of brevibacillus laterosporus, 7 parts of bacillus mucilaginosus, 5 parts of phosphate-solubilizing potassium-solubilizing bacteria, 8 parts of bacillus subtilis, 4 parts of candida tropicalis, 6 parts of bacillus licheniformis, 5 parts of bacillus megaterium, 5 parts of cellulose streptomyces and 4 parts of rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and mixing the composite bacteria and fructo-oligosaccharide according to a weight ratio of 1:5 to obtain a composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and crushed corncobs are mixed according to the weight ratio of 1:25, the humidity is adjusted to 60%, and the corncobs are fermented and stored for 18 days at the temperature of 45 ℃ to obtain a corncob yeast storage compounding agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) are mixed with the crushed corn straws according to the proportion of 1:25, the humidity is adjusted to 60%, and the mixture is fermented and stored for 25 days at the temperature of 60 ℃ to obtain a corn straw fermented product mixture;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing at 70 ℃, and naturally cooling to obtain a sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Example 3
A biofertilizer special for cucumber seedlings is prepared from the following raw materials in parts by weight: 6 parts of attapulgite, 9 parts of rapeseed cakes, 20 parts of a corn cob fermentation storage compound agent, 22 parts of a corn straw decomposition agent, 13 parts of a sweet potato powder compound agent and 5 parts of a compound microbial inoculum.
The preparation method of the special biofertilizer for cucumber seedlings comprises the following steps:
1) preparation of composite microbial inoculum
Taking 7 parts of commercially available pseudomonas, 6 parts of streptomyces microflavus, 11 parts of brevibacillus laterosporus, 10 parts of bacillus mucilaginosus, 7 parts of phosphate-solubilizing potassium-solubilizing bacteria, 11 parts of bacillus subtilis, 8 parts of candida tropicalis, 9 parts of bacillus licheniformis, 8 parts of bacillus megaterium, 9 parts of cellulose streptomyces and 8 parts of rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and mixing the composite bacteria and sucrose according to a weight ratio of 1:5 to obtain a composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and the crushed corncobs are mixed according to the weight ratio of 1:25, the humidity is adjusted to 70%, and the corncobs are fermented and stored for 20 days at the temperature of 40 ℃ to obtain a corncob yeast storage compounding agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) are mixed with the crushed corn straws according to the proportion of 1:25, the humidity is adjusted to 70%, and the corn straw fermented material mixed agent is obtained after fermentation storage for 28 days at the temperature of 65 ℃;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing water at 75 ℃, and naturally cooling to obtain a sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Example 4
A biofertilizer special for cucumber seedlings is prepared from the following raw materials in parts by weight: 11 parts of attapulgite, 10 parts of rapeseed cakes, 6 parts of a corn cob fermentation storage compound agent, 15 parts of a corn straw decomposition agent, 7 parts of a sweet potato powder compound agent and 1 part of a compound microbial inoculum.
The preparation method of the special biofertilizer for cucumber seedlings comprises the following steps:
1) preparation of composite microbial inoculum
Taking 4 parts of commercially available pseudomonas, 7 parts of streptomyces microflavus, 8 parts of brevibacillus laterosporus, 13 parts of bacillus mucilaginosus, 6 parts of phosphate-solubilizing potassium-solubilizing bacteria, 15 parts of bacillus subtilis, 8 parts of candida tropicalis, 5 parts of bacillus licheniformis, 7 parts of bacillus megaterium, 6 parts of cellulose streptomyces and 6 parts of rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and then mixing the composite bacteria and fructo-oligosaccharide according to a weight ratio of 1:5 to obtain a composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and the crushed corncobs are mixed according to the weight ratio of 1:25, the humidity is adjusted to 55%, and the corncobs are fermented and stored for 20 days at the temperature of 45 ℃ to obtain a corncob yeast storage compounding agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) are mixed with the crushed corn straws according to the proportion of 1:25, the humidity is adjusted to 65%, and the corn straw fermented material mixed agent is obtained after fermentation storage for 30 days at the temperature of 65 ℃;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing at 70 ℃, and naturally cooling to obtain a sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Example 5
A biofertilizer special for cucumber seedlings is prepared from the following raw materials in parts by weight: 12 parts of attapulgite, 15 parts of rapeseed cakes, 4 parts of a corn cob fermentation storage compound agent, 5 parts of a corn straw decomposition agent, 3 parts of a sweet potato powder compound agent and 3 parts of a compound microbial inoculum.
The preparation method of the special biofertilizer for cucumber seedlings comprises the following steps:
1) preparation of composite microbial inoculum
Taking 3 parts of commercially available pseudomonas, 7 parts of streptomyces microflavus, 11 parts of brevibacillus laterosporus, 5 parts of bacillus mucilaginosus, 3 parts of phosphate-solubilizing potassium-solubilizing bacteria, 15 parts of bacillus subtilis, 4 parts of candida tropicalis, 9 parts of bacillus licheniformis, 2 parts of bacillus megaterium, 9 parts of cellulose streptomyces and 4 parts of rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and then mixing the composite bacteria with sucrose or fructo-oligosaccharide according to a weight ratio of 1:5 to obtain a composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and the crushed corncobs are mixed according to the weight ratio of 1:25, the humidity is adjusted to 65%, and the corncobs are fermented and stored for 20 days at the temperature of 38 ℃ to obtain a corncob yeast storage compounding agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) are mixed with the crushed corn straws according to the proportion of 1:25, the humidity is adjusted to 50%, and the corn straw fermented material mixed agent is obtained after fermentation storage for 30 days at the temperature of 65 ℃;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing water at 75 ℃, and naturally cooling to obtain a sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Example 6
A biofertilizer special for cucumber seedlings is prepared from the following raw materials in parts by weight: 9 parts of attapulgite, 5 parts of rapeseed cakes, 17 parts of a corn cob fermentation storage compound agent, 5 parts of a corn straw decomposition agent, 4 parts of a sweet potato powder compound agent and 4 parts of a compound microbial inoculum.
The preparation method of the special biofertilizer for cucumber seedlings comprises the following steps:
1) preparation of composite microbial inoculum
Taking 7 parts of commercially available pseudomonas, 4 parts of streptomyces microflavus, 6 parts of brevibacillus laterosporus, 13 parts of bacillus mucilaginosus, 3 parts of phosphate-solubilizing potassium-solubilizing bacteria, 12 parts of bacillus subtilis, 6 parts of candida tropicalis, 7 parts of bacillus licheniformis, 7 parts of bacillus megaterium, 6 parts of cellulose streptomyces and 3 parts of rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and then mixing the composite bacteria with sucrose or fructo-oligosaccharide according to a weight ratio of 1:5 to obtain a composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and the crushed corncobs are mixed according to the weight ratio of 1:25, the humidity is adjusted to 58%, and the corncobs are fermented and stored for 16 days at the temperature of 42 ℃ to obtain a corncob yeast storage compounding agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and crushed corn straws are mixed according to the proportion of 1:25, the humidity is adjusted to 68%, and the temperature is 59 ℃ for fermentation and storage for 27 days, so as to obtain a corn straw fermented product mixture;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing the components at the water temperature of 73 ℃, and naturally cooling to obtain the sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Example 7
A biofertilizer special for cucumber seedlings is prepared from the following raw materials in parts by weight: 12 parts of attapulgite, 15 parts of rapeseed cakes, 11 parts of a corn cob fermentation storage compound agent, 15 parts of a corn straw decomposition agent, 9 parts of a sweet potato powder compound agent and 1 part of a compound microbial inoculum.
The preparation method of the special biofertilizer for cucumber seedlings comprises the following steps:
1) preparation of composite microbial inoculum
Taking 6 parts of commercially available pseudomonas, 4 parts of streptomyces microflavus, 9 parts of brevibacillus laterosporus, 10 parts of bacillus mucilaginosus, 5 parts of phosphate-solubilizing potassium-solubilizing bacteria, 13 parts of bacillus subtilis, 4 parts of candida tropicalis, 9 parts of bacillus licheniformis, 7 parts of bacillus megaterium, 3 parts of cellulose streptomyces and 5 parts of rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and then mixing the composite bacteria with sucrose or fructo-oligosaccharide according to a weight ratio of 1:5 to obtain a composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and the crushed corncobs are mixed according to the weight ratio of 1:25, the humidity is adjusted to 70%, and the corncobs are fermented and stored for 20 days at the temperature of 40 ℃ to obtain a corncob yeast storage compounding agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) are mixed with the crushed corn straws according to the proportion of 1:25, the humidity is adjusted to 65%, and the corn straw fermented material mixed agent is obtained after fermentation storage for 25 days at the temperature of 60 ℃;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing at 70 ℃, and naturally cooling to obtain a sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Example 8
A biofertilizer special for cucumber seedlings is prepared from the following raw materials in parts by weight: 5 parts of attapulgite, 6 parts of rapeseed cakes, 17 parts of a corn cob fermentation storage compound agent, 15 parts of a corn straw decomposition agent, 6 parts of a sweet potato powder compound agent and 3 parts of a compound microbial inoculum.
The preparation method of the special biofertilizer for cucumber seedlings comprises the following steps:
1) preparation of composite microbial inoculum
Taking 7 parts of commercially available pseudomonas, 3 parts of streptomyces microflavus, 8 parts of brevibacillus laterosporus, 13 parts of bacillus mucilaginosus, 2 parts of phosphate-solubilizing potassium-solubilizing bacteria, 6 parts of bacillus subtilis, 8 parts of candida tropicalis, 9 parts of bacillus licheniformis, 7 parts of bacillus megaterium, 9 parts of cellulose streptomyces and 2 parts of rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and then mixing the composite bacteria with sucrose or fructo-oligosaccharide according to a weight ratio of 1:5 to obtain a composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and the crushed corncobs are mixed according to the weight ratio of 1:25, the humidity is adjusted to 55%, and the corncobs are fermented and stored for 20 days at the temperature of 45 ℃ to obtain a corncob yeast storage compounding agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) are mixed with the crushed corn straws according to the proportion of 1:25, the humidity is adjusted to 60%, and the corn straw fermented material mixed agent is obtained after fermentation storage for 25 days at the temperature of 65 ℃;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing water at 75 ℃, and naturally cooling to obtain a sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Comparative example 1
The difference from the example 3 is only that the corn cob fermentation storage compound agent, the corn stalk decomposition agent and the sweet potato powder compound agent are respectively replaced by the corn cob fermentation storage compound agent, the corn stalk decomposition agent and the sweet potato powder compound agent which are equally divided into unprocessed corn cobs, corn stalks and sweet potato powder.
Comparative example 2
The only difference from example 3 is that no corncob ferment storage compound was added.
Comparative example 3
The only difference from example 3 is that no corn stover decomposing agent was added.
Comparative example 4
The only difference from example 3 is that no sweet potato flour combination was added.
Comparative example 5
The difference from example 3 is only that sucrose or fructo-oligosaccharide is not added when the complex microbial inoculum is prepared, but is added in the process of preparing the fertilizer.
And (3) testing: and (3) applying 100g of fertilizers in examples 1-8 and comparative examples 1-5 respectively to each group, sowing 10 cucumber seeds in each pot, planting and reserving 3 cucumber seeds after seedling emergence, wherein the illumination time intensities are the same, sowing is carried out in No. 6 and No. 19 in 2019, harvesting is carried out in No. 7 and No. 23 in 2019, and the plant height, the dry weight, the average weight of each cucumber and the average number of each cucumber are measured, and the cucumber variety is the No. four cucumber in Jinchun.
In addition, a control group and a blank group are arranged, wherein the control group adopts a common fertilizer on the market, namely a special fertilizer for Fujian cucumber, and the blank group does not apply the fertilizer.
TABLE 1 cucumber growth Using the fertilizers prepared in examples 1-8
TABLE 2 cucumber growth using the fertilizers prepared in comparative examples 1-5 and the control and blank groups
Compared with the blank group and the control group, the biofertilizers prepared by the invention can obviously promote the growth of cucumber seedlings and greatly improve the yield of cucumbers through the examples 1-8, which shows that the biofertilizers can effectively improve the soil fertility and is beneficial to the growth of cucumbers.
Compared with the comparative example 1, the embodiment shows that the corncobs, the corn straws and the sweet potato powder are pretreated and then used for preparing the biological fertilizer, the using effect is better, and the corncobs, the corn straws and the sweet potato powder can be more fully fermented and decomposed by treating with the composite microbial inoculum and the amylase, so that the nutrients more favorable for the cucumber seedling to absorb are obtained.
Compared with the comparative examples 2-4, the application effect is the best only when the corncob ferment storage compound agent, the corn stalk decomposition agent and the sweet potato powder compound agent are added at the same time, which shows that the components in the fertilizer have synergistic effect and can greatly improve the quality of cucumbers.
Compared with the comparative example 5, the effect of adding the sucrose or the fructo-oligosaccharide during the preparation of the composite microbial inoculum is better, probably because the sucrose or the fructo-oligosaccharide provides a carbon source for the life activity of microorganisms, the strain is protected; if the compound microbial inoculum is not added with sucrose or fructo-oligosaccharide, the competition effect of the prepared compound microbial inoculum among strains in the storage process is more obvious, and each strain can generate adverse effect, thereby reducing the use effect of the fertilizer.
Moreover, cucumbers planted by the fertilizers prepared in the embodiments 1 to 8 have uniform shape and color, and fine and smooth mouthfeel, and are crisp and sweet.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A special biofertilizer for cucumber seedlings is characterized in that: the compound is prepared from the following raw materials in parts by weight: 4-12 parts of attapulgite, 4-15 parts of rapeseed cakes, 4-23 parts of a corn cob fermentation storage compound agent, 2-26 parts of a corn straw decomposition agent, 3-19 parts of a sweet potato flour compound agent and 1-5 parts of a compound microbial inoculum.
2. The biofertilizer special for cucumber seedlings as claimed in claim 1, characterized in that: the corn cob fermentation storage compound agent is obtained by mixing the compound microbial inoculum and crushed corn cobs according to the weight ratio of 1:25, adjusting the humidity to 50-70%, and fermenting and storing for 15-20 days at the temperature of 35-45 ℃.
3. The biofertilizer special for cucumber seedlings as claimed in claim 1, characterized in that: the corn straw decomposing inoculant is obtained by mixing the composite inoculant and crushed corn straws in a weight ratio of 1:25, adjusting the humidity to 50-70%, and fermenting at the temperature of 55-65 ℃ for 25-30 days.
4. The biofertilizer special for cucumber seedlings as claimed in claim 1, characterized in that: the sweet potato powder compound agent is prepared by mixing medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the material-water ratio is 4: 1, uniformly mixing the mixture with water at the temperature of 60-75 ℃, and naturally cooling the mixture to obtain the product.
5. The biofertilizer special for cucumber seedlings as claimed in claim 1, characterized in that: the composite microbial inoculum comprises the following raw materials in parts by weight: 3-7 parts of pseudomonas, 2-7 parts of streptomyces microflavus, 3-11 parts of brevibacillus laterosporus, 3-13 parts of bacillus mucilaginosus, 1-7 parts of phosphate-solubilizing potassium-solubilizing bacteria, 2-15 parts of bacillus subtilis, 1-8 parts of candida tropicalis, 3-9 parts of bacillus licheniformis, 2-9 parts of bacillus megaterium, 1-9 parts of cellulose streptomyces and 2-8 parts of rhodopseudomonas palustris; the strain also comprises sucrose or fructo-oligosaccharide, and the weight ratio of the sucrose or fructo-oligosaccharide to the mixed strain is 5: 1.
6. A method for preparing a biofertilizer for cucumber seedlings according to any one of claims 1 to 5, characterized in that: the method comprises the following steps:
1) preparation of composite microbial inoculum
Taking commercially available pseudomonas, streptomyces microflavus, brevibacillus laterosporus, bacillus mucilaginosus, phosphorus and potassium solubilizing bacteria, bacillus subtilis, candida tropicalis, bacillus licheniformis, bacillus megaterium, streptomyces cellulosae and rhodopseudomonas palustris, uniformly mixing to obtain composite bacteria, and then mixing the composite bacteria with sucrose or fructo-oligosaccharide according to the weight part ratio of 1:5 to obtain the composite microbial agent;
2) preparation of corncob ferment storing compound agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and crushed corncobs are mixed according to the weight ratio of 1:25, the humidity is adjusted to 50-70%, and the corncobs are fermented and stored for 15-20 days at the temperature of 35-45 ℃ to obtain a corncob fermented and stored compound agent;
3) preparation of corn stalk decomposition agent
1/4 parts by weight of the composite microbial inoculum prepared in the step 1) and crushed corn straws are mixed according to the weight ratio of 1:25, the humidity is adjusted to 50-70%, and the corn straws are fermented and stored for 25-30 days at the temperature of 55-65 ℃ to obtain a corn straw fermented and stored compound agent;
4) preparation of sweet potato powder compound agent
Mixing the medium-temperature alpha-amylase and sweet potato powder according to the weight ratio of 1:1500, wherein the ratio of material to water is 4: 1, uniformly mixing water at the temperature of 60-75 ℃, and naturally cooling to obtain a sweet potato powder compound agent;
5) uniformly mixing the attapulgite, the rapeseed cake, the corncob fermentation storage compound agent, the corn straw decomposition agent, the sweet potato powder compound agent and the residual compound microbial inoculum according to the formula ratio to obtain the fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110449327.7A CN113072408B (en) | 2021-04-25 | 2021-04-25 | Special biofertilizer for cucumber seedlings and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110449327.7A CN113072408B (en) | 2021-04-25 | 2021-04-25 | Special biofertilizer for cucumber seedlings and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113072408A true CN113072408A (en) | 2021-07-06 |
| CN113072408B CN113072408B (en) | 2022-07-01 |
Family
ID=76618815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110449327.7A Active CN113072408B (en) | 2021-04-25 | 2021-04-25 | Special biofertilizer for cucumber seedlings and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113072408B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104529603A (en) * | 2014-10-14 | 2015-04-22 | 马鞍山科邦生态肥有限公司 | Cucumber coated slow release fertilizer and preparation method thereof |
| CN104710222A (en) * | 2013-12-15 | 2015-06-17 | 张元宝 | Biological fertilizer for crops |
| CN105315057A (en) * | 2014-07-30 | 2016-02-10 | 重庆市萍萌农业开发有限公司 | Multielement compounded biofertilizer and preparation method therefor |
| CN106116889A (en) * | 2016-06-29 | 2016-11-16 | 山东省农业科学院蔬菜花卉研究所 | A kind of green cucumber special fertilizer |
| CN106554235A (en) * | 2016-12-05 | 2017-04-05 | 安徽爱能洁生物科技有限公司 | A kind of nutrition grape special fertilizer in a balanced way |
| CN106986705A (en) * | 2017-05-06 | 2017-07-28 | 安徽帝都农业生态园有限公司 | A kind of composite fertilizer and preparation method thereof |
| CN107056532A (en) * | 2017-03-13 | 2017-08-18 | 安徽省卫国农业科技有限公司 | A kind of organic fertilizer of promotion cucumber growth |
| CN108314545A (en) * | 2018-04-19 | 2018-07-24 | 合肥市风达农业有限责任公司 | A kind of green cucumber dedicated fertilizer and preparation method thereof |
-
2021
- 2021-04-25 CN CN202110449327.7A patent/CN113072408B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104710222A (en) * | 2013-12-15 | 2015-06-17 | 张元宝 | Biological fertilizer for crops |
| CN105315057A (en) * | 2014-07-30 | 2016-02-10 | 重庆市萍萌农业开发有限公司 | Multielement compounded biofertilizer and preparation method therefor |
| CN104529603A (en) * | 2014-10-14 | 2015-04-22 | 马鞍山科邦生态肥有限公司 | Cucumber coated slow release fertilizer and preparation method thereof |
| CN106116889A (en) * | 2016-06-29 | 2016-11-16 | 山东省农业科学院蔬菜花卉研究所 | A kind of green cucumber special fertilizer |
| CN106554235A (en) * | 2016-12-05 | 2017-04-05 | 安徽爱能洁生物科技有限公司 | A kind of nutrition grape special fertilizer in a balanced way |
| CN107056532A (en) * | 2017-03-13 | 2017-08-18 | 安徽省卫国农业科技有限公司 | A kind of organic fertilizer of promotion cucumber growth |
| CN106986705A (en) * | 2017-05-06 | 2017-07-28 | 安徽帝都农业生态园有限公司 | A kind of composite fertilizer and preparation method thereof |
| CN108314545A (en) * | 2018-04-19 | 2018-07-24 | 合肥市风达农业有限责任公司 | A kind of green cucumber dedicated fertilizer and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113072408B (en) | 2022-07-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101880197B (en) | Microbial compound fertilizer and application thereof | |
| CN103848698B (en) | A kind of biological organic fertilizer utilizing natural pond slag aerobic fermentation to prepare and preparation method thereof | |
| US8530220B2 (en) | Microorganisms, microbial phosphate fertilizers and methods for preparing such microbial phosphate fertilizers | |
| CN103031254B (en) | Efficient straw-decomposing inoculant and preparation method thereof | |
| CN105036921A (en) | Plant nutrient solution and preparing method thereof | |
| CN109402012A (en) | It is a kind of to prevent and treat the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease, biological organic fertilizer and preparation method thereof | |
| CN110590457A (en) | Biological organic bacterial fertilizer for crops and preparation method thereof | |
| CN108276231A (en) | A kind of Soil organic phosphorus fractions modifying agent, preparation method and applications | |
| CN104030794A (en) | Preparation method of wuta-tsai dedicated organic matrix | |
| CN104692961A (en) | Microorganism organic material decomposition agent as well as preparation method and applications thereof | |
| CN112624866A (en) | Organic slow release fertilizer using livestock and poultry manure and straw as raw materials | |
| CN113135787A (en) | Rice straw decomposition agent and preparation method thereof | |
| CN114230385A (en) | Special microbial fertilizer for sweet sorghum in saline-alkali soil and preparation method thereof | |
| CN103351188B (en) | Preparation method of agricultural organic fertilizer by treating pure straws as raw material | |
| CN110959501A (en) | Rice seedling raising substrate | |
| CN109627099A (en) | A kind of spherical particle alkaline conditioner and its preparation | |
| CN101928161A (en) | Method for preparing direct seeding compression nutrition pot raw material | |
| US20240002308A1 (en) | Soil-structure improving bio-organic fertilizer and preparation method thereof | |
| CN111876342A (en) | Low-temperature straw decomposing microbial inoculum and preparation method and application thereof | |
| CN111607404A (en) | Preparation method of probiotic soil conditioner and product thereof | |
| CN109197429B (en) | Method for cultivating Shanghai green by utilizing wormcast produced by sugar refinery waste materials | |
| CN110903128A (en) | Rice straw organic fertilizer and preparation method thereof | |
| CN111099937A (en) | Method for preparing organic fertilizer by using EM (effective microorganisms) stock solution | |
| CN111034407A (en) | Microbial utilization technology for returning rice straws to field in northeast region | |
| CN113072408B (en) | Special biofertilizer for cucumber seedlings and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |