CN113026114B - 缓冲液及其应用 - Google Patents
缓冲液及其应用 Download PDFInfo
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- CN113026114B CN113026114B CN201911353781.1A CN201911353781A CN113026114B CN 113026114 B CN113026114 B CN 113026114B CN 201911353781 A CN201911353781 A CN 201911353781A CN 113026114 B CN113026114 B CN 113026114B
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- 239000007853 buffer solution Substances 0.000 title claims abstract description 41
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- 238000010276 construction Methods 0.000 claims abstract description 20
- 239000007983 Tris buffer Substances 0.000 claims abstract description 8
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims abstract description 8
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000004094 surface-active agent Substances 0.000 claims abstract description 7
- 239000000872 buffer Substances 0.000 claims description 37
- 230000008439 repair process Effects 0.000 claims description 20
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 12
- 102000039446 nucleic acids Human genes 0.000 claims description 12
- 150000007523 nucleic acids Chemical class 0.000 claims description 12
- 238000013467 fragmentation Methods 0.000 claims description 10
- 238000006062 fragmentation reaction Methods 0.000 claims description 10
- 230000006154 adenylylation Effects 0.000 claims description 7
- 239000002202 Polyethylene glycol Substances 0.000 claims description 6
- 229920001223 polyethylene glycol Polymers 0.000 claims description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 5
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 3
- -1 ethyl phenyl Chemical group 0.000 claims description 3
- ZPIRTVJRHUMMOI-UHFFFAOYSA-N octoxybenzene Chemical compound CCCCCCCCOC1=CC=CC=C1 ZPIRTVJRHUMMOI-UHFFFAOYSA-N 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 22
- 238000003541 multi-stage reaction Methods 0.000 abstract description 14
- 102000012410 DNA Ligases Human genes 0.000 abstract description 9
- 108010061982 DNA Ligases Proteins 0.000 abstract description 9
- 238000000746 purification Methods 0.000 abstract description 9
- 230000035484 reaction time Effects 0.000 abstract description 7
- 230000002401 inhibitory effect Effects 0.000 abstract description 5
- 230000002349 favourable effect Effects 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 33
- 238000006243 chemical reaction Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 239000012634 fragment Substances 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 5
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 5
- 238000007259 addition reaction Methods 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 5
- 229910001425 magnesium ion Inorganic materials 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 3
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910001424 calcium ion Inorganic materials 0.000 description 3
- 239000011777 magnesium Substances 0.000 description 3
- 230000001603 reducing effect Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- LBCZOTMMGHGTPH-UHFFFAOYSA-N 2-[2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCO)C=C1 LBCZOTMMGHGTPH-UHFFFAOYSA-N 0.000 description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000007481 next generation sequencing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 229950006790 adenosine phosphate Drugs 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000003766 bioinformatics method Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910001437 manganese ion Inorganic materials 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000011191 terminal modification Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201911353781.1A CN113026114B (zh) | 2019-12-25 | 2019-12-25 | 缓冲液及其应用 |
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CN201911353781.1A CN113026114B (zh) | 2019-12-25 | 2019-12-25 | 缓冲液及其应用 |
Publications (2)
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CN113026114A CN113026114A (zh) | 2021-06-25 |
CN113026114B true CN113026114B (zh) | 2022-10-28 |
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CN201911353781.1A Active CN113026114B (zh) | 2019-12-25 | 2019-12-25 | 缓冲液及其应用 |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6060249A (en) * | 1997-02-27 | 2000-05-09 | Genentech, Inc. | Method of selection for genes encoding secreted and transmembrane proteins |
CN1358232A (zh) * | 1999-06-24 | 2002-07-10 | 卡维迪技术有限公司 | 反转录酶分析试剂盒及其使用以及用于在生物样品中进行rt活性分析的方法 |
CN103476428A (zh) * | 2010-09-09 | 2013-12-25 | 北京同为时代生物技术有限公司 | 用于诊断上皮源性癌症的血液标志物及单克隆抗体 |
CN105603535A (zh) * | 2016-01-27 | 2016-05-25 | 北京诺禾致源生物信息科技有限公司 | 构建dna文库的试剂盒和方法 |
CN110468123A (zh) * | 2018-05-11 | 2019-11-19 | 南京理工大学 | 莱茵衣藻核基因组pcr体系 |
-
2019
- 2019-12-25 CN CN201911353781.1A patent/CN113026114B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6060249A (en) * | 1997-02-27 | 2000-05-09 | Genentech, Inc. | Method of selection for genes encoding secreted and transmembrane proteins |
CN1358232A (zh) * | 1999-06-24 | 2002-07-10 | 卡维迪技术有限公司 | 反转录酶分析试剂盒及其使用以及用于在生物样品中进行rt活性分析的方法 |
CN103476428A (zh) * | 2010-09-09 | 2013-12-25 | 北京同为时代生物技术有限公司 | 用于诊断上皮源性癌症的血液标志物及单克隆抗体 |
CN105603535A (zh) * | 2016-01-27 | 2016-05-25 | 北京诺禾致源生物信息科技有限公司 | 构建dna文库的试剂盒和方法 |
CN110468123A (zh) * | 2018-05-11 | 2019-11-19 | 南京理工大学 | 莱茵衣藻核基因组pcr体系 |
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CN113026114A (zh) | 2021-06-25 |
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Effective date of registration: 20220922 Address after: 322000 1st floor, building 9, standard workshop, No.10 Gaoxin Road, Houjiang street, Yiwu City, Jinhua City, Zhejiang Province Applicant after: ZHEJIANG ANNOROAD BIO-TECHNOLOGY Co.,Ltd. Applicant after: ANNOROAD GENE TECHNOLOGY (BEIJING) Co.,Ltd. Address before: Room 701, unit 2, building 8, yard 88, Kechuang 6th Street, Daxing District, Beijing 100176 Applicant before: ANNOROAD GENE TECHNOLOGY (BEIJING) Co.,Ltd. |
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Effective date of registration: 20240626 Address after: Room 101 and 201, Unit 2, Building 8, No. 88 Kechuang 6th Street, Beijing Economic and Technological Development Zone, Daxing District, Beijing, 100176 Patentee after: BEIJING ANNOROAD MEDICAL LABORATORY Co.,Ltd. Country or region after: China Patentee after: ANNOROAD GENE TECHNOLOGY (BEIJING) Co.,Ltd. Address before: 322000 1st floor, building 9, standard workshop, No.10 Gaoxin Road, Houjiang street, Yiwu City, Jinhua City, Zhejiang Province Patentee before: ZHEJIANG ANNOROAD BIO-TECHNOLOGY Co.,Ltd. Country or region before: China Patentee before: ANNOROAD GENE TECHNOLOGY (BEIJING) Co.,Ltd. |