CN113025479A - Kit for detecting cell activation antigen - Google Patents

Kit for detecting cell activation antigen Download PDF

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Publication number
CN113025479A
CN113025479A CN202110222227.0A CN202110222227A CN113025479A CN 113025479 A CN113025479 A CN 113025479A CN 202110222227 A CN202110222227 A CN 202110222227A CN 113025479 A CN113025479 A CN 113025479A
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China
Prior art keywords
storage box
kit
fixedly connected
cell activation
reagent tube
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Pending
Application number
CN202110222227.0A
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Chinese (zh)
Inventor
许恩顺
梁宁孝
王海珍
何奕辉
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Nanjing Diji Biotechnology Co ltd
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Nanjing Diji Biotechnology Co ltd
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Priority to CN202110222227.0A priority Critical patent/CN113025479A/en
Publication of CN113025479A publication Critical patent/CN113025479A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827Total protein determination, e.g. albumin in urine
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • B01L3/50825Closing or opening means, corks, bungs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kit for detecting cell activation antigen, wherein a box cover is hinged with a storage box, and is positioned above the storage box, each reagent tube is detachably connected with the storage box, and are respectively positioned in the corresponding storage grooves, two groups of fixing components are arranged on each reagent tube, each group of fixing components respectively comprises a movable plate, a movable spring and a clamping block, one end of the movable plate is fixedly connected with the reagent tube, and is positioned on the outer side wall of the reagent tube, one end of the movable spring is fixedly connected with the reagent tube, and is positioned on the outer side wall of the reagent tube, the other end of the movable spring is fixedly connected with the movable plate, the clamping block is fixedly connected with the movable plate, and is located the lateral wall of fly leaf, just the fixture block with draw-in groove looks adaptation realizes improving medical personnel's use through the setting of above-mentioned structure and feels.

Description

Kit for detecting cell activation antigen
Technical Field
The invention relates to the technical field of medical detection equipment, in particular to a kit for detecting cell activation antigen.
Background
Cellular immunity refers broadly to an immune response enhanced by specific cellular and non-specific cellular activities, and refers narrowly to T cell-mediated immunity. The cellular immunity refers to the direct killing effect of the sensitized T cells on the antigen and the synergistic killing effect of cytokines released by the sensitized T cells when the same antigen re-enters the cells of the body, which are collectively called cellular immunity. Activation of immune cells is a marker for the development of an immune response. After the resting cells are activated by antigen stimulation, a series of characteristics are shown, such as expression of cell membrane surface activating molecules and the like.
Medical personnel add different reagents in the reagent pipe and detect the nucleic acid or the protein amount of every different test body to put into the reagent box, when medical personnel used kit and reagent pipe at present, the use was felt not good.
Disclosure of Invention
The invention aims to provide a kit for detecting a cell activation antigen, and aims to solve the technical problem that medical staff in the prior art are poor in use feeling.
In order to achieve the purpose, the kit for detecting cell activation antigen adopted by the invention comprises a storage box, a box cover, reagent tubes and fixing components, wherein the box cover is hinged with the storage box and is positioned above the storage box, a plurality of storage grooves and a plurality of clamping grooves are formed in the storage box, each clamping groove is respectively communicated with the corresponding storage groove, a plurality of reagent tubes are detachably connected with the storage box and are respectively positioned in the corresponding storage grooves, and two groups of fixing components are arranged on each reagent tube;
each group of fixing components respectively comprises a movable plate, a movable spring and a clamping block, one end of the movable plate is fixedly connected with the reagent tube and is positioned on the outer side wall of the reagent tube, one end of the movable spring is fixedly connected with the reagent tube and is positioned on the outer side wall of the reagent tube, the other end of the movable spring is fixedly connected with the movable plate, the clamping block is fixedly connected with the movable plate and is positioned on the outer side wall of the movable plate, and the clamping block is matched with the clamping groove.
Each reagent tube comprises an outer tube, an inner tube and a plug block, the outer tube is detachably connected with the storage box and is positioned in the storage groove, the inner tube is fixedly connected with the outer tube and is embedded in the inner side wall of the outer tube, and the plug blocks are detachably connected with the inner tube.
The reagent tube further comprises a sealing ring, and the sealing ring is fixedly connected with the plugging block and is positioned below the plugging block.
The kit for detecting the cell activation antigen further comprises anti-skid blocks, anti-skid lines are arranged on the anti-skid blocks, and each movable plate is provided with the anti-skid block.
The kit for detecting the cell activation antigen further comprises a sliding plate and an observation plate, wherein the storage box is provided with a sliding groove and a placing groove, the sliding groove is communicated with the placing groove, the observation plate is fixedly connected with the storage box and is positioned in the placing groove, and the sliding plate is slidably connected with the storage box and is positioned in the sliding groove.
The kit for detecting the cell activation antigen further comprises a friction pad, wherein the friction pad is fixedly connected with the storage box and is positioned below the storage box.
The invention has the beneficial effects that: when medical personnel use the kit for detecting cell activation antigen to detect nucleic acid or protein amount of different test bodies, the box cover and the reagent tubes are opened, the test bodies are respectively placed into the different reagent tubes fixed in the storage tank, different reagents are added into the reagent tubes, the reagent tubes and the box cover are covered, the reagents and the test bodies react automatically, after the reaction is carried out for a proper time, the medical personnel open the box cover, open one of the reagent tubes, take out some test bodies by using a cotton swab or a dropper, detect the nucleic acid or protein amount of the test bodies by using a detection instrument, and clamp the clamp block in the clamp groove in the process of opening the reagent tubes, so that the reagent tubes and the storage tank are relatively fixed, and the reagent tubes are prevented from being taken out of the storage tank when the reagent tubes are opened, the medical staff detects the test body in each reagent tube in turn; after the detection is finished, the movable plate on one of the reagent tubes is pinched by hand, force is applied to enable the movable plate to be attached to the reagent tube, the clamping block is separated from the clamping groove, medical workers can take out the reagent tubes, clean and sterilize the reagent tubes, sequentially take out the used reagent tubes, and perform cleaning and sterilizing treatment, so that preparation is made for next detection, and the use feeling of the medical workers is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a partial structural view of the kit for detecting a cell activation antigen according to the present invention.
FIG. 2 is a front view of the kit for detecting cell activation antigen of the present invention.
Fig. 3 is an enlarged view of a portion of the structure of fig. 1 according to the present invention.
FIG. 4 is another schematic structural view of a portion of the kit for detecting a cell activation antigen according to the present invention.
100-a kit for detecting cell activation antigen, 1-a storage box, 2-a box cover, 3-a reagent tube, 4-a fixed component, 5-an antiskid block, 6-a sliding plate, 7-an observation plate, 8-a spring component, 9-a buffer strip, 10-a storage groove, 11-a clamping groove, 12-a sliding groove, 13-a placing groove, 14-a limiting groove, 15-an antiskid line, 16-a friction pad, 31-an outer cylinder, 32-an inner tube, 33-a plug block, 34-a sealing ring, 41-a movable plate, 42-a movable spring, 43-a clamping block, 81-a return spring and 82-a propping cylinder.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 4, the present invention provides a kit 100 for detecting a cell activation antigen, including a storage box 1, a box cover 2, a reagent tube 3 and a fixing component 4, wherein the box cover 2 is hinged to the storage box 1 and is located above the storage box 1, the storage box 1 has a plurality of storage slots 10 and a plurality of clamping slots 11 therein, each clamping slot 11 is respectively communicated with the corresponding storage slot 10, the number of the reagent tubes 3 is multiple, each reagent tube 3 is detachably connected to the storage box 1 and is respectively located in the corresponding storage slot 10, and each reagent tube 3 is provided with two sets of the fixing components 4;
each group of the fixing components 4 respectively includes a movable plate 41, a movable spring 42 and a clamping block 43, one end of the movable plate 41 is fixedly connected with the reagent tube 3 and is located on the outer side wall of the reagent tube 3, one end of the movable spring 42 is fixedly connected with the reagent tube 3 and is located on the outer side wall of the reagent tube 3, the other end of the movable spring 42 is fixedly connected with the movable plate 41, the clamping block 43 is fixedly connected with the movable plate 41 and is located on the outer side wall of the movable plate 41, and the clamping block 43 is matched with the clamping groove 11.
In this embodiment, when a medical staff uses the kit 100 for detecting cell activation antigen to detect the amount of nucleic acid or protein in different test bodies, the box cover 2 and the reagent tube 3 are opened, the test bodies are respectively put into the different reagent tubes 3 fixed in the storage tank 10, different reagents are added into the reagent tubes 3, the reagent tubes 3 and the box cover 2 are covered to allow the reagents and the test bodies to react with each other, after a proper time of reaction, the medical staff opens the box cover 2, opens one of the reagent tubes 3, takes out some test bodies with a cotton swab or a dropper, detects the amount of nucleic acid or protein in the test bodies with a detection instrument, and in the process of opening the reagent tubes 3, the block 43 is clamped in the clamping groove 11, so that the reagent tubes 3 and the storage box 1 are relatively fixed, and when the reagent tubes 3 are opened, taking the reagent tubes 3 out of the storage groove 10, and sequentially detecting the test body in each reagent tube 3 by medical staff; after the detection is finished, the movable plate 41 on one of the reagent tubes 3 is pinched by hand, and a force is applied to make the movable plate 41 fit to the reagent tube 3, at this time, the clamping block 43 is separated from the clamping groove 11, the medical staff can take out the reagent tube 3, perform cleaning and sterilizing treatment on the reagent tube 3, sequentially take out each used reagent tube 3, perform cleaning and sterilizing treatment, so as to prepare for the next detection, and improve the use feeling of the medical staff.
Further, each reagent tube 3 comprises an outer tube 31, an inner tube 32 and a plug 33, the outer tube 31 is detachably connected with the storage box 1 and is located in the storage tank 10, the inner tube 32 is fixedly connected with the outer tube 31 and is embedded in the inner side wall of the outer tube 31, and the plug 33 is detachably connected with the inner tube 32.
Further, the reagent tube 3 further comprises a sealing ring 34, and the sealing ring 34 is fixedly connected with the plug block 33 and is located below the plug block 33.
In this embodiment, when a medical staff uses the kit 100 for detecting cell activation antigen to detect nucleic acid or protein amounts of different test bodies, the box cover 2 and the plug 33 are opened, the test bodies are respectively put into the different inner tubes 32 fixed in the storage tank 10, different reagents are added to the inner tubes 32, the plug 33 and the box cover 2 are closed, the reagents and the test bodies are allowed to react with each other, and the sealing ring 34 increases the sealing performance of the plug 33 and the inner tubes 32.
Further, the kit 100 for detecting cell activation antigen further comprises an anti-skid block 5, the anti-skid block 5 is provided with anti-skid lines 15, and each movable plate 41 is provided with the anti-skid block 5.
In this embodiment, after the detection is completed, the anti-slip block 5 of the movable plate 41 on one of the outer cylinders 31 is pinched by hand, and a force is applied to attach the movable plate 41 to the outer cylinder 31, at this time, the fixture block 43 is separated from the clamping groove 11, so that the medical staff can take out the outer cylinder 31, perform cleaning and sterilizing treatment on the inner tubes 32, sequentially take out each used inner tube 32, perform cleaning and sterilizing treatment to prepare for the next detection, and the anti-slip block 5 is provided with the anti-slip lines 15 to prevent the medical staff from slipping hands when taking out the outer cylinder 31 and breaking the outer cylinder 31.
Further, the kit 100 for detecting cell activation antigen further comprises a sliding plate 6 and an observation plate 7, the storage box 1 is provided with a sliding groove 12 and a placing groove 13, the sliding groove 12 is communicated with the placing groove 13, the observation plate 7 is fixedly connected with the storage box 1 and is positioned in the placing groove 13, and the sliding plate 6 is slidably connected with the storage box 1 and is positioned in the sliding groove 12.
In this embodiment, observation board 7 is transparent material, and medical personnel puts the test body into respectively and fixes differently in the inner tube 32, and toward add different reagents in the inner tube 32, cover again chock 33 with lid 2 lets reagent and test body react by oneself, and in the reaction process, medical personnel slidable sliding plate 6 is to exposing observation board 7, through observation board 7 observes the reaction effect.
Further, the kit 100 for detecting cell activation antigen further comprises a friction pad 16, wherein the friction pad 16 is fixedly connected with the storage box 1 and is positioned below the storage box 1.
In this embodiment, the friction pad 16 is located below the storage box 1, so as to increase the friction between the storage box 1 and the tabletop or the ground, and prevent medical personnel from accidentally touching the storage box 1, which may cause the storage box 1 to shake due to position deviation, thereby affecting the reaction result.
Further, the kit 100 for detecting cell activation antigen further includes an elastic component 8, the elastic component 8 is disposed in each storage groove 10, the elastic component 8 includes a return spring 81 and a supporting cylinder 82, the outer cylinder 31 has a limiting groove 14, one end of the return spring 81 is fixedly connected to the storage box 1 and is located in the storage groove 10, the other end of the return spring 81 is fixedly connected to the supporting cylinder 82 and is located on the inner side wall of the supporting cylinder 82, and the supporting cylinder 82 is adapted to the limiting groove 14.
Further, the kit 100 for detecting cell activation antigen further comprises a buffer strip 9, and the buffer strip 9 is fixedly connected with the outer cylinder 31 and is located below the outer cylinder 31.
In this embodiment, when the outer cylinder 31 is placed in the storage tank 10, the outer cylinder 31 presses the abutting cylinder 82, so that the return spring 81 has a resilient force, when a medical worker prepares to take out the outer cylinder 31, the medical worker pinches the anti-slip block 5 on the movable plate 41 with a hand and applies a force to make the movable plate 41 fit to the outer cylinder 31, at this time, the latch 43 is disengaged from the slot 11, the abutting cylinder 82 pushes the outer cylinder 31 under the resilient force of the return spring 81, so that the medical worker can easily take out the outer cylinder 31 and perform cleaning and sterilization treatment on the inner tube 32; when the medical staff prepares to put back the outer barrel 31, the outer barrel 31 is aligned to the storage groove 10, the outer barrel 31 is pushed downwards until the abutting barrel 82 is embedded in the limiting groove 14, the outer barrel 31 extrudes the abutting barrel 82, when the clamping block 43 is clamped in the clamping groove 11, the outer barrel 31 and the storage box 1 are relatively fixed, the buffer strip 9 buffers the extrusion of the outer barrel 31 on the storage box 1, and the damage of the outer barrel 31 caused by overlarge force of the medical staff for extruding the outer barrel 31 is avoided.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (6)

1. A kit for detecting a cell activation antigen, characterized in that,
the reagent tube fixing device comprises a storage box, a box cover, reagent tubes and fixing components, wherein the box cover is hinged with the storage box and is positioned above the storage box, a plurality of storage grooves and a plurality of clamping grooves are formed in the storage box, each clamping groove is respectively communicated with the corresponding storage groove, the number of the reagent tubes is multiple, each reagent tube is detachably connected with the storage box and is respectively positioned in the corresponding storage groove, and two groups of the fixing components are arranged on each reagent tube;
each group of fixing components respectively comprises a movable plate, a movable spring and a clamping block, one end of the movable plate is fixedly connected with the reagent tube and is positioned on the outer side wall of the reagent tube, one end of the movable spring is fixedly connected with the reagent tube and is positioned on the outer side wall of the reagent tube, the other end of the movable spring is fixedly connected with the movable plate, the clamping block is fixedly connected with the movable plate and is positioned on the outer side wall of the movable plate, and the clamping block is matched with the clamping groove.
2. The kit for detecting a cell activation antigen according to claim 1,
each reagent pipe comprises an outer barrel, an inner pipe and a plug block, the outer barrel is detachably connected with the storage box and is located in the storage groove, the inner pipe is fixedly connected with the outer barrel and is embedded in the inner side wall of the outer barrel, and the plug block is detachably connected with the inner pipe.
3. The kit for detecting a cell activation antigen according to claim 2,
the reagent tube further comprises a sealing ring, and the sealing ring is fixedly connected with the plugging block and is positioned below the plugging block.
4. The kit for detecting a cell activation antigen according to claim 1,
the kit for detecting the cell activation antigen further comprises anti-skid blocks, anti-skid lines are arranged on the anti-skid blocks, and each movable plate is provided with the anti-skid block.
5. The kit for detecting a cell activation antigen according to claim 3,
the kit for detecting the cell activation antigen further comprises a sliding plate and an observation plate, wherein the storage box is provided with a sliding groove and a placing groove, the sliding groove is communicated with the placing groove, the observation plate is fixedly connected with the storage box and is positioned in the placing groove, and the sliding plate is slidably connected with the storage box and is positioned in the sliding groove.
6. The kit for detecting a cell activation antigen according to claim 1,
the kit for detecting the cell activation antigen further comprises a friction pad, wherein the friction pad is fixedly connected with the storage box and is positioned below the storage box.
CN202110222227.0A 2021-02-28 2021-02-28 Kit for detecting cell activation antigen Pending CN113025479A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110222227.0A CN113025479A (en) 2021-02-28 2021-02-28 Kit for detecting cell activation antigen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110222227.0A CN113025479A (en) 2021-02-28 2021-02-28 Kit for detecting cell activation antigen

Publications (1)

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CN113025479A true CN113025479A (en) 2021-06-25

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CN202110222227.0A Pending CN113025479A (en) 2021-02-28 2021-02-28 Kit for detecting cell activation antigen

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113716199A (en) * 2021-09-14 2021-11-30 南京优而生物科技发展有限公司 Safety detection method of liquid reagent and transfer device thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN205528791U (en) * 2016-04-29 2016-08-31 宁波市瑞德曼医疗投资管理有限公司 Kit for detecting cell activation antigen
CN210364922U (en) * 2019-06-18 2020-04-21 华夏源(上海)细胞基因工程股份有限公司 CIK cell activation kit
CN211002443U (en) * 2019-11-19 2020-07-14 南京菲尔特生物技术有限公司 Animal and plant genome DNA small-amount kit

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN205528791U (en) * 2016-04-29 2016-08-31 宁波市瑞德曼医疗投资管理有限公司 Kit for detecting cell activation antigen
CN210364922U (en) * 2019-06-18 2020-04-21 华夏源(上海)细胞基因工程股份有限公司 CIK cell activation kit
CN211002443U (en) * 2019-11-19 2020-07-14 南京菲尔特生物技术有限公司 Animal and plant genome DNA small-amount kit

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113716199A (en) * 2021-09-14 2021-11-30 南京优而生物科技发展有限公司 Safety detection method of liquid reagent and transfer device thereof

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